CXCL8/IL-8 Antibody (6217) [Unconjugated]

Images

 
IL-8/CXCL8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) at 10 µg/mL for 3 hours at room temperature. Cells ...read more
Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, CyTOF-ready, ELISA(Cap), Flow, ICC/IF
Clone
6217
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

CXCL8/IL-8 Antibody (6217) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IL-8/CXCL8
Ser28-Ser99
Accession # P10145
Specificity
Detects human IL-8/CXCL8 in ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant porcine IL-8/CXCL8 and no cross-reactivity with recombinant rat CXCL3/CINC-2 alpha is observed.
Details of Functionality
ActivityAssaywCitation not found None None None None None
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL8
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.08-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Human CXCL8/IL-8 Biotinylated Antibody (Catalog number BAF208)
Standard: Recombinant Human CXCL8/IL-8 (Catalog number 208-IL)
Publications
Read Publications using
MAB208 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL8/IL-8 Antibody (6217) [Unconjugated]

  • 3-10C
  • AMCF-I
  • C-X-C motif chemokine 8
  • CXCL8
  • CXCL8SCYB8
  • Emoctakin
  • GCP1
  • GCP-1TSG-1
  • IL8
  • IL-8
  • interleukin 8
  • K60
  • LAI
  • LECT
  • MDNCF
  • MDNCFb-ENAP
  • member 8
  • MONAPGCP1
  • NAP1
  • NAP-1NAP1
  • NCF
  • Neutrophil-activating protein 1
  • Protein 3-10C
  • T cell chemotactic factor
  • T-cell chemotactic factor
  • TCF
  • TSG1

Background

CXCL8, also known as IL-8, is a CXC chemokine that is a potent chemoattractant for neutrophils and has a wide range of other pro-inflammatory effects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56566
Species: Hu, Mu, Rt
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, CyTOF-ready, ELISA(Cap), Flow-CS, Flow-IC
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NB100-56722
Species: Hu, Ca
Applications: WB, Flow, ICC/IF, IHC-P, IP, B/N, Flow-CS

Publications for CXCL8/IL-8 Antibody (MAB208)(79)

We have publications tested in 6 confirmed species: Human, Bacteria - Porphyromonas gingivalis, Bovine, Complex Species Category, E. coli, Guinea Pig.

We have publications tested in 18 applications: Affinity Chromatography, Antibody Array Development, Array Development, Bioassay, ELISA Capture, ELISA Development, ELISA detection, Electrochemiluminescence, Functional Assay, ICC, IHC Fresh, IHC Frozen, IHC Paraffin-embedded, IP, Luminex Development, Neut, WB, inhibition.


Filter By Application
Affinity Chromatography
(1)
Antibody Array Development
(1)
Array Development
(1)
Bioassay
(4)
ELISA Capture
(4)
ELISA Development
(21)
ELISA detection
(1)
Electrochemiluminescence
(2)
Functional Assay
(1)
ICC
(6)
IHC Fresh
(1)
IHC Frozen
(1)
IHC Paraffin-embedded
(3)
IP
(1)
Luminex Development
(1)
Neut
(23)
WB
(6)
inhibition
(1)
All Applications
Filter By Species
Human
(74)
Bacteria - Porphyromonas gingivalis
(1)
Bovine
(1)
Complex Species Category
(1)
E. coli
(1)
Guinea Pig
(1)
All Species
Showing Publications 1 - 10 of 79. Show All 79 Publications.
Publications using MAB208 Applications Species
A Micera, A Di Zazzo, G Esposito, R Longo, W Foulsham, R Sacco, R Sgrulletta, S Bonini Age-Related Changes to Human Tear Composition Invest. Ophthalmol. Vis. Sci., 2018;59(5):2024-2031. 2018 [PMID: 29677365] (WB,, Human) WB Human
Q Liu, A Li, S Yu, S Qin, N Han, RG Pestell, X Han, K Wu DACH1 antagonizes CXCL8 to repress tumorigenesis of lung adenocarcinoma and improve prognosis J Hematol Oncol, 2018;11(1):53. 2018 [PMID: 29636079] (Bioassay, Human) Bioassay Human
F Melo-Gonza, TM Fenton, C Forss, C Smedley, A Goenka, AS MacDonald, DJ Thornton, MA Travis Intestinal mucin activates human dendritic cells and IL-8 production in a glycan-specific manner J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29581231] (Neut,, Human) Neut Human
S di Martino, CA Amoreo, B Nuvoli, R Galati, S Strano, F Facciolo, G Alessandri, HI Pass, G Ciliberto, G Blandino, R De Maria, M Cioce HSP90 inhibition alters the chemotherapy-driven rearrangement of the oncogenic secretome Oncogene, 2018;0(0):. 2018 [PMID: 29311642] (inhibition, Human) inhibition Human
J Ikari, AJ Nelson, J Obaid, A Giron-Mart, K Ikari, F Makino, S Iwasawa, Y Gunji, M Farid, X Wang, H Basma, D Demeo, C Feghali-Bo, O Holz, K Rabe, X Liu, SI Rennard Reduced microRNA-503 expression augments lung fibroblast VEGF production in chronic obstructive pulmonary disease PLoS ONE, 2017;12(9):e0184039. 2017 [PMID: 28880936] (ELISA Capture,, Human) ELISA Capture Human
MM White, P Geraghty, E Hayes, S Cox, W Leitch, B Alfawaz, GM Lavelle, OJ McElvaney, R Flannery, J Keenan, P Meleady, M Henry, M Clynes, C Gunaratnam, NG McElvaney, EP Reeves Neutrophil Membrane Cholesterol Content is a Key Factor in Cystic Fibrosis Lung Disease EBioMedicine, 2017;23(0):173-184. 2017 [PMID: 28835336] (ELISA Capture,, Human) ELISA Capture Human
B Tian, A Al-Moujahe, P Bouzika, Y Hu, S Notomi, P Tsoka, JW Miller, H Lin, DG Vavvas Atorvastatin Promotes Phagocytosis and Attenuates Pro-Inflammatory Response in Human Retinal Pigment Epithelial Cells Sci Rep, 2017;7(1):2329. 2017 [PMID: 28539592] (WB,, Human) WB Human
Y Wang, J Liu, Q Jiang, J Deng, F Xu, X Chen, F Cheng, Y Zhang, Y Yao, Z Xia, X Xu, X Su, M Huang, L Dai, Y Yang, S Zhang, D Yu, RC Zhao, Y Wei, H Deng Human adipose-derived mesenchymal stem cell-secreted CXCL1 and CXCL8 facilitate breast tumor growth by promoting angiogenesis Stem Cells, 2017;0(0):. 2017 [PMID: 28514506] (Neut,, Human) Neut Human
J Liu, G Song, X Lin, X Pang, T Meng Upregulated unique long 16 binding protein 1 detected in preeclamptic placenta affects human extravillous trophoblast cell line (HTR-8/SVneo) invasion by modulating the function of uterine natural killer cells Exp Ther Med, 2017;13(4):1447-1455. 2017 [PMID: 28413491] (Functional Assay,, Human) Functional Assay Human
HB Song, HO Jun, JH Kim, YH Lee, MH Choi, JH Kim Disruption of outer blood-retinal barrier by Toxoplasma gondii-infected monocytes is mediated by paracrinely activated FAK signaling PLoS ONE, 2017;12(4):e0175159. 2017 [PMID: 28406972] (Neut,, Human) Neut Human
Show All 79 Publications.

Reviews for CXCL8/IL-8 Antibody (MAB208) (0)

There are no reviews for CXCL8/IL-8 Antibody (MAB208). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CXCL8/IL-8 Antibody (MAB208) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for CXCL8/IL-8 Antibody (MAB208)

Discover related pathways, diseases and genes to CXCL8/IL-8 Antibody (MAB208). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CXCL8/IL-8 Antibody (MAB208)

Discover more about diseases related to CXCL8/IL-8 Antibody (MAB208).
 

Pathways for CXCL8/IL-8 Antibody (MAB208)

View related products by pathway.

PTMs for CXCL8/IL-8 Antibody (MAB208)

Learn more about PTMs related to CXCL8/IL-8 Antibody (MAB208).

Blogs on CXCL8/IL-8

There are no specific blogs for CXCL8/IL-8, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CXCL8/IL-8 Antibody (6217) [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol IL8
Entrez
Uniprot