eNOS Antibody

Immunohistochemistry: eNOS Antibody [NB300-500] - The pulmonary tissues were analyzed for expression of eNOS protein by immunohistochemistry(IHC). Dark brown staining of indicates eNOS-positive cells. IHC stain of representative pulmonary artery endothelial cells from rats subjected to CON=Control Group, ALI=Acute lung injury Group, LV=Low tidal volume Group, VLV=Very low tidal volume Group, MV=Large tidal volume Group (magnification, x400).

Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Staining of eNOS in human lung tissue.

Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Mouse Carotid Artery stained with eNOS antibody. Image from verified customer review.

• Reactivity: Hu, Mu, Rt, Po, Am, Bv, Ca, Dr
• Applications: WB, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

eNOS Antibody Summary

• Immunogen: Synthetic peptide corresponding to residues S(1179) L Q E R Q L R G A V P W A F D(1194) of human eNOS.
• Epitope: SLQERQLRGAVPWAFD
• Specificity: This does not detect human inducible NOS or rat brain NOS.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NOS3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:100
• Application Notes: This antibody can be used in WB and Immunohistochemistry. In IHC, antigen retrieval is not essential but may optimise staining. By WB, a 140kDa band is seen in human endothelial cells. Recommended Starting Dilutions: IHC-P: 1/100 WB: 1/1000 Not tested in other applications. *Optimal dilutions should be determined by the end user.
• Theoretical MW: 133 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 27496502). Mouse reactivity reported in scientific literature (PMID: 25830942).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.6) and 1mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for eNOS Antibody

• Constitutive NOS
• EC 1.14.13.39
• ECNOS
• Endothelial NOS
• endothelial
• eNOS
• eNOScNOS
• nitric oxide synthase 3 (endothelial cell)
• NOS type III
• NOS3
• NOSIII

Background

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for eNOS Antibody (NB300-500)(10)

Publications using NB300-500

• Zhang L, Zheng H, Luo J et al. Inhibition of endothelial nitric oxide synthase reverses the effect of exercise on improving cognitive function in hypertensive rats. Hypertens. Res. Mar 22 2018 [PMID: 29568075] (IHC, Rat)
• Leao VF, Ferreira LLDM, Melo CM, Bonfleur ML. Taurine supplementation prevents endothelial dysfunction and attenuates structural changes in aortas from hypothalamic obese rats. Eur J Nutr. 2018 Jan 27 [PMID: 29374793] (WB, Rat)
• Nelson RK, Ya-Ping J, Gadbery J et al. Phospholipase D2 loss results in increased blood pressure via inhibition of the endothelial nitric oxide synthase pathway Sci Rep 2017 Aug 22 [PMID: 28831159] (WB, Mouse)
• Oczkowicz G, Caban A, Budzinski G et al. Endothelial Nitric Oxide Synthase and Endothelin-1 Expression in the Early Post-Porcine Kidney Autotransplantation Period. Transplant. Proc. Jun 1 2016 [PMID: 27496502] (WB, Porcine)
• Kurobe H, Maxfield MW, Tara S et al. Development of small diameter nanofiber tissue engineered arterial grafts PLoS ONE 2015 Apr 02 [PMID: 25830942] (IHC, Mouse)
• Oh YK, Bachar AR, Zacharias DG et al. Humanin Preserves Endothelial Function and Prevents Atherosclerotic Plaque Progression in Hypercholesterolemic ApoE Deficient Mice. Atherosclerosis;219(1):65-73. 2011 Nov. [PMID: 21763658]
• HOGBEN L. Risk of jaundice following transfusion with pooled plasma or serum. Br J Soc Med 1947 Jul [PMID: 20253608]
• Shirasuna K, Asahi T, Sasaki M et al. Distribution of arteriolovenous vessels, capillaries and eNOS expression in the bovine corpus luteum during the estrous cycle: a possible implication of different sensitivity by luteal phase to PGF(2alpha) in the increase of luteal blood flow. J Reprod Dev. 2010 Feb. [PMID: 19893278]
• Wang M, Lung MA. Acetylcholine induces contractile and relaxant effects in canine nasal venous systems. Eur Respir J. 2006 Oct. [PMID: 16737988]
• Guo FH, Comhair SA, Zheng S et al. Molecular mechanisms of increased nitric oxide (NO) in asthma: evidence for transcriptional and post-translational regulation of NO synthesis. J Immunol. 2000 Jun. [PMID: 10820280]

Reviews for eNOS Antibody (NB300-500) (3)

Average Rating: 4.7

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Mouse.

reviewed by: Anonymous WB Mouse 05/23/2017

Summary

• Application: Western Blot
• Sample Tested: Mouse brain
• Species: Mouse

reviewed by: Anonymous IHC Mouse 05/23/2017

Summary

• Application: Immunohistochemistry
• Sample Tested: Mouse brain
• Species: Mouse

reviewed by: Anonymous IHC-P Mouse 02/22/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse Carotid Artery
• Species: Mouse

Bioinformatics

• Gene Symbol: NOS3
• Entrez:
  • 4846(Human)
  • 18127(Mouse)
  • 24600(Rat)
• Uniprot:
  • P29474()