Recombinant Human IFN-gamma Protein

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Recombinant Human IFN-gamma (Catalog # 285‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The activity is over 2-fold greater ...read more
1 μg/lane of Recombinant Human IFN-gamma was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IFN-gamma Protein Summary

Details of Functionality
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 0.15-0.75 ng/mL.
The specific activity of Recombinant Human IFN-gamma is approximately 2 x 104 IU/μg, which is calibrated against human IFN-gamma Standard (NIBSC code: 87/586).   Specific activity is for reference purposes only and is not routinely tested.  
Source
E. coli-derived human IFN-gamma protein
Gln24-Gln166 with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IFNG
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
16.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17 kDa, reducing conditions
Reviewed Applications
Read 2 Reviews rated 5
using
285-IF in the following application:

Publications
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285-IF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80 with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile, deionized water.  

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IFN-gamma Protein

  • IFG
  • IFI
  • IFNG
  • IFNgamma
  • IFN-gamma
  • Immune interferon
  • interferon gamma
  • interferon, gamma

Background

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma , 59%‑64% with bovine, canine, equine, feline, and porcine IFN‑ gamma , and 37%‑43% with cotton rat, mouse, and rat IFN-gamma . IFN-gamma dimers bind to IFN-gamma RI ( alpha subunits) which then interact with IFN-gamma RII ( beta  subunits) to form the functional receptor complex of two alpha and two beta  subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).
  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Gray, P.W. and D.V. Goeddel (1982) Nature 298:859.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

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Publications for IFN-gamma (285-IF)(363)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Hamster, N/A, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 10 applications: Binding Assay, Bioassay, Cell Culture, Differentiation, ELISA (Capture), ELISA (Standard), ELISA Standard, In Vivo, Western Blot, c.


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Binding Assay
(1)
Bioassay
(319)
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(26)
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(1)
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(6)
ELISA Standard
(1)
In Vivo
(2)
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(1)
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Human
(345)
Mouse
(6)
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Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 363. Show All 363 Publications.
Publications using 285-IF Applications Species
S Nakajima, K Mimura, T Matsumoto, AK Thar Min, M Ito, H Nakano, P Neupane, Y Kanke, H Okayama, M Saito, T Momma, Y Watanabe, H Hanayama, S Hayase, Z Saze, K Kono The effects of T-DXd on the expression of HLA class I and chemokines CXCL9/10/11 in HER2-overexpressing gastric cancer cells Scientific Reports, 2021;11(1):16891. 2021 [PMID: 34413454] (Bioassay, Human) Bioassay Human
C Prelli Boz, R Nchioua, M Volcic, L Koepke, J Krüger, D Schütz, S Heller, CM Stürzel, D Kmiec, C Conzelmann, J Müller, F Zech, E Braun, R Gro beta , L Wettstein, T Weil, J Wei beta , F Diofano, AA Rodríguez, S Wiese, D Sauter, J Münch, C Goffinet, A Catanese, M Schön, TM Boeckers, S Stenger, K Sato, S Just, A Kleger, KMJ Sparrer, F Kirchhoff IFITM proteins promote SARS-CoV-2 infection and are targets for virus inhibition in vitro Nature Communications, 2021;12(1):4584. 2021 [PMID: 34321474] (Bioassay, Human) Bioassay Human
S Luostarine, M Hämäläinen, N Hatano, K Muraki, E Moilanen The inflammatory regulation of TRPA1 expression in human A549 lung epithelial cells Pulmonary pharmacology & therapeutics, 2021;70(0):102059. 2021 [PMID: 34302984] (Bioassay, Human) Bioassay Human
D Lobato-Már, J Xu, GÖ Güler, A Ojiakor, M Pilhofer, S Mostowy Mechanistic insight into bacterial entrapment by septin cage reconstitution Nature Communications, 2021;12(1):4511. 2021 [PMID: 34301939] (Bioassay, Human) Bioassay Human
R Luna-Yolba, J Marmoiton, V Gigo, X Marechal, E Boet, A Sahal, N Alet, I Abramovich, E Gottlieb, V Visentin, MR Paillasse, JE Sarry Disrupting Mitochondrial Electron Transfer Chain Complex I Decreases Immune Checkpoints in Murine and Human Acute Myeloid Leukemic Cells Cancers, 2021;13(14):. 2021 [PMID: 34298712] (Bioassay, Human) Bioassay Human
X Yang, X Zhao, Y Zhu, Y Shen, Y Wang, P Lu, Z Jiang, H Pan, J Yang, J Xun, L Zhao, J Wang, Z Liang, X Shen, Y Liang, Q Lin, H Liang, L Jin, D Zhang, J Liu, B Wang, S Jiang, J Xu, H Wu, H Lu, H Zhu FKBP3 Induces Human Immunodeficiency Virus Type 1 Latency by Recruiting Histone Deacetylase 1/2 to the Viral Long Terminal Repeat MBio, 2021;0(0):e0079521. 2021 [PMID: 34281390] (Bioassay, Human) Bioassay Human
JS Aguilar Dí, HL Glenn, M Knappenber, CR Borges Oxidized-Desialylated Low-Density Lipoprotein Inhibits the Antitumor Functions of Lymphokine Activated Killer Cells Journal of Cancer, 2021;12(16):4993-5004. 2021 [PMID: 34234868] (ELISA (Capture), Human) ELISA (Capture) Human
AC Tecalco-Cr, M Macías-Sil, JO Ramírez-Ja, B Méndez-Amb Identification of genes modulated by interferon gamma in breast cancer cells Biochemistry and Biophysics Reports, 2021;27(0):101053. 2021 [PMID: 34189281] (Cell Culture, Human) Cell Culture Human
SP Totten, YK Im, E Cepeda Cañ, O Najyb, A Nguyen, S Hébert, R Ahn, K Lewis, B Lebeau, R La Selva, V Sabourin, C Martínez, P Savage, H Kuasne, D Avizonis, N Santos Mar, C Chabot, A Aguilar-Ma, ML Goulet, M Dankner, M Witcher, K Petrecca, M Basik, M Pollak, I Topisirovi, R Lin, PM Siegel, CL Kleinman, M Park, J St-Pierre, J Ursini-Sie STAT1 potentiates oxidative stress revealing a targetable vulnerability that increases phenformin efficacy in breast cancer Nature Communications, 2021;12(1):3299. 2021 [PMID: 34083537] (Bioassay, Human) Bioassay Human
MC Suárez-Arr, A Méndez-Ten, V Pérez-Kold, EM Fuentes-Pa Claudin-Low Breast Cancer Inflammatory Signatures Support Polarization of M1-Like Macrophages with Protumoral Activity Cancers, 2021;13(9):. 2021 [PMID: 34067089] (Bioassay, Human) Bioassay Human
Show All 363 Publications.

Reviews for IFN-gamma (285-IF) (2) 52

Average Rating: 5
(Based on 2 reviews)

Reviews using 285-IF:
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 IFN-gamma 285-IF
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5
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06/09/2019
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Summary

LotRAX2218012

Comments

CommentsThe expression of HLA-DR increased with MDAMB231 cells incubated with 1000 IU mL–1 IFN-gamma for 72 h
Cell Proliferation IFN-gamma 285-IF
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5
reviewed by:
Verified Customer
CellProlif 10/07/2018
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Summary

ApplicationCell Proliferation
LotRAX2218012

Comments

CommentsHT-29 Cell lines were treated with 100 U/ml IFN gamma , after which HLA-DR expression was examined by flow cytometer

FAQs for IFN-gamma (285-IF). (Showing 1 - 1 of 1 FAQs).

  1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
    • We are research use only.

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Blogs on IFN-gamma.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

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