p62/SQSTM1 Antibody

Images

 
Western Blot: p62/SQSTM1 Antibody [NBP1-48320] - Cultured HeLa cells were treated with or without 50 uM chloriquine for 24 hours as indicated. Cell lysates were prepared and separated on a 12% gel by SDS-PAGE. Protein ...read more
Immunocytochemistry/ Immunofluorescence: p62/SQSTM1 Antibody [NBP1-48320] - HeLa cells were treated overnight with 50uM CQ, then fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS ...read more
Immunocytochemistry/ Immunofluorescence: p62/SQSTM1 Antibody [NBP1-48320] - Untreated HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The ...read more
Immunocytochemistry/ Immunofluorescence: p62/SQSTM1 Antibody [NBP1-48320] - ICC/IF analysis of formalin fixed HeLa cells using SQSTM1/p62 antibody. Blocking was performed using 10% normal goat serum and the primary ...read more
Simple Western: p62/SQSTM1 Antibody [NBP1-48320] - Simple Western lane view shows a specific band for p62/SQSTM1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, ChHa, In, ShSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

p62/SQSTM1 Antibody Summary

Immunogen
A genomic peptide made to c-t of the human p62/SQSTM1 protein (within residues 300-440). [Swiss-Prot Q13501]
Localization
Cytoplasm. Late endosome. Nucleus.
Predicted Species
Porcine (96%), Bovine (97%), Chinese Hamster (98%), Canine (91%), Invertebrate (97%), Sheep (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SQSTM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:25
  • Immunocytochemistry/Immunofluorescence 1:25-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Use of this antibody in IHC-Frozen is reported in PMID 25014022 and in IHC-paraffin is reported in PMID 27497324.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
Biliverdin Reductase A/BLVRA Antibody (H00000644-M01)
Reviewed Applications
Read 6 Reviews rated 4.7
using
NBP1-48320 in the following applications:

Publications
Read Publications using
NBP1-48320 in the following applications:

Reactivity Notes

Human, Mouse and Rat. Immunogen sequence is 100% identical to several non-human primates/monkey species. Immunogen sequence homology to other species: Chinese hamster (98%), Sheep (98%), Bovine (97%), Porcine (96%), Canine (91%), Daphnia magna, a cladoceran/crustaceans invertebrate (97%) and Duck and several other Birds (84%).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for p62/SQSTM1 Antibody

  • A170
  • EBI3-associated protein of 60 kDa
  • EBI3-associated protein p60
  • EBIAP
  • ORCA
  • OSIL
  • oxidative stress induced like
  • p60PDB3
  • p62
  • p62B
  • Paget disease of bone 3
  • PDB3
  • phosphotyrosine independent ligand for the Lck SH2 domain p62
  • Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
  • Sequestosome 1
  • sequestosome-1
  • SQSTM1
  • Ubiquitin-binding protein p62
  • ZIP3

Background

p62/SQSTM1 (sequestosome 1) is ubiquitously-expressed cytoplasmic/adapter protein conserved in vertebrates and it can be induced by proteasomal inhibitor PSI, PGJ2/prostaglandin J2 as well as phorbol 12-myristate 13-acetate (PMA). SQSTM1 has the ability of ubiquitin binding as well as regulating NFkB1 activation by TNF-alpha, NGF (nerve growth factor) and interleukin-1. SQSTM1 acts as an adapter that mediates the interaction between TRAF6 and CYLD, and SQSTM1-TRAF6 interaction leads to K63-linked polyubiquitination of TRAF6 followed by subsequent activation of NFkB pathway. SQSTM1 plays a role in TITIN/TTN downstream signaling in muscle cells and regulate signaling cascades through ubiquitination. SQSTM1 is essential both for formation and autophagic degradation of polyubiquitin-containing bodies which are known as aggresome-like induced structures (ALIS) and SQSTM1 connects ALIS to the autophagic machinery via direct interaction with MAP1 LC3 family members. Moreover, while autophagy modulates SQSTM1 protein levels, SQSTM1 suppresses autophagy via activation of mTORC1. SQSTM1 interacts with KEAP1, which is a cytoplasmic inhibitor of NRF2, a key transcription factor involved in cellular responses to oxidative stress. SQSTM1 functions as a signaling hub for various signal transduction pathways, apoptosis, cell differentiation, apoptosis, immune response, regulation of K+ channels and Nrf2 activation, and its dysregulation is associated with Paget disease of bone and tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-48320
Species: Hu, Mu, Rt, Po, Bv, Ca, ChHa, In, Sh
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for p62/SQSTM1 Antibody (NBP1-48320)(34)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(5)
IHC-Fr
(1)
IHC-P
(1)
WB
(26)
All Applications
Filter By Species
Human
(13)
Mouse
(14)
Rat
(1)
All Species
Showing Publications 1 - 10 of 34. Show All 34 Publications.
Publications using NBP1-48320 Applications Species
Jiang P, Gan M, Yen SH et al. Impaired endo-lysosomal membrane integrity accelerates the seeding progression of a-synuclein aggregates Sci Rep 2017 Aug 09 [PMID: 28794446] (ICC/IF, Human) ICC/IF Human
Lee KH, Lee S, Lee HJ et al. Amyloid B1-42 (AB1-42) Induces the CDK2-Mediated Phosphorylation of Tau through the Activation of the mTORC1 Signaling Pathway While Promoting Neuronal Cell Death Front. Mol. Neurosci. 2017 Jul 24 [PMID: 28790888] (WB, Human) WB Human
Shen L, Yang Y, Ou T et al. Dietary PUFAs attenuate NLRP3 inflammasome activation via enhancing macrophage autophagy J. Lipid Res. 2017 Jul 20 [PMID: 28729463] (WB, Mouse) WB Mouse
Germic N, Stojkov D, Oberson K, et al. Neither eosinophils nor neutrophils require ATG5-dependent autophagy for extracellular DNA trap formation Immunology 2017 Jul 13 [PMID: 28703297] (Mouse) Mouse
Kim Y, Jeong EI, Nah J et al. Pimozide reduces toxic forms of tau in TauC3 mice via AMPK-mediated autophagy. J. Neurochem. 2017 Jun 20 [PMID: 28632947] (WB) WB
Su HH, Chu YC, Liao JM et al. Phellinus linteus Mycelium Alleviates Myocardial Ischemia-Reperfusion Injury through Autophagic Regulation. Front Pharmacol. Apr 19 2017 [PMID: 28420993] (WB, Rat) WB Rat
Eritja N, Chen BJ, Rodriguez-Barrueco R et al. Autophagy orchestrates adaptive responses to targeted therapy in endometrial cancer Autophagy Jan 5 2017 [PMID: 28055301] (WB, Human) WB Human
Kim DI, Lee KH, Gabr AA, et al. A beta-Induced Drp1 phosphorylation through Akt activation promotes excessive mitochondrial fission leading to neuronal apoptosis Biochim Biophys Acta. [PMID: 27599716] (WB, Human) WB Human
Tan KP, Ho MY, Cho HC et al. Fucosylation of LAMP-1 and LAMP-2 by FUT1 correlates with lysosomal positioning and autophagic flux of breast cancer cells. Cell Death Dis. Aug 26 2016 [PMID: 27560716] (WB, Human) WB Human
Sidjanin DJ, Park AK, Ronchetti A et al. TBC1D20 mediates autophagy as a key regulator of autophagosome maturation. Autophagy. Aug 3 2016 [PMID: 27487390] (WB, Mouse) WB Mouse
Show All 34 Publications.

Reviews for p62/SQSTM1 Antibody (NBP1-48320) (6) 4.76

Average Rating: 4.7
(Based on 6 reviews)
We have 6 reviews tested in 3 species: Human, Mouse, human.
We have 6 reviews tested in 3 application: WB, ICC, IHC-P.

Reviews using NBP1-48320:
Filter by Applications
WB
(4)
ICC
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(4)
Mouse
(1)
human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot p62/SQSTM1 NBP1-48320
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reviewed by:
Anonymous
WB Human 10/30/2017
View

Summary

ApplicationWestern Blot
Sample Testedhead and neck squamous cell carcinoma,Human HNSCC cell lines CAL27
SpeciesHuman
LotNBP1-48320
Immunocytochemistry p62/SQSTM1 NBP1-48320
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reviewed by:
Judit Herreros
ICC Human 09/01/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedU87MG cells treated with chloroquine
SpeciesHuman
LotNBP1-48320SS
Immunohistochemistry-Paraffin p62/SQSTM1 NBP1-48320
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reviewed by:
Judit Herreros
IHC-P human 09/01/2017
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman U87MG glioblastoma cells xenograft following cloroquine treatment
Specieshuman
LotNBP1-48320SS
 
reviewed by:
Anonymous
WB Human 12/23/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23931340
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23931340

Details

Detection NotesSee PMID 23931340

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 23931340.
Western Blot p62/SQSTM1 NBP1-48320
Enlarge
reviewed by:
Anonymous
WB Mouse 11/15/2013
View

Summary

ApplicationWestern Blot
SpeciesMouse
Pub Med ID23939536
FileView PDF
 
reviewed by:
Anonymous
WB Human 12/23/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in TBST

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:10000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab Dilution Ratio: 1:10000

Details

Detection NotesDetection Method: ECL

Product General Protocols

View specific protocols for p62/SQSTM1 Antibody (NBP1-48320): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for p62/SQSTM1 Antibody (NBP1-48320). (Showing 1 - 1 of 1 FAQs).

  1. This technical query is related to Cat. No.: NBP1-48320. Technical Concern: As per the technical data sheet, p62/ SQSTM1 band is seen at ~47 kDa. We expect the band to be seen at 62 KDa for the domain p62/ SQSTM1, however only a ~47kDa band is visible in our blot, is there any explanation for this observation?
    • Regarding your concern with the performance of our p62 antibody (NBP1-48320), it is expected to see multiple bands depending on what form of p62 is present in the testing sample. According to uniprot, p62 has two different isoforms at 47kDa and 38kDa, however due to the interaction of p62 with other proteins and some possible modifications, a 62kDa band is usually observed on the blot. There is an abundance of supporting literature suggesting this antibody is performing well. p62 is considered to be the marker for Autophagy and the protein undergoes massive post translational modifications which can explain size increase. There are 13 phosphorylation sites on this protein. Here is the uniprot link for p62 (Q13501 reference 30); (PubMed 15592455).

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-48320AF405
Alexa Fluor 488 Labeled NBP1-48320AF488
Alexa Fluor 647 Labeled NBP1-48320AF647
Alexa Fluor 700 Labeled NBP1-48320AF700
Biotin Labeled NBP1-48320B
DyLight 488 Labeled NBP1-48320G
DyLight 550 Labeled NBP1-48320R
DyLight 650 Labeled NBP1-48320C
HRP Labeled NBP1-48320H

Additional p62/SQSTM1 Products

Bioinformatics Tool for p62/SQSTM1 Antibody (NBP1-48320)

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PTMs for p62/SQSTM1 Antibody (NBP1-48320)

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Research Areas for p62/SQSTM1 Antibody (NBP1-48320)

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Blogs on p62/SQSTM1.

Key Targets in Apoptosis, Necroptosis, and Autophagy
Cell death/recycling pathways such as apoptosis, necroptosis, and autophagy are an integral part of the growth, development, homeostasis as well as the pathophysiology in the life of living organisms. These signaling pathways are highly regulated and...  Read full blog post.

Required proteins for p62/SQSTM1 regulation and a role for p62/SQSTM1 in neuronal autophagy
Autophagy is a crucial cellular process that clears the cell of protein aggregates, toxins, and damaged cell products. Accumulation of toxins, damaged cell products and unwanted proteins has been proven to play a role in aging and many forms of dis...  Read full blog post.

ATG4C - A regulator of the early steps of autophagosome assembly
Autophagy is an important cellular process that maintains homeostasis by degrading and recycling damaged proteins and organelles. Autophagy receptors, such as p62/SQSTM1, recognize these intracellular cargo and mediate their engulfment by the doubl...  Read full blog post.

p62/SQSTM1 - targeting ubiquitinated proteins for autophagic degradation
During autophagy ubiquitinated cargo or substrates are engulfed in a double-membrane autophagosome and transported to the lysosome for degradation. This process is important for maintaining cellular homeostasis and for degrading damaged organelles...  Read full blog post.

LC3/LC3B - measuring autophagosome formation and autophagic flux
Microtubule-associated protein-1 light chain 3 (LC3/LC3B) is a ubiquitin-like protein involved in the formation of the autophagosome. It is homologous to the yeast Atg8 protein. Autophagosomes are important for the degradation and recycling of intr...  Read full blog post.

p62/SQSTM1 (sequestosome 1)
p62/SQSTM1 (sequestosome 1) is ubiquitously-expressed cytoplasmic/adaptor protein. SQSTM1 functions as a signaling hub for various signal transduction pathways involved in apoptosis, cell differentiation, apoptosis, immune response, and K+ channel reg...  Read full blog post.

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Recent Reviews

4.7
5
4
4
2
3
0
2
0
1
0

Anonymous
10/30/2017
Application: WB
Species: Human

Judit Herreros
09/01/2017
Application: ICC
Species: Human

Judit Herreros
09/01/2017
Application: IHC-P
Species: human

Bioinformatics

Gene Symbol SQSTM1
Entrez
Uniprot