TLR7 Antibody

Images

 
Immunohistochemistry: TLR7 Antibody [NBP2-24906] - The expression of TLR-7 in the hippocampal brain region. The immunofluorescence of TLR7 recognized by Alexa 488, green. GFAP recognized by Alexa 594, red. NEUN ...read more
Flow Cytometry: TLR7 Antibody [NBP2-24906] - An intracellular stain was performed on THP-1 cells with TLR7 Antibody NBP2-24906 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and ...read more
Immunocytochemistry/ Immunofluorescence: TLR7 Antibody [NBP2-24906] - Immunolocalization of TLR7 (A) and glial fibrillary acidic protein (GFAP) (B) in murine myenteric plexus. Most of the GFAP positive cells were also ...read more
Immunohistochemistry-Frozen: TLR7 Antibody [NBP2-24906] - analysis of frozen mouse ear skin tissue using anti-TLR7 antibody. Image from verified customer review.
Immunohistochemistry: TLR7 Antibody [NBP2-24906] - Expression of the TLRs 5 and 7 were evaluated in the PDAC tissue by their staining intensity using TLR5 antibody (NBP2-24787) and TLR7 antibody (NBP2-24906). Note ...read more
Immunohistochemistry-Frozen: TLR7 Antibody [NBP2-24906] - analysis of TLR7 in acetone-fixed, frozen mouse ear skin section using anti-TLR7 antibody. Image from verified customer review.
Western Blot: TLR7 Antibody [NBP2-24906] - Analysis using the Azide Free version of NBP2-24906. Detection of TLR7 in RAW cell lysate using this antibody.
Immunocytochemistry/ Immunofluorescence: TLR7 Antibody [NBP2-24906] - Analysis using the Azide Free version of NBP2-24906. Staining of Raw 246.7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, DB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, Bioactivity
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

TLR7 Antibody Summary

Immunogen
This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 706-749 of human TLR7; GenBank no. |AAF78035.1, AF245702_1. Cross-reactivity with mouse TLR7 shown. In human Ramos cells, additional bands are also seen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TLR7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Dot Blot
  • Flow Cytometry 2-5 ug/ 1x10^6 cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:2000
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Proximity Ligation Assay
  • Bioactivity
  • Flow (Intracellular)
Application Notes
Use in IHC-P (PMID: 25264223), IHC (PMID: 28219705), IP (PMID: 17452530), and Dot Blot (PMID:27248820) shown in scientific literature. Use in Proximity Ligation Assay reported in scientific literature (PMID: 30579042). Use in Dot blot reported in scientific literature (PMID: 27248820). Use in immunoprecipitation reported in scientific literature (PMID: 17452530). Use in Bioactivity reported in scientific literature (PMID:28894449). IHC-F reported in verified customer reviews.
Agonist
Reviewed Applications
Read 4 Reviews rated 5
using
NBP2-24906 in the following applications:

Publications
Read Publications using
NBP2-24906 in the following applications:

  • BA
    1 publication
  • DB
    1 publication
  • 8 publications
  • 2 publications
  • 17 publications
  • IHC
    8 publications
  • 2 publications
  • 9 publications
  • IP
    1 publication
  • PLA
    2 publications
  • WB
    9 publications

Reactivity Notes

Use in Human reported in scientific literature (PMID:33806288).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TLR7 Antibody

  • TLR7
  • toll-like receptor 7

Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described. Stimulation of the NFkB signaling pathway by TLR7 suggests that it plays a role in immune response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-24917
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Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, BA, B/N, Flow-IC
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Applications: WB, ChIP, DB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, PAGE, B/N, CyTOF-ready, Dual ISH-IHC, ELISA(Cap), Flow-CS, Flow-IC, KD, KO
NB100-56722
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, DB, ELISA, Flow, Func, ICC/IF, IP, In vitro, B/N, Flow-CS
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Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
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NBP2-24787
Species: Hu, Mu, Ca
Applications: WB, DB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-CS, Flow-IC
NBP1-72042
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NB100-56563
Species: Hu, Mu, Rt
Applications: WB, DB, Flow, IHC, IHC-P, Flow-CS, Flow-IC
NB100-56583
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Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
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Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Dual ISH-IHC
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Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
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Publications for TLR7 Antibody (NBP2-24906)(70)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 11 applications: BA, DB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, WB.


Filter By Application
BA
(1)
DB
(1)
Flow
(8)
Flow-IC
(2)
ICC/IF
(17)
IHC
(8)
IHC-Fr
(2)
IHC-P
(9)
IP
(1)
PLA
(2)
WB
(9)
All Applications
Filter By Species
Human
(31)
Mouse
(18)
Rat
(6)
All Species
Showing Publications 1 - 10 of 70. Show All 70 Publications.
Publications using NBP2-24906 Applications Species
Zhang L, Luo H, Meng W et al. Integration of flow cytometry and computational analysis to dissect the epidermal cellular subsets in keloids that correlate with recurrence The Journal of investigative dermatology Apr 8 2021 [PMID: 33839145] (IHC) IHC
Qin L, Zou J, Barnett A et al. TRAIL Mediates Neuronal Death in AUD: A Link between Neuroinflammation and Neurodegeneration International Journal of Molecular Sciences Mar 4 2021 [PMID: 33806288] (WB, IHC, Human) WB, IHC Human
Huang YL, Huang MT, Sung PS et al. Endosomal TLR3 co-receptor CLEC18A enhances host immune response to viral infection Communications biology Feb 18 2021 [PMID: 33603190] (ICC/IF, Human, Mouse) ICC/IF Human, Mouse
Agier J, Brzezinska-Blaszczyk E, Witczak P et al. The impact of TLR7 agonist R848 treatment on mast cell phenotype and activity Cellular Immunology Oct 1 2020 [PMID: 33158544] (WB, Flow, Rat) WB, Flow Rat
Karadimou G, Plunde O, Pawelzik SC et al. TLR7 Expression Is Associated with M2 Macrophage Subset in Calcific Aortic Valve Stenosis Cells Jul 16 2020 [PMID: 32708790] (ICC/IF, Human) ICC/IF Human
Hoshikawa N, Sakai A et al. Targeting Extracellular miR-21-TLR7 Signaling Provides Long-Lasting Analgesia in Osteoarthritis. Mol Ther Nucleic Acids Jun 3 2020 [PMID: 31841992] (ICC/IF, Rat) ICC/IF Rat
Alshammari TK, Alghamdi H, Green TA et al. Assessing the role of toll-like receptor in isolated, standard and enriched housing conditions PLoS ONE 2019 Oct 24 [PMID: 31647818] (ICC/IF, Mouse) ICC/IF Mouse
Gu R, Huang T, Xiao J et al. The IRE1 alpha Arm of UPR Regulates Muscle Cells Immune Characters by Restraining p38 MAPK Activation Front Physiol 2019 Sep 19 [PMID: 31607947] (WB, Mouse) WB Mouse
Huang Y, Zhou F, Xiao Y et al. TLR7 mediates increased vulnerability to ischemic acute kidney injury in diabetes Rev Assoc Med Bras (1992) Sep 12 2019 [PMID: 31531603] (WB, Human) WB Human
Kasurinen A, Hagstrom J, Laitinen A et al. Evaluation of toll-like receptors as prognostic biomarkers in gastric cancer: high tissue TLR5 predicts a better outcome Sci Rep [PMID: 31467388] (IHC-P, Human) IHC-P Human
Show All 70 Publications.

Reviews for TLR7 Antibody (NBP2-24906) (4) 54

Average Rating: 5
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Mouse, Rat.

Reviews using NBP2-24906:
Filter by Applications
IHC-Fr
(2)
WB
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(3)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen TLR7 NBP2-24906
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Mouse 08/02/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedFixe mouse brain tissue
SpeciesMouse
Lot0221872A-3
Western Blot TLR7 NBP2-24906
Enlarge
5
reviewed by:
Verified Customer
WB Rat 12/02/2017
View

Summary

ApplicationWestern Blot
Sample TestedDorsal root ganglion neurons
SpeciesRat
Lot043015
Immunohistochemistry-Frozen TLR7 NBP2-24906
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Mouse 08/29/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse Ear Skin (frozen section)
SpeciesMouse
Immunofluorescence TLR7 NBP2-24906
Enlarge
5
reviewed by:
Verified Customer
IF Mouse 08/28/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse Ear Skin (frozen section)
SpeciesMouse

Blocking

Blocking Details1xPBS+ 3%BSA+5% goat serum, room temperature 1hour

Primary Anitbody

Dilution Ratio1:400 dilution, 4C, overnight

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG, HRP
Secondary Concentration2.5ug/ml (1:200)

Details

Detection NotesZeiss microscope detection, rabbit IgG istotype control
Fixation Detailsacetone 5min fixation
Wash Description1xPBS imerse 3times, 5min each

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TLR7 Antibody (NBP2-24906). (Showing 1 - 1 of 1 FAQs).

  1. Regarding antibody NBP2-24906 is the western blot of RAW264.7 cells detected with colorimetric staining? or is it luminescence (ECL)?
    • Image 5 of 13 is ECL

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-24906AF350
Alexa Fluor 405 NBP2-24906AF405
Alexa Fluor 488 NBP2-24906AF488
Alexa Fluor 532 NBP2-24906AF532
Alexa Fluor 594 NBP2-24906AF594
Alexa Fluor 647 NBP2-24906AF647
Alexa Fluor 700 NBP2-24906AF700
Alexa Fluor 750 NBP2-24906AF750
Allophycocyanin NBP2-24906APC
Biotin NBP2-24906B
DyLight 350 NBP2-24906UV
DyLight 405 NBP2-24906V
DyLight 488 NBP2-24906G
DyLight 550 NBP2-24906R
DyLight 594 NBP2-24906DL594
DyLight 650 NBP2-24906C
DyLight 680 NBP2-24906FR
DyLight 755 NBP2-24906IR
FITC NBP2-24777
HRP NBP2-24906H
Janelia Fluor 549 NBP2-24906JF549
Janelia Fluor 646 NBP2-24906JF646
PE NBP2-24906PE
PE NBP2-31071
PerCP NBP2-24906PCP

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Bioinformatics Tool for TLR7 Antibody (NBP2-24906)

Discover related pathways, diseases and genes to TLR7 Antibody (NBP2-24906). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Pathways for TLR7 Antibody (NBP2-24906)

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Research Areas for TLR7 Antibody (NBP2-24906)

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Blogs on TLR7.


  Read full blog post.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

NOX2 oxidase is a potential target for broad-spectrum antiviral therapies
By Beth Melson, MSViral infection induces the production of reactive oxygen species (ROS) by inflammatory cells, the majority of which is produced by the NOX2 oxidase. While NOX2 oxidase contributes to the clearan...  Read full blog post.

TLR9: For Whom the Cell Tolls
The Toll-like receptor 9 (TLR9) protein, also known as CD289, belongs to the family of Toll-like receptor (TLR) proteins which play a large role in pathogen recognition and the activation of innate immunity. Scientists using TLR9 antibodies have found...  Read full blog post.

TLR7 and Immune Response Regulation
Toll-like receptor 7 (TLR7) is a protein encoded by the TLR7 gene in humans and is a member of TLR family. TLRs controls host immune response against pathogens (e.g. viruses, bacteria and fungi) through recognition of pathogen-associated molecular pat...  Read full blog post.

TLR9 Antibodies in Immunity Research
Toll-like receptor 9 (TLR9) is a member of the toll-like receptor family that plays a key role in pathogen recognition and activation of innate immunity. Scientists using TLR9 antibodies have found the protein is highly conserved from Drosophila to hu...  Read full blog post.

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Recent Reviews

5
5
4
4
0
3
0
2
0
1
0

Verified Customer
08/02/2019
Application: IHC-Fr
Species: Mouse

Verified Customer
12/02/2017
Application: WB
Species: Rat

Verified Customer
08/29/2015
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol TLR7