VE-Cadherin Antibody [Unconjugated]

Images

 
VE‑Cadherin was detected in immersion fixed paraffin-embedded sections of mouse heart using Goat Anti-Mouse VE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1002) at 3 µg/mL for 1 hour ...read more
Western blot shows lysates of mouse lung tissue and bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse VE‑Cadherin Antigen Affinity-purified Polyclonal Antibody ...read more
Simple Western lane view shows lysates of mouse lung tissue, loaded at 0.2 mg/mL. Specific bands were detected for VE‑Cadherin at approximately 118, 146 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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VE-Cadherin Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse VE-Cadherin
Asp46-Gln592
Accession # 2208309A
Specificity
Detects mouse VE-Cadherin in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross‑reactivity with recombinant human VE-Cadherin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CDH5
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 3-25 ug/mL
Reviewed Applications
Read 2 Reviews rated 5
using
AF1002 in the following applications:

Publications
Read Publications using
AF1002 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VE-Cadherin Antibody [Unconjugated]

  • 7B4 antigen
  • 7B4
  • cadherin 5, type 2 (vascular endothelium)
  • cadherin 5, type 2, VE-cadherin (vascular epithelium)
  • Cadherin-5
  • CD144 antigen
  • CD144
  • CDH5
  • endothelial-specific cadherin
  • FLJ17376
  • Vascular endothelial cadherin
  • VECadherin
  • VE-Cadherin

Background

The cadherin (Ca++-dependent adherence) superfamily is a large group of membrane-associated glycoproteins that engage in homotypic, calcium-dependent, cell-cell adhesion events. The superfamily can be divided into at least five major subfamilies based on molecule gene structure, and/or extracellular (EC) and intracellular domains (1-4). Subfamilies include classical/type I, atypical/type II, and desmosomal-related cadherins (1-3). VE-Cadherin (vascular endothelial cadherin; also cadherin-5 and CD144) is a 125 kDa atypical/type II subfamily cadherin. Its subfamily classification is based principally on its genomic structure, as its physical structure is notably divergent from other type II subfamily members (2, 3). Mouse VE-Cadherin is synthesized as a 784 amino acid (aa) type I transmembrane (TM) preproprotein that contains a 24 aa signal peptide, a 21 aa prosequence, a 554 aa extracellular region (ECR), a 21 aa TM segment, and a 164 aa cytoplasmic domain (5, 6). The ECR contains five Ca++-binding cadherin domains that are approximately 105 aa in length. Cadherin domains are comprised of two beta ‑sheets that are oriented like bread in a sandwich. Although complex, the N-terminal cadherin domain mediates trans interactions, while the internal domains contribute to cis multimerizations (7). Mouse VE-Cadherin ECR is 92%, 77%, and 73% aa identical to rat, human and porcine VE-Cadherin ECR, respectively. VE-Cadherin is involved in the maintenance of endothelial permeability. In this regard, VE-Cadherin does not initiate new blood vessel formation; it maintains it once formed. Thus, when VE‑Cadherin is downregulated, cells part and permeability increases (8). Notably, VEGF is known to promote vascular leakage, and apparently does so by inducing a beta ‑arrestin-dependent endocytosis of VE-Cadherin (9). Part of this effect may be mediated by VE‑Cadherin itself which is reported to increase the membrane half-life of VEGF R2 (10). VE-Cadherin acts homotypically at sites of zonula adherens. On each expressing cell, it is proposed that VE-Cadherin first forms a trimer, which then dimerizes with a trimeric counterpart in-trans. Alternatively, two cis-dimers could act in-trans to generate homotypic binding (11). In addition to cell adhesion, VE‑Cadherin also is reported to mediate TGF-beta receptor assembly. When clustered, VE‑Cadherin enhances T beta RII/T beta RI assembly into an active receptor complex on endothelial cells (12). VE-Cadherin is expressed on endothelial cells, trophoblast cells, endothelial progenitor cells and embryonic hematopoietic cells (5, 8, 13, 14).

  1. Patel, S.D. et al. (2007) Curr. Opin. Struct. Biol. 13:690.
  2. Vestweber, D. (2008) Arterioscler. Thromb. Vasc. Biol. 28:223.
  3. Vincent, P.A. et al. (2004) Am. J. Physiol. Cell. Physiol. 286:C987.
  4. Cavallaro, U. et al. (2006) Exp. Cell Res. 312:659.
  5. Breier, G. et al. (1996) Blood 87:630.
  6. Huber, P. et al. (1996) Genomics 32:21.
  7. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
  8. Crosby, C.V. et al. (2005) Blood 105:2771.
  9. Gavard, J. and J.S. Gutkind (2006) Nat. Cell Biol. 8:1223.
  10. Calera, M.R. et al. (2004) Exp. Cell Res. 300:248.
  11. Hewat, E.A. et al. (2007) J. Mol. Biol. 365:744.
  12. Rudini, N. et al. (2008) EMBO J. 27:993.
  13. Kogata, N. et al. (2006) Circ. Res. 98:897.
  14. Ema, M. et al. (2006) Blood 108:4018.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VE-Cadherin Antibody (AF1002)(31)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Western Blot.


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Flow Cytometry
(3)
ICC
(4)
IHC
(14)
IHC-Fr
(5)
IHC-P
(2)
Western Blot
(3)
All Applications
Filter By Species
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(1)
Mouse
(30)
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Showing Publications 1 - 10 of 31. Show All 31 Publications.
Publications using AF1002 Applications Species
X Han, Y Wang, W Pu, X Huang, L Qiu, Y Li, W Yu, H Zhao, X Liu, L He, L Zhang, Y Ji, J Lu, KO Lui, B Zhou Lineage Tracing Reveals the Bipotency of SOX9+ Hepatocytes during Liver Regeneration Stem Cell Reports, 2019;12(3):624-638. 2019 [PMID: 30773487] (IHC, Mouse) IHC Mouse
G Eelen, C Dubois, AR Cantelmo, J Goveia, U Brüning, M DeRan, G Jarugumill, J van Rijsse, G Saladino, F Comitani, A Zecchin, S Rocha, R Chen, H Huang, S Vandekeere, J Kalucka, C Lange, F Morales-Ro, B Cruys, L Treps, L Ramer, S Vinckier, K Brepoels, S Wyns, J Souffreau, L Schoonjans, WH Lamers, Y Wu, J Haustraete, J Hofkens, S Liekens, R Cubbon, B Ghesquière, M Dewerchin, FL Gervasio, X Li, JD van Buul, X Wu, P Carmeliet Role of glutamine synthetase in angiogenesis beyond glutamine synthesis Nature, 2018;561(7721):63-69. 2018 [PMID: 30158707] (IHC, Mouse) IHC Mouse
Stephan Niland, Dorde Komljenov, Jadranka Macas, Thilo Bracht, Tobias Bäuerle, Stefan Liebner, Johannes A Eble Rhodocetin-?? selectively breaks the endothelial barrier of the tumor vasculature in HT1080 fibrosarcoma and A431 epidermoid carcinoma tumor models. Oncotarget, 2018;0(0):1949-2553. 2018 [PMID: 29854288] (IHC, Mouse) IHC Mouse
I Milo, R Blecher-Go, Z Barnett-It, R Bar-Ziv, O Tal, I Gurevich, T Feferman, I Drexler, I Amit, P Bousso, G Shakhar The bone marrow is patrolled by NK cells that are primed and expand in response to systemic viral activation Eur. J. Immunol., 2018;0(0):. 2018 [PMID: 29624673] (IHC-Fr, Mouse) IHC-Fr Mouse
L Guo, H Akahori, E Harari, SL Smith, R Polavarapu, V Karmali, F Otsuka, RL Gannon, RE Braumann, MH Dickinson, A Gupta, AL Jenkins, MJ Lipinski, J Kim, P Chhour, PS de Vries, H Jinnouchi, R Kutys, H Mori, MD Kutyna, S Torii, A Sakamoto, CU Choi, Q Cheng, ML Grove, MA Sawan, Y Zhang, Y Cao, FD Kolodgie, DP Cormode, DE Arking, E Boerwinkle, AC Morrison, J Erdmann, N Sotoodehni, R Virmani, AV Finn CD163+ macrophages promote angiogenesis and vascular permeability accompanied by inflammation in atherosclerosis J. Clin. Invest., 2018;0(0):. 2018 [PMID: 29457790] (IHC, Human) IHC Human
J Cao, M Ehling, S März, J Seebach, K Tarbashevi, T Sixta, ME Pitulescu, AC Werner, B Flach, E Montanez, E Raz, RH Adams, H Schnittler Polarized actin and VE-cadherin dynamics regulate junctional remodelling and cell migration during sprouting angiogenesis Nat Commun, 2017;8(1):2210. 2017 [PMID: 29263363] (IHC, Mouse) IHC Mouse
KM Tsang, JS Hyun, KT Cheng, M Vargas, D Mehta, M Ushio-Fuka, L Zou, KV Pajcini, J Rehman, AB Malik Embryonic Stem Cell Differentiation to Functional Arterial Endothelial Cells through Sequential Activation of ETV2 and NOTCH1 Signaling by HIF1? Stem Cell Reports, 2017;0(0):. 2017 [PMID: 28781077] (Flow Cytometry, Mouse) Flow Cytometry Mouse
AJ Mead, WH Neo, N Barkas, S Matsuoka, A Giustacchi, R Facchini, S Thongjuea, L Jamieson, CAG Booth, N Fordham, C Di Genua, D Atkinson, O Chowdhury, E Repapi, N Gray, S Kharazi, SA Clark, T Bouriez, P Woll, T Suda, C Nerlov, SEW Jacobsen Niche-mediated depletion of the normal hematopoietic stem cell reservoir by Flt3-ITD-induced myeloproliferation J. Exp. Med., 2017;0(0):. 2017 [PMID: 28637883] (IHC, Mouse) IHC Mouse
L Tian, A Goldstein, H Wang, H Ching Lo, I Sun Kim, T Welte, K Sheng, LE Dobrolecki, X Zhang, N Putluri, TL Phung, SA Mani, F Stossi, A Sreekumar, MA Mancini, WK Decker, C Zong, MT Lewis, XH Zhang Mutual regulation of tumour vessel normalization and immunostimulatory reprogramming Nature, 2017;0(0):. 2017 [PMID: 28371798] (IHC, Mouse) IHC Mouse
HDL activation of endothelial sphingosine-1-phosphate receptor-1 (S1P1) promotes regeneration and suppresses fibrosis in the liver JCI Insight, 2016;1(21):e87058. 2016 [PMID: 28018969] (IHC-Fr, Mouse) IHC-Fr Mouse
Show All 31 Publications.

Reviews for VE-Cadherin Antibody (AF1002) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF1002:
Filter by Applications
IHC-Fr
(1)
WB
(1)
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Mouse
(2)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen VE-Cadherin AF1002
Enlarge
reviewed by:
Hongyan Zhao
IHC-Fr Mouse 03/12/2015
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse
SpeciesMouse
LotFQ10113071
Western Blot VE-Cadherin AF1002
Enlarge
reviewed by:
Hongyan Zhao
WB Mouse 02/25/2015
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Summary

ApplicationWestern Blot
Sample TestedProtein
SpeciesMouse
LotFQ10113071

Blocking

Blocking Details5% skim milk solution in TBS-T, Room temperature

Primary Anitbody

Dilution RatioAF1002, 1/1000 dilution, 4C,overnight, in 5% skim milk solution in TBS-T

Secondary Antibody

Secondary Descriptionsecondary anti-goat biotin conjugated antibody
Secondary Manufacturer Cat#Vector laboratories, BA-5000
Secondary Concentration1/2500 dilution

Details

Detection Notesdetected using streptavidin conjugated IRDye 800 CW for 20 minutes at room temp,and scanned using LI-COR Odyssey imaging system

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Video Protocols

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FAQs for VE-Cadherin Antibody (AF1002) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF1002

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
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Hongyan Zhao
03/12/2015
Application: IHC-Fr
Species: Mouse

Hongyan Zhao
02/25/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol CDH5
Uniprot