eNOS Antibody - BSA Free

Images

 
Biological Strategies: Western Blot: eNOS Antibody - BSA Free [NB300-500] - Fractionated, compared to single exposure, radiation more profoundly suppressed TM and eNOS. Representative Western blot analysis and ...read more
Immunohistochemistry-Paraffin: eNOS Antibody - BSA Free [NB300-500] - Expression of endothelial markers indicate functional endothelium in TEVG. Whole mount staining of native aorta and TEVG. VE-cadherin is a marker of ...read more
Immunohistochemistry-Paraffin: eNOS Antibody - BSA Free [NB300-500] - Staining of eNOS in human lung tissue.
Immunohistochemistry-Paraffin: eNOS Antibody - BSA Free [NB300-500] - Mouse Carotid Artery stained with eNOS antibody. Image from verified customer review.
Immunohistochemistry: eNOS Antibody - BSA Free [NB300-500] - Staining of representative pulmonary artery endothelial cells from rats subjected to CON=Control Group, ALI=Acute lung injury Group, LV=Low tidal volume ...read more
Expression of endothelial markers indicate functional endothelium in TEVG.A. Whole mount staining of native aorta and TEVG. VE-cadherin is a marker of cellular borders of endothelial cells (green). Endothelial nitric ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Am, Bv, Ca, Dr, ShSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

eNOS Antibody - BSA Free Summary

Immunogen
Synthetic peptide corresponding to residues S(1179) LQERQLRGAVPWAFD(1194) of human eNOS.
Epitope
SLQERQLRGAVPWAFD
Specificity
This does not detect human inducible NOS or rat brain NOS.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOS3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 1:50-1:1000
Application Notes
In IHC, antigen retrieval is not essential but may optimize staining. By Western blot, a 140kDa band is seen in human endothelial cells. Optimal dilutions should be determined by the end user.
Theoretical MW
133 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB300-500 in the following applications:

Publications
Read Publications using
NB300-500 in the following applications:

  • 1 publication
  • 3 publications
  • WB
    11 publications

Reactivity Notes

Sheep reactivity reported in scientific literature (PMID: 31352175).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for eNOS Antibody - BSA Free

  • Constitutive NOS
  • EC 1.14.13.39
  • ECNOS
  • Endothelial NOS
  • endothelial
  • eNOS
  • eNOScNOS
  • nitric oxide synthase 3 (endothelial cell)
  • NOS type III
  • NOS3
  • NOSIII

Background

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for eNOS Antibody (NB300-500)(24)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Porcine, Sheep.

We have publications tested in 3 applications: FLOW, IF/IHC, WB.


Filter By Application
FLOW
(1)
IF/IHC
(3)
WB
(11)
All Applications
Filter By Species
Human
(2)
Mouse
(5)
Rat
(3)
Porcine
(1)
Sheep
(2)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using NB300-500 Applications Species
Cosarderelioglu C, Kreimer S, Plaza-Rodriguez AI et al. Decoding Angiotensin Receptors: TOMAHAQ-Based Detection and Quantification of Angiotensin Type-1 and Type-2 Receptors Journal of the American Heart Association 2023-09-19 [PMID: 37681524] (WB) WB
Lee S, Baek S, Lee Y et al. Ginsenoside Rg3-enriched Korean red ginseng extract attenuates Non-Alcoholic Fatty Liver Disease by way of suppressed VCAM-1 expression in liver sinusoidal endothelium Journal of Ginseng Research 2022-10-01 [PMID: 37252282] (FLOW, Mouse) FLOW Mouse
SuSnjara P, Mihaljevic Z, Stupin A et al. Consumption of nutritionally enriched hen eggs enhances endothelium-dependent vasodilation via cyclooygenases� metabolites in healthy young persons - A randomized study Research Square 2022-11-02 [PMID: 37049437] (WB, Human) WB Human
Fujita M, Tanaka N, Sakaguchi Y et al. Small caliber heparin loaded ultrafine microfiber woven graft achieved high patency rate in a preliminary study of canine carotid artery implantation JVS Vasc Sci 2021-10-07 [PMID: 34617041]
Patel PD, Chen YL, Kasetti RB et al. Impaired TRPV4-eNOS signaling in trabecular meshwork elevates intraocular pressure in glaucoma Proceedings of the National Academy of Sciences of the United States of America 2021-04-20 [PMID: 33853948]
Ricardo-da-Silva FY, Armstrong R, Vidal-Dos-Santos M Et al. 17 beta-Estradiol Treatment Protects Lungs Against Brain Death Effects in Female Rat Donor Transplantation 2021-04-01 [PMID: 33031230]
Ottolini M, Daneva Z, Chen YL et al. Mechanisms underlying selective coupling of endothelial Ca2+ signals with eNOS versus IK/SK channels in systemic and pulmonary arteries J. Physiol. (Lond.) 2020-05-28 [PMID: 32463112] (WB) WB
Patel G, Fury W, Yang H et al. Molecular taxonomy of human ocular outflow tissues defined by single cell transcriptomics Proc Natl Acad Sci U S A 2020-05-23 [PMID: 32439707]
Sadhukhan R, Leung J. W. C, et al. Fractionated radiation suppresses Kruppel-like factor 2 pathway to a greater extent than by single exposure to the same total dose. Sci Rep 2020-05-07 [PMID: 32382091] (WB, Human) WB Human
Gao XX, Zhang QF, Zhu M et al. Effects of l-arginine on endometrial estrogen receptor alpha/beta and progesterone receptor expression in nutrient-restricted sheep Theriogenology 2019-07-25 [PMID: 31352175] (WB, Sheep) WB Sheep
Show All 24 Publications.

Reviews for eNOS Antibody (NB300-500) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using NB300-500:
Filter by Applications
WB
(1)
IHC
(1)
IHC-P
(1)
All Applications
Filter by Species
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
WB Mouse 05/23/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse brain
SpeciesMouse
  5
reviewed by:
Verified Customer
IHC Mouse 05/23/2017
View

Summary

ApplicationImmunohistochemistry
Sample TestedMouse brain
SpeciesMouse
Immunohistochemistry-Paraffin eNOS NB300-500
Enlarge
5
reviewed by:
Verified Customer
IHC-P Mouse 02/22/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Carotid Artery
SpeciesMouse

Product General Protocols

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Video Protocols

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FAQs for eNOS Antibody (NB300-500) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

 
Verified Customer
05/23/2017
Application: WB
Species: Mouse

 
Verified Customer
05/23/2017
Application: IHC
Species: Mouse

Verified Customer
02/22/2016
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol NOS3
Entrez
Uniprot