TLR5 Antibody (19D759.2) - BSA Free

Images

 
Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) [NBP2-24787] - Analysis of FFPE tissue section of normal human skin using 5 ug/ml concentration of TLR5 antibody (clone 19D759.2). Intense membrane staining of ...read more
Biological Strategies: Flow Cytometry: TLR5 Antibody (19D759.2) [NBP2-24787] - Analysis using the PE conjugate of NBP2-24787. Staining of CD14-/CD83+/TLR5+ diffferentiated myeloid dendritic cells (mDCs). PBMCs ...read more
Western Blot: TLR5 Antibody (19D759.2) [NBP2-24787] - BEAS-2B cells whole cell lysate. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: TLR5 Antibody (19D759.2) [NBP2-24787] - Staining using in-vitro cultured fixed non-permeabilized MCF7 breast cancer cells (ATCC). EpCAM (cell membrane) staining is represented in ...read more
Immunohistochemistry: TLR5 Antibody (19D759.2) [NBP2-24787] - TLR5 and TLR7 expression in nasopharyngeal carcinoma and in the normal epithelium. Mild positive TLR5 expression in NK-U. Strong positive TLR5 expression in ...read more
Flow Cytometry: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787] - Flow analysis of TLR5 in mouse MC38 cells. Image from verified customer review.
Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) [NBP2-24787] - Human skin stained with TLR5 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.
Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) [NBP2-24787] - Analysis of TLR5 in FFPE mouse brain tissue using TLR5 antibody at 5 ug/ml.
Flow Cytometry: TLR5 Antibody (19D759.2) [NBP2-24787] - Intracellular flow analysis of TLR5 in human monocytes using 0.5 ug of TLR5 antibody. Shaded histogram represents cells without antibody; green represents isotype ...read more
TLR5 and TLR7 expression in nasopharyngeal carcinoma and in the normal epithelium. a H&E staining in non-keratinizing undifferentiated carcinoma (NK-U). b H&E staining in keratinizing squamous cell carcinoma (KSCC). c ...read more

Product Details

Summary
Reactivity Hu, Mu, CaSpecies Glossary
Applications WB, DB, Flow, ICC/IF, IHC, IP
Clone
19D759.2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

TLR5 Antibody (19D759.2) - BSA Free Summary

Immunogen
This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of human TLR5 between amino acids 700-800. The antibody also reacts with mouse TLR5.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TLR5
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dot Blot reported in scientific literature (PMID 27248820)
  • Flow (Cell Surface) reported in scientific literature (PMID 24412598)
  • Flow (Intracellular) 0.5-1 ug/10^6 cells. Use reported in scientific literature (PMID 24412598)
  • Flow Cytometry 0.5-1 ug/10^6 cells
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml. Use reported by customer review
  • Immunohistochemistry 1:10-1:500. Use reported in scientific literature (PMID 27392931)
  • Immunohistochemistry-Frozen 10 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation
  • Western Blot 1-3 ug/ml
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Agonist
Reviewed Applications
Read 1 Review rated 4
using
NBP2-24787 in the following applications:

Publications
Read Publications using
NBP2-24787 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TLR5 Antibody (19D759.2) - BSA Free

  • MGC126430
  • MGC126431
  • SLEB1FLJ10052
  • TIL-3
  • TIL3Toll/interleukin-1 receptor-like protein 3
  • TLR5
  • toll-like receptor 5

Background

The Toll like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin 1 receptor-associated kinase (IRAK) signaling pathway. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56566
Species: Bv, Hu, Ma, Mu, Po, Rt
Applications: B/N, ChIP, CyTOF-ready, DB, Dual ISH-IHC, ELISA(Cap), ELISA, Flow-CS, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, KD, KO, PAGE, WB
NB100-56722
Species: Ca, Hu, Mu, Rb, Rt
Applications: B/N, DB, ELISA, Flow-CS, Flow, Func, ICC/IF, IP, In vitro, WB
NBP2-24875
Species: Ca, Hu, Mu
Applications: BA, B/N, Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP2-24729
Species: Ca, Eq, Hu, Pm, Mu, Pm, Rt
Applications: B/N, CyTOF-ready, DB, ELISA, Flow-IC, Flow, Func, ICC/IF, IHC, IHC-P, IP, In vitro, KD, Simple Western, WB
M6000B
Species: Mu
Applications: ELISA
NBP2-24906
Species: Hu, Mu, Rt
Applications: BA, DB, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, WB
NB100-56563
Species: Hu, Mu, Rt
Applications: DB, Flow-CS, Flow-IC, Flow, IHC, IHC-P, WB
MAB208
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
NB100-56583
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
NBP2-29328
Species: Bv, Hu, Mu, Rt, Xp, Ye, Ze
Applications: BindInhib, B/N, ELISA, Flow, Func-Inh, IHC, In vitro, In vivo
NB100-56536
Species: Hu
Applications: Flow-CS, Flow-IC, Flow, IHC, IHC-P, WB
NBP2-24917
Species: Hu, Mu, Rb
Applications: CyTOF-ready, DB, Flow-CS, Flow-IC, Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
DY417
Species: Mu
Applications: ELISA
MAB1230
Species: Hu, Mu, Rt
Applications: ICC, IHC, KO, Simple Western, WB
DDX0490P-100
Species: Hu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-93743
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
DC140
Species: Hu
Applications: ELISA
DCP00
Species: Hu
Applications: ELISA
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
Supplier Logo

Publications for TLR5 Antibody (NBP2-24787)(40)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 8 applications: Cytometric Bead Assay Standard, FLOW, Flow Cytometry Control, Flow-CS, ICC/IF, IF/IHC, IHC-P, WB.


Filter By Application
Cytometric Bead Assay Standard
(1)
FLOW
(3)
Flow Cytometry Control
(2)
Flow-CS
(4)
ICC/IF
(1)
IF/IHC
(4)
IHC-P
(13)
WB
(6)
All Applications
Filter By Species
Human
(21)
Mouse
(5)
All Species
Showing Publications 1 - 10 of 40. Show All 40 Publications.
Publications using NBP2-24787 Applications Species
Kitaura A, Nishinaka T, Hamasaki S, et al. Advanced glycation end-products reduce lipopolysaccharide uptake by macrophages PloS one 2021-01-25 [PMID: 33493233]
Kylma AK, Jouhi L, Listyarifah D et al. Treponema denticola chymotrypsin-like protease as associated with HPV-negative oropharyngeal squamous cell carcinoma. Br. J. Cancer. 2018-06-22 [PMID: 29930251] (IF/IHC, Human) IF/IHC Human
Sipola S. COLECTOMY IN AN ICU PATIENT POPULATION. Thesis 2014-01-01 (IHC-P, Human)

Details:
Normal and diseased colon, The TLR5 mAb was used at 1:50.
IHC-P Human
Eskuri M, Kemi N, Helminen O et al. Toll-like receptors 1, 2, 4, 5, and 6 in gastric cancer Virchows Archiv : an international journal of pathology 2023-09-26 [PMID: 37750927]
Beilmann-Lehtonen I, HagstrOm J, Mustonen H et al. High Tissue TLR5 Expression Predicts Better Outcomes in Colorectal Cancer Patients Oncology 2021-06-17 [PMID: 34139707]
Kairaluoma V, Kemi N, Huhta H et al. Toll-like receptor 5 and 8 in hepatocellular carcinoma APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2021-05-05 [PMID: 33950532]
Shang L, Deng D, Roffel S, Gibbs S Differential influence of Streptococcus mitis on host response to metals in reconstructed human skin and oral mucosa Contact Derm. 2020-07-16 [PMID: 32677222] (WB, Human) WB Human
KylmA AK, Tolvanen TA, CarpEn T et al. Elevated TLR5 expression in vivo and loss of NF-kappa B activation via TLR5 in vitro detected in HPV-negative oropharyngeal squamous cell carcinoma Exp. Mol. Pathol. 2020-03-30 [PMID: 32240617] (IF/IHC, Human) IF/IHC Human
Kasurinen A, Hagstrom J, Laitinen A et al. Evaluation of toll-like receptors as prognostic biomarkers in gastric cancer: high tissue TLR5 predicts a better outcome Sci Rep [PMID: 31467388] (IHC-P, Human) IHC-P Human
Ruuskanen M, Leivo I, Minn H et al. Expression of toll-like receptors in non-endemic nasopharyngeal carcinoma BMC Cancer 2019-06-25 [PMID: 31238894] (IHC-P, Human) IHC-P Human
Show All 40 Publications.

Review for TLR5 Antibody (NBP2-24787) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: maccaca mulatta.

Reviews using NBP2-24787:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
maccaca mulatta
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin TLR5 NBP2-24787
Enlarge
4
reviewed by:
Monica Sauter
IHC-P maccaca mulatta 04/06/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedformalin fixed paraffin embedded macaque retina tissue
Speciesmaccaca mulatta
LotCJYR01

Comments

Commentsheat mediated antigen retrieval (pH 6 citrate buffer)
1:50 dilution of antibody, overnight at 4C
goat anti-mouse AlexaFluor 488 secondary antibody (1:400, 1 hr RT)

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TLR5 Antibody (NBP2-24787) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional TLR5 Products

Diseases for TLR5 Antibody (NBP2-24787)

Discover more about diseases related to TLR5 Antibody (NBP2-24787).
 

Pathways for TLR5 Antibody (NBP2-24787)

View related products by pathway.

PTMs for TLR5 Antibody (NBP2-24787)

Learn more about PTMs related to TLR5 Antibody (NBP2-24787).
 

Research Areas for TLR5 Antibody (NBP2-24787)

Find related products by research area.

Blogs on TLR5.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria ...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Monica Sauter
04/06/2018
Application: IHC-P
Species: maccaca mulatta

Bioinformatics

Gene Symbol TLR5