Nrf2 Antibody

Images

 
Western Blot: Nrf2 Antibody [NBP1-32822] - Non-transfected (-) and NRF2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 ...read more
Immunocytochemistry/ Immunofluorescence: Nrf2 Antibody [NBP1-32822] - NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal diluted at 1:500. ...read more
Immunohistochemistry-Paraffin: Nrf2 Antibody [NBP1-32822] - Paraffin-embedded Cal27 xenograft, using Nrf2 antibody at 1:500 dilution.
Chromatin Immunoprecipitation (ChIP): Nrf2 Antibody [NBP1-32822] - ChIP was performed with HepG2 chromatin extract and 5 ug of either normal rabbit IgG or anti-NRF2 antibody. The precipitated DNA was detected by PCR ...read more
Western Blot: Nrf2 Antibody [NBP1-32822] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at 1:500.
Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) MDA-MB-231 nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody. The observed M.W. is based ...read more
Immunocytochemistry/ Immunofluorescence: Nrf2 Antibody [NBP1-32822] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal diluted at 1:500. Red: ...read more
Immunoprecipitation: Nrf2 Antibody [NBP1-32822] - Immunoprecipitation of NRF2 protein from HepG2 whole cell extracts using 5 ug of NRF2 antibody [N2C2], Internal Western blot analysis was performed using NRF2 antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, MkSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.51 mg/ml
   

Biological Strategies

     

Order Details

Nrf2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human NRF2. The exact sequence is proprietary.
Localization
Cytoplasm, cytosol, Nucleus
Predicted Species
Monkey (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NFE2L2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 4
using
NBP1-32822 in the following applications:

Publications
Read Publications using
NBP1-32822 in the following applications:

  • WB
    1 publication

Reactivity Notes

Rhesus Monkey (99%), Bovine (84%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
0.51 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Nrf2 Antibody

  • HEBP1
  • NFE2L2
  • NF-E2-related factor 2
  • NFE2-related factor 2
  • NRF2 erythroid derived 2, like 2
  • Nrf2 ihc
  • Nrf2 immunofluorescence
  • Nrf2 immunohistochemistry
  • Nrf2 polyclonal
  • Nrf2 rat
  • Nrf2 western blot
  • Nrf2
  • nuclear factor (erythroid-derived 2)-like 2
  • nuclear factor erythroid 2-related factor 2
  • nuclear factor erythroid-derived 2-like 2

Background

Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor which belongs to the Cap N Collar (CNC-bZIP) subfamily with an implication in adaptive responses to oxidative stress. Nrf2 is a basic leucine zipper transcription factor that can bind to a NF-E2/AP-1 repeat sequence in the promoter of the beta-globin gene and has two types of binding partners, a cytoplasmic repressor (Keap I) and multiple nuclear binding partners. During oxidative stress on a cell, Nrf2 is released from Keap I, possibly via PKC phosphorylation at serine 40, and translocates to the nucleus. In the nucleus, Nrf2 binds to antioxidant responsive element (ARE) and leads to transcriptional activation of downstream genes for GSTs, glutamate-cysteine ligase, HO-1, and thioredoxin. Nrf2 and its downstream proteins have shown to have important functions in protection against oxidative stress or chemical-induced cellular damage in liver and lung, prevention in cancer formation in GI tract, and promotion of the wound-healing process.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Mk
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP

Publications for Nrf2 Antibody (NBP1-32822)(3)

Reviews for Nrf2 Antibody (NBP1-32822) (3) 43

Average Rating: 4
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.
We have 3 reviews tested in 1 application: WB.

Reviews using NBP1-32822:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Nrf2 NBP1-32822
Enlarge
reviewed by:
YONGCHUL JANG
WB Mouse 10/05/2017
View

Summary

ApplicationWestern Blot
Sample TestedBrain (substantia nigra)
SpeciesMouse
LotNBP1-32822
 
reviewed by:
Anonymous
WB Human 04/28/2017
View

Summary

ApplicationWestern Blot
Sample TestedBxPC3, GH3 and SH-SY5Y whole cell lysates
SpeciesHuman
Western Blot Nrf2 NBP1-32822
Enlarge
reviewed by:
Byron Baron
WB Human 03/01/2017
View

Summary

ApplicationWestern Blot
Sample TestedHepG2 (liver) cell lysate
SpeciesHuman
Lot42004

Comments

CommentsPrimary rabbit anti NRF2 antibody diluted 1:500 in TBS. Membrane incubation for 2 hours at RT. Secondary IRDye 800CW goat anti rabbit antibody diluted 1:10000 in TBS. Membrane incubation for 1 hour at RT. Detection by Li-Cor Odyssey

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Nrf2 Antibody (NBP1-32822). (Showing 1 - 1 of 1 FAQs).

  1. Would you please check if thimerosal is contained or not contained in the current lot of this item?
    • I can confirm that our Nrf2 antibody with catalogue number NBP1-32822 is supplied in Phosphate Buffered Saline (PBS) with 20% glycerol and 0.025% Proclin. The change to the preservative was made at the beginning of April 2015.

Secondary Antibodies

 

Isotype Controls

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Blogs on Nrf2.

PGC-1 alpha: Roles in Mitochondrial Biogenesis and Disease
An important aspect of mitochondria maintenance includes biogenesis to replenish damaged and degraded mitochondrial structures. The regulation of mitochondrial biogenesis is very complex and numerous genes regulate and synchronize protein synthesis fr...  Read full blog post.

Exploring the Many Roles of PGC-1 alpha
The peroxisome proliferator-activated receptor gamma, co-activator 1 (PGC-1 alpha or PPARGC1A) gene encodes a 91 kDa nuclear protein that acts as a transcriptional co-activator involved in energy metabolism. Interaction with PPAR gamma allows it to in...  Read full blog post.

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Recent Reviews

4
5
1
4
1
3
1
2
0
1
0

YONGCHUL JANG
10/05/2017
Application: WB
Species: Mouse

 
Anonymous
04/28/2017
Application: WB
Species: Human

Byron Baron
03/01/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol NFE2L2
Entrez
Uniprot