Von Willebrand Factor Antibody

Images

 
Orthogonal Strategies: Dual RNAscope ISH-IHC: Von Willebrand Factor Antibody [NB600-586] - Formalin-fixed paraffin-embedded tissue sections of human metastatic tonsil were probed for vWF mRNA (ACD RNAScope probe, ...read more
Immunohistochemistry: Von Willebrand Factor Antibody [NB600-586] - Formalin fixed paraffin embedded human tonsil stained with Factor VIII antibody.
Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586] - Staining of formalin-fixed, paraffin-embedded Human Kidney sections with 1:2,000 Rabbit Anti-von Willebrand Factor.
Immunocytochemistry/ Immunofluorescence: Von Willebrand Factor Antibody [NB600-586]
Immunohistochemistry-Frozen: Von Willebrand Factor Antibody [NB600-586] - Canine brain/spinal cord. VWF antibody in green. A pericyte marker in red. Alexa Fluor conjugated secondary antibodies were used for ...read more
Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586] - Staining of formalin-fixed, paraffin-embedded Human Kidney sections with 1:2,000 Rabbit Anti-von Willebrand Factor.

Product Details

Summary
Reactivity Hu, Mu, Ca, FeSpecies Glossary
Applications ICC/IF, IHC, IHC-Fr, IHC-P, Dual ISH-IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Von Willebrand Factor Antibody Summary

Immunogen
Purified antisera of the Von Willebrand Factor (factor VIII R:ag) related antigen in plasma.
Localization
Secreted. Localized to storage granules.
Marker
Endothelial Cell Marker
Specificity
The antibody specifically reacts with the cytoplasm of human endothelial cells from normal and neoplastic blood and from lymphatic vessels. It also reacts with human endocardium, platelets and megakaryocytes. It may be used for immunohistological studies of the vascular structures in frozen tissue preparations and enzymatically pre-digested, formalin, methacarn, Bouin's or ethanol-fixed and paraffin-embedded tissue sections.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VWF
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:1000
  • Dual RNAscope ISH-IHC
Application Notes
This Von Willebrand Factor Antibody is validated for IHC-Fr from a verified customer review.
Reviewed Applications
Read 3 Reviews rated 4.3
using
NB600-586 in the following applications:

Publications
Read Publications using
NB600-586 in the following applications:

  • 1 publication
  • IF
    1 publication
  • IHC
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31738896). Feline reactivity reported in scientific literature (PMID:31616579).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10 mM PBS (pH 7.4)
Preservative
15mM Sodium Azide
Purity
IgG purified

Alternate Names for Von Willebrand Factor Antibody

  • coagulation factor VIII VWF
  • F8
  • F8VWF
  • von Willebrand factor
  • VWD
  • vWF

Background

Human Von Willebrand Factor(factor VIII R:Ag) is a 270 kDa multimeric plasma gylcoprotein. It mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation factor VIII. The von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. The factor is synthesized by endothelial cells and is also present in platelets and megakaryocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Von Willebrand Factor Antibody (NB600-586)(4)

Reviews for Von Willebrand Factor Antibody (NB600-586) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 3 species: Mouse, Canine, Feline.

Reviews using NB600-586:
Filter by Applications
IHC-Fr
(2)
IF
(1)
All Applications
Filter by Species
Mouse
(1)
Canine
(1)
Feline
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Von Willebrand Factor NB600-586
Enlarge
reviewed by:
Anonymous
IHC-Fr Canine 12/16/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Tested brain and spinal cord
SpeciesCanine
Lot069M4884V

Comments

CommentsAlexa Fluor conjugated secondary antibodies were used.
Immunofluorescence Von Willebrand Factor NB600-586
Enlarge
reviewed by:
Yukio Sasaki
IF Mouse 01/05/2018
View

Summary

ApplicationImmunofluorescence
Sample TestedEndothelial
SpeciesMouse
Lot126M4801V
Immunohistochemistry-Frozen Von Willebrand Factor NB600-586
Enlarge
reviewed by:
Anonymous
IHC-Fr Feline 08/01/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedConnective tissue
SpeciesFeline
Lot046M4770V

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Von Willebrand Factor Antibody (NB600-586). (Showing 1 - 1 of 1 FAQs).

  1. I have Von Willebrand Factor Antibody (NB600-586) being stored at -20 degrees Celsius and was wondering what the proper way to thaw them prior to use?
    • There is no special method. You can just take it out of the freezer and let it thaw on the lab bench. We suggest you could check it in an hour; it's not a large volume so shouldn't take very long.

Secondary Antibodies

 

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Thrombomodulin - A multifunctional protein with roles in inflammation and coagulation
Thrombomodulin, also known as BDCA-3, is a glycosylated transmembrane protein present on the surface of vascular endothelial cells. Thrombomodulin is a high-affinity receptor for thrombin, a key protein in the coagulation cascade. Formation of the...  Read full blog post.

Von Willebrand Factor: An important mediator and carrier of hemostasis
Human Von Willebrand Factor (factor VIII R: Ag) is a 270 kDa multimeric plasma gylcoprotein. Important in the maintenance of hemostasis, it mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation facto...  Read full blog post.

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Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
0

Anonymous
12/16/2019
Application: IHC-Fr
Species: Canine

Yukio Sasaki
01/05/2018
Application: IF
Species: Mouse

Anonymous
08/01/2017
Application: IHC-Fr
Species: Feline

Bioinformatics

Gene Symbol VWF
Entrez
Uniprot