nNOS Antibody

Images

 
Western Blot: nNOS Antibody [NB100-858] - Staining of human skeletal muscle lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: nNOS Antibody [NB100-858] - Staining of paraffin embedded human colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, GP, MkSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA, IHC-WhMt
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

nNOS Antibody Summary

Immunogen
Synthetic peptide with sequence ESKKDTDEVFSS, representing the C-terminus of the human protein (residues 1423-1434) according to NP_000611.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NOS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3-1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA detection limit 1:32000
  • Immunohistochemistry Whole-Mount
Application Notes
Immunofluorescence: This product has been successfully used in ICC/IF as reported in the literature (PMID: 20140458). It has also been used succesfully in IHC (PMID: 20140458). IHC:Use in Immunohistochemistry-Frozen and Immunohistochemistry whole mount reported in scientific literature (PMID 24728885). Western blot: Approx 160-170kDa band observed in human skeletal muscle and mouse brain lysates (calculated MW of 161kDa according to NP_000611.1). An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide.
Theoretical MW
161 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Skeletal Muscle Lysate (NB820-59253)
nNOS Lysate (NBL1-13719)
Reviewed Applications
Read 1 Review rated 4
using
NB100-858 in the following applications:

Publications
Read Publications using
NB100-858 in the following applications:

Reactivity Notes

Expected from sequence similarity: Dog, Cow. Guinea Pig reactivity reported in scientific literature (PMID: 26397368). Mouse reactivity reported in scientific literature (PMID: 26073142). IHC Successful results were reported by customers in Rat Cerebral Cortex and in Marmoset Cerebellum.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for nNOS Antibody

  • bNOS
  • brain
  • Constitutive NOS
  • EC 1.14.13.39
  • IHPS1
  • Neuronal NOS
  • nitric oxide synthase 1 (neuronal)
  • nNOS
  • N-NOS
  • nNOSNOS type I
  • NOS
  • NOS1
  • Peptidyl-cysteine S-nitrosylase NOS1

Background

Three isoforms of nitric oxide synthase (NOS) have been identified. All are homodimers with subunits of 130-160 kDa. All have binding sites for NADPH, FAD, and FMN near the carboxyl terminus (the reductase domain), and binding sites for tetrahydrobiopterin (BH4) and heme near the amino terminus (the oxygenase domain). The reductase and oxygenase domains are linked by a calmodulin (CaM) binding site. Occupation of this site facilitates electron transfer from the cofactors in the reductase domain to heme during nitric oxide production. NOS catalyzes the conversion of arginine to citrulline and nitric oxide (NO). Neuronal nitric oxide synthase (nNOS, bNOS, cNOS, Type I) is associated with the post-synaptic density protein (PSD-95) in the neuronal membrane. In response to increased intracellular Ca2+, nNOS interacts with CaM. The Ca2+-CaM complex, in combination with BH4, binds to nNOS and induces its translocation from the plasma membrane to the cytoplasm. The dephosphorylation of nNOS by calcineurin initiates the production NO. NO activates guanylyl cyclase (GC) and activates the various cGMP-regulated signaling pathways. nNOS is in activated by phosphorylation by protein kinase A (PKA) or protein kinase C (PKC).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for nNOS Antibody (NB100-858)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Guinea Pig.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-Fr, IHC-WhMt.


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ICC/IF
(1)
IHC
(1)
IHC-Fr
(3)
IHC-WhMt
(2)
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Human
(1)
Mouse
(4)
Guinea Pig
(1)
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Showing Publications 1 - 8 of 8.
Publications using NB100-858 Applications Species
Carbone SE, Jovanovska V, Brookes SJ, Nurgali K et al. Electrophysiological and morphological changes in colonic myenteric neurons from chemotherapy-treated patients: a pilot study Neurogastroenterol. Motil. Feb 22 2016 [PMID: 26909894] (IHC-WhMt, Human) IHC-WhMt Human
Robinson AM, Miller S, Payne N et al. Neuroprotective Potential of Mesenchymal Stem Cell-Based Therapy in Acute Stages of TNBS-Induced Colitis in Guinea-Pigs. PLoS ONE 2015 Sep 24 [PMID: 26397368] (IHC-Fr, Guinea Pig) IHC-Fr Guinea Pig
Petrie CN, Armitage MN, Kawaja MD. Myenteric expression of nerve growth factor and the p75 neurotrophin receptor regulate axonal remodeling as a consequence of colonic inflammation in mice Exp. Neurol. 2015 Jun 11 [PMID: 26073142] (IHC-Fr, Mouse) IHC-Fr Mouse
Pelayo JC, Veldhuis NA, Eriksson EM et al. Localisation and activation of the neurokinin 1 receptor in the enteric nervous system of the mouse distal colon. Cell Tissue Res. 2014 Apr 12 [PMID: 24728885] (IHC-Fr, IHC-WhMt, Mouse) IHC-Fr, IHC-WhMt Mouse
Zhao P, Canals M, Murphy JE et al. Agonist-biased Trafficking of Somatostatin Receptor 2A in Enteric Neurons. J Biol Chem. 2013 Sep 6 [PMID: 23913690] (IHC, Mouse) IHC Mouse
Wafai L, Taher M, Jovanovska V et al. Effects of oxaliplatin on mouse myenteric neurons and colonic motility Front Neurosci 2013 [PMID: 23486839] (ICC/IF, Mouse) ICC/IF Mouse
Poole DP, Godfrey C, Cattaruzza F et al. Expression and function of the bile acid receptor GpBAR1 (TGR5) in the murine enteric nervous system. Neurogastroenterol Motil. 2010 Jul. [PMID: 20236244]
Fried HU, Kaupp UB, Muller F. Hyperpolarization-activated and cyclic nucleotide-gated channels are differentially expressed in juxtaglomerular cells in the olfactory bulb of mice. Cell Tissue Res 2010 Mar [PMID: 20140458]

Review for nNOS Antibody (NB100-858) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-858:
Filter by Applications
ICC
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ICC Mouse 12/29/2014
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 23486839
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23486839

Details

Detection NotesSee PMID 23486839

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 23486839.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for nNOS Antibody (NB100-858) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

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Recent Reviews

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Anonymous
12/29/2014
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol NOS1
Entrez
Uniprot