Von Willebrand Factor Antibody

Dual RNAscope ISH-IHC: Von Willebrand Factor Antibody [NB600-586] - Formalin-fixed paraffin-embedded tissue sections of human metastatic tonsil were probed for vWF mRNA (ACD RNAScope probe, catalog # 5860461; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal (Novus catalog # NB600-586) at 1:500 dilution for 1 hour at room temperature followed by incubation with the anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).

Immunohistochemistry: Von Willebrand Factor Antibody [NB600-586] - Formalin fixed paraffin embedded human tonsil stained with Factor VIII antibody.

Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586] - Staining of formalin-fixed, paraffin-embedded Human Kidney sections with 1:2,000 Rabbit Anti-von Willebrand Factor.

Immunohistochemistry-Frozen: Von Willebrand Factor Antibody [NB600-586] - Canine brain/spinal cord. VWF antibody in green. A pericyte marker in red. Alexa Fluor conjugated secondary antibodies were used for visualization. IHC-Fr image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586] - Staining of formalin-fixed, paraffin-embedded Human Kidney sections with 1:2,000 Rabbit Anti-von Willebrand Factor.

• Reactivity: Hu, Mu, Ca, Fe
• Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Dual ISH-IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Von Willebrand Factor Antibody Summary

• Immunogen: Purified antisera of the Von Willebrand Factor (factor VIII R:ag) related antigen in plasma.
• Localization: Secreted. Localized to storage granules.
• Marker: Endothelial Cell Marker
• Specificity: The antibody specifically reacts with the cytoplasm of human endothelial cells from normal and neoplastic blood and from lymphatic vessels. It also reacts with human endocardium, platelets and megakaryocytes. It may be used for immunohistological studies of the vascular structures in frozen tissue preparations and enzymatically pre-digested, formalin, methacarn, Bouin's or ethanol-fixed and paraffin-embedded tissue sections.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VWF
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Dual RNAscope ISH-IHC
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:1000
• Application Notes: IHC-P: We recommend proteolycic antigen retrieval. After deparaffinization treat with 0.1% Pronase in PBS for 5-10 minutes at 37 degrees C. Alternatively, digest with 0.1% Trypsin in PBS for 25 minutes at 37 degrees C. Gently rinse in PBS. This Von Willebrand Factor Antibody is validated for IHC-Fr from a verified customer review.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31738896). Feline reactivity reported in scientific literature (PMID:31616579).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS (pH 7.4)
• Preservative: 15mM Sodium Azide
• Purity: IgG purified

Alternate Names for Von Willebrand Factor Antibody

• coagulation factor VIII VWF
• F8
• F8VWF
• von Willebrand factor
• VWD
• vWF

Background

Human Von Willebrand Factor(factor VIII R:Ag) is a 270 kDa multimeric plasma gylcoprotein. It mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation factor VIII. The von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. The factor is synthesized by endothelial cells and is also present in platelets and megakaryocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Von Willebrand Factor Antibody (NB600-586)(7)

Publications using NB600-586

• Mary T, Kevin S, Kazuya O et al. Development and validation of methods to visualize conventional aqueous outflow pathways in canine primary angle closure glaucoma Veterinary Ophthalmology 2021-09-28 [PMID: 34581493] (ICC/IF, Canine)
• Xu X, Wang D, Han Z et al. A novel rat model of chronic subdural hematoma: Induction of inflammation and angiogenesis in the subdural space mimicking human-like features of progressively expanding hematoma Brain research bulletin 2021-04-28 [PMID: 33932488]
• Garrido-Gomez T, QuiNonero A, Dominguez F et al. Preeclampsia: a defect in decidualization is associated with deficiency of Annexin A2 Am. J. Obstet. Gynecol. 2019-11-15 [PMID: 31738896] (IF/IHC, Mouse)
• Snyder K, Oikawa K, Williams J, et al. Imaging Distal Aqueous Outflow Pathways in a Spontaneous Model of Congenital Glaucoma. Transl Vis Sci Technol. 2019-10-09 [PMID: 31616579] (IF, Feline)
• Ghanem LY, Mansour IM, Abulata N et al. Liver Macrophage Depletion Ameliorates The Efect of Mesenchymal Stem Cell Transplantation in a Murine Model of Injured Liver Scientific Reports 2019-01-10 [PMID: 30631109] (IF/IHC, Mouse)
• Shao X, Ren H, Wang YL et al. Targeting antitumor effect of rhTNF-alpha fusion protein mediated by matrix metalloproteinase-2. Oncol. Rep. 2015-02-01 [PMID: 25421954]
• Hu X, Neoh KG, Zhang J et al. Immobilization strategy for optimizing VEGF's concurrent bioactivity towards endothelial cells and osteoblasts on implant surfaces Biomaterials 2012-11-01 [PMID: 22884814] (ICC/IF, Human)

Reviews for Von Willebrand Factor Antibody (NB600-586) (3)

Average Rating: 4.3

(Based on 3 reviews)

We have 3 reviews tested in 3 species: Mouse, Canine, Feline.

reviewed by: Kazuya Oikawa IHC-Fr Canine 12/16/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: brain and spinal cord
• Species: Canine
• Lot: 069M4884V

Comments

• Comments: Alexa Fluor conjugated secondary antibodies were used.

reviewed by: Verified Customer IF Mouse 01/05/2018

Summary

• Application: Immunofluorescence
• Sample Tested: Endothelial
• Species: Mouse
• Lot: 126M4801V

reviewed by: Kazuya Oikawa IHC-Fr Feline 08/01/2017

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Connective tissue
• Species: Feline
• Lot: 046M4770V

FAQs for Von Willebrand Factor Antibody (NB600-586). (Showing 1 - 1 of 1 FAQs).

1. I have Von Willebrand Factor Antibody (NB600-586) being stored at -20 degrees Celsius and was wondering what the proper way to thaw them prior to use?
   • There is no special method. You can just take it out of the freezer and let it thaw on the lab bench. We suggest you could check it in an hour; it's not a large volume so shouldn't take very long.

Bioinformatics

• Gene Symbol: VWF
• Entrez:
  • 7450(Human)
• Uniprot:
  • P04275()