p21/CIP1/CDKN1A Antibody (WA-1)


Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).

Product Details

Reactivity Hu, Mu, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
0.2 mg/ml

Order Details

p21/CIP1/CDKN1A Antibody (WA-1) Summary

Human recombinant p21/CIP1/CDKN1A protein (Uniprot: P38936 )
This monoclonal antibody recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This monoclonal antibody is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdks and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma). In ELISA, monoclonal antibody WA-1 is useful either as a solid phase or for detection of p21 protein.
IgG1 Kappa
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Use in Western blot reported in scientific literature (PMID 28137862).
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NBP2-29463 in the following applications:

  • IHC
    2 publications
  • 2 publications
  • WB
    8 publications

Packaging, Storage & Formulations

Store at 4C.
10 mM PBS with 0.05% BSA
0.05% Sodium Azide
0.2 mg/ml
Protein A or G purified


200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33084)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for p21/CIP1/CDKN1A Antibody (WA-1)

  • CAP20cyclin-dependent kinase inhibitor 1
  • CDK-interacting protein 1
  • CDKN1A
  • CDKN1melanoma differentiation associated protein 6
  • CIP1
  • CIP1WAF1CDK-interaction protein 1
  • cyclin-dependent kinase inhibitor 1A (p21, Cip1)
  • MDA6
  • MDA-6
  • Melanoma differentiation-associated protein 6
  • p21
  • p21CIP1
  • p21Cip1/Waf1
  • PIC1
  • SDI1DNA synthesis inhibitor
  • wild-type p53-activated fragment 1


p21 (WAF1), a member of the cycle dependent kinase (CDK) inhibitor family, was first identified as a tumor suppressor or anti-oncogenic protein and negative regulator of the cell cycle. Over the years it became apparent that p21 has broad functions in regulating fundamental cellular programs including proliferation, differentiation, migration, senescence, and has both anti-oncogenic and oncogenic properties (reviewed in Romanov, 2012). Many antibody and other studies have demonstrated that p21 levels are frequently elevated in situations associated with reduced proliferation, including differentiation and senescence, and decreased in proliferative states. Subcellular localization may significant and nuclear antibody staining may be indicative of p21 functioning as a cell cycle inhibitor, tumor suppressor, or in a senescence program. In contrast, cytoplasmic antibody staining may indicate p21 is acting an oncogene through regulatation of migration, proliferation, or apoptosis.

It is interesting to note that p21 expression is induced by wild type, but not mutant p53. The HJ21/WA-1 antibody clone has been used to demonstrate this phenomenon by western blot (Blaydes, 2001). The inability of mutant p53 to induce p21 essentially means that normal functions of p21 are compromised when p53 gene mutations are present. Since p53 gene mutations are present in up to 50% of human cancers, the loss of p21 function in cancer is significant.

In its normal functioning state, p21 binds to cyclin/CDK complexes. When it binds to these complexes, it inhibits their kinase activity which, in turn, stops cell cycle progression and hence p21 gains its reputation as a mitotic cell cycle inhibitor. In this regard, the antibody showed that decreased Cdk2-cyclin E1 activity corresponded with a decrease in cyclin E1 and increase in p21 protein levels (White, 2005).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p21/CIP1/CDKN1A Antibody (NBP2-29463)(16)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: IHC, IHC-P, WB.

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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NBP2-29463 Applications Species
Kaur N, Lum M, Lewis RE et al. A novel anti-proliferative PKCalpha-Ras-ERK signaling axis in intestinal epithelial cells The Journal of biological chemistry Jun 10 2022 [PMID: 35697074] (WB) WB
Saleh T, Alhesa A, Al-Balas M et al. Expression of therapy-induced senescence markers in breast cancer samples upon incomplete response to neoadjuvant chemotherapy Bioscience reports May 28 2021 [PMID: 33948615] (IHC, Human) IHC Human
Kaczorowski A, Tolstov Y, Falkenstein M et al. Rearranged ERG confers robustness to prostate cancer cells by subverting the function of p53 Urol. Oncol. Jul 13 2020 [PMID: 32674955] (WB, Human) WB Human
Couture R, Mora N, Al Bittar S, et al. Luteolin modulates gene expression related to steroidogenesis, apoptosis, and stress response in rat LC540 tumor Leydig cells Cell Biol. Toxicol. 2019 Jun 14 [PMID: 31201582]
Aratani S, Tagawa M, Nagasaka S et al. Radiation-induced premature cellular senescence involved in glomerular diseases in rats. Sci Rep. Nov 14 2018 [PMID: 30429495] (IHC-P, Rat) IHC-P Rat
Huang X, Huang R, Gou S et al. Platinum(IV) complexes conjugated with phenstatin analogue as inhibitors of microtubule polymerization and reverser of multidrug resistance Bioorg. Med. Chem. 2017 Jul 08 [PMID: 28728896] (Human) Human
Xiang J, Yang S, Xin N et al. DYRK1A regulates Hap1-Dcaf7/WDR68 binding with implication for delayed growth in Down syndrome Proc. Natl. Acad. Sci. U.S.A Jan 30 2017 [PMID: 28137862] (WB, Mouse) WB Mouse
Ge X, Pettan-Brewer C, Morton J et al. Mitochondrial catalase suppresses naturally occurring lung cancer in old mice. Pathobiol Aging Age Relat Dis 2015 Sep 24 [PMID: 26400209] (IHC, Mouse) IHC Mouse
Romanov VS, Pospelov VA, Pospelova TV. Cyclin-dependent kinase inhibitor p21(Waf1): contemporary view on its role in senescence and oncogenesis. Biochemistry (Mosc). 2012 Jun [PMID: 22817456]
Koike M, Yutoku Y, Koike A. Accumulation of p21 proteins at DNA damage sites independent of p53 and core NHEJ factors following irradiation. Biochem Biophys Res Commun. 2011 Aug 19 [PMID: 21787748]
Show All 16 Publications.

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  1. I would like to know the concentration of NBP2-29463.
    • NBP2-29463 (p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) has the concentration of 0.2 mg/ml.

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Gene Symbol CDKN1A