CXCL8/IL-8 Antibody (6217) [Unconjugated]

Images

 
Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more
IL-8/CXCL8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) at 10 µg/mL for 3 hours at room temperature. Cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, CyTOF-ready, ELISA(Cap), ICC, ICFlow, Neut
Clone
6217
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

CXCL8/IL-8 Antibody (6217) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IL-8/CXCL8
Ser28-Ser99
Accession # P10145
Specificity
Detects human IL-8/CXCL8 in ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant porcine IL-8/CXCL8 and no cross-reactivity with recombinant rat CXCL3/CINC-2 alpha is observed.
Details of Functionality
ActivityAssaywCitation not found None None None None None
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL8
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.08-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Human CXCL8/IL-8 Biotinylated Antibody (Catalog number BAF208)
Standard: Recombinant Human CXCL8/IL-8 (Catalog number 208-IL)
Publications
Read Publications using
MAB208 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL8/IL-8 Antibody (6217) [Unconjugated]

  • 3-10C
  • AMCF-I
  • C-X-C motif chemokine 8
  • CXCL8
  • CXCL8SCYB8
  • Emoctakin
  • GCP1
  • GCP-1TSG-1
  • IL8
  • IL-8
  • interleukin 8
  • K60
  • LAI
  • LECT
  • MDNCF
  • MDNCFb-ENAP
  • member 8
  • MONAPGCP1
  • NAP1
  • NAP-1NAP1
  • NCF
  • Neutrophil-activating protein 1
  • Protein 3-10C
  • T cell chemotactic factor
  • T-cell chemotactic factor
  • TCF
  • TSG1

Background

CXCL8, also known as IL-8, is a CXC chemokine that is a potent chemoattractant for neutrophils and has a wide range of other pro-inflammatory effects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCL8/IL-8 Antibody (MAB208)(70)

We have publications tested in 6 confirmed species: Human, Bacteria - Porphyromonas gingivalis, Bovine, Complex Species Category, E. coli, Guinea Pig.

We have publications tested in 16 applications: Affinity Chromatography, Antibody Array Development, Array Development, Bioassay, ELISA Capture, ELISA Development, ELISA detection, Electrochemiluminescence, ICC, IHC Fresh, IHC Frozen, IHC Paraffin-embedded, IP, Luminex Development, Neut, WB.


Filter By Application
Affinity Chromatography
(1)
Antibody Array Development
(1)
Array Development
(1)
Bioassay
(2)
ELISA Capture
(4)
ELISA Development
(21)
ELISA detection
(1)
Electrochemiluminescence
(2)
ICC
(5)
IHC Fresh
(1)
IHC Frozen
(1)
IHC Paraffin-embedded
(3)
IP
(1)
Luminex Development
(1)
Neut
(20)
WB
(5)
All Applications
Filter By Species
Human
(65)
Bacteria - Porphyromonas gingivalis
(1)
Bovine
(1)
Complex Species Category
(1)
E. coli
(1)
Guinea Pig
(1)
All Species
Showing Publications 1 - 10 of 70. Show All 70 Publications.
Publications using MAB208 Applications Species
J Ikari, AJ Nelson, J Obaid, A Giron-Mart, K Ikari, F Makino, S Iwasawa, Y Gunji, M Farid, X Wang, H Basma, D Demeo, C Feghali-Bo, O Holz, K Rabe, X Liu, SI Rennard Reduced microRNA-503 expression augments lung fibroblast VEGF production in chronic obstructive pulmonary disease PLoS ONE, 2017;12(9):e0184039. 2017 [PMID: 28880936] (ELISA Capture,, Human) ELISA Capture Human
MM White, P Geraghty, E Hayes, S Cox, W Leitch, B Alfawaz, GM Lavelle, OJ McElvaney, R Flannery, J Keenan, P Meleady, M Henry, M Clynes, C Gunaratnam, NG McElvaney, EP Reeves Neutrophil Membrane Cholesterol Content is a Key Factor in Cystic Fibrosis Lung Disease EBioMedicine, 2017;23(0):173-184. 2017 [PMID: 28835336] (ELISA Capture,, Human) ELISA Capture Human
B Tian, A Al-Moujahe, P Bouzika, Y Hu, S Notomi, P Tsoka, JW Miller, H Lin, DG Vavvas Atorvastatin Promotes Phagocytosis and Attenuates Pro-Inflammatory Response in Human Retinal Pigment Epithelial Cells Sci Rep, 2017;7(1):2329. 2017 [PMID: 28539592] (WB,, Human) WB Human
Y Wang, J Liu, Q Jiang, J Deng, F Xu, X Chen, F Cheng, Y Zhang, Y Yao, Z Xia, X Xu, X Su, M Huang, L Dai, Y Yang, S Zhang, D Yu, RC Zhao, Y Wei, H Deng Human adipose-derived mesenchymal stem cell-secreted CXCL1 and CXCL8 facilitate breast tumor growth by promoting angiogenesis Stem Cells, 2017;0(0):. 2017 [PMID: 28514506] (Neut,, Human) Neut Human
HB Song, HO Jun, JH Kim, YH Lee, MH Choi, JH Kim Disruption of outer blood-retinal barrier by Toxoplasma gondii-infected monocytes is mediated by paracrinely activated FAK signaling PLoS ONE, 2017;12(4):e0175159. 2017 [PMID: 28406972] (Neut,, Human) Neut Human
Human pancreatic cancer cells induce a MyD88-dependent stromal response to promote a tumor-tolerant immune microenvironment Cancer Res., 2016;0(0):. 2016 [PMID: 27864347] (Bioassay,, Human) Bioassay Human
Growth Hormone Releasing Peptide-2 Attenuation of Protein Kinase C-Induced Inflammation in Human Ovarian Granulosa Cells Int J Mol Sci, 2016;17(8):. 2016 [PMID: 27548147] (WB,, Human) WB Human
M Hoare, Y Ito, TW Kang, MP Weekes, NJ Matheson, DA Patten, S Shetty, AJ Parry, S Menon, R Salama, R Antrobus, K Tomimatsu, W Howat, PJ Lehner, L Zender, M Narita NOTCH1 mediates a switch between two distinct secretomes during senescence Nat. Cell Biol, 2016;18(9):979-92. 2016 [PMID: 27525720] (WB,, Human) WB Human
Tsr Chemoreceptor Interacts With IL-8 Provoking E. coli Transmigration Across Human Lung Epithelial Cells Sci Rep, 2016;6(0):31087. 2016 [PMID: 27506372] (IP,, E. coli) IP E. coli
Z Jing, H Xu, X Chen, Q Zhong, J Huang, Y Zhang, W Guo, Z Yang, S Ding, P Chen, Z Huang The Proton-Sensing G-Protein Coupled Receptor GPR4 Promotes Angiogenesis in Head and Neck Cancer PLoS ONE, 2016;11(4):e0152789. 2016 [PMID: 27078157] (Neut,, Human) Neut Human
Show All 70 Publications.

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Bioinformatics

Gene Symbol IL8
Entrez
Uniprot