eNOS Antibody

Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Expression of endothelial markers indicate functional endothelium in TEVG. Whole mount staining of native aorta and TEVG. VE-cadherin is a marker of cellular borders of endothelial cells (green). Endothelial nitric oxide synthase (eNOS) is a marker of a functional endothelium (red). DAPI is a nuclear stain (blue). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120328), licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Staining of eNOS in human lung tissue.

Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Mouse Carotid Artery stained with eNOS antibody. Image from verified customer review.

Immunohistochemistry: eNOS Antibody [NB300-500] - Staining of representative pulmonary artery endothelial cells from rats subjected to CON=Control Group, ALI=Acute lung injury Group, LV=Low tidal volume Group, VLV=Very low tidal volume Group, MV=Large tidal volume Group. Dark brown staining of indicates eNOS-positive cells. Magnification x400.

• Reactivity: Hu, Mu, Rt, Po, Am, Bv, Ca, Dr, Sh
• Applications: WB, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

eNOS Antibody Summary

• Immunogen: Synthetic peptide corresponding to residues S(1179) LQERQLRGAVPWAFD(1194) of human eNOS.
• Epitope: SLQERQLRGAVPWAFD
• Specificity: This does not detect human inducible NOS or rat brain NOS.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NOS3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 1:50-1:1000
• Application Notes: In IHC, antigen retrieval is not essential but may optimize staining. By Western blot, a 140kDa band is seen in human endothelial cells. Optimal dilutions should be determined by the end user.
• Theoretical MW: 133 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Sheep reactivity reported in scientific literature (PMID: 31352175).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for eNOS Antibody

• Constitutive NOS
• EC 1.14.13.39
• ECNOS
• Endothelial NOS
• endothelial
• eNOS
• eNOScNOS
• nitric oxide synthase 3 (endothelial cell)
• NOS type III
• NOS3
• NOSIII

Background

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for eNOS Antibody (NB300-500)(19)

Publications using NB300-500

• Patel PD, Chen YL, Kasetti RB et al. Impaired TRPV4-eNOS signaling in trabecular meshwork elevates intraocular pressure in glaucoma Proceedings of the National Academy of Sciences of the United States of America Apr 20 2021 [PMID: 33853948]
• Ricardo-da-Silva FY, Armstrong R, Vidal-Dos-Santos M Et al. 17 beta-Estradiol Treatment Protects Lungs Against Brain Death Effects in Female Rat Donor Transplantation Apr 1 2021 [PMID: 33031230]
• Gao XX, Zhang QF, Zhu M et al. Effects of l-arginine on endometrial estrogen receptor alpha/beta and progesterone receptor expression in nutrient-restricted sheep Theriogenology Jul 25 2019 [PMID: 31352175] (WB, Sheep)
• Fujita M, Tanaka N, Sakaguchi Y et al. Small caliber heparin loaded ultrafine microfiber woven graft achieved high patency rate in a preliminary study of canine carotid artery implantation JVS: Vascular Science Jan 1 2020 (IHC, Canine)
• Ottolini M, Daneva Z, Chen YL et al. Mechanisms underlying selective coupling of endothelial Ca2+ signals with eNOS versus IK/SK channels in systemic and pulmonary arteries J. Physiol. (Lond.) May 28 2020 [PMID: 32463112] (WB)
• Patel G, Fury W, Yang H et al. Molecular taxonomy of human ocular outflow tissues defined by single cell transcriptomics bioRxiv Jan 1 2020 (Human)
• CeYlan B, OzansoY M, kilic u et al. N-acetylcysteine suppresses colistimethate sodium-induced nephrotoxicity via activation of SOD2, eNOS, and MMP3 protein expressions. Ren Fail. Nov 1 2018 [PMID: 30035652] (WB, Rat)
• Zhang L, Zheng H, Luo J et al. Inhibition of endothelial nitric oxide synthase reverses the effect of exercise on improving cognitive function in hypertensive rats. Hypertens. Res. Mar 22 2018 [PMID: 29568075] (IHC, Rat)
• Leao VF, Ferreira LLDM, Melo CM, Bonfleur ML. Taurine supplementation prevents endothelial dysfunction and attenuates structural changes in aortas from hypothalamic obese rats. Eur J Nutr. 2018 Jan 27 [PMID: 29374793] (WB, Rat)
• Nelson RK, Ya-Ping J, Gadbery J et al. Phospholipase D2 loss results in increased blood pressure via inhibition of the endothelial nitric oxide synthase pathway Sci Rep 2017 Aug 22 [PMID: 28831159] (WB, Mouse)
• Oczkowicz G, Caban A, Budzinski G et al. Endothelial Nitric Oxide Synthase and Endothelin-1 Expression in the Early Post-Porcine Kidney Autotransplantation Period. Transplant. Proc. Jun 1 2016 [PMID: 27496502] (WB, Porcine)
• Xia N, Horke S et al. Uncoupling of Endothelial Nitric Oxide Synthase in Perivascular Adipose Tissue of Diet-Induced Obese Mice. Arterioscler Thromb Vasc Biol Jan 1 2016 [PMID: 26586660] (IHC, Mouse)
• Andrukhova Olena, Slavic Svetlana, Odorfer Kathrin I et al. Experimental Myocardial Infarction Upregulates Circulating Fibroblast Growth Factor-23. Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research 2015 [PMID: 25858796] (WB, Mouse)
• Kurobe H, Maxfield MW, Tara S et al. Development of small diameter nanofiber tissue engineered arterial grafts PLoS ONE 2015 Apr 02 [PMID: 25830942] (IHC, Mouse)
• Oh YK, Bachar AR, Zacharias DG et al. Humanin Preserves Endothelial Function and Prevents Atherosclerotic Plaque Progression in Hypercholesterolemic ApoE Deficient Mice. Atherosclerosis;219(1):65-73. 2011 Nov. [PMID: 21763658]
• HOGBEN L. Risk of jaundice following transfusion with pooled plasma or serum. Br J Soc Med 1947 Jul [PMID: 20253608]
• Shirasuna K, Asahi T, Sasaki M et al. Distribution of arteriolovenous vessels, capillaries and eNOS expression in the bovine corpus luteum during the estrous cycle: a possible implication of different sensitivity by luteal phase to PGF(2alpha) in the increase of luteal blood flow. J Reprod Dev. 2010 Feb. [PMID: 19893278]
• Wang M, Lung MA. Acetylcholine induces contractile and relaxant effects in canine nasal venous systems. Eur Respir J. 2006 Oct. [PMID: 16737988]
• Guo FH, Comhair SA, Zheng S et al. Molecular mechanisms of increased nitric oxide (NO) in asthma: evidence for transcriptional and post-translational regulation of NO synthesis. J Immunol. 2000 Jun. [PMID: 10820280]

Reviews for eNOS Antibody (NB300-500) (3)

Average Rating: 4.7

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 05/23/2017

Summary

• Application: Western Blot
• Sample Tested: Mouse brain
• Species: Mouse

reviewed by: Verified Customer IHC Mouse 05/23/2017

Summary

• Application: Immunohistochemistry
• Sample Tested: Mouse brain
• Species: Mouse

reviewed by: Verified Customer IHC-P Mouse 02/22/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse Carotid Artery
• Species: Mouse

Bioinformatics

• Gene Symbol: NOS3
• Entrez:
  • 4846(Human)
  • 18127(Mouse)
  • 24600(Rat)
• Uniprot:
  • P29474()