NOD2 Antibody (2D9) - BSA Free


Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs more
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
Flow (Intracellular): NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on THP-1 cells with NOD2 (2D9) antibody NB100-524AF488 (blue) and a matched isotype control NBP2-27287AF488 (orange). Cells more
Western Blot: NOD2 Antibody (2D9) - BSA Free [NB100-524]
Immunocytochemistry/ Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - NOD2 was detected in human monocytes using NOD2 antibody (2D9) [DyLight 488 (NB100-524G)]- with a concentration of 1:500 in PBS for 2 hours. more
Immunohistochemistry: NOD2 Antibody (2D9) [NB100-524] - Analysis of PFA fixed mouse sciatic nerve section using anti-NOD2 antibody. Image from verified customer review.
Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on THP-1 cells with NOD2 Antibody (2D9) NB100-524 (blue) and a matched mouse IgG1 isotype control (orange) MAB002. Cells were fixed more
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Whole cell protein from THP-1 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed more
Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA more
Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and more

Product Details

Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP
BSA Free
1.0 mg/ml

Order Details

NOD2 Antibody (2D9) - BSA Free Summary

Recombinant human NOD2 protein corresponding to residues 28-301. [UniProt# Q9HC29]
Predicted Species
Primate (92%). Backed by our 100% Guarantee.
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow (Intracellular)
  • Flow Cytometry reported in scientific literature (PMID 28241127)
  • Immunocytochemistry/Immunofluorescence 1:50-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
In WB, this antibody has been tested with NOD2 transfected 293T lysates and HT29 human colorectal adenocarcinoma lysates (endogenous detection has some background) where a band is seen at ~110 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
NB100-524 in the following applications:

Read Publications using
NB100-524 in the following applications:

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for NOD2 Antibody (2D9) - BSA Free

  • BLAU
  • CARD15caspase recruitment domain protein 15
  • caspase recruitment domain family, member 15
  • Caspase recruitment domain-containing protein 15
  • CLR16.3
  • IBD1NLR family, CARD domain containing 2
  • Inflammatory bowel disease protein 1
  • NLRC2
  • NOD-like receptor C2
  • nucleotide-binding oligomerization domain 2
  • nucleotide-binding oligomerization domain containing 2
  • nucleotide-binding oligomerization domain, leucine rich repeat and CARD domaincontaining 2
  • nucleotide-binding oligomerization domain-containing protein 2


The NOD2 (nucleotide-binding oligomerization domain containing 2) protein is expressed in the antigen-presenting cells (monocytes and macrophages) and in intestinal Paneth cells, keratinocytes, T cells, NK cells, and CD34+ stem cells. Alongwith Apaf-1 and CIITA, NOD2 belongs to NLR/NOD leucine-rich repeat containing protein family. NOD2 generally localizes to the cytoplasm from where it translocates to cell membranes, a mechanism suggested as important for its functionality. After interacting with CARD-CARD, NOD2 may bind/activate RIP2/RICK and this active NOD2:RIP2 complex then stimulates MAPK (JNK, ERK, p38), NF-kB, autophagy and antigen presentation. NOD2 has an ability to senses the presence of MDP (muramyl dipeptide), a breakdown product of peptidoglycan found in bacteria, and after recognition of intracellular bacteria, NOD2 pathway execute directing the secretion of antimicrobial peptides and cytokines/chemokines to induce innate immune clearance of the pathogen as well as to tailor the adaptive immune system to help fight the bacterial infection. Defects in NOD2 are the cause of Blau syndrome (BS), susceptibility to inflammatory bowel disease type 1 (IBD1) and sarcoidosis early-onset (EOS).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NOD2 Antibody (NB100-524)(25)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: FLOW, ICC/IF, IF/IHC, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using NB100-524 Applications Species
Li X, Liu S, Jin L et al. NOD2 inhibits the proliferation of esophageal adenocarcinoma cells through autophagy Journal of cancer research and clinical oncology 2022-10-31 [PMID: 36316517] (WB, Human) WB Human
Lipinski S, Petersen BS, Barann M et al. Missense variants in NOX1 and p22phox in a case of very-early-onset inflammatory bowel disease are functionally linked to NOD2 Cold Spring Harb Mol Case Stud. 2017-11-07 [PMID: 30709874] (WB, IP, Human) WB, IP Human
Tikka C, Manthari R, Niu R, et al. Dendritic cell CX3CR1 and macrophages F4/80 play a central role in between gut micro biome and inflammation in Arsenic induced mice bioRxiv 2021-01-26 (IF/IHC, Mouse) IF/IHC Mouse
Thakker P, Ariana A, Hajjar S et al. XIAP promotes the expansion and limits the contraction of CD8 T cell response through cell extrinsic and intrinsic mechanisms respectively PLoS pathogens 2023-06-22 [PMID: 37347786] (WB, Mouse) WB Mouse
Huaman M, Qualls J, Jose S et al. Mycobacterium bovis Bacille-Calmette-GuErin Infection Aggravates Atherosclerosis Frontiers in Immunology 2020-12-18 [PMID: 33391278] (FLOW, Mouse) FLOW Mouse
Mai E, Percopo CM, Limkar AR et al Respiratory Epithelial Cells Respond to Lactobacillus plantarum but Provide No Cross-Protection against Virus-Induced Inflammation Viruses 2020-12-30 [PMID: 33374950] (FLOW, Mouse)

Citation using the Azide Free version of this antibody.
FLOW Mouse
Zhang L, Zhang B, Wei M et Al. TRIM22 inhibits endometrial cancer progression through the NOD2/NF kappa B signaling pathway and confers a favorable prognosis Int J Oncol 2020-03-04 [PMID: 32319602] (ICC/IF, WB, IF/IHC, IHC-P, Human) ICC/IF, WB, IF/IHC, IHC-P Human
Tikka C, Manthari R, Ommati M, et al. Immune disruption occurs through altered gut microbiome and NOD2 in arsenic induced mice: Correlation with colon cancer markers Chemosphere 2020-01-01 [PMID: 31927375] (ICC/IF, IF/IHC, Mouse) ICC/IF, IF/IHC Mouse
Valdez-Miramontes CE, Trejo MartInez LA, Torres-JuArez F et al. Nicotine modulates molecules of the innate immune response in epithelial cells and macrophages during infection with M. tuberculosis Clin Exp Immunol. 2019-11-03 [PMID: 31631328]

Citation used the Alexa Fluor 647 format of this antibody.
Mey L, Jung M, Roos F et al. NOD1 and NOD2 of the innate immune system is differently expressed in human clear cell renal cell carcinoma, corresponding healthy renal tissue, its vasculature and primary isolated renal tubular epithelial cells J. Cancer Res. Clin. Oncol. 2019-03-22 [PMID: 30903318] (WB, FLOW, Human) WB, FLOW Human
Show All 25 Publications.

Review for NOD2 Antibody (NB100-524) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-524:
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Images Ratings Applications Species Date Details
Immunofluorescence NOD2 NB100-524
reviewed by:
Verified Customer
IF Mouse 10/03/2014


Sample TestedPFA fixed Mouse Sciatic Nerve Slice

Product General Protocols

View specific protocols for NOD2 Antibody (NB100-524): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NOD2 Antibody (NB100-524). (Showing 1 - 1 of 1 FAQs).

  1. Can you suggest the best NOD2 antibody for detection of endogenous NOD2 in human fibroblasts?
    • For detecting endogenous NOD2 in human samples, I would recommend NB100-524. This antibody has been validated for use in WB, IHC-Paraffin and IP.

Secondary Antibodies


Isotype Controls

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Recent Reviews


Verified Customer
Application: IF
Species: Mouse


Gene Symbol NOD2