TLR5 Antibody (19D759.2)


Western Blot: TLR5 Antibody (19D759.2) [NBP2-24787] - BEAS-2B cells whole cell lysate. Image from verified customer review.
Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) [NBP2-24787] - analysis of TLR5 in formalin-fixed, paraffin-embedded mouse brain tissue using TLR5 antibody at 5 ug/ml.
Biological Strategies: Flow Cytometry: TLR5 Antibody (19D759.2) [NBP2-24787] - Analysis using the PE conjugate of NBP2-24787. Staining of CD14-/CD83+/TLR5+ diffferentiated myeloid dendritic cells (mDCs). PBMCs more
Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) [NBP2-24787] - Formalin-fixed, paraffin-embedded human skin stained with TLR5 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.
Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) [NBP2-24787] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human skin using 5 ug/ml concentration of TLR5 antibody (clone 19D759.2). more
Flow Cytometry: TLR5 Antibody (19D759.2) [NBP2-24787] - Intracellular flow analysis of TLR5 in human monocytes using 0.5 ug of TLR5 antibody. Shaded histogram represents cells without antibody; green represents isotype more
Flow Cytometry: TLR5 Antibody (19D759.2) [NBP2-24787] - Analysis using the FITC conjugate of NBP2-24787. Staining of TLR5 in PBMCs using 0.5 ug/10^6 cells of this antibody. Shaded histogram represents cells without more
Flow Cytometry: TLR5 Antibody (19D759.2) [NBP2-24787] - Analysis using the PE conjugate of NBP2-24787. Staining of TLR5 in human PBMCs using 1 ug/10^6 cells of this antibody. Shaded histogram represents cells without more

Product Details

Reactivity Hu, Mu, CaSpecies Glossary
Applications WB, Flow, Flow-CS, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP
1.0 mg/ml
Validated by:

Biological Strategies


TLR5 Antibody (19D759.2) Summary

This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of human TLR5 between amino acids 700-800. The antibody also reacts with mouse TLR5.
IgG2a Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1-3 ug/ml
  • Flow Cytometry 0.5-1 ug/10^6 cells
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 10 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation
  • Flow (Cell Surface)
  • Flow (Intracellular)
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Use in Flow-cell surface and Flow-intracellular reported in scientific literature (PMID 24412598). Use in Immunohistochemistry reported in scientific literature (PMID 27392931).
Read Publications using
NBP2-24787 in the following applications:

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for TLR5 Antibody (19D759.2)

  • MGC126430
  • MGC126431
  • SLEB1FLJ10052
  • TIL-3
  • TIL3Toll/interleukin-1 receptor-like protein 3
  • TLR5
  • toll-like receptor 5


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: Flow, IHC, IHC-P, Flow-CS, Flow-IC
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Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Flow-IC, IF
Species: Hu
Species: Hu, Mu, Rt, Mk
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Applications: WB, Flow, Flow-CS, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP
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Publications for TLR5 Antibody (NBP2-24787)(29)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 8 applications: DB, Flow, Flow-CS, Flow-IC, ICC/IF, IHC, IHC-P, WB.

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Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using NBP2-24787 Applications Species
Kylma AK, Jouhi L, Listyarifah D et al. Treponema denticola chymotrypsin-like protease as associated with HPV-negative oropharyngeal squamous cell carcinoma. Br. J. Cancer. 43273 [PMID: 29930251] (IHC, Human) IHC Human
Uchida M, Oyanagi E, Kawanishi N et al. Exhaustive exercise increases the TNF-a production in response to flagellin via the upregulation of toll-like receptor 5 in the large intestine in mice Immunol Lett (IHC-P, Flow, Flow-CS, Human, Mouse)

This publication used the FITC conjugated form of this antibody (Cat# NBP2-24784).
IHC-P, Flow, Flow-CS Human, Mouse
Larsson O, Tengroth L, Xu Y et al. Substance P represents a novel first-line defense mechanism in the nose. J. Allergy Clin. Immunol. 2017 Feb 20 [PMID: 28219705] (Flow, Mouse) Flow Mouse
Huhta H, Helminen O, Kauppila JH et al. The Expression of Toll-like Receptors in Normal Human and Murine Gastrointestinal Organs and the Effect of Microbiome and Cancer J. Histochem. Cytochem. 2016 Aug 01 [PMID: 27370795] (IHC-P, Human) IHC-P Human
Carevic M, Oz H, Fuchs K et al. CXCR1 Regulates Pulmonary Anti-Pseudomonas Host Defense. J Innate Immun. 2016 Mar 08 [PMID: 26950764]

This citation used the FITC version of this antibody.
Makinen LK, Atula T, Hayry V et al. Predictive role of toll-like receptors 2, 4, and 9 in oral tongue squamous cell carcinoma. Oral Oncol. 2014 Sep 25 [PMID: 25264223] (IHC-P, Human)

TLR5 antibody used at 1:200 dilution for IHC-P on oral tongue squamous cell carcinoma tissues / OTSCC (Stage I-II patients) - TMA paraffin sections, antigen retrieval with PreTreatment-module in Tris-HCl buffer pH 8.5 at 98C for 20 min, Autostainer based assay with 1 hour primary incubation time followed by detection using REAL EnVision/HRP-DAB system (Fig 1, Cytoplasmic staining pattern).
IHC-P Human
Makinen, LK. Matrix metalloproteinases and toll-like receptors in early-stage oral tongue squamous cell carcinoma Thesis. 2015 (IHC-P, Human) IHC-P Human
Helminen O, Huhta H, Leppanen J et al. Nuclear localization of Toll-like receptor 5 in Barrett's esophagus and esophageal adenocarcinoma is associated with metastatic behavior Virchows Arch. 2016 Jul 09 [PMID: 27392931] (IHC, Human) IHC Human
Jeon YK, Kim CK, Koh J et al. Pellino-1 confers chemoresistance in lung cancer cells by upregulating cIAP2 through Lys63-mediated polyubiquitination. Oncotarget. Jul 5 2016 [PMID: 27248820] (DB, Human) DB Human
Renkonen J, Toppila-Salmi S, Joenvaara S et al. Expression of Toll-like receptors in nasal epithelium in allergic rhinitis APMIS. 2015 Jun 08 [PMID: 26061394] (IHC-P, Human)

TLR5 antibody (Imgenex IMG-664A) was used for IHC-P on nasal biopsies from healthy non-smoking volunteers both allergic to birch pollen and non-allergic controls. Nasal biopsies were collected before and after off-seasonal intranasal birch pollen or diluent challenge and the immunostaining assay implicated - 4um paraffin sections, antigen retrieval withTris-HCl pH 8.5, processing on Autostainer, use of primary at 2.5 ug/mL concentration, Rabbit/Mouse REAL EnVision Detection system and Peroxidase/DAB+ detection (Fig 1, Fig 2D ).
IHC-P Human
Show All 29 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TLR5 Antibody (NBP2-24787) (0)

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-24787AF405
Alexa Fluor 488 Labeled NBP2-24787AF488
Alexa Fluor 647 Labeled NBP2-24787AF647
Alexa Fluor 700 Labeled NBP2-24787AF700
Allophycocyanin Labeled NBP2-24787APC
Biotin Labeled NBP2-24787B
DyLight 350 Labeled NBP2-24787UV
DyLight 405 Labeled NBP2-24787V
DyLight 488 Labeled NBP2-24787G
DyLight 550 Labeled NBP2-24787R
DyLight 650 Labeled NBP2-24787C
DyLight 680 Labeled NBP2-24787FR
DyLight 755 Labeled NBP2-24787IR
HRP Labeled NBP2-24787H
PE Labeled NBP2-24783
PerCP Labeled NBP2-24787PCP

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Blogs on TLR5.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

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Gene Symbol TLR5