TLR9 Antibody (26C593.2)


Simple Western: TLR9 Antibody (26C593.2) [NBP2-24729] - Lane view shows a specific band for TLR9 in 0.5 mg/ml of Ramos lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation more
Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR9 protein on epithelial cells. HNEC, Detroit-562 and FaDu were stained intracellularly with PE-Ab against TLR9 (open histograms) or appropriate more
Genetic Strategies: Knockdown Validated: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR-9 protein in HB cells before and after 48 hr of transfection. Beta-actin was used as an internal control. Image more
Immunocytochemistry/ Immunofluorescence: TLR9 Antibody (26C593.2) [NBP2-24729] - Cbl-b is required for the endocytic transit of TLR9. Representative confocal microscopic images of splenocytes from mice with indicated more
Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Analysis of colonic LP or peritoneal cavity (PerC) of normal mice were evaluated by flow cytometry. B cells were intracellularly stained with the anti-TLR9 more
Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Gating strategy for the detection of TLR-7 and -9 in B cell subsets. PBMC were isolated from whole blood and stained for surface markers before cells were fixed, more
Immunohistochemistry-Paraffin: TLR9 Antibody (26C593.2) [NBP2-24729] - Monkey retina tissue. Image from verified customer review.
Western Blot: TLR9 Antibody (26C593.2) [NBP2-24729] - Analysis of TLR9 in A) human PBMC, B) human intestine, C) mouse intestine, and D) rat intestine tissue lysates using this antibody at a dilution of 3 ug/ml.
Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR9 on turbinate epithelial cells from healthy controls compared to turbinate and polyp epithelial cells from patients with CRSwNP, n = 5 (A). more

Product Details

Reactivity Hu, Mu, Rt, Ca, Eq, Pm, PmSpecies Glossary
Applications WB, Simple Western, DB, ELISA, Flow, Flow-IC, Func, ICC/IF, IHC, IHC-P, In vitro, IP, B/N, CyTOF-ready, KD
1 mg/ml
Validated by:

Genetic Strategies


Order Details

TLR9 Antibody (26C593.2) Summary

This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-300 of human TLR9 isoform A (Genbank accession no. AAF78037).
IgG1 Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 2-5 ug/ml
  • Simple Western 30 ug/ml
  • Dot Blot
  • ELISA 1:100 - 1:2000
  • Flow Cytometry
  • Functional
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:10 - 1:500
  • In vitro assay
  • Block/Neutralize
  • CyTOF-ready
  • Flow (Intracellular) 1:10 - 1:1000
  • Knockdown Validated
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. In human PBMC, a ~120 kDa band is observed. A smaller isoform, TLR9 isoform B (Genbank accession no. AAF72190) containing 975 amino acids may also be observed in some cases. Use in Functional and in vitro assays reported in scientific literature (PMID 25411258). Use in ELISA reported in multiple publications. Use in Immunoprecipitation reported in scientific literature (PMID 25871979). Use in Flow-intracellular reported in scientific literature (PMID 24986635). Use in functional reported in scientific literature (PMID: 25411258). Use in Dot blot reported in scientific literature (PMID: 27248820). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in Immunohistochemistry reported in scientific literature (PMID 27744078). Use in blocking/ neutralizing reported in scientific literature (PMID: 25338738). Knockdown validation (PMID: 31655343).
Reviewed Applications
Read 4 Reviews rated 3.8
NBP2-24729 in the following applications:

Read Publications using
NBP2-24729 in the following applications:

Reactivity Notes

Rhesus Monkey.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1 mg/ml
Protein G purified

Alternate Names for TLR9 Antibody (26C593.2)

  • CD289 antigen
  • CD289
  • TLR9
  • toll-like receptor 9


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Po, Bv, Ma
Applications: WB, ChIP, DB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, PAGE, B/N, CyTOF-ready, Dual ISH-IHC, ELISA(Cap), Flow-CS, Flow-IC, KD, KO
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, DB, ELISA, Flow, Func, ICC/IF, IP, In vitro, B/N, Flow-CS
Species: Hu, Mu, Rt
Applications: WB, DB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, BA, Flow-IC
Species: Hu, Mu, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, BA, B/N, Flow-IC
Species: Hu, Mu, Rt, Bv, Xp, Ye, Ze
Applications: ELISA
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rb
Applications: WB, Simple Western, DB, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, Flow-CS, Flow-IC
Species: Hu, Mu, Ca
Applications: WB, DB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-CS, Flow-IC
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
Species: Hu, Mu, Rt
Applications: WB, DB, Flow, IHC, IHC-P, Flow-CS, Flow-IC
Species: Hu, Mu, Rt, Po, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Dual ISH-IHC
Species: Hu
Applications: Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
Species: Hu
Applications: WB, Flow, IHC, IHC-P, Flow-CS, Flow-IC
Species: Hu, Mu, Rt
Applications: WB, Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, KO
Supplier Logo

Publications for TLR9 Antibody (NBP2-24729)(144)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 14 applications: B/N, DB, ELISA, FLOW, Flow, Flow-IC, Func, ICC/IF, IHC, IHC-P, IP, In vitro, KD, WB.

Filter By Application
In vitro
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 144. Show All 144 Publications.
Publications using NBP2-24729 Applications Species
Kuramoto K, Kim YJ, Hong JH, He C The autophagy protein Becn1 improves insulin sensitivity by promoting adiponectin secretion via exocyst binding Cell reports May 25 2021 [PMID: 34038729]
Li FJ, Surolia R, Li H et al. Citrullinated vimentin mediates development and progression of lung fibrosis Science translational medicine Mar 17 2021 [PMID: 33731433]
Niemczyk G, Fus L, Czarzasta K et al. Expression of Toll-Like Receptors in the Animal Model of Bladder Outlet Obstruction BioMed research international Dec 12 2020 [PMID: 33381567] (IHC, Rat) IHC Rat
KorimovA A, DubovY P N-Formylated Peptide Induces Increased Expression of Both Formyl Peptide Receptor 2 (Fpr2) and Toll-Like Receptor 9 (TLR9) in Schwannoma Cells-An In Vitro Model for Early Inflammatory Profiling of Schwann Cells Cells Dec 11 2020 [PMID: 33322305] (WB, Rat) WB Rat
Wang C, Chen C, Lin M et al. TLR9 Binding to Beclin 1 and Mitochondrial SIRT3 by a Sodium-Glucose Co-Transporter 2 Inhibitor Protects the Heart from Doxorubicin Toxicity Biology Oct 29 2020 [PMID: 33138323]
Richard Albert J, Au Yeung WK, Toriyama K et al. Maternal DNMT3A-dependent de novo methylation of the paternal genome inhibits gene expression in the early embryo Nat Commun Oct 27 2020 [PMID: 33110091]
Natarajan S, Ranganathan M Toll-like receptor (TLR) gene expression and immunostimulatory effect of CpG Oligonucleotides in hormone receptor positive cell line T47D and Triple negative breast cancer cell line MDA-MB-468 Immunopharmacol Immunotoxicol Jul 19 2020 [PMID: 32686546]
Zhou B, Yan J, Guo L et al. Hepatoma cell-intrinsic TLR9 activation induces immune escape through PD-L1 upregulation in hepatocellular carcinoma Theranostics May 17 2020 [PMID: 32483468] (WB, IHC, Flow, KD, Human) WB, IHC, Flow, KD Human
Du XK, Ge WY, Jing R, Pan LH Necroptosis in pulmonary macrophages mediates lipopolysaccharide-induced lung inflammatory injury by activating ZBP-1 Int. Immunopharmacol. 2019 Oct 23 [PMID: 31655343] (WB, KD, Mouse) WB, KD Mouse
Kalantari E, Abolhasani M, Roudi R et al. Co-expression of TLR-9 and MMP-13 is associated with the degree of tumour differentiation in prostate cancer Int J Exp Pathol Apr 1 2019 [PMID: 31090157] (Human) Human
Show All 144 Publications.

Reviews for TLR9 Antibody (NBP2-24729) (4) 3.84

Average Rating: 3.8
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, macaca mulatta.

Reviews using NBP2-24729:
Filter by Applications
All Applications
Filter by Species
macaca mulatta
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin TLR9 NBP2-24729
reviewed by:
Monica Sauter
IHC-P macaca mulatta 04/12/2018


Sample Testedparaffin embedded macaque retina tissue
Speciesmacaca mulatta


CommentsHeat mediated antigen retrieval (pH 6 citrate buffer)
1:25 dilution of primary antibody ON at 4C
1:400 dilution of goat anti-mouse Alexa Fluor 488
Western Blot TLR9 NBP2-24729
reviewed by:
Verified Customer
WB Human 10/20/2017


ApplicationWestern Blot
Sample Testedhuman lymphocytes, whole cell lysate


Comments3ug/mL in Milk-TBS/T
30ug of protein, 4-12% Bis-Tris gel
10 minute exposure, several bands. 250, 120, 75, 55kDA
reviewed by:
Verified Customer
WB Human 01/14/2015


ApplicationWestern Blot
Sample TestedWhole cell lysate


Blocking DetailsBSA Blocked for 1hr

Primary Anitbody

Dilution Ratio1:2500


Detection NotesChemiluminescence ChemiDoc™ XRS+ system, TLR9+ lysate control and TLR9- Lysate Control
Western Blot TLR9 NBP2-24729
reviewed by:
Mariann Kremlitzka
WB Human 11/25/2014


ApplicationWestern Blot
Sample TestedPrimary blood-derived B cells


Blocking Details5% milk powder

Primary Anitbody

Dilution Ratio1:3000

Secondary Antibody

Secondary DescriptionGoat anti-rabbit,HRP (DAKO)
Secondary Manufacturer Cat#P0448
Secondary Concentration0,25g/L


Detection NotesPositive control: total B cell lysate, Detection: ECL method (Millipore), 1 minute exposure

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TLR9 Antibody (NBP2-24729). (Showing 1 - 1 of 1 FAQs).

  1. I am a bit unsure about your antibody NBP2-24729 specific against TLR9. I used it for  probing mouse B cell lysate on Proteisimple WES. On their website the recommend your antibody and claim that it detects a specific signal at 90 kDa. But on your website simple western detects a band at 200 kDa?
    • This came during the race to 1000 and we had many people generating and reviewing data.  In retrospect, this was one that probably should not have made it out. As I reviewed the run data, it looks as though the MW ladder did not assign correctly.  When this was corrected, the band at 200 actually shifts higher, up to 280 kDa.  This band now overlaps with the 280 kDa fluorescent standard which is prone to have non-specific interactions with some antibodies.  Those two things make this non-supportive in Simple Western.   We ran Raji lysate on the same run.  This antibody detects a very broad band with a peak at ~150-165 kDa, closer to expected MW.  However, the baseline for this samples was also very high.  If we were presented with this data set today, it would not be considered supportive.  The other information on the Protein Simple web site likely came from a customer. 

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-24729AF350
Alexa Fluor 405 NBP2-24729AF405
Alexa Fluor 488 NBP2-24729AF488
Alexa Fluor 532 NBP2-24729AF532
Alexa Fluor 594 NBP2-24729AF594
Alexa Fluor 647 NBP2-24729AF647
Alexa Fluor 700 NBP2-24729AF700
Alexa Fluor 750 NBP2-24729AF750
Allophycocyanin NBP2-24729APC
Biotin NBP2-24890
DyLight 350 NBP2-24729UV
DyLight 405 NBP2-24729V
DyLight 488 NBP2-24729G
DyLight 550 NBP2-24729R
DyLight 594 NBP2-24729DL594
DyLight 650 NBP2-24729C
DyLight 680 NBP2-24729FR
DyLight 755 NBP2-24729IR
FITC NBP2-24729F
HRP NBP2-24729H
Janelia Fluor 549 NBP2-24729JF549
Janelia Fluor 646 NBP2-24729JF646
PE NBP2-24907
PerCP NBP2-24729PCP

Additional TLR9 Products

Bioinformatics Tool for TLR9 Antibody (NBP2-24729)

Discover related pathways, diseases and genes to TLR9 Antibody (NBP2-24729). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TLR9 Antibody (NBP2-24729)

Discover more about diseases related to TLR9 Antibody (NBP2-24729).

Pathways for TLR9 Antibody (NBP2-24729)

View related products by pathway.

PTMs for TLR9 Antibody (NBP2-24729)

Learn more about PTMs related to TLR9 Antibody (NBP2-24729).

Research Areas for TLR9 Antibody (NBP2-24729)

Find related products by research area.

Blogs on TLR9.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

Topics in CD11b: The innate immune response
Integrins are transmembrane receptors composed of alpha and beta chains, where beta-integrins are mainly expressed in leukocytes. Leukocytes are white blood cells that act in the immune system to defend our body against foreign pathogens.  Integrin...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

TLR9: For Whom the Cell Tolls
The Toll-like receptor 9 (TLR9) protein, also known as CD289, belongs to the family of Toll-like receptor (TLR) proteins which play a large role in pathogen recognition and the activation of innate immunity. Scientists using TLR9 antibodies have found...  Read full blog post.

TLR9: Tollgate to Immunity
Toll-like receptors (TLRs) play an essential role in the activation of innate immunity, and TLRs are expressed in a large number of immune cells as well as in epithelial cells. TLR9 recognizes synthetic oligodeoxynucleotides (ODN) containing unmethyla...  Read full blog post.

TLR9, Infectious Disease and Cancer
Toll-like receptor 9 (TLR9) is a protein encoded by TLR9 gene in humans. It is also known as cluster of differentiation 289 (CD289) and is a member of TLR family. Proteins from TLR family are transmembrane proteins that expressed in both antigen-resen...  Read full blog post.

TLR9 Antibodies in Immunity Research
Toll-like receptor 9 (TLR9) is a member of the toll-like receptor family that plays a key role in pathogen recognition and activation of innate immunity. Scientists using TLR9 antibodies have found the protein is highly conserved from Drosophila to hu...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Recent Reviews


Monica Sauter
Application: IHC-P
Species: macaca mulatta

Verified Customer
Application: WB
Species: Human

Verified Customer
Application: WB
Species: Human


Gene Symbol TLR9