VE-Cadherin Antibody [Unconjugated]

Western blot shows lysates of mouse lung tissue and bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse VE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1002) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for VE‑Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

VE‑Cadherin was detected in immersion fixed paraffin-embedded sections of mouse heart using Goat Anti-Mouse VE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1002) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western lane view shows lysates of mouse lung tissue, loaded at 0.2 mg/mL. Specific bands were detected for VE‑Cadherin at approximately 118, 146 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse VE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1002) . This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

• Reactivity: Mu
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

VE-Cadherin Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse VE-Cadherin
  Asp46-Gln592
  Accession # 2208309A
• Specificity: Detects mouse VE-Cadherin in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross‑reactivity with recombinant human VE-Cadherin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CDH5
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VE-Cadherin Antibody [Unconjugated]

• 7B4 antigen
• 7B4
• cadherin 5, type 2 (vascular endothelium)
• cadherin 5, type 2, VE-cadherin (vascular epithelium)
• Cadherin-5
• CD144 antigen
• CD144
• CDH5
• endothelial-specific cadherin
• FLJ17376
• Vascular endothelial cadherin
• VECadherin
• VE-Cadherin

Background

The cadherin (Ca⁺⁺-dependent adherence) superfamily is a large group of membrane-associated glycoproteins that engage in homotypic, calcium-dependent, cell-cell adhesion events. The superfamily can be divided into at least five major subfamilies based on molecule gene structure, and/or extracellular (EC) and intracellular domains (1-4). Subfamilies include classical/type I, atypical/type II, and desmosomal-related cadherins (1-3). VE-Cadherin (vascular endothelial cadherin; also cadherin-5 and CD144) is a 125 kDa atypical/type II subfamily cadherin. Its subfamily classification is based principally on its genomic structure, as its physical structure is notably divergent from other type II subfamily members (2, 3). Mouse VE-Cadherin is synthesized as a 784 amino acid (aa) type I transmembrane (TM) preproprotein that contains a 24 aa signal peptide, a 21 aa prosequence, a 554 aa extracellular region (ECR), a 21 aa TM segment, and a 164 aa cytoplasmic domain (5, 6). The ECR contains five Ca⁺⁺-binding cadherin domains that are approximately 105 aa in length. Cadherin domains are comprised of two beta ‑sheets that are oriented like bread in a sandwich. Although complex, the N-terminal cadherin domain mediates trans interactions, while the internal domains contribute to cis multimerizations (7). Mouse VE-Cadherin ECR is 92%, 77%, and 73% aa identical to rat, human and porcine VE-Cadherin ECR, respectively. VE-Cadherin is involved in the maintenance of endothelial permeability. In this regard, VE-Cadherin does not initiate new blood vessel formation; it maintains it once formed. Thus, when VE‑Cadherin is downregulated, cells part and permeability increases (8). Notably, VEGF is known to promote vascular leakage, and apparently does so by inducing a beta ‑arrestin-dependent endocytosis of VE-Cadherin (9). Part of this effect may be mediated by VE‑Cadherin itself which is reported to increase the membrane half-life of VEGF R2 (10). VE-Cadherin acts homotypically at sites of zonula adherens. On each expressing cell, it is proposed that VE-Cadherin first forms a trimer, which then dimerizes with a trimeric counterpart in-trans. Alternatively, two cis-dimers could act in-trans to generate homotypic binding (11). In addition to cell adhesion, VE‑Cadherin also is reported to mediate TGF-beta receptor assembly. When clustered, VE‑Cadherin enhances T beta RII/T beta RI assembly into an active receptor complex on endothelial cells (12). VE-Cadherin is expressed on endothelial cells, trophoblast cells, endothelial progenitor cells and embryonic hematopoietic cells (5, 8, 13, 14).

1. Patel, S.D. et al. (2007) Curr. Opin. Struct. Biol. 13:690.
2. Vestweber, D. (2008) Arterioscler. Thromb. Vasc. Biol. 28:223.
3. Vincent, P.A. et al. (2004) Am. J. Physiol. Cell. Physiol. 286:C987.
4. Cavallaro, U. et al. (2006) Exp. Cell Res. 312:659.
5. Breier, G. et al. (1996) Blood 87:630.
6. Huber, P. et al. (1996) Genomics 32:21.
7. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
8. Crosby, C.V. et al. (2005) Blood 105:2771.
9. Gavard, J. and J.S. Gutkind (2006) Nat. Cell Biol. 8:1223.
10. Calera, M.R. et al. (2004) Exp. Cell Res. 300:248.
11. Hewat, E.A. et al. (2007) J. Mol. Biol. 365:744.
12. Rudini, N. et al. (2008) EMBO J. 27:993.
13. Kogata, N. et al. (2006) Circ. Res. 98:897.
14. Ema, M. et al. (2006) Blood 108:4018.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VE-Cadherin Antibody (AF1002)(57)

Publications using AF1002

• W Zhang, Z Xu, X Hao, T He, J Li, Y Shen, K Liu, Y Gao, J Liu, D Edwards, AM Muscarella, L Wu, L Yu, L Xu, X Chen, YH Wu, IL Bado, Y Ding, S Aguirre, H Wang, Z Gugala, RL Satcher, ST Wong, XH Zhang Bone metastasis initiation is coupled with bone remodeling through osteogenic differentiation of NG2+ cells Cancer Discovery, 2022;0(0):. 2022 [PMID: 36287038] (IHC, Transgenic Mouse)
• R Hashimoto, J Takahashi, K Shirakura, R Funatsu, K Kosugi, S Deguchi, M Yamamoto, Y Tsunoda, M Morita, K Muraoka, M Tanaka, T Kanbara, S Tanaka, S Tamiya, N Tokunoh, A Kawai, M Ikawa, C Ono, K Tachibana, M Kondoh, M Obana, Y Matsuura, A Ohsumi, T Noda, T Yamamoto, Y Yoshioka, YS Torisawa, H Date, Y Fujio, M Nagao, K Takayama, Y Okada SARS-CoV-2 disrupts respiratory vascular barriers by suppressing Claudin-5 expression Science Advances, 2022;8(38):eabo6783. 2022 [PMID: 36129989] (Western Blot, Human)
• M Moulis, SVM Runser, L Glorieux, N Dauguet, C Vanderaa, L Gatto, D Tyteca, P Henriet, FM Spagnoli, D Iber, CE Pierreux Identification and implication of tissue-enriched ligands in epithelial-endothelial crosstalk during pancreas development Scientific Reports, 2022;12(1):12498. 2022 [PMID: 35864120] (IHC, Mouse)
• N Tisch, C Mogler, A Stojanovic, R Luck, EA Korhonen, A Ellerkmann, H Adler, M Singhal, G Schermann, L Erkert, JV Patankar, A Karakatsan, AL Scherr, Y Fuchs, A Cerwenka, S Wirtz, BC Köhler, HG Augustin, C Becker, T Schmidt, C Ruiz de Al Caspase-8 in endothelial cells maintains gut homeostasis and prevents small bowel inflammation in mice Embo Molecular Medicine, 2022;0(0):e14121. 2022 [PMID: 35491615] (IHC, Mouse)
• A Tarafdar, N Wolska, C Krisp, H Schlüter, G Pula The amyloid peptide beta disrupts intercellular junctions and increases endothelial permeability in a NADPH oxidase 1-dependent manner Redox Biology, 2022;52(0):102287. 2022 [PMID: 35358850] (IHC/IF, Mouse)
• CN Duong, R Brückner, M Schmitt, AF Nottebaum, L Braun, MM Zu Brickwe, U Ipe, H Vom Bruch, HR Schöler, G Trapani, B Trappmann, MP Ebrahimkut, S Huveneers, J Rooij, N Ishiyama, M Ikura, D Vestweber Force-induced changes of alpha-catenin conformation stabilize vascular junctions independent of vinculin Journal of Cell Science, 2021;0(0):. 2021 [PMID: 34851405] (ICC, Mouse)
• JM Arpino, H Yin, EK Prescott, SCR Staples, Z Nong, F Li, J Chevalier, B Balint, C O'Neil, R Mortuza, S Milkovich, JJ Lee, D Lorusso, M Sandig, DW Hamilton, DW Holdsworth, TL Poepping, CG Ellis, JG Pickering Low-flow intussusception and metastable VEGFR2 signaling launch angiogenesis in ischemic muscle Science Advances, 2021;7(48):eabg9509. 2021 [PMID: 34826235] (IHC, Mouse)
• S Heino, S Fang, M Lähde, J Högström, S Nassiri, A Campbell, D Flanagan, A Raven, M Hodder, N Nasreddin, HH Xue, M Delorenzi, S Leedham, TV Petrova, O Sansom, K Alitalo Lef1 restricts ectopic crypt formation and tumor cell growth in intestinal adenomas Science Advances, 2021;7(47):eabj0512. 2021 [PMID: 34788095] (IHC, Mouse)
• M Mesnieres, AM Böhm, N Peredo, D Trompet, R Valle-Tenn, M Bajaj, N Corthout, E Nefyodova, R Cardoen, P Baatsen, S Munck, A Nagy, JJ Haigh, S Khurana, CM Verfaillie, C Maes Fetal hematopoietic stem cell homing is controlled by VEGF regulating the integrity and oxidative status of the stromal-vascular bone marrow niches Cell Reports, 2021;36(8):109618. 2021 [PMID: 34433017] (IHC, Mouse)
• S Selbonne, C Madjene, B Salmon, Y Boulaftali, MC Bouton, V Arocas Protease nexin-1 deficiency increases mouse hindlimb neovascularisation following ischemia and accelerates femoral artery perfusion Scientific Reports, 2021;11(1):13412. 2021 [PMID: 34183729] (IHC, Mouse)
• M Richards, S Pal, E Sjöberg, P Martinsson, L Venkatrama, L Claesson-W Intra-vessel heterogeneity establishes enhanced sites of macromolecular leakage downstream of laminin &amp;alpha5 Cell Reports, 2021;35(12):109268. 2021 [PMID: 34161758] (IHC, Mouse)
• E Russo, P Runge, NH Jahromi, H Naboth, A Landtwing, R Montecchi, N Leicht, MC Hunter, Y Takai, C Halin CD112 Regulates Angiogenesis and T Cell Entry into the Spleen Cells, 2021;10(1):. 2021 [PMID: 33467729] (ICC, Mouse)
• SJ Jeong, MJ Cho, NY Ko, S Kim, IH Jung, JK Min, SH Lee, JG Park, GT Oh Deficiency of peroxiredoxin 2 exacerbates angiotensin II-induced abdominal aortic aneurysm Exp. Mol. Med., 2020;0(0):. 2020 [PMID: 32929220] (Western Blot, Mouse)
• A Tabibian, S Ghaffari, DA Vargas, H Van Ooster, EAV Jones Simulating flow induced migration in vascular remodelling PLoS Comput. Biol., 2020;16(8):e1007874. 2020 [PMID: 32822340] (IHC, Mouse)
• A Tabibian, S Ghaffari, DA Vargas, H Van Ooster, EAV Jones Simulating flow induced migration in vascular remodelling PLoS Comput. Biol., 2020;16(8):e1007874. 2020 [PMID: 32822340] (IHC, Mouse)
• SP Hong, MJ Yang, H Cho, I Park, H Bae, K Choe, SH Suh, RH Adams, K Alitalo, D Lim, GY Koh Distinct fibroblast subsets regulate lacteal integrity through YAP/TAZ-induced VEGF-C in intestinal villi Nat Commun, 2020;11(1):4102. 2020 [PMID: 32796823] (IHC, Mouse)
• SP Hong, MJ Yang, H Cho, I Park, H Bae, K Choe, SH Suh, RH Adams, K Alitalo, D Lim, GY Koh Distinct fibroblast subsets regulate lacteal integrity through YAP/TAZ-induced VEGF-C in intestinal villi Nat Commun, 2020;11(1):4102. 2020 [PMID: 32796823] (IHC, Mouse)
• B Lee, H Shin, JE Oh, J Park, M Park, SC Yang, JH Jun, SH Hong, H Song, HJ Lim An autophagic deficit in the uterine vessel microenvironment provokes hyperpermeability through deregulated VEGFA, NOS1, and CTNNB1 Autophagy, 2020;0(0):1-18. 2020 [PMID: 32579471] (Western Blot, Mouse)
• JR Krambs, G Abou Ezzi, JC Yao, DC Link Canonical signaling by TGF family members in mesenchymal stromal cells is dispensable for hematopoietic niche maintenance under basal and stress conditions PLoS ONE, 2020;15(5):e0233751. 2020 [PMID: 32470079] (IHC, Mouse)
• Z Wang, F Wang, J Zhong, T Zhu, Y Zheng, T Zhao, Q Xie, F Ma, R Li, Q Tang, F Xu, X Tian, J Zhu Using apelin-based synthetic Notch receptors to detect angiogenesis and treat solid tumors Nat Commun, 2020;11(1):2163. 2020 [PMID: 32358530] (ICC, Mouse)
• KL Betterman, DL Sutton, GA Secker, J Kazenwadel, A Oszmiana, L Lim, N Miura, L Sorokin, BM Hogan, ML Kahn, H McNeill, NL Harvey Atypical cadherin Fat4 orchestrates lymphatic endothelial cell polarity in response to flow J. Clin. Invest., 2020;0(0):. 2020 [PMID: 32182215] (IHC, Mouse)
• TK Darling, PN Mimche, C Bray, B Umaru, LM Brady, C Stone, CE Eboumbou M, TE Lane, LS Ayong, TJ Lamb EphA2 contributes to disruption of the blood-brain barrier in cerebral malaria PLoS Pathog., 2020;16(1):e1008261. 2020 [PMID: 31999807] (IHC, Mouse)
• JH Wu, YN Li, AQ Chen, CD Hong, CL Zhang, HL Wang, YF Zhou, PC Li, Y Wang, L Mao, YP Xia, QW He, HJ Jin, ZY Yue, B Hu Inhibition of Sema4D/PlexinB1 signaling alleviates vascular dysfunction in diabetic retinopathy EMBO Mol Med, 2020;12(2):e10154. 2020 [PMID: 31943789] (Western Blot, Mouse)
• G Chiaverina, L di Blasio, V Monica, M Accardo, M Palmiero, B Peracino, M Vara-Messl, A Puliafito, L Primo Dynamic Interplay between Pericytes and Endothelial Cells during Sprouting Angiogenesis Cells, 2019;8(9):. 2019 [PMID: 31546913] (IHC, Mouse)
• J Zhang, T Supakornde, JR Krambs, M Rao, G Abou-Ezzi, RY Ye, S Li, K Trinkaus, DC Link Bone marrow dendritic cells regulate hematopoietic stem/progenitor cell trafficking J. Clin. Invest., 2019;129(7):2920-2931. 2019 [PMID: 31039135] (IHC, Mouse)
• X Han, Y Wang, W Pu, X Huang, L Qiu, Y Li, W Yu, H Zhao, X Liu, L He, L Zhang, Y Ji, J Lu, KO Lui, B Zhou Lineage Tracing Reveals the Bipotency of SOX9+ Hepatocytes during Liver Regeneration Stem Cell Reports, 2019;12(3):624-638. 2019 [PMID: 30773487] (IHC, Mouse)
• G Eelen, C Dubois, AR Cantelmo, J Goveia, U Brüning, M DeRan, G Jarugumill, J van Rijsse, G Saladino, F Comitani, A Zecchin, S Rocha, R Chen, H Huang, S Vandekeere, J Kalucka, C Lange, F Morales-Ro, B Cruys, L Treps, L Ramer, S Vinckier, K Brepoels, S Wyns, J Souffreau, L Schoonjans, WH Lamers, Y Wu, J Haustraete, J Hofkens, S Liekens, R Cubbon, B Ghesquière, M Dewerchin, FL Gervasio, X Li, JD van Buul, X Wu, P Carmeliet Role of glutamine synthetase in angiogenesis beyond glutamine synthesis Nature, 2018;561(7721):63-69. 2018 [PMID: 30158707] (IHC, Mouse)
• Stephan Niland, Dorde Komljenov, Jadranka Macas, Thilo Bracht, Tobias Bäuerle, Stefan Liebner, Johannes A Eble Rhodocetin-?? selectively breaks the endothelial barrier of the tumor vasculature in HT1080 fibrosarcoma and A431 epidermoid carcinoma tumor models. Oncotarget, 2018;0(0):1949-2553. 2018 [PMID: 29854288] (IHC, Mouse)
• KG Blecharz-L, V Prinz, M Burek, D Frey, T Schenkel, SM Krug, M Fromm, P Vajkoczy Gelatinolytic activity of autocrine matrix metalloproteinase-9 leads to endothelial de-arrangement in Moyamoya disease J. Cereb. Blood Flow Metab., 2018;0(0):271678X187684. 2018 [PMID: 29633884] (ICC, Mouse)
• I Milo, R Blecher-Go, Z Barnett-It, R Bar-Ziv, O Tal, I Gurevich, T Feferman, I Drexler, I Amit, P Bousso, G Shakhar The bone marrow is patrolled by NK cells that are primed and expand in response to systemic viral activation Eur. J. Immunol., 2018;0(0):. 2018 [PMID: 29624673] (IHC-Fr, Mouse)
• L Guo, H Akahori, E Harari, SL Smith, R Polavarapu, V Karmali, F Otsuka, RL Gannon, RE Braumann, MH Dickinson, A Gupta, AL Jenkins, MJ Lipinski, J Kim, P Chhour, PS de Vries, H Jinnouchi, R Kutys, H Mori, MD Kutyna, S Torii, A Sakamoto, CU Choi, Q Cheng, ML Grove, MA Sawan, Y Zhang, Y Cao, FD Kolodgie, DP Cormode, DE Arking, E Boerwinkle, AC Morrison, J Erdmann, N Sotoodehni, R Virmani, AV Finn CD163+ macrophages promote angiogenesis and vascular permeability accompanied by inflammation in atherosclerosis J. Clin. Invest., 2018;0(0):. 2018 [PMID: 29457790] (IHC, Human)
• J Cao, M Ehling, S März, J Seebach, K Tarbashevi, T Sixta, ME Pitulescu, AC Werner, B Flach, E Montanez, E Raz, RH Adams, H Schnittler Polarized actin and VE-cadherin dynamics regulate junctional remodelling and cell migration during sprouting angiogenesis Nat Commun, 2017;8(1):2210. 2017 [PMID: 29263363] (IHC, Mouse)
• KM Tsang, JS Hyun, KT Cheng, M Vargas, D Mehta, M Ushio-Fuka, L Zou, KV Pajcini, J Rehman, AB Malik Embryonic Stem Cell Differentiation to Functional Arterial Endothelial Cells through Sequential Activation of ETV2 and NOTCH1 Signaling by HIF1? Stem Cell Reports, 2017;0(0):. 2017 [PMID: 28781077] (Flow Cytometry, Mouse)
• AJ Mead, WH Neo, N Barkas, S Matsuoka, A Giustacchi, R Facchini, S Thongjuea, L Jamieson, CAG Booth, N Fordham, C Di Genua, D Atkinson, O Chowdhury, E Repapi, N Gray, S Kharazi, SA Clark, T Bouriez, P Woll, T Suda, C Nerlov, SEW Jacobsen Niche-mediated depletion of the normal hematopoietic stem cell reservoir by Flt3-ITD-induced myeloproliferation J. Exp. Med., 2017;0(0):. 2017 [PMID: 28637883] (IHC, Mouse)
• L Tian, A Goldstein, H Wang, H Ching Lo, I Sun Kim, T Welte, K Sheng, LE Dobrolecki, X Zhang, N Putluri, TL Phung, SA Mani, F Stossi, A Sreekumar, MA Mancini, WK Decker, C Zong, MT Lewis, XH Zhang Mutual regulation of tumour vessel normalization and immunostimulatory reprogramming Nature, 2017;0(0):. 2017 [PMID: 28371798] (IHC, Mouse)
• HDL activation of endothelial sphingosine-1-phosphate receptor-1 (S1P1) promotes regeneration and suppresses fibrosis in the liver JCI Insight, 2016;1(21):e87058. 2016 [PMID: 28018969] (IHC-Fr, Mouse)
• Endothelial cells are progenitors of cardiac pericytes and vascular smooth muscle cells Nat Commun, 2016;7(0):12422. 2016 [PMID: 27516371] (IHC-Fr, Mouse)
• Jingzhu Zhang Targeting of Mesenchymal Stromal Cells by Cre-Recombinase Transgenes Commonly Used to Target Osteoblast Lineage Cells J Bone Miner Res, 2016;0(0):. 2016 [PMID: 27237054] (IHC, Mouse)
• Liu Q, Yang R, Huang X, Zhang H, He L, Zhang L, Tian X, Nie Y, Hu S, Yan Y, Zhang L, Qiao Z, Wang Q, Lui K, Zhou B Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes. Cell Res, 0;26(1):119-30. 0 [PMID: 26634606] (IHC-Fr, Mouse)
• Shen Y, Cheng F, Sharma M, Merkulova Y, Raithatha S, Parkinson L, Zhao H, Westendorf K, Bohunek L, Bozin T, Hsu I, Ang L, Williams S, Bleackley R, Eriksson J, Seidman M, McManus B, Granville D Granzyme B Deficiency Protects against Angiotensin II-Induced Cardiac Fibrosis. Am J Pathol, 2016;186(1):87-100. 2016 [PMID: 26610869] (Western Blot, Mouse)
• Yin H, Frontini M, Arpino J, Nong Z, O'Neil C, Xu Y, Balint B, Ward A, Chakrabarti S, Ellis C, Gros R, Pickering J Fibroblast Growth Factor 9 Imparts Hierarchy and Vasoreactivity to the Microcirculation of Renal Tumors and Suppresses Metastases. J Biol Chem, 2015;290(36):22127-42. 2015 [PMID: 26183774] (IHC-P, Mouse)
• Zang G, Gustafsson K, Jamalpour M, Hong J, Genove G, Welsh M Vascular dysfunction and increased metastasis of B16F10 melanomas in Shb deficient mice as compared with their wild type counterparts. BMC Cancer, 2015;15(0):234. 2015 [PMID: 25885274] (IHC, Mouse)
• Samarelli A, Riccitelli E, Bizzozero L, Silveira T, Seano G, Pergolizzi M, Vitagliano G, Cascone I, Carpentier G, Bottos A, Primo L, Bussolino F, Arese M Neuroligin 1 induces blood vessel maturation by cooperating with the alpha6 integrin. J Biol Chem, 2014;289(28):19466-76. 2014 [PMID: 24860089] (IHC, Mouse)
• Stoler-Barak , Liat, Moussion , Christin, Shezen , Elias, Hatzav , Miki, Sixt , Michael, Alon , Ronen Blood vessels pattern heparan sulfate gradients between their apical and basolateral aspects. PLoS ONE, 2014;9(1):e85699. 2014 [PMID: 24465652] (IHC, Mouse)
• Kohler , Erin E, Wary , Kishore, Li , Fei, Chatterjee , Ishita, Urao , Norifumi, Toth , Peter T, Ushio-Fukai , Masuko, Rehman , Jalees, Park , Changwon, Malik , Asrar B Flk1+ and VE-cadherin+ endothelial cells derived from iPSCs recapitulates vascular development during differentiation and display similar angiogenic potential as ESC-derived cells. PLoS ONE, 2013;8(12):e85549. 2013 [PMID: 24386480] (Flow Cytometry, Mouse)
• Lu , Zhi Hong, Kaliberov , Sergey, Sohn , Rebecca, Kaliberova , Lyudmila, Curiel , David T, Arbeit , Jeffrey Transcriptional targeting of primary and metastatic tumor neovasculature by an adenoviral type 5 roundabout4 vector in mice. PLoS ONE, 2013;8(12):e83933. 2013 [PMID: 24376772] (Western Blot, Mouse)
• Bowles J, Secker G, Nguyen C, Kazenwadel J, Truong V, Frampton E, Curtis C, Skoczylas R, Davidson T, Miura N, Hong Y, Koopman P, Harvey N, Francois M Control of retinoid levels by CYP26B1 is important for lymphatic vascular development in the mouse embryo. Dev Biol, 2014;386(1):25-33. 2014 [PMID: 24361262] (IHC-Fr, Mouse)
• Ding B, Cao Z, Lis R, Nolan D, Guo P, Simons M, Penfold M, Shido K, Rabbany S, Rafii S Divergent angiocrine signals from vascular niche balance liver regeneration and fibrosis. Nature, 2014;505(7481):97-102. 2014 [PMID: 24256728] (IHC, Mouse)
• Sun Z, Li X, Massena S, Kutschera S, Padhan N, Gualandi L, Sundvold-Gjerstad V, Gustafsson K, Choy WW, Zang G, Quach M, Jansson L, Phillipson M, Abid MR, Spurkland A, Claesson-Welsh L VEGFR2 induces c-Src signaling and vascular permeability in vivo via the adaptor protein TSAd. J. Exp. Med., 2012;209(7):1363-77. 2012 [PMID: 22689825] (Western Blot, Mouse)
• Marrotte EJ, Chen DD, Hakim JS Manganese superoxide dismutase expression in endothelial progenitor cells accelerates wound healing in diabetic mice. J. Clin. Invest., 2010;120(12):4207-19. 2010 [PMID: 21060152] (Flow Cytometry, Mouse)
• Butler JM, Nolan DJ, Vertes EL, Varnum-Finney B, Kobayashi H, Hooper AT, Seandel M, Shido K, White IA, Kobayashi M, Witte L, May C, Shawber C, Kimura Y, Kitajewski J, Rosenwaks Z, Bernstein ID, Rafii S Endothelial cells are essential for the self-renewal and repopulation of Notch-dependent hematopoietic stem cells. Cell Stem Cell, 2010;6(3):251-64. 2010 [PMID: 20207228] (ICC, Mouse)
• Funa NS, Kriz V, Zang G, Calounova G, Akerblom B, Mares J, Larsson E, Sun Y, Betsholtz C, Welsh M Dysfunctional microvasculature as a consequence of shb gene inactivation causes impaired tumor growth. Cancer Res., 2009;69(5):2141-8. 2009 [PMID: 19223532] (IHC, Mouse)
• Mazzone M, Dettori D, Leite de Oliveira R, Loges S, Schmidt T, Jonckx B, Tian YM, Lanahan AA, Pollard P, Ruiz de Almodovar C, De Smet F, Vinckier S, Aragones J, Debackere K, Luttun A, Wyns S, Jordan B, Pisacane A, Gallez B, Lampugnani MG, Dejana E, Simons M, Ratcliffe P, Maxwell P, Carmeliet P Heterozygous deficiency of PHD2 restores tumor oxygenation and inhibits metastasis via endothelial normalization. Cell, 2009;136(5):839-51. 2009 [PMID: 19217150] (ICC, Mouse)
• Cordey M, Limacher M, Kobel S, Taylor V, Lutolf MP Enhancing the reliability and throughput of neurosphere culture on hydrogel microwell arrays. Stem Cells, 2008;26(10):2586-94. 2008 [PMID: 18669905] (ICC, Mouse)
• Watanabe M, Boyer JL, Hackett NR, Qiu J, Crystal RG Genetic delivery of the murine equivalent of bevacizumab (avastin), an anti-vascular endothelial growth factor monoclonal antibody, to suppress growth of human tumors in immunodeficient mice. Hum. Gene Ther., 2008;19(3):300-10. 2008 [PMID: 18324912] (IHC-P, Mouse)
• Li X, Edholm D, Lanner F, Breier G, Farnebo F, Dimberg A, Claesson-Welsh L Lentiviral rescue of vascular endothelial growth factor receptor-2 expression in flk1-/- embryonic stem cells shows early priming of endothelial precursors. Stem Cells, 2007;25(12):2987-95. 2007 [PMID: 17702981] (ICC, Mouse)
• Rutland CS, Latunde-Dada AO, Thorpe A, Plant R, Langley-Evans S, Leach L Effect of gestational nutrition on vascular integrity in the murine placenta. Placenta, 2006;28(7):734-42. 2006 [PMID: 16930688] (IHC, Mouse)

Reviews for VE-Cadherin Antibody (AF1002) (3)

Average Rating: 4.3

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer Immunofluorescence-frozen sections Mouse 04/28/2020

Summary

• Application: Other
• Sample Tested: Embryonic brain
• Species: Mouse
• Lot: FQI0117051

Comments

• Comments: Tissue sections were thawed and blocked in 10% NDS in PBST for 2 hours.
  Primary ( 4degrees Overnight ) and secondary (2 hours at RT) were prepared in the same blocking buffer.

reviewed by: Verified Customer IHC-Fr Mouse 03/12/2015

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Mouse
• Species: Mouse
• Lot: FQ10113071

reviewed by: Verified Customer WB Mouse 02/25/2015

Summary

• Application: Western Blot
• Sample Tested: Protein
• Species: Mouse
• Lot: FQ10113071

Bioinformatics

• Gene Symbol: CDH5
• Uniprot:
  • 2208309A()