VEGFR2/KDR/Flk-1 Antibody [Unconjugated]

VEGFR2/KDR/Flk‑1 was detected in immersion fixed frozen sections of mouse embryo (14 d.p.c.) using 15 µg/mL Goat Anti-Mouse VEGFR2/KDR/Flk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF644) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to mesenchymal cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

VEGFR2 was detected in acetone fixed cryosections of mouse kidney tissue using Goat Anti-Mouse VEGFR2/KDR/Flk-1 Polyclonal Antibody (Catalog # AF644) for 50 minutes at room temperature. Tissues were stained with rabbit anti-goat secondaryantibody and HRP polymer-conjugated anti-rabbit IgG followed by AEC+Substrate Chromogen (red) followed by counterstaining with hematoxylin (blue). Experiments were carried out and the image was provided by Dr. Grietje Molema, University Medical Center Groningen, The Netherlands.

Recombinant Mouse VEGFR2/KDR/Flk‑1 Fc Chimera (Catalog # 443-KD) inhibits Recombinant Mouse VEGF164 (Catalog # 493-MV) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse VEGF164 (5 ng/mL) activity elicited by Recombinant Mouse VEGFR2/KDR/Flk‑1 Fc Chimera (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse VEGFR2/KDR/Flk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF644). The ND50 is typically 0.1‑0.3 µg/mL.

• Reactivity: Mu
• Applications: WB, Flow, IHC, B/N, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

VEGFR2/KDR/Flk-1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse VEGFR2
  Ala20-Glu762
  Accession # P35918
• Specificity: Detects mouse VEGFR2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human VEGFR2 is observed and less than 2% cross-reactivity with recombinant mouse (rm) VEGFR1 and rmVEGFR3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KDR
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.1-0.3 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR2/KDR/Flk-1 Antibody [Unconjugated]

• CD309 antigen
• CD309
• EC 2.7.10
• EC 2.7.10.1
• Fetal liver kinase 1
• fetal liver kinase-1
• Flk1
• Flk-1
• FLK1tyrosine kinase growth factor receptor
• KDR
• kinase insert domain receptor (a type III receptor tyrosine kinase)
• Kinase insert domain receptor
• KRD1
• Ly73
• Protein-tyrosine kinase receptor flk-1
• soluble VEGFR2
• vascular endothelial growth factor receptor 2
• VEGF R2
• VEGFR
• VEGFR2
• VEGFR-2

Background

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1), and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.

Mouse VEGFR2 cDNA encodes a 1367 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGFR2 is composed of a 743 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 583 aa residue cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
2. Achen, M.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VEGFR2/KDR/Flk-1 Antibody (AF644)(55)

Publications using AF644

• F Mueller-Di, W Lederle, A Rix, S Koletnik, D Doleschel, M Snelting, F Gremse, F Kiessling Molecular Ultrasound Imaging Depicts the Modulation of Tumor Angiogenesis by Acetylsalicylic Acid International Journal of Molecular Sciences, 2023-04-11;24(8):. 2023-04-11 [PMID: 37108226] (IHC, Mouse)
• E Lee, EA Lee, E Kong, H Chon, M Llaiqui-Co, CH Park, BY Park, NR Kang, JS Yoo, HS Lee, HS Kim, SH Park, SW Choi, D Vestweber, JH Lee, P Kim, WS Lee, I Kim An agonistic anti-Tie2 antibody suppresses the normal-to-tumor vascular transition in the glioblastoma invasion zone Experimental & Molecular Medicine, 2023-02-24;55(2):470-484. 2023-02-24 [PMID: 36828931] (IHC, Mouse)
• JM Arpino, H Yin, EK Prescott, SCR Staples, Z Nong, F Li, J Chevalier, B Balint, C O'Neil, R Mortuza, S Milkovich, JJ Lee, D Lorusso, M Sandig, DW Hamilton, DW Holdsworth, TL Poepping, CG Ellis, JG Pickering Low-flow intussusception and metastable VEGFR2 signaling launch angiogenesis in ischemic muscle Science Advances, 2021-11-26;7(48):eabg9509. 2021-11-26 [PMID: 34826235] (IHC, Mouse)
• F Progatzky, M Shapiro, SH Chng, B Garcia-Cas, CH Classon, S Sevgi, A Laddach, AC Bon-Frauch, R Lasrado, M Rahim, EM Amaniti, S Boeing, K Shah, LJ Entwistle, A Suárez-Bon, MS Wilson, B Stockinger, V Pachnis Regulation of intestinal immunity and tissue repair by enteric glia Nature, 2021-10-20;599(7883):125-130. 2021-10-20 [PMID: 34671159] (IHC, Mouse)
• DH Yeom, YS Lee, I Ryu, S Lee, B Sung, HB Lee, D Kim, JH Ahn, E Ha, YS Choi, SH Lee, WK You ABL001, a Bispecific Antibody Targeting VEGF and DLL4, with Chemotherapy, Synergistically Inhibits Tumor Progression in Xenograft Models International Journal of Molecular Sciences, 2020-12-29;22(1):. 2020-12-29 [PMID: 33383646] (IHC, Mouse)
• SP Hong, MJ Yang, H Cho, I Park, H Bae, K Choe, SH Suh, RH Adams, K Alitalo, D Lim, GY Koh Distinct fibroblast subsets regulate lacteal integrity through YAP/TAZ-induced VEGF-C in intestinal villi Nat Commun, 2020-08-14;11(1):4102. 2020-08-14 [PMID: 32796823] (IHC, Mouse)
• L Su, X Lei, H Ma, C Feng, J Jiang, J Jiao PRDM16 orchestrates angiogenesis via neural differentiation in the developing brain Cell Death Differ., 2020-02-03;0(0):. 2020-02-03 [PMID: 32015502] (IHC, Mouse)
• E Harde, L Nicholson, B Furones Cu, D Bissen, S Wigge, S Urban, M Segarra, C Ruiz de Al, A Acker-Palm EphrinB2 regulates VEGFR2 during dendritogenesis and hippocampal circuitry development Elife, 2019-12-23;8(0):. 2019-12-23 [PMID: 31868584] (IHC, Mouse)
• D Duc, S Vigne, J Bernier-La, Y Yersin, F Ruiz, N Gaïa, S Leo, V Lazarevic, J Schrenzel, TV Petrova, C Pot Disrupting Myelin-Specific Th17 Cell Gut Homing Confers Protection in an Adoptive Transfer Experimental Autoimmune Encephalomyelitis Cell Rep, 2019-10-08;29(2):378-390.e4. 2019-10-08 [PMID: 31597098] (IHC, Mouse)
• S Hendrikx, S Coso, B Prat-Luri, L Wetterwald, A Sabine, CA Franco, S Nassiri, N Zangger, H Gerhardt, M Delorenzi, TV Petrova Endothelial Calcineurin Signaling Restrains Metastatic Outgrowth by Regulating Bmp2 Cell Rep, 2019-01-29;26(5):1227-1241.e6. 2019-01-29 [PMID: 30699351] (IHC, Mouse)
• C Choquet, THM Nguyen, P Sicard, E Buttigieg, TT Tran, F Kober, I Varlet, R Sturny, MW Costa, RP Harvey, C Nguyen, P Rihet, S Richard, M Bernard, RG Kelly, N Lalevée, L Miquerol Deletion of Nkx2-5 in trabecular myocardium reveals the developmental origins of pathological heterogeneity associated with ventricular non-compaction cardiomyopathy PLoS Genet., 2018-07-06;14(7):e1007502. 2018-07-06 [PMID: 29979676] (IHC, Mouse)
• JM Rosin, DM Kurrasch In utero electroporation induces cell death and alters embryonic microglia morphology and expression signatures in the developing hypothalamus J Neuroinflammation, 2018-06-12;15(1):181. 2018-06-12 [PMID: 29895301] (IHC-Fr, Mouse)
• IK Kim, K Kim, E Lee, DS Oh, CS Park, S Park, JM Yang, JH Kim, HS Kim, DT Shima, JH Kim, SH Hong, YH Cho, YH Kim, JB Park, GY Koh, YS Ju, HK Lee, S Lee, I Kim Sox7 promotes high-grade glioma by increasing VEGFR2-mediated vascular abnormality J. Exp. Med., 2018-02-14;0(0):. 2018-02-14 [PMID: 29444818] (IHC-P, Mouse)
• S Rhee, JI Chung, DA King, G D'amato, DT Paik, A Duan, A Chang, D Nagelberg, B Sharma, Y Jeong, M Diehn, JC Wu, AJ Morrison, K Red-Horse Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease Nat Commun, 2018-01-25;9(1):368. 2018-01-25 [PMID: 29371594] (IHC, Mouse)
• CJ Drummond, JA Hanna, MR Garcia, DJ Devine, AJ Heyrana, D Finkelstei, JE Rehg, ME Hatley Hedgehog Pathway Drives Fusion-Negative Rhabdomyosarcoma Initiated From Non-myogenic Endothelial Progenitors Cancer Cell, 2018-01-08;33(1):108-124.e5. 2018-01-08 [PMID: 29316425] (IHC, Mouse)
• JL Sones, AA Merriam, A Seffens, DA Brown-Gran, SD Butler, AM Zhao, X Xu, CJ Shawber, JK Grenier, NC Douglas Angiogenic factor imbalance precedes complement deposition in placentae of the BPH/5 model of preeclampsia FASEB J., 2018-01-08;0(0):. 2018-01-08 [PMID: 29279353] (IHC, Mouse)
• Y Chen, H Xu, G Lin Generation of iPSC-derived limb progenitor-like cells for stimulating phalange regeneration in the adult mouse Cell Discov, 2017-12-19;3(0):17046. 2017-12-19 [PMID: 29263795] (IHC, Mouse)
• J Kim, DY Park, H Bae, DY Park, D Kim, CK Lee, S Song, TY Chung, DH Lim, Y Kubota, YK Hong, Y He, HG Augustin, G Oliver, GY Koh Impaired angiopoietin/Tie2 signaling compromises Schlemm&#039;s canal integrity and induces glaucoma J. Clin. Invest., 2017-09-18;0(0):. 2017-09-18 [PMID: 28920924] (IHC, Mouse)
• H Wu, J Zhao, B Fu, S Yin, C Song, J Zhang, S Zhao, Y Zhang Retinoic acid-induced upregulation of miR-219 promotes the differentiation of embryonic stem cells into neural cells Cell Death Dis, 2017-07-27;8(7):e2953. 2017-07-27 [PMID: 28749472] (Western Blot, Mouse)
• K Heinolaine, S Karaman, G D'Amico, T Tammela, R Sormunen, L Eklund, K Alitalo, G Zarkada VEGFR3 Modulates Vascular Permeability by Controlling VEGF/VEGFR2 Signaling Circ. Res, 2017-03-15;0(0):. 2017-03-15 [PMID: 28298294] (Western Blot, Mouse)
• UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams Cell-matrix signals specify bone endothelial cells during developmental osteogenesis Nat. Cell Biol, 2017-02-20;19(3):189-201. 2017-02-20 [PMID: 28218908] (IHC, Mouse)
• MH Ulvmar, I Martinez-C, L Stanczuk, T Mäkinen Pdgfrb-Cre targets lymphatic endothelial cells of both venous and non-venous origins Genesis, 2016-04-21;0(0):. 2016-04-21 [PMID: 27060598] (IHC, Mouse)
• Plein A, Calmont A, Fantin A, Denti L, Anderson N, Scambler P, Ruhrberg C Neural crest-derived SEMA3C activates endothelial NRP1 for cardiac outflow tract septation. J Clin Invest, 2015-06-08;125(7):2661-76. 2015-06-08 [PMID: 26053665] (IHC, Mouse)
• Rama N, Dubrac A, Mathivet T, Ni Charthaigh R, Genet G, Cristofaro B, Pibouin-Fragner L, Ma L, Eichmann A, Chedotal A Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization. Nat Med, 2015-04-20;21(5):483-91. 2015-04-20 [PMID: 25894826] (IHC, Mouse)
• Hou , Sheng Ta, Nilchi , Ladan, Li , Xuesheng, Gangaraju , Sandhya, Jiang , Susan X, Aylsworth , Amy, Monette , Robert, Slinn , Jacqueli Semaphorin3A elevates vascular permeability and contributes to cerebral ischemia-induced brain damage. Sci Rep, 2015-01-20;5(0):7890. 2015-01-20 [PMID: 25601765] (Bioassay, Rat)
• Aspelund A, Tammela T, Antila S, Nurmi H, Leppanen V, Zarkada G, Stanczuk L, Francois M, Makinen T, Saharinen P, Immonen I, Alitalo K The Schlemm&#039;s canal is a VEGF-C/VEGFR-3-responsive lymphatic-like vessel. J Clin Invest, 2014-07-25;124(9):3975-86. 2014-07-25 [PMID: 25061878] (IHC-Fr, Mouse)
• Song K, Herzog B, Sheng M, Fu J, McDaniel J, Chen H, Ruan J, Xia L Lenalidomide inhibits lymphangiogenesis in preclinical models of mantle cell lymphoma. Cancer Res, 2013-10-24;73(24):7254-64. 2013-10-24 [PMID: 24158094] (Western Blot, Human)
• Goldman O, Han S, Sourisseau M, Dziedzic N, Hamou W, Corneo B, D'Souza S, Sato T, Kotton D, Bissig K, Kalir T, Jacobs A, Evans T, Evans M, Gouon-Evans V KDR identifies a conserved human and murine hepatic progenitor and instructs early liver development. Cell Stem Cell, 2013-06-06;12(6):748-60. 2013-06-06 [PMID: 23746980] (IHC-Fr, Mouse)
• Carmi Y, Dotan S, Rider P, Kaplanov I, White M, Baron R, Abutbul S, Huszar M, Dinarello C, Apte R, Voronov E The role of IL-1beta in the early tumor cell-induced angiogenic response. J Immunol, 2013-03-08;190(7):3500-9. 2013-03-08 [PMID: 23475218] (IHC-Fr, Mouse)
• Fantin A, Vieira J, Plein A, Maden C, Ruhrberg C The embryonic mouse hindbrain as a qualitative and quantitative model for studying the molecular and cellular mechanisms of angiogenesis. Nat Protoc, 2013-02-01;8(2):418-29. 2013-02-01 [PMID: 23424750] (IHC, Mouse)
• Sun Z, Li X, Massena S, Kutschera S, Padhan N, Gualandi L, Sundvold-Gjerstad V, Gustafsson K, Choy WW, Zang G, Quach M, Jansson L, Phillipson M, Abid MR, Spurkland A, Claesson-Welsh L VEGFR2 induces c-Src signaling and vascular permeability in vivo via the adaptor protein TSAd. J. Exp. Med., 2012-06-11;209(7):1363-77. 2012-06-11 [PMID: 22689825] (Western Blot, Mouse)
• Benedito R, Rocha S, Woeste M, Zamykal M, Radtke F, Casanovas O, Duarte A, Pytowski B, Adams R Notch-dependent VEGFR3 upregulation allows angiogenesis without VEGF-VEGFR2 signalling. Nature, 2012-03-18;484(7392):110-4. 2012-03-18 [PMID: 22426001] (ELISA Development, Mouse)
• Schreurs MP, Houston EM, May V, Cipolla MJ The adaptation of the blood-brain barrier to vascular endothelial growth factor and placental growth factor during pregnancy. FASEB J., 2011-09-12;26(1):355-62. 2011-09-12 [PMID: 21911594] (Neutralization, Rat)
• Efe JA, Hilcove S, Kim J, Zhou H, Ouyang K, Wang G, Chen J, Ding S Conversion of mouse fibroblasts into cardiomyocytes using a direct reprogramming strategy. Nat. Cell Biol., 2011-01-30;13(3):215-22. 2011-01-30 [PMID: 21278734] (ICC, Mouse)
• Sawamiphak S, Seidel S, Essmann CL, Wilkinson GA, Pitulescu ME, Acker T, Acker-Palmer A Ephrin-B2 regulates VEGFR2 function in developmental and tumour angiogenesis. Nature, 2010-05-05;465(7297):487-91. 2010-05-05 [PMID: 20445540] (ICC, Immunoprecipitation, Mouse)
• Lohela M, Helotera H, Haiko P, Dumont DJ, Alitalo K Transgenic induction of vascular endothelial growth factor-C is strongly angiogenic in mouse embryos but leads to persistent lymphatic hyperplasia in adult tissues. Am. J. Pathol., 2008-11-06;173(6):1891-901. 2008-11-06 [PMID: 18988807] (IHC, Mouse)
• Tammela T, Zarkada G, Wallgard E, Murtomaki A, Suchting S, Wirzenius M, Waltari M, Hellstrom M, Schomber T, Peltonen R, Freitas C, Duarte A, Isoniemi H, Laakkonen P, Christofori G, Yla-Herttuala S, Shibuya M, Pytowski B, Eichmann A, Betsholtz C, Alitalo K Blocking VEGFR-3 suppresses angiogenic sprouting and vascular network formation. Nature, 2008-06-25;454(7204):656-60. 2008-06-25 [PMID: 18594512] (IHC-Fr, Mouse)
• Fernandez L, Rodriguez S, Huang H, Chora A, Fernandes J, Mumaw C, Cruz E, Pollok K, Cristina F, Price JE, Ferkowicz MJ, Scadden DT, Clauss M, Cardoso AA, Carlesso N Tumor necrosis factor-alpha and endothelial cells modulate Notch signaling in the bone marrow microenvironment during inflammation. Exp. Hematol., 2008-05-01;36(5):545-558. 2008-05-01 [PMID: 18439488] (IHC-P, Mouse)
• Dombrowski SM, Deshpande A, Dingwall C, Leichliter A, Leibson Z, Luciano MG Chronic hydrocephalus-induced hypoxia: increased expression of VEGFR-2+ and blood vessel density in hippocampus. Neuroscience, 2007-12-14;152(2):346-59. 2007-12-14 [PMID: 18262364] (IHC-Fr, Canine)
• Depeille P, Young JJ, Boguslawski EA, Berghuis BD, Kort EJ, Resau JH, Frankel AE, Duesbery NS Anthrax lethal toxin inhibits growth of and vascular endothelial growth factor release from endothelial cells expressing the human herpes virus 8 viral G protein coupled receptor. Clin. Cancer Res., 2007-10-01;13(19):5926-34. 2007-10-01 [PMID: 17908989] (ICC, Mouse)
• Li X, Edholm D, Lanner F, Breier G, Farnebo F, Dimberg A, Claesson-Welsh L Lentiviral rescue of vascular endothelial growth factor receptor-2 expression in flk1-/- embryonic stem cells shows early priming of endothelial precursors. Stem Cells, 2007-08-16;25(12):2987-95. 2007-08-16 [PMID: 17702981] (ICC, Immunoprecipitation, Mouse)
• Isayeva T, Chanda D, Kallman L, Eltoum IE, Ponnazhagan S Effects of sustained antiangiogenic therapy in multistage prostate cancer in TRAMP model. Cancer Res., 2007-06-15;67(12):5789-97. 2007-06-15 [PMID: 17575146] (IHC-P, Mouse)
• Wirzenius M, Tammela T, Uutela M, He Y, Odorisio T, Zambruno G, Nagy JA, Dvorak HF, Yla-Herttuala S, Shibuya M, Alitalo K Distinct vascular endothelial growth factor signals for lymphatic vessel enlargement and sprouting. J. Exp. Med., 2007-05-29;204(6):1431-40. 2007-05-29 [PMID: 17535974] (IHC, Mouse)
• Mohamedali KA, Poblenz AT, Sikes CR, Navone NM, Thorpe PE, Darnay BG, Rosenblum MG Inhibition of prostate tumor growth and bone remodeling by the vascular targeting agent VEGF121/rGel. Cancer Res., 2006-11-15;66(22):10919-28. 2006-11-15 [PMID: 17108129] (Neutralization, Mouse)
• Yamaji-Kegan K, Su Q, Angelini DJ, Champion HC, Johns RA Hypoxia-induced mitogenic factor has proangiogenic and proinflammatory effects in the lung via VEGF and VEGF receptor-2. Am. J. Physiol. Lung Cell Mol. Physiol., 2006-08-04;291(6):L1159-68. 2006-08-04 [PMID: 16891392] (Neutralization, Mouse)
• Getmanova EV, Chen Y, Bloom L, Gokemeijer J, Shamah S, Warikoo V, Wang J, Ling V, Sun L Antagonists to human and mouse vascular endothelial growth factor receptor 2 generated by directed protein evolution in vitro. Chem. Biol., 2006-05-01;13(5):549-56. 2006-05-01 [PMID: 16720276] (Flow Cytometry, Human)
• Schmitz V, Vilanueva H, Raskopf E, Hilbert T, Barajas M, Dzienisowicz C, Gorschluter M, Strehl J, Rabe C, Sauerbruch T, Prieto J, Caselmann WH, Qian C Increased VEGF levels induced by anti-VEGF treatment are independent of tumor burden in colorectal carcinomas in mice. Gene Ther., 2006-04-13;13(16):1198-205. 2006-04-13 [PMID: 16617302] (Western Blot, Mouse)
• Nozaki M, Sakurai E, Raisler BJ, Baffi JZ, Witta J, Ogura Y, Brekken RA, Sage EH, Ambati BK, Ambati J Loss of SPARC-mediated VEGFR-1 suppression after injury reveals a novel antiangiogenic activity of VEGF-A. J. Clin. Invest., 2006-02-01;116(2):422-9. 2006-02-01 [PMID: 16453023] (Immunoprecipitation, Neutralization, Mouse)
• Gudehithlu KP, Ahmed N, Wu H, Litbarg NO, Garber SL, Arruda JA, Dunea G, Singh AK Antagonism of vascular endothelial growth factor results in microvessel attrition and disorganization of wound tissue. J. Lab. Clin. Med., 2005-04-01;145(4):194-203. 2005-04-01 [PMID: 15962838] (IHC-P, Rat)
• Raskopf E, Dzienisowicz C, Hilbert T, Rabe C, Leifeld L, Wernert N, Sauerbruch T, Prieto J, Qian C, Caselmann WH, Schmitz V Effective angiostatic treatment in a murine metastatic and orthotopic hepatoma model. Hepatology, 2005-06-01;41(6):1233-40. 2005-06-01 [PMID: 15915456] (ELISA Development, Western Blot, Mouse)
• Dikov MM, Ohm JE, Ray N, Tchekneva EE, Burlison J, Moghanaki D, Nadaf S, Carbone DP Differential roles of vascular endothelial growth factor receptors 1 and 2 in dendritic cell differentiation. J. Immunol., 2005-01-01;174(1):215-22. 2005-01-01 [PMID: 15611243] (Western Blot, Mouse)
• Bairey O, Boycov O, Kaganovsky E, Zimra Y, Shaklai M, Rabizadeh E All three receptors for vascular endothelial growth factor (VEGF) are expressed on B-chronic lymphocytic leukemia (CLL) cells. Leuk. Res., 2004-03-01;28(3):243-8. 2004-03-01 [PMID: 14687619] (ICC, Human)
• Gerhardt H, Golding M, Fruttiger M, Ruhrberg C, Lundkvist A, Abramsson A, Jeltsch M, Mitchell C, Alitalo K, Shima D, Betsholtz C VEGF guides angiogenic sprouting utilizing endothelial tip cell filopodia. J. Cell Biol., 2003-06-16;161(6):1163-77. 2003-06-16 [PMID: 12810700] (IHC-Fr, Neutralization, Rat)
• Silvestre JS, Tamarat R, Ebrahimian TG, Le-Roux A, Clergue M, Emmanuel F, Duriez M, Schwartz B, Branellec D, Levy BI Vascular endothelial growth factor-B promotes in vivo angiogenesis. Circ. Res., 2003-06-12;93(2):114-23. 2003-06-12 [PMID: 12805240] (Neutralization, Mouse)
• Kozawa O, Yoshimi N, Akamatsu S, Hara A, Mori H, Uematsu T Lack of alpha2-antiplasmin promotes pulmonary heart failure via overrelease of VEGF after acute myocardial infarction. Blood, 2002-10-01;100(7):2487-93. 2002-10-01 [PMID: 12239160] (Neutralization, Mouse)

Reviews for VEGFR2/KDR/Flk-1 Antibody (AF644) (3)

Average Rating: 4.3

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Mouse.

reviewed by: Bhavin Shah Immunofluorescence -Frozen tissue sections Mouse 04/28/2020

Summary

• Application: Other
• Sample Tested: Brain,Embryonic brain
• Species: Mouse
• Lot: COA0412081

Comments

• Comments: The cryo frozen samples were blocked with 10% NDS + 0.5% triton-x100 in PBS for 2 hours before using Primary antibody ( in the same blocking buffer) at 4 degrees overnight. Secondary AF488 was used for 2h at RT

reviewed by: Verified Customer IHC-Fr Mouse 01/09/2015

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: See PMID 23746980
• Species: Mouse

reviewed by: Verified Customer IF Mouse 01/09/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23746980
• Species: Mouse

Bioinformatics

• Gene Symbol: KDR
• Uniprot:
  • P35918()