Nrf2 Antibody


Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) MDA-MB-231 nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody diluted at 1:500.The observed more
Immunocytochemistry/ Immunofluorescence: Nrf2 Antibody [NBP1-32822] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal diluted at 1:500. Red: more
Immunohistochemistry-Paraffin: Nrf2 Antibody [NBP1-32822] - Paraffin-embedded Cal27 xenograft, using Nrf2 antibody at 1:500 dilution.
Chromatin Immunoprecipitation (ChIP): Nrf2 Antibody [NBP1-32822] - ChIP was performed with HepG2 chromatin extract and 5 ug of either normal rabbit IgG or anti-NRF2 antibody. The precipitated DNA was detected by PCR more
Western Blot: Nrf2 Antibody [NBP1-32822] - Sample (30 ug of whole cell lysate) A: NIH-3T3 7. 5% SDS PAGE, Nrf2 antibody diluted at 1:2000.
Western Blot: Nrf2 Antibody [NBP1-32822] - Sample (20 ug) A: Hela whole cell lysateB: Hela cytoplasm C: Hela nucleus 7. 5% SDS PAGE; Nrf2 antibody diluted at 1:1000.
Western Blot: Nrf2 Antibody [NBP1-32822] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: Nrf2 Antibody [NBP1-32822] - Paraformaldehyde-fixed HeLa, using Nrf2 antibody at 1:200 dilution.
Chromatin Immunoprecipitation: Nrf2 Antibody [NBP1-32822] - Sample: HeLa whole cell lysate/extract A. 5 ug preimmune rabbit IgG, B. 5 ug of NRF2 antibody. The precipitated DNA was detected by PCR with primer set more
Nrf2 Antibody [NBP1-32822] - Non-transfected (-) and NRF2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody diluted by more
Immunoprecipitation: Nrf2 Antibody [NBP1-32822] - Immunoprecipitation of NRF2 protein from HepG2 whole cell extracts using 5 ug of NRF2 antibody [N2C2], Internal Western blot analysis was performed using NRF2 antibody more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P, IP
0.51 mg/ml

Order Details

Nrf2 Antibody Summary

Recombinant fragment corresponding to a region within amino acids 108 and 413 of NRF2 (Uniprot ID#Q16236)
Cytoplasm, cytosol, Nucleus
Predicted Species
Monkey (99%)
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
1XPBS, 20% Glycerol (pH 7.0)
0.025% Proclin 300
0.51 mg/ml
Immunogen affinity purified


Application Notes
For IHC-P, use suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
68 kDa
Read Publications using NBP1-32822.

Reactivity Notes

Rhesus Monkey (99%). Predicted cross-reactivity based on sequence identity: Bovine (84%).

Alternate Names for Nrf2 Antibody

  • HEBP1
  • NFE2L2
  • NF-E2-related factor 2
  • NFE2-related factor 2
  • NRF2 erythroid derived 2, like 2
  • Nrf2 ihc
  • Nrf2 immunofluorescence
  • Nrf2 immunohistochemistry
  • Nrf2 polyclonal
  • Nrf2 rat
  • Nrf2 western blot
  • nuclear factor (erythroid-derived 2)-like 2
  • nuclear factor erythroid 2-related factor 2
  • nuclear factor erythroid-derived 2-like 2


Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor which belongs to the Cap N Collar (CNC-bZIP) subfamily with an implication in adaptive responses to oxidative stress. Nrf2 is a basic leucine zipper transcription factor that can bind to a NF-E2/AP-1 repeat sequence in the promoter of the beta-globin gene and has two types of binding partners, a cytoplasmic repressor (Keap I) and multiple nuclear binding partners. During oxidative stress on a cell, Nrf2 is released from Keap I, possibly via PKC phosphorylation at serine 40, and translocates to the nucleus. In the nucleus, Nrf2 binds to antioxidant responsive element (ARE) and leads to transcriptional activation of downstream genes for GSTs, glutamate-cysteine ligase, HO-1, and thioredoxin. Nrf2 and its downstream proteins have shown to have important functions in protection against oxidative stress or chemical-induced cellular damage in liver and lung, prevention in cancer formation in GI tract, and promotion of the wound-healing process.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nrf2 Antibody (NBP1-32822)(2)

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Nrf2 Antibody (NBP1-32822). (Showing 1 - 1 of 1 FAQs).

  1. Would you please check if thimerosal is contained or not contained in the current lot of this item?
    • I can confirm that our Nrf2 antibody with catalogue number NBP1-32822 is supplied in Phosphate Buffered Saline (PBS) with 20% glycerol and 0.025% Proclin. The change to the preservative was made at the beginning of April 2015.

Secondary Antibodies


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Blogs on Nrf2.

PGC-1 alpha: Roles in Mitochondrial Biogenesis and Disease
An important aspect of mitochondria maintenance includes biogenesis to replenish damaged and degraded mitochondrial structures. The regulation of mitochondrial biogenesis is very complex and numerous genes regulate and synchronize protein synthesis fr...  Read full blog post.

Exploring the Many Roles of PGC-1 alpha
The peroxisome proliferator-activated receptor gamma, co-activator 1 (PGC-1 alpha or PPARGC1A) gene encodes a 91 kDa nuclear protein that acts as a transcriptional co-activator involved in energy metabolism. Interaction with PPAR gamma allows it to in...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol NFE2L2

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