Simple Western: TLR9 Antibody (26C593.2) [NBP2-24729] - Lane view shows a specific band for TLR9 in 0.5 mg/ml of Ramos lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation ...read more
Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR9 protein on epithelial cells. HNEC, Detroit-562 and FaDu were stained intracellularly with PE-Ab against TLR9 (open histograms) or appropriate ...read more
Genetic Strategies: Knockdown Validated: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR-9 protein in HB cells before and after 48 hr of transfection. Beta-actin was used as an internal control. Image ...read more
Immunocytochemistry/ Immunofluorescence: TLR9 Antibody (26C593.2) [NBP2-24729] - Cbl-b is required for the endocytic transit of TLR9. Representative confocal microscopic images of splenocytes from mice with indicated ...read more
Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Analysis of colonic LP or peritoneal cavity (PerC) of normal mice were evaluated by flow cytometry. B cells were intracellularly stained with the anti-TLR9 ...read more
Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Gating strategy for the detection of TLR-7 and -9 in B cell subsets. PBMC were isolated from whole blood and stained for surface markers before cells were fixed, ...read more
Western Blot: TLR9 Antibody (26C593.2) [NBP2-24729] - Analysis of TLR9 in A) human PBMC, B) human intestine, C) mouse intestine, and D) rat intestine tissue lysates using this antibody at a dilution of 3 ug/ml.
Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR9 on turbinate epithelial cells from healthy controls compared to turbinate and polyp epithelial cells from patients with CRSwNP, n = 5 (A). ...read more
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. In human PBMC, a ~120 kDa band is observed. A smaller isoform, TLR9 isoform B (Genbank accession no. AAF72190) containing 975 amino acids may also be observed in some cases. Use in Functional and in vitro assays reported in scientific literature (PMID 25411258). Use in ELISA reported in multiple publications. Use in Immunoprecipitation reported in scientific literature (PMID 25871979). Use in Flow-intracellular reported in scientific literature (PMID 24986635). Use in functional reported in scientific literature (PMID: 25411258). Use in Dot blot reported in scientific literature (PMID: 27248820). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in Immunohistochemistry reported in scientific literature (PMID 27744078). Use in blocking/ neutralizing reported in scientific literature (PMID: 25338738). Knockdown validation (PMID: 31655343).
Bauer JMJ. Interferon-b moduliert die Expression von Toll-like Rezeptoren in mononuklearen Blutzellen und induziert Toll-like Rezeptor 7 in plasmazytoiden dendritischen Zellen von Patienten mit Multipler Sklerose. thesis. 2015 Nov 12
Sasaki K, Tanaka A, Nagatomo H, Ogawa H. Characterization of Toll-Like Receptor 9 expression during Mouse Preimplantation Development. J Genit Syst Disor 5: 1. J Genit Syst Disor. Jan 15 2016 (WB, ICC/IF, Mouse)
FAQs for TLR9 Antibody (NBP2-24729). (Showing 1 - 1 of 1 FAQs).
I am a bit unsure about your antibody NBP2-24729 specific against TLR9. I used it for probing mouse B cell lysate on Proteisimple WES. On their website the recommend your antibody and claim that it detects a specific signal at 90 kDa. But on your website simple western detects a band at 200 kDa?
This came during the race to 1000 and we had many people generating and reviewing data. In retrospect, this was one that probably should not have made it out. As I reviewed the run data, it looks as though the MW ladder did not assign correctly. When this was corrected, the band at 200 actually shifts higher, up to 280 kDa. This band now overlaps with the 280 kDa fluorescent standard which is prone to have non-specific interactions with some antibodies. Those two things make this non-supportive in Simple Western. We ran Raji lysate on the same run. This antibody detects a very broad band with a peak at ~150-165 kDa, closer to expected MW. However, the baseline for this samples was also very high. If we were presented with this data set today, it would not be considered supportive. The other information on the Protein Simple web site likely came from a customer.
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