HO-1/HMOX1/HSP32 Antibody (HO-1-1) - BSA Free

Western Blot: HO-1/HMOX1/HSP32 Antibody (HO-1-1) [NBP1-97507] - Lane 1: MW marker; Lane 2: HO-1 (rat), (recombinant); Lane 3: HO-1 (human), (recombinant); Lane 4: HO-2 (human), (recombinant); Lane 5: MDBK cell lysate; Lane 6: Dog liver microsome; Lane 7: Mouse liver microsome.

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (HO-1-1) [NBP1-97507] - Analysis of human spleen tissue stained with HO-1, mAb (HO-1-1) at 10ug/ml.

Flow Cytometry: HO-1/HMOX1/HSP32 Antibody (HO-1-1) [NBP1-97507] - Analysis of 10^6 Jurkat cells stained using HO-1 monoclonal antibody (HO-1-1), (Prod. No. ADI-OSA-110), at a concentration of 50 ug/mL

Effect of Sepsis with Isoflurane or Propofol anesthesia on Liver HIF1a, Bcl2, iNOS and HO-1, Protein expression levels. (A) HIF-1 alpha protein was increased in both anesthetic groups by sepsis (Isoflurane +Sepsis, **p = 0.004 and Propofol + Sepsis, *p = 0.04). (B) Bcl-2 protein was increased in Isoflurane +Sepsis group (**p = 0.004) and no significant change in Propofol + Sepsis (*p = 0.104). (C) Inducible nitric oxide synthase [iNOS, (**p = 0.002)] and (D) Heme oxygenase-1 (HO-1, ***p = 0.0006) was increased in Isoflurane +Sepsis group. Whereas, in animals anesthetized with Propofol, three organ-protective proteins, Bcl-2, iNOS, and HO-1 expression was unchanged significantly. Data are shown in mean +/- S.D, n = 12. *denotes a statistically significant (P ⩽ 0.05) difference between animals exposed to sepsis and controls. Statistical differences between results were evaluated using the t-test. “ns

Protein expression data (upper) and representative raw data (lower) for animals anesthetized with isoflurane (bcl-2 [a median + IQR], HIF-1 alpha [b mean + 95% confidence interval (CI)], HO-1 [c mean + 95% CI for isoflurane, median + IQR for propofol], iNOS [d mean + 95% CI for isoflurane, median + IQR for propofol) *denotes a statistically significant difference between animals exposed to sepsis and controls Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31307382), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

KEAP1 modulates ferroptosis through NRF2/HO‐1 pathway in hiPSC‐derived cINs. (A) Western blot showed the expression of NRF2 and HO‐1. (B) Quantification of Fe2⁺ levels, using an iron content determination assay. (C) Measurement of MDA levels using an MDA assay kit. (D) Quantification of GSH levels using a GSH assay kit. (E) Assessment of GPX4 activity using an ELISA kit. (F) GPX4 mRNA and protein were evaluated by qRT‐PCR and Western blot analysis, respectively. (G) Measurement of lipid oxidation using C11‐BODIPY staining, a dye specific for detecting lipid reactive oxygen species. (H) Quantification of inflammatory cytokines in the cell culture supernatant using ELISA kits. *p < 0.05 and **p < 0.01 show statistical significance when compared to SCZ‐pcDNA KEAP1 group. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40021790), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

ARE-primed PD-MSCs increased antioxidant and anti-inflammatory effects in ARPE19 cells. (A) Oxidative stress levels evaluated in ARPE19 cells using MitoSOX/MitoTracker staining and (B) intensity quantified by image J. The gray bar, gray hatch bar, black bar, black hatch bar means ARPE19 cells with H2O2 and ARE low, ARPE19 cells with with H2O2, ARE low and PD-MSCs, ARPE19 cells with H2O2 and ARE high and ARPE19 cells with with H2O2, ARE high and PD-MSCs. The mRNA levels of (C) SOD1 and (D) HMOX1 analyzed by qRT-PCR. The protein levels of (E) SOD1 and (F) HMOX1 analyzed by Western blot. The mRNA levels of (G) TGFB1, (H) IL6 analyzed by qRT-PCR. The protein levels of (I) TGFB1 and (J) IL6 analyzed by Western blot. Scale bar = 200 μm. Each experiment was replicated three times, resulting in a total sample size of three (n = 3). The data represent the mean +/- SEM. Statistical significance was determined by using one-way ANOVA and Tukey’s post hoc test for the comparison of groups, * p < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39056810), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, Simple Western, Flow, ICC/IF, IHC
• Clone: HO-1-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

HO-1/HMOX1/HSP32 Antibody (HO-1-1) - BSA Free Summary

• Description: Novus Biologicals Mouse HO-1/HMOX1/HSP32 Antibody (HO-1-1) - BSA Free (NBP1-97507) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and Simple Western. Anti-HO-1/HMOX1/HSP32 Antibody: Cited in 20 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to the sequence near the N-terminus of human HO-1.
• Marker: Oxidative Stress Marker
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HMOX1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Simple Western
  • Western Blot 1:1000
• Application Notes: Use in Simple Western reported in scientific literature (PMID: 29890265).
  See Simple Western Antibody Database for Simple Western validation: separated by Size
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for HO-1/HMOX1/HSP32 Antibody (HO-1-1) - BSA Free

• bK286B10
• EC 1.14.99.3
• heat shock protein, 32-kD
• heme oxygenase (decycling) 1
• HMOX1
• HO
• HO1
• HO-1
• HO-1heme oxygenase 1
• HSP32

Background

Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HO-1/HMOX1/HSP32 Antibody (NBP1-97507)(20)

Publications using NBP1-97507

• Peng R, Liu X, Wang C et al. Iron overload enhances TBI-induced cardiac dysfunction by promoting ferroptosis and cardiac inflammation Biochemical and biophysical research communications 2023-11-19 [PMID: 37801989] (IHC, Mouse)
• Hung YW, Ouyang C, Ping X et al. Extracellular arginine availability modulates eIF2? O-GlcNAcylation and heme oxygenase 1 translation for cellular homeostasis Journal of biomedical science 2023-05-22 [PMID: 37217939] (IHC-P, WB, ICC/IF, Human)
• Yoo MH, Lee SJ, Kim W et al. Bisphenol A impairs renal function by reducing Na+/K+-ATPase and F-actin expression, kidney tubule formation in vitro and in vivo Ecotoxicology and environmental safety 2022-10-04 [PMID: 36206637] (IF/IHC, ICC/IF, Rat, Human)
• Rai D, Tripathi AK, Sardar A et al. A novel BMP2 secretagogue ameliorates glucocorticoid induced oxidative stress in osteoblasts by activating NRF2 dependent survival while promoting Wnt/ beta-catenin mediated osteogenesis Free radical biology & medicine 2022-08-10 [PMID: 35963563] (WB, Mouse)
• Arigela CS, Nelli G, Gan SH Et al. Bitter Gourd Honey Ameliorates Hepatic and Renal Diabetic Complications on Type 2 Diabetes Rat Models by Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Mechanisms Foods (Basel, Switzerland) 2021-11-20 [PMID: 34829154] (ICC/IF, Rat)
• Vendidandala NR, Yin TP, Nelli G Et al. Gallocatechin silver nanoparticle impregnated cotton gauze patches enhance wound healing in diabetic rats by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-kappa B pathways Life sciences 2021-10-06 [PMID: 34624322] (IHC-P, Rat)
• Nesbitt NM, Malone LE, Liu Z et al. Divergent erythroid megakaryocyte fates in Blvrb-deficient mice establish non-overlapping cytoprotective functions during stress hematopoiesis Free radical biology & medicine 2020-12-24 [PMID: 33359909] (WB, Mouse)
• Seimetz M, Sommer N, Bednorz M et al. NADPH oxidase subunit NOXO1 is a target for emphysema treatment in COPD Nat Metab 2020-06-01 [PMID: 32694733]
• Seok J, Park H, Choi JH et al. Placenta-Derived Mesenchymal Stem Cells Restore the Ovary Function in an Ovariectomized Rat Model via an Antioxidant Effect Antioxidants (Basel) 2020-07-06 [PMID: 32640638] (WB, Rat)
• Kaya O, Orhan E, Sapmaz-Metin M, et al. The effects of epidermal growth factor on early burn-wound progression in rats Dermatol Ther 2019-12-18 [PMID: 31849151] (IF/IHC, Rat)
• Deng G, Ma C, Zhao H et al. Anti-edema and antioxidant combination therapy for ischemic stroke via glyburide-loaded betulinic acid nanoparticles Theranostics 2019-09-21 [PMID: 31660082] (WB, Human)
• Tocmo R, Parkin K S-1-propenylmercaptocysteine protects murine hepatocytes against oxidative stress via persulfidation of Keap1 and activation of Nrf2 Free Radic. Biol. Med. 2019-07-23 [PMID: 31349040] (WB, Mouse)
• Mossoba ME, Vohra S, Toomer H et al. Diglycolic acid induces HepG2/C3A liver cell toxicity in vitro Toxicol In Vitro. [PMID: 29890265] (Simple Western, Human)
• Dey S, Sayers CM, Verginadis II et al. ATF4-dependent induction of heme oxygenase 1 prevents anoikis and promotes metastasis. J Clin Invest 2015-07-01 [PMID: 26011642] (IF/IHC)
• Jang KJ, Mano H, Aoki K et al. Mitochondrial function provides instructive signals for activation-induced B-cell fates Nat Commun. Nat Commun 2015-04-10 [PMID: 25857523] (WB, Mouse)
  
  Details:
  Using the DyLight 650 conjugated version of NB600-302, catalog number NB600-302C.
• Gupta A, Lacoste B, Pistel PJ et al. Neurotherapeutic effects of novel HO-1 inhibitors in vitro and in a transgenic mouse model of Alzheimer's disease J. Neurochem. 2014-08-11 [PMID: 25111043]
• Kimbrel EA, Kouris NA, Yavanian G et al. Mesenchymal stem cell population derived from human pluripotent stem cells displays potent immunomodulatory and therapeutic properties. Stem Cells Dev 2014-03-20 [PMID: 24650034] (ICC/IF, Human)
  
  Details:
  Using the DyLight 488 conjugated version of NBP1-97507, catalog number NBP1-97507G.
• FA Arosa et al. Red blood cells upregulate cytoprotective proteins and the labile iron pool in dividing human T cells despite a reduction in oxidative stress. Free Radic. Biol. Med. 35, 1404 . 2003-01-01 [PMID: 14642388] (WB, Human)
• FR Jirik et al. Defective hematopoiesis and hepatic steatosis in mice with combined deficiencies of the genes encoding Fancc and Cu/Zn superoxide dismutase. Blood 98, 1003. 2001-01-01 [PMID: 11493445] (WB, Mouse)
• DK Das et al. Hmox-1 constitutes an adaptive response to effect antioxidant cardioprotection: a study with transgenic mice heterozygous for targeted disruption of the Heme oxygenase-1 gene. Circulation 103, 1695. 2001-01-01 [PMID: 11273999] (WB, Mouse)

Review for HO-1/HMOX1/HSP32 Antibody (NBP1-97507) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Sitara Sankar WB Mouse 05/23/2017

Summary

• Application: Western Blot
• Sample Tested: Mouse macrophages
• Species: Mouse

FAQs for HO-1/HMOX1/HSP32 Antibody (NBP1-97507). (Showing 1 - 1 of 1 FAQs).

1. What is the clone for antibody NBP1-97507?
   • The clone for NBP1-97507 is HO-1-1.

Bioinformatics

• Gene Symbol: HMOX1
• Entrez:
  • 513221(Bovine)
  • 3162(Human)
  • 24451(Mouse)
  • 15368(Rat)
• Uniprot:
  • P09601()