RANK/TNFRSF11A Antibody (9A725) - BSA Free


Western Blot: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Analysis of RANK in transfected 293 cells using RANK antibody at 2 ug/ml.
Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) - BSA Free [NB100-56508] - An intracellular stain was performed on Raw264.7 cells with RANK/TNFRSF11A Antibody (9A725) NB100-56508 (blue) and a matched isotype control ...read more
Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Intracellular flow cytometry analysis of RANK in 10^6 RAW cells using 2 ug of RANK antibody. Shaded histogram represents RAW cells without antibody; green ...read more
Flow (Cell Surface): RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Analysis using the PE conjugate of NB100-56508. Staining of RANK in RAW cells using 0.5 ug of RANK antibody. Green histogram represents isotype ...read more
Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Using the PE direct conjugate An intracellular stain was performed on K-562 cells with RANK/TNFRSF11A (9A725) antibody NB100-56029 (blue) and a matched ...read more
Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - An intracellular stain was performed on Raw264.7 cells with RANK/TNFRSF11A [9A725] Antibody NB100-56508AF647 (blue) and a matched isotype control (orange). ...read more
Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) - BSA Free [NB100-56508] - An intracellular stain was performed on MG-63 cells with RANK/TNFRSF11A Antibody (9A725) NB100-56508 (blue) and a matched isotype control MAB002 ...read more
NDRG1 deficiency leads to abnormal bone formation caused by the impaired ability of macrophage lineage cells to differentiate into osteoclasts. (h) Expression of M-CSF receptor (M-CSFR) and RANKL receptor (RANK) in ...read more

Product Details

Reactivity Hu, Mu, Rt, AvSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
BSA Free
1.0 mg/ml

Order Details

RANK/TNFRSF11A Antibody (9A725) - BSA Free Summary

A fusion protein containing amino acid residues 326-616 of human RANK was used as immunogen. This antibody recognizes an epitope located between amino acid residues 330-427 of human RANK (NP_003830).
IgG1 Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Flow (Cell Surface) reported in scientific literature (PMID 24376119)
  • Flow Cytometry 2-4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1-2 ug/ml
Application Notes
Bharti et al (2004) published the RANK antibody clone 9A725 for Flow (Cell Surface). However, the epitope range recognized by this antibody is a potential cytoplasmic region as defined by www.uniprot.org. Hence, researchers are encouraged to refer to both the publication and the bioinformatics data bases for additional information in order to make their own determination regarding the suitability of the antibody for cell surface flow cytometry. In RAW cells, a 97 kDa is observed. This antibody is CyTOF ready.
Read Publications using
NB100-56508 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24376119). Avian reactivity reported in scientific literature (PMID: 31214852).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for RANK/TNFRSF11A Antibody (9A725) - BSA Free

  • CD265 antigen
  • CD265
  • FEO
  • loss of heterozygosity, 18, chromosomal region 1
  • ODFR
  • OFE
  • OPTB7
  • Osteoclast differentiation factor receptor
  • PDB2
  • RANK
  • Receptor activator of NF-KB
  • receptor activator of nuclear factor-kappa B
  • tumor necrosis factor receptor superfamily member 11A
  • tumor necrosis factor receptor superfamily, member 11a, activator of NFKB
  • tumor necrosis factor receptor superfamily, member 11a, NFKB activator


Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Receptor activator of NF-kB (RANK) is a recently cloned member of the TNFR superfamily with no significant homology to other members of this family. RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival . The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6. Overexpression of RANK activates NF-kB and c-Jun-terminal kinase (JNK) pathways. Recent studies have shown that RANK interaction with TRAF6 activates NF-kB, whereas JNK activation is mediated through binding of RANK to TRAF2.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Publications for RANK/TNFRSF11A Antibody (NB100-56508)(18)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Avian.

We have publications tested in 4 applications: FLOW, Flow-CS, IHC-P, WB.

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Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NB100-56508 Applications Species
Dorchin-Ashkenazi H, Ginat-Koton R, Gabet Y et al. The Balance between Orthodontic Force and Radiation in the Jawbone: Microstructural, Histological, and Molecular Study in a Rat Model Biology (Basel) 2021-11-18 [PMID: 34827196] (IHC-P, Rat)

Citation using the HRP format of this antibody.
Ferguson J, Wilcock DJ, McEntegart S et al. Osteoblasts contribute to a protective niche that supports melanoma cell proliferation and survival Pigment Cell Melanoma Res 2019-07-19 [PMID: 31323160] (WB, Human) WB Human
Hiyama S, Yokoi M, Akagi Y et al. Osteoclastogenesis from bone marrow cells during estrogen-induced medullary bone formation in Japanese quails J. Mol. Histol. 2019-08-01 [PMID: 31214852] (Avian)

Nanke Y, Kobashigawa T, Yago T et al. RANK Expression and Osteoclastogenesis in Human Monocytes in Peripheral Blood from Rheumatoid Arthritis Patients. BioMed Research International 2016-09-01 [PMID: 27822475] (FLOW, Human) FLOW Human
Huynh N, VonMoss L, Smith D et al. Characterization of Regulatory Extracellular Vesicles from Osteoclasts. J Dent Res 2016-06-01 [PMID: 26908631]
Watari K, Shibata T, Nabeshima H et al. Impaired differentiation of macrophage lineage cells attenuates bone remodeling and inflammatory angiogenesis in Ndrg1 deficient mice. Sci Rep. 2016-01-18 [PMID: 26778110] (WB, Mouse)

RANK antibody used for WB on BM-derived macrophages (BMDMs) from WT and KO mice at a dilution of 1:1000 (Figure 3h).
WB Mouse
Kaneko YS, Ota A, Nakashima A et al. Lipopolysaccharide treatment arrests the cell cycle of BV-2 microglial cells in G1 phase and protects them from UV light-induced apoptosis. J Neural Transm. 2014-06-12 [PMID: 24919883] (WB, Mouse)

Fig 3: BV-2 immortalized mouse microglial cell line. Procaspase and active/cleaved caspase were observed at 32 kDa and 17 kDa respectively.
WB Mouse
Das S, Sepahi I, Duthie A et al. RANk receptor oligomerisation in the regulation of NFkB signalling. J. Mol. Endocrinol. 2014-05-23 [PMID: 24859969] (WB, Mouse)

HeLa cells transfected with mouse WT-RANkmyc, W434X-RANkmyc or G280X-RANkmyc constructs, Fig 3Bi. The specifically of RANk mAb was validated by WB with recombinant expressed mouse WT-RANkmyc (80 kDa) and W434X-RANkmyc (55 kDa). The RANk mAb did not detect
WB Mouse
Lafferty MK, Fantry L, Bryant J et al. Elevated suppressor of cytokine signaling-1 (SOCS-1): a mechanism for dysregulated osteoclastogenesis in HIV transgenic rats Pathog Dis 2013-12-09 [PMID: 24376119] (Flow-CS, Rat)

This citation used the PE version of this antibody.
Flow-CS Rat
Izawa T, Zou W, Chappel JC et al. c-Src links a RANK/avB3 integrin complex to the osteoclast cytoskeleton. Mol Cell Biol. 2012-07-01 [PMID: 22615494] (WB, Mouse)

RANk (IMG-128A). 1. WB: Mice Splenocytes, Figure 1, 2C, 2D, & 2E.
WB Mouse
Show All 18 Publications.

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FAQs for RANK/TNFRSF11A Antibody (NB100-56508). (Showing 1 - 3 of 3 FAQs).

  1. Will I be able to use your stain for histological detection of RANK in bone sections for rat?
    • This antibody has been validated in IHC in paraffin-embedded tissues and is suitable for use on mouse and human samples. The rat RANK is 91% similar to mouse RANK, so this antibody is predicted to cross-react.
  2. Would you be able to confirm that Raw 264.7 cells were used in the flow cytometry validation of NB100-56508 - RANK/TNFRSF11A Antibody (9A725)?
    • Yes the cells used were Raw 264.7 cells.
  3. My lab has purchased RANK (clone 9A725) for IHC optimization in breast TMA sections. Can you recommend appropriate positive and negative control tissues?
    • Human Protein Atlas: https://www.proteinatlas.org/ENSG00000141655-TNFRSF11A/tissue. I see that brain Cerebral cortex/Hippocampus/Lateral ventricle/Cerebellum all have very good expression. Moderate high expression will be Fallopian tube/Stomach/Duodenum/Small intestine. Moderate low expression that compares to breast will be Cervix/ uterine/Endometrium. Negative expression will be Ovary/Oral mucosa/Salivary gland/Esophagus. Please check the website for other choices.

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Gene Symbol TNFRSF11A