TLR9 Antibody (26C593.2)

Simple Western: TLR9 Antibody (26C593.2) [NBP2-24729] - Lane view shows a specific band for TLR9 in 0.5 mg/ml of Ramos lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system. Image using the Azide Free format of this antibody.

Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR9 protein on epithelial cells. HNEC, Detroit-562 and FaDu were stained intracellularly with PE-Ab against TLR9 (open histograms) or appropriate isotype control (shaded histograms) and analyzed by flow cytometry. Representative pictures from one out of three independent experiments are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0098239), licensed under a CC-BY licence.

Knockdown Validated: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR-9 protein in HB cells before and after 48 hr of transfection. Beta-actin was used as an internal control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092748) licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: TLR9 Antibody (26C593.2) [NBP2-24729] - Cbl-b is required for the endocytic transit of TLR9. Representative confocal microscopic images of splenocytes from mice with indicated genotypes. For experiments, cells were stimulated through the BCR (green) for 30 minutes then fixed and stained for TLR9 (blue) and Lamp-1 (red)(n = 3). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0089792) licensed under a CC-BY licence.

Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Analysis of colonic LP or peritoneal cavity (PerC) of normal mice were evaluated by flow cytometry. B cells were intracellularly stained with the anti-TLR9 antibody after the cell surface staining with anti-B220 and CD5 antibodies, and examined using flow cytometry. N = 3, performed twice. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0146191) licensed under a CC-BY licence.

Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Gating strategy for the detection of TLR-7 and -9 in B cell subsets. PBMC were isolated from whole blood and stained for surface markers before cells were fixed, permeabilised and stained for TLR-9. FSC and SSC were first used to gate out debris and SSC-A and SSC-H was utilized to eliminate duplicates. Further gating was done on CD45 and CD19, to target B cells. To separate between the different B cell populations, we gated on CD27 and IgD (D), followed by TLR-9 expression on these subsets. Data from one representative patient is shown. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0120383) licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: TLR9 Antibody (26C593.2) [NBP2-24729] - Monkey retina tissue. Image from verified customer review.

Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR9 on turbinate epithelial cells from healthy controls compared to turbinate and polyp epithelial cells from patients with CRSwNP, n = 5 (A). Intracellular staining for TLR9 (open histogram, black line) and isotype control (filled histogram) on turbinate epithelial cells from a healthy control (B), turbinate epithelial cells (C) and polyp epithelial cells from a patient (D), analysed using flow cytometry. Results are presented as mean +/- SEM, **P<0.01. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0105618) licensed under a CC-BY licence.

• Reactivity: Hu, Mu, Rt, Ca, Eq, Pm, Pm
• Applications: WB, Simple Western, DB, ELISA, Flow, Flow-IC, Func, ICC/IF, IHC, IHC-P, In vitro, IP, B/N, CyTOF-ready, KD
• Clone: 26C593.2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TLR9 Antibody (26C593.2) Summary

• Immunogen: This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-300 of human TLR9 isoform A (Genbank accession no. AAF78037).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TLR9
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2-5 ug/ml
  • Simple Western 30 ug/ml
  • Dot Blot
  • ELISA 1:100 - 1:2000
  • Flow Cytometry
  • Functional
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:10 - 1:500
  • In vitro assay
  • Block/Neutralize
  • CyTOF-ready
  • Flow (Intracellular) 1:10 - 1:1000
  • Knockdown Validated
• Application Notes: Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. In human PBMC, a ~120 kDa band is observed. A smaller isoform, TLR9 isoform B (Genbank accession no. AAF72190) containing 975 amino acids may also be observed in some cases. Use in Functional and in vitro assays reported in scientific literature (PMID 25411258). Use in ELISA reported in multiple publications. Use in Immunoprecipitation reported in scientific literature (PMID 25871979). Use in Flow-intracellular reported in scientific literature (PMID 24986635). Use in functional reported in scientific literature (PMID: 25411258). Use in Dot blot reported in scientific literature (PMID: 27248820). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in Immunohistochemistry reported in scientific literature (PMID 27744078). Use in blocking/ neutralizing reported in scientific literature (PMID: 25338738). Knockdown validation (PMID: 31655343).
• Agonist:
  • CpG oligodeoxynucleotides with negative control, TLR9 ligand NBP2-26232

Reactivity Notes

Rhesus Monkey.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for TLR9 Antibody (26C593.2)

• CD289 antigen
• CD289
• TLR9
• toll-like receptor 9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TLR9 Antibody (NBP2-24729)(133)

Publications using NBP2-24729

• Du XK, Ge WY, Jing R, Pan LH Necroptosis in pulmonary macrophages mediates lipopolysaccharide-induced lung inflammatory injury by activating ZBP-1 Int. Immunopharmacol. 2019 Oct 23 [PMID: 31655343] (WB, KD, Mouse)
• Kalantari E, Abolhasani M, Roudi R et al. Co-expression of TLR-9 and MMP-13 is associated with the degree of tumour differentiation in prostate cancer Int J Exp Pathol Apr 1 2019 [PMID: 31090157] (Human)
• Takahara M, Nagato T, Nozaki Y et al A proliferation-inducing ligand (APRIL) induced hyper-production of IgA from tonsillar mononuclear cells in patients with IgA nephropathy Cell. Cell Immunol. 2019 Jul [PMID: 31088610] (Flow, Human)
  
  Details:
  Citation using the FITC version of this antibody.
• Kapelouzou A, Kontogiannis C, Tsilimigras DI et al. Differential expression patterns of Toll Like Receptors and Interleukin-37 between calcific aortic and mitral valve cusps in humans Cytokine Apr 1 2019 [PMID: 30716659] (IHC, Human)
• Leppanen J, Helminen O, Huhta H et al. Toll-like receptors 2, 4 and 9 and hypoxia markers HIF-1alpha and CAIX in pancreatic intraepithelial neoplasia.. APMIS. Nov 1 2018 [PMID: 30357962] (IHC, Human)
• Yang J, Snijders MLH, Haasnoot GW, van Kooten C. Elevated intragraft expression of innate immunity and cell death-related markers is a risk factor for adverse graft outcome. Transpl. Immunol. 2018 Feb 20 [PMID: 29475090] (Human)
• Chandler MR, Keene KS, Tuomela JM, Forero-Torres A et al. Lower frequency of TLR9 variant associated with protection from breast cancer among African Americans PLoS ONE 2017 Sep 08 [PMID: 28886076] (Human)
• Sun Y, Xiao Q, Luo C et al. High-glucose induces tau hyperphosphorylation through activation of TLR9-P38MAPK pathway Exp. Cell Res. 2017 Aug 09 [PMID: 28803064] (WB)
• Heftrig D, Sturm R, Oppermann E et al. Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients. Mediators Inflamm. [PMID: 28255201] (Flow, Human)
  
  Details:
  Citation using the Alexa Fluor 647 form of this antibody.
• Larsson O, Tengroth L, Xu Y et al. Substance P represents a novel first-line defense mechanism in the nose. J Allergy Clin Immunol. [PMID: 28219705] (Flow, ICC/IF, IHC, Mouse)
  
  Details:
  Citation using the Alexa Fluor 647 form of this antibody.
• Leppanen J, Helminen O, Huhta H et al. High toll-like receptor (TLR) 9 expression is associated with better prognosis in surgically treated pancreatic cancer patients. Virchows Arch. Feb 12 2017 [PMID: 28191612] (IHC, Human)
• Helminen O, Huhta H, Kauppila JH et al. Localization of nucleic acid-sensing toll-like receptors in human and mouse pancreas. APMIS. Dec 28 2016 [PMID: 28028829] (IHC, Human)
• Lim JS, Kim HS, Nguyen KC, Cho KA. The role of TLR9 in stress-dependent autophagy formation. Biochem Biophys Res Commun 2016 Dec 09 [PMID: 27793667] (Human)
• Mu L, Wang Y, Wang Y et al. Tumor Location and Survival Outcomes in Adult Patients with Supratentorial Glioblastoma by Levels of Toll-like Receptor 9 Expression. World Neurosurg. Oct 12 2016 [PMID: 27744078] (IHC, Human)
• Burgueno JF, Barba A, Eyre E et al. TLR2 and TLR9 modulate enteric nervous system inflammatory responses to lipopolysaccharide. J Neuroinflammation. Aug 19 2016 [PMID: 27538577] (ICC/IF, Rat)
• Meseure D, Vacher S, Drak Alsibai K et al. Biopathological Significance of TLR9 Expression in Cancer Cells and Tumor Microenvironment Across Invasive Breast Carcinomas Subtypes Cancer Microenviron 2016 Jul 09 [PMID: 27392414] (IHC-P, Human)
• Schnerch J, Prasse A, Vlachakis D et al. Functional TLR9 Expression and CXCR3 Ligand Release in Pulmonary Sarcoidosis Am. J. Respir. Cell Mol. Biol. 2016 Jul 08 [PMID: 27390897]
• Huhta H, Helminen O, Kauppila JH et al. The Expression of Toll-like Receptors in Normal Human and Murine Gastrointestinal Organs and the Effect of Microbiome and Cancer J. Histochem. Cytochem. 2016 Aug 01 [PMID: 27370795] (IHC-P, Human)
• Jeon YK, Kim CK, Koh J et al. Pellino-1 confers chemoresistance in lung cancer cells by upregulating cIAP2 through Lys63-mediated polyubiquitination. Oncotarget. Jul 5 2016 [PMID: 27248820] (DB, Human)
• Duhamel M, Rodet F, Murgoci AN et al. The proprotein convertase PC1/3 regulates TLR9 trafficking and the associated signaling pathways. Sci Rep. 2016 Jan 18 [PMID: 26778167] (WB, Mouse)
• Hamidi S. Molecular mechanisms of the dermal immune response: cooperation between Toll-like receptors and Sphingosine-1-phosphate in fibroblasts. Thesis. 2014 (ICC/IF, Human)
  
  Details:
  TLR9 antibody used for ICC-IF in experiments involving primary cultures of normal human dermal fibroblasts isolated from human juvenile foreskin.
• Siponen M, Bitu CC, Al-Samadi A et al. Cathepsin k expression is increased in oral lichen planus. J. Oral Pathol. Med. May 6 2016 [PMID: 27152719]
• Moreira ML, Costa-Pereira C, Alves MLR. Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes. Veterinary Parasitology [PMID: 26995719] (FLOW, Canine)
  
  Details:
  Used the PE form of this antibody.
• Acharya M, Sokolovska A, Tam JM et al. alphav Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells. Nat Commun. 2016 Mar 11 [PMID: 26965188] (FLOW, Mouse)
• Evangelista MG, Castro SB, Alves CC et al. Early IFN-gamma production together with decreased expression of TLR3 and TLR9 characterizes EAE development conditional on the presence of myelin. Autoimmunity [PMID: 26911613] (FLOW, Mouse)
  
  Details:
  Used the PE form of this antibody.
• Dillmann C, Ringel C, Ringleb J et al. S1PR4 Signaling Attenuates ILT 7 Internalization To Limit IFN-alpha Production by Human Plasmacytoid Dendritic Cells J Immunol 2016 Jan 18 [PMID: 26783340] (Flow, Human)
  
  Details:
  This citation used the PE version of this antibody.
• Mishima Y, Ishihara S, Oka A et al. Decreased Frequency of Intestinal Regulatory CD5+ B Cells in Colonic Inflammation. PLoS ONE. 2016 Jan 05 [PMID: 26727001] (FLOW, Mouse)
• Zambirinis Constantinos P, Levie Elliot, Nguy Susanna et al. TLR9 ligation in pancreatic stellate cells promotes tumorigenesis. Journal of Experimental Medicine 2015 [PMID: 26481685] (Flow, Mouse)
  
  Details:
  This citation used the Biotin version of this antibody.
• Banus JFB. Understanding the role of Toll-like receptors in the lower gastrointestinal tract. Thesis. 2014 (IHC-P, Mouse)
  
  Details:
  Citation using the Biotin form of this antibody.
• Jamin A, Dehoux L, Dossier C et al. Toll-like receptor 3 expression and function in childhood idiopathic nephrotic syndrome Clin Exp Immunol 2015 Jun 30 [PMID: 26123900] (Flow, Human)
  
  Details:
  This citation used the FITC version of this antibody.
• Saito K, Kukita K, Kutomi G et al. Heat shock protein 90 associates with Toll-like receptors 7/9 and mediates self-nucleic acid recognition in SLE Eur. J. Immunol. 2015 Apr 13 [PMID: 25871979] (WB, IP, Human)
• Huhta H, Helminen O, Kauppila JH et al. Toll-like receptor 9 expression in the natural history of Barrett mucosa Virchows Arch. 2015 Apr 03 [PMID: 25838081] (IHC-P, Human)
  
  Details:
  TLR9 antibody was used for IHC-P application on formalin-fixed and paraffin-embedded specimens from human subjects with normal esophagus, gastric or intestinal metaplasia without dysplasia, and low-grade or high-grade dysplasia and esophageal adenocarcinoma. The IHC-P assay involved HIER-microwaves with Tris-EDTA pH 9 buffer for 15 minutes, permeablization with 1 % Triton X-100 in PBS for 5 minutes, use of primary antibody at 1:150 dilution, detection with Dako Envision kit -DAB as well as Alexa conjugated secondary antibodies.
• Karlsen M, Hansen T, Nordal HH et al. Expression of Toll-Like Receptor -7 and -9 in B Cell Subsets from Patients with Primary Sjogren's Syndrome. PLoS ONE 2015 Mar 20 [PMID: 25790192] (FLOW, Human)
  
  Details:
  Using the Alexa Fluor (R) 647 conjugated version of NBP2-24729, catalog number NBP2-24729AF647.
• Yu Sl, Wong Ck, Szeto Cc et al. Members of the receptor for advanced glycation end products axis as potential therapeutic targets in patients with lupus nephritis Lupus et al. 2014 Nov 18 [PMID: 25411258] (Func, In vitro, Human)
  
  Details:
  TLR9 antibody used at 1ug/ml concentration for blocking/neutrilization or functional experiments on in vitro cultured monocytes from human lupus patients.
• Kauppila JH, Korvala J, Siirila K et al. Toll-like receptor 9 mediates invasion and predicts prognosis in squamous cell carcinoma of the mobile tongue. J. Oral Pathol. Med. 2014 Oct 23 [PMID: 25338738] (IHC-P, B/N, Human)
  
  Details:
  TLR9 antibody (clone 26C593.2) used for IHC-P on human squamous cell carcinoma of the tongue with normal tongue as a control (Figure 4A-C). Antibody also used for blocking/neutrilization in experiment involving wound healing model of f HSC-3 cells (Figure 2).
• Bharti D, Kumar A, Mahla RS et al. The role of TLR9 polymorphism in susceptibility to pulmonary tuberculosis. Immunogenetics. 2014 Sep 25 [PMID: 25248338] (FLOW, Human)
  
  Details:
  TLR9 antibody used for FLOW application on Peripheral blood mononuclear cells / PBMCs (from healthy tribal people in India) fixed in 1 % PFA/15 minutes at RT - Fixation blocked with 2 % FCS-PBS, Cells permeablized with 0.1 % Triton-X 100-PBS for 15 min at RT, Washed 2X with PBS, Incubated with primary at 1 ug/10X6 cells conc for 30 min followed by antimouse secondary antibody Alexa-Fluor-488, washed again and reconstituted in 500 ul PBS followed by analysis on BD FACS Aria III (Fig 1).
• Tengroth L, Arebro J, Kumlien Georen S et al. Deprived TLR9 Expression in Apparently Healthy Nasal Mucosa Might Trigger Polyp-Growth in Chronic Rhinosinusitis Patients. PLoS ONE 2014 Aug 19 [PMID: 25133733] (FLOW, Human)
  
  Details:
  Using the Alexa Fluor (R) 647 conjugated version of NBP2-24729, catalog number NBP2-24729AF647.
• Fehri E, Ennaifer E, Ardhaoui M et al. Expression of Toll-like Receptor 9 Increases with Progression of Cervical Neoplasia in Tunisian Women - A Comparative Analysis of Condyloma, Cervical Intraepithelial Neoplasia and Invasive Carcinoma. Asian Pac. J Cancer Prev. 2014 Aug 15 [PMID: 25124588] (IHC-P, Human)
• Lopez MC, Palmer BE, Lawrence DA. Naive T cells, unconventional NK and NKT cells, and highly responsive monocyte-derived macrophages characterize human cord blood Immunobiology 2014 Jun 11 [PMID: 24986635] (Flow-IC, Human)
  
  Details:
  This citation used the PE version of this antibody.
• Ruan M, Thorn K, Liu S et al. The secretion of IL-6 by CpG-ODN-treated cancer cells promotes T-cell immune responses partly through the TLR-9/AP-1 pathway in oral squamous cell carcinoma. Int. J. Oncol. 2014 Jun 01 [PMID: 24676671] (WB, Human)
  
  Details:
  Human oral squamous carcinoma cell line.
• Ruan M, Zhang Z, Li S et al. Activation of Toll-Like Receptor-9 Promotes Cellular Migration via Up-Regulating MMP-2 Expression in Oral Squamous Cell Carcinoma. PLoS ONE 2014 Mar 24 [PMID: 24658023] (WB, Human)
  
  Details:
  HB oral squamous cell carcinoma cells, Fig 4A. The specificity of the TLR9 mAb was validated in TLR9 siRNA transfected HB cells by WB (Fig 4A).
• Veselits M, Tanaka A, Lipkowitz S et al. Recruitment of Cbl-b to B Cell Antigen Receptor Couples Antigen Recognition to Toll-Like Receptor 9 Activation in Late Endosomes. PLoS ONE 2014 Mar 21 [PMID: 24651487] (ICC/IF, Mouse)
  
  Details:
  Mouse splenocytes, Figs 6A, S3 IF (confocal micrscopy).
• Guerrier T, Pochard P, Lahiri A et al. TLR9 expressed on plasma membrane acts as a negative regulator of human B cell response. J. Autoimmun. 2014 Feb 25 [PMID: 24582318] (In vitro, Human)
  
  Details:
  Human B cells: The TLR9 mAb was used to stimulate cells, Figs 4, 5.
• Zufferey A, Schvartz D, Nolli S et al. Characterization of the platelet granule proteome: Evidence of the presence of MHC1 in alpha-granules. J Proteomics. 2014 Feb 16 [PMID: 24549006] (ICC/IF, Human)
  
  Details:
  Resting platelets, Fig 3A. TLR9 is platelet a T granule marker, and the TLR9 mAb was used to detect T granules.
• Korvala J, Harjula T, Siirila K et al. Toll-like receptor 9 expression in mucoepidermoid salivary gland carcinoma may associate with good prognosis. J. Oral Pathol. Med. 2014 Feb 3 [PMID: 24484266] (WB, IHC-P, Human)
  
  Details:
  IHC (P): mucoepidermoid carcinoma (MEC) (Fig 2), UT-MUC-1 organotypic cultures (Fig 3); WB: UT-MUC-l (Fig 4). TLR4 was detected as 3 bands at ~130 kDa and as 100 and 80 kDa cleavage fragments (Fig 4).
• Hamidi S, Schafer-Korting M, Weindl G et al. TLR2/1 and sphingosine 1-phosphate modulate inflammation, myofibroblast differentiation and cell migration in fibroblasts. Biochim. Biophys. Acta. 2014 Jan 15 [PMID: 24440818] (ICC/IF, Human)
  
  Details:
  Dermal fibroblasts, data described but not shown.
• Sathe A, Reddy KV. TLR9 and RIG-I signaling in human endocervical epithelial cells modulates inflammatory responses of macrophages and dendritic cells in vitro. PLoS ONE. 2014 Jan 10 [PMID: 24409285] (WB, Flow-IC, ICC/IF, Human)
  
  Details:
  End1/E6E7 endocervical epithelial cells: Flow (Intracellular), Fig 1C; IF (confocal microscopy), Fig 1D; WB, Figs S1A, S1C. Both CpG-ODN and Poly(I:C) stimulation upregulated TLR9 expression. TLR9 was detected at ~100 kDa in WB. Mouse monoclonal against T
• Chandel HS, Pandey SP, Shukla D et al. TLRs and CD40 modulate each others expression affecting Leishmania major infection. Clin. Exp. Immunol. 2014 Jan 6 [PMID: 24387292] (WB, Mouse)
  
  Details:
  Mouse macrophages, Fig 2a. TLR9 antibody.
• Sasaki K, Tanaka A, Nagatomo H, Ogawa H. Characterization of Toll-Like Receptor 9 expression during Mouse Preimplantation Development. J Genit Syst Disor 5: 1. J Genit Syst Disor. Jan 15 2016 (WB, ICC/IF, Mouse)
• Belmont L, Rabbe N, Antoine M et al. Expression of TLR9 in tumor-infiltrating mononuclear cells enhances angiogenesis and is associated with a worse survival in lung cancer. Int J Cancer. 2013 Aug 3 [PMID: 23913633]
• Vaisanen MR, Jukkola-Vuorinen A, Vuopala KS et al. Expression of Toll-like receptor-9 is associated with poor progression-free survival in prostate cancer. Oncol Lett. 2013 May [PMID: 23761830]
• Figueiredo MM, Amorim IF, Pinto AJ et al. Expression of Toll-like receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum. BMC Immunol. 2013 May 14 [PMID: 23668673]
• Abdi J, Mutis T, Garssen J, Redegeld F. Characterization of the Toll-like receptor expression profile in human multiple myeloma cells. PLoS One. 2013 Apr 8 [PMID: 23593278]
• Chauhan SK, Singh VV, Rai R et al. Distinct autoantibody profiles in systemic lupus erythematosus patients are selectively associated with TLR7 and TLR9 upregulation. J Clin Immunol. 2013 Jul [PMID: 23564191]
• Schneberger D, Caldwell S, Kanthan R, Singh B. Expression of Toll-like receptor 9 in mouse and human lungs. J Anat. 2013 May [PMID: 23521717]
• Hu F, Zhang L, Zheng J et al. Spontaneous production of immunoglobulin M in human epithelial cancer cells. PLoS One. 2012 [PMID: 23251529]
• Harman AN, Bye CR, Nasr N et al. Identification of lineage relationships and novel markers of blood and skin human dendritic cells. J Immunol. 2013 Jan 1 [PMID: 23183897]
• Kauppila JH, Karttunen TJ, Saarnio J et al. Short DNA sequences and bacterial DNA induce esophageal, gastric, and colorectal cancer cell invasion. APMIS. 2013 Jun [PMID: 23082743]
• Siponen M, Kauppila JH, Soini Y, Salo T. TLR4 and TLR9 are induced in oral lichen planus. J Oral Pathol Med. 2012 Nov [PMID: 22672741]
• Souza-Fonseca-Guimaraes F, Parlato M, Fitting C et al. NK cell tolerance to TLR agonists mediated by regulatory T cells after polymicrobial sepsis. J Immunol. 2012 Jun 15 [PMID: 22566566]
• Silver AC, Arjona A, Walker WE, Fikrig E. The circadian clock controls toll-like receptor 9-mediated innate and adaptive immunity. Immunity. 2012 Feb 24 [PMID: 22342842]
• O'Hara JR, Feener TD, Fischer CD, Buret AG. Campylobacter jejuni disrupts protective Toll-like receptor 9 signaling in colonic epithelial cells and increases the severity of dextran sulfate sodium-induced colitis in mice. Infect Immun. 2012 Apr [PMID: 22311925]
• Zuo D, Yu X, Guo C et al. Molecular chaperoning by glucose-regulated protein 170 in the extracellular milieu promotes macrophage-mediated pathogen sensing and innate immunity. FASEB J. 2012 Apr [PMID: 22207611]
• Kauppila Joonas H, Takala Heikki, Selander Katri S et al. Increased Toll-like receptor 9 expression indicates adverse prognosis in oesophageal adenocarcinoma. Histopathology. 2011 Oct [PMID: 22014045] (Human)
• Ronkainen Hanna, Hirvikoski Pasi, Kauppila Saila et al. Absent Toll-like receptor-9 expression predicts poor prognosis in renal cell carcinoma. J Exp Clin Cancer Res. 2011 [PMID: 21929816] (Human)
• Luckhardt Tracy R, Coomes Stephanie M, Trujillo Glenda et al. TLR9-induced interferon beta is associated with protection from gammaherpesvirus-induced exacerbation of lung fibrosis. Fibrogenesis Tissue Repair. 2011 [PMID: 21810214] (ICC/IF)
• Lundeen Jeff S, Sutherland Brandon, Patel Aabid et al. Direct measurement of the quantum wavefunction. Nature. 2011 Jun 9 [PMID: 21654800] (ELISA)
• Karim R, Meyers C, Backendorf C et al. Human papillomavirus deregulates the response of a cellular network comprising of chemotactic and proinflammatory genes. PLoS One. 2011 Mar 14 [PMID: 21423754]
• Dasgupta G, Chentoufi AA, You S et al. Engagement of TLR2 reverses the suppressor function of conjunctiva CD4+CD25+ regulatory T cells and promotes herpes simplex virus epitope-specific CD4+CD25- effector T cell responses. Invest Ophthalmol Vis Sci. 2011 May 17 [PMID: 21273544]
• van Gent M, Griffin BD, Berkhoff EG et al. EBV lytic-phase protein BGLF5 contributes to TLR9 downregulation during productive infection. J Immunol. 2011 Feb 1 [PMID: 21191071]
• Myles A, Aggarwal A. Expression of Toll-like receptors 2 and 4 is increased in peripheral blood and synovial fluid monocytes of patients with enthesitis-related arthritis subtype of juvenile idiopathic arthritis. Rheumatology (Oxford). 2011 Mar [PMID: 21097451]
• Baumann Christoph L, Aspalter Irene M, Sharif Omar et al. CD14 is a coreceptor of Toll-like receptors 7 and 9. J Exp Med. 2010 Nov 22 [PMID: 21078886] (ELISA)
• Schneberger D, Lewis D, Caldwell S, Singh B. Expression of toll-like receptor 9 in lungs of pigs, dogs and cattle. Int J Exp Pathol. 2011 Feb [PMID: 21044185]
• Sethi RS, Brar RS, Singh O, Singh B. Immunolocalization of pulmonary intravascular macrophages, TLR4, TLR9 and IL-8 in normal and Pasteurella multocida-infected lungs of water buffalo (Bubalus bubalis). J Comp Pathol. 2011 Feb-Apr [PMID: 20880542]
• Wang C, Cao S, Yan Y et al. TLR9 expression in glioma tissues correlated to glioma progression and the prognosis of GBM patients. BMC Cancer. 2010 Aug 10 [PMID: 20696081]
• Booth JS, Arsenault R, Napper S et al. TLR9 signaling failure renders Peyer's patch regulatory B cells unresponsive to stimulation with CpG oligodeoxynucleotides. J Innate Immun. 2010 [PMID: 20551621]
• Keestra AM, de Zoete MR, Bouwman LI, van Putten JP. Chicken TLR21 is an innate CpG DNA receptor distinct from mammalian TLR9. J Immunol. 2010 Jul 1 [PMID: 20498358]
• Jukkola-Vuorinen A, Rahko E, Vuopala KS et al. Toll-like receptor-9 expression is inversely correlated with estrogen receptor status in breast cancer. J Innate Immun. 2009 [PMID: 20375566]
• Machida H, Ito S, Hirose T et al. Expression of Toll-like receptor 9 in renal podocytes in childhood-onset active and inactive lupus nephritis. Nephrol Dial Transplant. 2010 Aug [PMID: 20181802]
• Kim YI, Park JE, Brand DD et al. Protein kinase D1 is essential for the proinflammatory response induced by hypersensitivity pneumonitis-causing thermophilic actinomycetes Saccharopolyspora rectivirgula. J Immunol. 2010 Mar 15 [PMID: 20142359]
• Panda A, Qian F, Mohanty S et al. Age-associated decrease in TLR function in primary human dendritic cells predicts influenza vaccine response. J Immunol. 2010 Mar 1 [PMID: 20100933]
• Chentoufi AA, Dasgupta G, Nesburn AB et al. Nasolacrimal duct closure modulates ocular mucosal and systemic CD4(+) T-cell responses induced following topical ocular or intranasal immunization. Clin Vaccine Immunol. 2010 Mar [PMID: 20089796]
• Vaisanen Marja-Riitta, Vaisanen Timo, Jukkola-Vuorinen Arja et al. Expression of toll-like receptor-9 is increased in poorly differentiated prostate tumors. Prostate. 2010 Jun 1 [PMID: 20054821] (Human)
• Wu J, Meng Z, Jiang M et al. Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific. Immunology. 2010 Mar [PMID: 19922426]
• Schneberger David, Caldwell Sarah, Suri Sarabjeet Singh, Singh Baljit. Expression of toll-like receptor 9 in horse lungs. Anat Rec (Hoboken). 2009 Jul [PMID: 19548205] (WB)
• Faruk EM,Yousef MM, Mohamed T. Does vitamin D have protective effect on human nasal polyposis: histological and immunohistochemical study J Am Coll Cardiol 2009 Feb 10 [PMID: 19195606] (IHC-P, Human)
  
  Details:
  This reference used the Azide Free version of NBP2-24729.
• Zhou M, McFarland-Mancini MM, Funk HM et al. Toll-like receptor expression in normal ovary and ovarian tumors. Cancer Immunol Immunother. 2009 Sep [PMID: 19184006]
• Donaghy H, Bosnjak L, Harman AN et al. Role for plasmacytoid dendritic cells in the immune control of recurrent human herpes simplex virus infection. J Virol. 2009 Feb [PMID: 19073735]
• Palladino MA, Savarese MA, Chapman JL et al. Localization of Toll-like receptors on epididymal epithelial cells and spermatozoa. Am J Reprod Immunol. 2008 Dec [PMID: 19032616]
• Sacre SM, Lo A, Gregory B et al. Inhibitors of TLR8 reduce TNF production from human rheumatoid synovial membrane cultures. J Immunol. 2008 Dec 1 [PMID: 19017992]
• Ilvesaro Joanna M, Merrell Melinda A, Li Li et al. Toll-like receptor 9 mediates CpG oligonucleotide-induced cellular invasion. Mol Cancer Res. 2008 Oct [PMID: 18922969] (ICC/IF)
• Cohen PA, Koski GK, Czerniecki BJ et al. STAT3- and STAT5-dependent pathways competitively regulate the pan-differentiation of CD34pos cells into tumor-competent dendritic cells. Blood. 2008 Sep 1 [PMID: 18577706]
• Selleri S, Palazzo M, Deola S et al. Induction of pro-inflammatory programs in enteroendocrine cells by the Toll-like receptor agonists flagellin and bacterial LPS. Int Immunol. 2008 Aug [PMID: 18544573]
• Ju X, Zenke M, Hart DN, Clark GJ. CD300a/c regulate type I interferon and TNF-alpha secretion by human plasmacytoid dendritic cells stimulated with TLR7 and TLR9 ligands. Blood. 2008 Aug 15 [PMID: 18535206]
• Hammadi A, Billard C, Faussat AM et al. Stimulation of iNOS expression and apoptosis resistance in B-cell chronic lymphocytic leukemia (B-CLL) cells through engagement of Toll-like receptor 7 (TLR-7) and NF-kappaB activation. Nitric Oxide. 2008 Sep [PMID: 18474259]
• Burgener IA, Jungi TW. Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol. 2008 Jul 15 [PMID: 18439687]
• Nakamura K, Miyazato A, Xiao G et al. Deoxynucleic acids from Cryptococcus neoformans activate myeloid dendritic cells via a TLR9-dependent pathway. J Immunol. 2008 Mar 15 [PMID: 18322216]
• Sipola S. Colectomy In An Icu Patient Population Thesis 2014 (IHC-P, Human)
  
  Details:
  This reference used the Azide Free version of NBP2-24729.
• Meneghin A, Choi ES, Evanoff HL et al. TLR9 is expressed in idiopathic interstitial pneumonia and its activation promotes in vitro myofibroblast differentiation. Histochem Cell Biol. 2008 Nov [PMID: 18633634]
• Faruk EM,Yousef MM, Mohamed T. Does vitamin D have protective effect on human nasal polyposis: histological and immunohistochemical study. Journal of Histology & Histopathology. 2014 (IHC-P, Human)
• Zhang YW, Davis EG, Blecha F, Wilkerson MJ. Molecular cloning and characterization of equine Toll-like receptor 9. Vet Immunol Immunopathol. 2008 Aug 15 [PMID: 18462806]
• Cognasse F, Hamzeh-Cognasse H, Lafarge S et al. Identification of two subpopulations of purified human blood B cells, CD27- CD23+ and CD27high CD80+, that strongly express cell surface Toll-like receptor 9 and secrete high levels of interleukin-6. Immunology. 2008 Nov [PMID: 18445007]
• Pegu A, Qin S, Fallert Junecko BA et al. Human lymphatic endothelial cells express multiple functional TLRs. J Immunol. 2008 Mar 1 [PMID: 18292566]
• Meng Y, Kujas M, Marie Y et al. Expression of TLR9 within human glioblastoma. J Neurooncol. 2008 May [PMID: 18253698]
• Nakano S, Morimoto S, Suzuki J et al. Role of pathogenic auto-antibody production by Toll-like receptor 9 of B cells in active systemic lupus erythematosus. Rheumatology (Oxford). 2008 Feb [PMID: 18160420]
• Kundu Shilajit D, Lee Chung, Billips Benjamin K et al. The toll-like receptor pathway: a novel mechanism of infection-induced carcinogenesis of prostate epithelial cells. Prostate. 2008 Feb 1 [PMID: 18092352]
• Gonzalez JM, Xu H, Ofori E, Elovitz MA. Toll-like receptors in the uterus, cervix, and placenta: is pregnancy an immunosuppressed state? Am J Obstet Gynecol. 2007 Sep [PMID: 17826427]
• Tran N, Koch A, Berkels R et al. Toll-like receptor 9 expression in murine and human adrenal glands and possible implications during inflammation. J Clin Endocrinol Metab. 2007 Jul [PMID: 17473064]
• Kindrachuk J, Potter JE, Brownlie R et al. Nucleic acids exert a sequence-independent cooperative effect on sequence-dependent activation of Toll-like receptor 9. J Biol Chem. 2007 May 11 [PMID: 17355967]
• Wong CK, Cheung PF, Ip WK, Lam CW. Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils. Am J Respir Cell Mol Biol. 2007 Jul [PMID: 17332440]
• Bauer JMJ. Interferon-b moduliert die Expression von Toll-like Rezeptoren in mononuklearen Blutzellen und induziert Toll-like Rezeptor 7 in plasmazytoiden dendritischen Zellen von Patienten mit Multipler Sklerose. thesis. 2015 Nov 12
• Ewaschuk JB, Backer JL, Churchill TA et al. Surface expression of Toll-like receptor 9 is upregulated on intestinal epithelial cells in response to pathogenic bacterial DNA. Infect Immun. 2007 May [PMID: 17325049]
• Palladino MA, Johnson TA, Gupta R et al. Members of the Toll-like receptor family of innate immunity pattern-recognition receptors are abundant in the male rat reproductive tract. Biol Reprod. 2007 Jun [PMID: 17314314]
• Lee J, Mo JH, Katakura K et al. Maintenance of colonic homeostasis by distinctive apical TLR9 signalling in intestinal epithelial cells. Nat Cell Biol. 2006 Dec [PMID: 17128265]
• Dhara A, Saini M, Das DK et al. Molecular characterization of coding sequences and analysis of Toll-like receptor 3 mRNA expression in water buffalo (Bubalus bubalis) and nilgai (Boselaphus tragocamelus). Immunogenetics. 2007 Jan [PMID: 17119952]
• Niessner A, Sato K, Chaikof EL et al. Pathogen-sensing plasmacytoid dendritic cells stimulate cytotoxic T-cell function in the atherosclerotic plaque through interferon-alpha. Circulation. 2006 Dec 5 [PMID: 17116765]
• Huggins J, Pellegrin T, Felgar RE et al. CpG DNA activation and plasma-cell differentiation of CD27- naive human B cells Blood 2007 Feb 15 [PMID: 17032927] (Flow)
  
  Details:
  This citation used the FITC version of this antibody.
• Wang H, Nicholas MW, Conway KL et al. EBV latent membrane protein 2A induces autoreactive B cell activation and TLR hypersensitivity J Immunol 2006 Sep 1 [PMID: 16920914] (Flow)
  
  Details:
  This citation used the FITC version of this antibody.
• Mansson A, Adner M, Cardell LO. Toll-like receptors in cellular subsets of human tonsil T cells: altered expression during recurrent tonsillitis. Respir Res. 2006 Feb 27 [PMID: 16504163]
• Gottenberg JE, Cagnard N, Lucchesi C et al. Activation of IFN pathways and plasmacytoid dendritic cell recruitment in target organs of primary Sjogren's syndrome. Proc Natl Acad Sci U S A. 2006 Feb 21 [PMID: 16477017]
• Martin-Armas M, Simon-Santamaria J, Pettersen I et al. Toll-like receptor 9 (TLR9) is present in murine liver sinusoidal endothelial cells (LSECs) and mediates the effect of CpG-oligonucleotides. J Hepatol. 2006 May [PMID: 16458386]
• Lee J, Wu CC, Lee KJ et al. Activation of anti-hepatitis C virus responses via Toll-like receptor 7. Proc Natl Acad Sci U S A. 2006 Feb 7 [PMID: 16446426]
• Damiano V, Caputo R, Bianco R et al. Novel toll-like receptor 9 agonist induces epidermal growth factor receptor (EGFR) inhibition and synergistic antitumor activity with EGFR inhibitors. Clin Cancer Res. [PMID: 16428503]
  
  Details:
  Citation using the Azide Free form of this antibody.
• Tabeta K, Hoebe K, Janssen EM et al. The Unc93b1 mutation 3d disrupts exogenous antigen presentation and signaling via Toll-like receptors 3, 7 and 9. Nat Immunol. 2006 Feb [PMID: 16415873]
• Srivastava MD, Srivastava BI. Expression of mRNA and proteins for toll-like receptors, associated molecules, defensins and LL-37 by SRIK-NKL, a CD8+ NK/T cell line. Leuk Res. 2005 Jul [PMID: 15927677]
• Miller LS, SOrensen OE, Liu PT et al. TGF-alpha regulates TLR expression and function on epidermal keratinocytes. J Immunol. 2005 May 15 [PMID: 15879109]
• Abel K, Wang Y, Fritts L et al. Deoxycytidyl-deoxyguanosine oligonucleotide classes A, B, and C induce distinct cytokine gene expression patterns in rhesus monkey peripheral blood mononuclear cells and distinct alpha interferon responses in TLR9-expressing rhesus monkey plasmacytoid dendritic cells. Clin Diagn Lab Immunol. 2005 May [PMID: 15879022]
• Cognasse F, Hamzeh H, Chavarin P et al. Evidence of Toll-like receptor molecules on human platelets. Immunol Cell Biol. 2005 Apr [PMID: 15748217]
• Greene CM, Carroll TP, Smith SG et al. TLR-induced inflammation in cystic fibrosis and non-cystic fibrosis airway epithelial cells. J Immunol. 2005 Feb 1 [PMID: 15661927]
• Droemann D, Albrecht D, Gerdes J et al. Human lung cancer cells express functionally active Toll-like receptor 9. Respir Res. 2005 Jan 4 [PMID: 15631627]
• Zhong F, Cao W, Chan E et al. Deviation from major codons in the Toll-like receptor genes is associated with low Toll-like receptor expression Immunology 2005 Jan [PMID: 15606798] (Flow)
  
  Details:
  This citation used the FITC version of this antibody.
• Lee HK, Dunzendorfer S, Tobias PS. Cytoplasmic domain-mediated dimerizations of toll-like receptor 4 observed by beta-lactamase enzyme fragment complementation. J Biol Chem. 2004 Mar 12 [PMID: 14699116]
• Gantner Florian, Hermann Patrice, Nakashima Kosuke et al. CD40-dependent and -independent activation of human tonsil B cells by CpG oligodeoxynucleotides. Eur J Immunol. 2003 Jun [PMID: 12778475]

Reviews for TLR9 Antibody (NBP2-24729) (4)

Average Rating: 3.8

(Based on 4 reviews)

We have 4 reviews tested in 2 species: Human, macaca mulatta.

reviewed by: Monica Sauter IHC-P macaca mulatta 04/12/2018

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: paraffin embedded macaque retina tissue
• Species: macaca mulatta
• Lot: A-6

Comments

• Comments: Heat mediated antigen retrieval (pH 6 citrate buffer)
  1:25 dilution of primary antibody ON at 4C
  1:400 dilution of goat anti-mouse Alexa Fluor 488

reviewed by: Anonymous WB Human 10/20/2017

Summary

• Application: Western Blot
• Sample Tested: human lymphocytes, whole cell lysate
• Species: Human
• Lot: A-1

Comments

• Comments: 3ug/mL in Milk-TBS/T
  30ug of protein, 4-12% Bis-Tris gel
  10 minute exposure, several bands. 250, 120, 75, 55kDA

reviewed by: Anonymous WB Human 01/14/2015

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysate
• Species: Human
• Lot: 1005

Blocking

• Blocking Details: BSA Blocked for 1hr

Primary Anitbody

• Dilution Ratio: 1:2500

Details

• Detection Notes: Chemiluminescence ChemiDoc™ XRS+ system, TLR9+ lysate control and TLR9- Lysate Control

reviewed by: Mariann Kremlitzka WB Human 11/25/2014

Summary

• Application: Western Blot
• Sample Tested: Primary blood-derived B cells
• Species: Human
• Lot: #1005

Blocking

• Blocking Details: 5% milk powder

Primary Anitbody

• Dilution Ratio: 1:3000

Secondary Antibody

• Secondary Description: Goat anti-rabbit,HRP (DAKO)
• Secondary Manufacturer Cat#: P0448
• Secondary Concentration: 0,25g/L

Details

• Detection Notes: Positive control: total B cell lysate, Detection: ECL method (Millipore), 1 minute exposure

FAQs for TLR9 Antibody (NBP2-24729). (Showing 1 - 1 of 1 FAQs).

1. I am a bit unsure about your antibody NBP2-24729 specific against TLR9. I used it for  probing mouse B cell lysate on Proteisimple WES. On their website the recommend your antibody and claim that it detects a specific signal at 90 kDa. But on your website simple western detects a band at 200 kDa?
   • This came during the race to 1000 and we had many people generating and reviewing data.  In retrospect, this was one that probably should not have made it out. As I reviewed the run data, it looks as though the MW ladder did not assign correctly.  When this was corrected, the band at 200 actually shifts higher, up to 280 kDa.  This band now overlaps with the 280 kDa fluorescent standard which is prone to have non-specific interactions with some antibodies.  Those two things make this non-supportive in Simple Western.   We ran Raji lysate on the same run.  This antibody detects a very broad band with a peak at ~150-165 kDa, closer to expected MW.  However, the baseline for this samples was also very high.  If we were presented with this data set today, it would not be considered supportive.  The other information on the Protein Simple web site likely came from a customer. 

Bioinformatics

• Gene Symbol: TLR9
• Uniprot:
  • Q9NR96()