Mouse IL-10 DuoSet ELISA, 15 Plate

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
Biotin

Order Details

Mouse IL-10 DuoSet ELISA, 15 Plate Summary

Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Il10

Applications/Dilutions

Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DY417.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-10 DuoSet ELISA, 15 Plate

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • GVHDS
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF

Background

Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 alpha -helical cytokine family that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 (1-3). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (4, 5). Mature mouse IL-10 shares 85% amino acid sequence identity with rat IL-10 and 70-77% with bovine, canine, equine, feline, human, ovine, and porcine IL-10. It contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently-associated homodimer (4, 6, 7). 
IL-10 mediates its biological activities through a heteromeric receptor complex composed of the type II cytokine receptor subunits IL-10 R alpha and IL-10 R beta. IL-10 R alpha is a 110 kDa transmembrane glycoprotein that is expressed on lymphocytes, NK cells, macrophages, monocytes, astrocytes, intestinal epithelial cells, cytotrophoblasts, and activated hepatic stellate cells (8-13), while the 75 kDa transmembrane IL-10 R beta is widely expressed (14, 15). The IL-10 dimer binds to two IL 10 R alpha chains, triggering recruitment of two IL-10 R beta chains (14, 15). IL-10 R beta does not bind IL-10 directly but is required for signal transduction. IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (16-18). 
The involvement of IL-10 in immunoregulation includes both suppressive and stimulatory effects. It functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells (19-21) and by promoting the development of M2 macrophages (21). Its expression by immunosuppressive regulatory T cells (Treg) and regulatory B cells is important for Treg proliferation (19). Within a tumor microenvironment, however, IL-10 inhibits the expansion of Treg as well as myeloid-derived suppressor cells (22, 23). IL-10 induces the intratumoral accumulation and activation of CD8+ T cells (24, 25). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation (19) and promoting muscle regeneration after injury (21), but it also contributes to the persistence of viral infections (26). The levels of IL-10 are elevated in Sjogren's syndrome (saliva), primary CNS lymphoma (cerebrospinal fluid), and ovarian cancer (serum and ascites) (27-29). Its levels are decreased in the serum in patients with recurrent heart attacks or during preeclampsia and also in the seminal fluid of infertile men (30-32).

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Publications for IL-10 (DY417)(188)

We have publications tested in 2 confirmed species: Mouse, Gerbil.


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Mouse
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Gerbil
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Showing Publications 1 - 10 of 188. Show All 188 Publications.
Publications using DY417 Applications Species
BN Nguyen, A Chávez-Arr, MI Cheng, M Krasilniko, A Louie, DA Portnoy TLR2 and endosomal TLR-mediated secretion of IL-10 and immune suppression in response to phagosome-confined Listeria monocytogenes PLoS Pathog., 2020;16(7):e1008622. 2020 [PMID: 32634175] (Mouse) Mouse
KI Lee, JS Bae, EH Kim, JH Kim, L Lyu, YJ Chung, JH Mo Strain-Specific Differences in House Dust Mite (Dermatophagoides farinae)-Induced Mouse Models of Allergic Rhinitis Clin Exp Otorhinolaryngol, 2020;0(0):. 2020 [PMID: 32407614] (Mouse) Mouse
YR Na, JW Kwon, DY Kim, H Chung, J Song, D Jung, H Quan, D Kim, JS Kim, YW Ju, W Han, HS Ryu, YS Lee, JJ Hong, SH Seok Protein Kinase A Catalytic Subunit Is a Molecular Switch that Promotes the Pro-tumoral Function of Macrophages Cell Rep, 2020;31(6):107643. 2020 [PMID: 32402274] (Mouse) Mouse
R Santos, PC Campos, M Rungue, V Rocha, D Santos, V Mendes, FV Marinho, F Martins, MF Ricci, DCD Reis, GD Cassali, JC Alves-Filh, AT Vieira, SC Oliveira The Role of ST2 Receptor in the Regulation of Brucella abortus Oral Infection Pathogens, 2020;9(5):. 2020 [PMID: 32353980] (Mouse) Mouse
HC Lin, JY Lin M1 Polarization but Anti-LPS-Induced Inflammation and Anti-MCF-7 Breast Cancer Cell Growth Effects of Five Selected Polysaccharides Evid Based Complement Alternat Med, 2020;2020(0):9450246. 2020 [PMID: 32308723] (Mouse) Mouse
C Eckstein, JPS Mol, FB Costa, PP Nunes, PA Lima, MM Melo, TP Carvalho, DO Santos, MF Silva, TF Carvalho, LF Costa, OAO Melo Júnio, RC Giunchette, TA Paixão, RL Santos Brucella ovis mutant in ABC transporter protects against Brucella canis infection in mice and it is safe for dogs PLoS ONE, 2020;15(4):e0231893. 2020 [PMID: 32298378] (Mouse) Mouse
AP Bonfanti, N Barreto, J Munhoz, M Caballero, G Cordeiro, T Rocha-E-Si, R Sutti, F Moura, S Brunetto, CD Ramos, R Thomé, L Verinaud, C Rapôso Spider venom administration impairs glioblastoma growth and modulates immune response in a non-clinical model Sci Rep, 2020;10(1):5876. 2020 [PMID: 32246025] (Mouse) Mouse
H Karimi, H Soleimanja, A Abdoli, RS Banijamali Combination therapy using human papillomavirus L1/E6/E7 genes and archaeosome: a nanovaccine confer immuneadjuvanting effects to fight cervical cancer Sci Rep, 2020;10(1):5787. 2020 [PMID: 32238821] (Mouse) Mouse
RDC da Cunha d, MM Cruz, TM de Farias, VS da Silva, J de Jesus S, MM Telles, MIC Alonso-Val Fish oil reverses metabolic syndrome, adipocyte dysfunction, and altered adipokines secretion triggered by high-fat diet-induced obesity Physiol Rep, 2020;8(4):e14380. 2020 [PMID: 32109344] (Mouse) Mouse
N Taghipour, N Mosaffa, HA Aghdaei, M Rostami-Ne, JV Weinstock, S Shahnavaz, MR Zali Immunomodulatory effect of Syphacia obvelata in treatment of experimental DSS-induced colitis in mouse model Sci Rep, 2019;9(1):19127. 2019 [PMID: 31836772] (Mouse) Mouse
Show All 188 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-10 (DY417). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

Biotin DY417
Biotin DY417

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Blogs on IL-10.

COVID-19-Related Neurological Problems: Uncovering the Mechanisms
By Jamshed Arslan, Pharm D, PhDThere is good news and bad news related to COVID-19, a disease caused by SARS-COV-2 infection. Good news first: the vast majority of otherwise healthy individuals remain asymptomatic...  Read full blog post.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

How to identify B cell subsets using flow cytometry
By Victoria OsinskiUsing flow cytometry to identify B cell subsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified a...  Read full blog post.

PPAR gamma - An important target in human metabolism
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells by interacting with members of the nuclear hormone receptor superfamily known as peroxisome proliferator activated receptors (PPARs). There are f...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

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Bioinformatics

Gene Symbol Il10