Human CD14 Quantikine ELISA Kit

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

Human CD14 Quantikine ELISA Kit Summary

Background
The Quantikine Human soluble CD14 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human soluble CD14 in cell culture supernates, serum, and plasma. It contains recombinant human CD14 expressed from CHO cells and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained measuring natural ...human sCD14 showed dose-response curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human sCD14.
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Specificity
Natural and recombinant human sCD14
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
CD14

Applications/Dilutions

Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DC140.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CD14 Quantikine ELISA Kit

  • CD14 antigen
  • CD14 molecule
  • CD14
  • monocyte differentiation antigen CD14
  • Myeloid cell-specific leucine-rich glycoprotein

Background

CD14 is a glycoprotein that mediates the interaction of lipopolysaccharide (LPS, endotoxin)with cells, thereby signaling the presence of gram-negative bacteria (1-3). CD14 is eithersoluble (CD14) (4, 5) or membrane-bound (mCD14) by a glycosylphosphatidylinositol (GPI)anchor (6, 7). mCD14 is a 55 kDa glycoprotein (1), while CD14 varies from about 43 to 53 kDa,depending on the degree of glycosylation and whether it was synthesized without the anchoror was shed by phospholipase cleavage of the anchor or by proteolysis (12-14). There is noevidence for different mRNAs for m- and CD14. There is no apparent sequence homology withother proteins. The sequence of human CD14 is 63-73% identical to that of mouse, rat, or rabbitCD14 (15).
mCD14 is expressed primarily on myeloid cells, such as monocytes, macrophages andneutrophils (1-3), the cells most sensitive to LPS, and to a lesser extent on other cells, such as Bcells (8) and a circulating dendritic cell progenitor (9). CD14 appears to mediate LPS stimulationof cells that do not express mCD14 (10, 11), such as endothelial, epithelial and smooth-musclecells. CD14 is found in both serum and urine (5).
The binding of LPS to CD14 requires an acute phase protein, LPS-binding protein (LBP) (16).The relationship of mCD14, CD14, LPS and LBP is complicated. At low concentrations of LPS,LBP is essential for the binding of LPS to CD14, but at high concentrations, LBP may actuallyinhibit binding of LPS to CD14. In addition, CD14 may compete with mCD14 for LPS (17) andmay serve to help clear LPS (18). These four factors thus appear to participate in a complexfeedback mechanism of immune regulation involving both up-regulation and down-regulationof the inflammatory process triggered by LPS. It is loss of control of this mechanism thatappears to lead to septic shock. LPS-bound CD14 signals production of inflammatory cytokinesand other inflammatory proteins, but the mechanism of signal transduction is unclear. Sincea GPI anchor is not transmembrane, there presumably is another transmembrane protein oncells through which LPS-bound CD14 transmits a signal (19).
In addition to its well known role in gram-negative infections, CD14 likely serves otherfunctions as well. It recognizes soluble peptidylglycan from gram-positive cell walls (20), and ithas been reported to bind apoptotic cells and induce their phagocytosis (21).

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CD14 (DC140)(86)

We have publications tested in 6 confirmed species: Human, Primate, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Primate - Macaca nemestrina (Southern Pig-tailed Macaque).


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Human
(77)
Primate
(1)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(2)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(3)
Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
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Showing Publications 1 - 10 of 86. Show All 86 Publications.
Publications using DC140 Applications Species
CL Vinton, CE Starke, AM Ortiz, SH Lai, JK Flynn, O Sortino, K Knox, I Sereti, JM Brenchley Biomarkers of Cellular Stress Do Not Associate with sCD14 in Progressive HIV and SIV Infections in Vivo Pathog Immun, 2020;5(1):68-88. 2020 [PMID: 32426577] (Human) Human
JF Camargo, S Pallikkuth, I Moroz, Y Natori, ML Alcaide, A Rodriguez, G Guerra, GW Burke, S Pahwa Pretransplant Levels of C-Reactive Protein,�Soluble TNF Receptor-1, and�CD38+HLADR+ CD8 T Cells Predict Risk of Allograft Rejection in HIV+ Kidney Transplant Recipients Kidney Int Rep, 2019;4(12):1705-1716. 2019 [PMID: 31844807] (Human) Human
JA Manuzak, AS Zevin, R Cheu, B Richardson, J Modesitt, T Hensley-Mc, C Miller, AT Gustin, E Coronado, T Gott, M Fang, M Cartwright, S Wangari, B Agricola, D May, E Smith, HB Hampel, M Gale, CM Cameron, MJ Cameron, J Smedley, NR Klatt Antibiotic-induced microbiome perturbations are associated with significant alterations to colonic mucosal immunity in rhesus macaques Mucosal Immunol, 2019;0(0):. 2019 [PMID: 31797911] (Human) Human
JR Chu, SY Kang, SE Kim, SJ Lee, YC Lee, MK Sung Prebiotic UG1601 mitigates constipation-related events in association with gut microbiota: A randomized placebo-controlled intervention study World J. Gastroenterol., 2019;25(40):6129-6144. 2019 [PMID: 31686768] (Human) Human
N Aziz, R Detels, JJ Quint, D Gjertson, T Ryner, AW Butch Biological variation of immunological blood biomarkers in healthy individuals and quality goals for biomarker tests BMC Immunol., 2019;20(1):33. 2019 [PMID: 31521107] (Human) Human
E Barengolts, SJ Green, GE Chlipala, BT Layden, Y Eisenberg, M Priyadarsh, LR Dugas Predictors of Obesity among Gut Microbiota Biomarkers in African American Men with and without Diabetes Microorganisms, 2019;7(9):. 2019 [PMID: 31491976] (Human) Human
DT Claiborne, EP Scully, CD Palmer, JL Prince, GN Macharia, J Kopycinski, CM Michelo, HW Wiener, R Parker, K Nganou-Mak, D Douek, M Altfeld, J Gilmour, MA Price, J Tang, W Kilembe, SA Allen, E Hunter Protective HLA alleles are associated with reduced LPS levels in acute HIV infection with implications for immune activation and pathogenesis PLoS Pathog., 2019;15(8):e1007981. 2019 [PMID: 31449552] (Human) Human
MS Moon, G Quinn, EC Townsend, RO Ali, GY Zhang, A Bradshaw, K Hill, H Guan, D Hamilton, DE Kleiner, C Koh, T Heller Bacterial Translocation and Host Immune Activation in Chronic Hepatitis C Infection Open Forum Infect Dis, 2019;6(7):. 2019 [PMID: 31363763] (Human) Human
MS Moon, G Quinn, EC Townsend, RO Ali, GY Zhang, A Bradshaw, K Hill, H Guan, D Hamilton, DE Kleiner, C Koh, T Heller Bacterial Translocation and Host Immune Activation in Chronic Hepatitis C Infection Open Forum Infect Dis, 2019;6(7):. 2019 [PMID: 31363763] (Human) Human
C Missailidi, N Sørensen, S Ashenafi, W Amogne, E Kassa, A Bekele, M Getachew, N Gebreselas, A Aseffa, G Aderaye, J Andersson, S Brighenti, P Bergman Vitamin D and Phenylbutyrate Supplementation Does Not Modulate Gut Derived Immune Activation in HIV-1 Nutrients, 2019;11(7):. 2019 [PMID: 31330899] (Human) Human
Show All 86 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD14 (DC140). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

HRP DC140

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Blogs on CD14.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

CD14 - TLR4 is my friend in battle against infections!
CD14 is a well-characterized cell-activating receptor for lipopolysaccharide-binding protein (LBP) and peptidoglycan. It is an important modulator for lipopolysaccharide (LPS)-dependent signaling and is a component of the multi-protein complex contain...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

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Bioinformatics

Gene Symbol CD14