Recombinant Human IFN-gamma Protein


Recombinant Human IFN-gamma (Catalog # 285-IF) has a molecular weight (MW) of 34.9 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer.  MW may differ from predicted MW due to post-translational more
1 μg/lane of Recombinant Human IFN-gamma was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
Recombinant Human IFN-gamma (Catalog # 285‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The activity is over 2-fold greater more

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Recombinant Human IFN-gamma Protein Summary

Additional Information
Analyzed by SEC-MALS
Details of Functionality
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 0.15-0.75 ng/mL.
E. coli-derived human IFN-gamma protein
Gln24-Gln166 with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
16.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
17 kDa, reducing conditions
Reviewed Applications
Read 2 Reviews rated 5
285-IF in the following application:

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285-IF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80 with BSA as a carrier protein.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile, deionized water.  


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IFN-gamma Protein

  • IFG
  • IFI
  • IFNG
  • IFNgamma
  • IFN-gamma
  • Immune interferon
  • interferon gamma
  • interferon, gamma


Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma , 59%‑64% with bovine, canine, equine, feline, and porcine IFN‑ gamma , and 37%‑43% with cotton rat, mouse, and rat IFN-gamma . IFN-gamma dimers bind to IFN-gamma RI ( alpha subunits) which then interact with IFN-gamma RII ( beta  subunits) to form the functional receptor complex of two alpha and two beta  subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).
  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Gray, P.W. and D.V. Goeddel (1982) Nature 298:859.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

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Publications for IFN-gamma (285-IF)(427)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Hamster, Porcine, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 11 applications: Binding Assay, Bioassay, Cell Culture, Differentiation, ELISA (Capture), ELISA (Standard), ELISA Standard, In Vivo, Reference Standard, Stimulation, Western Blot.

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Primate - Chlorocebus pygerythrus (Vervet Monkey)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
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Showing Publications 1 - 10 of 427. Show All 427 Publications.
Publications using 285-IF Applications Species
Doshi, A;Erickson, P;Teryek, M;Parekkadan, B; Dynamics of Ex Vivo Mesenchymal Stromal Cell Potency under Continuous Perfusion International journal of molecular sciences 2023-05-31 [PMID: 37298556] (Bioassay, Human) Bioassay Human
Soldati, S;B�r, A;Vladymyrov, M;Glavin, D;McGrath, JL;Gosselet, F;Nishihara, H;Goelz, S;Engelhardt, B; High levels of endothelial ICAM-1 prohibit natalizumab mediated abrogation of CD4+ T cell arrest on the inflamed BBB under flow in vitro Journal of neuroinflammation 2023-05-23 [PMID: 37221552] (Bioassay, Human) Bioassay Human
Zheng, X;Sarode, P;Weigert, A;Turkowski, K;Chelladurai, P;G�nther, S;Kuenne, C;Winter, H;Stenzinger, A;Reu, S;Grimminger, F;Stiewe, T;Seeger, W;Pullamsetti, SS;Savai, R; The HDAC2-SP1 axis orchestrates pro-tumor macrophage polarization Cancer research 2023-05-19 [PMID: 37205635] (Bioassay, Human) Bioassay Human
CY Lin, KY Huang, SH Kao, MS Lin, CC Lin, SC Yang, WC Chung, YH Chang, RJ Chein, PC Yang Small-molecule PIK-93 modulates the tumor microenvironment to improve immune checkpoint blockade response Science Advances, 2023-04-07;9(14):eade9944. 2023-04-07 [PMID: 37027467] (Bioassay, Human) Bioassay Human
D Avancini, A Testori, L Fresolone, G Andolfi, M Vuono, V Martinelli, FR Santoni de, S Gregori Aryl hydrocarbon receptor activity downstream of IL-10 signaling is required to promote regulatory functions in human dendritic cells Cell Reports, 2023-03-03;42(3):112193. 2023-03-03 [PMID: 36870061] (Reference Standard, Human) Reference Standard Human
H Asashima, S Mohanty, M Comi, WE Ruff, KB Hoehn, P Wong, J Klein, C Lucas, I Cohen, S Coffey, N Lele, L Greta, K Raddassi, O Chaudhary, A Unterman, B Emu, SH Kleinstein, RR Montgomery, A Iwasaki, CS Dela Cruz, N Kaminski, AC Shaw, DA Hafler, TS Sumida PD-1highCXCR5-CD4+ peripheral helper T�cells promote CXCR3+ plasmablasts in human acute viral infection Cell Reports, 2023-01-02;0(0):111895. 2023-01-02 [PMID: 36596303] (Cell Culture, Human) Cell Culture Human
E Turner, L Chen, JG Foulke, Z Gu, F Tian CRISPR/Cas9 Edited RAS &amp; MEK Mutant Cells Acquire BRAF and MEK Inhibitor Resistance with MEK1 Q56P Restoring Sensitivity to MEK/BRAF Inhibitor Combo and KRAS G13D Gaining Sensitivity to Immunotherapy Cancers, 2022-11-05;14(21):. 2022-11-05 [PMID: 36358868] (Bioassay, Human) Bioassay Human
S Hayashi, CK Muraleedha, M Oku, S Tomar, SP Hogan, M Quiros, CA Parkos, A Nusrat Intestinal epithelial BLT1 promotes mucosal repair JCI Insight, 2022-12-08;0(0):. 2022-12-08 [PMID: 36301666] (Bioassay, Human) Bioassay Human
P Pavlidis, A Tsakmaki, A Treveil, K Li, D Cozzetto, F Yang, U Niazi, BH Hayee, M Saqi, J Friedman, T Korcsmaros, G Bewick, N Powell Cytokine responsive networks in human colonic epithelial organoids unveil a molecular classification of inflammatory bowel disease Cell Reports, 2022-09-27;40(13):111439. 2022-09-27 [PMID: 36170836] (Bioassay, Human) Bioassay Human
S Mitra, R Gera, J Sundheimer, M Lemee, LU Wahlberg, B Linderoth, M Eriksdotte, H Behbahani Microglia Impairs Proliferation and Induces Senescence In-Vitro in NGF Releasing Cells Used in Encapsulated Cell Biodelivery for Alzheimer&#039;s Disease Therapy International Journal of Molecular Sciences, 2022-08-12;23(16):. 2022-08-12 [PMID: 36012296] (Bioassay, Human) Bioassay Human
Show All 427 Publications.

Reviews for IFN-gamma (285-IF) (2) 52

Average Rating: 5
(Based on 2 reviews)

Reviews using 285-IF:
Filter by Applications
In vitro bioactivity in cell culture
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In vitro bioactivity in cell culture IFN-gamma 285-IF
reviewed by:
Verified Customer
In vitro bioactivity in cell culture 06/09/2019


ApplicationIn vitro bioactivity in cell culture


CommentsThe expression of HLA-DR increased with MDAMB231 cells incubated with 1000 IU mL–1 IFN-gamma for 72 h
Cell Proliferation IFN-gamma 285-IF
reviewed by:
Verified Customer
CellProlif 10/07/2018


ApplicationCell Proliferation


CommentsHT-29 Cell lines were treated with 100 U/ml IFN gamma , after which HLA-DR expression was examined by flow cytometer

FAQs for IFN-gamma (285-IF). (Showing 1 - 1 of 1 FAQs).

  1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
    • We are research use only.

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Blogs on IFN-gamma.

Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ...  Read full blog post.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.

  Read full blog post.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

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Application: In vitro bioactivity in cell culture

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Gene Symbol IFNG