CXCL8/IL-8 Antibody (6217) [Unconjugated]

Images

 
Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more
IL-8/CXCL8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) at 10 µg/mL for 3 hours at room temperature. Cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, B/N, CyTOF-ready, ELISA(Cap), Flow, ICC/IF
Clone
6217
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CXCL8/IL-8 Antibody (6217) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IL-8/CXCL8
Ser28-Ser99
Accession # P10145
Specificity
Detects human IL-8/CXCL8 in ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant porcine IL-8/CXCL8 and no cross-reactivity with recombinant rat CXCL3/CINC-2 alpha is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL8
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization 0.08-0.4 ug/mL
  • Simple Western 50 ug/mL
  • Western Blot 3 ug/mL
Application Notes
ELISA Detection: Human CXCL8/IL-8 Biotinylated Antibody (Catalog number BAF208)
Standard: Recombinant Human CXCL8/IL-8 (Catalog number 208-IL)
Reviewed Applications
Read 1 Review rated 4
using
MAB208 in the following applications:

Publications
Read Publications using
MAB208 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL8/IL-8 Antibody (6217) [Unconjugated]

  • 3-10C
  • AMCF-I
  • C-X-C motif chemokine 8
  • CXCL8
  • CXCL8SCYB8
  • Emoctakin
  • GCP1
  • GCP-1TSG-1
  • IL8
  • IL-8
  • interleukin 8
  • K60
  • LAI
  • LECT
  • LUCT
  • LYNAP
  • MDNCF
  • MDNCFb-ENAP
  • member 8
  • MONAP
  • MONAPGCP1
  • NAF
  • NAP1
  • NAP-1NAP1
  • NCF
  • Neutrophil-activating protein 1
  • Protein 3-10C
  • T cell chemotactic factor
  • T-cell chemotactic factor
  • TCF
  • TSG1

Background

CXCL8, also known as IL-8, is a CXC chemokine that is a potent chemoattractant for neutrophils and has a wide range of other pro-inflammatory effects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCL8/IL-8 Antibody (MAB208)(100)

We have publications tested in 7 confirmed species: Human, Rat, Bacteria - E. Coli, Bacteria - Porphyromonas gingivalis, Bovine, Complex Species Category, Guinea Pig.

We have publications tested in 21 applications: Affinity Chromatography, Antibody Array Development, Array Development, Binding Assay, Bioassay, Blocking, Co-Immunoprecipitation, ELISA Development, ELISA Development (Capture), ELISA Development (Detection), Electrochemiluminescent Assay, Functional Assay, ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Inhibition, Luminex Development, Neutralization, Western Blot.


Filter By Application
Affinity Chromatography
(1)
Antibody Array Development
(1)
Array Development
(1)
Binding Assay
(1)
Bioassay
(7)
Blocking
(1)
Co-Immunoprecipitation
(1)
ELISA Development
(21)
ELISA Development (Capture)
(4)
ELISA Development (Detection)
(1)
Electrochemiluminescent Assay
(2)
Functional Assay
(1)
ICC
(6)
IHC
(1)
IHC-Fr
(2)
IHC-P
(3)
Immunoprecipitation
(1)
Inhibition
(1)
Luminex Development
(1)
Neutralization
(33)
Western Blot
(10)
All Applications
Filter By Species
Human
(94)
Rat
(1)
Bacteria - E. Coli
(1)
Bacteria - Porphyromonas gingivalis
(1)
Bovine
(1)
Complex Species Category
(1)
Guinea Pig
(1)
All Species
Showing Publications 1 - 10 of 100. Show All 100 Publications.
Publications using MAB208 Applications Species
F Yang, XQ Liu, JZ He, SP Xian, PF Yang, ZY Mai, M Li, Y Liu, XD Zhang Occludin facilitates tumour angiogenesis in bladder cancer by regulating IL8/STAT3 through STAT4 Journal of Cellular and Molecular Medicine, 2022;0(0):. 2022 [PMID: 35224833] (Bioassay, Human) Bioassay Human
RM Urbantat, C Jelgersma, S Brandenbur, M Nieminen-K, I Kremenetsk, J Zollfrank, S Mueller, K Rubarth, A Koch, P Vajkoczy, G Acker Tumor-Associated Microglia/Macrophages as a Predictor for Survival in Glioblastoma and Temozolomide-Induced Changes in CXCR2 Signaling with New Resistance Overcoming Strategy by Combination Therapy International Journal of Molecular Sciences, 2021;22(20):. 2021 [PMID: 34681839] (IHC, Human) IHC Human
Q Hou, S Chen, Q An, B Li, Y Fu, Y Luo Extracellular Hsp90alpha Promotes Tumor Lymphangiogenesis and Lymph Node Metastasis in Breast Cancer International Journal of Molecular Sciences, 2021;22(14):. 2021 [PMID: 34299365] (Co-Immunoprecipitation, Human) Co-Immunoprecipitation Human
S Gonçalves, K Yin, Y Ito, A Chan, I Olan, S Gough, L Cassidy, E Serrao, S Smith, A Young, M Narita, M Hoare COX2 regulates senescence secretome composition and senescence surveillance through PGE2 Cell Reports, 2021;34(11):108860. 2021 [PMID: 33730589] (Western Blot, Human) Western Blot Human
H Israelov, O Ravid, D Atrakchi, D Rand, S Elhaik, Y Bresler, R Twitto-Gre, L Omesi, S Liraz-Zalt, F Gosselet, M Schnaider, I Cooper Caspase-1 has a critical role in blood-brain barrier injury and its inhibition contributes to multifaceted repair J Neuroinflammation, 2020;17(1):267. 2020 [PMID: 32907600] (Neutralization, Human) Neutralization Human
H Kikuchi, N Maishi, DA Annan, M Towfik Ala, R Ibrahim Ha, M Sato, M Morimoto, R Takeda, K Ishizuka, R Matsumoto, T Akino, K Tsuchiya, T Abe, T Osawa, N Miyajima, S Maruyama, T Harabayash, M Azuma, K Yamashiro, K Ameda, A Kashiwagi, Y Matsuno, Y Hida, N Shinohara, K Hida Chemotherapy-induced IL-8 upregulates MDR1/ABCB1 in tumor blood vessels and results in unfavorable outcome Cancer Res., 2020;0(0):. 2020 [PMID: 32536602] (Neutralization, Human) Neutralization Human
Q Ji, L Zhou, H Sui, L Yang, X Wu, Q Song, R Jia, R Li, J Sun, Z Wang, N Liu, Y Feng, X Sun, G Cai, Y Feng, J Cai, Y Cao, G Cai, Y Wang, Q Li Primary tumors release ITGBL1-rich extracellular vesicles to promote distal metastatic tumor growth through fibroblast-niche formation Nat Commun, 2020;11(1):1211. 2020 [PMID: 32139701] (Neutralization, Human) Neutralization Human
LM Ashander, S Lie, Y Ma, E Rochet, JM Washington, JM Furtado, B Appukuttan, JR Smith Neutrophil Activities in Human Ocular Toxoplasmosis: An In Vitro Study With Human Cells Invest. Ophthalmol. Vis. Sci., 2019;60(14):4652-4660. 2019 [PMID: 31743940] (Neutralization, Human) Neutralization Human
R Eyre, DG Alférez, A Santiago-G, K Spence, JC McConnell, C Hart, BM Simões, D Lefley, C Tulotta, J Storer, A Gurney, N Clarke, M Brown, SJ Howell, AH Sims, G Farnie, PD Ottewell, RB Clarke Microenvironmental IL1beta promotes breast cancer metastatic colonisation in the bone via activation of Wnt signalling Nat Commun, 2019;10(1):5016. 2019 [PMID: 31676788] (Neutralization, Human) Neutralization Human
A Schulz, L Jiang, L de Vor, M Ehrström, F Wermeling, L Eidsmo, K Melican Neutrophil Recruitment to Noninvasive MRSA at the Stratum Corneum of Human Skin Mediates Transient Colonization Cell Rep, 2019;29(5):1074-1081.e5. 2019 [PMID: 31665625] (Blocking, Human) Blocking Human
Show All 100 Publications.

Review for CXCL8/IL-8 Antibody (MAB208) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB208:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin CXCL8/IL-8 MAB208
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4
reviewed by:
Verified Customer
IHC-P Human 08/30/2018
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman lymph node (melanoma)
SpeciesHuman
LotASJ3517041

Comments

CommentsHuman FFPE metastatic melanoma lymph node sections were stained with anti-human IL-8 monoclonal antibody &#40;MAB208&#41; at 5 µg/ml followed by secondary goat anti-mouse biotinylated antibody and Streptavidin AP conjugate. Detection via an Alkaline Phosphatase/Red staining System.
Blocking: 5 % goat Serum in TBS-T
Antigen Retrieval: Citrat buffer
Primary antibody incubation: over night at 4 °C

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Verified Customer
08/30/2018
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol IL8
Uniprot