Mouse IL-10 DuoSet ELISA, 15 Plate



Product Details

Reactivity MuSpecies Glossary
Applications ELISA

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Mouse IL-10 DuoSet ELISA, 15 Plate Summary

Assay Type
Solid Phase Sandwich ELISA
See PDF Datasheet for details
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details


Application Notes
No significant interference observed with available related molecules.
Read Publications using DY417.

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-10 DuoSet ELISA, 15 Plate

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF


Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 alpha -helical cytokine family that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 (1-3). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (4, 5). Mature mouse IL-10 shares 85% amino acid sequence identity with rat IL-10 and 70-77% with bovine, canine, equine, feline, human, ovine, and porcine IL-10. It contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently-associated homodimer (4, 6, 7). 
IL-10 mediates its biological activities through a heteromeric receptor complex composed of the type II cytokine receptor subunits IL-10 R alpha and IL-10 R beta . IL-10 R alpha is a 110 kDa transmembrane glycoprotein that is expressed on lymphocytes, NK cells, macrophages, monocytes, astrocytes, intestinal epithelial cells, cytotrophoblasts, and activated hepatic stellate cells (8-13), while the 75 kDa transmembrane IL-10 R beta is widely expressed (14, 15). The IL-10 dimer binds to two IL 10 R alpha chains, triggering recruitment of two IL-10 R beta chains (14, 15). IL-10 R beta does not bind IL-10 directly but is required for signal transduction. IL-10 R beta also associates with IL-20 R alpha , IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (16-18). 
The involvement of IL-10 in immunoregulation includes both suppressive and stimulatory effects. It functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells (19-21) and by promoting the development of M2 macrophages (21). Its expression by immunosuppressive regulatory T cells (Treg) and regulatory B cells is important for Treg proliferation (19). Within a tumor microenvironment, however, IL-10 inhibits the expansion of Treg as well as myeloid-derived suppressor cells (22, 23). IL-10 induces the intratumoral accumulation and activation of CD8+ T cells (24, 25). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation (19) and promoting muscle regeneration after injury (21), but it also contributes to the persistence of viral infections (26). The levels of IL-10 are elevated in Sjogren's syndrome (saliva), primary CNS lymphoma (cerebrospinal fluid), and ovarian cancer (serum and ascites) (27-29). Its levels are decreased in the serum in patients with recurrent heart attacks or during preeclampsia and also in the seminal fluid of infertile men (30-32).

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Publications for IL-10 (DY417)(223)

We have publications tested in 3 confirmed species: Human, Mouse, Gerbil.

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Showing Publications 1 - 10 of 223. Show All 223 Publications.
Publications using DY417 Applications Species
JR Santin, L Benvenutti, MF Broering, R Nunes, FC Goldoni, YBK Patel, JA de Souza, MAT Kopp, P de Souza, RCV da Silva, MVD Pastor, AB de Souza, LD Testoni, AG Couto, TMB Bresolin, NLM Quintão Sambucus nigra: A traditional medicine effective in reducing inflammation in mice Journal of ethnopharmacology, 2021;283(0):114736. 2021 [PMID: 34648899] (Mouse) Mouse
G Dunsmore, EP Rosero, S Shahbaz, DM Santer, J Jovel, P Lacy, S Houston, S Elahi Neutrophils promote T-cell activation through the regulated release of CD44-bound Galectin-9 from the cell surface during HIV infection PloS Biology, 2021;19(8):e3001387. 2021 [PMID: 34411088] (Human) Human
L Benvenutti, R Nunes, I Venturi, SA Ramos, MF Broering, FC Goldoni, SE Pavan, MVD Pastor, A Malheiros, NLM Quintão, ES Fernandes, JR Santin Anti-Inflammatory and Healing Activity of the Hydroalcoholic Fruit Extract of Solanum diploconos (Mart.) Bohs Journal of Immunology Research, 2021;2021(0):9957451. 2021 [PMID: 34337087] (Mouse) Mouse
S Jittimanee, S Wongratana, C Kaewraemru, J Jittimanee Opisthorchis viverrini antigens up-regulates the expression of CD80 and MHC class II in JAWSII mouse dendritic cells and promotes IL-10 and TGF-&beta secretions Parasitology international, 2021;84(0):102401. 2021 [PMID: 34082134] (Mouse) Mouse
J Zhu, T Inomata, K Fujimoto, K Uchida, K Fujio, K Nagino, M Miura, N Negishi, Y Okumura, Y Akasaki, K Hirosawa, M Kuwahara, A Eguchi, H Shokirova, A Yanagawa, A Midorikawa, A Murakami Ex Vivo-Induced Bone Marrow-Derived Myeloid Suppressor Cells Prevent Corneal Allograft Rejection in Mice Investigative Ophthalmology & Visual Science, 2021;62(7):3. 2021 [PMID: 34061951] (Mouse) Mouse
A Hadi, A Rastgoo, M Eskandaria, N Haghighipo, A Bolhassani Development of Delivery Systems Enhances the Potency of Cell-Based HIV-1 Therapeutic Vaccine Candidates Journal of Immunology Research, 2021;2021(0):5538348. 2021 [PMID: 33997055] (Mouse) Mouse
J Wu, N Chen, Y Liu, G Godlewski, HJ Kaplan, SH Shrader, ZH Song, H Shao Studies of involvement of G-protein coupled receptor-3 in cannabidiol effects on inflammatory responses of mouse primary astrocytes and microglia PLoS ONE, 2021;16(5):e0251677. 2021 [PMID: 33984046] (Mouse) Mouse
JP Martins, LC Silva, MS Nunes, G Rübensam, JR Oliveira, RBM Silva, MM Campos Combined Effects of Exercise and Phytoanabolic Extracts in Castrated Male and Female Mice Nutrients, 2021;13(4):. 2021 [PMID: 33918334] (Mouse) Mouse
D Arsenijevi, B Stojanovic, J Milovanovi, A Arsenijevi, M Simic, M Pergal, I Kodranov, O Cvetkovic, D Vojvodic, E Ristanovic, D Manojlovic, M Milovanovi, N Arsenijevi Hepatoprotective Effect of Mixture of Dipropyl Polysulfides in Concanavalin A-Induced Hepatitis Nutrients, 2021;13(3):. 2021 [PMID: 33809904] (Mouse) Mouse
D Shah, P Das, S Acharya, B Agarwal, DJ Christense, SM Robertson, V Bhandari Small Immunomodulatory Molecules as Potential Therapeutics in Experimental Murine Models of Acute Lung Injury (ALI)/Acute Respiratory Distress Syndrome (ARDS) International Journal of Molecular Sciences, 2021;22(5):. 2021 [PMID: 33806560] (Mouse) Mouse
Show All 223 Publications.

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Product General Protocols

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FAQs for IL-10 (DY417). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Biotin DY417

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Gene Symbol Il10