VEGFR2/KDR/Flk-1 Antibody [Unconjugated]

Images

 
Recombinant Mouse VEGFR2/KDR/Flk‑1 Fc Chimera (Catalog # 443-KD) inhibits Recombinant Mouse VEGF164 (Catalog # 493-MV) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner ...read more
VEGFR2 was detected in acetone fixed cryosections of mouse kidney tissue using Goat Anti-Mouse VEGFR2/KDR/Flk-1 Polyclonal Antibody (Catalog # AF644) for 50 minutes at room temperature. Tissues were stained with rabbit ...read more
VEGFR2/KDR/Flk‑1 was detected in immersion fixed frozen sections of mouse embryo (14 d.p.c.) using 15 µg/mL Goat Anti-Mouse VEGFR2/KDR/Flk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF644) overnight ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, B/N, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

VEGFR2/KDR/Flk-1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse VEGFR2
Ala20-Glu762
Accession # P35918
Specificity
Detects mouse VEGFR2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human VEGFR2 is observed and less than 2% cross-reactivity with recombinant mouse (rm) VEGFR1 and rmVEGFR3 is observed.
Details of Functionality
ActivityAssaywCitation not found None None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KDR
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
  • Neutralization 0.1-0.3 ug/mL
Reviewed Applications
Read 3 Reviews rated 4.3
using
AF644 in the following applications:

Publications
Read Publications using
AF644 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR2/KDR/Flk-1 Antibody [Unconjugated]

  • CD309 antigen
  • CD309
  • EC 2.7.10
  • EC 2.7.10.1
  • Fetal liver kinase 1
  • fetal liver kinase-1
  • Flk1
  • Flk-1
  • FLK1tyrosine kinase growth factor receptor
  • KDR
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • Kinase insert domain receptor
  • KRD1
  • Ly73
  • Protein-tyrosine kinase receptor flk-1
  • soluble VEGFR2
  • vascular endothelial growth factor receptor 2
  • VEGF R2
  • VEGFR
  • VEGFR2
  • VEGFR-2

Background

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1), and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.

Mouse VEGFR2 cDNA encodes a 1367 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGFR2 is composed of a 743 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 583 aa residue cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
  2. Achen, M.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VEGFR2/KDR/Flk-1 Antibody (AF644)(48)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 10 applications: Bioassay, ELISA Development, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
Bioassay
(1)
ELISA Development
(2)
Flow Cytometry
(1)
ICC
(3)
IHC
(16)
IHC-Fr
(5)
IHC-P
(4)
Immunoprecipitation
(3)
Neutralization
(6)
Western Blot
(6)
All Applications
Filter By Species
Human
(3)
Mouse
(39)
Rat
(4)
Canine
(1)
All Species
Showing Publications 1 - 10 of 48. Show All 48 Publications.
Publications using AF644 Applications Species
E Harde, L Nicholson, B Furones Cu, D Bissen, S Wigge, S Urban, M Segarra, C Ruiz de Al, A Acker-Palm EphrinB2 regulates VEGFR2 during dendritogenesis and hippocampal circuitry development Elife, 2019;8(0):. 2019 [PMID: 31868584] (IHC, Mouse) IHC Mouse
D Duc, S Vigne, J Bernier-La, Y Yersin, F Ruiz, N Gaïa, S Leo, V Lazarevic, J Schrenzel, TV Petrova, C Pot Disrupting Myelin-Specific Th17 Cell Gut Homing Confers Protection in an Adoptive Transfer Experimental Autoimmune Encephalomyelitis Cell Rep, 2019;29(2):378-390.e4. 2019 [PMID: 31597098] (IHC, Mouse) IHC Mouse
S Hendrikx, S Coso, B Prat-Luri, L Wetterwald, A Sabine, CA Franco, S Nassiri, N Zangger, H Gerhardt, M Delorenzi, TV Petrova Endothelial Calcineurin Signaling Restrains Metastatic Outgrowth by Regulating Bmp2 Cell Rep, 2019;26(5):1227-1241.e6. 2019 [PMID: 30699351] (IHC, Mouse) IHC Mouse
C Choquet, THM Nguyen, P Sicard, E Buttigieg, TT Tran, F Kober, I Varlet, R Sturny, MW Costa, RP Harvey, C Nguyen, P Rihet, S Richard, M Bernard, RG Kelly, N Lalevée, L Miquerol Deletion of Nkx2-5 in trabecular myocardium reveals the developmental origins of pathological heterogeneity associated with ventricular non-compaction cardiomyopathy PLoS Genet., 2018;14(7):e1007502. 2018 [PMID: 29979676] (IHC, Mouse) IHC Mouse
JM Rosin, DM Kurrasch In utero electroporation induces cell death and alters embryonic microglia morphology and expression signatures in the developing hypothalamus J Neuroinflammation, 2018;15(1):181. 2018 [PMID: 29895301] (IHC-Fr, Mouse) IHC-Fr Mouse
IK Kim, K Kim, E Lee, DS Oh, CS Park, S Park, JM Yang, JH Kim, HS Kim, DT Shima, JH Kim, SH Hong, YH Cho, YH Kim, JB Park, GY Koh, YS Ju, HK Lee, S Lee, I Kim Sox7 promotes high-grade glioma by increasing VEGFR2-mediated vascular abnormality J. Exp. Med., 2018;0(0):. 2018 [PMID: 29444818] (IHC-P, Mouse) IHC-P Mouse
S Rhee, JI Chung, DA King, G D'amato, DT Paik, A Duan, A Chang, D Nagelberg, B Sharma, Y Jeong, M Diehn, JC Wu, AJ Morrison, K Red-Horse Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease Nat Commun, 2018;9(1):368. 2018 [PMID: 29371594] (IHC, Mouse) IHC Mouse
CJ Drummond, JA Hanna, MR Garcia, DJ Devine, AJ Heyrana, D Finkelstei, JE Rehg, ME Hatley Hedgehog Pathway Drives Fusion-Negative Rhabdomyosarcoma Initiated From Non-myogenic Endothelial Progenitors Cancer Cell, 2018;33(1):108-124.e5. 2018 [PMID: 29316425] (IHC, Mouse) IHC Mouse
JL Sones, AA Merriam, A Seffens, DA Brown-Gran, SD Butler, AM Zhao, X Xu, CJ Shawber, JK Grenier, NC Douglas Angiogenic factor imbalance precedes complement deposition in placentae of the BPH/5 model of preeclampsia FASEB J., 2017;0(0):. 2017 [PMID: 29279353] (IHC, Mouse) IHC Mouse
Y Chen, H Xu, G Lin Generation of iPSC-derived limb progenitor-like cells for stimulating phalange regeneration in the adult mouse Cell Discov, 2017;3(0):17046. 2017 [PMID: 29263795] (IHC, Mouse) IHC Mouse
Show All 48 Publications.

Reviews for VEGFR2/KDR/Flk-1 Antibody (AF644) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using AF644:
Filter by Applications
IHC-Fr
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
 VEGFR2/KDR/Flk-1 AF644
Enlarge
reviewed by:
Bhavin Shah
Mouse 04/28/2020
View

Summary

Sample TestedBrain,Embryonic brain
SpeciesMouse
LotCOA0412081

Comments

CommentsThe cryo frozen samples were blocked with 10% NDS + 0.5% triton-x100 in PBS for 2 hours before using Primary antibody &#40; in the same blocking buffer&#41; at 4 degrees overnight. Secondary AF488 was used for 2h at RT
 
reviewed by:
Anonymous
IHC-Fr Mouse 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 23746980
SpeciesMouse
 
reviewed by:
Anonymous
IF Mouse 01/09/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23746980
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23746980

Details

Detection NotesSee PMID 23746980

Comments

CommentsPublished: See PMID 23746980Independent review from antibodyresource.com

Product General Protocols

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Video Protocols

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FAQs for VEGFR2/KDR/Flk-1 Antibody (AF644) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF644

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Bioinformatics Tool for VEGFR2/KDR/Flk-1 Antibody (AF644)

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Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
0

Bhavin Shah
04/28/2020
Application:
Species: Mouse

 
Anonymous
01/09/2015
Application: IHC-Fr
Species: Mouse

 
Anonymous
01/09/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol KDR
Uniprot