HO-1/HMOX1/HSP32 Antibody (HO-1-1)

Images

 
Western Blot: HO-1/HMOX1/HSP32 Antibody (HO-1-1) [NBP1-97507] - Lane 1: MW marker; Lane 2: HO-1 (rat), (recombinant); Lane 3: HO-1 (human), (recombinant); Lane 4: HO-2 (human), (recombinant); Lane 5: MDBK cell lysate; ...read more
Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (HO-1-1) [NBP1-97507] - Analysis of human spleen tissue stained with HO-1, mAb (HO-1-1) at 10ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
HO-1-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HO-1/HMOX1/HSP32 Antibody (HO-1-1) Summary

Immunogen
Synthetic peptide corresponding to the sequence near the N-terminus of human HO-1.
Marker
Oxidative Stress Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HMOX1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 10 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-97507 in the following applications:

Publications
Read Publications using
NBP1-97507 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • WB
    4 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 50% Glycerol
Preservative
0.09% Sodium Azide
Purity
Protein G purified

Alternate Names for HO-1/HMOX1/HSP32 Antibody (HO-1-1)

  • bK286B10
  • EC 1.14.99.3
  • heat shock protein, 32-kD
  • heme oxygenase (decycling) 1
  • HMOX1
  • HO
  • HO1
  • HO-1
  • HO-1heme oxygenase 1
  • HSP32

Background

Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Bv, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P

Publications for HO-1/HMOX1/HSP32 Antibody (NBP1-97507)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


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ICC/IF
(1)
IHC
(1)
WB
(4)
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(2)
Mouse
(3)
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Showing Publications 1 - 7 of 7.
Publications using NBP1-97507 Applications Species
Dey S, Sayers CM, Verginadis II et al. ATF4-dependent induction of heme oxygenase 1 prevents anoikis and promotes metastasis. J Clin Invest 2015 Jul 1 [PMID: 26011642] (IHC) IHC
Jang KJ, Mano H, Aoki K et al. Mitochondrial function provides instructive signals for activation-induced B-cell fates Nat Commun 2015 Apr 10 [PMID: 25857523] (WB, Mouse) WB Mouse
Gupta A, Lacoste B, Pistel PJ et al. Neurotherapeutic effects of novel HO-1 inhibitors in vitro and in a transgenic mouse model of Alzheimer's disease J. Neurochem. 2014 Aug 11 [PMID: 25111043]
Kimbrel EA, Kouris NA, Yavanian G et al. Mesenchymal stem cell population derived from human pluripotent stem cells displays potent immunomodulatory and therapeutic properties. Stem Cells Dev 2014 Mar 20 [PMID: 24650034] (ICC/IF, Human)

Details:
Using the DyLight 488 conjugated version of NBP1-97507, catalog number NBP1-97507G.
ICC/IF Human
FA Arosa et al. Red blood cells upregulate cytoprotective proteins and the labile iron pool in dividing human T cells despite a reduction in oxidative stress. Free Radic. Biol. Med. 35, 1404. 2003 [PMID: 14642388] (WB, Human) WB Human
FR Jirik et al. Defective hematopoiesis and hepatic steatosis in mice with combined deficiencies of the genes encoding Fancc and Cu/Zn superoxide dismutase. Blood 98, 1003. 2001 [PMID: 11493445] (WB, Mouse) WB Mouse
DK Das et al. Hmox-1 constitutes an adaptive response to effect antioxidant cardioprotection: a study with transgenic mice heterozygous for targeted disruption of the Heme oxygenase-1 gene. Circulation 103, 1695. 2001 [PMID: 11273999] (WB, Mouse) WB Mouse

Review for HO-1/HMOX1/HSP32 Antibody (NBP1-97507) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-97507:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Sitara Sankar
WB Mouse 05/23/2017
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Summary

ApplicationWestern Blot
Sample TestedMouse macrophages
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HO-1/HMOX1/HSP32 Antibody (NBP1-97507). (Showing 1 - 1 of 1 FAQs).

  1. What is the clone for antibody NBP1-97507?
    • The clone for NBP1-97507 is HO-1-1.

Secondary Antibodies

 

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Bioinformatics Tool for HO-1/HMOX1/HSP32 Antibody (NBP1-97507)

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Recent Reviews

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Sitara Sankar
05/23/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol HMOX1
Entrez
Uniprot