Recombinant Human IL-1 beta/IL-1F2 Protein

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Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is <12 pg/mL.
1 μg/lane of Recombinant Human IL-1 beta /IL-1F2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human IL-1 beta/IL-1F2 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is <12 pg/mL.
Source
E. coli-derived human IL-1 beta/IL-1F2 protein
Ala117-Ser269
Accession #
N-terminal Sequence
Ala117 & Pro118
Protein/Peptide Type
Recombinant Proteins
Gene
IL1B
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17 kDa, reducing conditions
Publications
Read Publications using
201-LB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
*The 1 mg pack size (01M) can be stored for 1 month at 2 to 8 °C under sterile conditions after opening.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 50-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is covered by U.S. patent: 5,681,933.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-1 beta/IL-1F2 Protein

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta .

  1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
  4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
  5. March, C.J. et al. (1985) Nature 315:641.
  6. Auron, P.E. et al. (1984) Proc. Natl. Acad. Sci. 81:7907.
  7. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

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201-LB
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Publications for IL-1 beta/IL-1F2 (201-LB)(563)

We have publications tested in 13 confirmed species: Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Hamster, Porcine, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca mulatta (Rhesus Macaque), Primate - Pan troglodytes (Chimpanzee), Rabbit, Transgenic Mouse.

We have publications tested in 11 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Standard), ELISA Capture, In Vivo, Inhibition, Stimulation, Surface Plasmon Resonance, Western Blot, Western Blot Control.


Filter By Application
Binding Assay
(2)
Bioassay
(479)
Cell Culture
(50)
ELISA (Standard)
(12)
ELISA Capture
(3)
In Vivo
(9)
Inhibition
(1)
Stimulation
(2)
Surface Plasmon Resonance
(1)
Western Blot
(1)
Western Blot Control
(1)
All Applications
Filter By Species
Human
(492)
Mouse
(29)
Rat
(15)
Bovine
(5)
Canine
(3)
Guinea Pig
(1)
Hamster
(1)
Porcine
(3)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(5)
Primate - Pan troglodytes (Chimpanzee)
(1)
Rabbit
(4)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 563. Show All 563 Publications.
Publications using 201-LB Applications Species
Ma, X;Cao, L;Raneri, M;Wang, H;Cao, Q;Zhao, Y;Bediaga, NG;Naselli, G;Harrison, LC;Hawthorne, WJ;Hu, M;Yi, S;O'Connell, PJ; Human HLA-DR+CD27+ regulatory T cells show enhanced antigen-specific suppressive function JCI insight 2023-10-24 [PMID: 37874660] (Bioassay, Human) Bioassay Human
Yang, YS;Lin, C;Ma, H;Xie, J;Kaplan, FS;Gao, G;Shim, JH; AAV-Mediated Targeting of the Activin A-ACVR1R206H Signaling in Fibrodysplasia Ossificans Progressiva Biomolecules 2023-09-08 [PMID: 37759764] (Bioassay, Human, Transgenic Mouse) Bioassay Human, Transgenic Mouse
Ruan, Q;Wang, C;Zhang, Y;Sun, J; Brevilin A attenuates cartilage destruction in osteoarthritis mouse model by inhibiting inflammation and ferroptosis via SIRT1/Nrf2/GPX4 signaling pathway International immunopharmacology 2023-09-15 [PMID: 37717314] (Bioassay, Mouse) Bioassay Mouse
Twarog, M;Schustak, J;Xu, Y;Coble, M;Dolan, K;Esterberg, R;Huang, Q;Saint-Geniez, M;Bao, Y; TNF? induced by DNA-sensing in macrophage compromises retinal pigment epithelial (RPE) barrier function Scientific reports 2023-09-02 [PMID: 37660150] (Bioassay, Human) Bioassay Human
Vaikunthanathan, T;Landmann, E;Correa, DM;Romano, M;Trevelin, SC;Peng, Q;Crespo, E;Corrado, M;Lozano, JJ;Pearce, EL;Perpinan, E;Zoccarato, A;Siew, L;Edwards-Hicks, J;Khan, R;Luu, NT;Thursz, MR;Newsome, PN;Martinez-Llordella, M;Shah, N;Lechler, RI;Shah, AM;Sanchez-Fueyo, A;Lombardi, G;Safinia, N; Dysregulated anti-oxidant signalling and compromised mitochondrial integrity negatively influence regulatory T cell function and viability in liver disease EBioMedicine 2023-08-30 [PMID: 37657135] (Bioassay, Human) Bioassay Human
Zhu, Y;Neelamegham, S; Knockout studies using CD34+ hematopoietic cells suggest that CD44 is a physiological human neutrophil E-selectin ligand bioRxiv : the preprint server for biology 2023-08-19 [PMID: 37645985] (Bioassay, Human) Bioassay Human
Ciampa, EJ;Flahardy, P;Srinivasan, H;Jacobs, C;Tsai, L;Karumanchi, SA;Parikh, SM; Hypoxia-inducible factor 1 signaling drives placental aging and can provoke preterm labor eLife 2023-08-23 [PMID: 37610425] (Bioassay, Human) Bioassay Human
Kuppa, SS;Kim, HK;Kang, JY;Lee, SC;Yang, HY;Sankaranarayanan, J;Seon, JK; Polynucleotides Suppress Inflammation and Stimulate Matrix Synthesis in an In Vitro Cell-Based Osteoarthritis Model International journal of molecular sciences 2023-07-31 [PMID: 37569659] (Bioassay, Human) Bioassay Human
Sonmez Kaplan, S;Sazak Ovecoglu, H;Genc, D;Akkoc, T; TNF-?, IL-1B and IL-6 affect the differentiation ability of dental pulp stem cells BMC oral health 2023-08-11 [PMID: 37568110] (Bioassay, Human) Bioassay Human
Shimizu, K;Kikuta, J;Ohta, Y;Uchida, Y;Miyamoto, Y;Morimoto, A;Yari, S;Sato, T;Kamakura, T;Oshima, K;Imai, R;Liu, YC;Okuzaki, D;Hara, T;Motooka, D;Emoto, N;Inohara, H;Ishii, M; Single-cell transcriptomics of human cholesteatoma identifies an activin A-producing osteoclastogenic fibroblast subset inducing bone destruction Nature communications 2023-08-03 [PMID: 37537159] (Cell Culture, Mouse) Cell Culture Mouse
Show All 563 Publications.

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The NLRP3 Inflammasome: Macrophage Activator & Pathology Driver
By Victoria Osinski, PhDWhat is the NLRP3 Inflammasome?With its critical role in innate immunity, the nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome ...  Read full blog post.

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By Hunter MartinezWhat is Immunometabolism?It is well established that abnormal metabolic environments can be a risk factor for disease development. One characteristic example is the role of dyslipidemia (high lev...  Read full blog post.

Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

IL-1 beta (interleukin 1 beta, lymphocyte activating factor (LAF))
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Bioinformatics

Gene Symbol IL1B
Uniprot