Mouse IL-10 DuoSet ELISA, 15 Plate

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
Biotin

Order Details

Mouse IL-10 DuoSet ELISA, 15 Plate Summary

Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Il10

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DY417.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-10 DuoSet ELISA, 15 Plate

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • GVHDS
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF

Background

Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 alpha -helical cytokine family that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 (1-3). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (4, 5). Mature mouse IL-10 shares 85% amino acid sequence identity with rat IL-10 and 70-77% with bovine, canine, equine, feline, human, ovine, and porcine IL-10. It contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently-associated homodimer (4, 6, 7). 
IL-10 mediates its biological activities through a heteromeric receptor complex composed of the type II cytokine receptor subunits IL-10 R alpha and IL-10 R beta . IL-10 R alpha is a 110 kDa transmembrane glycoprotein that is expressed on lymphocytes, NK cells, macrophages, monocytes, astrocytes, intestinal epithelial cells, cytotrophoblasts, and activated hepatic stellate cells (8-13), while the 75 kDa transmembrane IL-10 R beta is widely expressed (14, 15). The IL-10 dimer binds to two IL 10 R alpha chains, triggering recruitment of two IL-10 R beta chains (14, 15). IL-10 R beta does not bind IL-10 directly but is required for signal transduction. IL-10 R beta also associates with IL-20 R alpha , IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (16-18). 
The involvement of IL-10 in immunoregulation includes both suppressive and stimulatory effects. It functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells (19-21) and by promoting the development of M2 macrophages (21). Its expression by immunosuppressive regulatory T cells (Treg) and regulatory B cells is important for Treg proliferation (19). Within a tumor microenvironment, however, IL-10 inhibits the expansion of Treg as well as myeloid-derived suppressor cells (22, 23). IL-10 induces the intratumoral accumulation and activation of CD8+ T cells (24, 25). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation (19) and promoting muscle regeneration after injury (21), but it also contributes to the persistence of viral infections (26). The levels of IL-10 are elevated in Sjogren's syndrome (saliva), primary CNS lymphoma (cerebrospinal fluid), and ovarian cancer (serum and ascites) (27-29). Its levels are decreased in the serum in patients with recurrent heart attacks or during preeclampsia and also in the seminal fluid of infertile men (30-32).

Publications for IL-10 (DY417)(287)

We have publications tested in 4 confirmed species: Human, Mouse, Gerbil, Transgenic Mouse.


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Human
(2)
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(281)
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Showing Publications 1 - 10 of 287. Show All 287 Publications.
Publications using DY417 Applications Species
Pereira, RDD;Rabelo, RAN;Oliveira, NFM;Porto, SLT;Andrade, ACDSP;Queiroz-Junior, CM;Barbosa, CLN;de Souza-Costa, LP;Santos, FRDS;Oliveira, FBR;da Silva, BLV;Umezu, HL;Ferreira, R;da Silva, GSF;Cruz, JS;Teixeira, MM;Costa, VV;Machado, FS; A 5-Lipoxygenase Inhibitor, Zileuton, Modulates Host Immune Responses and Improves Lung Function in a Model of Severe Acute Respiratory Syndrome (SARS) Induced by Betacoronavirus Viruses 2023-10-04 [PMID: 37896826]
Heinzl, GC;Eriksen, DB;Johnsen, PR;Scarafoni, A;Frøkiær, H; Protein Concentration Affects the Food Allergen ?-Conglutin Uptake and Bacteria-Induced Cytokine Production in Dendritic Cells Biomolecules 2023-10-16 [PMID: 37892213] (Mouse) Mouse
Russo, S;Kwiatkowski, M;Wolters, JC;Gerding, A;Hermans, J;Govorukhina, N;Bischoff, R;Melgert, BN; Effects of lysine deacetylase inhibitor treatment on LPS responses of alveolar-like macrophages Journal of leukocyte biology 2023-10-09 [PMID: 37811856] (Mouse) Mouse
Su, N;Villicana, C;Zhang, C;Lee, J;Sinha, S;Yang, A;Yang, F; Aspirin synergizes with mineral particle-coated macroporous scaffolds for bone regeneration through immunomodulation Theranostics 2023-08-15 [PMID: 37649612] (Mouse) Mouse
Schädel, P;Wichmann-Costaganna, M;Czapka, A;Gebert, N;Ori, A;Werz, O; Short-Term Caloric Restriction and Subsequent Re-Feeding Compromise Liver Health and Associated Lipid Mediator Signaling in Aged Mice Nutrients 2023-08-21 [PMID: 37630850] (Mouse) Mouse
Yang, Y;Gao, ZF;Hou, GG;Meng, QG;Hou, Y; Discovery of anti-neuroinflammatory agents from 1,4,5,6-tetrahydrobenzo[2,3]oxepino[4,5-d]pyrimidin-2-amine derivatives by regulating microglia polarization European journal of medicinal chemistry 2023-11-05 [PMID: 37544188] (Mouse) Mouse
Kelestemur, T;Németh, ZH;Pacher, P;Beesley, J;Robson, SC;Eltzschig, HK;Haskó, G; Adenosine metabolized from extracellular ATP ameliorates organ injury by triggering A2BR signaling Respiratory research 2023-07-13 [PMID: 37438813] (Mouse) Mouse
Merriman, JA;Xu, W;Caparon, MG; Central carbon flux controls growth/damage balance for Streptococcus pyogenes PLoS pathogens 2023-06-29 [PMID: 37384800] (Mouse) Mouse
Torres, L;Camila Gonçalves Miranda, M;Dantas Martins, V;Caixeta, F;de Almeida Oliveira, M;Martins Trindade, L;Carvalho de Assis, H;Nascimento, V;Pinheiro Rosa, N;Gomes, E;Oliveira Almeida, S;Marquet, F;Genser, L;Marcelin, G;Clément, K;Russo, M;Maria Caetano Faria, A;Uceli Maioli, T; Obesity-induced hyperglycemia impairs oral tolerance induction and aggravates food allergy Mucosal immunology 2023-06-09 [PMID: 37302712] (Mouse) Mouse
Falvey, A;Palandira, SP;Chavan, SS;Brines, M;Tracey, KJ;Pavlov, VA; Electrical stimulation of the dorsal motor nucleus of the vagus regulates inflammation without affecting the heart rate bioRxiv : the preprint server for biology 2023-05-20 [PMID: 37292846] (Mouse) Mouse
Show All 287 Publications.

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CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
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Bioinformatics

Gene Symbol Il10