p62/SQSTM1 Antibody


Western Blot: p62/SQSTM1 Antibody [NBP1-48320] - Cultured HeLa cells were treated with or without 50 uM chloriquine for 24 hours as indicated. Cell lysates were prepared and separated on a 12% gel by SDS-PAGE. Protein ...read more
Immunocytochemistry/ Immunofluorescence: p62/SQSTM1 Antibody [NBP1-48320] - Immunocytochemical analysis of SQSTM1/p62 in HeLa cells
Simple Western: p62/SQSTM1 Antibody [NBP1-48320] - Simple Western lane view shows a specific band for p62/SQSTM1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
0.2 mg/ml

Order Details

p62/SQSTM1 Antibody Summary

A genomic peptide made to c-t of the human p62/SQSTM1 protein (within residues 300-440). [Swiss-Prot Q13501]
Cytoplasm. Late endosome. Nucleus.
Predicted Species
Porcine (96%), Canine (91%), Chinese Hamster (98%), Bovine (97%), Sheep (98%), Invertebrate (97%)
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
PBS, 0.1% BSA, 30% glycerol
0.05% Sodium Azide
0.2 mg/ml
Immunogen affinity purified


Application Notes
Use of this antibody in IHC-Frozen is reported in PMID 25014022 and in IHC-paraffin is reported in PMID 27497324. In Simple Western, only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 3 Reviews rated 4.3
NBP1-48320 in the following application:

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NBP1-48320 in the following applications:

Reactivity Notes

Human, Mouse and Rat. Immunogen sequence is 100% identical to several non-human primates/monkey species. Immunogen sequence homology to other species: Chinese hamster (98%), Sheep (98%), Bovine (97%), Porcine (96%), Canine (91%), Daphnia magna, a cladoceran/crustaceans invertebrate (97%) and Duck and several other Birds (84%).


Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for p62/SQSTM1 Antibody

  • A170
  • EBI3-associated protein of 60 kDa
  • EBI3-associated protein p60
  • ORCA
  • OSIL
  • oxidative stress induced like
  • p60PDB3
  • p62
  • p62B
  • Paget disease of bone 3
  • PDB3
  • phosphotyrosine independent ligand for the Lck SH2 domain p62
  • Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
  • Sequestosome 1
  • sequestosome-1
  • SQSTM1
  • Ubiquitin-binding protein p62
  • ZIP3


p62/SQSTM1 (sequestosome 1) is ubiquitously-expressed cytoplasmic/adapter protein conserved in vertebrates and it can be induced by proteasomal inhibitor PSI, PGJ2/prostaglandin J2 as well as phorbol 12-myristate 13-acetate (PMA). SQSTM1 has the ability of ubiquitin binding as well as regulating NFkB1 activation by TNF-alpha, NGF (nerve growth factor) and interleukin-1. SQSTM1 acts as an adapter that mediates the interaction between TRAF6 and CYLD, and SQSTM1-TRAF6 interaction leads to K63-linked polyubiquitination of TRAF6 followed by subsequent activation of NFkB pathway. SQSTM1 plays a role in TITIN/TTN downstream signaling in muscle cells and regulate signaling cascades through ubiquitination. SQSTM1 is essential both for formation and autophagic degradation of polyubiquitin-containing bodies which are known as aggresome-like induced structures (ALIS) and SQSTM1 connects ALIS to the autophagic machinery via direct interaction with MAP1 LC3 family members. Moreover, while autophagy modulates SQSTM1 protein levels, SQSTM1 suppresses autophagy via activation of mTORC1. SQSTM1 interacts with KEAP1, which is a cytoplasmic inhibitor of NRF2, a key transcription factor involved in cellular responses to oxidative stress. SQSTM1 functions as a signaling hub for various signal transduction pathways, apoptosis, cell differentiation, apoptosis, immune response, regulation of K+ channels and Nrf2 activation, and its dysregulation is associated with Paget disease of bone and tumorigenesis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu
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Species: Hu, Mu, Rt, Bovine, Canine, Porcine, Sheep, Invertebrate, Chinese Hamster
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Publications for p62/SQSTM1 Antibody (NBP1-48320)(23)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC-Fr, IHC-P, WB.

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Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using NBP1-48320 Applications Species
Tan KP, Ho MY, Cho HC et al. Fucosylation of LAMP-1 and LAMP-2 by FUT1 correlates with lysosomal positioning and autophagic flux of breast cancer cells. Cell Death Dis. 2016 Aug 25 [PMID:27560716] (WB, Human) WB Human
Katzenell S, Leib DA. Herpes simplex virus and interferon signaling induce novel autophagic clusters in sensory neurons J. Virol. Feb 24 2016 12:00AM [PMID:26912623] (ICC/IF, Mouse) ICC/IF Mouse
Schwab AJ, Ebert AD. Neurite Aggregation and Calcium Dysfunction in iPSC-Derived Sensory Neurons with Parkinson's Disease-Related LRRK2 G2019S Mutation. Stem Cell Reports. 2015 Dec 08 [PMID:26651604] (WB, Human) WB Human
Dever SM, Rodriguez M, Lapierre J et al. Differing roles of autophagy in HIV-associated neurocognitive impairment and encephalitis with implications for morphine co-exposure. Front Microbiol 2015 [PMID:26217309] (ICC/IF) ICC/IF
Chia-Wei Lin, Ming-Shiou Jan, Jung-Hua Steven Kuo. Exploring MicroRNA Expression Profiles Related to the mTOR Signaling Pathway in Mouse Embryonic Fibroblast Cells Treated with Polyethylenimine. Mol Pharm. 2015 Aug 3 [PMID:26158199]
Yao J, Jia L, Khan N et al. Deletion of autophagy inducer RB1CC1 results in degeneration of the retinal pigment epithelium Autophagy. 2015 Jun 03 [PMID:26075877] (WB, Mouse) WB Mouse
Lin HH. In Vitro and in Vivo Atheroprotective Effects of Gossypetin against Endothelial Cell Injury by Induction of Autophagy. Chem Res Toxicol. 2015 Jan 29 [PMID:25622137]
Sun KT, Chen MY, Tu MG et al. MicroRNA-20a regulates autophagy related protein-ATG16L1 in hypoxia-induced osteoclast differentiation. Bone. 2014 Dec 05 [PMID:25485521] (WB, Mouse) WB Mouse
Rozman S, Yousefi S, Oberson K et al. The generation of neutrophils in the bone marrow is controlled by autophagy. Cell Death Differ. 2014 Oct 17 [PMID:25323583] (WB, Mouse) WB Mouse
Lin CW, Jan MS, Kuo JH. Autophagy-related gene expression analysis of wild-type and atg5 gene knockout mouse embryonic fibroblast cells treated with polyethylenimine. Mol Pharm. 2014 Sep 2 [PMID:25075440]
Show All 23 Publications.

Reviews for p62/SQSTM1 Antibody (NBP1-48320) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.
We have 3 review tested in 1 application: WB.

Reviews using NBP1-48320:
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Images Ratings Applications Species Date Details
reviewed by:
WB Human 12/23/2014


ApplicationWestern Blot
Sample TestedSee PMID 23931340


Blocking DetailsSee PMID 23931340


Detection NotesSee PMID 23931340


CommentsIndependent review from antibodyresource.comPublished in PMID 23931340.
Western Blot p62/SQSTM1 NBP1-48320
reviewed by:
WB Mouse 11/15/2013


ApplicationWestern Blot
Pub Med ID23939536
FileView PDF
reviewed by:
WB Human 12/23/2011


ApplicationWestern Blot
Sample TestedHuman


Blocking DetailsBlocking Buffer: 5% Milk in TBST

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:10000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab Dilution Ratio: 1:10000


Detection NotesDetection Method: ECL

Product General Protocols

View specific protocols for p62/SQSTM1 Antibody (NBP1-48320): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for p62/SQSTM1 Antibody (NBP1-48320). (Showing 1 - 1 of 1 FAQs).

  1. This technical query is related to Cat. No.: NBP1-48320. Technical Concern: As per the technical data sheet, p62/ SQSTM1 band is seen at ~47 kDa. We expect the band to be seen at 62 KDa for the domain p62/ SQSTM1, however only a ~47kDa band is visible in our blot, is there any explanation for this observation?
    • Regarding your concern with the performance of our p62 antibody (NBP1-48320), it is expected to see multiple bands depending on what form of p62 is present in the testing sample. According to uniprot, p62 has two different isoforms at 47kDa and 38kDa, however due to the interaction of p62 with other proteins and some possible modifications, a 62kDa band is usually observed on the blot. There is an abundance of supporting literature suggesting this antibody is performing well. p62 is considered to be the marker for Autophagy and the protein undergoes massive post translational modifications which can explain size increase. There are 13 phosphorylation sites on this protein. Here is the uniprot link for p62 (Q13501 reference 30); (PubMed 15592455).

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Blogs on p62/SQSTM1.

ATG4C - A regulator of the early steps of autophagosome assembly
Autophagy is an important cellular process that maintains homeostasis by degrading and recycling damaged proteins and organelles. Autophagy receptors, such as p62/SQSTM1, recognize these intracellular cargo and mediate their engulfment by the doubl...  Read full blog post.

p62/SQSTM1 - targeting ubiquitinated proteins for autophagic degradation
During autophagy ubiquitinated cargo or substrates are engulfed in a double-membrane autophagosome and transported to the lysosome for degradation. This process is important for maintaining cellular homeostasis and for degrading damaged organelles...  Read full blog post.

LC3/LC3B - measuring autophagosome formation and autophagic flux
Microtubule-associated protein-1 light chain 3 (LC3/LC3B) is a ubiquitin-like protein involved in the formation of the autophagosome. It is homologous to the yeast Atg8 protein. Autophagosomes are important for the degradation and recycling of intr...  Read full blog post.

p62/SQSTM1 (sequestosome 1)
p62/SQSTM1 (sequestosome 1) is ubiquitously-expressed cytoplasmic/adaptor protein. SQSTM1 functions as a signaling hub for various signal transduction pathways involved in apoptosis, cell differentiation, apoptosis, immune response, and K+ channel reg...  Read full blog post.

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Gene Symbol SQSTM1

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