CD31/PECAM-1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody [NBP1-71663] - Staining of mouse glioma tissue using NBP1-71663 at a dilution of 1:2500. Image provided by Dr. Eric Woolf of Dignity Health.
Western Blot: CD31/PECAM-1 Antibody [NBP1-71663] - Detection of CD31 in HUVEC cell lysates.
Simple Western: CD31/PECAM-1 Antibody [NBP1-71663] - Simple Western lane view shows a specific band for CD31/PECAM 1 in 0.5 mg/ml of HUVEC lysate. This experiment was performed under reducing conditions using the ...read more
Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody [NBP1-71663] - HUVEC cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Flow Cytometry: CD31/PECAM-1 Antibody [NBP1-71663] - CD31/PECAM1 antibody was tested in WeHi cells with Dylight 488 (green) alongside a matched isotype control (black).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CD31/PECAM-1 Antibody Summary

Immunogen
A genomic peptide made to an internal region of human CD31/PECAM1 (within residues 100-300). [Swiss-Prot P16284]
Localization
Membrane; Single-pass type I membrane protein. Cell junction. Note: Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PECAM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Simple Western 1:1000
  • Flow Cytometry 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
Application Notes
This CD31/PECAM1 antibody is useful for Immunohistochemistry, Flow Cytometry, and Western Blot, where a band is seen ~ 150 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Customer feedback has been negative on cryosections.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-71663 in the following applications:

Publications
Read Publications using
NBP1-71663 in the following applications:

  • IHC
    3 publications
  • 2 publications
  • WB
    1 publication

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for CD31/PECAM-1 Antibody

  • adhesion molecule
  • CD31 antigen
  • CD31
  • CD31/EndoCAM
  • endoCAM
  • FLJ34100
  • FLJ58394
  • GPIIA'
  • PECA1
  • PECAM1
  • PECAM-1
  • PECAM-1, CD31/EndoCAM
  • platelet endothelial cell adhesion molecule
  • platelet/endothelial cell adhesion molecule

Background

CD31/PECAM1 (cluster of differentiation 31/platelet-endothelial cell adhesion molecule-1), is a transmembrane glycoprotein belonging to immunoglobulin (Ig)-superfamily of cell adhesion molecules and is expressed by platelets, megakaryocytes, endothelial cells, certain T cells etc., and has been shown to modulate cell adhesion, endothelial cell migration and angiogenesis. CD31/PECAM-1 undergoes phosphorylation at Tyr686 through SRC family members upon versatile stimuli that includes mechanical stress and oxidative stress also, and this phosphorylation facilitates its associations with a number of SH2 domain containing binding partners such as PI3K, SHIP, PLC gamma and SHP-2. Besides, exhibiting adhesive properties, CD31/PECAM-1 implicates in angiogenesis, platelet function, and thrombosis, mechanosensing of endothelial cell response to fluid shear stress, and control of multiple stages of leukocyte migration through venular walls. Importantly, CD31/PECAM-1 is the most commonly used pan-endothelial marker for blood vessels and due to its involvement in angiogenesis and vascular permeability, it plays important role in the growth as well as metastatic spread of several cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-71663
Species: Hu, Mu
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P

Publications for CD31/PECAM-1 Antibody (NBP1-71663)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(3)
IHC-P
(2)
WB
(1)
All Applications
Filter By Species
Human
(3)
Mouse
(4)
All Species
Showing Publications 1 - 6 of 6.
Publications using NBP1-71663 Applications Species
Abraham V, Parambath A, Joe DS, DeLisser HM. Influence of PECAM-1 ligand interactions on PECAM-1-dependent cell motility and filopodia extension. Physiol Rep. 2016 Nov 1 [PMID: 27895229] (Human, Mouse)

Details:
This citation used the DyLight 650 version of this antibody.
Human, Mouse
Svenson S, Case RI, Cole RO et al. Tumor Selective Silencing Using an RNAi-Conjugated Polymeric Nanopharmaceutical. Mol. Pharm. 2016 Mar 07 [PMID: 26835715] (IHC-P, Mouse) IHC-P Mouse
Woolf EC, Curley KL, Liu Q et al. The Ketogenic Diet Alters the Hypoxic Response and Affects Expression of Proteins Associated with Angiogenesis, Invasive Potential and Vascular Permeability in a Mouse Glioma Model PLoS ONE. 2015 Jun 18 [PMID: 26083629] (WB, IHC-P, Mouse) WB, IHC-P Mouse
Lee JH, Park KS, Alberobello AT et al. The Janus Kinases Inhibitor AZD1480 Attenuates Growth of Small Cell Lung Cancers In Vitro and In Vivo. Clin Cancer Res 2013 Dec 15 [PMID: 24158701] (IHC, Human) IHC Human
Pratheeshkumar P, Son YO, Budhraja A et al. Luteolin inhibits human prostate tumor growth by suppressing vascular endothelial growth factor receptor 2-mediated angiogenesis PLoS One 2012 [PMID: 23300633] (IHC, Mouse) IHC Mouse
Pratheeshkumar P, Budhraja A, Son YO et al. Quercetin Inhibits Angiogenesis Mediated Human Prostate Tumor Growth by Targeting VEGFR- 2 Regulated AKT/mTOR/P70S6K Signaling Pathways PLoS One 2012 [PMID: 23094058] (IHC, Human) IHC Human

Reviews for CD31/PECAM-1 Antibody (NBP1-71663) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.
We have 2 reviews tested in 2 application: WB, IF.

Reviews using NBP1-71663:
Filter by Applications
WB
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot CD31/PECAM-1 NBP1-71663
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reviewed by:
Anonymous
WB Mouse 09/05/2013
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Summary

ApplicationWestern Blot
Sample TestedMouse glioma tissue and mouse normal brain tissue.
SpeciesMouse
LotA
CommentsThis antibody worked great at a reasonable dilution and required very little optimization. Our lab will continue to use this CD31 antibody and would recommend it to others studying tumor angiogenesis.

Blocking

Blocking DetailsMembrane blocked in TBST+5% BSA for 1 hour at room temperature.

Primary Anitbody

Dilution Ratio1:5000 in TBST+5% BSA overnight at 4 degrees celsius.

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, HRP conjugated
Secondary Manufacturer Cat#656120 (Life Technologies)
Secondary Concentration1:3000 in TBST+5% BSA

Details

Detection NotesECL detection, BioSpectrum Imaging System (UVP), 1 min exposure, positive controls tested = mouse glioma tissue, mouse kidney

Comments

CommentsThis antibody worked great at a reasonable dilution and required very little optimization. Our lab will continue to use this CD31 antibody and would recommend it to others studying tumor angiogenesis.
Immunofluorescence CD31/PECAM-1 NBP1-71663
Enlarge
reviewed by:
Anonymous
IF Mouse 09/05/2013
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse glioma tissue
SpeciesMouse
LotA
CommentsThis CD31 antibody worked very well in our mouse model of malignant glioma and required minimal optimization. The data gathered using this antibody gave us important information about tumor vasculature in response to anti-angiogenic therapy.

Blocking

Blocking DetailsBlocked in TBS+15% FCS+1%BSA for 1 hour at room temperature.

Primary Anitbody

Dilution Ratio1:2500 in TBS+1% BSA overnight at 4 degrees celsius

Secondary Antibody

Secondary Descriptiondonkey anti-goat IgG, Alexafluor488
Secondary Manufacturer Cat#A21206 (Life Technologies)
Secondary Concentration1:1500 in TBST+1% BSA

Details

Detection NotesImaged with Zeiss 710 LSM microscope.
Fixation DetailsFixed in 4% PFA, paraffin embedded, standard antigen retrieval, antigen retrieval in sodium citrate (pH 6.0) for 25 mins at 98C
Wash DescriptionWashed in TBST for 5 minutes, 3 washes

Comments

CommentsThis CD31 antibody worked very well in our mouse model of malignant glioma and required minimal optimization. The data gathered using this antibody gave us important information about tumor vasculature in response to anti-angiogenic therapy.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD31/PECAM-1 Antibody (NBP1-71663). (Showing 1 - 2 of 2 FAQs).

  1. I'm interested in purchasing primary CD31/PECAM1 antibody for immunohistochemistry staining on formalin fixed paraffin embedded mouse samples. You have two antibodies that would work for paraffin sections, NBP1-71663 and NB100-92205, and both are rabbit polyclonal antibody. Which one would you recommend I purchase?
    • I would recommend NBP1-71663 as the image is better than NB100-92205.
  2. I am looking for references concerning the use of NBP2-15797, NBP1-47913 and NB100-2284 in IHC on PFA- fixed section.
    • Among your mentioned catalog numbers (NBP2-15797, NBP1-47913 and NB100-2284), we have published references for NB100-2284 only. Hylander et al. 2013 (Journal of Translational Medicine 2013, 11:110) have cited the IHC-P use of NB100-2284 and this paper has also explained a nice detailed IHC-P protocol that you may follow if you want to. NBP1-71663 is our bestselling catalog number among all CD31/PECAM1 antibodies and it has been cited in two publications, both explaining its use in IHC-P assays (Pratheeshkumar et al. 2012 PLoS One. 2012;7(10):e47516; and Pratheeshkumar et al. 2012 PLoS One. 2012;7(12):e52279)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-71663AF405
Alexa Fluor 488 Labeled NBP1-71663AF488
Alexa Fluor 647 Labeled NBP1-71663AF647
Alexa Fluor 700 Labeled NBP1-71663AF700
Biotin Labeled NBP1-71663B
DyLight 405 Labeled NBP1-71663V
DyLight 488 Labeled NBP1-71663G
DyLight 550 Labeled NBP1-71663R
DyLight 650 Labeled NBP1-71663C
FITC Labeled NBP1-71663F
HRP Labeled NBP1-71663H
PE Labeled NBP1-71663PE

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Bioinformatics Tool for CD31/PECAM-1 Antibody (NBP1-71663)

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Blogs on CD31/PECAM-1.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

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Recent Reviews

5
5
2
4
0
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0
2
0
1
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Anonymous
09/05/2013
Application: WB
Species: Mouse

Anonymous
09/05/2013
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol PECAM1
Entrez
Uniprot