IL-1 RI Antibody [Unconjugated]

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IL‑1 RI was detected in perfusion fixed frozen sections of mouse skin (tail) using Goat Anti-Mouse IL‑1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF771) at 1 µg/mL for 1 hour at ...read more
Recombinant Mouse IL‑1 alpha /IL‑1F1 (Catalog # 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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IL-1 RI Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-1 RI
Leu20-Lys338
Accession # P13504
Specificity
Detects mouse IL-1 RI in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse IL-18 R and recombinant human IL-1 RI is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL1R1
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1-15 ug/mL
  • Neutralization 0.2-1.0 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF771 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 RI Antibody [Unconjugated]

  • CD121 antigen-like family member A
  • CD121a antigen
  • CD121a
  • IL-1 RI
  • IL1R
  • IL1R1
  • IL1RI
  • IL-1RI
  • IL1RT1
  • IL-1RT1
  • IL-1RT-1
  • Interleukin 1 Receptor 1
  • interleukin 1 receptor, type I
  • interleukin receptor 1
  • interleukin-1 receptor type 1
  • p80

Background

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence identity in their extracellular domains. The two receptor types do not heterodimerize into a receptor complex. Mouse IL-1 RI shares 63% amino acid sequence homology with human IL-1 RI in their extracellular domains.

An IL-1 receptor accessory protein (1) that can heterodimerize with the Type I receptor in the presence of IL-1 alpha or IL-1 beta  but not IL-1ra, was identified. This Type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor Type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor Type I is a potent antagonist of IL-1 action.

  1. Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 RI Antibody (AF771)(24)

We have publications tested in 3 confirmed species: Mouse, Rat, Primate - Macaca fuscata (Japanese Macaque).

We have publications tested in 8 applications: ELISA Development, ICC, IHC, IHC-Fr, Immunoprecipitation, In Vivo, Neutralization, Western Blot.


Filter By Application
ELISA Development
(1)
ICC
(1)
IHC
(7)
IHC-Fr
(3)
Immunoprecipitation
(1)
In Vivo
(1)
Neutralization
(2)
Western Blot
(4)
All Applications
Filter By Species
Mouse
(15)
Rat
(2)
Primate - Macaca fuscata (Japanese Macaque)
(1)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using AF771 Applications Species
Evan Kidder, Meleah Pea, Siyuan Cheng, Satya-Priya Koppada, Suren Visvanathan, Quartina Henderson et al. The interleukin-1 receptor type-1 in disturbed flow-induced endothelial mesenchymal activation Frontiers in Cardiovascular Medicine 2023-07-19 [PMID: 37539090]
Yamamoto, T;Kase, S;Shinkai, A;Murata, M;Kikuchi, K;Wu, D;Kageyama, Y;Shinohara, M;Sasase, T;Ishida, S; Phosphorylation of alpha B-Crystallin Involves Interleukin-1 beta -Mediated Intracellular Retention in Retinal Müller Cells: A New Mechanism Underlying Fibrovascular Membrane Formation Investigative ophthalmology & visual science 2023-07-03 [PMID: 37459063] (Neutralization, Rat) Neutralization Rat
Edamakanti, CR;Mohan, V;Opal, P; Reactive Bergmann glia play a central role in spinocerebellar ataxia inflammation via the JNK pathway Journal of neuroinflammation 2023-05-26 [PMID: 37237366] (IHC, Mouse) IHC Mouse
FP Varodayan, AR Pahng, TD Davis, P Gandhi, M Bajo, MQ Steinman, WB Kiosses, YA Blednov, MD Burkart, S Edwards, AJ Roberts, M Roberto Chronic ethanol induces a pro-inflammatory switch in interleukin-1beta regulation of GABAergic signaling in the medial prefrontal cortex of male mice Brain, Behavior, and Immunity, 2023;110(0):125-139. 2023 [PMID: 36863493] (Western Blot, Mouse) Western Blot Mouse
FP Varodayan, AR Pahng, TD Davis, P Gandhi, M Bajo, MQ Steinman, WB Kiosses, YA Blednov, MD Burkart, S Edwards, AJ Roberts, M Roberto Chronic ethanol induces a pro-inflammatory switch in interleukin-1beta regulation of GABAergic signaling in the medial prefrontal cortex of male mice Brain, Behavior, and Immunity, 2023-02-28;110(0):125-139. 2023-02-28 [PMID: 36863493] (Western Blot, Mouse) Western Blot Mouse
A Villatoro, V Cuminetti, A Bernal, C Torroja, I Cossío, A Benguría, M Ferré, J Konieczny, E Vázquez, A Rubio, P Utnes, X You, CG Fenton, RH Paulssen, J Zhang, F Sánchez-Ca, A Dopazo, A Vik, E Anderssen, A Hidalgo, L Arranz Endogenous IL-1 receptor antagonist restricts healthy and malignant myeloproliferation Nature Communications, 2023-01-03;14(1):12. 2023-01-03 [PMID: 36596811] (IHC, Mouse) IHC Mouse
MF Rahman, Y Yang, BT Le, A Dutta, J Posyniak, P Faughnan, MA Sayem, NS Aguilera, G Mohi Interleukin-1 contributes to clonal expansion and progression of bone marrow fibrosis in JAK2V617F-induced myeloproliferative neoplasm Nature Communications, 2022-09-13;13(1):5347. 2022-09-13 [PMID: 36100596] (In Vivo, Mouse) In Vivo Mouse
R Furrer, AJ Jauch, T Nageswara, S Dilbaz, P Rhein, SA Steurer, M Recher, RC Skoda, C Handschin Remodeling of metabolism and inflammation by exercise ameliorates tumor-associated anemia Science Advances, 2021-09-08;7(37):eabi4852. 2021-09-08 [PMID: 34516881] (Immunoprecipitation, Western Blot, Mouse) Immunoprecipitation, Western Blot Mouse
Neil Dani, Rebecca H. Herbst, Cristin McCabe, Gilad S. Green, Karol Kaiser, Joshua P. Head et al. A cellular and spatial map of the choroid plexus across brain ventricles and ages Cell 2021-05-01 [PMID: 33932339]
A Shimada, S Hasegawa-I Increased cytokine expression in the choroid plexus stroma and epithelium in response to endotoxin-induced systemic inflammation in mice Toxicology reports, 2021-03-10;8(0):520-528. 2021-03-10 [PMID: 33747797] (IHC, Mouse) IHC Mouse
Show All 24 Publications.

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Bioinformatics

Gene Symbol IL1R1
Uniprot