TLR4 Antibody (76B357.1)

Images

 
Western Blot: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis using 2 ug/ml on (A) human intestine and 6 ug/ml on (B) mouse intestine and C) rat intestine lysate.
Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [NB100-56566] - Human skin stained with at 5 ug/ml, peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue ...read more
Flow (Intracellular): TLR4 Antibody (76B357.1) [NB100-56566] - Analysis using PE conjugate of NBP2-27149. An intracellular stain was performed on Jurkat cells with TLR4 antibody (76B357.1) NBP2-27149PE (blue) and an ...read more
Flow Cytometry: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis of formaldehyde fixed THP-1 cells (human monocytic leukemia cells) using 2 ug/10^6 cells TLR4 antibody (clone 76B357.1) with detection employing a donkey ...read more
Flow Cytometry: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-27149. TLR4 expression on monocytes from human peripheral blood: PBMC were stained, in a 2 color flow ...read more
Flow Cytometry: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis of 10^6 ThP1 cells using 5 ug. Shaded histogram shows ThP1 cells without anti-TLR4 antibody; green represents isotype control; red represents anti-TLR4 ...read more
Flow (Cell Surface): TLR4 Antibody (76B357.1) [NB100-56566] - Analysis using the FITC conjugate of NBP2-27149. Staining of cell surface TLR4 in human monocyte-derived dendritic cells using this antibody at 1:200 (2.5 ...read more
Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [NB100-56566] - Tissue section of normal human skin at 5 ug/ml. Membrane-cytoplasmic immunopositivity of TLR4 was primarily observed in the pigmented basel cells ...read more
Immunohistochemistry-Frozen: TLR4 Antibody (76B357.1) [NB100-56566] - This image is TLR4(green) and nucleus(blue) at area postrema of the adult male mouse brain, x20 magnification. Primary antibody diluted 1:500. This ...read more
Flow (Intracellular): TLR4 Antibody (76B357.1) [NB100-56566] - Flow Cytometry: TLR4 Antibody (76B357.1) - Azide Free [NBP2-27149] - An intracellular stain was performed on Jurkat cells with TLR4 antibody (76B357.1) ...read more
Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [NB100-56566] - Tissue section of normal human breast at 5 ug/ml. The ductal/acinar epithelial cells and the myoepithelial cells showed specific ...read more
Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [NB100-56566] - Tissue section of human esophageal squamous cell carcinoma (SCC) using 5 ug/ml. Membrane-cytoplasmic immunopositivity was observed in SCC cells ...read more
Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [NB100-56566] - Tissue section of malignant stromal tumor of small intestine from human at 5 ug/ml. The representative image shows a membrane-cytoplasmic staining ...read more
Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis of Rat's salivary gland tissue section at 1:100 dilution. The antibody generated a membrane-cytoplasmic staining in the tissue with ...read more
Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis of TLR4 in paraffin-embedded formalin-fixed human colon tissue using an isotype control (top) and NB100-56566 (bottom) at 5 ug/ml.
Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [NB100-56566] - Human testis tissue stained using 5 ug/ml.
Flow Cytometry: TLR4 Antibody (76B357.1) [NB100-56566] - Surface staining of mouse splenocytes using TLR4 antibody (0.5 ug/10^6 cells) and a PE-conjugated secondary this antibody. Shaded histogram represents cells ...read more
Flow Cytometry: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis of FITC conjugate of NB100-56566. Cell surface TLR4 in human monocyte-derived dendritic cells using this antibody at 1:200 (2.5 ug). Black and green open ...read more
Flow Cytometry: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis using the FITC conjugate of NBP2-27149. TLR4 expression on monocytes from human peripheral blood: PBMC were stained, in a 2 color flow test, with CD14 PE ...read more
Flow (Intracellular): TLR4 Antibody (76B357.1) [NB100-56566] - An intracellular stain was performed on Jurkat cells with TLR4 antibody (76B357.1) NBP2-27149AF647 (blue) and a matched isotype control (orange). Cells were ...read more
Flow Cytometry: TLR4 Antibody (76B357.1) [NB100-56566] - An intracellular stain was performed on Jurkat cells with TLR4 antibody (76B357.1) NBP2-27149AF488 (blue) and a matched isotype control (orange). Cells were fixed ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Bb, ChHaSpecies Glossary
Applications WB, ChIP, Flow, Flow-CS, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, CyTOF-ready, ELISA(Cap)
Clone
76B357.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

TLR4 Antibody (76B357.1) Summary

Immunogen
This antibody was developed against a portion of amino acids 100-200 of human TLR4 (NP_612564).
Predicted Species
Chinese Hamster (100%), Baboon (100%). Backed by our 100% Guarantee.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TLR4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Chromatin Immunoprecipitation 1:10-1:500
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • In vitro assay
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.5 ug
  • Flow (Cell Surface)
  • Flow (Intracellular)
Application Notes
Flow (Intracellular) use reported in literature (See Cohen et al, 2008); Immunofluorescence (See Nowicki et al, 2009); IHC-F (Nowicki et al, 2012); Flow (cell surface) use reported in scientific literature (PMID: 23796194). Use in chromatin immunoprecipitation reported in scientific literature (PMID: 21966468). Use In vitro reported in scientific literature (PMID: 26446256). This antibody is CyTOF ready.
Agonist
Antagonist
Reviewed Applications
Read 5 Reviews rated 4.2
using
NB100-56566 in the following applications:

Publications
Read Publications using
NB100-56566 in the following applications:

Reactivity Notes

Ground squirrel reactivity reported by a customer review. Mammal reactivity reported in scientific literature (PMID: 25130694). Porcine reactivity reported in scientific literature (PMID: 29941006). Bovine reactivity reported in scientific literature (PMID: 19528595). Sheep (85%), Cat, Equine (78%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TLR4 Antibody (76B357.1)

  • 76B357.1
  • ARMD10
  • CD284 antigen
  • CD284
  • hTollhomolog of Drosophila toll
  • tlr4 76B357.1
  • TLR4 facs
  • TLR4 flow cytometry
  • TLR4 human
  • TLR4 ihc
  • TLR4 western blot
  • TLR4
  • TOLL
  • toll-like receptor 4

Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling. The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-56566
Species: Hu, Mu, Rt, Po, Bv, Bb, ChHa
Applications: WB, ChIP, Flow, Flow-CS, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, CyTOF-ready, ELISA(Cap)
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Publications for TLR4 Antibody (NB100-56566)(80)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bovine, Mammal, Porcine.

We have publications tested in 15 applications: B/N, ChIP, DB, ELISA, FLOW, Flow, Flow-CS, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, In-vitro, WB, WB,ICC/IF.


Filter By Application
B/N
(1)
ChIP
(1)
DB
(1)
ELISA
(1)
FLOW
(4)
Flow
(3)
Flow-CS
(7)
Flow-IC
(1)
ICC/IF
(6)
IHC
(4)
IHC-Fr
(1)
IHC-P
(8)
In-vitro
(1)
WB
(23)
WB,ICC/IF
(1)
All Applications
Filter By Species
Human
(19)
Mouse
(15)
Rat
(17)
Bovine
(1)
Mammal
(1)
Porcine
(3)
All Species
Showing Publications 1 - 10 of 80. Show All 80 Publications.
Publications using NB100-56566 Applications Species
Lee JY, Joo B, Nam JH et al. An Aqueous Extract of Herbal Medicine ALWPs Enhances Cognitive Performance and Inhibits LPS-Induced Neuroinflammation via FAk/NF-kB Signaling Pathways. Front Aging Neurosci. Sep 26 2018 [PMID: 30319390] (WB, Mouse) WB Mouse
Mukherjee P, Hough G, Chattopadhyay A et al. Role of Enterocyte Stearoyl Co-A Desaturase-1 in LDLR Null Mice J. Lipid Res. Aug 23 2018 [PMID: 30139760] (ELISA, Mouse) ELISA Mouse
Choi CH, Kang TH, Song JS et al. Elevated expression of pancreatic adenocarcinoma upregulated factor (PAUF) is associated with poor prognosis and chemoresistance in epithelial ovarian cancer Sci Rep Aug 15 2018 [PMID: 30111860] (IHC, Human) IHC Human
Bidne kL, kvidera SS, Ross JW et al. Impact of repeated lipopolysaccharide administration on ovarian signaling during the follicular phase of the estrous cycle in post-pubertal pigs. J. Anim. Sci. Jul 3 2018 [PMID: 29982469] (WB, Porcine) WB Porcine
Bernardini C, Zannoni A, Bertocchi M et al. Water/ethanol extract of Cucumis sativus L. fruit attenuates lipopolysaccharide-induced inflammatory response in endothelial cells. BMC Complement Altern Med. Jun 25 2018 [PMID: 29941006] (WB, Porcine) WB Porcine
Sun L, Li M, Ma X et al. Inhibiting high mobility group box-1 reduces early spinal cord edema and attenuates astrocyte activation and aquaporin-4 expression after spinal cord injury in rats J. Neurotrauma Jun 21 2018 [PMID: 29929431] (WB, Rat) WB Rat
Domon Hisanori, Nagai Kosuke, Maekawa Tomoki et al. Neutrophil Elastase Subverts the Immune Response by Cleaving Toll-Like Receptors and Cytokines in Pneumococcal Pneumonia. Frontiers in Immunology 2018 [PMID: 29922273] (WB, Mouse) WB Mouse
Bhattacharjee P, Keyel PA. Cholesterol-dependent cytolysins impair pro-inflammatory macrophage responses. Sci Rep Apr 24 2018 [PMID: 29691463] (Mouse) Mouse
Dickson MJ, Hager CL, Al-Shaibi A et al. Impact of heat stress during the follicular phase on porcine ovarian steroidogenic and phosphatidylinositol-3 signaling. J Anim Sci 2018 Jun 4 [PMID: 29684161]
Chen X, Yan X, Guo L. Inhibitory effect of Patrinia on BRL-3A cell apoptosis through the TLR4/PI3K/AKT/GSK3b and TLR4/P38/JNK signaling pathways. Mol Med Rep. 2018 Jan 22 [PMID: 29363726] (Rat) Rat
Show All 80 Publications.

Reviews for TLR4 Antibody (NB100-56566) (5) 4.25

Average Rating: 4.2
(Based on 5 reviews)
We have 5 reviews tested in 2 species: Mouse, Other.

Reviews using NB100-56566:
Filter by Applications
IHC
(1)
WB
(3)
All Applications
Filter by Species
Mouse
(3)
Other
(2)
All Species
Images Ratings Applications Species Date Details
 TLR4 NB100-56566
Enlarge
reviewed by:
Anonymous
Mouse 05/25/2017
View

Summary

Sample Testedbrain tissue slice(30um
SpeciesMouse

Comments

CommentsIHC-frozen, 1:500
Immunohistochemistry TLR4 NB100-56566
Enlarge
reviewed by:
Hayk Simonyan
IHC Mouse 05/16/2017
View

Summary

ApplicationImmunohistochemistry
Sample Tested Mouse brain,Adult mouse brain tissue,Mouse cortex
SpeciesMouse
Western Blot TLR4 NB100-56566
Enlarge
reviewed by:
Aileen Keating
WB Other 05/12/2016
View

Summary

ApplicationWestern Blot
Sample TestedPig whole ovarian lysate
SpeciesOther
LotCTJZUO

Blocking

Blocking Details5% BSA in.2% PBST for 3 hours at room temperature, rocking

Primary Anitbody

Dilution Ratio1:500 in 5% BSA.2% PBST, overnight, at 4 degrees Celsius, rocking

Secondary Antibody

Secondary DescriptionHorse anti-mouse IgG, HRP
Secondary Manufacturer Cat#Cell Signaling: 7076
Secondary Concentration1:1000 in 5% BSA.2% PBST

Details

Detection NotesECL substrate, Alpha Innotech MultiImage Light Cabinet, 30 sec exposure. Controls negative for signal (1° only, 2° only, IgG).
Western Blot TLR4 NB100-56566
Enlarge
reviewed by:
Liyong Zhang
WB Mouse 08/07/2014
View

Summary

ApplicationWestern Blot
SpeciesMouse
FileView PDF
Western Blot TLR4 NB100-56566
Enlarge
reviewed by:
Courtney Kurtz
WB Other 08/11/2011
View

Summary

ApplicationWestern Blot
Sample Testedsmall intestinal lysate, Sample Amount: 50 ug
SpeciesOther
CommentsWorks very well with chemiluminescent detection, but not quite strong enough for a good signal with colorimetric detection. Overall good! Appears slightly smaller than normal, although this can happen in my particular species. Only band observed on blot.

Blocking

Blocking DetailsBlocking Buffer: 4% milk, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1:250, Incubation Dilution Buffer: 4% milk, Incubation Time: overnight, Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: goat anti-mouse poly-HRP, Secondary Ab Dilution Ratio: 1:1500

Details

Detection NotesDetection Method: Chemiluminescence, Exposure Time: 30 seconds, Specific Bands: ~70 kD? kDa

Comments

CommentsWorks very well with chemiluminescent detection, but not quite strong enough for a good signal with colorimetric detection. Overall good! Appears slightly smaller than normal, although this can happen in my particular species. Only band observed on blot.

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for TLR4 Antibody (NB100-56566). (Showing 1 - 4 of 4 FAQs).

  1. In my experiment, I need to check the TLR4 expression on Swine embryonic fibroblast cells. Is it applicable to use the human TLR4 antibody? Have you obtained any references on swine cells which using the same TLR4 antibody.
    • This antibody has not been used on swine however I have carried out a blast and the % homology is 73% between the human protein and the pig protein. If you would be interested in testing this novel species, please take a look at our Innovators Reward Program.
  2. Do you know the amino acid sequence against which this antibody is directed to? And also, what isoform of the protein would it be recognizing, or from what specific TLR4 transcript?
    • This antibody binds within amino acids 100-200 of TLR4. This antibody will recognize isoforms 1 and 2 of TLR4 as this region is present in both of these isoforms, but not isoform 3, according to UniProt O00206.
  3. Have you tested whether NB100-56566/ NB100-56728 can detect TLR4/TLR2 from NBP2-26268/NBP2-26266, respectively? If you tested, could you please inform us about the western blot procedure?
    • These cell lines are designed for flow cytometry and TLR functional assays. They are not designed for western blot analysis of TLRs. The antibodies that you mentioned do recognize the TLRs in these cells. As shown on the NBP2-26266 data sheet, the TLR2 antibody can be used in flow cytometry to recognize the TLR2 in the NBP2-26266 cell line. As shown on the NBP2-26268 data sheet, the antibody can be used in flow cytometry to recognize the TLR4 in the NBP2-26268 cell line. Please note, in general it is not uncommon that antibodies do not recognize certain recombinant constructs in one or more techniques. Our cell lines were selected for functional activity rather than for recognition by particular TLR antibodies. The construct insert was confirmed by using antibody to HA tag as shown on the data sheets.
  4. Would you be able to share the sequence homology/alignment of the immunogen sequence of NB100-56566 to the Zebrafish protein (S5Q1H4 ( uniprot site))?
    • I ran the sequence you provided against the immunogen, and unfortunately, there is only a 28% homology.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NBP2-27149APC
Biotin Labeled NBP2-27149B
DyLight 350 Labeled NBP2-27149UV
DyLight 405 Labeled NBP2-27149V
DyLight 488 Labeled NBP2-27149G
DyLight 550 Labeled NBP2-27149R
DyLight 650 Labeled NBP2-27149C
DyLight 680 Labeled NBP2-27149FR
DyLight 755 Labeled NBP2-27149IR
HRP Labeled NBP2-27149H
PE Labeled NBP2-27149PE
PerCP Labeled NBP2-27149PCP

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Blogs on TLR4.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

TLR4 - A Guardian of Innate Immunity
Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

TLR4: Playing Roles in Apoptosis and Autoimmunity
TLR4 (Toll-like receptor 4) is a member of the Toll-like receptor (TLR) family that plays a key role in pathogen recognition and activation of innate immunity. Scientists have found that TLRs are highly conserved from Drosophila to humans, with a high...  Read full blog post.

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Recent Reviews

4.2
5
1
4
4
3
0
2
0
1
0

Anonymous
05/25/2017
Application:
Species: Mouse

Hayk Simonyan
05/16/2017
Application: IHC
Species: Mouse

Aileen Keating
05/12/2016
Application: WB
Species: Other

Bioinformatics

Gene Symbol TLR4
Entrez
Uniprot