Nrf2 Antibody

Images

 
Western Blot: Nrf2 Antibody [NBP1-32822] - Hydralazine enhances NRF2 signaling in SH-SY5Y cells. c Hydralazine reduced the interaction between NRF2 and KEAP1. Interactions were measured by reciprocal Co-IPs followed by ...read more
Immunocytochemistry/ Immunofluorescence: Nrf2 Antibody [NBP1-32822] - NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at ...read more
Immunohistochemistry-Paraffin: Nrf2 Antibody [NBP1-32822] - NRF2 antibody [N2C2], Internal detects NRF2 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast ...read more
Chromatin Immunoprecipitation: Nrf2 Antibody [NBP1-32822] - ChIP was performed with HepG2 chromatin extract and 5 ug of either normal rabbit IgG or anti-NRF2 antibody. The precipitated DNA was detected by PCR with ...read more
Western Blot: Nrf2 Antibody [NBP1-32822] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at 1:500.
Western Blot: Nrf2 Antibody [NBP1-32822] - Non-transfected (-) and NRF2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 ...read more
Western Blot: Nrf2 Antibody [NBP1-32822] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at 1:500. The HRP-conjugated ...read more
Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) RAW264.7 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at ...read more
Western Blot: Nrf2 Antibody [NBP1-32822] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at 1:500. The HRP-conjugated ...read more
Orthogonal Strategies: Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) Rat-2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody ...read more
Orthogonal Strategies: Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) Neuro2A whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody ...read more
Orthogonal Strategies: Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody ...read more
Western Blot: Nrf2 Antibody [NBP1-32822] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:500. The ...read more
Orthogonal Strategies: Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) MDA-MB-231 nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody ...read more
Independent Antibodies: Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with NRF2 antibody ...read more
Western Blot: Nrf2 Antibody [NBP1-32822] - Hydralazine enhances NRF2 signaling in SH-SY5Y cells d NRF2 translocates to the nucleus with hydralazine treatment. Treated cells were subjected to cell fractionation and ...read more
Immunocytochemistry/ Immunofluorescence: Nrf2 Antibody [NBP1-32822] - NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal diluted at 1:500. ...read more
Immunoprecipitation: Nrf2 Antibody [NBP1-32822] - Immunoprecipitation of NRF2 protein from HepG2 whole cell extracts using 5 ug of NRF2 antibody [N2C2], Internal Western blot analysis was performed using NRF2 antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Al, Av, Pl, Ze, RMSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Nrf2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human NRF2. The exact sequence is proprietary.
Localization
Cytoplasm, cytosol, Nucleus
Predicted Species
Rhesus Macaque (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NFE2L2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Knockdown Validated
  • Western Blot 1:500-1:3000
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-32822 in the following applications:

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33790692). Bovine (84%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for Nrf2 Antibody

  • HEBP1
  • NFE2L2
  • NF-E2-related factor 2
  • NFE2-related factor 2
  • NRF2 erythroid derived 2, like 2
  • Nrf2 ihc
  • Nrf2 immunofluorescence
  • Nrf2 immunohistochemistry
  • Nrf2 polyclonal
  • Nrf2 rat
  • Nrf2 western blot
  • Nrf2
  • nuclear factor (erythroid-derived 2)-like 2
  • nuclear factor erythroid 2-related factor 2
  • nuclear factor erythroid-derived 2-like 2

Background

Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor which belongs to the Cap N Collar (CNC-bZIP) subfamily with an implication in adaptive responses to oxidative stress. Nrf2 is a basic leucine zipper transcription factor that can bind to a NF-E2/AP-1 repeat sequence in the promoter of the beta-globin gene and has two types of binding partners, a cytoplasmic repressor (Keap I) and multiple nuclear binding partners. During oxidative stress on a cell, Nrf2 is released from Keap I, possibly via PKC phosphorylation at serine 40, and translocates to the nucleus. In the nucleus, Nrf2 binds to antioxidant responsive element (ARE) and leads to transcriptional activation of downstream genes for GSTs, glutamate-cysteine ligase, HO-1, and thioredoxin. Nrf2 and its downstream proteins have shown to have important functions in protection against oxidative stress or chemical-induced cellular damage in liver and lung, prevention in cancer formation in GI tract, and promotion of the wound-healing process.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-97507
Species: Bv, Ca, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
NBP2-03319
Species: Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB200-209
Species: Ca, Hu, Mu, Pm, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
NBP2-16017
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-47842
Species: Ca, Hu, Pm, Mu, Rt
Applications: IHC, IHC-P, IP, WB
H00002729-M01
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, WB
210-TA/CF
Species: Hu
Applications: BA
NBP2-67471
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NBP1-82580
Species: Hu
Applications: IHC, IHC-P, Simple Western, WB
AF3398
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
NB100-128
Species: Hu, Mu, Rt
Applications: GS, ICC/IF, IHC, IHC-P
D6050
Species: Hu
Applications: ELISA
NBP2-29323
Species: Bv, Ca, Ch, Hu, Mu, Rt, Xp
Applications: Func-Inh
NBP1-89125
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
AF467
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
NBP1-32822
Species: Hu, Mu, Rt, Al, Av, Pl, Ze, RM
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, KD

Publications for Nrf2 Antibody (NBP1-32822)(41)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Bovine.

We have publications tested in 8 applications: ChIP, ICC/IF, IHC, IHC-P, IP, KD, Single-Cell Western, WB.


Filter By Application
ChIP
(1)
ICC/IF
(2)
IHC
(1)
IHC-P
(2)
IP
(1)
KD
(1)
Single-Cell Western
(1)
WB
(25)
All Applications
Filter By Species
Human
(10)
Mouse
(14)
Rat
(5)
Bovine
(3)
All Species
Showing Publications 1 - 10 of 41. Show All 41 Publications.
Publications using NBP1-32822 Applications Species
Guerrero-Escalera D, AlarcOn-SAnchez BR, Arellanes-Robledo J et al. Comparative subcellular localization of NRF2 and KEAP1 during the hepatocellular carcinoma development in vivo Biochimica et biophysica acta. Molecular cell research Jan 27 2022 [PMID: 35093454] (WB, Rat, Bovine) WB Rat, Bovine
Pak ME, Oh YC, Park YJ et al. Banhasasim-Tang Ameliorates Spatial Memory by Suppressing Oxidative Stress through Regulation of ERK/p38 Signaling in Hippocampus of Mice Oxidative medicine and cellular longevity Dec 2 2021 [PMID: 34900088] (WB, Mouse) WB Mouse
Jawad A, Yoo Yj, Yoon Jc Et Al. Changes of renal histopathology and the role of Nrf2/HO-1 in asphyxial cardiac arrest model in rats Acta cirurgica brasileira Jul 19 2021 [PMID: 34287609] (IHC-P) IHC-P
Fagiani F, Catanzaro M, Buoso E Targeting Cytokine Release Through the Differential Modulation of Nrf2 and NF-kB Pathways by Electrophilic/Non-Electrophilic Compounds Frontiers in Pharmacology Jan 1 2020
Arigela CS, Nelli G, Gan SH Et al. Bitter Gourd Honey Ameliorates Hepatic and Renal Diabetic Complications on Type 2 Diabetes Rat Models by Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Mechanisms Foods (Basel, Switzerland) Nov 20 2021 [PMID: 34829154] (ICC/IF, Rat) ICC/IF Rat
Kshirsagar S, Sawant N, Morton H Et al. Mitophagy enhancers against phosphorylated Tau-induced mitochondrial and synaptic toxicities in Alzheimer disease Pharmacological research Nov 8 2021 [PMID: 34763094] (WB, Mouse) WB Mouse
Taheri M, Hayati Roudbari N, Amidi F, Parivar K The protective effect of sulforaphane against oxidative stress in granulosa cells of patients with polycystic ovary syndrome (PCOS) through activation of AMPK/AKT/NRF2 signaling pathway Reproductive biology Oct 19 2021 [PMID: 34678578] (WB, Human) WB Human
Vendidandala NR, Yin TP, Nelli G Et al. Gallocatechin silver nanoparticle impregnated cotton gauze patches enhance wound healing in diabetic rats by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-Kappa B pathways Life sciences Oct 6 2021 [PMID: 34624322] (IHC-P, Rat) IHC-P Rat
Jing M, Cen Y, Gao F Et Al. Nephroprotective Effects of Tetramethylpyrazine Nitrone TBN in Diabetic Kidney Disease Frontiers in pharmacology Jun 24 2021 [PMID: 34248629] (WB) WB
Delaidelli A, Richner M, Jiang L et al. alpha-Synuclein pathology in Parkinson disease activates homeostatic NRF2 anti-oxidant response Acta neuropathologica communications Jun 6 2021 [PMID: 34092244] (ICC/IF, Mouse) ICC/IF Mouse
Show All 41 Publications.

Reviews for Nrf2 Antibody (NBP1-32822) (0)

There are no reviews for Nrf2 Antibody (NBP1-32822). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for Nrf2 Antibody (NBP1-32822). (Showing 1 - 1 of 1 FAQs).

  1. Would you please check if thimerosal is contained or not contained in the current lot of this item?
    • I can confirm that our Nrf2 antibody with catalogue number NBP1-32822 is supplied in Phosphate Buffered Saline (PBS) with 20% glycerol and 0.025% Proclin. The change to the preservative was made at the beginning of April 2015.

Secondary Antibodies

 

Isotype Controls

Additional Nrf2 Products

Bioinformatics Tool for Nrf2 Antibody (NBP1-32822)

Discover related pathways, diseases and genes to Nrf2 Antibody (NBP1-32822). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Nrf2 Antibody (NBP1-32822)

Discover more about diseases related to Nrf2 Antibody (NBP1-32822).
 

Pathways for Nrf2 Antibody (NBP1-32822)

View related products by pathway.

PTMs for Nrf2 Antibody (NBP1-32822)

Learn more about PTMs related to Nrf2 Antibody (NBP1-32822).
 

Research Areas for Nrf2 Antibody (NBP1-32822)

Find related products by research area.

Blogs on Nrf2.

Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases
Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff...  Read full blog post.

Autophagic flux: Is p62 a good indicator?
By Christina Towers, PhD Is p62 a good indicator of autophagic flux? The short answer: Yes … but … SQSTM1 encodes the cargo adaptor protein, p62, which interacts with autophagic substrates and delivers them to aut...  Read full blog post.

Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease
 By Jamshed Arslan Pharm.D.  Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amy...  Read full blog post.

Crosstalk Between Oxidative Stress and Autophagy
By Christina Towers, PhD. Role of Reactive Species in Cellular FunctionOxidative stress is a byproduct of an imbalance between oxidants and antioxidants present in the cell, resulting in dysfunctional redox si...  Read full blog post.

PGC-1 alpha: Roles in Mitochondrial Biogenesis and Disease
An important aspect of mitochondria maintenance includes biogenesis to replenish damaged and degraded mitochondrial structures. The regulation of mitochondrial biogenesis is very complex and numerous genes regulate and synchronize protein synthesis fr...  Read full blog post.

Exploring the Many Roles of PGC-1 alpha
The peroxisome proliferator-activated receptor gamma, co-activator 1 (PGC-1 alpha or PPARGC1A) gene encodes a 91 kDa nuclear protein that acts as a transcriptional co-activator involved in energy metabolism. Interaction with PPAR gamma allows it to in...  Read full blog post.

Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Nrf2 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol NFE2L2
Entrez
Uniprot