TLR7 Antibody

Images

 
Flow Cytometry: TLR7 Antibody [NBP2-24906] - Analysis using the Azide Free version of NBP2-24906. Staining of TLR7 in human PBMC using this antibody at 0.5 ug/ml. Shaded histogram is cells alone, green is rabbit IgG ...read more
Flow (Intracellular): TLR7 Antibody [NBP2-24906] - Analysis using the FITC conjugate of NBP2-24906. Staining of TLR7 in human monocytes using this antibody at 0.5 ug/10^6 cells. Shaded histogram represents cells alone, ...read more
Immunocytochemistry/ Immunofluorescence: TLR7 Antibody [NBP2-24906] - Analysis using the Azide Free version of NBP2-24906. Staining of Raw 246.7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were ...read more
Immunofluorescence: TLR7 Antibody [NBP2-24906] - analysis of TLR7 in acetone-fixed, frozen mouse ear skin section using anti-TLR7 antibody. Image from verified customer review.
Immunohistochemistry-Frozen: TLR7 Antibody [NBP2-24906] - analysis of frozen mouse ear skin tissue using anti-TLR7 antibody. Image from verified customer review.
Western Blot: TLR7 Antibody [NBP2-24906] - Analysis using the Azide Free version of NBP2-24906. Detection of TLR7 in RAW cell lysate using this antibody.
Immunohistochemistry-Paraffin: TLR7 Antibody [NBP2-24906] - Analysis using the Azide Free version of NBP2-24906. Staining of human colon tissue using NBP2-24905 (top) and an isotype control (bottom) at 5 ug/ml.
Flow Cytometry: TLR7 Antibody [NBP2-24906] - Analysis using the FITC conjugate of NBP2-24906. Staining of TLR7 in Balb/c mouse splenocytes using this antibody. Green represents rabbit IgG isotype control ; red ...read more
Immunocytochemistry/ Immunofluorescence: TLR7 Antibody [NBP2-24906] - Immunolocalization of TLR7 (A) and glial fibrillary acidic protein (GFAP) (B) in murine myenteric plexus. Most of the GFAP positive cells were also ...read more
Immunocytochemistry/ Immunofluorescence: TLR7 Antibody [NBP2-24906] - Ramos cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Flow Cytometry: TLR7 Antibody [NBP2-24906] - Analysis using the FITC conjugate of NBP2-24906. Staining of TLR7 in TLR7/HEK 293 cells using NBP2-24777 at 1 ug/10^6 cells. Green represents Vector/HEK 293 cells; red ...read more
Flow Cytometry: TLR7 Antibody [NBP2-24906] - Analysis using the PE conjugate of NBP2-24906. Staining of PBMCs fixed and permeabilized using the intracellular staining kit and stained with 1 ug of TLR7-PE conjugate (red) ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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TLR7 Antibody Summary

Immunogen
This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 706-749 of human TLR7; GenBank no. gb|AAF78035.1|AF245702_1. It will cross-react with mouse TLR7. In human Ramos cells, additional bands are seen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TLR7
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Flow Cytometry 2-5 ug/ 1x10^6 cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:2000
  • Immunohistochemistry-Paraffin
  • Flow (Intracellular)
  • Immunofluorescence
Application Notes
Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 25264223). Use in Immunohistochemistry reported in scientific literature (PMID 28219705).
Agonist
Reviewed Applications
Read 3 Reviews rated 5
using
NBP2-24906 in the following applications:

Publications
Read Publications using
NBP2-24906 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TLR7 Antibody

  • TLR7
  • toll-like receptor 7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-24906
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Applications: WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, ICC/IF
Supplier Logo

Publications for TLR7 Antibody (NBP2-24906)(47)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 10 applications: DB, FLOW, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


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DB
(1)
FLOW
(1)
Flow
(5)
Flow-IC
(2)
ICC/IF
(11)
IHC
(1)
IHC-Fr
(2)
IHC-P
(6)
IP
(1)
WB
(4)
All Applications
Filter By Species
Human
(19)
Mouse
(13)
Rat
(2)
All Species
Showing Publications 1 - 10 of 47. Show All 47 Publications.
Publications using NBP2-24906 Applications Species
To EE, Vlahos R, Luong R et al. Endosomal NOX2 oxidase exacerbates virus pathogenicity and is a target for antiviral therapy Nat Commun 2017 Jul 12 [PMID: 28701733] (ICC/IF, Mouse) ICC/IF Mouse
Larsson O, Tengroth L, Xu Y et al. Substance P represents a novel first-line defense mechanism in the nose. J. Allergy Clin. Immunol. 2017 Feb 20 [PMID: 28219705] (Flow, ICC/IF, IHC, Mouse) Flow, ICC/IF, IHC Mouse
Daley D, Zambirinis CP, Seifert L et al. gammadelta T Cells Support Pancreatic Oncogenesis by Restraining alphabeta T Cell Activation. Cell Aug 25 2016 [PMID: 27569912] (Flow, Mouse, Human) Flow Mouse, Human
Li X, Liu F, Zhang X et al. Notch-Hes-1 axis controls TLR7-mediated autophagic death of macrophage via induction of P62 in mice with lupus Cell Death Dis Aug 19 2016 [PMID: 27537524] (Flow, Rat) Flow Rat
Huang AY, Wu SY. The effect of imiquimod on taste bud calcium transients and transmitter secretion. Br. J. Pharmacol. Jul 27 2016 [PMID: 27464850] (IHC-P, Human) IHC-P Human
Zeng M, Nouri-Shirazi E, Guinet E, Nouri-Shirazi M. The genetic background influences the cellular and humoral immune responses to vaccines Clin. Exp Immunol. [PMID: 27393001] (Flow, Mouse)

Details:
Citation using the Azide Free form of this antibody.
Flow Mouse
Jeon YK, Kim CK, Koh J et al. Pellino-1 confers chemoresistance in lung cancer cells by upregulating cIAP2 through Lys63-mediated polyubiquitination. Oncotarget. Jul 5 2016 [PMID: 27248820] (DB, Human) DB Human
Helley MP, Abate W, Jackson SK et al. The expression of Toll-like receptor 4, 7 and co-receptors in neurochemical sub-populations of rat trigeminal ganglion sensory neurons. Neuroscience. 2015 Dec 3 [PMID: 26434622] (ICC/IF, WB, Rat) ICC/IF, WB Rat
Renkonen J, Toppila-Salmi S, Joenvaara S et al. Expression of Toll-like receptors in nasal epithelium in allergic rhinitis APMIS. 2015 Jun 08 [PMID: 26061394] (IHC-P, Human)

Details:
TLR7 antibody (Imgenex IMG-581A) was used for IHC-P on nasal biopsies from healthy non-smoking volunteers both allergic to birch pollen and non-allergic controls. Nasal biopsies were collected before and after off-seasonal intranasal birch pollen or diluent challenge and the immunostaining assay implicated - 4um paraffin sections, antigen retrieval withTris-HCl pH 8.5, processing on Autostainer, use of primary at 1.67 ug/mL concentration, Rabbit/Mouse REAL EnVision Detection system and Peroxidase/DAB+ detection (Fig 1, Fig 2D ).
IHC-P Human
Renkonen S, Cardell LO, Mattila P et al. Toll-like receptors 3, 7, and 9 in Juvenile nasopharyngeal angiofibroma APMIS 2015 May 01 [PMID: 25912131] (IHC-P, Human)

Details:
TLR7 antibody was used for IHC-P application on formalin-fixed and paraffin-embedded sections from human juvenile nasopharyngeal angiofibroma/JNA patients and the immunoassay implicated : 4um sections, antigen retrieval via Lab Vision PT Module for 20 minutes at 98C, staining on Autostainer 480 with 1 hour primary incubation and REAL EnVision Detection System using REAL DAB+ Chromogen (Figure 2).
IHC-P Human
Show All 47 Publications.

Reviews for TLR7 Antibody (NBP2-24906) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Mouse, Rat.

Reviews using NBP2-24906:
Filter by Applications
WB
(1)
IHC-Fr
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(2)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot TLR7 NBP2-24906
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reviewed by:
Anonymous
WB Rat 12/02/2017
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Summary

ApplicationWestern Blot
Sample TestedDorsal root ganglion neurons
SpeciesRat
Lot043015
Immunohistochemistry-Frozen TLR7 NBP2-24906
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reviewed by:
Anonymous
IHC-Fr Mouse 08/29/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse Ear Skin (frozen section)
SpeciesMouse
Immunofluorescence TLR7 NBP2-24906
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reviewed by:
Anonymous
IF Mouse 08/28/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse Ear Skin (frozen section)
SpeciesMouse

Blocking

Blocking Details1xPBS+ 3%BSA+5% goat serum, room temperature 1hour

Primary Anitbody

Dilution Ratio1:400 dilution, 4C, overnight

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG, HRP
Secondary Concentration2.5ug/ml (1:200)

Details

Detection NotesZeiss microscope detection, rabbit IgG istotype control
Fixation Detailsacetone 5min fixation
Wash Description1xPBS imerse 3times, 5min each

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TLR7 Antibody (NBP2-24906) (0)

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Secondary Antibodies

 

Isotype Controls

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Diseases for TLR7 Antibody (NBP2-24906)

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Blogs on TLR7.

TLR9: For Whom the Cell Tolls
The Toll-like receptor 9 (TLR9) protein, also known as CD289, belongs to the family of Toll-like receptor (TLR) proteins which play a large role in pathogen recognition and the activation of innate immunity. Scientists using TLR9 antibodies have found...  Read full blog post.

TLR7 and Immune Response Regulation
Toll-like receptor 7 (TLR7) is a protein encoded by the TLR7 gene in humans and is a member of TLR family. TLRs controls host immune response against pathogens (e.g. viruses, bacteria and fungi) through recognition of pathogen-associated molecular pat...  Read full blog post.

TLR9 Antibodies in Immunity Research
Toll-like receptor 9 (TLR9) is a member of the toll-like receptor family that plays a key role in pathogen recognition and activation of innate immunity. Scientists using TLR9 antibodies have found the protein is highly conserved from Drosophila to hu...  Read full blog post.

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Anonymous
12/02/2017
Application: WB
Species: Rat

Anonymous
08/29/2015
Application: IHC-Fr
Species: Mouse

Anonymous
08/28/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol TLR7
Entrez