COX-2 Antibody

Images

 
Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689] - Formalin fixed paraffin embedded colon carcinoma stained with COX-2 antibody.
Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689] - Formalin fixed paraffin embedded human colon carcinoma stained with COX2 antibody.
Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689] - Formalin fixed paraffin embedded human colon carcinoma stained with COX2 antibody.
Simple Western: COX-2 Antibody [NB100-689] - Simple Western lane view shows a specific band for COX2 in 1.0 mg/ml of Rat Brain at an antibody concentration of 1:100. This experiment was performed under reducing ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

COX-2 Antibody Summary

Immunogen
Recombinant protein corresponding to the C-terminus of rat COX2 (the exact amino acid sequence is considered to be proprietary information).
Localization
Microsomal membrane, cytoplasmic
Specificity
This reacts with the 70 kDa protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PTGS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Simple Western 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:100
Application Notes
Immunohistochemistry (paraffin-embedded sections): Use at a dilution of 1/50-1/100* for 10 min at RT. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Control
Lung Lysate (NB820-59432)
Colon Lysate (NB820-59425)
COX-2 Lysate (NBP2-66242)
Reviewed Applications
Read 1 Review rated 2
using
NB100-689 in the following applications:

Publications
Read Publications using
NB100-689 in the following applications:

  • IHC
    1 publication
  • 1 publication
  • WB
    8 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS with 2% BSA
Preservative
10mM Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for COX-2 Antibody

  • COX2
  • COX-2
  • COX2cyclooxygenase 2b
  • cyclooxygenase-2
  • EC 1.14.99
  • EC 1.14.99.1
  • GRIPGHS
  • hCox-2
  • PGG/HS
  • PGH synthase 2
  • PGHS-2
  • PHS II
  • PHS-2
  • PHS-II
  • prostaglandin G/H synthase 2
  • prostaglandin G/H synthase and cyclooxygenase
  • Prostaglandin H2 synthase 2
  • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase andcyclooxygenase)
  • Prostaglandin-endoperoxide synthase 2
  • PTGS2

Background

COX2 (Cyclooxygenase 2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initally identified as immediate early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P

Publications for COX-2 Antibody (NB100-689)(16)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(1)
IHC-P
(1)
WB
(8)
All Applications
Filter By Species
Human
(4)
Mouse
(7)
Rat
(1)
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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB100-689 Applications Species
Boisvert NC, Holterman CE, Thibodeau JF et al. Hyperfiltration in Ubiquitin C-Terminal Hydrolase L1 -Deleted Mice Clin. Sci. May 8 2018 [PMID: 29739827] (Mouse) Mouse
Jung Yu Yeon, Son Dong Ju, Lee Hye Lim et al. Loss of Parkin reduces inflammatory arthritis by inhibiting p53 degradation. Redox Biology 2017 [PMID: 28395174] (WB, Mouse) WB Mouse
Grimmig T, Matthes N, Hoeland K et al. TLR7 and TLR8 expression increases tumor cell proliferation and promotes chemoresistance in human pancreatic cancer Int. J. Oncol. 2015 Jul 02 [PMID: 26134824] (WB, Human)

Details:
COX-2 antibody was used for WB analysis of lysates from human normal pancreatic tissues as well as pancreatic cancer tissue samples (Figure 4).
WB Human
Torricelli F, Mandato VD, Farnetti E et al. Polymorphisms in cyclooxygenase-2 gene in endometrial cancer patients Tumour Biol. 2015 Apr 22 [PMID: 25900875]
Abdel-Daim MM, Farouk SM, Madkour FF, Azab SS. Anti-inflammatory and immunomodulatory effects of Spirulina platensis in comparison to Dunaliella salina in acetic acid-induced rat experimental colitis Immunopharmacol Immunotoxicol. 2015 Jan 08 [PMID: 25567297] (IHC-P, Rat)

Details:
COX-2 antibody used for IHC-P on sections of colonic lesions from Rats subjected to acetic acid-induced experimental colitis followed by treatment or not with Spirulina platensis/SP, Dunaliella salina/DS extracts or Sulfasalazine/SSZ (standard drug used to treat bowel inflammation, diarrhea, rectal bleeding, and abdominal pain in patients with ulcerative colitis). The acetic acid-treated group depicted severe immunoreactiivity of COX2 in mucosa, submucosa and muscularis, while the control as well as the ones treated with SP or SSZ had no COX2 expression. Dunaliella-treated/DS group however had considerable levels of COX2 expression in mucosa and submucosa layers of the colon tissue (Figure 5). Spirulina platensis exerted superior beneficial effects in alleviating acetic acid-induced colitis via anti-inflammatory actions which were even better than SSZ.
IHC-P Rat
Yuan C, Smith WL. A Cyclooxygenase-2 Dependent Prostaglandin E2 Biosynthetic System in the Golgi Apparatus. J. Biol. Chem. 2014 Dec 29 [PMID: 25548276] (WB, Human, Mouse) WB Human, Mouse
Yara S, Lavoie JC, Beaulieu JF et al. Iron-Ascorbate-Mediated Lipid Peroxidation Causes Epigenetic Changes in the Antioxidant Defense in Intestinal Epithelial Cells: Impact on Inflammation. PLoS One 2013 May 22 [PMID: 23717425] (WB, Human) WB Human
Denis MC, Furtos A, Dudonne S et al. Apple Peel Polyphenols and Their Beneficial Actions on Oxidative Stress and Inflammation. PLoS One 2013 [PMID: 23372666] (WB, Human) WB Human
Kogiso M, Shinohara T, Dorey CK, Shibata Y. Cholera toxin induces a shift from inactive to active COX-2 in alveolar macrophages activated by Mycobacterium bovis BCG Infect Immun 2012 Nov 12 [PMID: 23147035] (WB, Mouse) WB Mouse
Wu MR, Hou MH, Lin YL, Kuo CF. 2,4,5-TMBA, a Natural Inhibitor of Cyclooxygenase-2, Suppresses Adipogenesis and Promotes Lipolysis in 3T3-L1 Adipocytes J Agric Food Chem 2012 Jul 25 [PMID: 22746947] (WB, Mouse) WB Mouse
Show All 16 Publications.

Review for COX-2 Antibody (NB100-689) (1) 21

Average Rating: 2
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB100-689:
Filter by Applications
Simple Western
(1)
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Filter by Species
Rat
(1)
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Images Ratings Applications Species Date Details
Simple Western COX-2 NB100-689
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reviewed by:
Anonymous
Simple Western Rat 02/19/2015
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Summary

ApplicationSimple Western
Sample TestedNon membrane associated protein lysate from rat brains
SpeciesRat

Blocking

Blocking DetailsHeat block samples at 95 degrees C for 5 minutes

Primary Anitbody

Dilution Ratio30 minutes

Secondary Antibody

Secondary Description30 minute incubation

Details

Detection NotesWes

Comments

CommentsSuggested dilution has been formatted and added to datasheet image alongside other applications.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for COX-2 Antibody (NB100-689). (Showing 1 - 1 of 1 FAQs).

  1. Can you please tell me the concentration for product NB100-689 (Lot #Y295)?
    • The concentration for the lot# Y295 of NB100-689 (COX-2 Antibody) is 0.08 mg/ml.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for COX-2 Antibody (NB100-689)

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Diseases for COX-2 Antibody (NB100-689)

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Pathways for COX-2 Antibody (NB100-689)

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PTMs for COX-2 Antibody (NB100-689)

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Research Areas for COX-2 Antibody (NB100-689)

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Blogs on COX-2.

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Inhibitor kappa B-alpha (IkappaB-alpha)
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Recent Reviews

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Anonymous
02/19/2015
Application: Simple Western
Species: Rat

Bioinformatics

Gene Symbol PTGS2
Entrez
Uniprot