COX-2 Antibody

Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689] - Formalin fixed paraffin embedded colon carcinoma stained with COX-2 antibody.

Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689] - Formalin fixed paraffin embedded human colon carcinoma stained with COX2 antibody.

Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689] - Formalin fixed paraffin embedded human colon carcinoma stained with COX2 antibody.

Simple Western: COX-2 Antibody [NB100-689] - Simple Western lane view shows a specific band for COX2 in 1.0 mg/ml of Rat Brain at an antibody concentration of 1:100. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image provided through customer review.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

COX-2 Antibody Summary

• Immunogen: Recombinant protein corresponding to the C-terminus of rat COX2 (the exact amino acid sequence is considered to be proprietary information).
• Localization: Microsomal membrane, cytoplasmic
• Specificity: This reacts with the 70 kDa protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PTGS2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
  • Simple Western 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:100
• Application Notes: Immunohistochemistry (paraffin-embedded sections): Use at a dilution of 1/50-1/100* for 10 min at RT. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
• Positive Control:

  Lung Lysate (NB820-59432)
  Colon Lysate (NB820-59425)
  COX-2 Lysate (NBP2-66242)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS with 2% BSA
• Preservative: 10mM Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for COX-2 Antibody

• COX2
• COX-2
• COX2cyclooxygenase 2b
• cyclooxygenase-2
• EC 1.14.99
• EC 1.14.99.1
• GRIPGHS
• hCox-2
• PGG/HS
• PGH synthase 2
• PGHS-2
• PHS II
• PHS-2
• PHS-II
• prostaglandin G/H synthase 2
• prostaglandin G/H synthase and cyclooxygenase
• Prostaglandin H2 synthase 2
• prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase andcyclooxygenase)
• Prostaglandin-endoperoxide synthase 2
• PTGS2

Background

COX2 (Cyclooxygenase 2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initally identified as immediate early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for COX-2 Antibody (NB100-689)(15)

Publications using NB100-689

• Boisvert NC, Holterman CE, Thibodeau JF et al. Hyperfiltration in Ubiquitin C-Terminal Hydrolase L1 -Deleted Mice Clin. Sci. May 8 2018 [PMID: 29739827] (Mouse)
• Grimmig T, Matthes N, Hoeland K et al. TLR7 and TLR8 expression increases tumor cell proliferation and promotes chemoresistance in human pancreatic cancer Int. J. Oncol. 2015 Jul 02 [PMID: 26134824] (WB, Human)
  
  Details:
  COX-2 antibody was used for WB analysis of lysates from human normal pancreatic tissues as well as pancreatic cancer tissue samples (Figure 4).
• Torricelli F, Mandato VD, Farnetti E et al. Polymorphisms in cyclooxygenase-2 gene in endometrial cancer patients Tumour Biol. 2015 Apr 22 [PMID: 25900875]
• Abdel-Daim MM, Farouk SM, Madkour FF, Azab SS. Anti-inflammatory and immunomodulatory effects of Spirulina platensis in comparison to Dunaliella salina in acetic acid-induced rat experimental colitis Immunopharmacol Immunotoxicol. 2015 Jan 08 [PMID: 25567297] (IHC-P, Rat)
  
  Details:
  COX-2 antibody used for IHC-P on sections of colonic lesions from Rats subjected to acetic acid-induced experimental colitis followed by treatment or not with Spirulina platensis/SP, Dunaliella salina/DS extracts or Sulfasalazine/SSZ (standard drug used to treat bowel inflammation, diarrhea, rectal bleeding, and abdominal pain in patients with ulcerative colitis). The acetic acid-treated group depicted severe immunoreactiivity of COX2 in mucosa, submucosa and muscularis, while the control as well as the ones treated with SP or SSZ had no COX2 expression. Dunaliella-treated/DS group however had considerable levels of COX2 expression in mucosa and submucosa layers of the colon tissue (Figure 5). Spirulina platensis exerted superior beneficial effects in alleviating acetic acid-induced colitis via anti-inflammatory actions which were even better than SSZ.
• Yuan C, Smith WL. A Cyclooxygenase-2 Dependent Prostaglandin E2 Biosynthetic System in the Golgi Apparatus. J. Biol. Chem. 2014 Dec 29 [PMID: 25548276] (WB, Human, Mouse)
• Yara S, Lavoie JC, Beaulieu JF et al. Iron-Ascorbate-Mediated Lipid Peroxidation Causes Epigenetic Changes in the Antioxidant Defense in Intestinal Epithelial Cells: Impact on Inflammation. PLoS One 2013 May 22 [PMID: 23717425] (WB, Human)
• Denis MC, Furtos A, Dudonne S et al. Apple Peel Polyphenols and Their Beneficial Actions on Oxidative Stress and Inflammation. PLoS One 2013 [PMID: 23372666] (WB, Human)
• Kogiso M, Shinohara T, Dorey CK, Shibata Y. Cholera toxin induces a shift from inactive to active COX-2 in alveolar macrophages activated by Mycobacterium bovis BCG Infect Immun 2012 Nov 12 [PMID: 23147035] (WB, Mouse)
• Wu MR, Hou MH, Lin YL, Kuo CF. 2,4,5-TMBA, a Natural Inhibitor of Cyclooxygenase-2, Suppresses Adipogenesis and Promotes Lipolysis in 3T3-L1 Adipocytes J Agric Food Chem 2012 Jul 25 [PMID: 22746947] (WB, Mouse)
• Kogiso M, Shinohara T, Dorey CK, Shibata Y. Role of PPARgamma in COX-2 Activation in Mycobacterial Pulmonary Inflammation Inflammation 2012 Jun 14 [PMID: 22696146] (WB, Mouse)
• Yu-Ching Liu, Heng-Chien Ho, Miau-Rong Lee, Kuang-Chi Lai, Chung-Min Yeh, Yueh-Min Lin, Tin-Yun Ho, Chien-Yun Hsiang, Jing-Gung Chung. Early induction of cytokines/cytokine receptors and Cox2, and activation of NF-?B in 4-nitroquinoline 1-oxide-induced murine oral cancer model,. Toxicology and Applied Pharmacology, 10.1016/j.taap.04.023. 2012 [PMID: 22561872] (IHC, Mouse)
• Neilson AP, Djuric Z, Ren J et al. Effect of cyclooxygenase genotype and dietary fish oil on colonic eicosanoids in mice. The Journal of Nutritional Biochemistry. 2011 Sep 19. [PMID: 21937210]
• Wada M, Saunders TL, Morrow J et al. Two pathways for cyclooxygenase-2 protein degradation in vivo. J Biol Chem;284(45):30742-53. 2009 Nov 6. [PMID: 19758985]
• Chu P-H, Yeh L-K, Lin H-C et al. Deletion of the FHL2 Gene Attenuating Neovascularization after Corneal Injury. Invest Ophthalmol Vis Sci;49(12):5314-5318. 2008 [PMID: 18708619]
• Paiotti AP, Artigiani Neto R, Forones NM et al. Immunoexpression of cyclooxygenase-1 and -2 in ulcerative colitis. Braz J Med Biol Res 2007 Jul [PMID: 17653443]

Review for COX-2 Antibody (NB100-689) (1)

Average Rating: 2

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Anonymous Simple Western Rat 02/19/2015

Summary

• Application: Simple Western
• Sample Tested: Non membrane associated protein lysate from rat brains
• Species: Rat

Blocking

• Blocking Details: Heat block samples at 95 degrees C for 5 minutes

Primary Anitbody

• Dilution Ratio: 30 minutes

Secondary Antibody

• Secondary Description: 30 minute incubation

Details

• Detection Notes: Wes

Comments

• Comments: Suggested dilution has been formatted and added to datasheet image alongside other applications.

FAQs for COX-2 Antibody (NB100-689). (Showing 1 - 1 of 1 FAQs).

1. Can you please tell me the concentration for product NB100-689 (Lot #Y295)?
   • The concentration for the lot# Y295 of NB100-689 (COX-2 Antibody) is 0.08 mg/ml.

Bioinformatics

• Gene Symbol: PTGS2
• Entrez:
  • 5743(Human)
  • 19225(Mouse)
  • 29527(Rat)
• Uniprot:
  • P35355(Rat)