CXCL8/IL-8 Antibody (6217) [Unconjugated]

Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA and 10 ug/mL LPS for 24 hours and 3 hours. PVDF membrane was probed with 3 µg/mL of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for IL-8/CXCL8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

IL-8/CXCL8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Simple Western lane view shows human PBMC conditioned media untreated (-) or treated (+) with 1 μg/mL LPS for 24 hrs, loaded at 0.2 mg/mL. A specific band was detected for IL-8/CXCL8 at approximately 8 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) . This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208). The ND50 is typically0.08-0.4 µg/mL.

• Reactivity: Hu
• Applications: WB, Simple Western, B/N, CyTOF-ready, ELISA(Cap), Flow, ICC/IF
• Clone: 6217
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CXCL8/IL-8 Antibody (6217) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IL-8/CXCL8
  Ser28-Ser99
  Accession # P10145
• Specificity: Detects human IL-8/CXCL8 in ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant porcine IL-8/CXCL8 and no cross-reactivity with recombinant rat CXCL3/CINC-2 alpha is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL8
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization 0.08-0.4 ug/mL
  • Simple Western 50 ug/mL
  • Western Blot 3 ug/mL
• Application Notes: ELISA Detection: Human CXCL8/IL-8 Biotinylated Antibody (Catalog number BAF208)
  Standard: Recombinant Human CXCL8/IL-8 (Catalog number 208-IL)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL8/IL-8 Antibody (6217) [Unconjugated]

• 3-10C
• AMCF-I
• C-X-C motif chemokine 8
• CXCL8
• CXCL8SCYB8
• Emoctakin
• GCP1
• GCP-1TSG-1
• IL8
• IL-8
• interleukin 8
• K60
• LAI
• LECT
• LUCT
• LYNAP
• MDNCF
• MDNCFb-ENAP
• member 8
• MONAP
• MONAPGCP1
• NAF
• NAP1
• NAP-1NAP1
• NCF
• Neutrophil-activating protein 1
• Protein 3-10C
• T cell chemotactic factor
• T-cell chemotactic factor
• TCF
• TSG1

Background

CXCL8, also known as IL-8, is a CXC chemokine that is a potent chemoattractant for neutrophils and has a wide range of other pro-inflammatory effects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL8/IL-8 Antibody (MAB208)(110)

Publications using MAB208

• S Lee, YJ Seo, JY Choi, X Che, HJ Kim, SY Eum, MS Hong, SK Lee, DC Cho Effect of teriparatide on drug treatment of tuberculous spondylitis: an experimental study Scientific Reports, 2022;12(1):21667. 2022-01-01 [PMID: 36522387] (ELISA Capture, Human)
• W Zeng, L Xiong, W Wu, S Li, J Liu, L Yang, L Lao, P Huang, M Zhang, H Chen, N Miao, Z Lin, Z Liu, X Yang, J Wang, P Wang, E Song, Y Yao, Y Nie, J Chen, D Huang CCL18 signaling from tumor-associated macrophages activates fibroblasts to adopt a chemoresistance-inducing phenotype Oncogene, 2022;0(0):. 2022-01-01 [PMID: 36418470] (Flow Cytometry, Human)
• S Sun, L Jin, Y Zheng, J Zhu Modeling human HSV infection via a vascularized immune-competent skin-on-chip platform Nature Communications, 2022;13(1):5481. 2022-01-01 [PMID: 36123328] (Neutralization, Human)
• MF Santolla, M Talia, F Cirillo, D Scordamagl, S De Rosis, A Spinelli, AM Miglietta, B Nardo, G Filippelli, EM De Frances, A Belfiore, R Lappano, M Maggiolini The AGEs/RAGE Transduction Signaling Prompts IL-8/CXCR1/2-Mediated Interaction between Cancer-Associated Fibroblasts (CAFs) and Breast Cancer Cells Cells, 2022;11(15):. 2022-01-01 [PMID: 35954247] (Bioassay, Human)
• R Islam, J Mishra, NS Polavaram, S Bhattachar, Z Hong, S Bodas, S Sharma, A Bouska, T Gilbreath, AM Said, LM Smith, BA Teply, MH Muders, SK Batra, K Datta, S Dutta Neuropilin-2 axis in regulating secretory phenotype of neuroendocrine-like prostate cancer cells and its implication in therapy resistance Oncogene, 2022;40(3):111097. 2022-01-01 [PMID: 35858551] (Western Blot, Human)
• T Walle, JA Kraske, B Liao, B Lenoir, C Timke, E von Bohlen, F Tran, P Griebel, D Albrecht, A Ahmed, M Suarez-Car, A Jiménez-Sá, T Beikert, A Tietz-Dahl, AN Menevse, G Schmidt, M Brom, JHW Pahl, W Antonopoul, M Miller, RL Perez, F Bestvater, NA Giese, P Beckhove, P Rosenstiel, D Jäger, O Strobel, D Pe'er, N Halama, J Debus, A Cerwenka, PE Huber Radiotherapy orchestrates natural killer cell dependent antitumor immune responses through CXCL8 Science Advances, 2022;8(12):eabh4050. 2022-01-01 [PMID: 35319989] (ICC, Human)
• E Lieschke, Z Wang, C Chang, CE Weeden, GL Kelly, A Strasser Flow cytometric single cell-based assay to simultaneously detect cell death, cell cycling, DNA content and cell senescence Cell Death and Differentiation, 2022;0(0):. 2022-01-01 [PMID: 35264779] (ICC, Human)
• F Yang, XQ Liu, JZ He, SP Xian, PF Yang, ZY Mai, M Li, Y Liu, XD Zhang Occludin facilitates tumour angiogenesis in bladder cancer by regulating IL8/STAT3 through STAT4 Journal of Cellular and Molecular Medicine, 2022;0(0):. 2022-01-01 [PMID: 35224833] (Bioassay, Human)
• RM Urbantat, C Jelgersma, S Brandenbur, M Nieminen-K, I Kremenetsk, J Zollfrank, S Mueller, K Rubarth, A Koch, P Vajkoczy, G Acker Tumor-Associated Microglia/Macrophages as a Predictor for Survival in Glioblastoma and Temozolomide-Induced Changes in CXCR2 Signaling with New Resistance Overcoming Strategy by Combination Therapy International Journal of Molecular Sciences, 2021;22(20):. 2021-01-01 [PMID: 34681839] (IHC, Human)
• Q Hou, S Chen, Q An, B Li, Y Fu, Y Luo Extracellular Hsp90alpha Promotes Tumor Lymphangiogenesis and Lymph Node Metastasis in Breast Cancer International Journal of Molecular Sciences, 2021;22(14):. 2021-01-01 [PMID: 34299365] (Co-Immunoprecipitation, Human)
• S Gonçalves, K Yin, Y Ito, A Chan, I Olan, S Gough, L Cassidy, E Serrao, S Smith, A Young, M Narita, M Hoare COX2 regulates senescence secretome composition and senescence surveillance through PGE2 Cell Reports, 2021;34(11):108860. 2021-01-01 [PMID: 33730589] (Western Blot, Human)
• H Israelov, O Ravid, D Atrakchi, D Rand, S Elhaik, Y Bresler, R Twitto-Gre, L Omesi, S Liraz-Zalt, F Gosselet, M Schnaider, I Cooper Caspase-1 has a critical role in blood-brain barrier injury and its inhibition contributes to multifaceted repair J Neuroinflammation, 2020;17(1):267. 2020-01-01 [PMID: 32907600] (Neutralization, Human)
• H Kikuchi, N Maishi, DA Annan, M Towfik Ala, R Ibrahim Ha, M Sato, M Morimoto, R Takeda, K Ishizuka, R Matsumoto, T Akino, K Tsuchiya, T Abe, T Osawa, N Miyajima, S Maruyama, T Harabayash, M Azuma, K Yamashiro, K Ameda, A Kashiwagi, Y Matsuno, Y Hida, N Shinohara, K Hida Chemotherapy-induced IL-8 upregulates MDR1/ABCB1 in tumor blood vessels and results in unfavorable outcome Cancer Res., 2020;0(0):. 2020-01-01 [PMID: 32536602] (Neutralization, Human)
• Q Ji, L Zhou, H Sui, L Yang, X Wu, Q Song, R Jia, R Li, J Sun, Z Wang, N Liu, Y Feng, X Sun, G Cai, Y Feng, J Cai, Y Cao, G Cai, Y Wang, Q Li Primary tumors release ITGBL1-rich extracellular vesicles to promote distal metastatic tumor growth through fibroblast-niche formation Nat Commun, 2020;11(1):1211. 2020-01-01 [PMID: 32139701] (Neutralization, Human)
• LM Ashander, S Lie, Y Ma, E Rochet, JM Washington, JM Furtado, B Appukuttan, JR Smith Neutrophil Activities in Human Ocular Toxoplasmosis: An In Vitro Study With Human Cells Invest. Ophthalmol. Vis. Sci., 2019;60(14):4652-4660. 2019-01-01 [PMID: 31743940] (Neutralization, Human)
• R Eyre, DG Alférez, A Santiago-G, K Spence, JC McConnell, C Hart, BM Simões, D Lefley, C Tulotta, J Storer, A Gurney, N Clarke, M Brown, SJ Howell, AH Sims, G Farnie, PD Ottewell, RB Clarke Microenvironmental IL1beta promotes breast cancer metastatic colonisation in the bone via activation of Wnt signalling Nat Commun, 2019;10(1):5016. 2019-01-01 [PMID: 31676788] (Neutralization, Human)
• A Schulz, L Jiang, L de Vor, M Ehrström, F Wermeling, L Eidsmo, K Melican Neutrophil Recruitment to Noninvasive MRSA at the Stratum Corneum of Human Skin Mediates Transient Colonization Cell Rep, 2019;29(5):1074-1081.e5. 2019-01-01 [PMID: 31665625] (Blocking, Human)
• R Lamichhane, M Schneider, SM de la Harp, TWR Harrop, RF Hannaway, PK Dearden, JR Kirman, JDA Tyndall, AJ Vernall, JE Ussher TCR- or Cytokine-Activated CD8+ Mucosal-Associated Invariant T Cells Are Rapid Polyfunctional Effectors That Can Coordinate Immune Responses Cell Rep, 2019;28(12):3061-3076.e5. 2019-01-01 [PMID: 31533031] (Bioassay, Human)
• M Borghesan, J Fafián-Lab, O Eleftheria, P Carpintero, M Paez-Ribes, G Vizcay-Bar, A Swisa, D Kolodkin-G, P Ximénez-Em, R Lowe, B Martín-Mar, H Peinado, J Muñoz, RA Fleck, Y Dor, I Ben-Porath, A Vossenkamp, D Muñoz-Espi, A O'Loghlen Small Extracellular Vesicles Are Key Regulators of Non-cell Autonomous Intercellular Communication in Senescence via the Interferon Protein IFITM3 Cell Rep, 2019;27(13):3956-3971.e6. 2019-01-01 [PMID: 31242426] (Western Blot, Human)
• DP Muñoz, SM Yannone, A Daemen, Y Sun, F Vakar-Lope, M Kawahara, AM Freund, F Rodier, JD Wu, PY Desprez, DH Raulet, PS Nelson, LJ van 't Vee, J Campisi, JP Coppé Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging JCI Insight, 2019;5(0):. 2019-01-01 [PMID: 31184599] (IHC, Human)
• L Miarka, C Hauser, O Helm, D Holdhof, S Beckinger, JH Egberts, JP Gundlach, L Lenk, S Rahn, W Mikulits, A Trauzold, S Sebens The Hepatic Microenvironment and TRAIL-R2 Impact Outgrowth of Liver Metastases in Pancreatic Cancer after Surgical Resection Cancers (Basel), 2019;11(6):. 2019-01-01 [PMID: 31146405] (Neutralization, Human)
• C Ling, K Nishimoto, Z Rolfs, LM Smith, BL Frey, NV Welham Differentiated fibrocytes assume a functional mesenchymal phenotype with regenerative potential Sci Adv, 2019;5(5):eaav7384. 2019-01-01 [PMID: 31086819] (Bioassay, Rat)
• EP Reeves, DM Dunlea, K McQuillan, CA O'Dwyer, TP Carroll, R Saldova, PR Akepati, MR Wormald, OJ McElvaney, V Shutchaida, M Henry, P Meleady, J Keenan, DC Liberti, DN Kotton, PM Rudd, AA Wilson, NG McElvaney Circulating Truncated Alpha-1 Antitrypsin Glycoprotein in Patient Plasma Retains Anti-Inflammatory Capacity J. Immunol., 2019;0(0):. 2019-01-01 [PMID: 30796179] (Binding Assay, Human)
• CN Como, AN Bubak, AM Blackmon, D Jones, NH Mueller, R Davidson, MA Nagel Varicella Zoster Virus Induces Differential Cell-Type Specific Responses in Human Corneal Epithelial Cells and Keratocytes Invest. Ophthalmol. Vis. Sci., 2019;60(2):704-711. 2019-01-01 [PMID: 30786281] (Neutralization, Human)
• J Zhang, H Li, Q Wu, Y Chen, Y Deng, Z Yang, L Zhang, B Liu Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growth Redox Biol, 2019;22(0):101116. 2019-01-01 [PMID: 30769285] (Neutralization, Human)
• MJ Domínguez-, E Armas-Gonz, A Herrera-Ga, M Arce-Franc, M Feria, M Vicente-Ma, A Martínez-R, F Sánchez-Ma, F Díaz-Gonzá L-selectin expression is regulated by CXCL8-induced reactive oxygen species produced during human neutrophil rolling Eur. J. Immunol., 2018;0(0):. 2018-01-01 [PMID: 30443903] (Neutralization, Human)
• SE Logue, EP McGrath, P Cleary, S Greene, K Mnich, A Almanza, E Chevet, RM Dwyer, A Oommen, P Legembre, F Godey, EC Madden, B Leuzzi, J Obacz, Q Zeng, JB Patterson, R Jäger, AM Gorman, A Samali Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy Nat Commun, 2018;9(1):3267. 2018-01-01 [PMID: 30111846] (Neutralization, Human)
• B Gril, AN Paranjape, S Woditschka, E Hua, EL Dolan, J Hanson, X Wu, W Kloc, E Izycka-Swi, R Duchnowska, R P?ksa, W Biernat, J Jassem, N Nayyar, PK Brastianos, OM Hall, CJ Peer, WD Figg, GT Pauly, C Robinson, S Difilippan, E Bialecki, P Metellus, JP Schneider, PS Steeg Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases Psychoneuroendocrinology, 2018;9(1):2705. 2018-01-01 [PMID: 30006619] (Neutralization, Human)
• A Kumar, M Cherukumil, SH Mahmoudpou, K Brand, OR Bandapalli ShRNA-mediated knock-down of CXCL8 inhibits tumor growth in colorectal liver metastasis Biochem. Biophys. Res. Commun., 2018;0(0):. 2018-01-01 [PMID: 29679563] (Western Blot, Human)
• A Micera, A Di Zazzo, G Esposito, R Longo, W Foulsham, R Sacco, R Sgrulletta, S Bonini Age-Related Changes to Human Tear Composition Invest. Ophthalmol. Vis. Sci., 2018;59(5):2024-2031. 2018-01-01 [PMID: 29677365] (Western Blot, Human)
• Q Liu, A Li, S Yu, S Qin, N Han, RG Pestell, X Han, K Wu DACH1 antagonizes CXCL8 to repress tumorigenesis of lung adenocarcinoma and improve prognosis J Hematol Oncol, 2018;11(1):53. 2018-01-01 [PMID: 29636079] (Bioassay, Human)
• F Melo-Gonza, TM Fenton, C Forss, C Smedley, A Goenka, AS MacDonald, DJ Thornton, MA Travis Intestinal mucin activates human dendritic cells and IL-8 production in a glycan-specific manner J. Biol. Chem., 2018;0(0):. 2018-01-01 [PMID: 29581231] (Neutralization, Human)
• S Arl?er Endothelial cell leptin receptors, leptin and interleukin-8 in the pathogenesis of preeclampsia: An in-vitro study Turk J Obstet Gynecol, 2017;14(4):220-227. 2017-01-01 [PMID: 29379664] (Western Blot, Human)
• Y Uchino, AM Woodward, J Mauris, K Peterson, P Verma, UJ Nilsson, J Rajaiya, P Argüeso Galectin-3 is an amplifier of the interleukin-1?-mediated inflammatory response in corneal keratinocytes Immunology, 2018;0(0):. 2018-01-01 [PMID: 29359328] (Western Blot, Human)
• S di Martino, CA Amoreo, B Nuvoli, R Galati, S Strano, F Facciolo, G Alessandri, HI Pass, G Ciliberto, G Blandino, R De Maria, M Cioce HSP90 inhibition alters the chemotherapy-driven rearrangement of the oncogenic secretome Oncogene, 2018;0(0):. 2018-01-01 [PMID: 29311642] (Inhibition, Human)
• J Ikari, AJ Nelson, J Obaid, A Giron-Mart, K Ikari, F Makino, S Iwasawa, Y Gunji, M Farid, X Wang, H Basma, D Demeo, C Feghali-Bo, O Holz, K Rabe, X Liu, SI Rennard Reduced microRNA-503 expression augments lung fibroblast VEGF production in chronic obstructive pulmonary disease PLoS ONE, 2017;12(9):e0184039. 2017-01-01 [PMID: 28880936] (ELISA Development (Capture), Human)
• MM White, P Geraghty, E Hayes, S Cox, W Leitch, B Alfawaz, GM Lavelle, OJ McElvaney, R Flannery, J Keenan, P Meleady, M Henry, M Clynes, C Gunaratnam, NG McElvaney, EP Reeves Neutrophil Membrane Cholesterol Content is a Key Factor in Cystic Fibrosis Lung Disease EBioMedicine, 2017;23(0):173-184. 2017-01-01 [PMID: 28835336] (ELISA Development (Capture), Human)
• B Tian, A Al-Moujahe, P Bouzika, Y Hu, S Notomi, P Tsoka, JW Miller, H Lin, DG Vavvas Atorvastatin Promotes Phagocytosis and Attenuates Pro-Inflammatory Response in Human Retinal Pigment Epithelial Cells Sci Rep, 2017;7(1):2329. 2017-01-01 [PMID: 28539592] (Western Blot, Human)
• Y Wang, J Liu, Q Jiang, J Deng, F Xu, X Chen, F Cheng, Y Zhang, Y Yao, Z Xia, X Xu, X Su, M Huang, L Dai, Y Yang, S Zhang, D Yu, RC Zhao, Y Wei, H Deng Human adipose-derived mesenchymal stem cell-secreted CXCL1 and CXCL8 facilitate breast tumor growth by promoting angiogenesis Stem Cells, 2017;0(0):. 2017-01-01 [PMID: 28514506] (Neutralization, Human)
• J Liu, G Song, X Lin, X Pang, T Meng Upregulated unique long 16 binding protein 1 detected in preeclamptic placenta affects human extravillous trophoblast cell line (HTR-8/SVneo) invasion by modulating the function of uterine natural killer cells Exp Ther Med, 2017;13(4):1447-1455. 2017-01-01 [PMID: 28413491] (Functional Assay, Human)
• HB Song, HO Jun, JH Kim, YH Lee, MH Choi, JH Kim Disruption of outer blood-retinal barrier by Toxoplasma gondii-infected monocytes is mediated by paracrinely activated FAK signaling PLoS ONE, 2017;12(4):e0175159. 2017-01-01 [PMID: 28406972] (Neutralization, Human)
• CM Henry, SJ Martin Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory FADDosome Complex upon TRAIL Stimulation Mol. Cell, 2017;65(4):715-729.e5. 2017-01-01 [PMID: 28212752] (ICC, Human)
• HR Gatla, Y Zou, MM Uddin, B Singha, P Bu, A Vancura, I Vancurova Histone Deacetylase (HDAC) Inhibition Induces I?B Kinase (IKK)-dependent Interleukin-8/CXCL8 Expression in Ovarian Cancer Cells J. Biol. Chem., 2017;292(12):5043-5054. 2017-01-01 [PMID: 28167529] (Neutralization, Human)
• Human pancreatic cancer cells induce a MyD88-dependent stromal response to promote a tumor-tolerant immune microenvironment Cancer Res., 2016;0(0):. 2016-01-01 [PMID: 27864347] (Bioassay, Human)
• Yuh-Lin Wu Novel augmentation by bufalin of protein kinase C-induced cyclooxygenase-2 and IL-8 production in human breast cancer cells Innate Immun, 2016;0(0):. 2016-01-01 [PMID: 27821648] (Bioassay, Human)
• Growth Hormone Releasing Peptide-2 Attenuation of Protein Kinase C-Induced Inflammation in Human Ovarian Granulosa Cells Int J Mol Sci, 2016;17(8):. 2016-01-01 [PMID: 27548147] (Western Blot, Human)
• M Hoare, Y Ito, TW Kang, MP Weekes, NJ Matheson, DA Patten, S Shetty, AJ Parry, S Menon, R Salama, R Antrobus, K Tomimatsu, W Howat, PJ Lehner, L Zender, M Narita NOTCH1 mediates a switch between two distinct secretomes during senescence Nat. Cell Biol, 2016;18(9):979-92. 2016-01-01 [PMID: 27525720] (Western Blot, Human)
• Tsr Chemoreceptor Interacts With IL-8 Provoking E. coli Transmigration Across Human Lung Epithelial Cells Sci Rep, 2016;6(0):31087. 2016-01-01 [PMID: 27506372] (Immunoprecipitation, Bacteria - E. Coli)
• Z Jing, H Xu, X Chen, Q Zhong, J Huang, Y Zhang, W Guo, Z Yang, S Ding, P Chen, Z Huang The Proton-Sensing G-Protein Coupled Receptor GPR4 Promotes Angiogenesis in Head and Neck Cancer PLoS ONE, 2016;11(4):e0152789. 2016-01-01 [PMID: 27078157] (Neutralization, Human)
• Gao Y, Zhou Z, Lu S, Huang X, Zhang C, Jiang R, Yao A, Sun B, Wang X Chemokine CCL15 Mediates Migration of Human Bone Marrow-Derived Mesenchymal Stem Cells Toward Hepatocellular Carcinoma. Stem Cells, 0;34(4):1112-22. [PMID: 26763650] (Neutralization, Human)
• Chen L, Min L, Wang X, Zhao J, Chen H, Qin J, Chen W, Shen Z, Tang Z, Gan Q, Ruan Y, Sun Y, Qin X, Gu J Loss of RACK1 Promotes Metastasis of Gastric Cancer by Inducing a miR-302c/IL8 Signaling Loop. Cancer Res, 2015;75(18):3832-41. 2015-01-01 [PMID: 26199092] (Neutralization, Human)
• Takeuchi S, Baghdadi M, Tsuchikawa T, Wada H, Nakamura T, Abe H, Nakanishi S, Usui Y, Higuchi K, Takahashi M, Inoko K, Sato S, Takano H, Shichinohe T, Seino K, Hirano S Chemotherapy-Derived Inflammatory Responses Accelerate the Formation of Immunosuppressive Myeloid Cells in the Tissue Microenvironment of Human Pancreatic Cancer. Cancer Res, 2015;75(13):2629-40. 2015-01-01 [PMID: 25952647] (Neutralization, Human)
• McElvaney O, O'Reilly N, White M, Lacey N, Pohl K, Gerlza T, Bergin D, Kerr H, McCarthy C, O'Brien M, Adage T, Kungl A, Reeves E, McElvaney N The effect of the decoy molecule PA401 on CXCL8 levels in bronchoalveolar lavage fluid of patients with cystic fibrosis. Mol Immunol, 2015;63(2):550-8. 2015-01-01 [PMID: 25453468] (Bioassay, Human)
• Voltolini C, Battersby S, Novembri R, Torricelli M, Severi F, Petraglia F, Norman J Urocortin 2 role in placental and myometrial inflammatory mechanisms at parturition. Endocrinology, 2015;156(2):670-9. 2015-01-01 [PMID: 25426872] (ELISA Development (Capture), Human)
• Gouwy M, De Buck M, Portner N, Opdenakker G, Proost P, Struyf S, Van Damme J Serum amyloid A chemoattracts immature dendritic cells and indirectly provokes monocyte chemotaxis by induction of cooperating CC and CXC chemokines. Eur J Immunol, 2015;45(1):101-12. 2015-01-01 [PMID: 25345597] (Neutralization, Human)
• Kuravi S, McGettrick H, Satchell S, Saleem M, Harper L, Williams J, Rainger G, Savage C Podocytes regulate neutrophil recruitment by glomerular endothelial cells via IL-6-mediated crosstalk. J Immunol, 2014;193(1):234-43. 2014-01-01 [PMID: 24872191] (Neutralization, Human)
• Moelants , Eva A V, Loozen , Gitte, Mortier , Anneleen, Martens , Erik, Opdenakker , Ghislain, Mizgalska , Danuta, Szmigielski , Borys, Potempa , Jan, Van Damme , Jo, Teughels , Wim, Proost , Paul Citrullination and proteolytic processing of chemokines by Porphyromonas gingivalis. Infect Immun, 2014;82(6):2511-9. 2014-01-01 [PMID: 24686061] (ELISA Development (Detection), Bacteria - Porphyromonas gingivalis)
• Windheim M, Hansen B Interleukin-1-induced activation of the small GTPase Rac1 depends on receptor internalization and regulates gene expression. Cell Signal, 2014;26(1):49-55. 2014-01-01 [PMID: 24080160] (Western Blot, Human)
• Khanna , P, Chung , C-Y, Neves , R I, Robertson , G P, Dong , C CD82/KAI expression prevents IL-8-mediated endothelial gap formation in late-stage melanomas. Oncogene, 2013;33(22):2898-908. 2013-01-01 [PMID: 23873025] (ELISA Development (Capture), Human)
• Nakajima N, Van Tin N, Sato Y, Thach H, Katano H, Diep P, Kumasaka T, Thuy N, Hasegawa H, San L, Kawachi S, Liem N, Suzuki K, Sata T Pathological study of archival lung tissues from five fatal cases of avian H5N1 influenza in Vietnam. Mod Pathol, 2012;26(3):357-69. 2012-01-01 [PMID: 23174938] (IHC-P, Human)
• Macrophages induce differentiation of plasma cells through CXCL10/IP-10. J. Exp. Med., 2012;209(10):1813-23. 2012-01-01 [PMID: 22987802] (Neutralization, Human)
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Review for CXCL8/IL-8 Antibody (MAB208) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Felix Funck IHC-P Human 08/30/2018

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human lymph node (melanoma)
• Species: Human
• Lot: ASJ3517041

Comments

• Comments: Human FFPE metastatic melanoma lymph node sections were stained with anti-human IL-8 monoclonal antibody (MAB208) at 5 µg/ml followed by secondary goat anti-mouse biotinylated antibody and Streptavidin AP conjugate. Detection via an Alkaline Phosphatase/Red staining System.
  Blocking: 5 % goat Serum in TBS-T
  Antigen Retrieval: Citrat buffer
  Primary antibody incubation: over night at 4 °C

Bioinformatics

• Gene Symbol: IL8
• Uniprot:
  • P10145()