iNOS Overexpression Lysate (Native)


Western Blot: iNOS Overexpression Lysate (Adult Normal) [NBL1-13721] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for iNOS.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

iNOS Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC211819 and based on accession number NM_000625.
C-terminal DDK tag
Homo sapiens nitric oxide synthase 2, inducible (NOS2), mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
130.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for iNOS

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for iNOS Overexpression Lysate (Native)

  • EC
  • Hepatocyte NOS
  • HEP-NOSPeptidyl-cysteine S-nitrosylase NOS2
  • Inducible NO synthase
  • Inducible NOS
  • INOS
  • nitric oxide synthase 2, inducible
  • nitric oxide synthase 2A (inducible, hepatocytes)
  • nitric oxide synthase, inducible
  • nitric oxide synthase, macrophage
  • NOS
  • NOS, type II
  • NOS2
  • NOS2A
  • NOS2ANOS type II


Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq]. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for iNOS Lysate (NBL1-13721) (0)

There are no publications for iNOS Lysate (NBL1-13721).
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for iNOS Lysate (NBL1-13721). (Showing 1 - 1 of 1 FAQ).

  1. We need to evaluate the level of iNOS in Rat sciatic nerve by westernblot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
    • We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.

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Research Areas for iNOS Lysate (NBL1-13721)

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Blogs on iNOS.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol NOS2

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