TLR1 Antibody

Images

 
Flow Cytometry: TLR1 Antibody [NB100-56563] - TLR1 expression pattern in primary BMNCs from 3 MM patients analyzed by flow cytometry. CD138-positive cells were gated from the total cell population. Staining for was ...read more
Western Blot: TLR1 Antibody [NB100-56563] - analysis of TLR1 in Ramos (A), Raw (B), and (C) TLR1 transfected cell lysate using this antibody. goat anti-rabbit HRP secondary antibody was used.
Orthogonal Strategies: Western Blot: TLR1 Antibody [NB100-56563] - All the cell lines expressed mRNA for TLR1 (Figure 1) and displayed a strong expression of TLR1 protein (Figure 2). Cell lysates were ...read more
Flow Cytometry: TLR1 Antibody [NB100-56563] - Expression of TLR1 in Fravel, L363, OPM1, RPMI8226, XG1, and NCI-H929 as determined by flow cytometry. HCMLs were stained using an intracellular staining protocol with TLR1 ...read more
Immunohistochemistry-Paraffin: TLR1 Antibody [NB100-56563] - analysisof human small intestine using this antibody at 10 ug/ml.
Flow Cytometry: TLR1 Antibody [NB100-56563] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56563. Staining of TLR1 in human PBMCs (lymphocyte-gated) using this antibody at 10 ul/10^6 cells. Shaded histogram ...read more
Flow Cytometry: TLR1 Antibody [NB100-56563] - Intracellular analysis of TLR1 AF647 in human PBMCs (lymphocyte-gated) using this antibody at 10 ul/10^6 cells. Shaded histogram represents cells without antibody, green ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, DB, Flow, Flow-CS, Flow-IC, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
       

Orthogonal Strategies

 

Order Details

TLR1 Antibody Summary

Immunogen
This antibody was developed against a KLH-conjugated synthetic peptide corresponding to a sequence within amino acids 400-450 of human TLR1.
Specificity
In Ramos, an 87 kDa band is observed.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TLR1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dot Blot
  • Flow (Cell Surface)
  • Flow (Intracellular)
  • Flow Cytometry 2-4 ug/ 1x10-6 cells
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1-3 ug/ml
Application Notes
Flow (Cell Surface): 0.5-1 ug/10^6 cells, or see Wong et al, 2007 Flow (Intracellular): 0.5-1 ug/10^6 cells. Use in Immunohistochemistry reported in scientific literature (PMID 27008696). Use in Dot blot reported in scientific literature (PMID: 27248820). Use in Flow-intracellular reported in scientific literature (PMID: 23593278). Use in FLOW cell surface reported in scientific literature (PMID: 23593278).
Agonist
Publications
Read Publications using
NB100-56563 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TLR1 Antibody

  • CD281 antigen
  • CD281
  • DKFZp547I0610
  • DKFZp564I0682
  • KIAA0012MGC126311
  • MGC104956
  • MGC126312
  • rsc786
  • TIL
  • TIL. LPRS5
  • TLR1
  • Toll/interleukin-1 receptor-like protein
  • toll-like receptor 1

Background

The Toll-like receptor (TLR) family in mammals are comprised of a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain. The TLRs could constitute an important and unrecognized component of innate immunity in humans. In mice, TLR1 interacts with TLR2 and coexpression of TLR1 and TLR2 enhance the NF-kappaB activation in response to a synthetic lipopeptide. They recognize the lipid configuration of the native mycobacterial lipoprotein as well as several triacylated lipopeptides.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56722
Species: Ca, Hu, Mu, Rb, Rt
Applications: B/N, DB, ELISA, Flow-CS, Flow, Func, ICC/IF, IP, In vitro, WB
202-IL
Species: Hu
Applications: BA
NB100-56566
Species: Bv, Hu, Ma, Mu, Po, Rt
Applications: B/N, ChIP, CyTOF-ready, DB, Dual ISH-IHC, ELISA(Cap), ELISA, Flow-CS, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, KD, KO, PAGE, WB
NB100-56536
Species: Hu
Applications: Flow-CS, Flow-IC, Flow, IHC, IHC-P, WB
NBP2-24729
Species: Ca, Eq, Hu, Pm, Mu, Pm, Rt
Applications: B/N, CyTOF-ready, DB, ELISA, Flow-IC, Flow, Func, ICC/IF, IHC, IHC-P, IP, In vitro, KD, Simple Western, WB
D6050
Species: Hu
Applications: ELISA
NBP2-24875
Species: Ca, Hu, Mu
Applications: BA, B/N, Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP2-24787
Species: Ca, Hu, Mu
Applications: DB, Flow-CS, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP1-49045
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
NBP2-24906
Species: Hu, Mu, Rt
Applications: BA, DB, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, WB
NBP1-19371
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
DY417
Species: Mu
Applications: ELISA
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
DDX0490P-100
Species: Hu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
6507-IL/CF
Species: Hu
Applications: BA
NB100-56583
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
NBP2-24917
Species: Hu, Mu, Rb
Applications: CyTOF-ready, DB, Flow-CS, Flow-IC, Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
Supplier Logo

Publications for TLR1 Antibody (NB100-56563)(13)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: DB, FLOW, Flow-IC, IHC, IHC-P, WB.


Filter By Application
DB
(1)
FLOW
(1)
Flow-IC
(1)
IHC
(1)
IHC-P
(1)
WB
(6)
All Applications
Filter By Species
Human
(5)
Mouse
(1)
Rat
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB100-56563 Applications Species
Li Y, Chen X, Jin R et al. Injectable hydrogel with MSNs/microRNA-21-5p delivery enables both immunomodification and enhanced angiogenesis for myocardial infarction therapy in pigs Science advances Feb 3 2021 [PMID: 33627421] (WB, Mouse) WB Mouse
Shang L, Deng D, Roffel S, Gibbs S Differential influence of Streptococcus mitis on host response to metals in reconstructed human skin and oral mucosa Contact Derm. Jul 16 2020 [PMID: 32677222] (WB, Human) WB Human
Huhta H, Helminen O, Kauppila JH et al. The Expression of Toll-like Receptors in Normal Human and Murine Gastrointestinal Organs and the Effect of Microbiome and Cancer J. Histochem. Cytochem. 2016 Aug 01 [PMID: 27370795] (IHC-P, Human) IHC-P Human
Jeon YK, Kim CK, Koh J et al. Pellino-1 confers chemoresistance in lung cancer cells by upregulating cIAP2 through Lys63-mediated polyubiquitination. Oncotarget. Jul 5 2016 [PMID: 27248820] (DB, Human) DB Human
Huhta H, Helminen O, Lehenkari PP et al. Toll-like receptors 1, 2, 4 and 6 in esophageal epithelium, Barrett's esophagus, dysplasia and adenocarcinoma. Oncotarget Apr 26 2016 [PMID: 27008696] (IHC, Human) IHC Human
Regan T, Nally K, Carmody R et al. Identification of TLR10 as a Key Mediator of the Inflammatory Response to Listeria monocytogenes in Intestinal Epithelial Cells and Macrophages. J Immunol 2013 Dec 15 [PMID: 24198280] (FLOW) FLOW
Abdi J, Mutis T, Garssen J, Redegeld F. Characterization of the Toll-like receptor expression profile in human multiple myeloma cells. PLoS One. 2013 Apr 8 [PMID: 23593278] (WB, Flow-IC)

Details:
Antibodies cited [multiple myeloma (MM) cell lines, human intestine tissue lysate, PBMC, mouse splenocytes] for WB (Figs 2, 3, 4A, 4B, 5, Table 1) and Flow (Intracellular): Figs 4C, 6-8, Table 1: 1. TLR1 (IMG-5012) 2. TLR2 (IMG-416A)3. TLR3 (IMG-315A)4 TL
WB, Flow-IC
Stridh L, Smith PL, Naylor AS et al. Regulation of toll-like receptor 1 and -2 in neonatal mice brains after hypoxia-ischemia. J Neuroinflammation. 2011 May 10 [PMID: 21569241]
Bansal K, Elluru SR, Narayana Y et al. PE_PGRS antigens of Mycobacterium tuberculosis induce maturation and activation of human dendritic cells. J Immunol. 2010 Apr 1 [PMID: 20176745] (WB)

Details:
WB in Fig 4B, 4C using HEk-293 cells transiently transfected with TLR2 or TLR2 dominant negative constructs: 1. TLR1 (IMG-5012), 2. TLR2 IMG-(6320A), 3. TLR4 (IMG-577), 4.TLR6 (IMG-527). Note: TLR2 was transfected validated in Fig 4B.
WB
Wu J, Meng Z, Jiang M et al. Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific. Immunology. 2010 Mar [PMID: 19922426]
Show All 13 Publications.

Reviews for TLR1 Antibody (NB100-56563) (0)

There are no reviews for TLR1 Antibody (NB100-56563). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TLR1 Antibody (NB100-56563) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional TLR1 Products

Bioinformatics Tool for TLR1 Antibody (NB100-56563)

Discover related pathways, diseases and genes to TLR1 Antibody (NB100-56563). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TLR1 Antibody (NB100-56563)

Discover more about diseases related to TLR1 Antibody (NB100-56563).
 

Pathways for TLR1 Antibody (NB100-56563)

View related products by pathway.

PTMs for TLR1 Antibody (NB100-56563)

Learn more about PTMs related to TLR1 Antibody (NB100-56563).
 

Research Areas for TLR1 Antibody (NB100-56563)

Find related products by research area.

Blogs on TLR1.

Toll-like receptor 2 activation contributes to oral squamous cell carcinoma development and miRNA-mediated drug resistance
By Jamshed Arslan, Pharm. D., PhD. Squamous cell carcinoma is the most common cancer in the oral cavity.1 The tumor surface biofilms in oral cancers contain high levels of aerobic and anaerobic microorganisms.1,2 Peri...  Read full blog post.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TLR1
TLR1 belongs to the Toll-like receptor (TLR) family, and is a key player in the recognition of pathogens as well as the activation of the innate immunity system. TLRs are highly conserved proteins with a high degree of structural and functional homolo...  Read full blog post.

Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TLR1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol TLR1
Entrez
Uniprot