Recombinant Human IFN-gamma Protein

Images

 
Recombinant Human IFN-gamma (Catalog # 285‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The activity is over 2-fold greater ...read more
1 μg/lane of Recombinant Human IFN-gamma was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human IFN-gamma Protein Summary

Details of Functionality
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 0.15-0.75 ng/mL.
The specific activity of Recombinant Human IFN-gamma is approximately 2 x 104 IU/μg, which is calibrated against human IFN-gamma Standard (NIBSC code: 87/586).
Source
E. coli-derived human IFN-gamma protein
Gln24-Gln166 with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IFNG
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
16.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17 kDa, reducing conditions
Reviewed Applications
Read 2 Reviews rated 5
using
285-IF in the following application:

Publications
Read Publications using
285-IF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80 with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile, deionized water.  

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IFN-gamma Protein

  • IFG
  • IFI
  • IFNG
  • IFNgamma
  • IFN-gamma
  • Immune interferon
  • interferon gamma
  • interferon, gamma

Background

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59%‑64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37%‑43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI ( alpha subunits) which then interact with IFN-gamma RII ( beta  subunits) to form the functional receptor complex of two alpha and two beta  subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).
  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Gray, P.W. and D.V. Goeddel (1982) Nature 298:859.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

Customers Who Viewed This Item Also Viewed...

NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
NBP2-75930
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB300-605
Species: Hu, Mu, Rt, Po
Applications: WB, Flow, IB, ICC/IF, IHC, IHC-P, In vitro
NBP2-45041
Species: Hu, Po
Applications: Flow, IHC, IHC-Fr, IF
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
285-IF
Species: Hu
Applications: Bioactivity

Publications for IFN-gamma (285-IF)(339)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Hamster, N/A, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 9 applications: Binding Assay, Bioassay, Cell Culture, Differentiation, ELISA (Standard), ELISA Standard, In Vivo, Western Blot, c.


Filter By Application
Binding Assay
(1)
Bioassay
(304)
Cell Culture
(18)
Differentiation
(2)
ELISA (Standard)
(6)
ELISA Standard
(1)
In Vivo
(2)
Western Blot
(1)
c
(1)
All Applications
Filter By Species
Human
(322)
Mouse
(5)
Rat
(1)
Hamster
(1)
N/A
(6)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 339. Show All 339 Publications.
Publications using 285-IF Applications Species
NC Leite, E Sintov, TB Meissner, MA Brehm, DL Greiner, DM Harlan, DA Melton Modeling Type 1 Diabetes In�Vitro Using Human Pluripotent Stem Cells Cell Rep, 2020;32(2):107894. 2020 [PMID: 32668238] (Bioassay, Human) Bioassay Human
MY Hsieh, JJ Lin, SH Hsia, JL Huang, KW Yeh, KW Chang, WI Lee Diminished toll-like receptor response in febrile infection-related epilepsy syndrome (FIRES) Biomed J, 2020;0(0):. 2020 [PMID: 32651134] (Bioassay, Human) Bioassay Human
H El-Kehdy, M Najar, J De Kock, DM Agha, V Rogiers, M Merimi, L Lagneaux, EM Sokal, M Najimi Inflammation Differentially Modulates the Biological Features of Adult Derived Human Liver Stem/Progenitor Cells Cells, 2020;9(7):. 2020 [PMID: 32650454] (Cell Culture, Human) Cell Culture Human
C Su, ME Johnson, A Torres, RM Thomas, E Manduchi, P Sharma, P Mehra, C Le Coz, ME Leonard, S Lu, KM Hodge, A Chesi, J Pippin, N Romberg, SFA Grant, AD Wells Mapping effector genes at lupus GWAS loci using promoter Capture-C in follicular helper T cells Nat Commun, 2020;11(1):3294. 2020 [PMID: 32620744] (Cell Culture, Human) Cell Culture Human
K Shan, N Feng, J Cui, S Wang, H Qu, G Fu, J Li, H Chen, X Wang, R Wang, Y Qi, Z Gu, YQ Chen Resolvin D1 and D2 inhibit tumour growth and inflammation via modulating macrophage polarization J. Cell. Mol. Med., 2020;0(0):. 2020 [PMID: 32469149] (Bioassay, Human) Bioassay Human
J Liu, S Xavy, S Mihardja, S Chen, K Sompalli, D Feng, TS Choi, B Agoram, R Majeti, IL Weissman, JP Volkmer Targeting macrophage checkpoint inhibitor SIRPa for anticancer therapy JCI Insight, 2020;0(0):. 2020 [PMID: 32427583] (Bioassay, Human) Bioassay Human
YD Kim, SM Park, HC Ha, AR Lee, H Won, H Cha, S Cho, JM Cho HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment J Cancer, 2020;11(14):4059-4072. 2020 [PMID: 32368288] (Bioassay, Human) Bioassay Human
H Ray-Jones, K Duffus, A McGovern, P Martin, C Shi, J Hankinson, O Gough, A Yarwood, AP Morris, A Adamson, C Taylor, J Ding, VP Gaddi, Y Fu, P Gaffney, G Orozco, RB Warren, S Eyre Mapping DNA interaction landscapes in psoriasis susceptibility loci highlights KLF4 as a target gene in 9q31 BMC Biol., 2020;18(1):47. 2020 [PMID: 32366252] (Cell Culture, Human) Cell Culture Human
MN Dehno, Y Li, H Weiher, IGH Schmidt-Wo Increase in Efficacy of Checkpoint Inhibition by Cytokine-Induced-Killer Cells as a Combination Immunotherapy for Renal Cancer Int J Mol Sci, 2020;21(9):. 2020 [PMID: 32349280] (Cell Culture, Human) Cell Culture Human
AM Kell, EA Hemann, JB Turnbull, M Gale RIG-I-like receptor activation drives type I IFN and antiviral signaling to limit�Hantaan orthohantavirus replication PLoS Pathog., 2020;16(4):e1008483. 2020 [PMID: 32330200] (Cell Culture, Human) Cell Culture Human
Show All 339 Publications.

Reviews for IFN-gamma (285-IF) (2) 52

Average Rating: 5
(Based on 2 reviews)

Reviews using 285-IF:
Filter by Applications
CellProlif
(1)
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
 IFN-gamma 285-IF
Enlarge
5
reviewed by:
Anonymous
06/09/2019
View

Summary

LotRAX2218012

Comments

CommentsThe expression of HLA-DR increased with MDAMB231 cells incubated with 1000 IU mL–1 IFN-gamma for 72 h
Cell Proliferation IFN-gamma 285-IF
Enlarge
5
reviewed by:
Anonymous
CellProlif 10/07/2018
View

Summary

ApplicationCell Proliferation
LotRAX2218012

Comments

CommentsHT-29 Cell lines were treated with 100 U/ml IFN gamma, after which HLA-DR expression was examined by flow cytometer

FAQs for IFN-gamma (285-IF). (Showing 1 - 1 of 1 FAQs).

  1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
    • We are research use only.

Other Available Formats

Additional IFN-gamma Products

Bioinformatics Tool for IFN-gamma (285-IF)

Discover related pathways, diseases and genes to IFN-gamma (285-IF). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IFN-gamma (285-IF)

Discover more about diseases related to IFN-gamma (285-IF).
 

Pathways for IFN-gamma (285-IF)

View related products by pathway.

PTMs for IFN-gamma (285-IF)

Learn more about PTMs related to IFN-gamma (285-IF).

Blogs on IFN-gamma.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
06/09/2019
Application:
Species:

Anonymous
10/07/2018
Application: CellProlif
Species:

Bioinformatics

Gene Symbol IFNG
Uniprot