RANK/TNFRSF11A Antibody (9A725)

Images

 
Western Blot: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Analysis of RANK in transfected 293 cells using RANK antibody at 2 ug/ml.
Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Using the PE direct conjugate An intracellular stain was performed on K-562 cells with RANK/TNFRSF11A (9A725) antibody NB100-56029 (blue) and a matched ...read more
Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Intracellular flow cytometry analysis of RANK in 10^6 RAW cells using 2 ug of RANK antibody. Shaded histogram represents RAW cells without antibody; green ...read more
Flow (Cell Surface): RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Analysis using the PE conjugate of NB100-56508. Staining of RANK in RAW cells using 0.5 ug of RANK antibody. Green histogram represents isotype ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, Flow-CS, IHC, IHC-P, CyTOF-ready
Clone
9A725
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

RANK/TNFRSF11A Antibody (9A725) Summary

Immunogen
A fusion protein containing amino acid residues 326-616 of human RANK was used as immunogen. This antibody recognizes an epitope located between amino acid residues 330-427 of human RANK (NP_003830).
Specificity
In RAW cells, a 97 kDa is observed.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TNFRSF11A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Flow Cytometry 2-4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • CyTOF-ready
  • Flow (Cell Surface)
Application Notes
Bharti et al (2004) and Reigel et al (2012) both published the RANK antibody clone 9A725 for Flow (Cell Surface). However, the epitope range recognized by this antibody is a potential cytoplasmic region as defined by www.uniprot.org. Hence, researchers are encouraged to refer to both the publication and the bioinformatics data bases for additional information in order to make their own determination regarding the suitability of the antibody for cell surface flow cytometry. Use in Flow cell surface reported in scientific literature (PMID 24376119). This antibody is CyTOF ready.
Publications
Read Publications using
NB100-56508 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24376119). Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for RANK/TNFRSF11A Antibody (9A725)

  • CD265 antigen
  • CD265
  • FEO
  • loss of heterozygosity, 18, chromosomal region 1
  • ODFR
  • ODFROSTS
  • OFE
  • OPTB7
  • Osteoclast differentiation factor receptor
  • PDB2
  • RANK
  • RANKLOH18CR1
  • Receptor activator of NF-KB
  • receptor activator of nuclear factor-kappa B
  • TNFRSF11A
  • TRANCE R
  • TRANCER
  • tumor necrosis factor receptor superfamily member 11A
  • tumor necrosis factor receptor superfamily, member 11a, activator of NFKB
  • tumor necrosis factor receptor superfamily, member 11a, NFKB activator

Background

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Receptor activator of NF-kB (RANK) is a recently cloned member of the TNFR superfamily with no significant homology to other members of this family. RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival. The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6. Overexpression of RANK activates NF-kB and c-Jun-terminal kinase (JNK) pathways. Recent studies have shown that RANK interaction with TRAF6 activates NF-kB, whereas JNK activation is mediated through binding of RANK to TRAF2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, Flow-CS, IHC, IHC-P, CyTOF-ready
Supplier Logo

Publications for RANK/TNFRSF11A Antibody (NB100-56508)(16)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: FLOW, Flow-CS, WB.


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FLOW
(1)
Flow-CS
(2)
WB
(7)
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Filter By Species
Human
(2)
Mouse
(4)
Rat
(2)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB100-56508 Applications Species
Nanke Y, Kobashigawa T, Yago T et al. RANK Expression and Osteoclastogenesis in Human Monocytes in Peripheral Blood from Rheumatoid Arthritis Patients. BioMed Research International Sep 1 2016 [PMID: 27822475] (FLOW, Human) FLOW Human
Huynh N, VonMoss L, Smith D et al. Characterization of Regulatory Extracellular Vesicles from Osteoclasts. J Dent Res 2016 Jun [PMID: 26908631]
Watari K, Shibata T, Nabeshima H et al. Impaired differentiation of macrophage lineage cells attenuates bone remodeling and inflammatory angiogenesis in Ndrg1 deficient mice. Sci Rep. 2016 Jan 18 [PMID: 26778110] (WB, Mouse)

Details:
RANK antibody used for WB on BM-derived macrophages (BMDMs) from WT and KO mice at a dilution of 1:1000 (Figure 3h).
WB Mouse
Kaneko YS, Ota A, Nakashima A et al. Lipopolysaccharide treatment arrests the cell cycle of BV-2 microglial cells in G1 phase and protects them from UV light-induced apoptosis. J Neural Transm. 2014 Jun 12 [PMID: 24919883] (WB, Mouse)

Details:
Fig 3: BV-2 immortalized mouse microglial cell line. Procaspase and active/cleaved caspase were observed at 32 kDa and 17 kDa respectively.
WB Mouse
Das S, Sepahi I, Duthie A et al. RANk receptor oligomerisation in the regulation of NFkB signalling. J. Mol. Endocrinol. 2014 May 23 [PMID: 24859969] (WB, Mouse)

Details:
HeLa cells transfected with mouse WT-RANkmyc, W434X-RANkmyc or G280X-RANkmyc constructs, Fig 3Bi. The specifically of RANk mAb was validated by WB with recombinant expressed mouse WT-RANkmyc (80 kDa) and W434X-RANkmyc (55 kDa). The RANk mAb did not detect
WB Mouse
Izawa T, Zou W, Chappel JC et al. c-Src links a RANK/avB3 integrin complex to the osteoclast cytoskeleton. Mol Cell Biol. 2012 Jul [PMID: 22615494] (WB, Mouse)

Details:
RANk (IMG-128A). 1. WB: Mice Splenocytes, Figure 1, 2C, 2D, & 2E.
WB Mouse
Riegel A, Maurer T, Prior B et al. Human polymorphonuclear neutrophils express RANK and are activated by its ligand, RANKL. Eur J Immunol. 2012 Apr [PMID: 22531921]

Details:
Antibody cited: RANK-PE, clone 9A725 (IMG-128D) for Flow (cell surface) + Flow (intracellular): 1. Whole blood and isolated polymorhonuclear neutrophils (PMNs): Figs 1, 3, 4, S1-S3. 3BS1, S2, polymorphonuclear neutrophils (PMNs) Notes: 1. RANK expression
Huang G, Wen Q, Gao Q et al. An efficient and rapid method for cDNA cloning from difficult templates using codon optimization and SOE-PCR: with human RANK and TIMP2 gene as examples. Biotechnol Lett. 2011 Oct [PMID: 21660576] (WB)

Details:
Antibody cited: RANK, clone 9A725 (IMG-128A). 1. WB: Fig 4b (RANK-transfected A549 cells). Recombinantly expressed RANK was dected at 97 kDa. A549 transfected with an empty plasmid were used as a negative control as A549 cells lack endogenous expression o
WB
Crockett JC, Mellis DJ, Shennan KI et al. Signal peptide mutations in RANK prevent downstream activation of NF-kB. J Bone Miner Res. 2011 Aug [PMID: 21472776] (WB)

Details:
RANk/TNF11aR/ODFR (IMG-128A). WB: 293 cells transfected with RANk, Fig 1. Note: The specificityof the RANk antibody (IMG-128A) was transfected validated by WB in Rank transfected 293 cell line, Fig 1.
WB
Ayon Haro ER, Ukai T, Yokoyama M et al. Locally administered interferon-gamma accelerates lipopolysaccharide-induced osteoclastogenesis independent of immunohistological RANKL upregulation. J Periodontal Res. 2011 Jun [PMID: 21361961]

Details:
RANK-PE, clone 9A725 (IMG-128D). Flow (cell surface): Primary mouse bone marrow macrophages. By flow cytometry analysis, ~95% of the bone marrow macrophage population was positive for RANK and CD11b (data described on page 363). Note: The RANK-PE mAb was used to help characterize the primary bone macrophages isolated from mouse tibiae and femurs (see Material and Methods, page 363).
Show All 16 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for RANK/TNFRSF11A Antibody (NB100-56508). (Showing 1 - 3 of 3 FAQs).

  1. Will I be able to use your stain for histological detection of RANK in bone sections for rat?
    • This antibody has been validated in IHC in paraffin-embedded tissues and is suitable for use on mouse and human samples. The rat RANK is 91% similar to mouse RANK, so this antibody is predicted to cross-react.
  2. Would you be able to confirm that Raw 264.7 cells were used in the flow cytometry validation of NB100-56508 - RANK/TNFRSF11A Antibody (9A725)?
    • Yes the cells used were Raw 264.7 cells.
  3. My lab has purchased RANK (clone 9A725) for IHC optimization in breast TMA sections. Can you recommend appropriate positive and negative control tissues?
    • Human Protein Atlas: http://www.proteinatlas.org/ENSG00000141655-TNFRSF11A/tissue. I see that brain Cerebral cortex/Hippocampus/Lateral ventricle/Cerebellum all have very good expression. Moderate high expression will be Fallopian tube/Stomach/Duodenum/Small intestine. Moderate low expression that compares to breast will be Cervix/ uterine/Endometrium. Negative expression will be Ovary/Oral mucosa/Salivary gland/Esophagus. Please check the website for other choices.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56508AF405
Alexa Fluor 488 Labeled NB100-56508AF488
Alexa Fluor 647 Labeled NB100-56508AF647
Alexa Fluor 700 Labeled NB100-56508AF700
Allophycocyanin Labeled NB100-56508APC
Biotin Labeled NB100-56508B
DyLight 350 Labeled NB100-56508UV
DyLight 405 Labeled NB100-56508V
DyLight 488 Labeled NB100-56508G
DyLight 550 Labeled NB100-56508R
DyLight 650 Labeled NB100-56508C
DyLight 680 Labeled NB100-56508FR
DyLight 755 Labeled NB100-56508IR
HRP Labeled NB100-56508H
PE Labeled NB100-56029
PerCP Labeled NB100-56508PCP

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Bioinformatics

Gene Symbol TNFRSF11A
Entrez
Uniprot