NOD2 Antibody (2D9)


Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs more
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk more
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
Flow (Intracellular): NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on THP-1 cells with NOD2 (2D9) antibody NB100-524AF488 (blue) and a matched isotype control NBP2-27287AF488 (orange). Cells more
Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and more
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Whole cell protein from THP-1 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed more
Immunohistochemistry: NOD2 Antibody (2D9) [NB100-524] - Analysis of PFA fixed mouse sciatic nerve section using anti-NOD2 antibody. Image from verified customer review.
Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA more

Product Details

Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP
1.0 mg/ml

Order Details

NOD2 Antibody (2D9) Summary

Recombinant human NOD2 protein corresponding to residues 28-301. [UniProt# Q9HC29]
Predicted Species
Primate (92%). Backed by our 100% Guarantee.
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Flow (Intracellular)
Application Notes
This NOD2 (2D9) antibody is useful for Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western blot. In WB, this antibody has been tested with NOD2 transfected 293T lysates and HT29 human colorectal adenocarcinoma lysates (endogenous detection has some background) where a band is seen at ~110 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in FLOW cytometry reported in scientific literature (PMID: 28241127).
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
NB100-524 in the following applications:

Read Publications using
NB100-524 in the following applications:

  • 4 publications
  • 4 publications
  • IHC
    4 publications
  • 2 publications
  • IP
    3 publications
  • WB
    9 publications

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for NOD2 Antibody (2D9)

  • BLAU
  • CARD15caspase recruitment domain protein 15
  • caspase recruitment domain family, member 15
  • Caspase recruitment domain-containing protein 15
  • CLR16.3
  • IBD1NLR family, CARD domain containing 2
  • Inflammatory bowel disease protein 1
  • NLRC2
  • NOD-like receptor C2
  • nucleotide-binding oligomerization domain 2
  • nucleotide-binding oligomerization domain containing 2
  • nucleotide-binding oligomerization domain, leucine rich repeat and CARD domaincontaining 2
  • nucleotide-binding oligomerization domain-containing protein 2


The NOD2 (nucleotide-binding oligomerization domain containing 2) protein is expressed in the antigen-presenting cells (monocytes and macrophages) and in intestinal Paneth cells, keratinocytes, T cells, NK cells, and CD34+ stem cells. Alongwith Apaf-1 and CIITA, NOD2 belongs to NLR/NOD leucine-rich repeat containing protein family. NOD2 generally localizes to the cytoplasm from where it translocates to cell membranes, a mechanism suggested as important for its functionality. After interacting with CARD-CARD, NOD2 may bind/activate RIP2/RICK and this active NOD2:RIP2 complex then stimulates MAPK (JNK, ERK, p38), NF-kB, autophagy and antigen presentation. NOD2 has an ability to senses the presence of MDP (muramyl dipeptide), a breakdown product of peptidoglycan found in bacteria, and after recognition of intracellular bacteria, NOD2 pathway execute directing the secretion of antimicrobial peptides and cytokines/chemokines to induce innate immune clearance of the pathogen as well as to tailor the adaptive immune system to help fight the bacterial infection. Defects in NOD2 are the cause of Blau syndrome (BS), susceptibility to inflammatory bowel disease type 1 (IBD1) and sarcoidosis early-onset (EOS).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NOD2 Antibody (NB100-524)(22)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: Flow, ICC/IF, IHC, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NB100-524 Applications Species
Huaman M, Qualls J, Jose S et al. Mycobacterium bovis Bacille-Calmette-GuErin Infection Aggravates Atherosclerosis Frontiers in Immunology Dec 18 2020 [PMID: 33391278] (Flow, Mouse) Flow Mouse
Mai E, Percopo CM, Limkar AR et al. Respiratory Epithelial Cells Respond to Lactobacillus plantarum but Provide No Cross-Protection against Virus-Induced Inflammation Viruses Dec 22 2020 [PMID: 33374950] (Flow, Mouse) Flow Mouse
Zhang L, Zhang B, Wei M et Al. TRIM22 inhibits endometrial cancer progression through the NOD2/NF kappa B signaling pathway and confers a favorable prognosis Int J Oncol Mar 4 2020 [PMID: 32319602] (IHC-P, IHC, ICC/IF, WB, Human) IHC-P, IHC, ICC/IF, WB Human
Tikka C, Manthari R, Ommati M, et al. Immune disruption occurs through altered gut microbiome and NOD2 in arsenic induced mice: Correlation with colon cancer markers Chemosphere Jan 1 2020 [PMID: 31927375] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Valdez-Miramontes CE, Trejo MartInez LA, Torres-JuArez F et al. Nicotine modulates molecules of the innate immune response in epithelial cells and macrophages during infection with M. tuberculosis Clin Exp Immunol. 2019 Nov 3 [PMID: 31631328]

Citation used the Alexa Fluor 647 format of this antibody.
Mey L, Jung M, Roos F et al. NOD1 and NOD2 of the innate immune system is differently expressed in human clear cell renal cell carcinoma, corresponding healthy renal tissue, its vasculature and primary isolated renal tubular epithelial cells J. Cancer Res. Clin. Oncol. Mar 22 2019 [PMID: 30903318] (WB, Flow, Human) WB, Flow Human
Lipinski S, Petersen BS, Barann M et al. Missense variants in NOX1 and p22phox in a case of very-early-onset inflammatory bowel disease are functionally linked to NOD2 Cold Spring Harb Mol Case Stud. 2017 Nov 07 [PMID: 30709874] (WB, IP, Human) WB, IP Human
Singh K, Han K, Tilve S et al. Parkin targets NOD2 to regulate astrocyte endoplasmic reticulum stress and inflammation. Glia. Nov 1 2018 [PMID: 30378174] (IP, Mouse) IP Mouse
Li J, Li B, Cheng Y et al. The synergistic effect of NOD2 and TLR4 on the activation of autophagy in human submandibular gland inflammation. J. Oral Pathol. Med. Oct 26 2018 [PMID: 30367515] (WB, Human) WB Human
Subramanian K1, Bergman P, Henriques-Normark B. Vitamin D Promotes Pneumococcal Killing and Modulates Inflammatory Responses in Primary Human Neutrophils J Innate Immun. J Innate Immun. 2017 Feb 28 [PMID: 28241127] (Flow, Human)

This citation used the Alexa Fluor 488 version of this antibody.
Flow Human
Show All 22 Publications.

Review for NOD2 Antibody (NB100-524) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-524:
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Images Ratings Applications Species Date Details
Immunofluorescence NOD2 NB100-524
reviewed by:
Alexander Chamessian
IF Mouse 10/03/2014


Sample TestedPFA fixed Mouse Sciatic Nerve Slice


Blocking Details1% BSA w/ 0.01% Triton-X

Primary Anitbody

Dilution Ratio1:1000


Fixation Details4% PFA with overnight post-fixation
Wash Description3x PBS wash for 5 minutes

Product General Protocols

View specific protocols for NOD2 Antibody (NB100-524): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NOD2 Antibody (NB100-524). (Showing 1 - 1 of 1 FAQs).

  1. Can you suggest the best NOD2 antibody for detection of endogenous NOD2 in human fibroblasts?
    • For detecting endogenous NOD2 in human samples, I would recommend NB100-524 ( This antibody has been validated for use in WB, IHC-Paraffin and IP.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-524AF350
Alexa Fluor 405 NB100-524AF405
Alexa Fluor 488 NB100-524AF488
Alexa Fluor 594 NB100-524AF594
Alexa Fluor 647 NB100-524AF647
Alexa Fluor 700 NB100-524AF700
Alexa Fluor 750 NB100-524AF750
Allophycocyanin NB100-524APC
Biotin NB100-524B
DyLight 350 NB100-524UV
DyLight 405 NB100-524V
DyLight 488 NB100-524G
DyLight 550 NB100-524R
DyLight 594 NB100-524DL594
DyLight 650 NB100-524C
DyLight 680 NB100-524FR
DyLight 755 NB100-524IR
FITC NB100-524F
HRP NB100-524H
Janelia Fluor 549 NB100-524JF549
Janelia Fluor 646 NB100-524JF646
PE NB100-524PE

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Blogs on NOD2.

Pattern recognition receptors (PRR) expression and function in endothelial cells
By Victoria OsinskiWhat are Pattern recognition receptors?Pattern recognition receptors (PRRs) recognize and respond to Pattern- and Damage-associated molecular patterns (PAMP and DAMPs) in a number of different c...  Read full blog post.

Transferrin and the blood brain barrier
Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

CD3 (OKT3) as a marker of immune response efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.

NOD2 - inflammatory signaling and NFkB activation
Nucleotide-binding oligomerization domain-containing protein 2 (NOD2) is an intracellular pattern recognition receptor (PRR) that plays an important role in recognizing bacterial pathogens and initiating an immune response. As a PRR, NOD2 recogniz...  Read full blog post.

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Recent Reviews


Alexander Chamessian
Application: IF
Species: Mouse


Gene Symbol NOD2