Recombinant Human IL-1 beta/IL-1F2 Protein

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1 μg/lane of Recombinant Human IL-1 beta /IL-1F2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is <12 pg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human IL-1 beta/IL-1F2 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is <12 pg/mL.
The specific activity of recombinant human IL-1 beta is approximately 1.4 x 105 IU/μg, which is calibrated against recombinant human IL-1 beta WHO International Standard (NIBSC code: 86/680).
Source
E. coli-derived human IL-1 beta/IL-1F2 protein
Ala117-Ser269
Accession #
N-terminal Sequence
Ala117 & Pro118
Protein/Peptide Type
Recombinant Proteins
Gene
IL1B
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17 kDa, reducing conditions
Publications
Read Publications using
201-LB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 50-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is covered by U.S. patent: 5,681,933.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-1 beta/IL-1F2 Protein

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.

  1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
  4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
  5. March, C.J. et al. (1985) Nature 315:641.
  6. Auron, P.E. et al. (1984) Proc. Natl. Acad. Sci. 81:7907.
  7. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

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Publications for IL-1 beta/IL-1F2 (201-LB)(362)

We have publications tested in 13 confirmed species: Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Hamster, N/A, Porcine, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca mulatta (Rhesus Macaque), Primate - Pan troglodytes (Chimpanzee), Rabbit.

We have publications tested in 8 applications: Binding Assay, Bioassay, Bioassay Standard, Cell Culture Treatment, ELISA Standard, In Vivo, Standard, cell culture.


Filter By Application
Binding Assay
(1)
Bioassay
(324)
Bioassay Standard
(1)
Cell Culture Treatment
(1)
ELISA Standard
(11)
In Vivo
(8)
Standard
(1)
cell culture
(3)
All Applications
Filter By Species
Human
(303)
Mouse
(14)
Rat
(6)
Bovine
(3)
Canine
(3)
Guinea Pig
(1)
Hamster
(1)
N/A
(12)
Porcine
(2)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(5)
Primate - Pan troglodytes (Chimpanzee)
(1)
Rabbit
(4)
All Species
Showing Publications 1 - 10 of 362. Show All 362 Publications.
Publications using 201-LB Applications Species
T Hochdörfer, C Winkler, K Pardali, J Mjösberg Expression of c-Kit discriminates between two functionally distinct subsets of human type 2 innate lymphoid cells Eur. J. Immunol., 2019;0(0):. 2019 [PMID: 30892687] (Bioassay, Human) Bioassay Human
P Siengdee, W Pradit, S Chomdej, K Nganvongpa Determination of two fluoroquinolones and their combinations with hyaluronan effect in in vitro canine cartilage explants PeerJ, 2019;7(0):e6553. 2019 [PMID: 30881764] (Bioassay, Canine) Bioassay Canine
A Castellano, I Santin, A Olazagoiti, I Romero-Gar, A Jauregi-Mi, M Legarda, JR Bilbao A novel RT-QPCR-based assay for the relative quantification of residue specific m6A RNA methylation Sci Rep, 2019;9(1):4220. 2019 [PMID: 30862814] (Bioassay, Human) Bioassay Human
KC Doh, BM Kim, KW Kim, BH Chung, CW Yang Effects of resveratrol on Th17 cell-related immune responses under tacrolimus-based immunosuppression BMC Complement Altern Med, 2019;19(1):54. 2019 [PMID: 30832648] (Bioassay, Human) Bioassay Human
A Kizilyer, MV Singh, VB Singh, S Suwunnakor, J Palis, SB Maggirwar Inhibition of Tropomyosin Receptor Kinase A Signaling Negatively Regulates Megakaryopoiesis and induces Thrombopoiesis Sci Rep, 2019;9(1):2781. 2019 [PMID: 30808933] (Bioassay, Human) Bioassay Human
K Spekker-Bo, CM Ufermann, M Maywald, A Zimmermann, A Domröse, C Woite, W Däubener, SK Eller hCMV-Mediated Immune Escape Mechanisms Favor Pathogen Growth and Disturb the Immune Privilege of the Eye Int J Mol Sci, 2019;20(4):. 2019 [PMID: 30781494] (Bioassay, Human) Bioassay Human
HG Lee, JU Lee, DH Kim, S Lim, I Kang, JM Choi Pathogenic function of bystander-activated memory-like CD4+ T cells in autoimmune encephalomyelitis Nat Commun, 2019;10(1):709. 2019 [PMID: 30755603] (Bioassay, Human) Bioassay Human
S Botto, J Abraham, N Mizuno, K Pryke, B Gall, I Landais, DN Streblow, KJ Fruh, VR DeFilippis Human Cytomegalovirus Immediate Early 86-kDa Protein Blocks Transcription and Induces Degradation of the Immature Interleukin-1? Protein during Virion-Mediated Activation of the AIM2 Inflammasome MBio, 2019;10(1):. 2019 [PMID: 30755509] (Bioassay, Human) Bioassay Human
K Kuranda, S Caillat-Zu, S You, R Mallone In�Vitro Expansion of Anti-viral T Cells from Cord Blood by Accelerated Co-cultured Dendritic Cells Mol Ther Methods Clin Dev, 2019;13(0):112-120. 2019 [PMID: 30740473] (Bioassay, Human) Bioassay Human
BA Kane, H An, P Rajasekari, HP McNeil, K Bryant, N Tedla Differential expression and regulation of the non-integrin 37/67-kDa laminin receptor on peripheral blood leukocytes of healthy individuals and patients with rheumatoid arthritis Sci Rep, 2019;9(1):1149. 2019 [PMID: 30718719] (Bioassay, Human) Bioassay Human
Show All 362 Publications.

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Bioinformatics

Gene Symbol IL1B
Uniprot