HIF-2 alpha/EPAS1 Antibody [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Fi, Ha, Pm, ShSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 549

HIF-2 alpha/EPAS1 Antibody [Janelia Fluor® 549] Summary

Immunogen
A peptide derived from the C-terminus of mouse/human HIF-2 alpha protein.
Specificity
Specific for HIF-2 alpha/EPAS. Does not cross-react with HIF-1 alpha.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EPAS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Knockout Validated
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Reactivity with sheep reported in the scientific literature (PMID: 17307811). Hamster reactivity reported in scientific literature (PMID: 24997364 and 24997360)

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for HIF-2 alpha/EPAS1 Antibody [Janelia Fluor® 549]

  • Basic-helix-loop-helix-PAS protein MOP2
  • BHLHE73
  • bHLHe73ECYT4
  • Class E basic helix-loop-helix protein 73
  • endothelial PAS domain protein 1
  • endothelial PAS domain-containing protein 1
  • EPAS1
  • EPAS-1
  • HIF 2A
  • HIF-1alpha-like factor
  • HIF2 alpha
  • HIF-2 alpha
  • hif2a angiogenesis
  • HIF2A
  • HIF2AHIF-2-alpha
  • HLF
  • HLFHIF-1-alpha-like factor
  • hypoxia-inducible factor 2 alpha
  • Hypoxia-inducible factor 2-alpha
  • Member of PAS protein 2
  • MOP2
  • MOP2HIF2-alpha
  • PASD2PAS domain-containing protein 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-122JF549
Species: Hu, Mu, Rt, Fi, Ha, Pm, Sh
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KO

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for HIF-2 alpha/EPAS1 Antibody (NB100-122JF549)

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Diseases for HIF-2 alpha/EPAS1 Antibody (NB100-122JF549)

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PTMs for HIF-2 alpha/EPAS1 Antibody (NB100-122JF549)

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Blogs on HIF-2 alpha/EPAS1.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

HIF-2 alpha, Tumor Suppression and Cell Survival
HIF-2 alpha is one subunit within the HIF-2 nuclear protein that regulates cellular responses to hypoxia (low oxygen tension conditions). Hydroxylation post-translational modifications on particular HIF residues target them for degradation. Luo, et al...  Read full blog post.

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Bioinformatics

Gene Symbol EPAS1