CXCL8/IL-8 Antibody (6217) [Unconjugated]


IL-8/CXCL8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) at 10 µg/mL for 3 hours at room temperature. Cells more
Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the more

Product Details

Reactivity HuSpecies Glossary
Applications WB, B/N, CyTOF-ready, ELISA(Cap), Flow, ICC/IF

CXCL8/IL-8 Antibody (6217) [Unconjugated] Summary

E. coli-derived recombinant human IL-8/CXCL8
Accession # P10145
Detects human IL-8/CXCL8 in ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant porcine IL-8/CXCL8 and no cross-reactivity with recombinant rat CXCL3/CINC-2 alpha is observed.
Details of Functionality
ActivityAssaywCitation not found None None None None None
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1 ug/mL
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.08-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Human CXCL8/IL-8 Biotinylated Antibody (Catalog number BAF208)
Standard: Recombinant Human CXCL8/IL-8 (Catalog number 208-IL)
Read Publications using
MAB208 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL8/IL-8 Antibody (6217) [Unconjugated]

  • 3-10C
  • AMCF-I
  • C-X-C motif chemokine 8
  • CXCL8
  • Emoctakin
  • GCP1
  • GCP-1TSG-1
  • IL8
  • IL-8
  • interleukin 8
  • K60
  • LAI
  • LECT
  • member 8
  • NAP1
  • NAP-1NAP1
  • NCF
  • Neutrophil-activating protein 1
  • Protein 3-10C
  • T cell chemotactic factor
  • T-cell chemotactic factor
  • TCF
  • TSG1


CXCL8, also known as IL-8, is a CXC chemokine that is a potent chemoattractant for neutrophils and has a wide range of other pro-inflammatory effects.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCL8/IL-8 Antibody (MAB208)(79)

We have publications tested in 6 confirmed species: Human, Bacteria - Porphyromonas gingivalis, Bovine, Complex Species Category, E. coli, Guinea Pig.

We have publications tested in 18 applications: Affinity Chromatography, Antibody Array Development, Array Development, Bioassay, ELISA Capture, ELISA Development, ELISA detection, Electrochemiluminescence, Functional Assay, ICC, IHC Fresh, IHC Frozen, IHC Paraffin-embedded, IP, Luminex Development, Neut, WB, inhibition.

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Affinity Chromatography
Antibody Array Development
Array Development
ELISA Capture
ELISA Development
ELISA detection
Functional Assay
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IHC Paraffin-embedded
Luminex Development
All Applications
Filter By Species
Bacteria - Porphyromonas gingivalis
Complex Species Category
E. coli
Guinea Pig
All Species
Showing Publications 1 - 10 of 79. Show All 79 Publications.
Publications using MAB208 Applications Species
A Micera, A Di Zazzo, G Esposito, R Longo, W Foulsham, R Sacco, R Sgrulletta, S Bonini Age-Related Changes to Human Tear Composition Invest. Ophthalmol. Vis. Sci., 2018;59(5):2024-2031. 2018 [PMID: 29677365] (WB,, Human) WB Human
Q Liu, A Li, S Yu, S Qin, N Han, RG Pestell, X Han, K Wu DACH1 antagonizes CXCL8 to repress tumorigenesis of lung adenocarcinoma and improve prognosis J Hematol Oncol, 2018;11(1):53. 2018 [PMID: 29636079] (Bioassay, Human) Bioassay Human
F Melo-Gonza, TM Fenton, C Forss, C Smedley, A Goenka, AS MacDonald, DJ Thornton, MA Travis Intestinal mucin activates human dendritic cells and IL-8 production in a glycan-specific manner J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29581231] (Neut,, Human) Neut Human
S di Martino, CA Amoreo, B Nuvoli, R Galati, S Strano, F Facciolo, G Alessandri, HI Pass, G Ciliberto, G Blandino, R De Maria, M Cioce HSP90 inhibition alters the chemotherapy-driven rearrangement of the oncogenic secretome Oncogene, 2018;0(0):. 2018 [PMID: 29311642] (inhibition, Human) inhibition Human
J Ikari, AJ Nelson, J Obaid, A Giron-Mart, K Ikari, F Makino, S Iwasawa, Y Gunji, M Farid, X Wang, H Basma, D Demeo, C Feghali-Bo, O Holz, K Rabe, X Liu, SI Rennard Reduced microRNA-503 expression augments lung fibroblast VEGF production in chronic obstructive pulmonary disease PLoS ONE, 2017;12(9):e0184039. 2017 [PMID: 28880936] (ELISA Capture,, Human) ELISA Capture Human
MM White, P Geraghty, E Hayes, S Cox, W Leitch, B Alfawaz, GM Lavelle, OJ McElvaney, R Flannery, J Keenan, P Meleady, M Henry, M Clynes, C Gunaratnam, NG McElvaney, EP Reeves Neutrophil Membrane Cholesterol Content is a Key Factor in Cystic Fibrosis Lung Disease EBioMedicine, 2017;23(0):173-184. 2017 [PMID: 28835336] (ELISA Capture,, Human) ELISA Capture Human
B Tian, A Al-Moujahe, P Bouzika, Y Hu, S Notomi, P Tsoka, JW Miller, H Lin, DG Vavvas Atorvastatin Promotes Phagocytosis and Attenuates Pro-Inflammatory Response in Human Retinal Pigment Epithelial Cells Sci Rep, 2017;7(1):2329. 2017 [PMID: 28539592] (WB,, Human) WB Human
Y Wang, J Liu, Q Jiang, J Deng, F Xu, X Chen, F Cheng, Y Zhang, Y Yao, Z Xia, X Xu, X Su, M Huang, L Dai, Y Yang, S Zhang, D Yu, RC Zhao, Y Wei, H Deng Human adipose-derived mesenchymal stem cell-secreted CXCL1 and CXCL8 facilitate breast tumor growth by promoting angiogenesis Stem Cells, 2017;0(0):. 2017 [PMID: 28514506] (Neut,, Human) Neut Human
J Liu, G Song, X Lin, X Pang, T Meng Upregulated unique long 16 binding protein 1 detected in preeclamptic placenta affects human extravillous trophoblast cell line (HTR-8/SVneo) invasion by modulating the function of uterine natural killer cells Exp Ther Med, 2017;13(4):1447-1455. 2017 [PMID: 28413491] (Functional Assay,, Human) Functional Assay Human
HB Song, HO Jun, JH Kim, YH Lee, MH Choi, JH Kim Disruption of outer blood-retinal barrier by Toxoplasma gondii-infected monocytes is mediated by paracrinely activated FAK signaling PLoS ONE, 2017;12(4):e0175159. 2017 [PMID: 28406972] (Neut,, Human) Neut Human
Show All 79 Publications.

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Gene Symbol IL8