Mouse IL-10 DuoSet ELISA, 15 Plate

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
Biotin

Order Details

Mouse IL-10 DuoSet ELISA, 15 Plate Summary

Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Il10

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DY417.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-10 DuoSet ELISA, 15 Plate

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • GVHDS
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF

Background

Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 alpha -helical cytokine family that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 (1-3). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (4, 5). Mature mouse IL-10 shares 85% amino acid sequence identity with rat IL-10 and 70-77% with bovine, canine, equine, feline, human, ovine, and porcine IL-10. It contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently-associated homodimer (4, 6, 7). 
IL-10 mediates its biological activities through a heteromeric receptor complex composed of the type II cytokine receptor subunits IL-10 R alpha and IL-10 R beta . IL-10 R alpha is a 110 kDa transmembrane glycoprotein that is expressed on lymphocytes, NK cells, macrophages, monocytes, astrocytes, intestinal epithelial cells, cytotrophoblasts, and activated hepatic stellate cells (8-13), while the 75 kDa transmembrane IL-10 R beta is widely expressed (14, 15). The IL-10 dimer binds to two IL 10 R alpha chains, triggering recruitment of two IL-10 R beta chains (14, 15). IL-10 R beta does not bind IL-10 directly but is required for signal transduction. IL-10 R beta also associates with IL-20 R alpha , IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (16-18). 
The involvement of IL-10 in immunoregulation includes both suppressive and stimulatory effects. It functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells (19-21) and by promoting the development of M2 macrophages (21). Its expression by immunosuppressive regulatory T cells (Treg) and regulatory B cells is important for Treg proliferation (19). Within a tumor microenvironment, however, IL-10 inhibits the expansion of Treg as well as myeloid-derived suppressor cells (22, 23). IL-10 induces the intratumoral accumulation and activation of CD8+ T cells (24, 25). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation (19) and promoting muscle regeneration after injury (21), but it also contributes to the persistence of viral infections (26). The levels of IL-10 are elevated in Sjogren's syndrome (saliva), primary CNS lymphoma (cerebrospinal fluid), and ovarian cancer (serum and ascites) (27-29). Its levels are decreased in the serum in patients with recurrent heart attacks or during preeclampsia and also in the seminal fluid of infertile men (30-32).

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Publications for IL-10 (DY417)(265)

We have publications tested in 3 confirmed species: Human, Mouse, Gerbil.


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Human
(2)
Mouse
(259)
Gerbil
(1)
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Showing Publications 1 - 10 of 265. Show All 265 Publications.
Publications using DY417 Applications Species
V Mulens-Ari, A Nicolás-Bo, F Carn, F Gazeau Cationic Polyethyleneimine (PEI)-Gold Nanocomposites Modulate Macrophage Activation and Reprogram Mouse Breast Triple-Negative MET-1 Tumor Immunological Microenvironment Pharmaceutics, 2022;14(10):. 2022 [PMID: 36297669] (Mouse) Mouse
GJ Wang, X Dong, Y Li Cluster of Differentiation 147 (CD147) serves as a promoter of atherosclerosis in patients with cerebral infarction European review for medical and pharmacological sciences, 2022;26(16):5710-5717. 2022 [PMID: 36066144] (Human) Human
J Won, A Jo, CH Gil, S Kim, H Shin, H Jik Kim Inhaled delivery of recombinant interferon-lambda restores allergic inflammation after development of asthma by controlling Th2- and Th17-cell-mediated immune responses International immunopharmacology, 2022;112(0):109180. 2022 [PMID: 36030690] (Mouse) Mouse
Y Zhang, L Zhu, J Hong, C Chen Extracellular matrix of early pulmonary fibrosis modifies the polarization of alveolar macrophage International immunopharmacology, 2022;111(0):109179. 2022 [PMID: 36029666] (Mouse) Mouse
JT Castro, P Azevedo, MJ Fumagalli, NS Hojo-Souza, N Salazar, GG Almeida, LI Oliveira, L Faustino, LR Antonelli, TG Marçal, M Augusto, B Valiate, A Fiorini, B Rattis, SG Ramos, M Piccin, OC Nonato, L Benevides, R Magalhães, B Cassaro, G Burle, D Doro, J Kalil, E Durigon, A Salazar, O Caballero, H Santiago, A Machado, JS Silva, F da Fonseca, AP Fernandes, SR Teixeira, RT Gazzinelli Promotion of neutralizing antibody-independent immunity to wild-type and SARS-CoV-2 variants of concern using an RBD-Nucleocapsid fusion protein Nature Communications, 2022;13(1):4831. 2022 [PMID: 35977933] (Mouse) Mouse
PHF Sucupira, TR Moura, ILS Gurgel, TTP Pereira, ACB Padovan, MM Teixeira, D Bahia, FM Soriani In vitro and in vivo Characterization of Host-Pathogen Interactions of the L3881 Candida albicans Clinical Isolate Frontiers in Microbiology, 2022;13(0):901442. 2022 [PMID: 35898912] (Mouse) Mouse
M Yin, C Li, L Zhang, L Zhang, J Lin, N Jiang, Q Wang, Q Xu, H Zheng, L Gu, Y Jia, B Yu, G Zhao Mechanism of antifungal activity and therapeutic action of beta-ionone on Aspergillus fumigatus keratitis via suppressing LOX1 and JNK/p38 MAPK activation International immunopharmacology, 2022;110(0):108992. 2022 [PMID: 35810488] (Mouse) Mouse
AM Kelly, JM Leech, SL Doyle, RM McLoughlin Staphylococcus aureus-induced immunosuppression mediated by IL-10 and IL-27 facilitates nasal colonisation PloS Pathogens, 2022;18(7):e1010647. 2022 [PMID: 35776778] (Mouse) Mouse
M Holthaus, N Santhakuma, T Wahlers, A Paunel-Gör The Secretome of Preconditioned Mesenchymal Stem Cells Drives Polarization and Reprogramming of M2a Macrophages toward an IL-10-Producing Phenotype International Journal of Molecular Sciences, 2022;23(8):. 2022 [PMID: 35456922] (Mouse) Mouse
EC Dominguez, R Phandthong, M Nguyen, A Ulu, S Guardado, S Sveiven, P Talbot, TM Nordgren Aspirin-Triggered Resolvin D1 Reduces Chronic Dust-Induced Lung Pathology without Altering Susceptibility to Dust-Enhanced Carcinogenesis Cancers, 2022;14(8):. 2022 [PMID: 35454807] (Mouse) Mouse
Show All 265 Publications.

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Blogs on IL-10.


  Read full blog post.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

How To Identify B Cell Subsets Using Flow Cytometry
By Victoria OsinskiUsing Flow Cytometry to Identify B Cell SubsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified a...  Read full blog post.

PPAR gamma - An important target in human metabolism
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells by interacting with members of the nuclear hormone receptor superfamily known as peroxisome proliferator activated receptors (PPARs). There are f...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

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Bioinformatics

Gene Symbol Il10