TLR1 Antibody

Images

 
Western Blot: TLR1 Antibody [NB100-56563] - analysis of TLR1 in Ramos (A), Raw (B), and (C) TLR1 transfected cell lysate using this antibody. goat anti-rabbit HRP secondary antibody was used.
Immunohistochemistry-Paraffin: TLR1 Antibody [NB100-56563] - analysisof human small intestine using this antibody at 10 ug/ml.
Flow Cytometry: TLR1 Antibody [NB100-56563] - Intracellular analysis of TLR1 AF647 in human PBMCs (lymphocyte-gated) using this antibody at 10 ul/10^6 cells. Shaded histogram represents cells without antibody, green ...read more
Flow Cytometry: TLR1 Antibody [NB100-56563] - Cell surface analysis of TLR1 in human PBMCs (monocyte-gated) using this antibody at 0.5 ug/10^6 cells. Shaded histogram represents cells without antibody, green represents ...read more
Flow Cytometry: TLR1 Antibody [NB100-56563] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56563. Staining of TLR1 in human PBMCs (lymphocyte-gated) using this antibody at 10 ul/10^6 cells. Shaded histogram ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, DB, Flow, Flow-IC, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

TLR1 Antibody Summary

Immunogen
This antibody was developed against a KLH-conjugated synthetic peptide corresponding to a sequence within amino acids 400-450 of human TLR1.
Specificity
In Ramos, an 87 kDa band is observed.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TLR1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Dot Blot
  • Flow Cytometry 2-4 ug/ 1x10-6 cells
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Flow (Intracellular)
Application Notes
Flow (Cell Surface): 0.5-1 ug/10^6 cells, or see Wong et al, 2007 Flow (Intracellular): 0.5-1 ug/10^6 cells. Use in Immunohistochemistry reported in scientific literature (PMID 27008696). Use in Dot blot reported in scientific literature (PMID: 27248820). Use in Flow-intracellular reported in scientific literature (PMID: 23593278).
Agonist
Control
TLR1 Overexpression Lysate
Publications
Read Publications using
NB100-56563 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TLR1 Antibody

  • CD281 antigen
  • CD281
  • DKFZp547I0610
  • DKFZp564I0682
  • KIAA0012MGC126311
  • MGC104956
  • MGC126312
  • rsc786
  • TIL
  • TIL. LPRS5
  • TLR1
  • Toll/interleukin-1 receptor-like protein
  • toll-like receptor 1

Background

The Toll-like receptor (TLR) family in mammals are comprised of a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain. The TLRs could constitute an important and unrecognized component of innate immunity in humans. In mice, TLR1 interacts with TLR2 and coexpression of TLR1 and TLR2 enhance the NF-kappaB activation in response to a synthetic lipopeptide. They recognize the lipid configuration of the native mycobacterial lipoprotein as well as several triacylated lipopeptides.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for TLR1 Antibody (NB100-56563)(11)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 6 applications: DB, FLOW, Flow-IC, IHC, IHC-P, WB.


Filter By Application
DB
(1)
FLOW
(1)
Flow-IC
(1)
IHC
(1)
IHC-P
(1)
WB
(4)
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Filter By Species
Human
(4)
Rat
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-56563 Applications Species
Huhta H, Helminen O, Kauppila JH et al. The Expression of Toll-like Receptors in Normal Human and Murine Gastrointestinal Organs and the Effect of Microbiome and Cancer J. Histochem. Cytochem. 2016 Aug 01 [PMID: 27370795] (IHC-P, Human) IHC-P Human
Jeon YK, Kim CK, Koh J et al. Pellino-1 confers chemoresistance in lung cancer cells by upregulating cIAP2 through Lys63-mediated polyubiquitination. Oncotarget. Jul 5 2016 [PMID: 27248820] (DB, Human) DB Human
Huhta H, Helminen O, Lehenkari PP et al. Toll-like receptors 1, 2, 4 and 6 in esophageal epithelium, Barrett's esophagus, dysplasia and adenocarcinoma. Oncotarget Apr 26 2016 [PMID: 27008696] (IHC, Human) IHC Human
Regan T, Nally K, Carmody R et al. Identification of TLR10 as a Key Mediator of the Inflammatory Response to Listeria monocytogenes in Intestinal Epithelial Cells and Macrophages. J Immunol 2013 Dec 15 [PMID: 24198280] (FLOW) FLOW
Abdi J, Mutis T, Garssen J, Redegeld F. Characterization of the Toll-like receptor expression profile in human multiple myeloma cells. PLoS One. 2013 Apr 8 [PMID: 23593278] (WB, Flow-IC)

Details:
Antibodies cited [multiple myeloma (MM) cell lines, human intestine tissue lysate, PBMC, mouse splenocytes] for WB (Figs 2, 3, 4A, 4B, 5, Table 1) and Flow (Intracellular): Figs 4C, 6-8, Table 1: 1. TLR1 (IMG-5012) 2. TLR2 (IMG-416A)3. TLR3 (IMG-315A)4 TL
WB, Flow-IC
Stridh L, Smith PL, Naylor AS et al. Regulation of toll-like receptor 1 and -2 in neonatal mice brains after hypoxia-ischemia. J Neuroinflammation. 2011 May 10 [PMID: 21569241]
Bansal K, Elluru SR, Narayana Y et al. PE_PGRS antigens of Mycobacterium tuberculosis induce maturation and activation of human dendritic cells. J Immunol. 2010 Apr 1 [PMID: 20176745] (WB)

Details:
WB in Fig 4B, 4C using HEk-293 cells transiently transfected with TLR2 or TLR2 dominant negative constructs: 1. TLR1 (IMG-5012), 2. TLR2 IMG-(6320A), 3. TLR4 (IMG-577), 4.TLR6 (IMG-527). Note: TLR2 was transfected validated in Fig 4B.
WB
Wu J, Meng Z, Jiang M et al. Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific. Immunology. 2010 Mar [PMID: 19922426]
Palladino MA, Savarese MA, Chapman JL et al. Localization of Toll-like receptors on epididymal epithelial cells and spermatozoa. Am J Reprod Immunol. 2008 Dec [PMID: 19032616]
Wong CK, Cheung PF, Ip WK, Lam CW. Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils. Am J Respir Cell Mol Biol. 2007 Jul [PMID: 17332440] (WB, Human)

Details:
Antibodies cited (human blood eosinophils and neutrophils from buffy coat): For WB, Fig. 1A: TLR1 (IMG-5012), TLR5 (IMG-664), TLR6 (IMG-304A), TLR7 (IMG-540), TLR8 (IMG-321A), TLR9 (IMG-305A). For Flow (Intracellular) and Flow (Surface), Fig. 1B: TLR1 (IM
WB Human
Show All 11 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Blogs on TLR1.

Toll-like receptor 2 activation contributes to oral squamous cell carcinoma development and miRNA-mediated drug resistance
By Jamshed Arslan, Pharm. D., PhD. Squamous cell carcinoma is the most common cancer in the oral cavity.1 The tumor surface biofilms in oral cancers contain high levels of aerobic and anaerobic microorganisms.1,2 Peri...  Read full blog post.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TLR1
TLR1 belongs to the Toll-like receptor (TLR) family, and is a key player in the recognition of pathogens as well as the activation of the innate immunity system. TLRs are highly conserved proteins with a high degree of structural and functional homolo...  Read full blog post.

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Bioinformatics

Gene Symbol TLR1
Entrez
Uniprot