TLR7 Antibody - BSA Free

Images

 
Immunohistochemistry: TLR7 Antibody [NBP2-24906] - The expression of TLR-7 in the hippocampal brain region. The immunofluorescence of TLR7 recognized by Alexa 488, green. GFAP recognized by Alexa 594, red. NEUN ...read more
Flow Cytometry: TLR7 Antibody [NBP2-24906] - An intracellular stain was performed on THP-1 cells with NBP2-24906 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then ...read more
Immunocytochemistry/ Immunofluorescence: TLR7 Antibody [NBP2-24906] - Immunolocalization of TLR7 (A) and glial fibrillary acidic protein (GFAP) (B) in murine myenteric plexus. Most of the GFAP positive cells were also ...read more
Immunohistochemistry-Frozen: TLR7 Antibody [NBP2-24906] - analysis of frozen mouse ear skin tissue using anti-TLR7 antibody. Image from verified customer review.
Immunohistochemistry: TLR7 Antibody [NBP2-24906] - Expression of the TLRs 5 and 7 were evaluated in the PDAC tissue by their staining intensity using TLR5 antibody (NBP2-24787) and TLR7 antibody (NBP2-24906). Note ...read more
Immunohistochemistry-Frozen: TLR7 Antibody [NBP2-24906] - analysis of TLR7 in acetone-fixed, frozen mouse ear skin section using anti-TLR7 antibody. Image from verified customer review.
Western Blot: TLR7 Antibody [NBP2-24906] - Analysis using the Azide Free version of NBP2-24906. Detection of TLR7 in RAW cell lysate using this antibody.
Immunocytochemistry/ Immunofluorescence: TLR7 Antibody [NBP2-24906] - Analysis using the Azide Free version of NBP2-24906. Staining of Raw 246.7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were ...read more
Immunohistochemistry-Paraffin: TLR7 Antibody - BSA Free [NBP2-24906] - Analysis of a FFPE tissue section of human skin using 1:200 dilution of TLR7 antibody (NBP2-24906). The staining was developed using HRP labeled ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, DB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, Bioactivity
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

TLR7 Antibody - BSA Free Summary

Immunogen
Partial synthetic peptide made to an internal portion of the human TLR7 protein (between amino acids 700-750) [UniProt 9NYK1]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TLR7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Dot Blot
  • Flow (Intracellular)
  • Flow Cytometry 2-5 ug/ 1x10^6 cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen 1:10-1:2000
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
  • Proximity Ligation Assay
  • Western Blot 1-3 ug/ml
Application Notes
Use in IHC-P (PMID: 25264223), IHC (PMID: 28219705), IP (PMID: 17452530), and Dot Blot (PMID:27248820) shown in scientific literature. Use in Proximity Ligation Assay reported in scientific literature (PMID: 30579042). Use in Dot blot reported in scientific literature (PMID: 27248820). Use in immunoprecipitation reported in scientific literature (PMID: 17452530). Use in Bioactivity reported in scientific literature (PMID:28894449). IHC-F reported in verified customer reviews.
Agonist
Reviewed Applications
Read 4 Reviews rated 5
using
NBP2-24906 in the following applications:

Publications
Read Publications using
NBP2-24906 in the following applications:

  • BA
    1 publication
  • DB
    1 publication
  • 7 publications
  • 2 publications
  • 1 publication
  • 17 publications
  • IHC
    9 publications
  • 2 publications
  • 10 publications
  • IP
    1 publication
  • PLA
    2 publications
  • SW
    1 publication
  • WB
    10 publications

Reactivity Notes

Use in Human reported in scientific literature (PMID:33806288).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TLR7 Antibody - BSA Free

  • TLR7
  • toll-like receptor 7

Background

Toll-like receptor 7 (TLR7) is a type I transmembrane protein expressed on the surface of endosomes and has a role in pathogen-associated molecular patterns (PAMPs) recognition and host defense (1-3). TLR7 is primarily expressed in the brain, placenta, spleen, stomach, and lungs (4). TLR7 recognizes microbial single stranded RNA (ssRNA), specifically guanosine and its derivatives (1-3). Human TLR7 cDNA encodes a 1049 amino acid (aa) protein with a theoretical molecular weight (MW) of 120.9 kDa (4). The TLR7 protein consists of a signal sequence, an 813 aa extracellular domain containing leucine-rich repeats (LRRs) which form a horseshoe-like shape, a 21 aa transmembrane domain, and a 189 aa cytoplasmic domain with cytosolic Toll-interleukin-1 receptor homology (TIR) domains (1,2,4). TLR7 and its fellow subfamily members, TLR8 and TLR9, possess a characteristic Z-loop between two LRRs with proteolytic Z-loop processing required for TLR activation (2). Z-loop cleavage in TLR7 allows for guanosine and uridine-rich ssRNA binding to the 1st and 2nd ligand binding site, respectively (2). The TIR domain associates with the adaptor protein myeloid differentiation primary response protein (MyD88) to initiate downstream signaling (1-3,5,6). Following activation by PAMPs, TLR7 dimerizes and bound MyD88 interacts with interleukin-1 receptor-associated kinase-4 (IRAK-4) (1,5). Together the complex recruits IRAK-1 and IRAK-2, which become phosphorylated, and interact with tumor necrosis factor receptor-associated factor 6 (TRAF6) (1,5). TRAF6 induces the activation of mitogen-activated protein kinase (MAPK), nuclear factor-kappaB (NF-kappaB), and interferon-regulatory factor 7 (IRF7), leading to interferon production and pro-inflammatory cytokine secretion associated with immune response (1,5).

While TLRs play an important role in innate immune response, dysfunction in the TLR-MyD88 signaling cascade has also been reported in various autoimmune disorders (5,6). Elevated expression of TLR7 is associated with increased risk of system lupus erythematosus (SLE), an autoimmune disease involving B cell hyperactivity (6,7). Studies involving mouse models has also found that increased TLR7 expression predisposes mice to a lupus-like disease (7). Therapeutics targeting TLR7 have been developed to either enhance or inhibit its activity depending on the circumstance. For example, TLR7 agonists such as imiquimod, resiquimod, and 852A are used to increase TLR7 activity for treatment of cancers and to fight viral infections (7,8). On the other hand, TLR7 antagonists inhibit its activation and have been developed to combat chronic immune stimulation as seen in inflammatory and autoimmune diseases (8).

References

1. Petes C, Odoardi N, Gee K. The Toll for Trafficking: Toll-Like Receptor 7 Delivery to the Endosome. Front Immunol. 2017;8:1075. https://doi.org/10.3389/fimmu.2017.01075

2. Maeda K, Akira S. TLR7 Structure: Cut in Z-Loop. Immunity. 2016;45(4):705-707. https://doi.org/10.1016/j.immuni.2016.10.003

3. Krieg AM, Vollmer J. Toll-like receptors 7, 8, and 9: linking innate immunity to autoimmunity. Immunol Rev. 2007;220:251-269. https://doi.org/10.1111/j.1600-065X.2007.00572.x

4. Uniprot (Q9NYK1)

5. Zheng C, Chen J, Chu F, Zhu J, Jin T. Inflammatory Role of TLR-MyD88 Signaling in Multiple Sclerosis. Front Mol Neurosci. 2020;12:314. https://doi.org/10.3389/fnmol.2019.00314

6. Chi H, Li C, Zhao FS, et al. Anti-tumor Activity of Toll-Like Receptor 7 Agonists. Front Pharmacol. 2017;8:304. https://doi.org/10.3389/fphar.2017.00304

7. Fillatreau S, Manfroi B, Dorner T. Toll-like receptor signalling in B cells during systemic lupus erythematosus. Nat Rev Rheumatol. 2021;17(2):98-108. https://doi.org/10.1038/s41584-020-00544-4

8. Patinote C, Karroum NB, Moarbess G, et al. Agonist and antagonist ligands of toll-like receptors 7 and 8: Ingenious tools for therapeutic purposes. Eur J Med Chem. 2020;193:112238. https://doi.org/10.1016/j.ejmech.2020.112238

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for TLR7 Antibody (NBP2-24906)(75)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, WB.

We have publications tested in 13 applications: BA, DB, Flow, Flow-IC, Human, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, SW, WB.


Filter By Application
BA
(1)
DB
(1)
Flow
(7)
Flow-IC
(2)
Human
(1)
ICC/IF
(17)
IHC
(9)
IHC-Fr
(2)
IHC-P
(10)
IP
(1)
PLA
(2)
SW
(1)
WB
(10)
All Applications
Filter By Species
Human
(32)
Mouse
(21)
Rat
(5)
WB
(1)
All Species
Showing Publications 1 - 10 of 75. Show All 75 Publications.
Publications using NBP2-24906 Applications Species
Hayakawa K, Fujishiro M, Yoshida Y et al. Exposure of female NZBWF1 mice to imiquimod induced lupus nephritis at an early age via a unique mechanism that differed from spontaneous onset Clinical and Experimental Immunology Feb 2 2022 [PMID: 35260898] (WB) WB
Zhu Y, Wu Z, Yan W et al. Allosteric inhibition of SHP2 uncovers aberrant TLR7 trafficking in aggravating psoriasis EMBO molecular medicine Dec 22 2021 [PMID: 34936223] (IHC-P, ICC/IF, Mouse, Human) IHC-P, ICC/IF Mouse, Human
Bortolotti D, Gentili V, Rizzo S et al. TLR3 and TLR7 RNA Sensor Activation during SARS-CoV-2 Infection Microorganisms Aug 26 2021 [PMID: 34576716] (Human, WB)

Details:
Citation using the Azide Free format of this antibody.
Human WB
Branchi V, Esser L, Boden C et al. A Combined TLR7/TLR9/GATA3 Score Can Predict Prognosis in Biliary Tract Cancer Diagnostics (Basel, Switzerland) Sep 1 2021 [PMID: 34573939] (IHC, Human) IHC Human
Proskocil BJ, Wai K, Lebold KM et al. TLR7 is expressed by support cells, but not sensory neurons, in ganglia Journal of neuroinflammation Sep 16 2021 [PMID: 34530852] (IHC-P, Mouse) IHC-P Mouse
Zhang L, Luo H, Meng W et al. Integration of flow cytometry and computational analysis to dissect the epidermal cellular subsets in keloids that correlate with recurrence The Journal of investigative dermatology Apr 8 2021 [PMID: 33839145] (IHC) IHC
Qin L, Zou J, Barnett A et al. TRAIL Mediates Neuronal Death in AUD: A Link between Neuroinflammation and Neurodegeneration International Journal of Molecular Sciences Mar 4 2021 [PMID: 33806288] (WB, IHC, Human) WB, IHC Human
Huang YL, Huang MT, Sung PS et al. Endosomal TLR3 co-receptor CLEC18A enhances host immune response to viral infection Communications biology Feb 18 2021 [PMID: 33603190] (ICC/IF, Human, Mouse) ICC/IF Human, Mouse
Zhang L, Cen Y, Huang Q et al. Computational Flow Cytometric Analysis to Detect Epidermal Subpopulations In Human Skin Biomed Eng Online Feb 18 2021 [PMID: 33596908]
Agier J, Brzezinska-Blaszczyk E, Witczak P et al. The impact of TLR7 agonist R848 treatment on mast cell phenotype and activity Cellular Immunology Oct 1 2020 [PMID: 33158544] (WB, Flow, Rat) WB, Flow Rat
Show All 75 Publications.

Reviews for TLR7 Antibody (NBP2-24906) (4) 54

Average Rating: 5
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Mouse, Rat.

Reviews using NBP2-24906:
Filter by Applications
IHC-Fr
(2)
WB
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(3)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen TLR7 NBP2-24906
Enlarge
5
reviewed by:
Musaad Alshammari
IHC-Fr Mouse 08/02/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedFixe mouse brain tissue
SpeciesMouse
Lot0221872A-3
Western Blot TLR7 NBP2-24906
Enlarge
5
reviewed by:
Hyun Yi
WB Rat 12/02/2017
View

Summary

ApplicationWestern Blot
Sample TestedDorsal root ganglion neurons
SpeciesRat
Lot043015
Immunohistochemistry-Frozen TLR7 NBP2-24906
Enlarge
5
reviewed by:
Anonymous A
IHC-Fr Mouse 08/29/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse Ear Skin (frozen section)
SpeciesMouse
Immunofluorescence TLR7 NBP2-24906
Enlarge
5
reviewed by:
Anonymous A
IF Mouse 08/28/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse Ear Skin (frozen section)
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TLR7 Antibody (NBP2-24906). (Showing 1 - 1 of 1 FAQs).

  1. Regarding antibody NBP2-24906 is the western blot of RAW264.7 cells detected with colorimetric staining? or is it luminescence (ECL)?
    • Image 5 of 13 is ECL

Secondary Antibodies

 

Isotype Controls

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By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria ...  Read full blog post.

NOX2 oxidase is a potential target for broad-spectrum antiviral therapies
By Beth Melson, MSViral infection induces the production of reactive oxygen species (ROS) by inflammatory cells, the majority of which is produced by the NOX2 oxidase. While NOX2 oxidase contributes to the clearan...  Read full blog post.

TLR9: For Whom the Cell Tolls
The Toll-like receptor 9 (TLR9) protein, also known as CD289, belongs to the family of Toll-like receptor (TLR) proteins which play a large role in pathogen recognition and the activation of innate immunity. Scientists using TLR9 antibodies have found...  Read full blog post.

TLR7 and Immune Response Regulation
Toll-like receptor 7 (TLR7) is a protein encoded by the TLR7 gene in humans and is a member of TLR family. TLRs controls host immune response against pathogens (e.g. viruses, bacteria and fungi) through recognition of pathogen-associated molecular pat...  Read full blog post.

TLR9 Antibodies in Immunity Research
Toll-like receptor 9 (TLR9) is a member of the toll-like receptor family that plays a key role in pathogen recognition and activation of innate immunity. Scientists using TLR9 antibodies have found the protein is highly conserved from Drosophila to hu...  Read full blog post.

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Recent Reviews

5
5
4
4
0
3
0
2
0
1
0

Musaad Alshammari
08/02/2019
Application: IHC-Fr
Species: Mouse

Hyun Yi
12/02/2017
Application: WB
Species: Rat

Anonymous A
08/29/2015
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol TLR7