Myeloperoxidase/MPO Antibody [Unconjugated]

Myeloperoxidase/MPO was detected in immersion fixed paraffin-embedded sections of mouse spleen tissue using Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents.For human Immunohistochemistry samples, we recommend Mouse Anti-Human Myeloperoxidase/ MPO Monoclonal Antibody (MAB3174).

Western blot shows lysates of HL‑60 human acute promyelocytic leukemia cell line, human neutrophil cells, and mouse spleen tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Myeloperoxidase/MPO at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for Myeloperoxidase/MPO at approximately 67 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

Myeloperoxidase/MPO was detected in immersion fixed mouse splenocytes using Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Myeloperoxidase/MPO Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse MPO
  Met16-Thr718
  Accession # P11247
• Specificity: Detects human and mouse Myeloperoxidase/MPO in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse EPPO is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MPO
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Myeloperoxidase/MPO Antibody [Unconjugated]

• EC 1.11.1
• EC 1.11.1.7
• MPO
• Myeloperoxidase

Background

Myeloperoxidase (MPO) is a hemeprotein that belongs to the XPO subfamily of the heme peroxidase superfamily. MPO is synthesized as a preproprotein that undergoes proteolytic processing to generate a disulfide-linked heterodimer of the N-terminal beta -subunit (12 kDa) and C-terminal alpha subunit (60 kDa). Active MPO is a tetramer of two beta -subunits and two alpha -subunits that are also disulfide-linked through the two alpha -subunits. MPO is stored in granules and is an abundant protein in neutrophils and monocytes. MPO is released upon activation to catalyze the formation of powerful oxidants such as hypochlorous acid, which kills microbes. Unprocessed pro-MPO can also be released. Mouse MPO shares 87% amino acid sequence identity with that of human MPO.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myeloperoxidase/MPO Antibody (AF3667)(66)

Publications using AF3667

• K Wang, Y Liao, X Li, R Wang, Z Zeng, M Cheng, L Gao, D Xu, F Wen, T Wang, J Chen Inhibition of neutrophil elastase prevents cigarette smoke exposure-induced formation of neutrophil extracellular traps and improves lung function in a mouse model of chronic obstructive pulmonary disease International immunopharmacology, 2022;114(0):109537. 2022 [PMID: 36495695] (ICC, Human)
• J Huang, H Tao, PG Yancey, Z Leuthner, LS May-Zhang, JY Jung, Y Zhang, L Ding, V Amarnath, D Liu, S Collins, SS Davies, MF Linton Scavenging dicarbonyls with 5'-O-pentyl-pyridoxamine increases HDL net cholesterol efflux capacity and attenuates atherosclerosis and insulin resistance Molecular Metabolism, 2022;67(0):101651. 2022 [PMID: 36481344] (IHC, Mouse)
• J Zeller, KS Cheung Tun, TL Nero, JD McFadyen, G Krippner, B Bogner, S Kreuzaler, J Kiefer, VK Horner, D Braig, H Danish, S Baratchi, M Fricke, X Wang, MG Kather, B Kammerer, KJ Woollard, P Sharma, CJ Morton, G Pietersz, MW Parker, K Peter, SU Eisenhardt A novel phosphocholine-mimetic inhibits a pro-inflammatory conformational change in C-reactive protein Embo Molecular Medicine, 2022;0(0):e16236. 2022 [PMID: 36468184] (ICC, Human)
• J Yang, L Jin, HS Kim, F Tian, Z Yi, K Bedi, M Ljungman, M Pasca di M, H Crawford, J Shi KDM6A Loss Recruits Tumor-Associated Neutrophils and Promotes Neutrophil Extracellular Trap Formation in Pancreatic Cancer Cancer Research, 2022;0(0):OF1-OF14. 2022 [PMID: 36306422] (ICC, Human)
• PS Sung, YC Peng, SP Yang, CH Chiu, SL Hsieh CLEC5A is critical in Pseudomonas aeruginosa-induced acute lung injury JCI Insight, 2022;0(0):. 2022 [PMID: 36048544] (IHC, Mouse)
• H Li, C Qiao, L Zhao, Q Jing, D Xue, Y Zhang Epigallocatechin-3-gallate reduces neutrophil extracellular trap formation and tissue injury in severe acute pancreatitis Journal of leukocyte biology, 2022;0(0):. 2022 [PMID: 35983712] (ICC, Human)
• A Gupta, K Singh, S Fatima, S Ambreen, S Zimmermann, R Younis, S Krishnan, R Rana, I Gadi, C Schwab, R Biemann, K Shahzad, V Rani, S Ali, PR Mertens, S Kohli, B Isermann Neutrophil Extracellular Traps Promote NLRP3 Inflammasome Activation and Glomerular Endothelial Dysfunction in Diabetic Kidney Disease Nutrients, 2022;14(14):. 2022 [PMID: 35889923] (IHC, Mouse)
• W Chen, B Li, F Jia, J Li, H Huang, C Ni, W Xia High PANX1 Expression Leads to Neutrophil Recruitment and the Formation of a High Adenosine Immunosuppressive Tumor Microenvironment in Basal-like Breast Cancer Cancers, 2022;14(14):. 2022 [PMID: 35884429] (IHC, Human)
• N Caporarell, J Lee, TX Pham, DL Jones, J Guan, PA Link, JA Meridew, G Marden, T Yamashita, CA Osborne, AV Bhagwate, SK Huang, RF Nicosia, DJ Tschumperl, M Trojanowsk, G Ligresti Dysfunctional ERG signaling drives pulmonary vascular aging and persistent fibrosis Nature Communications, 2022;13(1):4170. 2022 [PMID: 35879310] (IHC, Mouse)
• NPK Le, AF do Nascime, D Schneberge, CC Quach, X Zhang, GK Aulakh, W Dawicki, L Liu, JR Gordon, B Singh Deficiency of leukocyte-specific protein 1 (LSP1) alleviates asthmatic inflammation in a mouse model Respiratory Research, 2022;23(1):165. 2022 [PMID: 35733161] (IHC, Mouse)
• D Ye, J Yao, W Du, C Chen, Y Yang, K Yan, J Li, Y Xu, S Zang, Y Zhang, X Rong, R Zhang, A Xu, J Guo Neutrophil Extracellular Traps Mediate Acute Liver Failure in Regulation of miR-223/neutrophil Elastase Signaling in Mice Cellular and Molecular Gastroenterology and Hepatology, 2022;0(0):. 2022 [PMID: 35660025] (Western Blot, Mouse)
• C Arnholdt, K Kumaraswam, P Götz, M Kübler, M Lasch, E Deindl Depletion of gammadelta T Cells Leads to Reduced Angiogenesis and Increased Infiltration of Inflammatory M1-like Macrophages in Ischemic Muscle Tissue Cells, 2022;11(9):. 2022 [PMID: 35563796] (IHC, Mouse)
• H Wang, DJ Tumes, TR Hercus, KH Yip, C Aloe, R Vlahos, AF Lopez, N Wilson, CM Owczarek, S Bozinovski Blocking the human common beta subunit of the GM-CSF, IL-5 and IL-3 receptors markedly reduces hyperinflammation in ARDS models Cell Death & Disease, 2022;13(2):137. 2022 [PMID: 35145069] (IHC, Mouse)
• JM Adrover, L Carrau, J Da beta ler-Ple, Y Bram, V Chandar, S Houghton, D Redmond, JR Merrill, M Shevik, BR tenOever, SK Lyons, RE Schwartz, M Egeblad Disulfiram inhibits neutrophil extracellular trap formation protecting rodents from acute lung injury and SARS-CoV-2 infection JCI Insight, 2022;0(0):. 2022 [PMID: 35133984] (IHC, Hamster)
• C Conrad, D Yildiz, SJ Cleary, A Margraf, L Cook, U Schlomann, B Panaretou, JL Bowser, H Karmouty-Q, J Li, NK Berg, SC Martin, A Aljohmani, SF Moussavi-H, KM Wang, JJ Tian, M Magnen, C Valet, L Qiu, JP Singer, HK Eltzschig, CAPSys Stu, W Bertrams, S Herold, N Suttorp, B Schmeck, ZT Ball, A Zarbock, MR Looney, JW Bartsch ADAM8 signaling drives neutrophil migration and ARDS severity JCI Insight, 2022;7(3):. 2022 [PMID: 35132956] (IHC, Mouse)
• KC Ngamsri, RA Putri, C Jans, K Schindler, A Fuhr, Y Zhang, J Gamper-Tsi, S Ehnert, FM Konrad CXCR4 and CXCR7 Inhibition Ameliorates the Formation of Platelet-Neutrophil Complexes and Neutrophil Extracellular Traps through Adora2b Signaling International Journal of Molecular Sciences, 2021;22(24):. 2021 [PMID: 34948374] (ICC, Human)
• W Cao, MY Zhu, SH Lee, SB Lee, HJ Kim, BO Park, CH Yoon, D Khadka, GS Oh, H Shim, TH Kwak, HS So Modulation of Cellular NAD+ Attenuates Cancer-Associated Hypercoagulability and Thrombosis via the Inhibition of Tissue Factor and Formation of Neutrophil Extracellular Traps International Journal of Molecular Sciences, 2021;22(21):. 2021 [PMID: 34769515] (IHC, Mouse)
• Y Lu, Y Huang, J Li, J Huang, L Zhang, J Feng, J Li, Q Xia, Q Zhao, L Huang, S Jiang, S Su Eosinophil extracellular traps drive asthma progression through neuro-immune signals Nature Cell Biology, 2021;23(10):1060-1072. 2021 [PMID: 34616019] (ICC, Mouse)
• F Harnischfe, F O'Connell, M Weiss, B Axelrod, A Hajnal, K Czaja, PM Di Lorenzo, R Dando Sprague Dawley Rats Gaining Weight on a High Energy Diet Exhibit Damage to Taste Tissue Even after Return to a Healthy Diet Nutrients, 2021;13(9):. 2021 [PMID: 34578940] (IHC, Rat)
• S Arandjelov, JSA Perry, M Zhou, A Ceroi, I Smirnov, SF Walk, LS Shankman, I Cambré, S Onengut-Gu, D Elewaut, TP Conrads, KS Ravichandr ELMO1 signaling is a promoter of osteoclast function and bone loss Nature Communications, 2021;12(1):4974. 2021 [PMID: 34404802] (Western Blot, Mouse)
• D Dora, S Ferenczi, R Stavely, VE Toth, ZV Varga, T Kovacs, I Bodi, R Hotta, KJ Kovacs, AM Goldstein, N Nagy Evidence of a Myenteric Plexus Barrier and Its Macrophage-Dependent Degradation During Murine Colitis: Implications in Enteric Neuroinflammation Cellular and Molecular Gastroenterology and Hepatology, 2021;0(0):. 2021 [PMID: 34246810] (IHC, Mouse)
• N Belforte, J Agostinone, L Alarcon-Ma, D Villafranc, F Dotigny, JL Cueva Varg, A Di Polo AMPK hyperactivation promotes dendrite retraction, synaptic loss, and neuronal dysfunction in glaucoma Molecular Neurodegeneration, 2021;16(1):43. 2021 [PMID: 34187514] (IHC, Mouse)
• R Surolia, FJ Li, Z Wang, M Kashyap, RK Srivastava, AM Traylor, P Singh, KG Dsouza, H Kim, JF Pittet, JW Zmijewski, A Agarwal, M Athar, A Ahmad, VB Antony NETosis in the pathogenesis of acute lung injury following cutaneous chemical burns JCI Insight, 2021;6(10):. 2021 [PMID: 34027893] (ICC, Human)
• M Kübler, S Beck, S Fischer, P Götz, K Kumaraswam, H Ishikawa-A, M Lasch, E Deindl Absence of Cold-Inducible RNA-Binding Protein (CIRP) Promotes Angiogenesis and Regeneration of Ischemic Tissue by Inducing M2-Like Macrophage Polarization Biomedicines, 2021;9(4):. 2021 [PMID: 33916904] (IHC, Mouse)
• L Klemke, T De Oliveir, D Witt, N Winkler, H Bohnenberg, R Bucala, LC Conradi, R Schulz-Hed Hsp90-stabilized MIF supports tumor progression via macrophage recruitment and angiogenesis in colorectal cancer Cell Death & Disease, 2021;12(2):155. 2021 [PMID: 33542244] (IHC, Mouse)
• R Mehling, J Schwenck, C Lemberg, C Trautwein, L Zizmare, D Kramer, A Müller, B Fehrenbach, I Gonzalez-M, L Quintanill, K Schröder, RP Brandes, M Schaller, W Ruf, M Eichner, K Ghoreschi, M Röcken, BJ Pichler, M Kneilling Immunomodulatory role of reactive oxygen species and nitrogen species during T cell-driven neutrophil-enriched acute and chronic cutaneous delayed-type hypersensitivity reactions Theranostics, 2021;11(2):470-490. 2021 [PMID: 33391487] (IHC, Mouse)
• MJ Thomas, E Major, A Benedek, I Horváth, D Máthé, R Bergmann, AM Szász, T Krenács, Z Benyó Suppression of Metastatic Melanoma Growth in Lung by Modulated Electro-Hyperthermia Monitored by a Minimally Invasive Heat Stress Testing Approach in Mice Cancers, 2020;12(12):. 2020 [PMID: 33371498] (IHC, Mouse)
• L Moreira-Te, PJ Stimpson, E Stavropoul, S Hadebe, P Chakravart, M Ioannou, IV Aramburu, E Herbert, SL Priestnall, A Suarez-Bon, J Sousa, KL Fonseca, Q Wang, S Vashakidze, P Rodríguez-, C Vilaplana, M Saraiva, V Papayannop, A O'Garra Type I IFN exacerbates disease in tuberculosis-susceptible mice by inducing neutrophil-mediated lung inflammation and NETosis Nat Commun, 2020;11(1):5566. 2020 [PMID: 33149141] (ICC, Mouse)
• F Essig, AM Kollikowsk, M Pham, L Solymosi, G Stoll, KG Haeusler, P Kraft, MK Schuhmann Immunohistological Analysis of Neutrophils and Neutrophil Extracellular Traps in Human Thrombemboli Causing Acute Ischemic Stroke Int J Mol Sci, 2020;21(19):. 2020 [PMID: 33036337] (IHC, Human)
• WJ Ouwendijk, HJ van den Ha, MW Delany, JJ van Kampen, GP van Nierop, T Mehraban, F Zaaraoui-B, WF van IJcken, JM van den Br, RD De Vries, AC Andeweg, GM Verjans Alveolar barrier disruption in varicella pneumonia is associated with neutrophil extracellular trap formation JCI Insight, 2020;0(0):. 2020 [PMID: 33021967] (IHC, Primate)
• S Taylor, M Whitfield, J Barratt, A Didangelos The Metalloproteinase ADAMTS5 Is Expressed by Interstitial Inflammatory Cells in IgA Nephropathy and Is Proteolytically Active on the Kidney Matrix J. Immunol., 2020;0(0):. 2020 [PMID: 32917786] (Western Blot, Human)
• EY Lin, HJ Lai, YK Cheng, KQ Leong, LC Cheng, YC Chou, YC Peng, YH Hsu, HS Chiang Neutrophil Extracellular Traps Impair Intestinal Barrier Function during Experimental Colitis Biomedicines, 2020;8(8):. 2020 [PMID: 32764411] (ELISA Capture, Mouse)
• EY Lin, HJ Lai, YK Cheng, KQ Leong, LC Cheng, YC Chou, YC Peng, YH Hsu, HS Chiang Neutrophil Extracellular Traps Impair Intestinal Barrier Function during Experimental Colitis Biomedicines, 2020;8(8):. 2020 [PMID: 32764411] (ELISA Capture, Mouse)
• K Vaibhav, M Braun, K Alverson, H Khodadadi, A Kutiyanawa, A Ward, C Banerjee, T Sparks, A Malik, MH Rashid, MB Khan, MF Waters, DC Hess, AS Arbab, JR Vender, N Hoda, B Baban, KM Dhandapani Neutrophil extracellular traps exacerbate neurological deficits after traumatic brain injury Sci Adv, 2020;6(22):eaax8847. 2020 [PMID: 32523980] (ICC, Mouse)
• TD Tsouroukts, A Warnatsch, M Ioannou, D Hoving, Q Wang, V Papayannop Histones, DNA, and Citrullination Promote Neutrophil Extracellular Trap Inflammation by Regulating the Localization and Activation of TLR4 Cell Rep, 2020;31(5):107602. 2020 [PMID: 32375035] (Western Blot, Human)
• RGS Chirivi, JWG van Rosmal, M van der Li, M Euler, G Schmets, G Bogatkevic, K Kambas, J Hahn, Q Braster, O Soehnlein, MH Hoffmann, HHGV Es, JMH Raats Therapeutic ACPA inhibits NET formation: a potential therapy for neutrophil-mediated inflammatory diseases Cell. Mol. Immunol., 2020;0(0):. 2020 [PMID: 32203195] (IHC, Mouse)
• KL Fonseca, AR Maceiras, R Matos, L Simoes-Cos, J Sousa, B Cá, L Barros, AI Fernandes, S Mereiter, R Reis, J Gomes, G Tapia, P Rodríguez-, M Martín-Cés, S Vashakidze, S Gogishvili, K Nikolaishv, R Appelberg, F Gärtner, PNS Rodrigues, C Vilaplana, CA Reis, A Magalhães, M Saraiva Deficiency in the glycosyltransferase Gcnt1 increases susceptibility to tuberculosis through a mechanism involving neutrophils Mucosal Immunol, 2020;0(0):. 2020 [PMID: 32203062] (IHC, Mouse)
• JM Adrover, A Aroca-Crev, G Crainiciuc, F Ostos, Y Rojas-Vega, A Rubio-Ponc, C Cilloniz, E Bonzón-Kul, E Calvo, D Rico, MA Moro, C Weber, I Lizasoaín, A Torres, J Ruiz-Cabel, J Vázquez, A Hidalgo Programmed 'disarming' of the neutrophil proteome reduces the magnitude of inflammation Nat. Immunol., 2020;21(2):135-144. 2020 [PMID: 31932813] (Functional Assay, Human)
• J Endig, L Unrau, P Sprezyna, S Rading, M Karsak, D Goltz, LC Heukamp, G Tiegs, L Diehl Acute Liver Injury after CCl4 Administration is Independent of Smad7 Expression in Myeloid Cells Int J Mol Sci, 2019;20(22):. 2019 [PMID: 31698731] (IHC, Mouse)
• S Lorscheid, A Müller, J Löffler, C Resch, P Bucher, FC Kurschus, A Waisman, K Schäkel, S Hailfinger, K Schulze-Os, D Kramer Keratinocyte-derived I&kappaB? drives psoriasis and associated systemic inflammation JCI Insight, 2019;4(22):. 2019 [PMID: 31622280] (IHC, Mouse)
• C Radermecke, C Sabatel, C Vanwinge, C Ruscitti, P Maréchal, F Perin, J Schyns, N Rocks, M Toussaint, D Cataldo, SL Johnston, F Bureau, T Marichal Locally instructed CXCR4hi neutrophils trigger environment-driven allergic asthma through the release of neutrophil extracellular traps Nat. Immunol., 2019;0(0):. 2019 [PMID: 31591573] (ICC, Mouse)
• K Bitschar, B Sauer, J Focken, H Dehmer, S Moos, M Konnerth, NA Schilling, S Grond, H Kalbacher, FC Kurschus, F Götz, B Krismer, A Peschel, B Schittek Lugdunin amplifies innate immune responses in the skin in synergy with host- and microbiota-derived factors Nat Commun, 2019;10(1):2730. 2019 [PMID: 31227691] (IHC, Mouse)
• PS Sung, TF Huang, SL Hsieh Extracellular vesicles from CLEC2-activated platelets enhance dengue virus-induced lethality via CLEC5A/TLR2 Nat Commun, 2019;10(1):2402. 2019 [PMID: 31160588] (ICC, Human)
• BM Szczerba, F Castro-Gin, M Vetter, I Krol, S Gkountela, J Landin, MC Scheidmann, C Donato, R Scherrer, J Singer, C Beisel, C Kurzeder, V Heinzelman, C Rochlitz, WP Weber, N Beerenwink, N Aceto Neutrophils escort circulating tumour cells to enable cell cycle progression Nature, 2019;0(0):. 2019 [PMID: 30728496] (ICC, Mouse)
• N Simon, A Antignani, SM Hewitt, M Gadina, C Alewine, D FitzGerald Tofacitinib enhances delivery of antibody-based therapeutics to tumor cells through modulation of inflammatory cells JCI Insight, 2019;0(0):. 2019 [PMID: 30720466] (IHC-P, Xenograft)
• Z Zeng, Y Zhang, X Liang, F Wang, J Zhao, Z Xu, X Liu, X Liu Qingnao dripping pills mediate immune-inflammatory response and MAPK signaling pathway after acute ischemic stroke in rats J. Pharmacol. Sci., 2019;0(0):. 2019 [PMID: 30713113] (Western Blot, Rat)
• J Rojas-Quin, X Wang, J Tipper, PR Burkett, J Zuñiga, AR Ashtekar, F Polverino, A Rout, I Yambayev, C Hernández, L Jimenez, G Ramírez, KS Harrod, CA Owen Matrix metalloproteinase-9 deficiency protects mice from severe influenza A viral infection JCI Insight, 2018;3(24):. 2018 [PMID: 30568032] (IHC, Mouse)
• S Offenbache, Y Jiao, SJ Kim, J Marchesan, KL Moss, L Jing, K Divaris, S Bencharit, CS Agler, T Morelli, S Zhang, L Sun, WT Seaman, D Cowley, SP Barros, JD Beck, M Munz, AS Schaefer, KE North GWAS for Interleukin-1? levels in gingival crevicular fluid identifies IL37 variants in periodontal inflammation Nat Commun, 2018;9(1):3686. 2018 [PMID: 30206230] (IHC, Mouse)
• Z Cheng, L Wang, M Qu, H Liang, W Li, Y Li, L Deng, Z Zhang, GY Yang Mesenchymal stem cells attenuate blood-brain barrier leakage after cerebral ischemia in mice J Neuroinflammation, 2018;15(1):135. 2018 [PMID: 29724240] (IHC, Mouse)
• H Lörchner, C Widera, Y Hou, A Elsässer, H Warnecke, E Giannitsis, JS Hulot, T Braun, KC Wollert, J Pöling Reg3? is associated with cardiac inflammation and provides prognostic information in patients with acute coronary syndrome Int. J. Cardiol., 2018;258(0):7-13. 2018 [PMID: 29544958] (Western Blot, Mouse)
• SY Cheon, JM Kim, EH Kam, CC Ho, EJ Kim, S Chung, JH Jeong, DD Lee, SW Lee, BN Koo Cell-penetrating interactomic inhibition of nuclear factor-kappa B in a mouse model of postoperative cognitive dysfunction Sci Rep, 2017;7(1):13482. 2017 [PMID: 29044209] (IHC-Fr, Mouse)
• MB Johnson, B Pang, DJ Gardner, S Niknam-Ben, V Soundaraja, A Bramos, DP Perrault, K Banks, GK Lee, RY Baker, GH Kim, S Lee, Y Chai, M Chen, W Li, L Kwong, YK Hong, AK Wong Topical Fibronectin Improves Wound Healing of Irradiated Skin Sci Rep, 2017;7(1):3876. 2017 [PMID: 28634413] (IHC, Mouse)
• CWL Dougher, A Buffone, MJ Nemeth, M Nasirikena, EE Irons, PN Bogner, JTY Lau The blood-borne sialyltransferase ST6Gal-1 is a negative systemic regulator of granulopoiesis J. Leukoc. Biol., 2017;0(0):. 2017 [PMID: 28550122] (Western Blot, Mouse)
• M Toussaint, DJ Jackson, D Swieboda, A Guedán, TD Tsouroukts, YM Ching, C Radermecke, H Makrinioti, J Aniscenko, MR Edwards, R Solari, F Farnir, V Papayannop, F Bureau, T Marichal, SL Johnston Host DNA released by NETosis promotes rhinovirus-induced type-2 allergic asthma exacerbation Nat. Med., 2017;23(6):681-691. 2017 [PMID: 28459437] (IHC, Mouse)
• FM Silva, EE Oliveira, AC Gouveia, AS Brugiolo, CC Alves, JO Correa, J Gameiro, J Mattes, HC Teixeira, AP Ferreira Obesity promotes prolonged ovalbumin-induced airway inflammation modulating Th1, Th2 and Th17 immune responses in BALB/c mice Clin. Exp. Immunol, 2017;0(0):. 2017 [PMID: 28263381] (IHC, Mouse)
• Luise Erpenbeck PAD4 Deficiency Decreases Inflammation and Susceptibility to Pregnancy Loss in a Mouse Model Biol. Reprod, 2016;95(6):132. 2016 [PMID: 28007693] (IHC-P, Mouse)
• Swimming Motility Mediates the Formation of Neutrophil Extracellular Traps Induced by Flagellated Pseudomonas aeruginosa PLoS Pathog., 2016;12(11):e1005987. 2016 [PMID: 27855208] (IHC, Mouse)
• Beneficial Effects of Sodium Phenylbutyrate Administration During Infection with Salmonella enterica serovar Typhimurium Infect Immun, 2016;0(0):. 2016 [PMID: 27382022] (Western Blot, Mouse)
• Neutrophil Attack Triggers Extracellular Trap-Dependent Candida Cell Wall Remodeling and Altered Immune Recognition PLoS Pathog, 2016;12(5):e1005644. 2016 [PMID: 27223610] (IHC, Mouse)
• Lawler C, Milho R, May J, Stevenson P Rhadinovirus host entry by co-operative infection. PLoS Pathog, 2015;11(3):e1004761. 2015 [PMID: 25790477] (IHC, Mouse)
• Correction: A chitin-like component on sclerotic cells of fonsecaea pedrosoi inhibits dectin-1-mediated murine Th17 development by masking beta-glucans. PLoS ONE, 2015;10(3):e0119244. 2015 [PMID: 25786219] (Western Blot, Mouse)
• Khailova L, Petrie B, Baird C, Dominguez Rieg J, Wischmeyer P Lactobacillus rhamnosus GG and Bifidobacterium longum attenuate lung injury and inflammatory response in experimental sepsis. PLoS ONE, 2014;9(5):e97861. 2014 [PMID: 24830455] (IHC, Mouse)
• Behnsen J, Jellbauer S, Wong C, Edwards R, George M, Ouyang W, Raffatellu M The cytokine IL-22 promotes pathogen colonization by suppressing related commensal bacteria. Immunity, 2014;40(2):262-73. 2014 [PMID: 24508234] (Western Blot, Mouse)
• Guabiraba R, Besnard A, Menezes G, Secher T, Jabir M, Amaral S, Braun H, Lima-Junior R, Ribeiro R, Cunha F, Teixeira M, Beyaert R, Graham G, Liew F IL-33 targeting attenuates intestinal mucositis and enhances effective tumor chemotherapy in mice. Mucosal Immunol, 2014;7(5):1079-93. 2014 [PMID: 24424522] (IHC, Mouse)
• Le Goffic R, Leymarie O, Chevalier C, Rebours E, Da Costa B, Vidic J, Descamps D, Sallenave JM, Rauch M, Samson M, Delmas B Transcriptomic analysis of host immune and cell death responses associated with the influenza A virus PB1-F2 protein. PLoS Pathog., 2011;7(8):e1002202. 2011 [PMID: 21901097] (IHC-Fr, Mouse)
• Gill SE, Huizar I, Bench EM Tissue inhibitor of metalloproteinases 3 regulates resolution of inflammation following acute lung injury. Am. J. Pathol., 2010;176(1):64-73. 2010 [PMID: 20008147] (ELISA Development, Mouse)

Reviews for Myeloperoxidase/MPO Antibody (AF3667) (3)

Average Rating: 4.7

(Based on 3 reviews)

We have 3 reviews tested in 2 species: Human, Rabbit.

reviewed by: Aida Meghraoui Imaging Mass Cytometry (IMC) Human 10/05/2020

Summary

• Application: Other
• Sample Tested: FFPE
• Species: Human
• Lot: YBZ0319051

reviewed by: Christian Schuerch Immunofluorescence - paraffin Human 04/12/2019

Summary

• Application: Other
• Sample Tested: human tonsil
• Species: Human
• Lot: I don't have it any more

Comments

• Comments: pH9 antigen retrieval

reviewed by: Andrea Puhar IHC-P Rabbit 01/11/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Small intestine
• Species: Rabbit
• Lot: YBZ0216021

Comments

• Comments: We have tested the antibody for use in rabbit.
  PFA-fixed ileum embedded in paraffin was used, sections were 7 micrometer thick. Details about antigen retrieval are available upon request and outlined in the attached image.
  We also performed WB analysis with these rabbit samples. Image available upon request.
  Before trying with rabbit, we used samples from mouse (previously tested species) to confirm that the antibody is working in our hands. Details available upon request.
  Please don't hesitte to contact us in case of questions.
  We woud like to participate in the Innovator Reward. Thanks.

Bioinformatics

• Gene Symbol: MPO
• Uniprot:
  • P11247(Mouse)