TLR5 Antibody (19D759.2)

Images

 
Western Blot: TLR5 Antibody (19D759.2) [NBP2-24787] - BEAS-2B cells whole cell lysate. Image from verified customer review.
Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) [NBP2-24787] - analysis of TLR5 in formalin-fixed, paraffin-embedded mouse brain tissue using TLR5 antibody at 5 ug/ml.
Flow Cytometry: TLR5 Antibody (19D759.2) [NBP2-24787] - Analysis using the PE conjugate of NBP2-24787. Staining of CD14-/CD83+/TLR5+ diffferentiated myeloid dendritic cells (mDCs). PBMCs were stimulated 5 days with ...read more
Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) [NBP2-24787] - Formalin-fixed, paraffin-embedded human skin stained with TLR5 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.
Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) [NBP2-24787] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human skin using 5 ug/ml concentration of TLR5 antibody (clone 19D759.2). ...read more
Flow Cytometry: TLR5 Antibody (19D759.2) [NBP2-24787] - Intracellular flow analysis of TLR5 in human monocytes using 0.5 ug of TLR5 antibody. Shaded histogram represents cells without antibody; green represents isotype ...read more
Flow Cytometry: TLR5 Antibody (19D759.2) [NBP2-24787] - Analysis using the FITC conjugate of NBP2-24787. Staining of TLR5 in PBMCs using 0.5 ug/10^6 cells of this antibody. Shaded histogram represents cells without ...read more
Flow Cytometry: TLR5 Antibody (19D759.2) [NBP2-24787] - Analysis using the PE conjugate of NBP2-24787. Staining of TLR5 in human PBMCs using 1 ug/10^6 cells of this antibody. Shaded histogram represents cells without ...read more

Product Details

Summary
Reactivity Hu, Mu, CaSpecies Glossary
Applications WB, Flow, Flow-CS, Flow-IC, IHC, IHC-Fr, IHC-P
Clone
19D759.2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

TLR5 Antibody (19D759.2) Summary

Immunogen
This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of human TLR5 between amino acids 700-800. The antibody also reacts with mouse TLR5.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TLR5
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Flow Cytometry 0.5-1 ug/10^6 cells
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 10 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Flow (Cell Surface)
  • Flow (Intracellular)
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Use in Flow-cell surface and Flow-intracellular reported in scientific literature (PMID 24412598). Use in Immunohistochemistry reported in scientific literature (PMID 27392931).
Agonist
Publications
Read Publications using
NBP2-24787 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TLR5 Antibody (19D759.2)

  • MGC126430
  • MGC126431
  • SLEB1FLJ10052
  • TIL-3
  • TIL3Toll/interleukin-1 receptor-like protein 3
  • TLR5
  • toll-like receptor 5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56566
Species: Hu, Mu, Rt
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, CyTOF-ready, Flow-CS, Flow-IC
NB100-56722
Species: Hu, Ca
Applications: WB, B/N, Flow, Func, ICC/IF, IHC-P, IP, Flow-CS
NBP2-24875
Species: Hu, Mu, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
NBP2-24729
Species: Hu, Mu, Rt, Ca, Eq, Pm
Applications: WB, Simple Western, ELISA, Flow, Func, ICC/IF, IHC, IHC-P, IP, In vitro, CyTOF-ready, Flow-IC
NBP2-24906
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Flow-IC, IF
NB100-56563
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P
MAB208
Species: Hu
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
NBP2-29323
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
NBP2-29328
Species: Hu, Mu, Rt, Xp, Ye, Ze
Applications: ELISA
NB100-56536
Species: Hu
Applications: Flow, IHC, IHC-P, Flow-CS, Flow-IC
NBP2-24917
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC-P, CyTOF-ready, Flow-IC
DDX0490P-100
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Flow-IC
NBP1-88968
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-24787
Species: Hu, Mu, Ca
Applications: WB, Flow, Flow-CS, Flow-IC, IHC, IHC-Fr, IHC-P
Supplier Logo

Publications for TLR5 Antibody (NBP2-24787)(28)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 8 applications: DB, Flow, Flow-CS, Flow-IC, ICC/IF, IHC, IHC-P, WB.


Filter By Application
DB
(1)
Flow
(2)
Flow-CS
(4)
Flow-IC
(2)
ICC/IF
(1)
IHC
(2)
IHC-P
(11)
WB
(4)
All Applications
Filter By Species
Human
(15)
Mouse
(5)
All Species
Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using NBP2-24787 Applications Species
Larsson O, Tengroth L, Xu Y et al. Substance P represents a novel first-line defense mechanism in the nose. J. Allergy Clin. Immunol. 2017 Feb 20 [PMID: 28219705] (Flow, Mouse) Flow Mouse
Helminen O, Huhta H, Leppanen J et al. Nuclear localization of Toll-like receptor 5 in Barrett's esophagus and esophageal adenocarcinoma is associated with metastatic behavior Virchows Arch. 2016 Jul 09 [PMID: 27392931] (IHC, Human) IHC Human
Huhta H, Helminen O, Kauppila JH et al. The Expression of Toll-like Receptors in Normal Human and Murine Gastrointestinal Organs and the Effect of Microbiome and Cancer J. Histochem. Cytochem. 2016 Aug 01 [PMID: 27370795] (IHC-P, Human) IHC-P Human
Jeon YK, Kim CK, Koh J et al. Pellino-1 confers chemoresistance in lung cancer cells by upregulating cIAP2 through Lys63-mediated polyubiquitination. Oncotarget. Jul 5 2016 [PMID: 27248820] (DB, Human) DB Human
Carevic M, Oz H, Fuchs K et al. CXCR1 Regulates Pulmonary Anti-Pseudomonas Host Defense. J Innate Immun. 2016 Mar 08 [PMID: 26950764]

Details:
This citation used the FITC version of this antibody.
Renkonen J, Toppila-Salmi S, Joenvaara S et al. Expression of Toll-like receptors in nasal epithelium in allergic rhinitis APMIS. 2015 Jun 08 [PMID: 26061394] (IHC-P, Human)

Details:
TLR5 antibody (Imgenex IMG-664A) was used for IHC-P on nasal biopsies from healthy non-smoking volunteers both allergic to birch pollen and non-allergic controls. Nasal biopsies were collected before and after off-seasonal intranasal birch pollen or diluent challenge and the immunostaining assay implicated - 4um paraffin sections, antigen retrieval withTris-HCl pH 8.5, processing on Autostainer, use of primary at 2.5 ug/mL concentration, Rabbit/Mouse REAL EnVision Detection system and Peroxidase/DAB+ detection (Fig 1, Fig 2D ).
IHC-P Human
Jouhi L, Datta N, Renkonen S et al. Expression of toll-like receptors in HPV-positive and HPV-negative oropharyngeal squamous cell carcinoma-an in vivo and in vitro study. Tumour Biol. 2015 May 05 [PMID: 25941114] (IHC-P, Human)

Details:
TLR5 antibody was used for IHC-P analysis of formalin-fixed paraffin-embedded sections of human HPV-positive and HPV-negative oropharyngeal squamous cell carcinoma tissues.
IHC-P Human
Makinen LK, Atula T, Hayry V et al. Predictive role of toll-like receptors 2, 4, and 9 in oral tongue squamous cell carcinoma. Oral Oncol. 2014 Sep 25 [PMID: 25264223] (IHC-P, Human)

Details:
TLR5 antibody used at 1:200 dilution for IHC-P on oral tongue squamous cell carcinoma tissues / OTSCC (Stage I-II patients) - TMA paraffin sections, antigen retrieval with PreTreatment-module in Tris-HCl buffer pH 8.5 at 98C for 20 min, Autostainer based assay with 1 hour primary incubation time followed by detection using REAL EnVision/HRP-DAB system (Fig 1, Cytoplasmic staining pattern).
IHC-P Human
Uchida M, Oyanagi E, Kawanishi N et al. Exhaustive exercise increases the TNF-a production in response to flagellin via the upregulation of toll-like receptor 5 in the large intestine in mice Immunol Lett 2014 Mar 3 [PMID: 24412598] (IHC-P, Flow-IC, Flow-CS, Human, Mouse)

Details:
This citation used the FITC version of this antibody.
IHC-P, Flow-IC, Flow-CS Human, Mouse
Helminen O, Huhta H, Takala H et al. Increased Toll-like receptor 5 expression indicates esophageal columnar dysplasia. Virchows Arch 2013 Nov 13 [PMID: 24221343] (IHC-P, Human) IHC-P Human
Show All 28 Publications.

Reviews for TLR5 Antibody (NBP2-24787) (0)

There are no reviews for TLR5 Antibody (NBP2-24787). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TLR5 Antibody (NBP2-24787) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-24787AF405
Alexa Fluor 488 Labeled NBP2-24787AF488
Alexa Fluor 647 Labeled NBP2-24787AF647
Alexa Fluor 700 Labeled NBP2-24787AF700
Allophycocyanin Labeled NBP2-24787APC
Biotin Labeled NBP2-24787B
DyLight 350 Labeled NBP2-24787UV
DyLight 405 Labeled NBP2-24787V
DyLight 488 Labeled NBP2-24787G
DyLight 550 Labeled NBP2-24787R
DyLight 650 Labeled NBP2-24787C
DyLight 680 Labeled NBP2-24787FR
DyLight 755 Labeled NBP2-24787IR
FITC Labeled NBP2-24784
HRP Labeled NBP2-24787H
PE Labeled NBP2-24783
PerCP Labeled NBP2-24787PCP

Additional TLR5 Products

Bioinformatics Tool for TLR5 Antibody (NBP2-24787)

Discover related pathways, diseases and genes to TLR5 Antibody (NBP2-24787). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TLR5 Antibody (NBP2-24787)

Discover more about diseases related to TLR5 Antibody (NBP2-24787).
 

Pathways for TLR5 Antibody (NBP2-24787)

View related products by pathway.

PTMs for TLR5 Antibody (NBP2-24787)

Learn more about PTMs related to TLR5 Antibody (NBP2-24787).
 

Research Areas for TLR5 Antibody (NBP2-24787)

Find related products by research area.

Blogs on TLR5.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TLR5 Antibody (19D759.2) and receive a gift card or discount.

Bioinformatics

Gene Symbol TLR5
Entrez