Nrf2 Antibody

Images

 
Western Blot: Nrf2 Antibody [NBP1-32822] - Hydralazine enhances NRF2 signaling in SH-SY5Y cells. c Hydralazine reduced the interaction between NRF2 and KEAP1. Interactions were measured by reciprocal Co-IPs followed by ...read more
Immunocytochemistry/ Immunofluorescence: Nrf2 Antibody [NBP1-32822] - NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at ...read more
Immunohistochemistry-Paraffin: Nrf2 Antibody [NBP1-32822] - NRF2 antibody [N2C2], Internal detects NRF2 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast ...read more
Chromatin Immunoprecipitation: Nrf2 Antibody [NBP1-32822] - ChIP was performed with HepG2 chromatin extract and 5 ug of either normal rabbit IgG or anti-NRF2 antibody. The precipitated DNA was detected by PCR with ...read more
Western Blot: Nrf2 Antibody [NBP1-32822] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at 1:500.
Western Blot: Nrf2 Antibody [NBP1-32822] - Non-transfected (-) and NRF2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 ...read more
Western Blot: Nrf2 Antibody [NBP1-32822] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at 1:500. The HRP-conjugated ...read more
Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) RAW264.7 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at ...read more
Western Blot: Nrf2 Antibody [NBP1-32822] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at 1:500. The HRP-conjugated ...read more
Orthogonal Strategies: Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) Rat-2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody ...read more
Orthogonal Strategies: Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) Neuro2A whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody ...read more
Orthogonal Strategies: Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody ...read more
Western Blot: Nrf2 Antibody [NBP1-32822] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:500. The ...read more
Orthogonal Strategies: Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) MDA-MB-231 nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody ...read more
Independent Antibodies: Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with NRF2 antibody ...read more
Western Blot: Nrf2 Antibody [NBP1-32822] - Hydralazine enhances NRF2 signaling in SH-SY5Y cells d NRF2 translocates to the nucleus with hydralazine treatment. Treated cells were subjected to cell fractionation and ...read more
Immunocytochemistry/ Immunofluorescence: Nrf2 Antibody [NBP1-32822] - NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal diluted at 1:500. ...read more
Immunoprecipitation: Nrf2 Antibody [NBP1-32822] - Immunoprecipitation of NRF2 protein from HepG2 whole cell extracts using 5 ug of NRF2 antibody [N2C2], Internal Western blot analysis was performed using NRF2 antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Al, Av, Bv, Ce, Pl, RMSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Nrf2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human NRF2. The exact sequence is proprietary.
Localization
Cytoplasm, cytosol, Nucleus
Predicted Species
Rhesus Macaque (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NFE2L2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Knockdown Validated
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Nrf2 Knockout HeLa Cell Lysate
Reviewed Applications
Read 4 Reviews rated 4
using
NBP1-32822 in the following applications:

Publications
Read Publications using
NBP1-32822 in the following applications:

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID:32756342). C. elegans reactivity reported in scientific literature (PMID: 29263362). Bovine reactivity reported in scientific literature (PMID:31068538).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for Nrf2 Antibody

  • HEBP1
  • NFE2L2
  • NF-E2-related factor 2
  • NFE2-related factor 2
  • NRF2 erythroid derived 2, like 2
  • Nrf2 ihc
  • Nrf2 immunofluorescence
  • Nrf2 immunohistochemistry
  • Nrf2 polyclonal
  • Nrf2 rat
  • Nrf2 western blot
  • Nrf2
  • nuclear factor (erythroid-derived 2)-like 2
  • nuclear factor erythroid 2-related factor 2
  • nuclear factor erythroid-derived 2-like 2

Background

Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor which belongs to the Cap N Collar (CNC-bZIP) subfamily with an implication in adaptive responses to oxidative stress. Nrf2 is a basic leucine zipper transcription factor that can bind to a NF-E2/AP-1 repeat sequence in the promoter of the beta-globin gene and has two types of binding partners, a cytoplasmic repressor (Keap I) and multiple nuclear binding partners. During oxidative stress on a cell, Nrf2 is released from Keap I, possibly via PKC phosphorylation at serine 40, and translocates to the nucleus. In the nucleus, Nrf2 binds to antioxidant responsive element (ARE) and leads to transcriptional activation of downstream genes for GSTs, glutamate-cysteine ligase, HO-1, and thioredoxin. Nrf2 and its downstream proteins have shown to have important functions in protection against oxidative stress or chemical-induced cellular damage in liver and lung, prevention in cancer formation in GI tract, and promotion of the wound-healing process.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-32822
Species: Hu, Mu, Rt, Al, Av, Bv, Ce, Pl, RM
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, KD

Publications for Nrf2 Antibody (NBP1-32822)(28)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Bovine.

We have publications tested in 6 applications: ChIP, IHC, IP, KD, Single-Cell Western, WB.


Filter By Application
ChIP
(1)
IHC
(1)
IP
(1)
KD
(1)
Single-Cell Western
(1)
WB
(17)
All Applications
Filter By Species
Human
(9)
Mouse
(10)
Rat
(1)
Bovine
(2)
All Species
Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using NBP1-32822 Applications Species
Mumford PW, Osburn SC, Fox CD et al. A Theacrine-Based Supplement Increases Cellular NAD+ Levels and Affects Biomarkers Related to Sirtuin Activity in C2C12 Muscle Cells In Vitro Nutrients Dec 3 2020 [PMID: 33287129] (WB, Mouse)

Details:
C2C12 cells underwent Western blot analysis.
WB Mouse
Wen J, Li S, Zheng C et al. Tetramethylpyrazine nitrone improves motor dysfunction and pathological manifestations by activating the PGC-1 alpha/Nrf2/HO-1 pathway in ALS mice Neuropharmacology Nov 1 2020 [PMID: 33152451] (WB, Mouse) WB Mouse
Chen HC, Yip T, Lee JK et al. Restraint Stress Alters Expression of Glucocorticoid Bioavailability Mediators, Suppresses Nrf2, and Promotes Oxidative Stress in Liver Tissue Antioxidants (Basel, Switzerland) Sep 11 2020 [PMID: 32932938] (Single-Cell Western, Mouse) Single-Cell Western Mouse
Hou X, Li Z, Higashi Y et al. Insulin-Like Growth Factor I Prevents Cellular Aging via Activation of Mitophagy J Aging Res Aug 1 2020 [PMID: 32802505] (WB) WB
Fusco R, Cordaro M, Siracusa R et al. Effects of Hydroxytyrosol against Lipopolysaccharide-Induced Inflammation and Oxidative Stress in Bovine Mammary Epithelial Cells: A Natural Therapeutic Tool for Bovine Mastitis Antioxidants (Basel) Aug 3 2020 [PMID: 32756342] (WB, Bovine) WB Bovine
Catanzaro M, Lanni C, Basagni F et al. Eye-Light on Age-Related Macular Degeneration: Targeting Nrf2-Pathway as a Novel Therapeutic Strategy for Retinal Pigment Epithelium Front Pharmacol Jun 5 2020 [PMID: 32581803]
Leung MH, Tsoi H, Gong C et al. A Splice Variant of NCOR2, BQ323636.1, Confers Chemoresistance in Breast Cancer by Altering the Activity of NRF2 Cancers (Basel) Feb 26 2020 [PMID: 32110852] (WB, Human) WB Human
Serafini M, Catanzaro M, Fagiani F et al. Modulation of Keap1/Nrf2/ARE Signaling Pathway by Curcuma- and Garlic-Derived Hybrids Front. Pharmacol. Jan 28 2020 [PMID: 32047434] (WB, Human) WB Human
Nagappan A, Kim JH, Jung DY, Jung MH Cryptotanshinone from the Salvia miltiorrhiza Bunge Attenuates Ethanol-Induced Liver Injury by Activation of AMPK/SIRT1 and Nrf2 Signaling Pathways Int J Mol Sci Dec 30 2019 [PMID: 31906014] (WB, Human) WB Human
Kaya O, Orhan E, Sapmaz-Metin M, et al. The effects of epidermal growth factor on early burn-wound progression in rats Dermatol Ther Dec 18 2019 [PMID: 31849151] (IHC, Rat) IHC Rat
Show All 28 Publications.

Reviews for Nrf2 Antibody (NBP1-32822) (4) 44

Average Rating: 4
(Based on 4 reviews)
We have 4 reviews tested in 3 species: Human, Mouse, rat skin wound and Rat.

Reviews using NBP1-32822:
Filter by Applications
Simple Western
(1)
WB
(3)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
rat skin wound and Rat
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western Nrf2 NBP1-32822
Enlarge
4
reviewed by:
Anonymous
Simple Western rat skin wound and Rat 02/07/2020
View

Summary

ApplicationSimple Western
Sample Testedskin wound
Speciesrat skin wound and Rat
Lot43552
Western Blot Nrf2 NBP1-32822
Enlarge
4
reviewed by:
YONGCHUL JANG
WB Mouse 10/05/2017
View

Summary

ApplicationWestern Blot
Sample TestedBrain (substantia nigra)
SpeciesMouse
LotNBP1-32822
  5
reviewed by:
Anonymous
WB Human 04/28/2017
View

Summary

ApplicationWestern Blot
Sample TestedBxPC3, GH3 and SH-SY5Y whole cell lysates
SpeciesHuman
Western Blot Nrf2 NBP1-32822
Enlarge
3
reviewed by:
Byron Baron
WB Human 03/01/2017
View

Summary

ApplicationWestern Blot
Sample TestedHepG2 (liver) cell lysate
SpeciesHuman
Lot42004

Comments

CommentsPrimary rabbit anti NRF2 antibody diluted 1:500 in TBS. Membrane incubation for 2 hours at RT. Secondary IRDye 800CW goat anti rabbit antibody diluted 1:10000 in TBS. Membrane incubation for 1 hour at RT. Detection by Li-Cor Odyssey

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Nrf2 Antibody (NBP1-32822). (Showing 1 - 1 of 1 FAQs).

  1. Would you please check if thimerosal is contained or not contained in the current lot of this item?
    • I can confirm that our Nrf2 antibody with catalogue number NBP1-32822 is supplied in Phosphate Buffered Saline (PBS) with 20% glycerol and 0.025% Proclin. The change to the preservative was made at the beginning of April 2015.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Blogs on Nrf2.

Autophagic flux: Is p62 a good indicator?
By Christina Towers, PhD Is p62 a good indicator of autophagic flux? The short answer: Yes … but … SQSTM1 encodes the cargo adaptor protein, p62, which interacts with autophagic substrates and delivers them to aut...  Read full blog post.

Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease
 By Jamshed Arslan Pharm.D.  Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amy...  Read full blog post.

Crosstalk Between Oxidative Stress and Autophagy
By Christina Towers, PhD. Role of Reactive Species in Cellular FunctionOxidative stress is a byproduct of an imbalance between oxidants and antioxidants present in the cell, resulting in dysfunctional redox si...  Read full blog post.

PGC-1 alpha: Roles in Mitochondrial Biogenesis and Disease
An important aspect of mitochondria maintenance includes biogenesis to replenish damaged and degraded mitochondrial structures. The regulation of mitochondrial biogenesis is very complex and numerous genes regulate and synchronize protein synthesis fr...  Read full blog post.

Exploring the Many Roles of PGC-1 alpha
The peroxisome proliferator-activated receptor gamma, co-activator 1 (PGC-1 alpha or PPARGC1A) gene encodes a 91 kDa nuclear protein that acts as a transcriptional co-activator involved in energy metabolism. Interaction with PPAR gamma allows it to in...  Read full blog post.

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Recent Reviews

4
5
1
4
2
3
1
2
0
1
0

Anonymous
02/07/2020
Application: Simple Western
Species: rat skin wound and Rat

YONGCHUL JANG
10/05/2017
Application: WB
Species: Mouse

 
Anonymous
04/28/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol NFE2L2
Entrez
Uniprot