COX-2 Antibody

Images

 
Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689] - Formalin fixed paraffin embedded colon carcinoma stained with COX-2 antibody (NB100-689).
Simple Western: COX-2 Antibody [NB100-689] - Simple Western lane view shows a specific band for COX2 in 1.0 mg/ml of Rat Brain at an antibody concentration of 1:100. This experiment was performed under reducing ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

COX-2 Antibody Summary

Immunogen
Recombinant protein corresponding to the C-terminus of rat COX2 (the exact amino acid sequence is considered to be proprietary information).
Localization
Microsomal membrane, cytoplasmic
Specificity
This reacts with the 70 kDa protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PTGS2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Simple Western 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:100
Application Notes
Immunohistochemistry (paraffin-embedded sections): Use at a dilution of 1/50-1/100* for 30 min at RT. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 31592103).
Control
Human Lung Whole Tissue Lysate (Adult Whole Tumor)
Human Colon Whole Tissue Lysate (Adult Whole Tumor)
COX-2 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 2
using
NB100-689 in the following applications:

Publications
Read Publications using
NB100-689 in the following applications:

  • IHC
    2 publications
  • 1 publication
  • 3 publications
  • WB
    12 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4) and 0.2% BSA
Preservative
0.09% Sodium Azide
Purity
Affinity purified

Alternate Names for COX-2 Antibody

  • COX2
  • COX-2
  • COX2cyclooxygenase 2b
  • cyclooxygenase-2
  • EC 1.14.99
  • EC 1.14.99.1
  • GRIPGHS
  • hCox-2
  • PGG/HS
  • PGH synthase 2
  • PGHS-2
  • PHS II
  • PHS-2
  • PHS-II
  • prostaglandin G/H synthase 2
  • prostaglandin G/H synthase and cyclooxygenase
  • Prostaglandin H2 synthase 2
  • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase andcyclooxygenase)
  • Prostaglandin-endoperoxide synthase 2
  • PTGS2

Background

COX2 (Cyclooxygenase 2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initally identified as immediate early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-Fr, IHC-P

Publications for COX-2 Antibody (NB100-689)(24)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: IHC, IHC-Fr, IHC-P, WB.


Filter By Application
IHC
(2)
IHC-Fr
(1)
IHC-P
(3)
WB
(12)
All Applications
Filter By Species
Human
(5)
Mouse
(10)
Rat
(4)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using NB100-689 Applications Species
Park Kr, Hwang Cj, Yun Hm et Al. Prevention of multiple system atrophy using human bone marrow-derived mesenchymal stem cells by reducing polyamine and cholesterol-induced neural damages Stem Cell Res Ther Mar 4 2020 [PMID: 32127052] (WB, Rat) WB Rat
Arda-Pirincci P, Sacan O, Ozal-Coskun C et al. Galectin-1 exhibits a protective effect against hepatotoxicity induced by dextran sulfate sodium in mice Hum Exp Toxicol Dec 1 2019 [PMID: 31789064] (WB) WB
Dileepan M, Rastle-Simpson S, Greenberg Y et al. Effect Of Dual sEH/COX-2 Inhibition on Allergen-Induced Airway Inflammation Front Pharmacol 2019 Sep 27 [PMID: 31611798] (IHC, Mouse) IHC Mouse
Ham HJ, Han SB, Yun J et al. Bee venom phospholipase A2 ameliorates amyloidogenesis and neuroinflammation through inhibition of signal transducer and activator of transcription-3 pathway in Tg2576 mice Transl Neurodegener Oct 2 2019 [PMID: 31592103] (IHC-Fr, Mouse) IHC-Fr Mouse
Choi JY, Yun J, Hwang CJ et al. (E)-2-methoxy-4-(3-(4-methoxyphenyl) prop-1-en-1-yl) Phenol Ameliorates MPTP-Induced Dopaminergic Neurodegeneration by Inhibiting the STAT3 Pathway Int J Mol Sci May 29 2019 [PMID: 31146332] (WB, Rat) WB Rat
Gericke A, Wolff I, Musayeva A et al. Retinal arteriole reactivity in mice lacking the endothelial nitric oxide synthase (eNOS) gene Exp. Eye Res. Feb 1 2019 [PMID: 30716330] (IHC-P, Mouse) IHC-P Mouse
Fong LY, Jing R, Smalley KJ et al. Human-like hyperplastic prostate with low ZIP1 induced solely by Zn deficiency in rats. Proc. Natl. Acad. Sci. U.S.A. Nov 20 2018 [PMID: 30397150] (IHC-P, Rat) IHC-P Rat
Suyama K, Sakai D, Hirayama N et al. Effects of interleukin-17A in nucleus pulposus cells and its small-molecule inhibitors for intervertebral disc disease. J. Cell. Mol. Med. Sep 11 2018 [PMID: 30207057] (WB, Human) WB Human
Boisvert NC, Holterman CE, Thibodeau JF et al. Hyperfiltration in Ubiquitin C-Terminal Hydrolase L1 -Deleted Mice Clin. Sci. May 8 2018 [PMID: 29739827] (Mouse) Mouse
Jung Yu Yeon, Son Dong Ju, Lee Hye Lim et al. Loss of Parkin reduces inflammatory arthritis by inhibiting p53 degradation. Redox Biology 2017 [PMID: 28395174] (WB, Mouse) WB Mouse
Show All 24 Publications.

Review for COX-2 Antibody (NB100-689) (1) 21

Average Rating: 2
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB100-689:
Filter by Applications
Simple Western
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western COX-2 NB100-689
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reviewed by:
Anonymous
Simple Western Rat 02/19/2015
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Summary

ApplicationSimple Western
Sample TestedNon membrane associated protein lysate from rat brains
SpeciesRat

Blocking

Blocking DetailsHeat block samples at 95 degrees C for 5 minutes

Primary Anitbody

Dilution Ratio30 minutes

Secondary Antibody

Secondary Description30 minute incubation

Details

Detection NotesWes

Comments

CommentsSuggested dilution has been formatted and added to datasheet image alongside other applications.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for COX-2 Antibody (NB100-689). (Showing 1 - 1 of 1 FAQs).

  1. Can you please tell me the concentration for product NB100-689 (Lot #Y295)?
    • The concentration for the lot# Y295 of NB100-689 (COX-2 Antibody) is 0.08 mg/ml.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for COX-2 Antibody (NB100-689)

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Diseases for COX-2 Antibody (NB100-689)

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Pathways for COX-2 Antibody (NB100-689)

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Research Areas for COX-2 Antibody (NB100-689)

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Recent Reviews

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2
1
1
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Anonymous
02/19/2015
Application: Simple Western
Species: Rat

Bioinformatics

Gene Symbol PTGS2
Entrez
Uniprot