TLR9 Antibody (26C593.2)

Images

 
Simple Western: TLR9 Antibody (26C593.2) [NBP2-24729] - Lane view shows a specific band for TLR9 in 0.5 mg/ml of Ramos lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation ...read more
Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR9 protein on epithelial cells. HNEC, Detroit-562 and FaDu were stained intracellularly with PE-Ab against TLR9 (open histograms) or appropriate ...read more
Genetic Strategies: Knockdown Validated: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR-9 protein in HB cells before and after 48 hr of transfection. Beta-actin was used as an internal control. Image ...read more
Immunocytochemistry/ Immunofluorescence: TLR9 Antibody (26C593.2) [NBP2-24729] - Cbl-b is required for the endocytic transit of TLR9. Representative confocal microscopic images of splenocytes from mice with indicated ...read more
Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Analysis of colonic LP or peritoneal cavity (PerC) of normal mice were evaluated by flow cytometry. B cells were intracellularly stained with the anti-TLR9 ...read more
Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Gating strategy for the detection of TLR-7 and -9 in B cell subsets. PBMC were isolated from whole blood and stained for surface markers before cells were fixed, ...read more
Immunohistochemistry-Paraffin: TLR9 Antibody (26C593.2) [NBP2-24729] - Monkey retina tissue. Image from verified customer review.
Western Blot: TLR9 Antibody (26C593.2) [NBP2-24729] - Analysis of TLR9 in A) human PBMC, B) human intestine, C) mouse intestine, and D) rat intestine tissue lysates using this antibody at a dilution of 3 ug/ml.
Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR9 on turbinate epithelial cells from healthy controls compared to turbinate and polyp epithelial cells from patients with CRSwNP, n = 5 (A). ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Eq, Pm, PmSpecies Glossary
Applications WB, Simple Western, DB, ELISA, Flow, Flow-IC, Func, ICC/IF, IHC, IHC-P, In vitro, IP, B/N, CyTOF-ready, KD
Clone
26C593.2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

TLR9 Antibody (26C593.2) Summary

Immunogen
This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-300 of human TLR9 isoform A (Genbank accession no. AAF78037).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TLR9
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-5 ug/ml
  • Simple Western 30 ug/ml
  • Dot Blot
  • ELISA 1:100 - 1:2000
  • Flow Cytometry
  • Functional
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:10 - 1:500
  • In vitro assay
  • Block/Neutralize
  • CyTOF-ready
  • Flow (Intracellular) 1:10 - 1:1000
  • Knockdown Validated
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. In human PBMC, a ~120 kDa band is observed. A smaller isoform, TLR9 isoform B (Genbank accession no. AAF72190) containing 975 amino acids may also be observed in some cases. Use in Functional and in vitro assays reported in scientific literature (PMID 25411258). Use in ELISA reported in multiple publications. Use in Immunoprecipitation reported in scientific literature (PMID 25871979). Use in Flow-intracellular reported in scientific literature (PMID 24986635). Use in functional reported in scientific literature (PMID: 25411258). Use in Dot blot reported in scientific literature (PMID: 27248820). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in Immunohistochemistry reported in scientific literature (PMID 27744078). Use in blocking/ neutralizing reported in scientific literature (PMID: 25338738). Knockdown validation (PMID: 31655343).
Agonist
Reviewed Applications
Read 4 Reviews rated 3.8
using
NBP2-24729 in the following applications:

Publications
Read Publications using
NBP2-24729 in the following applications:

Reactivity Notes

Rhesus Monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for TLR9 Antibody (26C593.2)

  • CD289 antigen
  • CD289
  • TLR9
  • toll-like receptor 9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for TLR9 Antibody (NBP2-24729)(136)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 14 applications: B/N, DB, ELISA, FLOW, Flow, Flow-IC, Func, ICC/IF, IHC, IHC-P, IP, In vitro, KD, WB.


Filter By Application
B/N
(1)
DB
(1)
ELISA
(2)
FLOW
(7)
Flow
(10)
Flow-IC
(2)
Func
(1)
ICC/IF
(10)
IHC
(7)
IHC-P
(10)
IP
(1)
In vitro
(2)
KD
(2)
WB
(13)
All Applications
Filter By Species
Human
(40)
Mouse
(12)
Rat
(1)
Canine
(1)
All Species
Showing Publications 1 - 10 of 136. Show All 136 Publications.
Publications using NBP2-24729 Applications Species
Natarajan S, Ranganathan M Toll-like receptor (TLR) gene expression and immunostimulatory effect of CpG Oligonucleotides in hormone receptor positive cell line T47D and Triple negative breast cancer cell line MDA-MB-468 Immunopharmacol Immunotoxicol Jul 19 2020 [PMID: 32686546]
Zhou B, Yan J, Guo L et al. Hepatoma cell-intrinsic TLR9 activation induces immune escape through PD-L1 upregulation in hepatocellular carcinoma Theranostics May 17 2020 [PMID: 32483468] (WB, IHC, Flow, KD, Human) WB, IHC, Flow, KD Human
Du XK, Ge WY, Jing R, Pan LH Necroptosis in pulmonary macrophages mediates lipopolysaccharide-induced lung inflammatory injury by activating ZBP-1 Int. Immunopharmacol. 2019 Oct 23 [PMID: 31655343] (WB, KD, Mouse) WB, KD Mouse
Kalantari E, Abolhasani M, Roudi R et al. Co-expression of TLR-9 and MMP-13 is associated with the degree of tumour differentiation in prostate cancer Int J Exp Pathol Apr 1 2019 [PMID: 31090157] (Human) Human
Takahara M, Nagato T, Nozaki Y et al A proliferation-inducing ligand (APRIL) induced hyper-production of IgA from tonsillar mononuclear cells in patients with IgA nephropathy Cell. Cell Immunol. 2019 Jul [PMID: 31088610] (Flow, Human)

Details:
Citation using the FITC version of this antibody.
Flow Human
Kapelouzou A, Kontogiannis C, Tsilimigras DI et al. Differential expression patterns of Toll Like Receptors and Interleukin-37 between calcific aortic and mitral valve cusps in humans Cytokine Apr 1 2019 [PMID: 30716659] (IHC, Human) IHC Human
Leppanen J, Helminen O, Huhta H et al. Toll-like receptors 2, 4 and 9 and hypoxia markers HIF-1alpha and CAIX in pancreatic intraepithelial neoplasia.. APMIS. Nov 1 2018 [PMID: 30357962] (IHC, Human) IHC Human
Yang J, Snijders MLH, Haasnoot GW, van Kooten C. Elevated intragraft expression of innate immunity and cell death-related markers is a risk factor for adverse graft outcome. Transpl. Immunol. 2018 Feb 20 [PMID: 29475090] (Human) Human
Chandler MR, Keene KS, Tuomela JM, Forero-Torres A et al. Lower frequency of TLR9 variant associated with protection from breast cancer among African Americans PLoS ONE 2017 Sep 08 [PMID: 28886076] (Human) Human
Sun Y, Xiao Q, Luo C et al. High-glucose induces tau hyperphosphorylation through activation of TLR9-P38MAPK pathway Exp. Cell Res. 2017 Aug 09 [PMID: 28803064] (WB) WB
Show All 136 Publications.

Reviews for TLR9 Antibody (NBP2-24729) (4) 3.84

Average Rating: 3.8
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, macaca mulatta.

Reviews using NBP2-24729:
Filter by Applications
IHC-P
(1)
WB
(3)
All Applications
Filter by Species
Human
(3)
macaca mulatta
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin TLR9 NBP2-24729
Enlarge
4
reviewed by:
Monica Sauter
IHC-P macaca mulatta 04/12/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedparaffin embedded macaque retina tissue
Speciesmacaca mulatta
LotA-6

Comments

CommentsHeat mediated antigen retrieval (pH 6 citrate buffer)
1:25 dilution of primary antibody ON at 4C
1:400 dilution of goat anti-mouse Alexa Fluor 488
Western Blot TLR9 NBP2-24729
Enlarge
3
reviewed by:
Anonymous
WB Human 10/20/2017
View

Summary

ApplicationWestern Blot
Sample Testedhuman lymphocytes, whole cell lysate
SpeciesHuman
LotA-1

Comments

Comments3ug/mL in Milk-TBS/T
30ug of protein, 4-12% Bis-Tris gel
10 minute exposure, several bands. 250, 120, 75, 55kDA
  4
reviewed by:
Anonymous
WB Human 01/14/2015
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate
SpeciesHuman
Lot1005

Blocking

Blocking DetailsBSA Blocked for 1hr

Primary Anitbody

Dilution Ratio1:2500

Details

Detection NotesChemiluminescence ChemiDoc™ XRS+ system, TLR9+ lysate control and TLR9- Lysate Control
Western Blot TLR9 NBP2-24729
Enlarge
4
reviewed by:
Mariann Kremlitzka
WB Human 11/25/2014
View

Summary

ApplicationWestern Blot
Sample TestedPrimary blood-derived B cells
SpeciesHuman
Lot#1005

Blocking

Blocking Details5% milk powder

Primary Anitbody

Dilution Ratio1:3000

Secondary Antibody

Secondary DescriptionGoat anti-rabbit,HRP (DAKO)
Secondary Manufacturer Cat#P0448
Secondary Concentration0,25g/L

Details

Detection NotesPositive control: total B cell lysate, Detection: ECL method (Millipore), 1 minute exposure

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TLR9 Antibody (NBP2-24729). (Showing 1 - 1 of 1 FAQs).

  1. I am a bit unsure about your antibody NBP2-24729 specific against TLR9. I used it for  probing mouse B cell lysate on Proteisimple WES. On their website the recommend your antibody and claim that it detects a specific signal at 90 kDa. But on your website simple western detects a band at 200 kDa?
    • This came during the race to 1000 and we had many people generating and reviewing data.  In retrospect, this was one that probably should not have made it out. As I reviewed the run data, it looks as though the MW ladder did not assign correctly.  When this was corrected, the band at 200 actually shifts higher, up to 280 kDa.  This band now overlaps with the 280 kDa fluorescent standard which is prone to have non-specific interactions with some antibodies.  Those two things make this non-supportive in Simple Western.   We ran Raji lysate on the same run.  This antibody detects a very broad band with a peak at ~150-165 kDa, closer to expected MW.  However, the baseline for this samples was also very high.  If we were presented with this data set today, it would not be considered supportive.  The other information on the Protein Simple web site likely came from a customer. 

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-24729AF350
Alexa Fluor 405 NBP2-24729AF405
Alexa Fluor 488 NBP2-24729AF488
Alexa Fluor 532 NBP2-24729AF532
Alexa Fluor 594 NBP2-24729AF594
Alexa Fluor 647 NBP2-24729AF647
Alexa Fluor 700 NBP2-24729AF700
Alexa Fluor 750 NBP2-24729AF750
Allophycocyanin NBP2-24729APC
Biotin NBP2-24890
DyLight 350 NBP2-24729UV
DyLight 405 NBP2-24729V
DyLight 488 NBP2-24729G
DyLight 550 NBP2-24729R
DyLight 594 NBP2-24729DL594
DyLight 650 NBP2-24729C
DyLight 680 NBP2-24729FR
DyLight 755 NBP2-24729IR
FITC NBP2-24729F
HRP NBP2-24729H
Janelia Fluor 549 NBP2-24729JF549
Janelia Fluor 646 NBP2-24729JF646
PE NBP2-24907
PerCP NBP2-24729PCP

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Blogs on TLR9.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

Topics in CD11b: The innate immune response
Integrins are transmembrane receptors composed of alpha and beta chains, where beta-integrins are mainly expressed in leukocytes. Leukocytes are white blood cells that act in the immune system to defend our body against foreign pathogens.  Integrin...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

TLR9: For Whom the Cell Tolls
The Toll-like receptor 9 (TLR9) protein, also known as CD289, belongs to the family of Toll-like receptor (TLR) proteins which play a large role in pathogen recognition and the activation of innate immunity. Scientists using TLR9 antibodies have found...  Read full blog post.

TLR9: Tollgate to Immunity
Toll-like receptors (TLRs) play an essential role in the activation of innate immunity, and TLRs are expressed in a large number of immune cells as well as in epithelial cells. TLR9 recognizes synthetic oligodeoxynucleotides (ODN) containing unmethyla...  Read full blog post.

TLR9, Infectious Disease and Cancer
Toll-like receptor 9 (TLR9) is a protein encoded by TLR9 gene in humans. It is also known as cluster of differentiation 289 (CD289) and is a member of TLR family. Proteins from TLR family are transmembrane proteins that expressed in both antigen-resen...  Read full blog post.

TLR9 Antibodies in Immunity Research
Toll-like receptor 9 (TLR9) is a member of the toll-like receptor family that plays a key role in pathogen recognition and activation of innate immunity. Scientists using TLR9 antibodies have found the protein is highly conserved from Drosophila to hu...  Read full blog post.

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Recent Reviews

3.8
5
0
4
3
3
1
2
0
1
0

Monica Sauter
04/12/2018
Application: IHC-P
Species: macaca mulatta

Anonymous
10/20/2017
Application: WB
Species: Human

 
Anonymous
01/14/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol TLR9
Uniprot