TLR3 Antibody (40C1285.6)

Images

 
Western Blot: TLR3 Antibody (40C1285.6) [NBP2-24875] - Analysis of TLR3 in lysates from A) untransfected 293, B) 293 cells transfected with human TLR3 cDNA, C) human intestine, D) placenta, E) heart and F) ovary using ...read more
Immunohistochemistry-Paraffin: TLR3 Antibody (40C1285.6) [NBP2-24875] - Tissue section of mouse intestine using TLR3 antibody (clone 40C1285.6) at 1:500 dilution with HRP-DAB detection and hematoxylin counterstaining. ...read more
Flow (Intracellular): TLR3 Antibody (40C1285.6) [NBP2-24875] - Analysis using the PE conjugate of NBP2-24875. Staining of TLR3 in human monocytes using 0.5 ug/10^6 cells of this antibody. Shaded histogram represents ...read more
Immunohistochemistry-Paraffin: TLR3 Antibody (40C1285.6) [NBP2-24875] - Analysis of TLR3 in (A) human gut lumen (longitudinal section, transverse region) using this antibody at 10 ug/ml (Data courtesy Dr. Elizabeth ...read more
Immunohistochemistry-Paraffin: TLR3 Antibody (40C1285.6) [NBP2-24875] - Mouse colon using TLR3 antibody (clone 40C1285.6) at 1:500 dilution with HRP-DAB detection and hematoxylin counterstaining. Intense signal was ...read more
Flow Cytometry: TLR3 Antibody (40C1285.6) [NBP2-24875] - Intracellular flow analysis of TLR3 in human monocytes using 0.5 ug of the antibody. Shaded histogram represents monocytes without antibody; green represents ...read more
Flow Cytometry: TLR3 Antibody (40C1285.6) [NBP2-24875] - Analysis using the FITC conjugate of NBP2-24875. Staining of TLR3 in human B cells using 1ug/10^6 cells of this antibody. Green represents isotype control; red ...read more

Product Details

Summary
Reactivity Hu, Mu, CaSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P, IP
Clone
40C1285.6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

TLR3 Antibody (40C1285.6) Summary

Immunogen
A synthetic peptide corresponding to amino acids 55-85 of human TLR3 was used as immunogen.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TLR3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Flow Cytometry 2-4 ug/ 1x10^6 cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2 ug/ 1x10^6 cells
  • Flow (Intracellular)
Application Notes
Use in Flow-intracellular reported in scientific literature (PMID: 24836676).
Agonist
Publications
Read Publications using
NBP2-24875 in the following applications:

  • BA
    1 publication
  • 5 publications
  • 5 publications
  • 1 publication
  • 10 publications
  • IHC
    4 publications
  • 4 publications
  • IP
    3 publications
  • WB
    19 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TLR3 Antibody (40C1285.6)

  • CD283 antigen
  • CD283
  • TLR3
  • toll-like receptor 3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for TLR3 Antibody (NBP2-24875)(77)

We have publications tested in 3 confirmed species: Human, Mouse, Canine.

We have publications tested in 9 applications: BA, FLOW, Flow, Flow-IC, ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
BA
(1)
FLOW
(5)
Flow
(5)
Flow-IC
(1)
ICC/IF
(10)
IHC
(4)
IHC-P
(4)
IP
(3)
WB
(19)
All Applications
Filter By Species
Human
(27)
Mouse
(10)
Canine
(1)
All Species
Showing Publications 1 - 10 of 77. Show All 77 Publications.
Publications using NBP2-24875 Applications Species
Igase M, Shousu K, Fujiki N et al. Anti-tumour activity of oncolytic reovirus against canine histiocytic sarcoma cells Vet Comp Oncol Feb 14 2019 [PMID: 30761736] (WB, Canine) WB Canine
Gao X, Gao S, Guan Y et al. Toll-like receptor 3 controls QT interval on the electrocardiogram by targeting the degradation of Kv4.2/4.3 channels in the endoplasmic reticulum FASEB J. Feb 13 2019 [PMID: 30758987] (WB, Human) WB Human
Regel I, Raulefs S, Benitz S et al. Loss of TLR3 and its downstream signaling accelerates acinar cell damage in the acute phase of pancreatitis Pancreatology Jan 1 2019 [PMID: 30583980] (IHC, Mouse) IHC Mouse
Zhu J, Message SD, Mallia P et al. Bronchial mucosal Interferon-A/B and pattern recognition receptor expression in experimental rhinovirus-induced asthma exacerbations. J. Allergy Clin. Immunol. Apr 23 2018 [PMID: 29698627] (IHC, Human) IHC Human
Gu R, Ding M, Shi D et al. Calcium/Calmodulin-Dependent Protein Kinase IV Mediates IFN-gamma-Induced Immune Behaviors in Skeletal Muscle Cells Cell. Physiol. Biochem. 2018 Mar 22 [PMID: 29590648] (WB, Mouse) WB Mouse
Zou Haitao, Su Ruixue, Ruan Jing et al. Toll-like receptor 3 pathway restricts Marek's disease virus infection. Oncotarget 2017 [PMID: 29050325] (WB, Human) WB Human
Bauer JMJ. Interferon-b moduliert die Expression von Toll-like Rezeptoren in mononuklearen Blutzellen und induziert Toll-like Rezeptor 7 in plasmazytoiden dendritischen Zellen von Patienten mit Multipler Sklerose. thesis. 2015 Nov 12
Tengroth L, Arebro J, Kumlien Georen S et al. Deprived TLR9 Expression in Apparently Healthy Nasal Mucosa Might Trigger Polyp-Growth in Chronic Rhinosinusitis Patients PLoS ONE (FLOW, Human)

Details:
This publication used the PE conjugated form of this antibody (Cat# NBP2-24902).
FLOW Human
Green TL, Santos MF, Ejaeidi AA et al. Toll-like receptor (TLR) expression of immune system cells from metastatic breast cancer patients with circulating tumor cells Exp Mol Pathol (FLOW, Human)

Details:
This publication used the PE conjugated form of this antibody (Cat# NBP2-24902).
FLOW Human
Jamin A, Dehoux L, Dossier C et al. Toll-like receptor 3 expression and function in childhood idiopathic nephrotic syndrome Clin Exp Immunol (FLOW, Human)

Details:
This publication used the PE conjugated form of this antibody (Cat# NBP2-24902).
FLOW Human
Show All 77 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TLR3 Antibody (NBP2-24875) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-24875AF350
Alexa Fluor 405 NBP2-24875AF405
Alexa Fluor 488 NBP2-24875AF488
Alexa Fluor 532 NBP2-24875AF532
Alexa Fluor 594 NBP2-24875AF594
Alexa Fluor 647 NBP2-24875AF647
Alexa Fluor 700 NBP2-24875AF700
Alexa Fluor 750 NBP2-24875AF750
Allophycocyanin NBP2-24875APC
Allophycocyanin/Cy7 NBP2-24875APCCY7
Biotin NBP2-24782
DyLight 350 NBP2-24875UV
DyLight 405 NBP2-24875V
DyLight 488 NBP2-24875G
DyLight 550 NBP2-24875R
DyLight 594 NBP2-24875DL594
DyLight 650 NBP2-24875C
DyLight 680 NBP2-24875FR
DyLight 755 NBP2-24875IR
FITC NBP2-24875F
HRP NBP2-24875H
Janelia Fluor 549 NBP2-24875JF549
Janelia Fluor 646 NBP2-24875JF646
PE NBP2-24902
PE/Atto594 NBP2-24875PEATT594
PE/Cy5.5 NBP2-24875PECY55
PE/Cy7 NBP2-24875PECY7
PerCP NBP2-24875PCP

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Bioinformatics Tool for TLR3 Antibody (NBP2-24875)

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Blogs on TLR3.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

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Bioinformatics

Gene Symbol TLR3