IL-1 RI Antibody [Unconjugated]

IL‑1 RI was detected in perfusion fixed frozen sections of mouse skin (tail) using Goat Anti-Mouse IL‑1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF771) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing central nervous system. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Recombinant Mouse IL‑1 alpha /IL‑1F1 (Catalog # 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑1 alpha /IL‑1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF771). The ND50 is typically 0.2-1.0 µg/mL.

• Reactivity: Mu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-1 RI Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL-1 RI
  Leu20-Lys338
  Accession # P13504
• Specificity: Detects mouse IL-1 RI in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse IL-18 R and recombinant human IL-1 RI is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL1R1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-15 ug/mL
  • Neutralization 0.2-1.0 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 RI Antibody [Unconjugated]

• CD121 antigen-like family member A
• CD121a antigen
• CD121a
• IL-1 RI
• IL1R
• IL1R1
• IL1RI
• IL-1RI
• IL1RT1
• IL-1RT1
• IL-1RT-1
• Interleukin 1 Receptor 1
• interleukin 1 receptor, type I
• interleukin receptor 1
• interleukin-1 receptor type 1
• p80

Background

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence identity in their extracellular domains. The two receptor types do not heterodimerize into a receptor complex. Mouse IL-1 RI shares 63% amino acid sequence homology with human IL-1 RI in their extracellular domains.

An IL-1 receptor accessory protein (1) that can heterodimerize with the Type I receptor in the presence of IL-1 alpha or IL-1 beta  but not IL-1ra, was identified. This Type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor Type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor Type I is a potent antagonist of IL-1 action.

1. Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 RI Antibody (AF771)(12)

Publications using AF771

• R Furrer, AJ Jauch, T Nageswara, S Dilbaz, P Rhein, SA Steurer, M Recher, RC Skoda, C Handschin Remodeling of metabolism and inflammation by exercise ameliorates tumor-associated anemia Science Advances, 2021;7(37):eabi4852. 2021 [PMID: 34516881] (Immunoprecipitation, Mouse)
• A Shimada, S Hasegawa-I Increased cytokine expression in the choroid plexus stroma and epithelium in response to endotoxin-induced systemic inflammation in mice Toxicology reports, 2021;8(0):520-528. 2021 [PMID: 33747797] (IHC, Mouse)
• HM Hsiao, R Fernandez, S Tanaka, W Li, JH Spahn, S Chiu, M Akbarpour, D Ruiz-Perez, Q Wu, C Turam, D Scozzi, T Takahashi, HP Luehmann, V Puri, GRS Budinger, AS Krupnick, AV Misharin, KJ Lavine, Y Liu, AE Gelman, A Bharat, D Kreisel Spleen-derived classical monocytes mediate lung ischemia-reperfusion injury through IL-1? J. Clin. Invest., 2018;128(7):2833-2847. 2018 [PMID: 29781811] (Neutralization, Mouse)
• DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2018;15(1):39-46. 2018 [PMID: 29320487] (IHC, Mouse)
• Spinal astrocytic activation contributes to mechanical allodynia in a rat model of cyclophosphamide-induced cystitis Mol Pain, 2016;12(0):. 2016 [PMID: 27852964] (IHC-Fr, Rat)
• Aube B, Levesque S, Pare A, Chamma E, Kebir H, Gorina R, Lecuyer M, Alvarez J, De Koninck Y, Engelhardt B, Prat A, Cote D, Lacroix S Neutrophils mediate blood-spinal cord barrier disruption in demyelinating neuroinflammatory diseases. J Immunol, 2014;193(5):2438-54. 2014 [PMID: 25049355] (IHC, Mouse)
• Ataka K, Asakawa A, Nagaishi K, Kaimoto K, Sawada A, Hayakawa Y, Tatezawa R, Inui A, Fujimiya M Bone marrow-derived microglia infiltrate into the paraventricular nucleus of chronic psychological stress-loaded mice. PLoS ONE, 2013;8(11):e81744. 2013 [PMID: 24303068] (IHC, Mouse)
• Proebstl D, Voisin MB, Woodfin A, Whiteford J, D'Acquisto F, Jones GE, Rowe D, Nourshargh S Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo. J. Exp. Med., 2012;209(6):1219-34. 2012 [PMID: 22615129] (IHC, Mouse)
• Imamura Y, Wang H, Matsumoto N, Muroya T, Shimazaki J, Ogura H, Shimazu T Interleukin-1beta causes long-term potentiation deficiency in a mouse model of septic encephalopathy. Neuroscience, 2011;187(0):63. 2011 [PMID: 21571042] (Western Blot, Mouse)
• Grayson BE, Levasseur PR, Williams SM, Smith MS, Marks DL, Grove KL Changes in melanocortin expression and inflammatory pathways in fetal offspring of nonhuman primates fed a high-fat diet. Endocrinology, 2010;151(4):1622-32. 2010 [PMID: 20176722] (IHC-Fr, Primate - Macaca fuscata (Japanese Macaque))
• O&amp;amp;apos;Connor JC, Sherry CL, Guest CB, Freund GG Type 2 diabetes impairs insulin receptor substrate-2-mediated phosphatidylinositol 3-kinase activity in primary macrophages to induce a state of cytokine resistance to IL-4 in association with overexpression of suppressor of cytokine signaling-3. J. Immunol., 2007;178(11):6886-93. 2007 [PMID: 17513737] (ELISA Development, Mouse)
• Davis CN, Tabarean I, Gaidarova S, Behrens MM, Bartfai T IL-1beta induces a MyD88-dependent and ceramide-mediated activation of Src in anterior hypothalamic neurons. J. Neurochem., 2006;98(5):1379-89. 2006 [PMID: 16771830] (ICC, Mouse)

Bioinformatics

• Gene Symbol: IL1R1
• Uniprot:
  • P13504()