Recombinant Human IFN-gamma Protein

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1 μg/lane of Recombinant Human IFN-gamma was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
Recombinant Human IFN-gamma (Catalog # 285‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IFN-gamma Protein Summary

Details of Functionality
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED 50 for this effect is 0.15-0.75 ng/mL. The specific activity of Recombinant Human IFN-gamma is approximately 2 x 10 4 IU/μg, which is calibrated against human IFN-gamma Standard (NIBSC code: 87/586).
Source
E. coli-derived human IFN-gamma protein Gln24-Gln166 with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IFNG
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
16.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17 kDa, reducing conditions
Publications
Read Publications using
285-IF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80 with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile, deionized water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IFN-gamma Protein

  • IFG
  • IFI
  • IFNG
  • IFNgamma
  • IFN-gamma
  • Immune interferon
  • interferon gamma
  • interferon, gamma

Background

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59%-64% with bovine, canine, equine, feline, and porcine IFN-gamma, and 37%-43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI ( alpha subunits) which then interact with IFN-gamma RII ( beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up-regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).
  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Gray, P.W. and D.V. Goeddel (1982) Nature 298:859.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

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Publications for IFN-gamma (285-IF)(255)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Hamster, N/A, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 7 applications: Binding Assay, Bioassay, ELISA Standard, In Vivo, WB Ctrl, c, cell culture.


Filter By Application
Binding Assay
(1)
Bioassay
(241)
ELISA Standard
(6)
In Vivo
(2)
WB Ctrl
(1)
c
(1)
cell culture
(1)
All Applications
Filter By Species
Human
(240)
Mouse
(3)
Rat
(1)
Hamster
(1)
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(6)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 255. Show All 255 Publications.
Publications using 285-IF Applications Species
KC Osum, AL Burrack, T Martinov, NL Sahli, JS Mitchell, CG Tucker, KE Pauken, K Papas, B Appakalai, JA Spanier, BT Fife Interferon-gamma drives programmed death-ligand 1 expression on islet ? cells to limit T cell function during autoimmune diabetes Sci Rep, 2018;8(1):8295. 2018 [PMID: 29844327] (Bioassay, Human) Bioassay Human
LCD Smyth, J Rustenhove, TI Park, P Schweder, D Jansson, PA Heppner, SJ O'Carroll, EW Mee, RLM Faull, M Curtis, M Dragunow Unique and shared inflammatory profiles of human brain endothelia and pericytes J Neuroinflammation, 2018;15(1):138. 2018 [PMID: 29751771] (Bioassay, Human) Bioassay Human
W Vermi, A Micheletti, G Finotti, C Tecchio, F Calzetti, S Costa, M Bugatti, S Calza, C Agostinell, S Pileri, P Balzarini, A Tucci, G Rossi, L Furlani, G Todeschini, A Zamò, F Facchetti, L Lorenzi, S Lonardi, MA Cassatella slan+ monocytes and macrophages mediate CD20-dependent B cell lymphoma elimination via ADCC and ADCP Cancer Res., 2018;0(0):. 2018 [PMID: 29748373] (Bioassay, Human) Bioassay Human
AE Dodi, IO Ajayi, C Chang, M Beard, SL Ashley, SK Huang, VJ Thannickal, DJ Tschumperl, TH Sisson, JC Horowitz Regulation of fibroblast Fas expression by soluble and mechanical pro-fibrotic stimuli Respir. Res., 2018;19(1):91. 2018 [PMID: 29747634] (Bioassay, Human) Bioassay Human
S McGarrity, Ó Anuforo, H Halldórsso, A Bergmann, S Halldórsso, S Palsson, HH Henriksen, PI Johansson, Ó Rolfsson Metabolic systems analysis of LPS induced endothelial dysfunction applied to sepsis patient stratification Sci Rep, 2018;8(1):6811. 2018 [PMID: 29717213] (Bioassay, Human) Bioassay Human
RB Nahomi, S Sampathkum, AM Myers, L Elghazi, DG Smith, J Tang, CA Lee, TS Kern, RH Nagaraj, PE Fort The Absence of Indoleamine 2,3-Dioxygenase Inhibits Retinal Capillary Degeneration in Diabetic Mice Invest. Ophthalmol. Vis. Sci., 2018;59(5):2042-2053. 2018 [PMID: 29677366] (Bioassay, Human) Bioassay Human
C Lema, RY Reins, RL Redfern High-Mobility Group Box 1 in Dry Eye Inflammation Invest. Ophthalmol. Vis. Sci., 2018;59(5):1741-1750. 2018 [PMID: 29610858] (Bioassay, Human) Bioassay Human
S Sugita, K Makabe, Y Iwasaki, S Fujii, M Takahashi Natural Killer Cell Inhibition by HLA-E Molecules on Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelial Cells Invest. Ophthalmol. Vis. Sci., 2018;59(5):1719-1731. 2018 [PMID: 29610856] (Bioassay, Human) Bioassay Human
S Nagel, C Pommerenke, C Meyer, M Kaufmann, RAF MacLeod, HG Drexler Aberrant expression of NKL homeobox gene HLX in Hodgkin lymphoma Oncotarget, 2018;9(18):14338-14353. 2018 [PMID: 29581848] (Bioassay, Human) Bioassay Human
EY Huang, JC Chang, HH Chen, CY Hsu, HC Hsu, KL Wu Carcinoembryonic antigen as a marker of radioresistance in colorectal cancer: a potential role of macrophages BMC Cancer, 2018;18(1):321. 2018 [PMID: 29580202] (Bioassay, Human) Bioassay Human
Show All 255 Publications.

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Bioinformatics

Gene Symbol IFNG
Entrez
Uniprot