NOD2 Antibody (2D9)

Images

 
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk ...read more
Immunocytochemistry/ Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Flow (Intracellular): NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on THP-1 cells with NOD2 (2D9) antibody NB100-524AF488 (blue) and a matched isotype control NBP2-27287AF488 (orange). Cells ...read more
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Western Blot Image of anti-NOD2 (2D9) Whole cell protein from THP-1 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat ...read more
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Detection of NOD2 in 20ug of NOD2 transfected 293T cell lysate using NB 100-524. ECL detection in 10 seconds.
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Detection of endogenous NOD2 in 50ug of HT29 cell lysate using NB 100-524. ECL detection in 15 minutes.
Immunocytochemistry/ Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - analysis of PFA fixed mouse sciatic nerve section using anti-NOD2 antibody. Image from verified customer review.
Flow (Intracellular): NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on THP-1 cells with NOD2 (2D9) antibody NB100-524AF488 (blue) and a matched isotype control NBP2-27287AF488 (orange). Cells ...read more
Flow (Intracellular): NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Raji cells with NOD2 (2D9) antibody NB100-524AF488 (blue) and a matched isotype control NBP2-27287AF488 (orange). Cells ...read more

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P, IP
Clone
2D9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

NOD2 Antibody (2D9) Summary

Immunogen
Recombinant human NOD2 protein corresponding to residues 28-301. [UniProt# Q9HC29]
Localization
Cytoplasmic
Predicted Species
Primate (92%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NOD2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Flow (Intracellular)
Application Notes
This NOD2 (2D9) antibody is useful for Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western blot. In WB, this antibody has been tested with NOD2 transfected 293T lysates and HT29 human colorectal adenocarcinoma lysates (endogenous detection has some background) where a band is seen at ~110 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in FLOW cytometry reported in scientific literature (PMID 28241127).
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB100-524 in the following applications:

Publications
Read Publications using
NB100-524 in the following applications:

  • 1 publication
  • 2 publications
  • IHC
    2 publications
  • 1 publication
  • IP
    1 publication
  • WB
    5 publications

Reactivity Notes

Human and mouse. Immunogen has 92% homology to primate.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for NOD2 Antibody (2D9)

  • BLAU
  • CARD15caspase recruitment domain protein 15
  • caspase recruitment domain family, member 15
  • Caspase recruitment domain-containing protein 15
  • CDACUG
  • CLR16.3
  • IBD1NLR family, CARD domain containing 2
  • Inflammatory bowel disease protein 1
  • NLRC2
  • NOD-like receptor C2
  • nucleotide-binding oligomerization domain 2
  • nucleotide-binding oligomerization domain containing 2
  • nucleotide-binding oligomerization domain, leucine rich repeat and CARD domaincontaining 2
  • nucleotide-binding oligomerization domain-containing protein 2
  • PSORAS1NOD2B

Background

The NOD2 (nucleotide-binding oligomerization domain containing 2) protein is expressed in the antigen-presenting cells (monocytes and macrophages) and in intestinal Paneth cells, keratinocytes, T cells, NK cells, and CD34+ stem cells. Alongwith Apaf-1 and CIITA, NOD2 belongs to NLR/NOD leucine-rich repeat containing protein family. NOD2 generally localizes to the cytoplasm from where it translocates to cell membranes, a mechanism suggested as important for its functionality. After interacting with CARD-CARD, NOD2 may bind/activate RIP2/RICK and this active NOD2:RIP2 complex then stimulates MAPK (JNK, ERK, p38), NF-kB, autophagy and antigen presentation. NOD2 has an ability to senses the presence of MDP (muramyl dipeptide), a breakdown product of peptidoglycan found in bacteria, and after recognition of intracellular bacteria, NOD2 pathway execute directing the secretion of antimicrobial peptides and cytokines/chemokines to induce innate immune clearance of the pathogen as well as to tailor the adaptive immune system to help fight the bacterial infection. Defects in NOD2 are the cause of Blau syndrome (BS), susceptibility to inflammatory bowel disease type 1 (IBD1) and sarcoidosis early-onset (EOS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-524
Species: Hu, Mu, Pm
Applications: WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P, IP

Publications for NOD2 Antibody (NB100-524)(13)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: Flow, ICC/IF, IHC, IHC-P, IP, WB.


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Flow
(1)
ICC/IF
(2)
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(2)
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(1)
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(1)
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(5)
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Human
(7)
Mouse
(2)
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB100-524 Applications Species
Subramanian K1, Bergman P, Henriques-Normark B. Vitamin D Promotes Pneumococcal Killing and Modulates Inflammatory Responses in Primary Human Neutrophils J Innate Immun. J Innate Immun. 2017 Feb 28 [PMID: 28241127] (Flow, Human)

Details:
This citation used the Alexa Fluor 488 version of this antibody.
Flow Human
Kapoor A, Forman M, Arav-Boger R. Activation of Nucleotide Oligomerization Domain 2 (NOD2) by Human Cytomegalovirus Initiates Innate Immune Responses and Restricts Virus Replication. PLoS ONE 3/27/2014 [PMID: 24671169] (WB, Human) WB Human
Lipinski S, Grabe N, Jacobs et al. RNAi screening identifies mediators of NOD2 signaling: Implications for spatial specificity of MDP recognition Proc Natl Acad Sci U S A 2012 Dec 3 [PMID: 23213202] (WB, IHC, ICC/IF, Human) WB, IHC, ICC/IF Human
Marischen L, Wesch D, Oberg HH et al. Functional expression of NOD2 in freshly isolated human peripheral blood ?d T-cells. Scand J Immunol. 2011 Mar 15. [PMID: 21410503]
Billmann-Born S, Till A, Arlt A et al. Genome-wide expression profiling identifies an impairment of negative feedback signals in the Crohn's disease-associated NOD2 variant L1007fsinsC J Immunol 2011 Apr [PMID: 21335489] (WB, Human) WB Human
von Kampen O, Lipinski S, Till A, Martin SJ, Nietfeld W, Lehrach H, Schreiber S, Rosenstiel P. Caspase recruitment domain-containing protein 8 (CARD8) negatively regulates NOD2-mediated signaling. J Biol Chem;285(26):19921-6. 2010 Jun 25. [PMID: 20385562] (ICC/IF, Human) ICC/IF Human
Cooney R, Baker J, Brain O, Danis B, Pichulik T, Allan P, Ferguson DJ, Campbell BJ, Jewell D, Simmons A. NOD2 stimulation induces autophagy in dendritic cells influencing bacterial handling and antigen presentation. Nat Med;16(1):90-7. 2010 Jan. [PMID: 19966812]
Lipinski S, Till A, Sina C et al. DUOX2-derived reactive oxygen species are effectors of NOD2-mediated antibacterial responses. J Cell Sci;122(19):3522-3530. 2009 [PMID: 19759286] (WB, IP, Human) WB, IP Human
Chauhan VS, Sterka DG Jr, Furr SR, Young AB, Marriott I. NOD2 plays an important role in the inflammatory responses of microglia and astrocytes to bacterial CNS pathogens. Glia;57(4):414-23. 2009 Mar. [PMID: 18803303] (WB, Mouse) WB Mouse
Kim TH, Payne U, Zhang X, Iwanaga Y, Davey MP, Rosenbaum JT, Inman RD. Altered host:pathogen interactions conferred by the Blau syndrome mutation of NOD2. Rheumatol Int;27(3):257-62. 2007 Jan. [PMID: 17096091]
Show All 13 Publications.

Review for NOD2 Antibody (NB100-524) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IF.

Reviews using NB100-524:
Filter by Applications
IF
(1)
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(1)
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Images Ratings Applications Species Date Details
Immunofluorescence NOD2 NB100-524
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reviewed by:
Alexander Chamessian
IF Mouse 10/03/2014
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Summary

ApplicationImmunofluorescence
Sample TestedPFA fixed Mouse Sciatic Nerve Slice
SpeciesMouse

Blocking

Blocking Details1% BSA w/ 0.01% Triton-X

Primary Anitbody

Dilution Ratio1:1000

Details

Fixation Details4% PFA with overnight post-fixation
Wash Description3x PBS wash for 5 minutes

Product General Protocols

View specific protocols for NOD2 Antibody (NB100-524): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NOD2 Antibody (NB100-524). (Showing 1 - 1 of 1 FAQs).

  1. Can you suggest the best NOD2 antibody for detection of endogenous NOD2 in human fibroblasts?
    • For detecting endogenous NOD2 in human samples, I would recommend NB100-524 (http://www.novusbio.com/NB100-524). This antibody has been validated for use in WB, IHC-Paraffin and IP.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-524AF405
Alexa Fluor 488 Labeled NB100-524AF488
Alexa Fluor 647 Labeled NB100-524AF647
Alexa Fluor 700 Labeled NB100-524AF700
Allophycocyanin Labeled NB100-524APC
Biotin Labeled NB100-524B
DyLight 350 Labeled NB100-524UV
DyLight 405 Labeled NB100-524V
DyLight 488 Labeled NB100-524G
DyLight 550 Labeled NB100-524R
DyLight 650 Labeled NB100-524C
DyLight 680 Labeled NB100-524FR
DyLight 755 Labeled NB100-524IR
FITC Labeled NB100-524F
HRP Labeled NB100-524H
PE Labeled NB100-524PE
PerCP Labeled NB100-524PCP

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Bioinformatics Tool for NOD2 Antibody (NB100-524)

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Blogs on NOD2.

Transferrin and the blood brain barrier
Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

CD3 (OKT3) as a marker of immune response efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.

NOD2 - inflammatory signaling and NFkB activation
Nucleotide-binding oligomerization domain-containing protein 2 (NOD2) is an intracellular pattern recognition receptor (PRR) that plays an important role in recognizing bacterial pathogens and initiating an immune response. As a PRR, NOD2 recogniz...  Read full blog post.

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Alexander Chamessian
10/03/2014
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol NOD2