VE-Cadherin Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse lung tissue and bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse VE‑Cadherin Antigen Affinity-purified Polyclonal Antibody ...read more
VE‑Cadherin was detected in immersion fixed paraffin-embedded sections of mouse heart using Goat Anti-Mouse VE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1002) at 3 µg/mL for 1 hour ...read more
Simple Western lane view shows lysates of mouse lung tissue, loaded at 0.2 mg/mL. Specific bands were detected for VE‑Cadherin at approximately 118, 146 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

VE-Cadherin Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse VE-Cadherin
Asp46-Gln592
Accession # 2208309A
Specificity
Detects mouse VE-Cadherin in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross‑reactivity with recombinant human VE-Cadherin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CDH5
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 3-25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.2 ug/mL
Reviewed Applications
Read 3 Reviews rated 4.3
using
AF1002 in the following applications:

Publications
Read Publications using
AF1002 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VE-Cadherin Antibody [Unconjugated]

  • 7B4 antigen
  • 7B4
  • cadherin 5, type 2 (vascular endothelium)
  • cadherin 5, type 2, VE-cadherin (vascular epithelium)
  • Cadherin-5
  • CD144 antigen
  • CD144
  • CDH5
  • endothelial-specific cadherin
  • FLJ17376
  • Vascular endothelial cadherin
  • VECadherin
  • VE-Cadherin

Background

The cadherin (Ca++-dependent adherence) superfamily is a large group of membrane-associated glycoproteins that engage in homotypic, calcium-dependent, cell-cell adhesion events. The superfamily can be divided into at least five major subfamilies based on molecule gene structure, and/or extracellular (EC) and intracellular domains (1-4). Subfamilies include classical/type I, atypical/type II, and desmosomal-related cadherins (1-3). VE-Cadherin (vascular endothelial cadherin; also cadherin-5 and CD144) is a 125 kDa atypical/type II subfamily cadherin. Its subfamily classification is based principally on its genomic structure, as its physical structure is notably divergent from other type II subfamily members (2, 3). Mouse VE-Cadherin is synthesized as a 784 amino acid (aa) type I transmembrane (TM) preproprotein that contains a 24 aa signal peptide, a 21 aa prosequence, a 554 aa extracellular region (ECR), a 21 aa TM segment, and a 164 aa cytoplasmic domain (5, 6). The ECR contains five Ca++-binding cadherin domains that are approximately 105 aa in length. Cadherin domains are comprised of two beta ‑sheets that are oriented like bread in a sandwich. Although complex, the N-terminal cadherin domain mediates trans interactions, while the internal domains contribute to cis multimerizations (7). Mouse VE-Cadherin ECR is 92%, 77%, and 73% aa identical to rat, human and porcine VE-Cadherin ECR, respectively. VE-Cadherin is involved in the maintenance of endothelial permeability. In this regard, VE-Cadherin does not initiate new blood vessel formation; it maintains it once formed. Thus, when VE‑Cadherin is downregulated, cells part and permeability increases (8). Notably, VEGF is known to promote vascular leakage, and apparently does so by inducing a beta ‑arrestin-dependent endocytosis of VE-Cadherin (9). Part of this effect may be mediated by VE‑Cadherin itself which is reported to increase the membrane half-life of VEGF R2 (10). VE-Cadherin acts homotypically at sites of zonula adherens. On each expressing cell, it is proposed that VE-Cadherin first forms a trimer, which then dimerizes with a trimeric counterpart in-trans. Alternatively, two cis-dimers could act in-trans to generate homotypic binding (11). In addition to cell adhesion, VE‑Cadherin also is reported to mediate TGF-beta receptor assembly. When clustered, VE‑Cadherin enhances T beta RII/T beta RI assembly into an active receptor complex on endothelial cells (12). VE-Cadherin is expressed on endothelial cells, trophoblast cells, endothelial progenitor cells and embryonic hematopoietic cells (5, 8, 13, 14).

  1. Patel, S.D. et al. (2007) Curr. Opin. Struct. Biol. 13:690.
  2. Vestweber, D. (2008) Arterioscler. Thromb. Vasc. Biol. 28:223.
  3. Vincent, P.A. et al. (2004) Am. J. Physiol. Cell. Physiol. 286:C987.
  4. Cavallaro, U. et al. (2006) Exp. Cell Res. 312:659.
  5. Breier, G. et al. (1996) Blood 87:630.
  6. Huber, P. et al. (1996) Genomics 32:21.
  7. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
  8. Crosby, C.V. et al. (2005) Blood 105:2771.
  9. Gavard, J. and J.S. Gutkind (2006) Nat. Cell Biol. 8:1223.
  10. Calera, M.R. et al. (2004) Exp. Cell Res. 300:248.
  11. Hewat, E.A. et al. (2007) J. Mol. Biol. 365:744.
  12. Rudini, N. et al. (2008) EMBO J. 27:993.
  13. Kogata, N. et al. (2006) Circ. Res. 98:897.
  14. Ema, M. et al. (2006) Blood 108:4018.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VE-Cadherin Antibody (AF1002)(57)

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 7 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, IHC/IF, Western Blot.


Filter By Application
Flow Cytometry
(3)
ICC
(8)
IHC
(31)
IHC-Fr
(5)
IHC-P
(2)
IHC/IF
(1)
Western Blot
(7)
All Applications
Filter By Species
Human
(2)
Mouse
(54)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 57. Show All 57 Publications.
Publications using AF1002 Applications Species
W Zhang, Z Xu, X Hao, T He, J Li, Y Shen, K Liu, Y Gao, J Liu, D Edwards, AM Muscarella, L Wu, L Yu, L Xu, X Chen, YH Wu, IL Bado, Y Ding, S Aguirre, H Wang, Z Gugala, RL Satcher, ST Wong, XH Zhang Bone metastasis initiation is coupled with bone remodeling through osteogenic differentiation of NG2+ cells Cancer Discovery, 2022;0(0):. 2022 [PMID: 36287038] (IHC, Transgenic Mouse) IHC Transgenic Mouse
R Hashimoto, J Takahashi, K Shirakura, R Funatsu, K Kosugi, S Deguchi, M Yamamoto, Y Tsunoda, M Morita, K Muraoka, M Tanaka, T Kanbara, S Tanaka, S Tamiya, N Tokunoh, A Kawai, M Ikawa, C Ono, K Tachibana, M Kondoh, M Obana, Y Matsuura, A Ohsumi, T Noda, T Yamamoto, Y Yoshioka, YS Torisawa, H Date, Y Fujio, M Nagao, K Takayama, Y Okada SARS-CoV-2 disrupts respiratory vascular barriers by suppressing Claudin-5 expression Science Advances, 2022;8(38):eabo6783. 2022 [PMID: 36129989] (Western Blot, Human) Western Blot Human
M Moulis, SVM Runser, L Glorieux, N Dauguet, C Vanderaa, L Gatto, D Tyteca, P Henriet, FM Spagnoli, D Iber, CE Pierreux Identification and implication of tissue-enriched ligands in epithelial-endothelial crosstalk during pancreas development Scientific Reports, 2022;12(1):12498. 2022 [PMID: 35864120] (IHC, Mouse) IHC Mouse
N Tisch, C Mogler, A Stojanovic, R Luck, EA Korhonen, A Ellerkmann, H Adler, M Singhal, G Schermann, L Erkert, JV Patankar, A Karakatsan, AL Scherr, Y Fuchs, A Cerwenka, S Wirtz, BC Köhler, HG Augustin, C Becker, T Schmidt, C Ruiz de Al Caspase-8 in endothelial cells maintains gut homeostasis and prevents small bowel inflammation in mice Embo Molecular Medicine, 2022;0(0):e14121. 2022 [PMID: 35491615] (IHC, Mouse) IHC Mouse
A Tarafdar, N Wolska, C Krisp, H Schlüter, G Pula The amyloid peptide beta disrupts intercellular junctions and increases endothelial permeability in a NADPH oxidase 1-dependent manner Redox Biology, 2022;52(0):102287. 2022 [PMID: 35358850] (IHC/IF, Mouse) IHC/IF Mouse
CN Duong, R Brückner, M Schmitt, AF Nottebaum, L Braun, MM Zu Brickwe, U Ipe, H Vom Bruch, HR Schöler, G Trapani, B Trappmann, MP Ebrahimkut, S Huveneers, J Rooij, N Ishiyama, M Ikura, D Vestweber Force-induced changes of alpha-catenin conformation stabilize vascular junctions independent of vinculin Journal of Cell Science, 2021;0(0):. 2021 [PMID: 34851405] (ICC, Mouse) ICC Mouse
JM Arpino, H Yin, EK Prescott, SCR Staples, Z Nong, F Li, J Chevalier, B Balint, C O'Neil, R Mortuza, S Milkovich, JJ Lee, D Lorusso, M Sandig, DW Hamilton, DW Holdsworth, TL Poepping, CG Ellis, JG Pickering Low-flow intussusception and metastable VEGFR2 signaling launch angiogenesis in ischemic muscle Science Advances, 2021;7(48):eabg9509. 2021 [PMID: 34826235] (IHC, Mouse) IHC Mouse
S Heino, S Fang, M Lähde, J Högström, S Nassiri, A Campbell, D Flanagan, A Raven, M Hodder, N Nasreddin, HH Xue, M Delorenzi, S Leedham, TV Petrova, O Sansom, K Alitalo Lef1 restricts ectopic crypt formation and tumor cell growth in intestinal adenomas Science Advances, 2021;7(47):eabj0512. 2021 [PMID: 34788095] (IHC, Mouse) IHC Mouse
M Mesnieres, AM Böhm, N Peredo, D Trompet, R Valle-Tenn, M Bajaj, N Corthout, E Nefyodova, R Cardoen, P Baatsen, S Munck, A Nagy, JJ Haigh, S Khurana, CM Verfaillie, C Maes Fetal hematopoietic stem cell homing is controlled by VEGF regulating the integrity and oxidative status of the stromal-vascular bone marrow niches Cell Reports, 2021;36(8):109618. 2021 [PMID: 34433017] (IHC, Mouse) IHC Mouse
S Selbonne, C Madjene, B Salmon, Y Boulaftali, MC Bouton, V Arocas Protease nexin-1 deficiency increases mouse hindlimb neovascularisation following ischemia and accelerates femoral artery perfusion Scientific Reports, 2021;11(1):13412. 2021 [PMID: 34183729] (IHC, Mouse) IHC Mouse
Show All 57 Publications.

Reviews for VE-Cadherin Antibody (AF1002) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using AF1002:
Filter by Applications
Immunofluorescence-frozen sections
(1)
IHC-Fr
(1)
WB
(1)
All Applications
Filter by Species
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
Other VE-Cadherin AF1002
Enlarge
3
reviewed by:
Verified Customer
Immunofluorescence-frozen sections Mouse 04/28/2020
View

Summary

ApplicationOther
Sample TestedEmbryonic brain
SpeciesMouse
LotFQI0117051

Comments

CommentsTissue sections were thawed and blocked in 10% NDS in PBST for 2 hours.
Primary ( 4degrees Overnight ) and secondary (2 hours at RT) were prepared in the same blocking buffer.
Immunohistochemistry-Frozen VE-Cadherin AF1002
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Mouse 03/12/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse
SpeciesMouse
LotFQ10113071
Western Blot VE-Cadherin AF1002
Enlarge
5
reviewed by:
Verified Customer
WB Mouse 02/25/2015
View

Summary

ApplicationWestern Blot
Sample TestedProtein
SpeciesMouse
LotFQ10113071

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Recent Reviews

4.3
5
2
4
0
3
1
2
0
1
0

Verified Customer
04/28/2020
Application: Immunofluorescence-frozen sections
Species: Mouse

Verified Customer
03/12/2015
Application: IHC-Fr
Species: Mouse

Verified Customer
02/25/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol CDH5
Uniprot