Nrf2 Antibody

Western Blot: Nrf2 Antibody [NBP1-32822] - Hydralazine enhances NRF2 signaling in SH-SY5Y cells. c Hydralazine reduced the interaction between NRF2 and KEAP1. Interactions were measured by reciprocal Co-IPs followed by western blot analysis. *p < 0.05, two-tailed Student's t test, n = 3, mean +/- SD. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-02394-3), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Nrf2 Antibody [NBP1-32822] - NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NRF2 stained by NRF2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

Immunohistochemistry-Paraffin: Nrf2 Antibody [NBP1-32822] - NRF2 antibody [N2C2], Internal detects NRF2 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.NRF2 stained by NRF2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Chromatin Immunoprecipitation: Nrf2 Antibody [NBP1-32822] - ChIP was performed with HepG2 chromatin extract and 5 ug of either normal rabbit IgG or anti-NRF2 antibody. The precipitated DNA was detected by PCR with primer set targeting to GCLC gene locus.

Western Blot: Nrf2 Antibody [NBP1-32822] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at 1:500.

Western Blot: Nrf2 Antibody [NBP1-32822] - Non-transfected (-) and NRF2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody diluted by 1:1000.

Western Blot: Nrf2 Antibody [NBP1-32822] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) RAW264.7 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Nrf2 Antibody [NBP1-32822] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) Rat-2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) Neuro2A whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Nrf2 Antibody [NBP1-32822] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) MDA-MB-231 nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:1000.

Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with NRF2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Nrf2 Antibody [NBP1-32822] - Hydralazine enhances NRF2 signaling in SH-SY5Y cells d NRF2 translocates to the nucleus with hydralazine treatment. Treated cells were subjected to cell fractionation and western blot analysis. *p < 0.05 and **p < 0.01, two-tailed Student's t test, n = 3, mean +/- SD. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-02394-3), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Nrf2 Antibody [NBP1-32822] - NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunoprecipitation: Nrf2 Antibody [NBP1-32822] - Immunoprecipitation of NRF2 protein from HepG2 whole cell extracts using 5 ug of NRF2 antibody [N2C2], Internal Western blot analysis was performed using NRF2 antibody [N2C2], Internal.. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt, Al, Av, Pl, Ze, RM
• Applications: WB, Simple Western, Flow, ICC/IF, IHC, IP, ChIP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Nrf2 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human NRF2. The exact sequence is proprietary.
• Localization: Cytoplasm, cytosol, Nucleus
• Predicted Species: Rhesus Macaque (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NFE2L2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) -Assay dependent
  • Flow Cytometry -Assay dependent
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Knockdown Validated
  • Simple Western -Reported by internal validation
  • Western Blot 1:500-1:3000
• Application Notes: In Simple Western internal validation: Rat skin wound at 0.5 mg/ml as sample; separated by size; antibody dilution of 1:20 - 1:500; observed molecular weight was 78 kDa; detected by Chemiluminescence.
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for Nrf2 Antibody

• HEBP1
• NFE2L2
• NF-E2-related factor 2
• NFE2-related factor 2
• NRF2 erythroid derived 2, like 2
• Nrf2 ihc
• Nrf2 immunofluorescence
• Nrf2 immunohistochemistry
• Nrf2 polyclonal
• Nrf2 rat
• Nrf2 western blot
• Nrf2
• nuclear factor (erythroid-derived 2)-like 2
• nuclear factor erythroid 2-related factor 2
• nuclear factor erythroid-derived 2-like 2

Background

Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor which belongs to the Cap N Collar (CNC-bZIP) subfamily with an implication in adaptive responses to oxidative stress. Nrf2 is a basic leucine zipper transcription factor that can bind to a NF-E2/AP-1 repeat sequence in the promoter of the beta-globin gene and has two types of binding partners, a cytoplasmic repressor (Keap I) and multiple nuclear binding partners. During oxidative stress on a cell, Nrf2 is released from Keap I, possibly via PKC phosphorylation at serine 40, and translocates to the nucleus. In the nucleus, Nrf2 binds to antioxidant responsive element (ARE) and leads to transcriptional activation of downstream genes for GSTs, glutamate-cysteine ligase, HO-1, and thioredoxin. Nrf2 and its downstream proteins have shown to have important functions in protection against oxidative stress or chemical-induced cellular damage in liver and lung, prevention in cancer formation in GI tract, and promotion of the wound-healing process.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nrf2 Antibody (NBP1-32822)(51)

Publications using NBP1-32822

• Yan B, Mao X, Hu S et al. Spermidine protects intestinal mucosal barrier function in mice colitis via the AhR/Nrf2 and AhR/STAT3 signaling pathways International immunopharmacology 2023-04-25 [PMID: 37104918] (WB, Mouse)
  
  Details:
  1:1000 WB dilution
• Urzì O, Cafora M, Ganji N et al. Lemon-derived nanovesicles achieve antioxidant and anti-inflammatory effects activating the AhR/Nrf2 signaling pathway iScience 2023-07-01 [PMID: 37426343] (WB, Human)
• Milara J, MartInez-ExpOsito F, Montero P et al. N-acetylcysteine Reduces Inflammasome Activation Induced by SARS-CoV-2 Proteins In Vitro International Journal of Molecular Sciences 2022-11-22 [PMID: 36498845] (IHC-P, Human)
  
  Details:
  differentiated 937 macrophages were stimulated
• Meister ML, Najjar RS, Danh JP et al. Berry consumption mitigates the hypertensive effects of a high-fat, high-sucrose diet via attenuation of renal and aortic AT1R expression resulting in improved endothelium-derived NO bioavailability The Journal of nutritional biochemistry 2022-11-23 [PMID: 36435288] (WB, Human, Mouse)
• Vijayan M, Reddy PH Reduced VDAC1, Maintained Mitochondrial Dynamics and Enhanced Mitochondrial Biogenesis in a Transgenic Tau Mouse Model of Alzheimer's Disease International journal of molecular sciences 2022-08-02 [PMID: 35955694] (IF/IHC, WB, Mouse)
  
  Details:
  WB dilution used 1:1000, IHC dilution used 1:102
• Tabandeh MR, Jozaie S, Ghotbedin Z, Gorani S Dimethyl itaconic acid improves viability and steroidogenesis and suppresses cytokine production in LPS-treated bovine ovarian granulosa cells by regulating TLR4/nfk beta, NLRP3, JNK signaling pathways Research in veterinary science 2022-08-01 [PMID: 35933803] (WB, Bovine)
• Taheri M, Roudbari NH, Amidi F, Parivar K Investigating the effect of Sulforaphane on AMPK/AKT/NRF2 pathway in human granulosa-lutein cells under H2O2-induced oxidative stress European journal of obstetrics, gynecology, and reproductive biology 2022-07-14 [PMID: 35882072]
• Wolzak K, Nolle A, Farina M et al. Neuron-specific translational control shift ensures proteostatic resilience during ER stress The EMBO journal 2022-07-06 [PMID: 35791631] (ICC/IF, Human)
• Najjar R, Mu S, Feresin R Blueberry Polyphenols Increase Nitric Oxide and Attenuate Angiotensin II-Induced Oxidative Stress and Inflammatory Signaling in Human Aortic Endothelial Cells Antioxidants 2022-03-23 [PMID: 35453301] (WB, Human)
• Pratelli G, Carlisi D, D'Anneo A et al. Bio-Waste Products of Mangifera indica L. Reduce Adipogenesis and Exert Antioxidant Effects on 3T3-L1 Cells Antioxidants (Basel, Switzerland) 2022-02-11 [PMID: 35204243] (WB)
• Guerrero-Escalera D, AlarcOn-SAnchez BR, Arellanes-Robledo J et al. Comparative subcellular localization of NRF2 and KEAP1 during the hepatocellular carcinoma development in vivo Biochimica et biophysica acta. Molecular cell research 2022-01-27 [PMID: 35093454] (WB, Rat, Bovine)
• Pak ME, Oh YC, Park YJ et al. Banhasasim-Tang Ameliorates Spatial Memory by Suppressing Oxidative Stress through Regulation of ERK/p38 Signaling in Hippocampus of Mice Oxidative medicine and cellular longevity 2021-12-02 [PMID: 34900088] (WB, Mouse)
• Arigela CS, Nelli G, Gan SH Et al. Bitter Gourd Honey Ameliorates Hepatic and Renal Diabetic Complications on Type 2 Diabetes Rat Models by Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Mechanisms Foods (Basel, Switzerland) 2021-11-20 [PMID: 34829154] (ICC/IF, Rat)
• Kshirsagar S, Sawant N, Morton H Et al. Mitophagy enhancers against phosphorylated Tau-induced mitochondrial and synaptic toxicities in Alzheimer disease Pharmacological research 2021-11-08 [PMID: 34763094] (WB, Mouse)
• Taheri M, Hayati Roudbari N, Amidi F, Parivar K The protective effect of sulforaphane against oxidative stress in granulosa cells of patients with polycystic ovary syndrome (PCOS) through activation of AMPK/AKT/NRF2 signaling pathway Reproductive biology 2021-10-19 [PMID: 34678578] (WB, Human)
• Vendidandala NR, Yin TP, Nelli G Et al. Gallocatechin silver nanoparticle impregnated cotton gauze patches enhance wound healing in diabetic rats by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-kappa B pathways Life sciences 2021-10-06 [PMID: 34624322] (IHC-P, Rat)
• Jawad A, Yoo Yj, Yoon Jc Et Al. Changes of renal histopathology and the role of Nrf2/HO-1 in asphyxial cardiac arrest model in rats Acta cirurgica brasileira 2021-07-19 [PMID: 34287609] (IHC-P)
• Jing M, Cen Y, Gao F Et Al. Nephroprotective Effects of Tetramethylpyrazine Nitrone TBN in Diabetic Kidney Disease Frontiers in pharmacology 2021-06-24 [PMID: 34248629] (WB)
• Delaidelli A, Richner M, Jiang L et al. alpha-Synuclein pathology in Parkinson disease activates homeostatic NRF2 anti-oxidant response Acta neuropathologica communications 2021-06-06 [PMID: 34092244] (ICC/IF, Mouse)
• Huang Y, Zhang X Luteolin alleviates polycystic ovary syndrome in rats by resolving insulin resistance and oxidative stress American journal of physiology. Endocrinology and metabolism 2021-04-26 [PMID: 33900851]
• Oh Y, Li W, Choi J Saussureae Radix Attenuates Neuroinflammation in LPS-Stimulated Mouse BV2 Microglia via HO-1/Nrf-2 Induction and Inflammatory Pathway Inhibition Mediators of Inflammation 2021-03-18 [PMID: 33790692] (WB, Mouse)
• Kim S, Kang SW, Joo J et al. Characterization of ferroptosis in kidney tubular cell death under diabetic conditions Cell death & disease 2021-02-08 [PMID: 33558472] (WB)
• Mumford PW, Osburn SC, Fox CD et al. A Theacrine-Based Supplement Increases Cellular NAD+ Levels and Affects Biomarkers Related to Sirtuin Activity in C2C12 Muscle Cells In Vitro Nutrients 2020-12-03 [PMID: 33287129] (WB, Mouse)
  
  Details:
  C2C12 cells underwent Western blot analysis.
• Wen J, Li S, Zheng C et al. Tetramethylpyrazine nitrone improves motor dysfunction and pathological manifestations by activating the PGC-1 alpha/Nrf2/HO-1 pathway in ALS mice Neuropharmacology 2020-11-01 [PMID: 33152451] (WB, Mouse)
• Chen HC, Yip T, Lee JK et al. Restraint Stress Alters Expression of Glucocorticoid Bioavailability Mediators, Suppresses Nrf2, and Promotes Oxidative Stress in Liver Tissue Antioxidants (Basel, Switzerland) 2020-09-11 [PMID: 32932938] (Single-Cell Western, Mouse)
• Fagiani F, Catanzaro M, Buoso E Targeting Cytokine Release Through the Differential Modulation of Nrf2 and NF-kB Pathways by Electrophilic/Non-Electrophilic Compounds Front Pharmacol 2020-09-14 [PMID: 32922294]
• Fujie, T, Takahashi, A Et al. Transcriptional Induction of Cystathionine gamma-Lyase, a Reactive Sulfur-Producing Enzyme, by Copper Diethyldithiocarbamate in Cultured Vascular Endothelial Cells. Int J Mol Sci 2020-08-22 [PMID: 32842680] (WB, Rat)
• Hou X, Li Z, Higashi Y et al. Insulin-Like Growth Factor I Prevents Cellular Aging via Activation of Mitophagy J Aging Res 2020-08-01 [PMID: 32802505] (WB)
• Fusco R, Cordaro M, Siracusa R et al. Effects of Hydroxytyrosol against Lipopolysaccharide-Induced Inflammation and Oxidative Stress in Bovine Mammary Epithelial Cells: A Natural Therapeutic Tool for Bovine Mastitis Antioxidants (Basel) 2020-08-03 [PMID: 32756342] (WB, Bovine)
• Catanzaro M, Lanni C, Basagni F et al. Eye-Light on Age-Related Macular Degeneration: Targeting Nrf2-Pathway as a Novel Therapeutic Strategy for Retinal Pigment Epithelium Front Pharmacol 2020-06-05 [PMID: 32581803]
• Leung MH, Tsoi H, Gong C et al. A Splice Variant of NCOR2, BQ323636.1, Confers Chemoresistance in Breast Cancer by Altering the Activity of NRF2 Cancers (Basel) 2020-02-26 [PMID: 32110852] (WB, Human)
• Serafini M, Catanzaro M, Fagiani F et al. Modulation of Keap1/Nrf2/ARE Signaling Pathway by Curcuma- and Garlic-Derived Hybrids Front. Pharmacol. 2020-01-28 [PMID: 32047434] (WB, Human)
• Nagappan A, Kim JH, Jung DY, Jung MH Cryptotanshinone from the Salvia miltiorrhiza Bunge Attenuates Ethanol-Induced Liver Injury by Activation of AMPK/SIRT1 and Nrf2 Signaling Pathways Int J Mol Sci 2019-12-30 [PMID: 31906014] (WB, Human)
• Kaya O, Orhan E, Sapmaz-Metin M, et al. The effects of epidermal growth factor on early burn-wound progression in rats Dermatol Ther 2019-12-18 [PMID: 31849151] (IF/IHC, Rat)
• Dehghan E, Goodarzi M, Saremi B et al. Hydralazine targets cAMP-dependent protein kinase leading to sirtuin1/5 activation and lifespan extension in C. elegans. Nat Commun. 2019-10-28 [PMID: 31659167] (WB, Human, Mouse)
• Kulabas SS, Onder FC, Yilmaz YB et al. In vitro and in silico studies of nitrobenzamide derivatives as potential anti-neuroinflammatory agents J. Biomol. Struct. Dyn. 2019-10-23 [PMID: 31642383]
• Sivandzade F, Cucullo L Anti-Diabetic Countermeasures Against Tobacco Smoke-Dependent Cerebrovascular Toxicity: Use and Effect of Rosiglitazone Int J Mol Sci [PMID: 31470514]
• Kovacs P, Csonka T, Kovacs T et al. Lithocholic Acid, a Metabolite of the Microbiome, Increases Oxidative Stress in Breast Cancer Cancers (Basel) 2019-08-27 [PMID: 31461945] (WB, Mouse, Human)
• Shao Y, Chen J, Dong LJ et al. A Protective Effect of PPAR alpha in Endothelial Progenitor Cells Through Regulating Metabolism Diabetes 2019-08-26 [PMID: 31451517] (WB, Mouse)
• Han YE, Kwon J, Won J et al. Possible mechanisms underlying transcriptional induction of metallothionein isoforms by tris(pentafluorophenyl)stibane, tris(pentafluorophenyl)arsane, and tris(pentafluorophenyl)phosphane in cultured bovine aortic endothelial cells J Toxicol Sci 2019-05-10 [PMID: 31068538] (Chemotaxis, Bovine)
• Singh R, Chandrashekharappa S, Bodduluri SR et al. Enhancement of the gut barrier integrity by a microbial metabolite through the Nrf2 pathway Nat Commun 2019-01-09 [PMID: 30626868] (WB, Mouse)
• Choo J, Heo G, Kim SJ et al. Senescence marker protein 30 protects intestinal epithelial cells against inflammation-induced cell death by enhancing Nrf2 activity. Biochim Biophys Acta Mol Basis Dis 2018-09-26 [PMID: 30266650] (IP, Human)
• Manczak M, Kandimalla R, Yin X, Hemachandra Reddy P. Mitochondrial division inhibitor 1 reduces dynamin-related protein 1 and mitochondrial fission activity. Hum. Mol. Genet. 2018-09-19 [PMID: 30239719] (WB, Mouse)
• Tumer TB, Yilmaz B, OzleYen A et al. GR24, a synthetic analog of Strigolactones, alleviates inflammation and promotes Nrf2 cytoprotective response: In vitro and in silico evidences. Comput Biol Chem. 2018-07-18 [PMID: 30048925] (WB, Mouse)
• Rungratanawanich W, Abate G, Serafini MM et al. Characterization of the Antioxidant Effects of g-Oryzanol: Involvement of the Nrf2 Pathway Oxid Med Cell Longev 2018-03-14 [PMID: 29725495] (WB, Human)
• Dehghan E, Zhang Y, Saremi B et al. Hydralazine induces stress resistance and extends C. elegans lifespan by activating the NRF2/SKN-1 signalling pathway. Nat Commun. 2017-12-20 [PMID: 29263362] (WB, KD, Human)
• Ko JH, Yoon SO, Lee HJ, Oh JY. Rapamycin regulates macrophage activation by inhibiting NLRP3 inflammasome-p38 MAPK-NF?B pathways in autophagy- and p62-dependent manners Oncotarget 2017-06-20 [PMID: 28489580] (WB, Human)
• Chung E, Joiner HE, Skelton T et al. Maternal exercise upregulates mitochondrial gene expression and increases enzyme activity of fetal mouse hearts. Physiol Rep. 2017-03-01 [PMID: 28292876] (WB, Mouse)
• Li G, Zhang Z, Quan Q et al. Discovery, Synthesis, and Functional Characterization of a Novel Neuroprotective Natural Product from the Fruit of Alpinia oxyphylla for use in Parkinson's Disease Through LC/MS-Based Multivariate Data Analysis-Guided Fractionation. J. Proteome Res. 2016-06-01 [PMID: 27246451]
• Sullivan LB, Martinez-Garcia E, Nguyen H et al. The proto-oncometabolite fumarate binds glutathione to amplify ROS-dependent signaling. Mol Cell. 2013-07-25 [PMID: 23747014]

Reviews for Nrf2 Antibody (NBP1-32822) (5)

Average Rating: 4

(Based on 5 reviews)

We have 5 reviews tested in 4 species: Human, Mouse, Rat, Rat and rat skin wound.

reviewed by: Verified Customer WB Rat 01/26/2023

Summary

• Application: Western Blot
• Sample Tested: Rat Cardiomyocyte
• Species: Rat
• Lot: 43782

reviewed by: Lenka Uherková Simple Western Rat and rat skin wound 02/07/2020

Summary

• Application: Simple Western
• Sample Tested: skin wound
• Species: Rat and rat skin wound
• Lot: 43552

reviewed by: Verified Customer WB Mouse 10/05/2017

Summary

• Application: Western Blot
• Sample Tested: Brain (substantia nigra)
• Species: Mouse
• Lot: NBP1-32822

reviewed by: Amirmansoor Hakimi WB Human 04/28/2017

Summary

• Application: Western Blot
• Sample Tested: BxPC3, GH3 and SH-SY5Y whole cell lysates
• Species: Human

reviewed by: Verified Customer WB Human 03/01/2017

Summary

• Application: Western Blot
• Sample Tested: HepG2 (liver) cell lysate
• Species: Human
• Lot: 42004

Comments

• Comments: Primary rabbit anti NRF2 antibody diluted 1:500 in TBS. Membrane incubation for 2 hours at RT. Secondary IRDye 800CW goat anti rabbit antibody diluted 1:10000 in TBS. Membrane incubation for 1 hour at RT. Detection by Li-Cor Odyssey

FAQs for Nrf2 Antibody (NBP1-32822). (Showing 1 - 1 of 1 FAQs).

1. Would you please check if thimerosal is contained or not contained in the current lot of this item?
   • I can confirm that our Nrf2 antibody with catalogue number NBP1-32822 is supplied in Phosphate Buffered Saline (PBS) with 20% glycerol and 0.025% Proclin. The change to the preservative was made at the beginning of April 2015.

Bioinformatics

• Gene Symbol: NFE2L2
• Entrez:
  • 4780(Human)
  • 18024(Mouse)
• Uniprot:
  • Q16236()