Myeloperoxidase/MPO Antibody [Unconjugated]

Images

 
Western blot shows lysates of HL‑60 human acute promyelocytic leukemia cell line, human neutrophil cells, and mouse spleen tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Myeloperoxidase/MPO ...read more
Myeloperoxidase/MPO was detected in immersion fixed paraffin-embedded sections of human spleen using 3 µg/mL Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for Myeloperoxidase/MPO at approximately 67 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse ...read more
Myeloperoxidase/MPO was detected in immersion fixed mouse splenocytes using Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) at 15 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Myeloperoxidase/MPO Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MPO
Met16-Thr718
Accession # P11247
Specificity
Detects human and mouse Myeloperoxidase/MPO in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse EPPO is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MPO
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Simple Western 5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 1 Review rated 4
using
AF3667 in the following applications:

Publications
Read Publications using
AF3667 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Myeloperoxidase/MPO Antibody [Unconjugated]

  • EC 1.11.1
  • EC 1.11.1.7
  • Lactoperoxidase
  • MPO
  • Myeloperoxidase

Background

Myeloperoxidase (MPO) is a hemeprotein that belongs to the XPO subfamily of the heme peroxidase superfamily. MPO is synthesized as a preproprotein that undergoes proteolytic processing to generate a disulfide-linked heterodimer of the N-terminal beta -subunit (12 kDa) and C-terminal alpha subunit (60 kDa). Active MPO is a tetramer of two beta -subunits and two alpha -subunits that are also disulfide-linked through the two alpha -subunits. MPO is stored in granules and is an abundant protein in neutrophils and monocytes. MPO is released upon activation to catalyze the formation of powerful oxidants such as hypochlorous acid, which kills microbes. Unprocessed pro-MPO can also be released. Mouse MPO shares 87% amino acid sequence identity with that of human MPO.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Myeloperoxidase/MPO Antibody (AF3667)(17)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 10 applications: ELISA Development, IF, IHC, IHC Frozen, IHC Not Specified, IHC OCT-embedded, IHC-F, IHC-Fr, IHC-P, WB.


Filter By Application
ELISA Development
(1)
IF
(1)
IHC
(5)
IHC Frozen
(1)
IHC Not Specified
(1)
IHC OCT-embedded
(1)
IHC-F
(1)
IHC-Fr
(1)
IHC-P
(1)
WB
(4)
All Applications
Filter By Species
Mouse
(17)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using AF3667 Applications Species
Z Cheng, L Wang, M Qu, H Liang, W Li, Y Li, L Deng, Z Zhang, GY Yang Mesenchymal stem cells attenuate blood-brain barrier leakage after cerebral ischemia in mice J Neuroinflammation, 2018;15(1):135. 2018 [PMID: 29724240] (IHC-F,, Mouse) IHC-F Mouse
SY Cheon, JM Kim, EH Kam, CC Ho, EJ Kim, S Chung, JH Jeong, DD Lee, SW Lee, BN Koo Cell-penetrating interactomic inhibition of nuclear factor-kappa B in a mouse model of postoperative cognitive dysfunction Sci Rep, 2017;7(1):13482. 2017 [PMID: 29044209] (IHC-Fr, Mouse) IHC-Fr Mouse
MB Johnson, B Pang, DJ Gardner, S Niknam-Ben, V Soundaraja, A Bramos, DP Perrault, K Banks, GK Lee, RY Baker, GH Kim, S Lee, Y Chai, M Chen, W Li, L Kwong, YK Hong, AK Wong Topical Fibronectin Improves Wound Healing of Irradiated Skin Sci Rep, 2017;7(1):3876. 2017 [PMID: 28634413] (IHC,, Mouse) IHC Mouse
CWL Dougher, A Buffone, MJ Nemeth, M Nasirikena, EE Irons, PN Bogner, JTY Lau The blood-borne sialyltransferase ST6Gal-1 is a negative systemic regulator of granulopoiesis J. Leukoc. Biol., 2017;0(0):. 2017 [PMID: 28550122] (WB,, Mouse) WB Mouse
M Toussaint, DJ Jackson, D Swieboda, A Guedán, TD Tsouroukts, YM Ching, C Radermecke, H Makrinioti, J Aniscenko, MR Edwards, R Solari, F Farnir, V Papayannop, F Bureau, T Marichal, SL Johnston Host DNA released by NETosis promotes rhinovirus-induced type-2 allergic asthma exacerbation Nat. Med., 2017;23(6):681-691. 2017 [PMID: 28459437] (IHC,, Mouse) IHC Mouse
FM Silva, EE Oliveira, AC Gouveia, AS Brugiolo, CC Alves, JO Correa, J Gameiro, J Mattes, HC Teixeira, AP Ferreira Obesity promotes prolonged ovalbumin-induced airway inflammation modulating Th1, Th2 and Th17 immune responses in BALB/c mice Clin. Exp. Immunol, 2017;0(0):. 2017 [PMID: 28263381] (IHC,, Mouse) IHC Mouse
Luise Erpenbeck PAD4 Deficiency Decreases Inflammation and Susceptibility to Pregnancy Loss in a Mouse Model Biol. Reprod, 2016;95(6):132. 2016 [PMID: 28007693] (IHC-P,, Mouse) IHC-P Mouse
Swimming Motility Mediates the Formation of Neutrophil Extracellular Traps Induced by Flagellated Pseudomonas aeruginosa PLoS Pathog., 2016;12(11):e1005987. 2016 [PMID: 27855208] (IF,, Mouse) IF Mouse
Beneficial Effects of Sodium Phenylbutyrate Administration During Infection with Salmonella enterica serovar Typhimurium Infect Immun, 2016;0(0):. 2016 [PMID: 27382022] (WB,, Mouse) WB Mouse
Neutrophil Attack Triggers Extracellular Trap-Dependent Candida Cell Wall Remodeling and Altered Immune Recognition PLoS Pathog, 2016;12(5):e1005644. 2016 [PMID: 27223610] (IHC Not Specified,, Mouse) IHC Not Specified Mouse
Show All 17 Publications.

Review for Myeloperoxidase/MPO Antibody (AF3667) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rabbit.

Reviews using AF3667:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Rabbit
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Myeloperoxidase/MPO AF3667
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reviewed by:
Anonymous
IHC-P Rabbit 01/11/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSmall intestine
SpeciesRabbit
LotYBZ0216021

Comments

CommentsWe have tested the antibody for use in rabbit.
PFA-fixed ileum embedded in paraffin was used, sections were 7 micrometer thick. Details about antigen retrieval are available upon request and outlined in the attached image.
We also performed WB analysis with these rabbit samples. Image available upon request.
Before trying with rabbit, we used samples from mouse (previously tested species) to confirm that the antibody is working in our hands. Details available upon request.
Please don't hesitte to contact us in case of questions.
We woud like to participate in the Innovator Reward. Thanks.

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Recent Reviews

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5
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4
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1
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Anonymous
01/11/2017
Application: IHC-P
Species: Rabbit

Bioinformatics

Gene Symbol MPO
Entrez
Uniprot