COX-2 Antibody

Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689] - Formalin fixed paraffin embedded colon carcinoma stained with COX-2 antibody (NB100-689).

Simple Western: COX-2 Antibody [NB100-689] - Simple Western lane view shows a specific band for COX2 in 1.0 mg/ml of Rat Brain at an antibody concentration of 1:100. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image provided through customer review.

• Reactivity: Hu, Rt
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

COX-2 Antibody Summary

• Immunogen: Recombinant protein corresponding to the C-terminus of rat COX2 (the exact amino acid sequence is considered to be proprietary information).
• Localization: Microsomal membrane, cytoplasmic
• Specificity: This reacts with the 70 kDa protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PTGS2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Simple Western 1:100
  • Western Blot 1:100-1:2000
• Application Notes: IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 31592103).
• Control:

  Human Lung Whole Tissue Lysate (Adult Whole Tumor)
  Human Colon Whole Tissue Lysate (Adult Whole Tumor)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4), 0.2% BSA, Tween-20
• Preservative: 0.05% Sodium Azide
• Purity: Affinity purified

Alternate Names for COX-2 Antibody

• COX2
• COX-2
• COX2cyclooxygenase 2b
• cyclooxygenase-2
• EC 1.14.99
• EC 1.14.99.1
• GRIPGHS
• hCox-2
• PGG/HS
• PGH synthase 2
• PGHS-2
• PHS II
• PHS-2
• PHS-II
• prostaglandin G/H synthase 2
• prostaglandin G/H synthase and cyclooxygenase
• Prostaglandin H2 synthase 2
• prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase andcyclooxygenase)
• Prostaglandin-endoperoxide synthase 2
• PTGS2

Background

COX2 (Cyclooxygenase 2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initally identified as immediate early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for COX-2 Antibody (NB100-689)(34)

Publications using NB100-689

• Lin FL, Cheng YW, Chen LH et al. Retinal protection by fungal product theissenolactone B in a sodium iodate-induced AMD model through targeting retinal pigment epithelial matrix metalloproteinase-9 and microglia activity Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2022-12-17 [PMID: 36535199] (WB, Mouse)
• PeNa-Mercado E, Garcia-Lorenzana M, Huerta-Yepez S et al. Effect of melatonin on electrical impedance and biomarkers of damage in a gastric ischemia/reperfusion model PloS one 2022-08-16 [PMID: 35972989] (IHC-P, Rat)
  
  Details:
  Dilution used for IHC-P 1:1000
• Jayakumar T, Yang CM, Yen TL et al. Anti-Inflammatory Mechanism of An Alkaloid Rutaecarpine in LTA-Stimulated RAW 264.7 Cells: Pivotal Role on NF-kappaB and ERK/p38 Signaling Molecules International journal of molecular sciences 2022-05-24 [PMID: 35682568] (WB, Mouse)
• Ham HJ, Lee YS, Lee HP et al. G721-0282 Exerts Anxiolytic-Like Effects on Chronic Unpredictable Mild Stress in Mice Through Inhibition of Chitinase-3-Like 1-Mediated Neuroinflammation Frontiers in cellular neuroscience 2022-03-07 [PMID: 35345530] (WB, Mouse)
• Balde A, Raghavender P, Dasireddy S et al. Crab Pentapeptide and Its Anti-inflammatory Activity on Macrophage Cells International Journal of Peptide Research and Therapeutics 2021-09-08 (WB)
• Eduardo P, Mario G, Carlos CEsar P et al. Bioelectric, tissue, and molecular characteristics of the gastric mucosa at different times of ischemia Experimental Biology and Medicine 2021-06-15 [PMID: 34130514]
• Ham HJ, Lee YS, Yun J et al. K284-6111 alleviates memory impairment and neuroinflammation in Tg2576 mice by inhibition of Chitinase-3-like 1 regulating ERK-dependent PTX3 pathway Aging (Albany NY) 2020-11-07 [PMID: 33222690] (IF/IHC, Human)
• Joshi I, Mohideen HS, Nazeer RA A Meretrix meretrix visceral mass derived peptide inhibits lipopolysaccharide-stimulated responses in RAW264.7 cells and adult zebrafish model Int Immunopharmacol 2020-11-10 [PMID: 33187909]
• Jain S, Samal AG, Das B et al. Escherichia coli, a common constituent of benign prostate hyperplasia-associated microbiota induces inflammation and DNA damage in prostate epithelial cells Prostate 2020-08-24 [PMID: 32835423]
• Park Kr, Hwang Cj, Yun Hm et Al. Prevention of multiple system atrophy using human bone marrow-derived mesenchymal stem cells by reducing polyamine and cholesterol-induced neural damages Stem Cell Res Ther 2020-03-04 [PMID: 32127052] (WB, Rat)
• Arda-Pirincci P, Sacan O, Ozal-Coskun C et al. Galectin-1 exhibits a protective effect against hepatotoxicity induced by dextran sulfate sodium in mice Hum Exp Toxicol 2019-12-01 [PMID: 31789064] (WB)
• Dileepan M, Rastle-Simpson S, Greenberg Y et al. Effect Of Dual sEH/COX-2 Inhibition on Allergen-Induced Airway Inflammation Front Pharmacol 2019-09-27 [PMID: 31611798] (IF/IHC, Mouse)
• Ham HJ, Han SB, Yun J et al. Bee venom phospholipase A2 ameliorates amyloidogenesis and neuroinflammation through inhibition of signal transducer and activator of transcription-3 pathway in Tg2576 mice Transl Neurodegener 2019-10-02 [PMID: 31592103] (IHC-Fr, Mouse)
• Choi JY, Yun J, Hwang CJ et al. (E)-2-methoxy-4-(3-(4-methoxyphenyl) prop-1-en-1-yl) Phenol Ameliorates MPTP-Induced Dopaminergic Neurodegeneration by Inhibiting the STAT3 Pathway Int J Mol Sci 2019-05-29 [PMID: 31146332] (WB, Rat)
• Sukumaran V, Tsuchimochi H et al. Liraglutide Improves Renal Endothelial Function in Obese Zucker Rats on a High-Salt Diet. J Pharmacol Exp Ther 2019-01-06 [PMID: 30910920] (WB, Rat)
• Gericke A, Wolff I, Musayeva A et al. Retinal arteriole reactivity in mice lacking the endothelial nitric oxide synthase (eNOS) gene Exp. Eye Res. 2019-02-01 [PMID: 30716330] (IHC-P, Mouse)
• Fong LY, Jing R, Smalley KJ et al. Human-like hyperplastic prostate with low ZIP1 induced solely by Zn deficiency in rats. Proc. Natl. Acad. Sci. U.S.A. 2018-11-20 [PMID: 30397150] (IHC-P, Rat)
• Suyama K, Sakai D, Hirayama N et al. Effects of interleukin-17A in nucleus pulposus cells and its small-molecule inhibitors for intervertebral disc disease. J. Cell. Mol. Med. 2018-09-11 [PMID: 30207057] (WB, Human)
• Boisvert NC, Holterman CE, Thibodeau JF et al. Hyperfiltration in Ubiquitin C-Terminal Hydrolase L1 -Deleted Mice Clin. Sci. 2018-05-08 [PMID: 29739827] (Mouse)
• Jung Yu Yeon, Son Dong Ju, Lee Hye Lim et al. Loss of Parkin reduces inflammatory arthritis by inhibiting p53 degradation. Redox Biology 2017-01-01 [PMID: 28395174] (WB, Mouse)
• Grimmig T, Matthes N, Hoeland K et al. TLR7 and TLR8 expression increases tumor cell proliferation and promotes chemoresistance in human pancreatic cancer Int. J. Oncol. 2015-07-02 [PMID: 26134824] (WB, Human)
  
  Details:
  COX-2 antibody was used for WB analysis of lysates from human normal pancreatic tissues as well as pancreatic cancer tissue samples (Figure 4).
• Torricelli F, Mandato VD, Farnetti E et al. Polymorphisms in cyclooxygenase-2 gene in endometrial cancer patients Tumour Biol. 2015-04-22 [PMID: 25900875]
• Abdel-Daim MM, Farouk SM, Madkour FF, Azab SS. Anti-inflammatory and immunomodulatory effects of Spirulina platensis in comparison to Dunaliella salina in acetic acid-induced rat experimental colitis Immunopharmacol Immunotoxicol. 2015-01-08 [PMID: 25567297] (IHC-P, Rat)
  
  Details:
  iNOS antibody used for IHC-P on sections of colonic lesions from Rats subjected to acetic acid-induced experimental colitis followed by treatment or not with Spirulina platensis/SP, Dunaliella salina/DS extracts or Sulfasalazine/SSZ (standard drug used to treat bowel inflammation, diarrhea, rectal bleeding, and abdominal pain in patients with ulcerative colitis). The acetic acid-treated group as well as Dunaliella-treated/DS groups of rats showed severe immunoreactiivity of iNOS in mucosa, submucosa and muscularis, whereas, the control as well as the ones treated with SP or SSZ had weak to moderate iNOS expression. (Figure 6). Spirulina platensis exerted superior beneficial effects in alleviating acetic acid-induced colitis via anti-inflammatory actions which were even better than SSZ.
• Yuan C, Smith WL. A Cyclooxygenase-2 Dependent Prostaglandin E2 Biosynthetic System in the Golgi Apparatus. J. Biol. Chem. 2014-12-29 [PMID: 25548276] (WB, Mouse, Human)
• Yara S, Lavoie JC, Beaulieu JF et al. Iron-Ascorbate-Mediated Lipid Peroxidation Causes Epigenetic Changes in the Antioxidant Defense in Intestinal Epithelial Cells: Impact on Inflammation. PLoS One 2013-05-22 [PMID: 23717425] (WB, Human)
• Denis MC, Furtos A, Dudonne S et al. Apple Peel Polyphenols and Their Beneficial Actions on Oxidative Stress and Inflammation. PLoS One 2013-01-01 [PMID: 23372666] (WB, Human)
• Kogiso M, Shinohara T, Dorey CK, Shibata Y. Cholera toxin induces a shift from inactive to active COX-2 in alveolar macrophages activated by Mycobacterium bovis BCG Infect Immun 2012-11-12 [PMID: 23147035] (WB, Mouse)
• Wu MR, Hou MH, Lin YL, Kuo CF. 2,4,5-TMBA, a natural inhibitor of cyclooxygenase-2, suppresses adipogenesis and promotes lipolysis in 3T3-L1 adipocytes.. J Agric Food Chem. 2012-07-25 [PMID: 22746947] (WB, Mouse)
• Kogiso M, Shinohara T, Dorey CK, Shibata Y. Role of PPARgamma in COX-2 Activation in Mycobacterial Pulmonary Inflammation Inflammation 2012-06-14 [PMID: 22696146] (WB, Mouse)
• Liu YC, Ho HC, Lee MR et al. Early induction of cytokines/cytokine receptors and Cox2, and activation of NF-kB in 4-nitroquinoline 1-oxide-induced murine oral cancer model. Toxicol Appl Pharmacol. 2012-07-15 [PMID: 22561872] (IF/IHC, Mouse)
• Neilson AP, Djuric Z, Ren J et al. Effect of cyclooxygenase genotype and dietary fish oil on colonic eicosanoids in mice. The Journal of Nutritional Biochemistry. 2011-09-19 [PMID: 21937210]
• Wada M, Saunders TL, Morrow J et al. Two pathways for cyclooxygenase-2 protein degradation in vivo. J Biol Chem;284(45):30742-53. 2009-11-06 [PMID: 19758985]
• Chu P-H, Yeh L-K, Lin H-C et al. Deletion of the FHL2 Gene Attenuating Neovascularization after Corneal Injury. Invest Ophthalmol Vis Sci;49(12):5314-5318. 2008-01-01 [PMID: 18708619]
• Paiotti AP, Artigiani Neto R, Forones NM et al. Immunoexpression of cyclooxygenase-1 and -2 in ulcerative colitis. Braz J Med Biol Res 2007-07-01 [PMID: 17653443]

Review for COX-2 Antibody (NB100-689) (1)

Average Rating: 2

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Anonymous Simple Western Rat 02/19/2015

Summary

• Application: Simple Western
• Sample Tested: Non membrane associated protein lysate from rat brains
• Species: Rat

FAQs for COX-2 Antibody (NB100-689). (Showing 1 - 1 of 1 FAQs).

1. Can you please tell me the concentration for product NB100-689 (Lot #Y295)?
   • The concentration for the lot# Y295 of NB100-689 (COX-2 Antibody) is 0.08 mg/ml.

Bioinformatics

• Gene Symbol: PTGS2
• Entrez:
  • 5743(Human)
  • 19225(Mouse)
  • 29527(Rat)
• Uniprot:
  • P35355(Rat)