Recombinant Human IFN-gamma Protein

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1 μg/lane of Recombinant Human IFN-gamma was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
Recombinant Human IFN-gamma (Catalog # 285‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is ...read more

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IFN-gamma Protein Summary

Details of Functionality
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 0.15-0.75 ng/mL.
The specific activity of Recombinant Human IFN-gamma is approximately 2 x 104 IU/μg, which is calibrated against human IFN-gamma Standard (NIBSC code: 87/586).
Source
E. coli-derived human IFN-gamma protein
Gln24-Gln166 with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IFNG
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
16.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17 kDa, reducing conditions
Reviewed Applications
Read 2 Reviews rated 5
using
285-IF in the following application:

Publications
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285-IF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80 with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile, deionized water.  

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IFN-gamma Protein

  • IFG
  • IFI
  • IFNG
  • IFNgamma
  • IFN-gamma
  • Immune interferon
  • interferon gamma
  • interferon, gamma

Background

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59%‑64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37%‑43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI ( alpha subunits) which then interact with IFN-gamma RII ( beta  subunits) to form the functional receptor complex of two alpha and two beta  subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).
  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Gray, P.W. and D.V. Goeddel (1982) Nature 298:859.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

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285-IF
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Applications: Bioactivity

Publications for IFN-gamma (285-IF)(302)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Hamster, N/A, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 7 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Standard), In Vivo, Western Blot, c.


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Binding Assay
(1)
Bioassay
(287)
Cell Culture
(1)
ELISA (Standard)
(6)
In Vivo
(2)
Western Blot
(1)
c
(1)
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Human
(286)
Mouse
(4)
Rat
(1)
Hamster
(1)
N/A
(6)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 302. Show All 302 Publications.
Publications using 285-IF Applications Species
CH Yu, B Suh, I Shin, EH Kim, D Kim, YJ Shin, SY Chang, SH Baek, H Kim, ON Bae Inhibitory Effects of a Novel Chrysin-Derivative, CPD 6, on Acute and Chronic Skin Inflammation Int J Mol Sci, 2019;20(11):. 2019 [PMID: 31141897] (Bioassay, Human) Bioassay Human
SM Ferrari, F Ragusa, SR Paparo, F Nasini, M Nardi, SS Franceschi, P Fallahi, A Antonelli Differential modulation of CXCL8 versus CXCL10, by cytokines, PPAR-gamma, or PPAR-alpha agonists, in primary cells from Graves&#039; disease and ophthalmopathy Autoimmun Rev, 2019;0(0):. 2019 [PMID: 31059842] (Bioassay, Human) Bioassay Human
A Ruiz, S Guzmán-Bel, LE Carreto-Bi, Y Gonzalez, E Juárez DNA from virulent M. tuberculosis induces TNF-? production and autophagy in M1 polarized macrophages Microb. Pathog., 2019;0(0):. 2019 [PMID: 31054870] (Bioassay, Human) Bioassay Human
S Vander Bek, JC de Vries, B Meier-Schi, P Meyer, D Jiang, A Sindrilaru, FF Ferreira, A Hainzl, S Schatz, J Muschhamme, NJ Scheurmann, P Kampilafko, AM Seitz, L Dürselen, A Ignatius, MA Kluth, C Ganss, M Wlaschek, K Singh, P Maity, NY Frank, MH Frank, K Scharffett Newly defined ABCB5+ dermal mesenchymal stem cells promote healing of chronic iron overload wounds via secretion of interleukin-1 receptor antagonist Stem Cells, 2019;0(0):. 2019 [PMID: 31002437] (Bioassay, Human) Bioassay Human
AT Dang, RM Teles, PT Liu, A Choi, A Legaspi, EN Sarno, MT Ochoa, K Parvatiyar, G Cheng, M Gilliet, BR Bloom, RL Modlin Autophagy links antimicrobial activity with antigen presentation in Langerhans cells JCI Insight, 2019;4(8):. 2019 [PMID: 30996142] (Bioassay, Human) Bioassay Human
JC de Quaglia, AM Della Cole, TP Gardizani, GG Romagnoli, R Kaneno, LA Dias-Melic Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis J Immunol Res, 2019;2019(0):1529189. 2019 [PMID: 30882002] (Bioassay, Human) Bioassay Human
M Ashizawa, H Okayama, T Ishigame, AK Thar Min, K Saito, D Ujiie, Y Murakami, T Kikuchi, Y Nakayama, M Noda, T Tada, H Endo, S Fujita, W Sakamoto, M Saito, Z Saze, T Momma, S Ohki, K Mimura, K Kono microRNA-148a-3p regulates immunosuppression in DNA mismatch repair-deficient colorectal cancer by targeting PD-L1 Mol. Cancer Res., 2019;0(0):. 2019 [PMID: 30872332] (Bioassay, Human) Bioassay Human
N Roper, S Gao, TK Maity, AR Banday, X Zhang, A Venugopala, CM Cultraro, R Patidar, S Sindiri, AL Brown, A Goncearenc, AR Panchenko, R Biswas, A Thomas, A Rajan, CA Carter, DE Kleiner, SM Hewitt, J Khan, L Prokunina-, U Guha APOBEC Mutagenesis and Copy-Number Alterations Are Drivers of Proteogenomic Tumor Evolution and Heterogeneity in Metastatic Thoracic Tumors Cell Rep, 2019;26(10):2651-2666.e6. 2019 [PMID: 30840888] (Bioassay, Human) Bioassay Human
M Kita, Y Ano, A Inoue, J Aoki Identification of P2Y receptors involved in oleamide-suppressing inflammatory responses in murine microglia and human dendritic cells Sci Rep, 2019;9(1):3135. 2019 [PMID: 30816271] (Bioassay, Human) Bioassay Human
F Feng, J Zhao, P Li, R Li, L Chen, C Sun Preexisting Virus-Specific T Lymphocytes-Mediated Enhancement of Adenovirus Infections to Human Blood CD14+ Cells Viruses, 2019;11(2):. 2019 [PMID: 30781810] (Bioassay, Human) Bioassay Human
Show All 302 Publications.

Reviews for IFN-gamma (285-IF) (2) 52

Average Rating: 5
(Based on 2 reviews)

Reviews using 285-IF:
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CellProlif
(1)
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 IFN-gamma 285-IF
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reviewed by:
Anonymous
06/09/2019
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Summary

LotRAX2218012

Comments

CommentsThe expression of HLA-DR increased with MDAMB231 cells incubated with 1000 IU mL–1 IFN-gamma for 72 h
Cell Proliferation IFN-gamma 285-IF
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reviewed by:
Anonymous
CellProlif 10/07/2018
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Summary

ApplicationCell Proliferation
LotRAX2218012

Comments

CommentsHT-29 Cell lines were treated with 100 U/ml IFN gamma, after which HLA-DR expression was examined by flow cytometer

FAQs for IFN-gamma (285-IF) (0)

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Blogs on IFN-gamma.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

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Anonymous
06/09/2019
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Anonymous
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Application: CellProlif
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Bioinformatics

Gene Symbol IFNG
Uniprot