CXCL8/IL-8 Antibody (6217) [Unconjugated]

Images

 
Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more
IL-8/CXCL8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) at 10 µg/mL for 3 hours at room temperature. Cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, CyTOF-ready, ELISA(Cap), Flow, ICC/IF
Clone
6217
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

CXCL8/IL-8 Antibody (6217) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IL-8/CXCL8
Ser28-Ser99
Accession # P10145
Specificity
Detects human IL-8/CXCL8 in ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant porcine IL-8/CXCL8 and no cross-reactivity with recombinant rat CXCL3/CINC-2 alpha is observed.
Details of Functionality
ActivityAssaywCitation not found None None None None None
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL8
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.08-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Human CXCL8/IL-8 Biotinylated Antibody (Catalog number BAF208)
Standard: Recombinant Human CXCL8/IL-8 (Catalog number 208-IL)
Reviewed Applications
Read 1 Review rated 4
using
MAB208 in the following applications:

Publications
Read Publications using
MAB208 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL8/IL-8 Antibody (6217) [Unconjugated]

  • 3-10C
  • AMCF-I
  • C-X-C motif chemokine 8
  • CXCL8
  • CXCL8SCYB8
  • Emoctakin
  • GCP1
  • GCP-1TSG-1
  • IL8
  • IL-8
  • interleukin 8
  • K60
  • LAI
  • LECT
  • MDNCF
  • MDNCFb-ENAP
  • member 8
  • MONAPGCP1
  • NAP1
  • NAP-1NAP1
  • NCF
  • Neutrophil-activating protein 1
  • Protein 3-10C
  • T cell chemotactic factor
  • T-cell chemotactic factor
  • TCF
  • TSG1

Background

CXCL8, also known as IL-8, is a CXC chemokine that is a potent chemoattractant for neutrophils and has a wide range of other pro-inflammatory effects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu
Applications: WB, B/N, CyTOF-ready, ELISA(Cap), Flow, ICC/IF

Publications for CXCL8/IL-8 Antibody (MAB208)(81)

We have publications tested in 6 confirmed species: Human, Bacteria - Porphyromonas gingivalis, Bovine, Complex Species Category, E. coli, Guinea Pig.

We have publications tested in 18 applications: Affinity Chromatography, Antibody Array Development, Array Development, Bioassay, ELISA Capture, ELISA Development, ELISA detection, Electrochemiluminescence, Functional Assay, ICC, IHC Fresh, IHC Frozen, IHC Paraffin-embedded, IP, Luminex Development, Neut, WB, inhibition.


Filter By Application
Affinity Chromatography
(1)
Antibody Array Development
(1)
Array Development
(1)
Bioassay
(4)
ELISA Capture
(4)
ELISA Development
(21)
ELISA detection
(1)
Electrochemiluminescence
(2)
Functional Assay
(1)
ICC
(6)
IHC Fresh
(1)
IHC Frozen
(1)
IHC Paraffin-embedded
(3)
IP
(1)
Luminex Development
(1)
Neut
(25)
WB
(6)
inhibition
(1)
All Applications
Filter By Species
Human
(76)
Bacteria - Porphyromonas gingivalis
(1)
Bovine
(1)
Complex Species Category
(1)
E. coli
(1)
Guinea Pig
(1)
All Species
Showing Publications 1 - 10 of 81. Show All 81 Publications.
Publications using MAB208 Applications Species
SE Logue, EP McGrath, P Cleary, S Greene, K Mnich, A Almanza, E Chevet, RM Dwyer, A Oommen, P Legembre, F Godey, EC Madden, B Leuzzi, J Obacz, Q Zeng, JB Patterson, R Jäger, AM Gorman, A Samali Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy Nat Commun, 2018;9(1):3267. 2018 [PMID: 30111846] (Neut,, Human) Neut Human
B Gril, AN Paranjape, S Woditschka, E Hua, EL Dolan, J Hanson, X Wu, W Kloc, E Izycka-Swi, R Duchnowska, R P?ksa, W Biernat, J Jassem, N Nayyar, PK Brastianos, OM Hall, CJ Peer, WD Figg, GT Pauly, C Robinson, S Difilippan, E Bialecki, P Metellus, JP Schneider, PS Steeg Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases Psychoneuroendocrinology, 2018;9(1):2705. 2018 [PMID: 30006619] (Neut,, Human) Neut Human
A Micera, A Di Zazzo, G Esposito, R Longo, W Foulsham, R Sacco, R Sgrulletta, S Bonini Age-Related Changes to Human Tear Composition Invest. Ophthalmol. Vis. Sci., 2018;59(5):2024-2031. 2018 [PMID: 29677365] (WB,, Human) WB Human
Q Liu, A Li, S Yu, S Qin, N Han, RG Pestell, X Han, K Wu DACH1 antagonizes CXCL8 to repress tumorigenesis of lung adenocarcinoma and improve prognosis J Hematol Oncol, 2018;11(1):53. 2018 [PMID: 29636079] (Bioassay, Human) Bioassay Human
F Melo-Gonza, TM Fenton, C Forss, C Smedley, A Goenka, AS MacDonald, DJ Thornton, MA Travis Intestinal mucin activates human dendritic cells and IL-8 production in a glycan-specific manner J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29581231] (Neut,, Human) Neut Human
S di Martino, CA Amoreo, B Nuvoli, R Galati, S Strano, F Facciolo, G Alessandri, HI Pass, G Ciliberto, G Blandino, R De Maria, M Cioce HSP90 inhibition alters the chemotherapy-driven rearrangement of the oncogenic secretome Oncogene, 2018;0(0):. 2018 [PMID: 29311642] (inhibition, Human) inhibition Human
J Ikari, AJ Nelson, J Obaid, A Giron-Mart, K Ikari, F Makino, S Iwasawa, Y Gunji, M Farid, X Wang, H Basma, D Demeo, C Feghali-Bo, O Holz, K Rabe, X Liu, SI Rennard Reduced microRNA-503 expression augments lung fibroblast VEGF production in chronic obstructive pulmonary disease PLoS ONE, 2017;12(9):e0184039. 2017 [PMID: 28880936] (ELISA Capture,, Human) ELISA Capture Human
MM White, P Geraghty, E Hayes, S Cox, W Leitch, B Alfawaz, GM Lavelle, OJ McElvaney, R Flannery, J Keenan, P Meleady, M Henry, M Clynes, C Gunaratnam, NG McElvaney, EP Reeves Neutrophil Membrane Cholesterol Content is a Key Factor in Cystic Fibrosis Lung Disease EBioMedicine, 2017;23(0):173-184. 2017 [PMID: 28835336] (ELISA Capture,, Human) ELISA Capture Human
B Tian, A Al-Moujahe, P Bouzika, Y Hu, S Notomi, P Tsoka, JW Miller, H Lin, DG Vavvas Atorvastatin Promotes Phagocytosis and Attenuates Pro-Inflammatory Response in Human Retinal Pigment Epithelial Cells Sci Rep, 2017;7(1):2329. 2017 [PMID: 28539592] (WB,, Human) WB Human
Y Wang, J Liu, Q Jiang, J Deng, F Xu, X Chen, F Cheng, Y Zhang, Y Yao, Z Xia, X Xu, X Su, M Huang, L Dai, Y Yang, S Zhang, D Yu, RC Zhao, Y Wei, H Deng Human adipose-derived mesenchymal stem cell-secreted CXCL1 and CXCL8 facilitate breast tumor growth by promoting angiogenesis Stem Cells, 2017;0(0):. 2017 [PMID: 28514506] (Neut,, Human) Neut Human
Show All 81 Publications.

Review for CXCL8/IL-8 Antibody (MAB208) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB208:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin CXCL8/IL-8 MAB208
Enlarge
reviewed by:
Anonymous
IHC-P Human 08/30/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman lymph node (melanoma)
SpeciesHuman
LotASJ3517041

Comments

CommentsHuman FFPE metastatic melanoma lymph node sections were stained with anti-human IL-8 monoclonal antibody &#40;MAB208&#41; at 5 µg/ml followed by secondary goat anti-mouse biotinylated antibody and Streptavidin AP conjugate. Detection via an Alkaline Phosphatase/Red staining System.
Blocking: 5 % goat Serum in TBS-T
Antigen Retrieval: Citrat buffer
Primary antibody incubation: over night at 4 °C

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Recent Reviews

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1
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Anonymous
08/30/2018
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol IL8
Uniprot