TLR3 Antibody (40C1285.6)


Flow Cytometry: TLR3 Antibody (40C1285.6) [NBP2-24875] - Expression of TLR3 protein on epithelial cells. HNEC (A), Detroit-562 (B) and FaDu (C) were stained intracellularly with FITC-Abs against TLR3 (open histograms) more
Western Blot: TLR3 Antibody (40C1285.6) [NBP2-24875] - All the cell lines displayed a strong expression of TLR3 protein. Cell lysates were electrophoresed and blotted to PVDF membrane, which was probed with more
Flow Cytometry: TLR3 Antibody (40C1285.6) [NBP2-24875] - Expression of TLR3 in Fravel, L363, OPM1, RPMI8226, XG1, and NCI-H929 as determined by flow cytometry. HCMLs were stained using an intracellular staining protocol more
Immunohistochemistry-Paraffin: TLR3 Antibody (40C1285.6) [NBP2-24875] - Mouse colon using TLR3 antibody (clone 40C1285.6) at 1:500 dilution with HRP-DAB detection and hematoxylin counterstaining. Intense signal was more
Western Blot: TLR3 Antibody (40C1285.6) [NBP2-24875] - Analysis of TLR3 in lysates from A) human intestine and F) ovary using TLR3 antibody at 3 ug/ml. Goat anti-mouse HRP conjugate was used as secondary.
Western Blot: TLR3 Antibody (40C1285.6) [NBP2-24875] - HEK293T cells were transfected with TLR3, TLR3-9-3 and TLR3-3-9. Western blot was performed using anti-TLR3 or anti-HA antibodies. Anti-Beta-actin antibodies were more
Immunohistochemistry-Paraffin: TLR3 Antibody (40C1285.6) [NBP2-24875] - Tissue section of mouse intestine using TLR3 antibody (clone 40C1285.6) at 1:500 dilution with HRP-DAB detection and hematoxylin counterstaining. more
Immunohistochemistry-Paraffin: TLR3 Antibody (40C1285.6) [NBP2-24875] - Analysis of TLR3 in (A) human gut lumen (longitudinal section, transverse region) using this antibody at 10 ug/ml (Data courtesy Dr. Elizabeth more

Product Details

Reactivity Hu, Mu, CaSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P, IP, B/N, Bioactivity
1.0 mg/ml

Order Details

TLR3 Antibody (40C1285.6) Summary

A synthetic peptide corresponding to amino acids 55-85 of human TLR3 was used as immunogen.
IgG1 Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1-3 ug/ml
  • Flow Cytometry 2-4 ug/ 1x10^6 cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2 ug/ 1x10^6 cells
  • Bioactivity
  • Block/Neutralize
  • Flow (Intracellular)
Application Notes
Use in Flow-intracellular reported in scientific literature (PMID: 24836676). Use in blocking/ neutralizing reported in scientific literature (PMID: 28894449). Use in Bioactivity reported in scientific literature (PMID:28894449).
Read Publications using
NBP2-24875 in the following applications:

  • BA
    1 publication
  • 2 publications
  • 5 publications
  • 1 publication
  • 10 publications
  • IHC
    4 publications
  • 5 publications
  • IP
    3 publications
  • WB
    22 publications

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for TLR3 Antibody (40C1285.6)

  • CD283 antigen
  • CD283
  • TLR3
  • toll-like receptor 3


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Po, Bv, Ma
Applications: WB, ChIP, DB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, PAGE, B/N, CyTOF-ready, Dual ISH-IHC, ELISA(Cap), Flow-CS, Flow-IC, KD, KO
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, DB, ELISA, Flow, Func, ICC/IF, IP, In vitro, B/N, Flow-CS
Species: Hu, Mu, Rt, Ca, Eq, Pm, Pm
Applications: WB, Simple Western, DB, ELISA, Flow, Func, ICC/IF, IHC, IHC-P, IP, In vitro, B/N, CyTOF-ready, Flow-IC, KD
Species: Hu, Mu, Rt
Applications: WB, DB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, BA, Flow-IC
Species: Mu, Rt, Po
Applications: WB, Simple Western, IB, ICC/IF, IHC, IHC-P, IP, KD
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
Species: Hu, Pm
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Ca
Applications: WB, DB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-CS, Flow-IC
Species: Hu, Mu, Rt, Bv, Xp, Ye, Ze
Applications: ELISA
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rb
Applications: WB, Simple Western, DB, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, Flow-CS, Flow-IC
Species: Hu, Mu
Applications: WB, IHC, IHC-P, PEP-ELISA
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Pm
Applications: WB, IHC, IHC-P, PEP-ELISA
Species: Hu, Mu, Rt
Applications: WB, DB, Flow, IHC, IHC-P, Flow-CS, Flow-IC
Species: Hu
Applications: WB, Simple Western, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
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Publications for TLR3 Antibody (NBP2-24875)(78)

We have publications tested in 3 confirmed species: Human, Mouse, Canine.

We have publications tested in 9 applications: BA, FLOW, Flow, Flow-IC, ICC/IF, IHC, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 78. Show All 78 Publications.
Publications using NBP2-24875 Applications Species
Wolpaw A, Grossmann L, Dong M, et al. Epigenetic state determines inflammatory sensing in neuroblastoma bioRxiv Jan 28 2021 (WB, Mouse) WB Mouse
Li Y, Chen X, Jin R et al. Injectable hydrogel with MSNs/microRNA-21-5p delivery enables both immunomodification and enhanced angiogenesis for myocardial infarction therapy in pigs Science advances Feb 4 2021 [PMID: 33627421] (WB, Mouse) WB Mouse
Yoshida T, Miura T, Matsumiya T et al. Toll-Like Receptor 3 as a Recurrence Risk Factor and a Potential Molecular Therapeutic Target in Colorectal Cancer Clin Exp Gastroenterol Oct 2 2020 [PMID: 33061521] (IHC-P, Human) IHC-P Human
Shang L, Deng D, Roffel S, Gibbs S Differential influence of Streptococcus mitis on host response to metals in reconstructed human skin and oral mucosa Contact Derm. Jul 16 2020 [PMID: 32677222] (WB, Human) WB Human
Helminen O, Huhta H, Lehenkari Petri P. Nucleic acid-sensing Toll-like receptors 3, 7 and 8 in esophageal epithelium, Barrett's esophagus, dysplasia and adenocarcinoma OncoImmunology Jan 25 2016
Igase M, Shousu K, Fujiki N et al. Anti-tumour activity of oncolytic reovirus against canine histiocytic sarcoma cells Vet Comp Oncol Feb 14 2019 [PMID: 30761736] (WB, Canine) WB Canine
Gao X, Gao S, Guan Y et al. Toll-like receptor 3 controls QT interval on the electrocardiogram by targeting the degradation of Kv4.2/4.3 channels in the endoplasmic reticulum FASEB J. Feb 13 2019 [PMID: 30758987] (WB, Human) WB Human
Bauer JMJ. Interferon-b moduliert die Expression von Toll-like Rezeptoren in mononuklearen Blutzellen und induziert Toll-like Rezeptor 7 in plasmazytoiden dendritischen Zellen von Patienten mit Multipler Sklerose. thesis. 2015 Nov 12
Regel I, Raulefs S, Benitz S et al. Loss of TLR3 and its downstream signaling accelerates acinar cell damage in the acute phase of pancreatitis Pancreatology Jan 1 2019 [PMID: 30583980] (IHC, Mouse) IHC Mouse
Zhu J, Message SD, Mallia P et al. Bronchial mucosal Interferon-A/B and pattern recognition receptor expression in experimental rhinovirus-induced asthma exacerbations. J. Allergy Clin. Immunol. Apr 23 2018 [PMID: 29698627] (IHC, Human) IHC Human
Show All 78 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-24875AF350
Alexa Fluor 405 NBP2-24875AF405
Alexa Fluor 488 NBP2-24875AF488
Alexa Fluor 532 NBP2-24875AF532
Alexa Fluor 594 NBP2-24875AF594
Alexa Fluor 647 NBP2-24875AF647
Alexa Fluor 700 NBP2-24875AF700
Alexa Fluor 750 NBP2-24875AF750
Allophycocyanin NBP2-24875APC
Biotin NBP2-24782
DyLight 350 NBP2-24875UV
DyLight 405 NBP2-24875V
DyLight 488 NBP2-24875G
DyLight 550 NBP2-24875R
DyLight 594 NBP2-24875DL594
DyLight 650 NBP2-24875C
DyLight 680 NBP2-24875FR
DyLight 755 NBP2-24875IR
FITC NBP2-24875F
HRP NBP2-24875H
Janelia Fluor 549 NBP2-24875JF549
Janelia Fluor 646 NBP2-24875JF646
PE NBP2-24902
PerCP NBP2-24875PCP

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The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

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Gene Symbol TLR3