Human CD14 Quantikine ELISA Kit



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Reactivity HuSpecies Glossary
Applications ELISA

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Human CD14 Quantikine ELISA Kit Summary

The Quantikine Human soluble CD14 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human soluble CD14 in cell culture supernates, serum, and plasma. It contains recombinant human CD14 expressed from CHO cells and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained measuring natural ...human sCD14 showed dose-response curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human sCD14.
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Natural and recombinant human sCD14
Assay Type
Solid Phase Sandwich ELISA
See PDF Datasheet for details
See PDF Datasheet for details
Spike Recovery
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Sample Volume
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Application Notes
No significant interference observed with available related molecules.
Read Publications using DC140.

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CD14 Quantikine ELISA Kit

  • CD14 antigen
  • CD14 molecule
  • CD14
  • monocyte differentiation antigen CD14
  • Myeloid cell-specific leucine-rich glycoprotein


CD14 is a glycoprotein that mediates the interaction of lipopolysaccharide (LPS, endotoxin)with cells, thereby signaling the presence of gram-negative bacteria (1-3). CD14 is eithersoluble (CD14) (4, 5) or membrane-bound (mCD14) by a glycosylphosphatidylinositol (GPI)anchor (6, 7). mCD14 is a 55 kDa glycoprotein (1), while CD14 varies from about 43 to 53 kDa,depending on the degree of glycosylation and whether it was synthesized without the anchoror was shed by phospholipase cleavage of the anchor or by proteolysis (12-14). There is noevidence for different mRNAs for m- and CD14. There is no apparent sequence homology withother proteins. The sequence of human CD14 is 63-73% identical to that of mouse, rat, or rabbitCD14 (15).
mCD14 is expressed primarily on myeloid cells, such as monocytes, macrophages andneutrophils (1-3), the cells most sensitive to LPS, and to a lesser extent on other cells, such as Bcells (8) and a circulating dendritic cell progenitor (9). CD14 appears to mediate LPS stimulationof cells that do not express mCD14 (10, 11), such as endothelial, epithelial and smooth-musclecells. CD14 is found in both serum and urine (5).
The binding of LPS to CD14 requires an acute phase protein, LPS-binding protein (LBP) (16).The relationship of mCD14, CD14, LPS and LBP is complicated. At low concentrations of LPS,LBP is essential for the binding of LPS to CD14, but at high concentrations, LBP may actuallyinhibit binding of LPS to CD14. In addition, CD14 may compete with mCD14 for LPS (17) andmay serve to help clear LPS (18). These four factors thus appear to participate in a complexfeedback mechanism of immune regulation involving both up-regulation and down-regulationof the inflammatory process triggered by LPS. It is loss of control of this mechanism thatappears to lead to septic shock. LPS-bound CD14 signals production of inflammatory cytokinesand other inflammatory proteins, but the mechanism of signal transduction is unclear. Sincea GPI anchor is not transmembrane, there presumably is another transmembrane protein oncells through which LPS-bound CD14 transmits a signal (19).
In addition to its well known role in gram-negative infections, CD14 likely serves otherfunctions as well. It recognizes soluble peptidylglycan from gram-positive cell walls (20), and ithas been reported to bind apoptotic cells and induce their phagocytosis (21).

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Publications for CD14 (DC140)(123)

We have publications tested in 6 confirmed species: Human, Primate, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Primate - Macaca nemestrina (Southern Pig-tailed Macaque).

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Primate - Chlorocebus pygerythrus (Vervet Monkey)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Primate - Macaca mulatta (Rhesus Macaque)
Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
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Showing Publications 1 - 10 of 123. Show All 123 Publications.
Publications using DC140 Applications Species
P Zuccalà, T Latronico, R Marocco, S Savinelli, S Vita, F Mengoni, T Tieghi, C Borgo, B Kertusha, A Carraro, G D'Ettorre, V Vullo, CM Mastroiann, GM Liuzzi, M Lichtner Longitudinal Assessment of Multiple Immunological and Inflammatory Parameters during Successful DAA Therapy in HCV Monoinfected and HIV/HCV Coinfected Subjects International Journal of Molecular Sciences, 2022;23(19):. 2022 [PMID: 36233234] (Human) Human
TE Peterson, C Landon, SA Haberlen, F Bhondoekha, MW Plankey, FJ Palella, DA Piggott, JB Margolick, TT Brown, WS Post, KC Wu Circulating biomarker correlates of left atrial size and myocardial extracellular volume fraction among persons living with and without HIV BMC cardiovascular disorders, 2022;22(1):393. 2022 [PMID: 36057773] (Human) Human
A Merino-Rib, R Araujo, L Pereira, J Campos, L Barreiros, MA Segundo, N Silva, CFFA Costa, J Quelhas-Sa, F Trindade, I Falcão-Pir, I Alencastre, IB Dumitrescu, B Sampaio-Ma Vascular Calcification and the Gut and Blood Microbiome in Chronic Kidney Disease Patients on Peritoneal Dialysis: A Pilot Study Biomolecules, 2022;12(7):. 2022 [PMID: 35883423] (Human) Human
Z Strongin, TN Hoang, GK Tharp, AR Rahmberg, JL Harper, K Nguyen, L Franchitti, B Cervasi, M Lee, Z Zhang, EA Boritz, G Silvestri, VC Marconi, SE Bosinger, JM Brenchley, DA Kulpa, M Paiardini The role of CD101-expressing CD4 T cells in HIV/SIV pathogenesis and persistence PloS Pathogens, 2022;18(7):e1010723. 2022 [PMID: 35867722] (Human) Human
A Kholodnaia, K So-Armah, D Cheng, N Gnatienko, G Patts, JH Samet, M Freiberg, D Lioznov Impact of illicit opioid use on markers of monocyte activation and systemic inflammation in people living with HIV PLoS ONE, 2022;17(5):e0265504. 2022 [PMID: 35511802] (Human) Human
C Amadieu, L Maccioni, S Leclercq, AM Neyrinck, NM Delzenne, P de Timary, P Stärkel Liver alterations are not improved by inulin supplementation in alcohol use disorder patients during alcohol withdrawal: A pilot randomized, double-blind, placebo-controlled study EBioMedicine, 2022;80(0):104033. 2022 [PMID: 35490461] (Human) Human
J Štšepetova, K Simre, A Tagoma, O Uibo, A Peet, H Siljander, V Tillmann, M Knip, R Mändar, R Uibo Maternal breast milk microbiota and immune markers in relation to subsequent development of celiac disease in offspring Scientific Reports, 2022;12(1):6607. 2022 [PMID: 35459889] (Human) Human
L Hernandez, LJ Ward, S Arefin, T Ebert, A Laucyte-Ci, GOING-FWD, O Heimbürger, P Barany, L Wennberg, P Stenvinkel, K Kublickien Blood-brain barrier and gut barrier dysfunction in chronic kidney disease with a focus on circulating biomarkers and tight junction proteins Scientific Reports, 2022;12(1):4414. 2022 [PMID: 35292710] (Human) Human
T Sainz, L Diaz, D Rojo, MI Clemente, C Barbas, MJ Gosalbes, N Jimenez-He, L Escosa, S Guillen, JT Ramos, MÁ Muñoz-Fern, ML Navarro, MJ Mellado, S Serrano-Vi Targeting the Gut Microbiota of Vertically HIV-Infected Children to Decrease Inflammation and Immunoactivation: A Pilot Clinical Trial Nutrients, 2022;14(5):. 2022 [PMID: 35267967] (Human) Human
W Marx, S Thomson, M O'Hely, C Symeonides, F Collier, MLK Tang, A Loughman, D Burgner, R Saffery, C Pham, T Mansell, PD Sly, P Vuillermin, S Ranganatha, AL Ponsonby, Barwon Inf Maternal inflammatory and omega-3 fatty acid pathways mediate the association between socioeconomic disadvantage and childhood cognition Brain, Behavior, and Immunity, 2021;100(0):211-218. 2021 [PMID: 34896180] (Human) Human
Show All 123 Publications.

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The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

CD14 - TLR4 is my friend in battle against infections!
CD14 is a well-characterized cell-activating receptor for lipopolysaccharide-binding protein (LBP) and peptidoglycan. It is an important modulator for lipopolysaccharide (LPS)-dependent signaling and is a component of the multi-protein complex contain...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
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