NFkB p105/p50 Antibody (2J10D7)

Images

 
Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.
Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - analysis of human breast tumor tissue using an isotype control antibody (top) and p50 antibody (bottom) at 5 ug/ml.
Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U2-OS cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells ...read more
Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on Jurkat cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells ...read more
Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on HeLa cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC-P
Clone
2J10D7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

NFkB p105/p50 Antibody (2J10D7) Summary

Immunogen
A portion of amino acids 150-200 of human NF-kB (p50) was used as the immunogen.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NFKB1
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 2-5 ug/ml
  • Flow Cytometry
  • Immunohistochemistry-Paraffin 5 ug/ml
Control
HeLa Lysate (NB800-PC1)
NFkB p105/p50 Lysate (NBL1-13619)
Reviewed Applications
Read 1 Review rated 5
using
NB100-56583 in the following applications:

Publications
Read Publication using NB100-56583.

Reactivity Notes

Predicted to react with Mouse and Rat which are 86% homologous with the immunogen.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for NFkB p105/p50 Antibody (2J10D7)

  • DKFZp686C01211
  • DNA binding factor KBF1
  • DNA-binding factor KBF1
  • EBP-1
  • KBF1
  • NF-kappaB
  • NF-kappa-B
  • NF-kappabeta
  • NFKB-p105
  • NFKB-p50
  • nuclear factor kappa-B DNA binding subunit
  • nuclear factor NF-kappa-B p105 subunit
  • nuclear factor NF-kappa-B p50 subunit
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells 1MGC54151
  • p105
  • p50

Background

Principal Names: Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB1); DNA-binding factor KBF1; EBP-1; NFKB-p50; Official Gene Symbol: NFKB1 Gene ID: 4790 (Human) Gene Map Locus: 4q24 (human) NF-kB is a nuclear transcription factor activated by various extra and intracellular stimuli such as cytokines, UV radiation, stress, in injury, and by bacterial and viral products. It is involved in regulation of various cellular events including cell growth, differentiation, proliferation, apoptosis and inflammation. NFKB1 (p50), a 50KDa functional sub-unit of NF-kB, is a member of Rel protein family. It is synthesized as a p105 precursor protein and consists of an N-terminal conserved RHD-region containing nuclear localization signal, DNA-binding and dimerization domains. NFKB1 (p50) forms homodimers or heterodimerizes with p65, forming the functional NF-kB factor. NFKB1 (p50) directs the nuclear translocation of NF-kB and is instrumental in its DNA-bidning. Pathological role of NF-kB has been suggested in AIDS, hematogenic cancer cell metastasis and rheumatoid arthritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NFkB p105/p50 Antibody (NB100-56583)(1)

Review for NFkB p105/p50 Antibody (NB100-56583) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rabbit.

Reviews using NB100-56583:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Rabbit
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin NFkB p105/p50 NB100-56583
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reviewed by:
Anonymous
IHC-P Rabbit 10/02/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedaorta artery
SpeciesRabbit
Lot7341-1P160201

Comments

CommentsAntigen Retrieval: pressure cooker with solution Trilogy TM (Cell Marque ref: 920P-07). Antibody dilution: 0.07ug/mL (diluted 1/4000).
Secondary antibody: anti-mouse histofine ( Nisherei Bioscience Inc. ref: 414131F). Development: DAB (Dako ref: K346811-2) and counterstained with Hematoxylin.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for NFkB p105/p50 Antibody (NB100-56583) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56583AF405
Alexa Fluor 488 Labeled NB100-56583AF488
Alexa Fluor 647 Labeled NB100-56583AF647
Alexa Fluor 700 Labeled NB100-56583AF700
Biotin Labeled NB100-56583B
DyLight 350 Labeled NB100-56583UV
DyLight 405 Labeled NB100-56583V
DyLight 488 Labeled NB100-56583G
DyLight 550 Labeled NB100-56583R
DyLight 650 Labeled NB100-56583C
DyLight 680 Labeled NB100-56583FR
DyLight 755 Labeled NB100-56583IR
HRP Labeled NB100-56583H

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Bioinformatics Tool for NFkB p105/p50 Antibody (NB100-56583)

Discover related pathways, diseases and genes to NFkB p105/p50 Antibody (NB100-56583). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for NFkB p105/p50 Antibody (NB100-56583)

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PTMs for NFkB p105/p50 Antibody (NB100-56583)

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Research Areas for NFkB p105/p50 Antibody (NB100-56583)

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Blogs on NFkB p105/p50.

Nuclear Factor kappa B Signaling in the Immune System
Nuclear Factor kappa B (NFkB) binds to the kappa-beta site of the immunoglobulin kappa light chain gene enhancer. Thus NFkB has become one of the widely studied transcription factors in innate and adaptive immune responses. The NFkB family is composed...  Read full blog post.

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Recent Reviews

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1
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Anonymous
10/02/2017
Application: IHC-P
Species: Rabbit

Bioinformatics

Gene Symbol NFKB1
Entrez
Uniprot