Recombinant Human IL-1 beta/IL-1F2 Protein

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1 μg/lane of Recombinant Human IL-1 beta /IL-1F2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is <12 pg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human IL-1 beta/IL-1F2 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is <12 pg/mL.
The specific activity of recombinant human IL-1 beta is approximately 1.4 x 105 IU/μg, which is calibrated against recombinant human IL-1 beta WHO International Standard (NIBSC code: 86/680).
Source
E. coli-derived human IL-1 beta/IL-1F2 protein
Ala117-Ser269
Accession #
N-terminal Sequence
Ala117 & Pro118
Protein/Peptide Type
Recombinant Proteins
Gene
IL1B
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17 kDa, reducing conditions
Publications
Read Publications using
201-LB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is covered by U.S. patent: 5,681,933.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-1 beta/IL-1F2 Protein

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.

  1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
  4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
  5. March, C.J. et al. (1985) Nature 315:641.
  6. Auron, P.E. et al. (1984) Proc. Natl. Acad. Sci. 81:7907.
  7. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

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Publications for IL-1 beta/IL-1F2 (201-LB)(334)

We have publications tested in 13 confirmed species: Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Hamster, N/A, Porcine, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca mulatta (Rhesus Macaque), Primate - Pan troglodytes (Chimpanzee), Rabbit.

We have publications tested in 7 applications: Binding Assay, Bioassay, Bioassay Standard, ELISA Standard, In Vivo, Standard, cell culture.


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Binding Assay
(1)
Bioassay
(301)
Bioassay Standard
(1)
ELISA Standard
(11)
In Vivo
(8)
Standard
(1)
cell culture
(3)
All Applications
Filter By Species
Human
(277)
Mouse
(14)
Rat
(6)
Bovine
(3)
Canine
(2)
Guinea Pig
(1)
Hamster
(1)
N/A
(12)
Porcine
(2)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(5)
Primate - Pan troglodytes (Chimpanzee)
(1)
Rabbit
(4)
All Species
Showing Publications 1 - 10 of 334. Show All 334 Publications.
Publications using 201-LB Applications Species
M Schwede, EM Wilfong, RL Zemans, PJ Lee, C Dos Santos, X Fang, MA Matthay Effects of bone marrow-derived mesenchymal stromal cells on gene expression in human alveolar type II cells exposed to TNF-?, IL-1?, and IFN-? Physiol Rep, 2018;6(16):e13831. 2018 [PMID: 30136410] (Bioassay, Human) Bioassay Human
KA Cavassani, RJ Meza, DM Habiel, JF Chen, A Montes, M Tripathi, GA Martins, TR Crother, S You, CM Hogaboam, N Bhowmick, EM Posadas Circulating monocytes from prostate cancer patients promote invasion and motility of epithelial cells Cancer Med, 2018;0(0):. 2018 [PMID: 30094958] (Human) Human
SL Figenschau, E Knutsen, I Urbarova, C Fenton, B Elston, M Perander, ES Mortensen, KA Fenton ICAM1 expression is induced by proinflammatory cytokines and associated with TLS formation in aggressive breast cancer subtypes Sci Rep, 2018;8(1):11720. 2018 [PMID: 30082828] (Bioassay, Human) Bioassay Human
C Angiolilli, PA Kabala, AM Grabiec, M Rossato, WS Lai, G Fossati, P Mascagni, C Steinkühle, PJ Blackshear, KA Reedquist, DL Baeten, TRDJ Radstake Control of cytokine mRNA degradation by the histone deacetylase inhibitor ITF2357 in rheumatoid arthritis fibroblast-like synoviocytes: beyond transcriptional regulation Arthritis Res. Ther., 2018;20(1):148. 2018 [PMID: 30029685] (Bioassay, Human) Bioassay Human
JK Brenke, GM Popowicz, K Schorpp, I Rothenaign, M Roesner, I Meininger, C Kalinski, L Ringelstet, O R'kyek, G Jürjens, M Vincendeau, O Plettenbur, M Sattler, D Krappmann, K Hadian Targeting TRAF6 E3 ligase activity with a small molecule inhibitor combats autoimmunity J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29950522] (Bioassay, Human) Bioassay Human
JA Marwick, R Mills, O Kay, K Michail, J Stephen, AG Rossi, I Dransfield, N Hirani Neutrophils induce macrophage anti-inflammatory reprogramming by suppressing NF-?B activation Cell Death Dis, 2018;9(6):665. 2018 [PMID: 29867198] (Bioassay, Human) Bioassay Human
A Kock, K Larsson, F Bergqvist, N Eissler, LHM Elfman, J Raouf, M Korotkova, JI Johnsen, PJ Jakobsson, P Kogner Inhibition of Microsomal Prostaglandin E Synthase-1 in Cancer-Associated Fibroblasts Suppresses Neuroblastoma Tumor Growth EBioMedicine, 2018;32(0):84-92. 2018 [PMID: 29804818] (Bioassay, Human) Bioassay Human
E Vorkapic, A Kunath, D Wågsäter Effects of osteoprotegerin/TNFRSF11B in two models of abdominal aortic aneurysms Mol Med Rep, 2018;0(0):. 2018 [PMID: 29749489] (Bioassay, Human) Bioassay Human
SJ Mancini, D Boyd, OJ Katwan, A Strembitsk, TA Almabrouk, S Kennedy, TM Palmer, IP Salt Canagliflozin inhibits interleukin-1?-stimulated cytokine and chemokine secretion in vascular endothelial cells by AMP-activated protein kinase-dependent and -independent mechanisms Sci Rep, 2018;8(1):5276. 2018 [PMID: 29588466] (Bioassay, Human) Bioassay Human
O Sokolova, T Kähne, K Bryan, M Naumann Interactome analysis of transforming growth factor-?-activated kinase 1 inHelicobacter pylori-infected cells revealed novel regulators tripartite motif 28 and CDC37 Oncotarget, 2018;9(18):14366-14381. 2018 [PMID: 29581850] (Bioassay, Human) Bioassay Human
Show All 334 Publications.

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IL-1 was originally identified and cloned as a lymphocyte mitogen and much later, was found to be comprised of two closely related but distinct proteins, interleukin 1 alpha (IL-1 alpha) and interleukin 1 beta (IL-1 beta). Both these proteins bind...  Read full blog post.

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Bioinformatics

Gene Symbol IL1B
Entrez
Uniprot