Myeloperoxidase/MPO Antibody [Unconjugated]

Images

 
Western blot shows lysates of HL‑60 human acute promyelocytic leukemia cell line, human neutrophil cells, and mouse spleen tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Myeloperoxidase/MPO ...read more
Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for Myeloperoxidase/MPO at approximately 67 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse ...read more
Myeloperoxidase/MPO was detected in immersion fixed mouse splenocytes using Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) at 15 µg/mL for 3 hours at room ...read more
Myeloperoxidase/MPO was detected in immersion fixed paraffin-embedded sections of human spleen using 3 µg/mL Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Myeloperoxidase/MPO Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MPO
Met16-Thr718
Accession # P11247
Specificity
Detects human and mouse Myeloperoxidase/MPO in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse EPPO is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MPO
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 3 Reviews rated 4.7
using
AF3667 in the following applications:

Publications
Read Publications using
AF3667 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Myeloperoxidase/MPO Antibody [Unconjugated]

  • EC 1.11.1
  • EC 1.11.1.7
  • MPO
  • Myeloperoxidase

Background

Myeloperoxidase (MPO) is a hemeprotein that belongs to the XPO subfamily of the heme peroxidase superfamily. MPO is synthesized as a preproprotein that undergoes proteolytic processing to generate a disulfide-linked heterodimer of the N-terminal beta -subunit (12 kDa) and C-terminal alpha subunit (60 kDa). Active MPO is a tetramer of two beta -subunits and two alpha -subunits that are also disulfide-linked through the two alpha -subunits. MPO is stored in granules and is an abundant protein in neutrophils and monocytes. MPO is released upon activation to catalyze the formation of powerful oxidants such as hypochlorous acid, which kills microbes. Unprocessed pro-MPO can also be released. Mouse MPO shares 87% amino acid sequence identity with that of human MPO.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Myeloperoxidase/MPO Antibody (AF3667)(63)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Hamster, Primate, Xenograft.

We have publications tested in 8 applications: ELISA Capture, ELISA Development, Functional Assay, ICC, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
ELISA Capture
(2)
ELISA Development
(1)
Functional Assay
(1)
ICC
(11)
IHC
(34)
IHC-Fr
(2)
IHC-P
(2)
Western Blot
(10)
All Applications
Filter By Species
Human
(11)
Mouse
(47)
Rat
(2)
Hamster
(1)
Primate
(1)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 63. Show All 63 Publications.
Publications using AF3667 Applications Species
K Wang, Y Liao, X Li, R Wang, Z Zeng, M Cheng, L Gao, D Xu, F Wen, T Wang, J Chen Inhibition of neutrophil elastase prevents cigarette smoke exposure-induced formation of neutrophil extracellular traps and improves lung function in a mouse model of chronic obstructive pulmonary disease International immunopharmacology, 2022;114(0):109537. 2022 [PMID: 36495695] (ICC, Human) ICC Human
J Yang, L Jin, HS Kim, F Tian, Z Yi, K Bedi, M Ljungman, M Pasca di M, H Crawford, J Shi KDM6A Loss Recruits Tumor-Associated Neutrophils and Promotes Neutrophil Extracellular Trap Formation in Pancreatic Cancer Cancer Research, 2022;0(0):OF1-OF14. 2022 [PMID: 36306422] (ICC, Human) ICC Human
H Li, C Qiao, L Zhao, Q Jing, D Xue, Y Zhang Epigallocatechin-3-gallate reduces neutrophil extracellular trap formation and tissue injury in severe acute pancreatitis Journal of leukocyte biology, 2022;0(0):. 2022 [PMID: 35983712] (ICC, Human) ICC Human
A Gupta, K Singh, S Fatima, S Ambreen, S Zimmermann, R Younis, S Krishnan, R Rana, I Gadi, C Schwab, R Biemann, K Shahzad, V Rani, S Ali, PR Mertens, S Kohli, B Isermann Neutrophil Extracellular Traps Promote NLRP3 Inflammasome Activation and Glomerular Endothelial Dysfunction in Diabetic Kidney Disease Nutrients, 2022;14(14):. 2022 [PMID: 35889923] (IHC, Mouse) IHC Mouse
W Chen, B Li, F Jia, J Li, H Huang, C Ni, W Xia High PANX1 Expression Leads to Neutrophil Recruitment and the Formation of a High Adenosine Immunosuppressive Tumor Microenvironment in Basal-like Breast Cancer Cancers, 2022;14(14):. 2022 [PMID: 35884429] (IHC, Human) IHC Human
N Caporarell, J Lee, TX Pham, DL Jones, J Guan, PA Link, JA Meridew, G Marden, T Yamashita, CA Osborne, AV Bhagwate, SK Huang, RF Nicosia, DJ Tschumperl, M Trojanowsk, G Ligresti Dysfunctional ERG signaling drives pulmonary vascular aging and persistent fibrosis Nature Communications, 2022;13(1):4170. 2022 [PMID: 35879310] (IHC, Mouse) IHC Mouse
NPK Le, AF do Nascime, D Schneberge, CC Quach, X Zhang, GK Aulakh, W Dawicki, L Liu, JR Gordon, B Singh Deficiency of leukocyte-specific protein 1 (LSP1) alleviates asthmatic inflammation in a mouse model Respiratory Research, 2022;23(1):165. 2022 [PMID: 35733161] (IHC, Mouse) IHC Mouse
D Ye, J Yao, W Du, C Chen, Y Yang, K Yan, J Li, Y Xu, S Zang, Y Zhang, X Rong, R Zhang, A Xu, J Guo Neutrophil Extracellular Traps Mediate Acute Liver Failure in Regulation of miR-223/neutrophil Elastase Signaling in Mice Cellular and Molecular Gastroenterology and Hepatology, 2022;0(0):. 2022 [PMID: 35660025] (Western Blot, Mouse) Western Blot Mouse
C Arnholdt, K Kumaraswam, P Götz, M Kübler, M Lasch, E Deindl Depletion of gammadelta T Cells Leads to Reduced Angiogenesis and Increased Infiltration of Inflammatory M1-like Macrophages in Ischemic Muscle Tissue Cells, 2022;11(9):. 2022 [PMID: 35563796] (IHC, Mouse) IHC Mouse
H Wang, DJ Tumes, TR Hercus, KH Yip, C Aloe, R Vlahos, AF Lopez, N Wilson, CM Owczarek, S Bozinovski Blocking the human common beta subunit of the GM-CSF, IL-5 and IL-3 receptors markedly reduces hyperinflammation in ARDS models Cell Death & Disease, 2022;13(2):137. 2022 [PMID: 35145069] (IHC, Mouse) IHC Mouse
Show All 63 Publications.

Reviews for Myeloperoxidase/MPO Antibody (AF3667) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Rabbit.

Reviews using AF3667:
Filter by Applications
Imaging Mass Cytometry (IMC)
(1)
Immunofluorescence - paraffin
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(2)
Rabbit
(1)
All Species
Images Ratings Applications Species Date Details
Other Myeloperoxidase/MPO AF3667
Enlarge
5
reviewed by:
Aida Meghraoui
Imaging Mass Cytometry (IMC) Human 10/05/2020
View

Summary

ApplicationOther
Sample TestedFFPE
SpeciesHuman
LotYBZ0319051
Other Myeloperoxidase/MPO AF3667
Enlarge
5
reviewed by:
Christian Schuerch
Immunofluorescence - paraffin Human 04/12/2019
View

Summary

ApplicationOther
Sample Testedhuman tonsil
SpeciesHuman
LotI don't have it any more

Comments

CommentspH9 antigen retrieval
Immunohistochemistry-Paraffin Myeloperoxidase/MPO AF3667
Enlarge
4
reviewed by:
Andrea Puhar
IHC-P Rabbit 01/11/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSmall intestine
SpeciesRabbit
LotYBZ0216021

Comments

CommentsWe have tested the antibody for use in rabbit.
PFA-fixed ileum embedded in paraffin was used, sections were 7 micrometer thick. Details about antigen retrieval are available upon request and outlined in the attached image.
We also performed WB analysis with these rabbit samples. Image available upon request.
Before trying with rabbit, we used samples from mouse (previously tested species) to confirm that the antibody is working in our hands. Details available upon request.
Please don't hesitte to contact us in case of questions.
We woud like to participate in the Innovator Reward. Thanks.

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FAQs for Myeloperoxidase/MPO Antibody (AF3667) (0)

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Aida Meghraoui
10/05/2020
Application: Imaging Mass Cytometry (IMC)
Species: Human

Christian Schuerch
04/12/2019
Application: Immunofluorescence - paraffin
Species: Human

Andrea Puhar
01/11/2017
Application: IHC-P
Species: Rabbit

Bioinformatics

Gene Symbol MPO
Uniprot