Myeloperoxidase/MPO Antibody [Unconjugated]

Images

 
Western blot shows lysates of HL‑60 human acute promyelocytic leukemia cell line, human neutrophil cells, and mouse spleen tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Myeloperoxidase/MPO ...read more
Myeloperoxidase/MPO was detected in immersion fixed mouse splenocytes using Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) at 15 µg/mL for 3 hours at room ...read more
Myeloperoxidase/MPO was detected in immersion fixed paraffin-embedded sections of mouse spleen tissue using Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) at ...read more
Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for Myeloperoxidase/MPO at approximately 67 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse ...read more
SAA levels positively correlate with tumour-associated neutrophil infiltration. (A) Representative images of MPO-positive neutrophils in a lung adenocarcinoma tumour section (original magnification ×200). (B) ...read more
Epicutaneous lugdunin recruits phagocytic cells. a Schematic overview of the mouse experiments: 6–8-week-old female C5BL/6 wild-type (WT), MyD88-ko, or 5xTLR-ko mice were epicutaneously treated with 1.5 µg lugdunin ...read more
Levels of CTGF, TGF-beta , MPO, and VEGF in silicone implant-induced capsular tissues determined by western blotting (a). A low signal was obtained for CTGF (b) and TGF-beta (c) protein in PN-KO mice (n = 6), whereas a ...read more
LCN2 mAb limited blood–brain barrier leakage and infiltration of neutrophils after tMCAo. Representative images (A) and quantification (B) of Evans blue extravasation in the ipsilateral hemispheres of mice treated ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Myeloperoxidase/MPO Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MPO
Met16-Thr718
Accession # P11247
Specificity
Detects human and mouse Myeloperoxidase/MPO in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse EPPO is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MPO
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 3 Reviews rated 4.7
using
AF3667 in the following applications:

Publications
Read Publications using
AF3667 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Myeloperoxidase/MPO Antibody [Unconjugated]

  • EC 1.11.1
  • EC 1.11.1.7
  • MPO
  • Myeloperoxidase

Background

Myeloperoxidase (MPO) is a hemeprotein that belongs to the XPO subfamily of the heme peroxidase superfamily. MPO is synthesized as a preproprotein that undergoes proteolytic processing to generate a disulfide-linked heterodimer of the N-terminal beta -subunit (12 kDa) and C-terminal alpha subunit (60 kDa). Active MPO is a tetramer of two beta -subunits and two alpha -subunits that are also disulfide-linked through the two alpha -subunits. MPO is stored in granules and is an abundant protein in neutrophils and monocytes. MPO is released upon activation to catalyze the formation of powerful oxidants such as hypochlorous acid, which kills microbes. Unprocessed pro-MPO can also be released. Mouse MPO shares 87% amino acid sequence identity with that of human MPO.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Myeloperoxidase/MPO Antibody (AF3667)(80)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Hamster, Primate, Transgenic Mouse, Xenograft.

We have publications tested in 9 applications: ELISA Capture, ELISA Development, Functional Assay, ICC, ICC/IF, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
ELISA Capture
(1)
ELISA Development
(1)
Functional Assay
(1)
ICC
(15)
ICC/IF
(1)
IHC
(60)
IHC-Fr
(1)
IHC-P
(2)
Western Blot
(10)
All Applications
Filter By Species
Human
(16)
Mouse
(62)
Rat
(3)
Hamster
(1)
Primate
(1)
Transgenic Mouse
(1)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 80. Show All 80 Publications.
Publications using AF3667 Applications Species
Wilkening, RV;Langouët-Astrié, C;Severn, MM;Federle, MJ;Horswill, AR; Identifying genetic determinants of Streptococcus pyogenes-host interactions in a murine intact skin infection model Cell reports 2023-10-26 [PMID: 37889753] (IHC, Mouse) IHC Mouse
Shi, G;Liu, L;Cao, Y;Ma, G;Zhu, Y;Xu, J;Zhang, X;Li, T;Mi, L;Jia, H;Zhang, Y;Liu, X;Zhou, Y;Li, S;Yang, G;Liu, X;Chen, F;Wang, B;Deng, Q;Zhang, S;Zhang, J; Inhibition of neutrophil extracellular trap formation ameliorates neuroinflammation and neuronal apoptosis via STING-dependent IRE1?/ASK1/JNK signaling pathway in mice with traumatic brain injury Journal of neuroinflammation 2023-09-30 [PMID: 37777772] (IHC, Mouse) IHC Mouse
Cao, L;Ma, L;Zhao, J;Wang, X;Fang, X;Li, W;Qi, Y;Tang, Y;Liu, J;Peng, S;Yang, L;Zhou, L;Li, L;Hu, X;Ji, Y;Hou, Y;Zhao, Y;Zhang, X;Zhao, YY;Zhao, Y;Wei, Y;Malik, AB;Saiyin, H;Xu, J; An unexpected role of neutrophils in clearing apoptotic hepatocytes in vivo eLife 2023-09-20 [PMID: 37728612] (IHC, Human) IHC Human
Hernandez Pichardo, A;Wilm, B;Liptrott, NJ;Murray, P; Intravenous Administration of Human Umbilical Cord Mesenchymal Stromal Cells Leads to an Inflammatory Response in the Lung Stem cells international 2023-09-05 [PMID: 37705699] (IHC, Human) IHC Human
Wu, H;Guo, C;Liu, Z;Cai, J;Wang, C;Yi, H;Sanyal, A;Puri, P;Zhou, H;Wang, XY; Neutrophils exacerbate acetaminophen-induced liver injury by producing cytotoxic interferon-? International immunopharmacology 2023-08-02 [PMID: 37541108] (IHC, Mouse) IHC Mouse
Le, PM;Pal-Ghosh, S;Menko, AS;Stepp, MA; Immune Cells Localize to Sites of Corneal Erosions in C57BL/6 Mice Biomolecules 2023-06-29 [PMID: 37509096] (IHC, Mouse) IHC Mouse
Perico, L;Morigi, M;Pezzotta, A;Locatelli, M;Imberti, B;Corna, D;Cerullo, D;Benigni, A;Remuzzi, G; SARS-CoV-2 spike protein induces lung endothelial cell dysfunction and thrombo-inflammation depending on the C3a/C3a receptor signalling Scientific reports 2023-07-14 [PMID: 37452090] (IHC, Transgenic Mouse) IHC Transgenic Mouse
Shiratori-Aso, S;Nakazawa, D;Kudo, T;Kanda, M;Ueda, Y;Watanabe-Kusunoki, K;Nishio, S;Iwasaki, S;Tsuji, T;Masuda, S;Tomaru, U;Ishizu, A;Atsumi, T; CD47 blockade ameliorates autoimmune vasculitis via efferocytosis of neutrophil extracellular traps JCI insight 2023-08-08 [PMID: 37368493] (IHC, Mouse) IHC Mouse
Huerga Encabo, H;Aramburu, IV;Garcia-Albornoz, M;Piganeau, M;Wood, H;Song, A;Ferrelli, A;Sharma, A;Minutti, CM;Domart, MC;Papazoglou, D;Gurashi, K;Llorian Sopena, M;Goldstone, R;Fallesen, T;Wang, Q;Ariza-McNaughton, L;Wiseman, DH;Batta, K;Gupta, R;Papayannopoulos, V;Bonnet, D; Loss of TET2 in human hematopoietic stem cells alters the development and function of neutrophils Cell stem cell 2023-06-01 [PMID: 37267914] (ICC, IHC, Mouse) ICC, IHC Mouse
Sung, PS;Sun, CP;Tao, MH;Hsieh, SL; Inhibition of SARS-CoV-2-mediated thromboinflammation by CLEC2.Fc EMBO molecular medicine 2023-05-22 [PMID: 37211986] (IHC, ICC, Mouse, Human) IHC, ICC Mouse, Human
Show All 80 Publications.

Reviews for Myeloperoxidase/MPO Antibody (AF3667) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Rabbit.

Reviews using AF3667:
Filter by Applications
Imaging Mass Cytometry
(1)
Immunofluorescence - paraffin
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(2)
Rabbit
(1)
All Species
Images Ratings Applications Species Date Details
Other Myeloperoxidase/MPO AF3667
Enlarge
5
reviewed by:
Aida Meghraoui
Imaging Mass Cytometry Human 10/05/2020
View

Summary

ApplicationOther
Sample TestedFFPE
SpeciesHuman
LotYBZ0319051
Other Myeloperoxidase/MPO AF3667
Enlarge
5
reviewed by:
Verified Customer
Immunofluorescence - paraffin Human 04/12/2019
View

Summary

ApplicationOther
Sample Testedhuman tonsil
SpeciesHuman
LotI don't have it any more

Comments

CommentspH9 antigen retrieval
Immunohistochemistry-Paraffin Myeloperoxidase/MPO AF3667
Enlarge
4
reviewed by:
Verified Customer
IHC-P Rabbit 01/11/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSmall intestine
SpeciesRabbit
LotYBZ0216021

Comments

CommentsWe have tested the antibody for use in rabbit.
PFA-fixed ileum embedded in paraffin was used, sections were 7 micrometer thick. Details about antigen retrieval are available upon request and outlined in the attached image.
We also performed WB analysis with these rabbit samples. Image available upon request.
Before trying with rabbit, we used samples from mouse (previously tested species) to confirm that the antibody is working in our hands. Details available upon request.
Please don't hesitte to contact us in case of questions.
We woud like to participate in the Innovator Reward. Thanks.

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FAQs for Myeloperoxidase/MPO Antibody (AF3667) (0)

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Aida Meghraoui
10/05/2020
Application: Imaging Mass Cytometry
Species: Human

Verified Customer
04/12/2019
Application: Immunofluorescence - paraffin
Species: Human

Verified Customer
01/11/2017
Application: IHC-P
Species: Rabbit

Bioinformatics

Gene Symbol MPO
Uniprot