Recombinant Human IFN-gamma Protein

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1 μg/lane of Recombinant Human IFN-gamma was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
Recombinant Human IFN-gamma (Catalog # 285‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price

Recombinant Human IFN-gamma Protein Summary

Details of Functionality
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 0.15-0.75 ng/mL.
The specific activity of Recombinant Human IFN-gamma is approximately 2 x 104 IU/μg, which is calibrated against human IFN-gamma Standard (NIBSC code: 87/586).
Source
E. coli-derived human IFN-gamma protein
Gln24-Gln166 with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IFNG
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
16.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17 kDa, reducing conditions
Publications
Read Publications using
285-IF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80 with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile, deionized water.  

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IFN-gamma Protein

  • IFG
  • IFI
  • IFNG
  • IFNgamma
  • IFN-gamma
  • Immune interferon
  • interferon gamma
  • interferon, gamma

Background

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59%‑64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37%‑43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI ( alpha subunits) which then interact with IFN-gamma RII ( beta  subunits) to form the functional receptor complex of two alpha and two beta  subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).
  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Gray, P.W. and D.V. Goeddel (1982) Nature 298:859.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

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285-IF
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Applications: Bioactivity

Publications for IFN-gamma (285-IF)(260)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Hamster, N/A, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 7 applications: Binding Assay, Bioassay, ELISA Standard, In Vivo, WB Ctrl, c, cell culture.


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Binding Assay
(1)
Bioassay
(246)
ELISA Standard
(6)
In Vivo
(2)
WB Ctrl
(1)
c
(1)
cell culture
(1)
All Applications
Filter By Species
Human
(245)
Mouse
(3)
Rat
(1)
Hamster
(1)
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(6)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 260. Show All 260 Publications.
Publications using 285-IF Applications Species
G Genard, AC Wera, C Huart, B Le Calve, S Penninckx, A Fattacciol, T Tabarrant, C Demazy, N Ninane, AC Heuskin, S Lucas, C Michiels Proton irradiation orchestrates macrophage reprogramming through NF?B signaling Cell Death Dis, 2018;9(7):728. 2018 [PMID: 29950610] (Bioassay, Human) Bioassay Human
S Nakagawa, S Serada, R Kakubari, K Hiramatsu, T Sugase, S Matsuzaki, S Matsuzaki, Y Ueda, K Yoshino, T Ohkawara, M Fujimoto, T Kishimoto, T Kimura, T Naka Intratumoral delivery of an adenoviral vector carrying the SOCS-1 gene enhances T cell-mediated anti-tumor immunity by suppressing PD-L1 Mol. Cancer Ther., 2018;0(0):. 2018 [PMID: 29891489] (Bioassay, Human) Bioassay Human
JA Marwick, R Mills, O Kay, K Michail, J Stephen, AG Rossi, I Dransfield, N Hirani Neutrophils induce macrophage anti-inflammatory reprogramming by suppressing NF-?B activation Cell Death Dis, 2018;9(6):665. 2018 [PMID: 29867198] (Bioassay, Human) Bioassay Human
KC Osum, AL Burrack, T Martinov, NL Sahli, JS Mitchell, CG Tucker, KE Pauken, K Papas, B Appakalai, JA Spanier, BT Fife Interferon-gamma drives programmed death-ligand 1 expression on islet ? cells to limit T cell function during autoimmune diabetes Sci Rep, 2018;8(1):8295. 2018 [PMID: 29844327] (Bioassay, Human) Bioassay Human
LCD Smyth, J Rustenhove, TI Park, P Schweder, D Jansson, PA Heppner, SJ O'Carroll, EW Mee, RLM Faull, M Curtis, M Dragunow Unique and shared inflammatory profiles of human brain endothelia and pericytes J Neuroinflammation, 2018;15(1):138. 2018 [PMID: 29751771] (Bioassay, Human) Bioassay Human
E Vorkapic, A Kunath, D Wågsäter Effects of osteoprotegerin/TNFRSF11B in two models of abdominal aortic aneurysms Mol Med Rep, 2018;0(0):. 2018 [PMID: 29749489] (Bioassay, Human) Bioassay Human
W Vermi, A Micheletti, G Finotti, C Tecchio, F Calzetti, S Costa, M Bugatti, S Calza, C Agostinell, S Pileri, P Balzarini, A Tucci, G Rossi, L Furlani, G Todeschini, A Zamò, F Facchetti, L Lorenzi, S Lonardi, MA Cassatella slan+ monocytes and macrophages mediate CD20-dependent B cell lymphoma elimination via ADCC and ADCP Cancer Res., 2018;0(0):. 2018 [PMID: 29748373] (Bioassay, Human) Bioassay Human
AE Dodi, IO Ajayi, C Chang, M Beard, SL Ashley, SK Huang, VJ Thannickal, DJ Tschumperl, TH Sisson, JC Horowitz Regulation of fibroblast Fas expression by soluble and mechanical pro-fibrotic stimuli Respir. Res., 2018;19(1):91. 2018 [PMID: 29747634] (Bioassay, Human) Bioassay Human
S McGarrity, Ó Anuforo, H Halldórsso, A Bergmann, S Halldórsso, S Palsson, HH Henriksen, PI Johansson, Ó Rolfsson Metabolic systems analysis of LPS induced endothelial dysfunction applied to sepsis patient stratification Sci Rep, 2018;8(1):6811. 2018 [PMID: 29717213] (Bioassay, Human) Bioassay Human
RB Nahomi, S Sampathkum, AM Myers, L Elghazi, DG Smith, J Tang, CA Lee, TS Kern, RH Nagaraj, PE Fort The Absence of Indoleamine 2,3-Dioxygenase Inhibits Retinal Capillary Degeneration in Diabetic Mice Invest. Ophthalmol. Vis. Sci., 2018;59(5):2042-2053. 2018 [PMID: 29677366] (Bioassay, Human) Bioassay Human
Show All 260 Publications.

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Bioinformatics

Gene Symbol IFNG
Entrez
Uniprot