Myeloperoxidase/MPO Antibody [Unconjugated]

Images

 
Western blot shows lysates of HL‑60 human acute promyelocytic leukemia cell line, human neutrophil cells, and mouse spleen tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Myeloperoxidase/MPO ...read more
Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for Myeloperoxidase/MPO at approximately 67 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse ...read more
Myeloperoxidase/MPO was detected in immersion fixed mouse splenocytes using Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) at 15 µg/mL for 3 hours at room ...read more
Myeloperoxidase/MPO was detected in immersion fixed paraffin-embedded sections of human spleen using 3 µg/mL Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Myeloperoxidase/MPO Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MPO
Met16-Thr718
Accession # P11247
Specificity
Detects human and mouse Myeloperoxidase/MPO in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse EPPO is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MPO
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 3 Reviews rated 4.7
using
AF3667 in the following applications:

Publications
Read Publications using
AF3667 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Myeloperoxidase/MPO Antibody [Unconjugated]

  • EC 1.11.1
  • EC 1.11.1.7
  • Lactoperoxidase
  • MPO
  • Myeloperoxidase

Background

Myeloperoxidase (MPO) is a hemeprotein that belongs to the XPO subfamily of the heme peroxidase superfamily. MPO is synthesized as a preproprotein that undergoes proteolytic processing to generate a disulfide-linked heterodimer of the N-terminal beta -subunit (12 kDa) and C-terminal alpha subunit (60 kDa). Active MPO is a tetramer of two beta -subunits and two alpha -subunits that are also disulfide-linked through the two alpha -subunits. MPO is stored in granules and is an abundant protein in neutrophils and monocytes. MPO is released upon activation to catalyze the formation of powerful oxidants such as hypochlorous acid, which kills microbes. Unprocessed pro-MPO can also be released. Mouse MPO shares 87% amino acid sequence identity with that of human MPO.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Myeloperoxidase/MPO Antibody (AF3667)(40)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Primate, Xenograft.

We have publications tested in 8 applications: ELISA Capture, ELISA Development, Functional Assay, ICC, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
ELISA Capture
(1)
ELISA Development
(1)
Functional Assay
(1)
ICC
(4)
IHC
(20)
IHC-Fr
(2)
IHC-P
(2)
Western Blot
(9)
All Applications
Filter By Species
Human
(5)
Mouse
(32)
Rat
(1)
Primate
(1)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 40. Show All 40 Publications.
Publications using AF3667 Applications Species
S Arandjelov, JSA Perry, M Zhou, A Ceroi, I Smirnov, SF Walk, LS Shankman, I Cambré, S Onengut-Gu, D Elewaut, TP Conrads, KS Ravichandr ELMO1 signaling is a promoter of osteoclast function and bone loss Nature Communications, 2021;12(1):4974. 2021 [PMID: 34404802] (Western Blot, Mouse) Western Blot Mouse
N Belforte, J Agostinone, L Alarcon-Ma, D Villafranc, F Dotigny, JL Cueva Varg, A Di Polo AMPK hyperactivation promotes dendrite retraction, synaptic loss, and neuronal dysfunction in glaucoma Molecular Neurodegeneration, 2021;16(1):43. 2021 [PMID: 34187514] (IHC, Mouse) IHC Mouse
R Surolia, FJ Li, Z Wang, M Kashyap, RK Srivastava, AM Traylor, P Singh, KG Dsouza, H Kim, JF Pittet, JW Zmijewski, A Agarwal, M Athar, A Ahmad, VB Antony NETosis in the pathogenesis of acute lung injury following cutaneous chemical burns JCI Insight, 2021;6(10):. 2021 [PMID: 34027893] (ICC, Human) ICC Human
M Kübler, S Beck, S Fischer, P Götz, K Kumaraswam, H Ishikawa-A, M Lasch, E Deindl Absence of Cold-Inducible RNA-Binding Protein (CIRP) Promotes Angiogenesis and Regeneration of Ischemic Tissue by Inducing M2-Like Macrophage Polarization Biomedicines, 2021;9(4):. 2021 [PMID: 33916904] (IHC, Mouse) IHC Mouse
MJ Thomas, E Major, A Benedek, I Horváth, D Máthé, R Bergmann, AM Szász, T Krenács, Z Benyó Suppression of Metastatic Melanoma Growth in Lung by Modulated Electro-Hyperthermia Monitored by a Minimally Invasive Heat Stress Testing Approach in Mice Cancers, 2020;12(12):. 2020 [PMID: 33371498] (IHC, Mouse) IHC Mouse
L Moreira-Te, PJ Stimpson, E Stavropoul, S Hadebe, P Chakravart, M Ioannou, IV Aramburu, E Herbert, SL Priestnall, A Suarez-Bon, J Sousa, KL Fonseca, Q Wang, S Vashakidze, P Rodríguez-, C Vilaplana, M Saraiva, V Papayannop, A O'Garra Type I IFN exacerbates disease in tuberculosis-susceptible mice by inducing neutrophil-mediated lung inflammation and NETosis Nat Commun, 2020;11(1):5566. 2020 [PMID: 33149141] (ICC, Mouse) ICC Mouse
F Essig, AM Kollikowsk, M Pham, L Solymosi, G Stoll, KG Haeusler, P Kraft, MK Schuhmann Immunohistological Analysis of Neutrophils and Neutrophil Extracellular Traps in Human Thrombemboli Causing Acute Ischemic Stroke Int J Mol Sci, 2020;21(19):. 2020 [PMID: 33036337] (IHC, Human) IHC Human
WJ Ouwendijk, HJ van den Ha, MW Delany, JJ van Kampen, GP van Nierop, T Mehraban, F Zaaraoui-B, WF van IJcken, JM van den Br, RD De Vries, AC Andeweg, GM Verjans Alveolar barrier disruption in varicella pneumonia is associated with neutrophil extracellular trap formation JCI Insight, 2020;0(0):. 2020 [PMID: 33021967] (IHC, Primate) IHC Primate
S Taylor, M Whitfield, J Barratt, A Didangelos The Metalloproteinase ADAMTS5 Is Expressed by Interstitial Inflammatory Cells in IgA Nephropathy and Is Proteolytically Active on the Kidney Matrix J. Immunol., 2020;0(0):. 2020 [PMID: 32917786] (Western Blot, Human) Western Blot Human
EY Lin, HJ Lai, YK Cheng, KQ Leong, LC Cheng, YC Chou, YC Peng, YH Hsu, HS Chiang Neutrophil Extracellular Traps Impair Intestinal Barrier Function during Experimental Colitis Biomedicines, 2020;8(8):. 2020 [PMID: 32764411] (ELISA Capture, Mouse) ELISA Capture Mouse
Show All 40 Publications.

Reviews for Myeloperoxidase/MPO Antibody (AF3667) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Rabbit.

Reviews using AF3667:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(2)
Rabbit
(1)
All Species
Images Ratings Applications Species Date Details
 Myeloperoxidase/MPO AF3667
Enlarge
5
reviewed by:
Verified Customer
Human 10/05/2020
View

Summary

Sample TestedFFPE
SpeciesHuman
LotYBZ0319051
 Myeloperoxidase/MPO AF3667
Enlarge
5
reviewed by:
Verified Customer
Human 04/12/2019
View

Summary

Sample Testedhuman tonsil
SpeciesHuman
LotI don't have it any more

Comments

CommentspH9 antigen retrieval
Immunohistochemistry-Paraffin Myeloperoxidase/MPO AF3667
Enlarge
4
reviewed by:
Verified Customer
IHC-P Rabbit 01/11/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSmall intestine
SpeciesRabbit
LotYBZ0216021

Comments

CommentsWe have tested the antibody for use in rabbit.
PFA-fixed ileum embedded in paraffin was used, sections were 7 micrometer thick. Details about antigen retrieval are available upon request and outlined in the attached image.
We also performed WB analysis with these rabbit samples. Image available upon request.
Before trying with rabbit, we used samples from mouse (previously tested species) to confirm that the antibody is working in our hands. Details available upon request.
Please don't hesitte to contact us in case of questions.
We woud like to participate in the Innovator Reward. Thanks.

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FAQs for Myeloperoxidase/MPO Antibody (AF3667) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF3667

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Verified Customer
10/05/2020
Application:
Species: Human

Verified Customer
04/12/2019
Application:
Species: Human

Verified Customer
01/11/2017
Application: IHC-P
Species: Rabbit

Bioinformatics

Gene Symbol MPO
Uniprot