Src [p Tyr419] Antibody [Unconjugated]

Images

 
Western blot shows lysates of MDA‑MB‑468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 50 μM pervanadate (PV) for 10 minutes. PVDF membrane was ...read more
Src phosphorylated at Y419 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Src phosphorylated at Y419 was detected in immersion fixed paraffin-embedded sections of human liver array using Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685) at ...read more
Simple Western lane view shows lysates of MBA‑MB‑468 human breast cancer cell line untreated (-) or treated (+) with 50 µM Pervanadate (PV) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for ...read more
Src phosphorylated at Y419 was detected in immersion fixed paraffin-embedded sections of human colon using Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685) at 15 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Src [p Tyr419] Antibody [Unconjugated] Summary

Immunogen
Phosphopeptide containing human Src Y419 site
Modification
p Tyr419
Specificity
Detects human Src family members when phosphorylated at sites corresponding to Y419 of human Src in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SRC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF2685 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Src [p Tyr419] Antibody [Unconjugated]

  • ASV
  • c-Src
  • EC 2.7.10
  • EC 2.7.10.2
  • pp60c-src
  • Rous sarcoma
  • RSVgp4
  • Src
  • tyrosine kinase pp60c-src
  • tyrosine-protein kinase SRC-1
  • v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
  • v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)

Background

Src and other Src family protein tyrosine kinases are proto-oncogenes that play key roles in regulating cell growth and differentiation. Src family activity is regulated by tyrosine phosphorylation at two sites with opposing effects. Phosphorylation at Y419 of human Src up-regulates kinase activity, while phosphorylation at Y530 inactivates Src.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Src Antibody (AF2685)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC - Frozen, IHC Paraffin-embedded, WB.


Filter By Application
IHC - Frozen
(1)
IHC Paraffin-embedded
(1)
WB
(3)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
All Species
Showing Publications 1 - 5 of 5.
Publications using AF2685 Applications Species
Y Mao, S Sun, KD Irvine Role and regulation of Yap in KrasG12D-induced lung cancer Oncotarget, 2017;8(67):110877-110889. 2017 [PMID: 29340023] (IHC - Frozen,, Mouse) IHC - Frozen Mouse
JH Pak, Q Bashir, IK Kim, SJ Hong, S Maeng, YY Bahk, TS Kim Clonorchis sinensis excretory-secretory products promote the migration and invasion of cholangiocarcinoma cells by activating the integrin ?4-FAK/Src signaling pathway Mol. Biochem. Parasitol, 2017;0(0):. 2017 [PMID: 28286026] (WB,, Human) WB Human
Cahoon J, Rai R, Carroll L, Uehara H, Zhang X, O'Neil C, Medina R, Das S, Muddana S, Olson P, Nielson S, Walker K, Flood M, Messenger W, Archer B, Barabas P, Krizaj D, Gibson C, Li D, Koh G, Gao G, Stitt A, Ambati B Intravitreal AAV2.COMP-Ang1 Prevents Neurovascular Degeneration in a Murine Model of Diabetic Retinopathy. Diabetes, 2015;64(12):4247-59. 2015 [PMID: 26340930] (WB,, Human) WB Human
Ye F, Zhou F, Nithianantham S, Chandran B, Yu X, Weinberg A, Gao S Kaposi's sarcoma-associated herpesvirus induces rapid release of angiopoietin-2 from endothelial cells. J Virol, 2013;87(11):6326-35. 2013 [PMID: 23536671] (WB,, Human) WB Human
Matsuo K, Nishimura M, Bottsford-Miller JN, Huang J, Komurov K, Armaiz-Pena GN, Shahzad MM, Stone RL, Roh JW, Sanguino AM, Lu C, Im DD, Rosenshien NB, Sakakibara A, Nagano T, Yamasaki M, Enomoto T, Kimura T, Ram PT, Schmeler KM, Gallick GE, Wong KK, Frumovitz M, Sood AK Targeting Src in Mucinous Ovarian Carcinoma. Clin. Cancer Res., 2011;17(16):5367-5378. 2011 [PMID: 21737505] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human

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Video Protocols

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Bioinformatics

Gene Symbol SRC
Entrez