Recombinant Human IL-1 beta/IL-1F2 Protein

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1 μg/lane of Recombinant Human IL-1 beta /IL-1F2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is <12 pg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human IL-1 beta/IL-1F2 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is <12 pg/mL.
Source
E. coli-derived human IL-1 beta/IL-1F2 protein
Ala117-Ser269
Accession #
N-terminal Sequence
Ala117 & Pro118
Protein/Peptide Type
Recombinant Proteins
Gene
IL1B
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17 kDa, reducing conditions
Publications
Read Publications using
201-LB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
*The 1 mg pack size (01M) can be stored for 1 month at 2 to 8 °C under sterile conditions after opening.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 50-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is covered by U.S. patent: 5,681,933.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-1 beta/IL-1F2 Protein

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta .

  1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
  4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
  5. March, C.J. et al. (1985) Nature 315:641.
  6. Auron, P.E. et al. (1984) Proc. Natl. Acad. Sci. 81:7907.
  7. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

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201-LB
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Publications for IL-1 beta/IL-1F2 (201-LB)(515)

We have publications tested in 13 confirmed species: Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Hamster, N/A, Porcine, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca mulatta (Rhesus Macaque), Primate - Pan troglodytes (Chimpanzee), Rabbit.

We have publications tested in 10 applications: Activation, Binding Assay, Bioassay, Cell Culture, ELISA (Standard), ELISA Capture, In Vivo, Standard, Stimulation, Western Blot Control.


Filter By Application
Activation
(1)
Binding Assay
(1)
Bioassay
(432)
Cell Culture
(41)
ELISA (Standard)
(10)
ELISA Capture
(1)
In Vivo
(8)
Standard
(1)
Stimulation
(2)
Western Blot Control
(1)
All Applications
Filter By Species
Human
(444)
Mouse
(19)
Rat
(11)
Bovine
(4)
Canine
(3)
Guinea Pig
(1)
Hamster
(1)
N/A
(11)
Porcine
(3)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(5)
Primate - Pan troglodytes (Chimpanzee)
(1)
Rabbit
(4)
All Species
Showing Publications 1 - 10 of 515. Show All 515 Publications.
Publications using 201-LB Applications Species
Y Mu, L Wang, L Fu, Q Li Knockdown of LMX1B Suppressed Cell Apoptosis and Inflammatory Response in IL-1beta-Induced Human Osteoarthritis Chondrocytes through NF-kappaB and NLRP3 Signal Pathway Mediators of Inflammation, 2022;2022(0):1870579. 2022 [PMID: 36133743] (Bioassay, Human) Bioassay Human
R Chandrasek, C Mathieu, R Sheth, AP Cheng, D Fong, R McCormack, H El-Gabalaw, S Alishetty, M Paige, CD Hoemann UDP-glucose dehydrogenase (UGDH) activity is suppressed by peroxide and promoted by PDGF in fibroblast-like synoviocytes: Evidence of a redox control mechanism PLoS ONE, 2022;17(9):e0274420. 2022 [PMID: 36107941] (Bioassay, Human) Bioassay Human
Y Yao, R Li, D Liu, L Long, N He Rosmarinic acid alleviates acetaminophen-induced hepatotoxicity by targeting Nrf2 and NEK7-NLRP3 signaling pathway Ecotoxicology and environmental safety, 2022;241(0):113773. 2022 [PMID: 35753269]
MY Wang, CM Zhang, HH Zhou, ZB Ge, CC Su, ZH Lou, XY Zhang, TT Xu, SY Li, L Zhu, YL Zhou, Y Wu, SR Ji Identification of a distal enhancer that determines the expression pattern of acute phase marker C-reactive protein Oncogene, 2022;0(0):102160. 2022 [PMID: 35724961] (Bioassay, Human) Bioassay Human
T Ungsudecha, S Honsawek, J Jittikoon, W Udomsinpra Clusterin exacerbates interleukin-1beta-induced inflammation via suppressing PI3K/Akt pathway in human fibroblast-like synoviocytes of knee osteoarthritis Oncogene, 2022;12(1):9963. 2022 [PMID: 35705674] (Bioassay, Human) Bioassay Human
W Hsu, YT Shih, MS Lee, HY Huang, WN Wu Bead Number Effect in a Magnetic-Beads-Based Digital Microfluidic Immunoassay Biosensors, 2022;12(5):. 2022 [PMID: 35624641] (ELISA Capture, Human) ELISA Capture Human
R Boi, K Ebefors, M Henricsson, J Borén, J Nyström Modified lipid metabolism and cytosolic phospholipase A2 activation in mesangial cells under pro-inflammatory conditions Scientific Reports, 2022;12(1):7322. 2022 [PMID: 35513427] (Cell Culture, Human) Cell Culture Human
A Shetty, SK Tripathi, S Junttila, T Buchacher, R Biradar, SD Bhosale, T Envall, A Laiho, R Moulder, O Rasool, S Galande, LL Elo, R Lahesmaa A systematic comparison of FOSL1, FOSL2 and BATF-mediated transcriptional regulation during early human Th17 differentiation Nucleic Acids Research, 2022;50(9):4938-4958. 2022 [PMID: 35511484] (Bioassay, Human) Bioassay Human
Y Huang, E Motta, C Nanvuma, LD Kuhrt, Y Yuan, P Xia, M Lubas, S Zhu, M Schnauss, N Qazi, F Hu, H Zhang, T Lei, M Synowitz, C Flüh, H Kettenmann Microglia/macrophage-derived human CCL18 promotes glioma progression via CCR8-ACP5 axis analyzed in humanized slice model Cell Reports, 2022;39(2):110670. 2022 [PMID: 35417708] (Bioassay, Human) Bioassay Human
R Giri, EC Hoedt, S Khushi, AA Salim, AS Bergot, V Schreiber, R Thomas, MA McGuckin, TH Florin, M Morrison, RJ Capon, P Ó Cuív, J Begun Secreted NF-kappaB suppressive microbial metabolites modulate gut inflammation Cell Reports, 2022;39(2):110646. 2022 [PMID: 35417687] (Bioassay, Human) Bioassay Human
Show All 515 Publications.

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Bioinformatics

Gene Symbol IL1B
Uniprot