IL-1 RI Antibody

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Recombinant Mouse IL‑1 alpha /IL‑1F1 (Catalog # 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, Neut
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price

IL-1 RI Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-1 RI
Leu20-Lys338
Accession # P13504
Specificity
Detects mouse IL-1 RI in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) IL-18 R is observed and 5% cross-reactivity with rmIL-18 R beta, recombinant human IL-1 RI, and recombinant rat IL-1 Rrp2 is observed and less than 1% cross-reactivity with rmIL-1 RII is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL1R1
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.2-1.0 ug/mL
Publications
Read Publications using
AF771 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 RI Antibody

  • CD121 antigen-like family member A
  • CD121a antigen
  • CD121a
  • IL-1 RI
  • IL1R
  • IL1R1
  • IL1RI
  • IL-1RI
  • IL1RT1
  • IL-1RT1
  • IL-1RT-1
  • Interleukin 1 Receptor 1
  • interleukin 1 receptor, type I
  • interleukin receptor 1
  • interleukin-1 receptor type 1
  • p80

Background

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence identity in their extracellular domains. The two receptor types do not heterodimerize into a receptor complex. Mouse IL-1 RI shares 63% amino acid sequence homology with human IL-1 RI in their extracellular domains.

An IL-1 receptor accessory protein (1) that can heterodimerize with the Type I receptor in the presence of IL-1 alpha or IL-1 beta  but not IL-1ra, was identified. This Type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor Type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor Type I is a potent antagonist of IL-1 action.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-1 RI Antibody (AF771)(8)

We have publications tested in 3 confirmed species: Mouse, Rat, Primate - Macaca fuscata (Japanese Macaque).

We have publications tested in 6 applications: ELISA Development, ICC, IHC, IHC - Frozen, IHC Frozen, WB.


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ELISA Development
(1)
ICC
(1)
IHC
(3)
IHC - Frozen
(1)
IHC Frozen
(1)
WB
(1)
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Filter By Species
Mouse
(6)
Rat
(1)
Primate - Macaca fuscata (Japanese Macaque)
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Showing Publications 1 - 8 of 8.
Publications using AF771 Applications Species
Spinal astrocytic activation contributes to mechanical allodynia in a rat model of cyclophosphamide-induced cystitis Mol Pain, 2016;12(0):. 2016 [PMID: 27852964] (IHC - Frozen,, Rat) IHC - Frozen Rat
Aube B, Levesque S, Pare A, Chamma E, Kebir H, Gorina R, Lecuyer M, Alvarez J, De Koninck Y, Engelhardt B, Prat A, Cote D, Lacroix S Neutrophils mediate blood-spinal cord barrier disruption in demyelinating neuroinflammatory diseases. J Immunol, 2014;193(5):2438-54. 2014 [PMID: 25049355] (IHC,, Mouse) IHC Mouse
Ataka K, Asakawa A, Nagaishi K, Kaimoto K, Sawada A, Hayakawa Y, Tatezawa R, Inui A, Fujimiya M Bone marrow-derived microglia infiltrate into the paraventricular nucleus of chronic psychological stress-loaded mice. PLoS ONE, 2013;8(11):e81744. 2013 [PMID: 24303068] (IHC,, Mouse) IHC Mouse
Proebstl D, Voisin MB, Woodfin A, Whiteford J, D'Acquisto F, Jones GE, Rowe D, Nourshargh S Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo. J. Exp. Med., 2012;209(6):1219-34. 2012 [PMID: 22615129] (IHC,, Mouse) IHC Mouse
Imamura Y, Wang H, Matsumoto N, Muroya T, Shimazaki J, Ogura H, Shimazu T Interleukin-1beta causes long-term potentiation deficiency in a mouse model of septic encephalopathy. Neuroscience, 2011;187(0):63. 2011 [PMID: 21571042] (WB,, Mouse) WB Mouse
Grayson BE, Levasseur PR, Williams SM, Smith MS, Marks DL, Grove KL Changes in melanocortin expression and inflammatory pathways in fetal offspring of nonhuman primates fed a high-fat diet. Endocrinology, 2010;151(4):1622-32. 2010 [PMID: 20176722] (IHC Frozen,, Primate - Macaca fuscata (Japanese Macaque)) IHC Frozen Primate - Macaca fuscata (Japanese Macaque)
O&amp;amp;apos;Connor JC, Sherry CL, Guest CB, Freund GG Type 2 diabetes impairs insulin receptor substrate-2-mediated phosphatidylinositol 3-kinase activity in primary macrophages to induce a state of cytokine resistance to IL-4 in association with overexpression of suppressor of cytokine signaling-3. J. Immunol., 2007;178(11):6886-93. 2007 [PMID: 17513737] (ELISA Development,, Mouse) ELISA Development Mouse
Davis CN, Tabarean I, Gaidarova S, Behrens MM, Bartfai T IL-1beta induces a MyD88-dependent and ceramide-mediated activation of Src in anterior hypothalamic neurons. J. Neurochem., 2006;98(5):1379-89. 2006 [PMID: 16771830] (ICC,, Mouse) ICC Mouse

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Bioinformatics

Gene Symbol IL1R1
Entrez
Uniprot