TLR5 Antibody (19D759.2)

Images

 
Western Blot: TLR5 Antibody (19D759.2) [NBP2-24787] - BEAS-2B cells whole cell lysate. Image from verified customer review.
Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) [NBP2-24787] - Analysis of TLR5 in FFPE mouse brain tissue using TLR5 antibody at 5 ug/ml.
Biological Strategies: Flow Cytometry: TLR5 Antibody (19D759.2) [NBP2-24787] - Analysis using the PE conjugate of NBP2-24787. Staining of CD14-/CD83+/TLR5+ diffferentiated myeloid dendritic cells (mDCs). PBMCs ...read more
Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) [NBP2-24787] - Human skin stained with TLR5 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.
Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) [NBP2-24787] - Analysis of FFPE tissue section of normal human skin using 5 ug/ml concentration of TLR5 antibody (clone 19D759.2). Intense membrane staining of ...read more
Flow Cytometry: TLR5 Antibody (19D759.2) [NBP2-24787] - Intracellular flow analysis of TLR5 in human monocytes using 0.5 ug of TLR5 antibody. Shaded histogram represents cells without antibody; green represents isotype ...read more
Flow Cytometry: TLR5 Antibody (19D759.2) [NBP2-24787] - Analysis using the FITC conjugate of NBP2-24787. Staining of TLR5 in PBMCs using 0.5 ug/10^6 cells of this antibody. Shaded histogram represents cells without ...read more
Flow Cytometry: TLR5 Antibody (19D759.2) [NBP2-24787] - Analysis using the PE conjugate of NBP2-24787. Staining of TLR5 in human PBMCs using 1 ug/10^6 cells of this antibody. Shaded histogram represents cells without ...read more
TLR5 Antibody (19D759.2) [NBP2-24787] - Staining in cultured fixed non-permeabilized MCF7 breast cancer cells (ATCC). EpCAM (cell membrane) staining is represented in green (FITC filter), nuclei staining is represented ...read more

Product Details

Summary
Reactivity Hu, Mu, CaSpecies Glossary
Applications WB, DB, Flow, Flow-CS, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
19D759.2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

TLR5 Antibody (19D759.2) Summary

Immunogen
This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of human TLR5 between amino acids 700-800. The antibody also reacts with mouse TLR5.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TLR5
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Dot Blot
  • Flow Cytometry 0.5-1 ug/10^6 cells
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 10 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation
  • Flow (Cell Surface)
  • Flow (Intracellular) 0.5-1 ug/10^6 cells
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Use in Flow-cell surface and Flow-intracellular reported in scientific literature (PMID 24412598). Use in Immunohistochemistry reported in scientific literature (PMID 27392931). Use in Dot blot reported in scientific literature (PMID: 27248820). ICC/IF reported in a verified customer review.
Agonist
Reviewed Applications
Read 1 Review rated 4
using
NBP2-24787 in the following applications:

Publications
Read Publications using
NBP2-24787 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TLR5 Antibody (19D759.2)

  • MGC126430
  • MGC126431
  • SLEB1FLJ10052
  • TIL-3
  • TIL3Toll/interleukin-1 receptor-like protein 3
  • TLR5
  • toll-like receptor 5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for TLR5 Antibody (NBP2-24787)(32)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 8 applications: DB, Flow, Flow-CS, Flow-IC, ICC/IF, IHC, IHC-P, WB.


Filter By Application
DB
(1)
Flow
(3)
Flow-CS
(4)
Flow-IC
(2)
ICC/IF
(1)
IHC
(3)
IHC-P
(13)
WB
(5)
All Applications
Filter By Species
Human
(19)
Mouse
(5)
All Species
Showing Publications 1 - 10 of 32. Show All 32 Publications.
Publications using NBP2-24787 Applications Species
Ruuskanen M, Leivo I, Minn H et al. Expression of toll-like receptors in non-endemic nasopharyngeal carcinoma BMC Cancer Jun 25 2019 [PMID: 31238894] (IHC-P, Human) IHC-P Human
Haeggblom L, Nasman A, Ramqvist T et al. TLR5 and TLR7 are differentially expressed in human papillomavirus-positive and negative base of tongue squamous cell carcinoma, and TLR7 may have an independent prognostic influence Acta Otolaryngol. Feb 22 2019 [PMID: 30794027] (IHC-P, Human) IHC-P Human
Kylma AK, Jouhi L, Listyarifah D et al. Treponema denticola chymotrypsin-like protease as associated with HPV-negative oropharyngeal squamous cell carcinoma. Br. J. Cancer. Jun 22 2018 [PMID: 29930251] (IHC, Human) IHC Human
Na Bon Hyang, Hoang Thi Xoan, Kim Jae Young et al. Hsp90 Inhibition Reduces TLR5 Surface Expression and NF-kappa B Activation in Human Myeloid Leukemia THP-1 Cells. BioMed Research International 2018 Feb 14 [PMID: 29651431] (Flow, WB, Human) Flow, WB Human
Larsson O, Tengroth L, Xu Y et al. Substance P represents a novel first-line defense mechanism in the nose. J. Allergy Clin. Immunol. 2017 Feb 20 [PMID: 28219705] (Flow, Mouse) Flow Mouse
Helminen O, Huhta H, Leppanen J et al. Nuclear localization of Toll-like receptor 5 in Barrett's esophagus and esophageal adenocarcinoma is associated with metastatic behavior Virchows Arch. 2016 Jul 09 [PMID: 27392931] (IHC, Human) IHC Human
Huhta H, Helminen O, Kauppila JH et al. The Expression of Toll-like Receptors in Normal Human and Murine Gastrointestinal Organs and the Effect of Microbiome and Cancer J. Histochem. Cytochem. 2016 Aug 01 [PMID: 27370795] (IHC-P, Human) IHC-P Human
Jeon YK, Kim CK, Koh J et al. Pellino-1 confers chemoresistance in lung cancer cells by upregulating cIAP2 through Lys63-mediated polyubiquitination. Oncotarget. Jul 5 2016 [PMID: 27248820] (DB, Human) DB Human
Carevic M, Oz H, Fuchs K et al. CXCR1 Regulates Pulmonary Anti-Pseudomonas Host Defense. J Innate Immun. 2016 Mar 08 [PMID: 26950764]

Details:
This citation used the FITC version of this antibody.
Makinen LK, Atula T, Hayry V et al. Predictive role of toll-like receptors 2, 4, and 9 in oral tongue squamous cell carcinoma. Oral Oncol. 2014 Sep 25 [PMID: 25264223] (IHC-P, Human)

Details:
TLR5 antibody used at 1:200 dilution for IHC-P on oral tongue squamous cell carcinoma tissues / OTSCC (Stage I-II patients) - TMA paraffin sections, antigen retrieval with PreTreatment-module in Tris-HCl buffer pH 8.5 at 98C for 20 min, Autostainer based assay with 1 hour primary incubation time followed by detection using REAL EnVision/HRP-DAB system (Fig 1, Cytoplasmic staining pattern).
IHC-P Human
Show All 32 Publications.

Review for TLR5 Antibody (NBP2-24787) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: maccaca mulatta.

Reviews using NBP2-24787:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
maccaca mulatta
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin TLR5 NBP2-24787
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reviewed by:
Monica Sauter
IHC-P maccaca mulatta 04/06/2018
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedformalin fixed paraffin embedded macaque retina tissue
Speciesmaccaca mulatta
LotCJYR01

Comments

Commentsheat mediated antigen retrieval (pH 6 citrate buffer)
1:50 dilution of antibody, overnight at 4C
goat anti-mouse AlexaFluor 488 secondary antibody (1:400, 1 hr RT)

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TLR5 Antibody (NBP2-24787) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-24787AF350
Alexa Fluor 405 NBP2-24787AF405
Alexa Fluor 488 NBP2-24787AF488
Alexa Fluor 532 NBP2-24787AF532
Alexa Fluor 594 NBP2-24787AF594
Alexa Fluor 647 NBP2-24787AF647
Alexa Fluor 700 NBP2-24787AF700
Alexa Fluor 750 NBP2-24787AF750
Allophycocyanin NBP2-24787APC
Allophycocyanin/Cy7 NBP2-24787APCCY7
Biotin NBP2-24787B
DyLight 350 NBP2-24787UV
DyLight 405 NBP2-24787V
DyLight 488 NBP2-24787G
DyLight 550 NBP2-24787R
DyLight 594 NBP2-24787DL594
DyLight 650 NBP2-24787C
DyLight 680 NBP2-24787FR
DyLight 755 NBP2-24787IR
FITC NBP2-24787F
HRP NBP2-24787H
Janelia Fluor 549 NBP2-24787JF549
Janelia Fluor 646 NBP2-24787JF646
PE NBP2-24783
PE/Atto594 NBP2-24787PEATT594
PE/Cy5.5 NBP2-24787PECY55
PE/Cy7 NBP2-24787PECY7
PerCP NBP2-24787PCP

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Blogs on TLR5.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

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Recent Reviews

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5
0
4
1
3
0
2
0
1
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Monica Sauter
04/06/2018
Application: IHC-P
Species: maccaca mulatta

Bioinformatics

Gene Symbol TLR5