TLR9 Antibody (26C593.2)

Images

 
Western Blot: TLR9 Antibody (26C593.2) [NBP2-24729] - Analysis of TLR9 in A) human PBMC, B) human intestine, C) mouse intestine, and D) rat intestine tissue lysates using Azide free TLR9 antibody at a dilution of 3 ...read more
Immunohistochemistry-Paraffin: TLR9 Antibody (26C593.2) [NBP2-24729] - Analysis using the Azide Free version of NBP2-24729. Staining of human spleen probed with TLR9 antibody at 5 ug/ml. Human tissue was used for this ...read more
Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Flow analysis of TLR9 in human PBMC using 0.5 ug of TLR9 antibody (red) and isotype control antibody (green).
Western Blot: TLR9 Antibody (26C593.2) [NBP2-24729] - analysis of TLR9 in human B cells using anti-TLR9 antibody. Image from verified customer review.
Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Intracellular flow cytometric analysis of TLR9 FITC in human B cells using 1ug/10^6 cells of NBP2-24908. Cells were primarily stained with PE-conjugated CD19 ...read more
Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Intracellular analysis of TLR9 FITC in Ramos cells using 0.5 ug of this antibody. Green represents isotype control; red represents anti-TLR9 antibody. TLR ...read more
Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Analysis using the Azide Free version of NBP2-24729. Staining of TLR9 in Ramos cells using 0.5 ug of this antibody. Shaded histogram represents Ramos cells without ...read more
Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Analysis using the FITC conjugate of NBP2-24729. Staining of TLR9 in Ramos cells using 0.5 ug of this antibody. Green represents isotype control ; red represents ...read more
Flow (Intracellular): TLR9 Antibody (26C593.2) [NBP2-24729] - Analysis using the PE conjugate of NBP2-24729. Staining of TLR9 in human PBMCs using 0.2 ug of this antibody. Shaded histogram represents cells without ...read more
Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Analysis using the PE conjugate of NBP2-24729. Staining of TLR9 in Ramos cells using 0.1 ug of antibody. Shaded histogram represents Ramos cells without antibody; ...read more
Simple Western: TLR9 Antibody (26C593.2) [NBP2-24729] - Simple Western lane view shows a specific band for TLR9 in 0.5 mg/ml of Ramos lysate. This experiment was performed under reducing conditions using the 66-440 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Eq, PmSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, Flow-IC, Func, ICC/IF, IHC, IHC-P, In vitro, IP, CyTOF-ready
Clone
26C593.2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

TLR9 Antibody (26C593.2) Summary

Immunogen
This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-300 of human TLR9 isoform A (Genbank accession no. AAF78037).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TLR9
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-5 ug/ml
  • Simple Western 30 ug/ml
  • ELISA 1:100 - 1:2000
  • Flow Cytometry
  • Functional
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:10 - 1:500
  • In vitro assay
  • CyTOF-ready
  • Flow (Intracellular) 1:10 - 1:1000
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. In human PBMC, a ~120 kDa band is observed. A smaller isoform, TLR9 isoform B (Genbank accession no. AAF72190) containing 975 amino acids may also be observed in some cases. Use in Functional and in vitro reported in scientific literature (PMID 25411258). Use in ELISA reported in multiple pieces of scientific literature Use in Immunoprecipitation reported in scientific literature (PMID 25871979). Use in Flow-intracellular reported in scientific literature (PMID 24986635). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in Immunohistochemistry reported in scientific literature (PMID 27744078)
Agonist
Reviewed Applications
Read 3 Reviews rated 3.7
using
NBP2-24729 in the following applications:

Publications
Read Publications using
NBP2-24729 in the following applications:

Reactivity Notes

Rhesus Monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for TLR9 Antibody (26C593.2)

  • CD289 antigen
  • CD289
  • TLR9
  • toll-like receptor 9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TLR9 Antibody (NBP2-24729)(132)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 13 applications: B/N, DB, ELISA, FLOW, Flow, Flow-IC, Func, ICC/IF, IHC, IHC-P, IP, In vitro, WB.


Filter By Application
B/N
(1)
DB
(1)
ELISA
(2)
FLOW
(13)
Flow
(7)
Flow-IC
(2)
Func
(1)
ICC/IF
(11)
IHC
(4)
IHC-P
(10)
IP
(1)
In vitro
(2)
WB
(10)
All Applications
Filter By Species
Human
(37)
Mouse
(9)
Rat
(1)
Canine
(1)
All Species
Showing Publications 1 - 10 of 132. Show All 132 Publications.
Publications using NBP2-24729 Applications Species
Leppanen J, Helminen O, Huhta H et al. High toll-like receptor (TLR) 9 expression is associated with better prognosis in surgically treated pancreatic cancer patients. Virchows Arch. Feb 12 2017 [PMID: 28191612] (IHC, Human) IHC Human
Huggins J, Pellegrin T, Felgar RE et al. CpG DNA activation and plasma-cell differentiation of CD27- naive human B cells Blood (FLOW)

Details:
This publication used the FITC conjugated form of this antibody (Cat# NBP2-24908).
FLOW
Helminen O, Huhta H, Kauppila JH et al. Localization of nucleic acid-sensing toll-like receptors in human and mouse pancreas. APMIS. Dec 28 2016 [PMID: 28028829] (IHC, Human) IHC Human
Lim JS, Kim HS, Nguyen KC, Cho KA. The role of TLR9 in stress-dependent autophagy formation. Biochem Biophys Res Commun 2016 Dec 09 [PMID: 27793667] (Human) Human
Mu L, Wang Y, Wang Y et al. Tumor Location and Survival Outcomes in Adult Patients with Supratentorial Glioblastoma by Levels of Toll-like Receptor 9 Expression. World Neurosurg. Oct 12 2016 [PMID: 27744078] (IHC, Human) IHC Human
Banus JFB. Understanding the role of Toll-like receptors in the lower gastrointestinal tract. Thesis. 2014 (IHC-P, Mouse)

Details:
Citation using the Biotin form of this antibody.
IHC-P Mouse
Chandler MR, Keene KS, Tuomela JM, Forero-Torres A et al. Lower frequency of TLR9 variant associated with protection from breast cancer among African Americans PLoS ONE 2017 Sep 08 [PMID: 28886076] (Human) Human
Heftrig D, Sturm R, Oppermann E et al. Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients. Mediators Inflamm. [PMID: 28255201] (Flow, Human)

Details:
Citation using the Alexa Fluor 647 form of this antibody.
Flow Human
Larsson O, Tengroth L, Xu Y et al. Substance P represents a novel first-line defense mechanism in the nose. J Allergy Clin Immunol. [PMID: 28219705] (Flow, ICC/IF, IHC, Mouse)

Details:
Citation using the Alexa Fluor 647 form of this antibody.
Flow, ICC/IF, IHC Mouse
Sipola S. Colectomy In An Icu Patient Population Thesis 2014 (IHC-P, Human)

Details:
This reference used the Azide Free version of NBP2-24729.
IHC-P Human
Show All 132 Publications.

Reviews for TLR9 Antibody (NBP2-24729) (3) 3.73

Average Rating: 3.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NBP2-24729:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Western Blot TLR9 NBP2-24729
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reviewed by:
Anonymous
WB Human 10/20/2017
View

Summary

ApplicationWestern Blot
Sample Testedhuman lymphocytes, whole cell lysate
SpeciesHuman
LotA-1

Comments

Comments3ug/mL in Milk-TBS/T
30ug of protein, 4-12% Bis-Tris gel
10 minute exposure, several bands. 250, 120, 75, 55kDA
 
reviewed by:
Anonymous
WB Human 01/14/2015
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate
SpeciesHuman
Lot1005

Blocking

Blocking DetailsBSA Blocked for 1hr

Primary Anitbody

Dilution Ratio1:2500

Details

Detection NotesChemiluminescence ChemiDoc™ XRS+ system, TLR9+ lysate control and TLR9- Lysate Control
Western Blot TLR9 NBP2-24729
Enlarge
reviewed by:
Mariann Kremlitzka
WB Human 11/25/2014
View

Summary

ApplicationWestern Blot
Sample TestedPrimary blood-derived B cells
SpeciesHuman
Lot#1005

Blocking

Blocking Details5% milk powder

Primary Anitbody

Dilution Ratio1:3000

Secondary Antibody

Secondary DescriptionGoat anti-rabbit,HRP (DAKO)
Secondary Manufacturer Cat#P0448
Secondary Concentration0,25g/L

Details

Detection NotesPositive control: total B cell lysate, Detection: ECL method (Millipore), 1 minute exposure

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TLR9 Antibody (NBP2-24729) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-24729AF405
Alexa Fluor 488 Labeled NBP2-24729AF488
Alexa Fluor 647 Labeled NBP2-24729AF647
Alexa Fluor 700 Labeled NBP2-24729AF700
Allophycocyanin Labeled NBP2-24729APC
Biotin Labeled NBP2-24890
DyLight 350 Labeled NBP2-24729UV
DyLight 405 Labeled NBP2-24729V
DyLight 488 Labeled NBP2-24729G
DyLight 550 Labeled NBP2-24729R
DyLight 650 Labeled NBP2-24729C
DyLight 680 Labeled NBP2-24729FR
DyLight 755 Labeled NBP2-24729IR
HRP Labeled NBP2-24729H
PE Labeled NBP2-24907
PerCP Labeled NBP2-24729PCP

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Bioinformatics Tool for TLR9 Antibody (NBP2-24729)

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Blogs on TLR9.

Topics in CD11b: The innate immune response
Integrins are transmembrane receptors composed of alpha and beta chains, where beta-integrins are mainly expressed in leukocytes. Leukocytes are white blood cells that act in the immune system to defend our body against foreign pathogens.  Integrin...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

TLR9: For Whom the Cell Tolls
The Toll-like receptor 9 (TLR9) protein, also known as CD289, belongs to the family of Toll-like receptor (TLR) proteins which play a large role in pathogen recognition and the activation of innate immunity. Scientists using TLR9 antibodies have found...  Read full blog post.

TLR9: Tollgate to Immunity
Toll-like receptors (TLRs) play an essential role in the activation of innate immunity, and TLRs are expressed in a large number of immune cells as well as in epithelial cells. TLR9 recognizes synthetic oligodeoxynucleotides (ODN) containing unmethyla...  Read full blog post.

TLR9, Infectious Disease and Cancer
Toll-like receptor 9 (TLR9) is a protein encoded by TLR9 gene in humans. It is also known as cluster of differentiation 289 (CD289) and is a member of TLR family. Proteins from TLR family are transmembrane proteins that expressed in both antigen-resen...  Read full blog post.

TLR9 Antibodies in Immunity Research
Toll-like receptor 9 (TLR9) is a member of the toll-like receptor family that plays a key role in pathogen recognition and activation of innate immunity. Scientists using TLR9 antibodies have found the protein is highly conserved from Drosophila to hu...  Read full blog post.

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Recent Reviews

3.7
5
0
4
2
3
1
2
0
1
0

Anonymous
10/20/2017
Application: WB
Species: Human

 
Anonymous
01/14/2015
Application: WB
Species: Human

Mariann Kremlitzka
11/25/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol TLR9
Entrez
Uniprot