eNOS Antibody

Images

 
Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Expression of endothelial markers indicate functional endothelium in TEVG. Whole mount staining of native aorta and TEVG. VE-cadherin is a marker of cellular ...read more
Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Staining of eNOS in human lung tissue.
Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Mouse Carotid Artery stained with eNOS antibody. Image from verified customer review.
Immunohistochemistry: eNOS Antibody [NB300-500] - Staining of representative pulmonary artery endothelial cells from rats subjected to CON=Control Group, ALI=Acute lung injury Group, LV=Low tidal volume Group, VLV=Very ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Am, Bv, Ca, Dr, ShSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

eNOS Antibody Summary

Immunogen
Synthetic peptide corresponding to residues S(1179) LQERQLRGAVPWAFD(1194) of human eNOS.
Epitope
SLQERQLRGAVPWAFD
Specificity
This does not detect human inducible NOS or rat brain NOS.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOS3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 1:50-1:1000
Application Notes
In IHC, antigen retrieval is not essential but may optimize staining. By Western blot, a 140kDa band is seen in human endothelial cells. Optimal dilutions should be determined by the end user.
Theoretical MW
133 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB300-500 in the following applications:

Publications
Read Publications using
NB300-500 in the following applications:

  • IHC
    4 publications
  • WB
    7 publications

Reactivity Notes

Sheep reactivity reported in scientific literature (PMID: 31352175).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for eNOS Antibody

  • Constitutive NOS
  • EC 1.14.13.39
  • ECNOS
  • Endothelial NOS
  • endothelial
  • eNOS
  • eNOScNOS
  • nitric oxide synthase 3 (endothelial cell)
  • NOS type III
  • NOS3
  • NOSIII

Background

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-605
Species: Hu, Mu, Po, Rt
Applications: Flow, IB, ICC/IF, IHC, IHC-P, In vitro, WB
NB100-858
Species: Gp, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC-WhMt, IHC, IHC-Fr, IHC-P, PEP-ELISA, WB
NBP2-47415
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-76917
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
DVE00
Species: Hu
Applications: ELISA
NBP2-27084
Species: Hu, Pm
Applications: IHC, IHC-P, WB
QET00B
Species: Hu
Applications: ELISA
NBP1-30027
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
NB100-615
Species: Hu, Mu, Rt, Sh
Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
AF1513
Species: Mu
Applications: CyTOF-ready, Flow, IHC, IP, Simple Western, WB
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-67360
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP1-89993
Species: Hu
Applications: IHC, IHC-P, WB

Publications for eNOS Antibody (NB300-500)(19)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Canine, Porcine, Sheep.

We have publications tested in 2 applications: IHC, WB.


Filter By Application
IHC
(4)
WB
(7)
All Applications
Filter By Species
Human
(1)
Mouse
(4)
Rat
(3)
Canine
(1)
Porcine
(1)
Sheep
(1)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NB300-500 Applications Species
Patel PD, Chen YL, Kasetti RB et al. Impaired TRPV4-eNOS signaling in trabecular meshwork elevates intraocular pressure in glaucoma Proceedings of the National Academy of Sciences of the United States of America Apr 20 2021 [PMID: 33853948]
Ricardo-da-Silva FY, Armstrong R, Vidal-Dos-Santos M Et al. 17 beta-Estradiol Treatment Protects Lungs Against Brain Death Effects in Female Rat Donor Transplantation Apr 1 2021 [PMID: 33031230]
Gao XX, Zhang QF, Zhu M et al. Effects of l-arginine on endometrial estrogen receptor alpha/beta and progesterone receptor expression in nutrient-restricted sheep Theriogenology Jul 25 2019 [PMID: 31352175] (WB, Sheep) WB Sheep
Fujita M, Tanaka N, Sakaguchi Y et al. Small caliber heparin loaded ultrafine microfiber woven graft achieved high patency rate in a preliminary study of canine carotid artery implantation JVS: Vascular Science Jan 1 2020 (IHC, Canine) IHC Canine
Ottolini M, Daneva Z, Chen YL et al. Mechanisms underlying selective coupling of endothelial Ca2+ signals with eNOS versus IK/SK channels in systemic and pulmonary arteries J. Physiol. (Lond.) May 28 2020 [PMID: 32463112] (WB) WB
Patel G, Fury W, Yang H et al. Molecular taxonomy of human ocular outflow tissues defined by single cell transcriptomics bioRxiv Jan 1 2020 (Human) Human
CeYlan B, OzansoY M, kilic u et al. N-acetylcysteine suppresses colistimethate sodium-induced nephrotoxicity via activation of SOD2, eNOS, and MMP3 protein expressions. Ren Fail. Nov 1 2018 [PMID: 30035652] (WB, Rat) WB Rat
Zhang L, Zheng H, Luo J et al. Inhibition of endothelial nitric oxide synthase reverses the effect of exercise on improving cognitive function in hypertensive rats. Hypertens. Res. Mar 22 2018 [PMID: 29568075] (IHC, Rat) IHC Rat
Leao VF, Ferreira LLDM, Melo CM, Bonfleur ML. Taurine supplementation prevents endothelial dysfunction and attenuates structural changes in aortas from hypothalamic obese rats. Eur J Nutr. 2018 Jan 27 [PMID: 29374793] (WB, Rat) WB Rat
Nelson RK, Ya-Ping J, Gadbery J et al. Phospholipase D2 loss results in increased blood pressure via inhibition of the endothelial nitric oxide synthase pathway Sci Rep 2017 Aug 22 [PMID: 28831159] (WB, Mouse) WB Mouse
Show All 19 Publications.

Reviews for eNOS Antibody (NB300-500) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using NB300-500:
Filter by Applications
WB
(1)
IHC
(1)
IHC-P
(1)
All Applications
Filter by Species
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
WB Mouse 05/23/2017
View

Summary

ApplicationWestern Blot
Sample Tested Mouse brain
SpeciesMouse
  5
reviewed by:
Verified Customer
IHC Mouse 05/23/2017
View

Summary

ApplicationImmunohistochemistry
Sample Tested Mouse brain
SpeciesMouse
Immunohistochemistry-Paraffin eNOS NB300-500
Enlarge
5
reviewed by:
Verified Customer
IHC-P Mouse 02/22/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Carotid Artery
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for eNOS Antibody (NB300-500) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB300-500AF350
Alexa Fluor 405 NB300-500AF405
Alexa Fluor 488 NB300-500AF488
Alexa Fluor 532 NB300-500AF532
Alexa Fluor 594 NB300-500AF594
Alexa Fluor 647 NB300-500AF647
Alexa Fluor 700 NB300-500AF700
Alexa Fluor 750 NB300-500AF750
Biotin NB300-500B
DyLight 350 NB300-500UV
DyLight 405 NB300-500V
DyLight 488 NB300-500G
DyLight 550 NB300-500R
DyLight 594 NB300-500DL594
DyLight 650 NB300-500C
DyLight 680 NB300-500FR
DyLight 755 NB300-500IR
FITC NB300-500F
HRP NB300-500H
Janelia Fluor 549 NB300-500JF549
Janelia Fluor 646 NB300-500JF646

Additional eNOS Products

Bioinformatics Tool for eNOS Antibody (NB300-500)

Discover related pathways, diseases and genes to eNOS Antibody (NB300-500). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for eNOS Antibody (NB300-500)

Discover more about diseases related to eNOS Antibody (NB300-500).
 

Pathways for eNOS Antibody (NB300-500)

View related products by pathway.

PTMs for eNOS Antibody (NB300-500)

Learn more about PTMs related to eNOS Antibody (NB300-500).
 

Research Areas for eNOS Antibody (NB300-500)

Find related products by research area.

Blogs on eNOS

There are no specific blogs for eNOS, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

 
Verified Customer
05/23/2017
Application: WB
Species: Mouse

 
Verified Customer
05/23/2017
Application: IHC
Species: Mouse

Verified Customer
02/22/2016
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol NOS3
Entrez
Uniprot