VE-Cadherin Antibody [Unconjugated]


VE‑Cadherin was detected in immersion fixed paraffin-embedded sections of mouse heart using Goat Anti-Mouse VE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1002) at 3 µg/mL for 1 hour more
Western blot shows lysates of mouse lung tissue and bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse VE‑Cadherin Antigen Affinity-purified Polyclonal Antibody more
Simple Western lane view shows lysates of mouse lung tissue, loaded at 0.2 mg/mL. Specific bands were detected for VE‑Cadherin at approximately 118, 146 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse more

Product Details

Reactivity MuSpecies Glossary
Applications WB, Simple Western, IHC

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VE-Cadherin Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant mouse VE-Cadherin
Accession # 2208309A
Detects mouse VE-Cadherin in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross‑reactivity with recombinant human VE-Cadherin is observed.
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 0.2 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 3-25 ug/mL
Reviewed Applications
Read 3 Reviews rated 4.3
AF1002 in the following applications:

Read Publications using
AF1002 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VE-Cadherin Antibody [Unconjugated]

  • 7B4 antigen
  • 7B4
  • cadherin 5, type 2 (vascular endothelium)
  • cadherin 5, type 2, VE-cadherin (vascular epithelium)
  • Cadherin-5
  • CD144 antigen
  • CD144
  • CDH5
  • endothelial-specific cadherin
  • FLJ17376
  • Vascular endothelial cadherin
  • VECadherin
  • VE-Cadherin


The cadherin (Ca++-dependent adherence) superfamily is a large group of membrane-associated glycoproteins that engage in homotypic, calcium-dependent, cell-cell adhesion events. The superfamily can be divided into at least five major subfamilies based on molecule gene structure, and/or extracellular (EC) and intracellular domains (1-4). Subfamilies include classical/type I, atypical/type II, and desmosomal-related cadherins (1-3). VE-Cadherin (vascular endothelial cadherin; also cadherin-5 and CD144) is a 125 kDa atypical/type II subfamily cadherin. Its subfamily classification is based principally on its genomic structure, as its physical structure is notably divergent from other type II subfamily members (2, 3). Mouse VE-Cadherin is synthesized as a 784 amino acid (aa) type I transmembrane (TM) preproprotein that contains a 24 aa signal peptide, a 21 aa prosequence, a 554 aa extracellular region (ECR), a 21 aa TM segment, and a 164 aa cytoplasmic domain (5, 6). The ECR contains five Ca++-binding cadherin domains that are approximately 105 aa in length. Cadherin domains are comprised of two beta ‑sheets that are oriented like bread in a sandwich. Although complex, the N-terminal cadherin domain mediates trans interactions, while the internal domains contribute to cis multimerizations (7). Mouse VE-Cadherin ECR is 92%, 77%, and 73% aa identical to rat, human and porcine VE-Cadherin ECR, respectively. VE-Cadherin is involved in the maintenance of endothelial permeability. In this regard, VE-Cadherin does not initiate new blood vessel formation; it maintains it once formed. Thus, when VE‑Cadherin is downregulated, cells part and permeability increases (8). Notably, VEGF is known to promote vascular leakage, and apparently does so by inducing a beta ‑arrestin-dependent endocytosis of VE-Cadherin (9). Part of this effect may be mediated by VE‑Cadherin itself which is reported to increase the membrane half-life of VEGF R2 (10). VE-Cadherin acts homotypically at sites of zonula adherens. On each expressing cell, it is proposed that VE-Cadherin first forms a trimer, which then dimerizes with a trimeric counterpart in-trans. Alternatively, two cis-dimers could act in-trans to generate homotypic binding (11). In addition to cell adhesion, VE‑Cadherin also is reported to mediate TGF-beta receptor assembly. When clustered, VE‑Cadherin enhances T beta RII/T beta RI assembly into an active receptor complex on endothelial cells (12). VE-Cadherin is expressed on endothelial cells, trophoblast cells, endothelial progenitor cells and embryonic hematopoietic cells (5, 8, 13, 14).

  1. Patel, S.D. et al. (2007) Curr. Opin. Struct. Biol. 13:690.
  2. Vestweber, D. (2008) Arterioscler. Thromb. Vasc. Biol. 28:223.
  3. Vincent, P.A. et al. (2004) Am. J. Physiol. Cell. Physiol. 286:C987.
  4. Cavallaro, U. et al. (2006) Exp. Cell Res. 312:659.
  5. Breier, G. et al. (1996) Blood 87:630.
  6. Huber, P. et al. (1996) Genomics 32:21.
  7. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
  8. Crosby, C.V. et al. (2005) Blood 105:2771.
  9. Gavard, J. and J.S. Gutkind (2006) Nat. Cell Biol. 8:1223.
  10. Calera, M.R. et al. (2004) Exp. Cell Res. 300:248.
  11. Hewat, E.A. et al. (2007) J. Mol. Biol. 365:744.
  12. Rudini, N. et al. (2008) EMBO J. 27:993.
  13. Kogata, N. et al. (2006) Circ. Res. 98:897.
  14. Ema, M. et al. (2006) Blood 108:4018.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VE-Cadherin Antibody (AF1002)(40)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Western Blot.

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Showing Publications 1 - 10 of 40. Show All 40 Publications.
Publications using AF1002 Applications Species
SJ Jeong, MJ Cho, NY Ko, S Kim, IH Jung, JK Min, SH Lee, JG Park, GT Oh Deficiency of peroxiredoxin 2 exacerbates angiotensin II-induced abdominal aortic aneurysm Exp. Mol. Med., 2020;0(0):. 2020 [PMID: 32929220] (Western Blot, Mouse) Western Blot Mouse
A Tabibian, S Ghaffari, DA Vargas, H Van Ooster, EAV Jones Simulating flow induced migration in vascular remodelling PLoS Comput. Biol., 2020;16(8):e1007874. 2020 [PMID: 32822340] (IHC, Mouse) IHC Mouse
SP Hong, MJ Yang, H Cho, I Park, H Bae, K Choe, SH Suh, RH Adams, K Alitalo, D Lim, GY Koh Distinct fibroblast subsets regulate lacteal integrity through YAP/TAZ-induced VEGF-C in intestinal villi Nat Commun, 2020;11(1):4102. 2020 [PMID: 32796823] (IHC, Mouse) IHC Mouse
Z Wang, F Wang, J Zhong, T Zhu, Y Zheng, T Zhao, Q Xie, F Ma, R Li, Q Tang, F Xu, X Tian, J Zhu Using apelin-based synthetic Notch receptors to detect angiogenesis and treat solid tumors Nat Commun, 2020;11(1):2163. 2020 [PMID: 32358530] (ICC, Mouse) ICC Mouse
KL Betterman, DL Sutton, GA Secker, J Kazenwadel, A Oszmiana, L Lim, N Miura, L Sorokin, BM Hogan, ML Kahn, H McNeill, NL Harvey Atypical cadherin Fat4 orchestrates lymphatic endothelial cell polarity in response to flow J. Clin. Invest., 2020;0(0):. 2020 [PMID: 32182215] (IHC, Mouse) IHC Mouse
JH Wu, YN Li, AQ Chen, CD Hong, CL Zhang, HL Wang, YF Zhou, PC Li, Y Wang, L Mao, YP Xia, QW He, HJ Jin, ZY Yue, B Hu Inhibition of Sema4D/PlexinB1 signaling alleviates vascular dysfunction in diabetic retinopathy EMBO Mol Med, 2020;12(2):e10154. 2020 [PMID: 31943789] (Western Blot, Mouse) Western Blot Mouse
G Chiaverina, L di Blasio, V Monica, M Accardo, M Palmiero, B Peracino, M Vara-Messl, A Puliafito, L Primo Dynamic Interplay between Pericytes and Endothelial Cells during Sprouting Angiogenesis Cells, 2019;8(9):. 2019 [PMID: 31546913] (IHC, Mouse) IHC Mouse
J Zhang, T Supakornde, JR Krambs, M Rao, G Abou-Ezzi, RY Ye, S Li, K Trinkaus, DC Link Bone marrow dendritic cells regulate hematopoietic stem/progenitor cell trafficking J. Clin. Invest., 2019;129(7):2920-2931. 2019 [PMID: 31039135] (IHC, Mouse) IHC Mouse
X Han, Y Wang, W Pu, X Huang, L Qiu, Y Li, W Yu, H Zhao, X Liu, L He, L Zhang, Y Ji, J Lu, KO Lui, B Zhou Lineage Tracing Reveals the Bipotency of SOX9+ Hepatocytes during Liver Regeneration Stem Cell Reports, 2019;12(3):624-638. 2019 [PMID: 30773487] (IHC, Mouse) IHC Mouse
G Eelen, C Dubois, AR Cantelmo, J Goveia, U Brüning, M DeRan, G Jarugumill, J van Rijsse, G Saladino, F Comitani, A Zecchin, S Rocha, R Chen, H Huang, S Vandekeere, J Kalucka, C Lange, F Morales-Ro, B Cruys, L Treps, L Ramer, S Vinckier, K Brepoels, S Wyns, J Souffreau, L Schoonjans, WH Lamers, Y Wu, J Haustraete, J Hofkens, S Liekens, R Cubbon, B Ghesquière, M Dewerchin, FL Gervasio, X Li, JD van Buul, X Wu, P Carmeliet Role of glutamine synthetase in angiogenesis beyond glutamine synthesis Nature, 2018;561(7721):63-69. 2018 [PMID: 30158707] (IHC, Mouse) IHC Mouse
Show All 40 Publications.

Reviews for VE-Cadherin Antibody (AF1002) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using AF1002:
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Images Ratings Applications Species Date Details
 VE-Cadherin AF1002
reviewed by:
Bhavin Shah
Mouse 04/28/2020


Sample TestedEmbryonic brain


CommentsTissue sections were thawed and blocked in 10% NDS in PBST for 2 hours.
Primary &#40; 4degrees Overnight &#41; and secondary &#40;2 hours at RT&#41; were prepared in the same blocking buffer.
Immunohistochemistry-Frozen VE-Cadherin AF1002
reviewed by:
Hongyan Zhao
IHC-Fr Mouse 03/12/2015


Sample TestedMouse
Western Blot VE-Cadherin AF1002
reviewed by:
Hongyan Zhao
WB Mouse 02/25/2015


ApplicationWestern Blot
Sample TestedProtein


Blocking Details5% skim milk solution in TBS-T, Room temperature

Primary Anitbody

Dilution RatioAF1002, 1/1000 dilution, 4C,overnight, in 5% skim milk solution in TBS-T

Secondary Antibody

Secondary Descriptionsecondary anti-goat biotin conjugated antibody
Secondary Manufacturer Cat#Vector laboratories, BA-5000
Secondary Concentration1/2500 dilution


Detection Notesdetected using streptavidin conjugated IRDye 800 CW for 20 minutes at room temp,and scanned using LI-COR Odyssey imaging system

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Secondary Antibodies


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Biotin BAF1002

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Recent Reviews


Bhavin Shah
Species: Mouse

Hongyan Zhao
Application: IHC-Fr
Species: Mouse

Hongyan Zhao
Application: WB
Species: Mouse


Gene Symbol CDH5