Human CD14 Quantikine ELISA Kit



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Reactivity HuSpecies Glossary
Applications ELISA

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Human CD14 Quantikine ELISA Kit Summary

The Quantikine Human soluble CD14 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human soluble CD14 in cell culture supernates, serum, and plasma. It contains recombinant human CD14 expressed from CHO cells and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained measuring natural ...human sCD14 showed dose-response curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human sCD14.
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Natural and recombinant human sCD14
Assay Type
Solid Phase Sandwich ELISA
See PDF Datasheet for details
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details


Application Notes
No significant interference observed with available related molecules.
Read Publications using DC140.

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CD14 Quantikine ELISA Kit

  • CD14 antigen
  • CD14 molecule
  • CD14
  • monocyte differentiation antigen CD14
  • Myeloid cell-specific leucine-rich glycoprotein


CD14 is an acute phase glycoprotein that binds lipopolysaccharide endotoxins (LPS) from Gram-negative bacteria. It is primarily expressed on monocytes, macrophages, and neutrophils, and soluble forms can be secreted or shed from the cell surface. CD14 accepts LPS from its initial binding by LBP, and it can further transfer LPS to the TLR4/MD2 complex. LPS acctivation of TLR4 triggers inflammatory activation. CD14 also contributes to the response to TLR3 ligands, phosphoinositides, as well as the surfactant proteins SP-A and SP-D.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Publications for CD14 (DC140)(85)

We have publications tested in 6 confirmed species: Human, Primate, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Primate - Macaca nemestrina (Southern Pig-tailed Macaque).

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Primate - Chlorocebus pygerythrus (Vervet Monkey)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Primate - Macaca mulatta (Rhesus Macaque)
Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
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Showing Publications 1 - 10 of 85. Show All 85 Publications.
Publications using DC140 Applications Species
JF Camargo, S Pallikkuth, I Moroz, Y Natori, ML Alcaide, A Rodriguez, G Guerra, GW Burke, S Pahwa Pretransplant Levels of C-Reactive Protein,�Soluble TNF Receptor-1, and�CD38+HLADR+ CD8 T Cells Predict Risk of Allograft Rejection in HIV+ Kidney Transplant Recipients Kidney Int Rep, 2019;4(12):1705-1716. 2019 [PMID: 31844807] (Human) Human
JA Manuzak, AS Zevin, R Cheu, B Richardson, J Modesitt, T Hensley-Mc, C Miller, AT Gustin, E Coronado, T Gott, M Fang, M Cartwright, S Wangari, B Agricola, D May, E Smith, HB Hampel, M Gale, CM Cameron, MJ Cameron, J Smedley, NR Klatt Antibiotic-induced microbiome perturbations are associated with significant alterations to colonic mucosal immunity in rhesus macaques Mucosal Immunol, 2019;0(0):. 2019 [PMID: 31797911] (Human) Human
JR Chu, SY Kang, SE Kim, SJ Lee, YC Lee, MK Sung Prebiotic UG1601 mitigates constipation-related events in association with gut microbiota: A randomized placebo-controlled intervention study World J. Gastroenterol., 2019;25(40):6129-6144. 2019 [PMID: 31686768] (Human) Human
N Aziz, R Detels, JJ Quint, D Gjertson, T Ryner, AW Butch Biological variation of immunological blood biomarkers in healthy individuals and quality goals for biomarker tests BMC Immunol., 2019;20(1):33. 2019 [PMID: 31521107] (Human) Human
E Barengolts, SJ Green, GE Chlipala, BT Layden, Y Eisenberg, M Priyadarsh, LR Dugas Predictors of Obesity among Gut Microbiota Biomarkers in African American Men with and without Diabetes Microorganisms, 2019;7(9):. 2019 [PMID: 31491976] (Human) Human
DT Claiborne, EP Scully, CD Palmer, JL Prince, GN Macharia, J Kopycinski, CM Michelo, HW Wiener, R Parker, K Nganou-Mak, D Douek, M Altfeld, J Gilmour, MA Price, J Tang, W Kilembe, SA Allen, E Hunter Protective HLA alleles are associated with reduced LPS levels in acute HIV infection with implications for immune activation and pathogenesis PLoS Pathog., 2019;15(8):e1007981. 2019 [PMID: 31449552] (Human) Human
MS Moon, G Quinn, EC Townsend, RO Ali, GY Zhang, A Bradshaw, K Hill, H Guan, D Hamilton, DE Kleiner, C Koh, T Heller Bacterial Translocation and Host Immune Activation in Chronic Hepatitis C Infection Open Forum Infect Dis, 2019;6(7):. 2019 [PMID: 31363763] (Human) Human
MS Moon, G Quinn, EC Townsend, RO Ali, GY Zhang, A Bradshaw, K Hill, H Guan, D Hamilton, DE Kleiner, C Koh, T Heller Bacterial Translocation and Host Immune Activation in Chronic Hepatitis C Infection Open Forum Infect Dis, 2019;6(7):. 2019 [PMID: 31363763] (Human) Human
C Missailidi, N Sørensen, S Ashenafi, W Amogne, E Kassa, A Bekele, M Getachew, N Gebreselas, A Aseffa, G Aderaye, J Andersson, S Brighenti, P Bergman Vitamin D and Phenylbutyrate Supplementation Does Not Modulate Gut Derived Immune Activation in HIV-1 Nutrients, 2019;11(7):. 2019 [PMID: 31330899] (Human) Human
M Masiá, S Padilla, JA García, J García-Abe, M Fernández, I Bernardino, M Montero, J Peraire, B Pernas, F Gutiérrez Evolving understanding of cardiovascular, cerebrovascular and peripheral arterial disease in people living with HIV and role of novel biomarkers. A study of the Spanish CoRIS cohort, 2004-2015 PLoS ONE, 2019;14(4):e0215507. 2019 [PMID: 31026289] (Human) Human
Show All 85 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD14 (DC140). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

CD14 - TLR4 is my friend in battle against infections!
CD14 is a well-characterized cell-activating receptor for lipopolysaccharide-binding protein (LBP) and peptidoglycan. It is an important modulator for lipopolysaccharide (LPS)-dependent signaling and is a component of the multi-protein complex contain...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

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