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ICAM-1/CD54 Antibody [Unconjugated]

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ICAM-1/CD54 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse ICAM-1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF796) at 15 µg/mL overnight at 4 °C. Tissue was ...read more
Increased stress kinase signaling and JNK pathway-dependent cytokine and chemokine production by primary keratinocytes lacking BRAF and RAF1.(A) Reduced ERK phosphorylation and increased JNK/p38 activation in primary ...read more
Increased stress kinase signaling and JNK pathway-dependent cytokine and chemokine production by primary keratinocytes lacking BRAF and RAF1.(A) Reduced ERK phosphorylation and increased JNK/p38 activation in primary ...read more
Increased stress kinase signaling and JNK pathway-dependent cytokine and chemokine production by primary keratinocytes lacking BRAF and RAF1.(A) Reduced ERK phosphorylation and increased JNK/p38 activation in primary ...read more
D-JNKI1 treatment rescues inflammation in delta / delta ep2 mice.Mice were treated with D-JNKI1 or TAT peptide (22 mg/kg i.p. at 10 days of age) and analyzed after 12 days (A) D-JNKI1 treatment prevents disease onset ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

ICAM-1/CD54 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ICAM-1/CD54
Gln28-Asn485
Accession # Q3U8M7
Specificity
Detects mouse ICAM-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant rat ICAM-1 is observed, approximately 5% cross-reactivity with recombinant human (rh) ICAM-1 and rhICAM-3 is observed, and less than 1% cross-reactivity with recombinant mouse ICAM-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ICAM1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Adhesion Blockade
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 6 Reviews rated 4.5
using
AF796 in the following applications:

Publications
Read Publications using
AF796 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ICAM-1/CD54 Antibody [Unconjugated]

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58

Background

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma , TNF-alpha , IL-1 beta , and LPS. Mouse and human ICAM-1 share approximately 54% amino acid identity.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation.

  1. Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book. Academic Press, p. 74.
  2. Siu, G. et al. (1989) J. Immunol. 143:3813.
  3. Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IHC, B/N

Publications for ICAM-1/CD54 Antibody (AF796)(85)

We have publications tested in 4 confirmed species: Human, Mouse, Hamster, Transgenic Mouse.

We have publications tested in 6 applications: ICC, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.


Filter By Application
ICC
(1)
IHC
(23)
IHC-Fr
(8)
IHC-P
(6)
Neutralization
(1)
Western Blot
(20)
All Applications
Filter By Species
Human
(1)
Mouse
(52)
Hamster
(1)
Transgenic Mouse
(2)
All Species
Showing Publications 1 - 10 of 85. Show All 85 Publications.
Publications using AF796 Applications Species
Manandhar, S;Gaddam, RR;Chambers, S;Bhatia, M; Kupffer Cell Inactivation Alters Endothelial Cell Adhesion Molecules in Cecal Ligation and Puncture-Induced Sepsis Biomolecules 2024-01-09 [PMID: 38254684] (IHC, Mouse) IHC Mouse
Yin, D;Wang, C;Qi, Y;Wang, YC;Hagemann, N;Mohamud Yusuf, A;Dzyubenko, E;Kaltwasser, B;Tertel, T;Giebel, B;Gunzer, M;Popa-Wagner, A;Doeppner, TR;Hermann, DM; Neural precursor cell delivery induces acute post-ischemic cerebroprotection, but fails to promote long-term stroke recovery in hyperlipidemic mice due to mechanisms that include pro-inflammatory responses associated with brain hemorrhages Journal of neuroinflammation 2023-09-15 [PMID: 37715288] (IHC, Mouse) IHC Mouse
Manandhar, S;Chambers, S;Miller, A;Ishii, I;Bhatia, M; Pharmacological Inhibition and Genetic Deletion of Cystathionine Gamma-Lyase in Mice Protects against Organ Injury in Sepsis: A Key Role of Adhesion Molecules on Endothelial Cells International journal of molecular sciences 2023-09-04 [PMID: 37686458] (IHC, Transgenic Mouse, Mouse) IHC Transgenic Mouse, Mouse
Yu, L;Xu, L;Chu, H;Peng, J;Sacharidou, A;Hsieh, HH;Weinstock, A;Khan, S;Ma, L;Durán, JGB;McDonald, J;Nelson, ER;Park, S;McDonnell, DP;Moore, KJ;Huang, LJ;Fisher, EA;Mineo, C;Huang, L;Shaul, PW; Macrophage-to-endothelial cell crosstalk by the cholesterol metabolite 27HC promotes atherosclerosis in male mice Nature communications 2023-07-25 [PMID: 37491347] (IHC, Mouse) IHC Mouse
Anderson R. A. Cerqueira, Leandro Rodrigues, Silvia Abigail Coavoy-Sánchez, Simone A. Teixeira, Karla B. Feitosa, Erika Y. Taniguchi et al. Aedes aegypti salivary gland extract alleviates acute itching by blocking TRPA1 channels Frontiers in Physiology 6/27/2023 [PMID: 37441000]
Cornuault, L;Hérion, FX;Bourguignon, C;Rouault, P;Foussard, N;Alzieu, P;Chapouly, C;Gadeau, AP;Couffinhal, T;Renault, MA; Partial Mural Cell Ablation Disrupts Coronary Vasculature Integrity and Induces Systolic Dysfunction Journal of the American Heart Association 2023-06-22 [PMID: 37345826] (IHC, Western Blot, Transgenic Mouse) IHC, Western Blot Transgenic Mouse
Kim, JY;Yi, MH;Kim, M;Choi, JH;Lee, S;Yong, TS; Production of Dermatophagoides farinae Having Low Bacterial Content Using Ampicillin Journal of immunology research 2023-05-18 [PMID: 37252681] (IHC, Mouse) IHC Mouse
Markos Patsouras, Eirini Alexopoulou, Spyros Foutadakis, Eirini Tsiki, Panagiota Karagianni, Marios Agelopoulos et al. Antiphospholipid antibodies induce proinflammatory and procoagulant pathways in endothelial cells Journal of Translational Autoimmunity 5/2/2023 [PMID: 37216142]
Z Ma, W Li, L Zhuang, T Wen, P Wang, H Yu, Y Liu, Y Yu TMEM59 ablation leads to loss of olfactory sensory neurons and impairs olfactory functions via interaction with inflammation Brain, Behavior, and Immunity, 2023-04-13;111(0):151-168. 2023-04-13 [PMID: 37061103] (IHC, Mouse) IHC Mouse
Hyun-Jin An, Mi-Gyeong Gwon, Hyemin Gu, Seongjae Bae, Jaechan Leem, Jin Bae Lee et al. STAT3/NF‑ kappa B decoy oligodeoxynucleotides inhibit atherosclerosis through regulation of the STAT/NF‑ kappa B signaling pathway in a mouse model of atherosclerosis International Journal of Molecular Medicine 3/31/2023 [PMID: 37026512]
Show All 85 Publications.

Reviews for ICAM-1/CD54 Antibody (AF796) (6) 4.56

Average Rating: 4.5
(Based on 6 reviews)
We have 6 reviews tested in 2 species: Mouse, Other.

Reviews using AF796:
Filter by Applications
IHC-Fr
(1)
IHC
(1)
IF
(3)
WB
(1)
All Applications
Filter by Species
Mouse
(5)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen ICAM-1/CD54 AF796
Enlarge
5
reviewed by:
Chao Xiong
IHC-Fr Mouse 03/14/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Tested4% PFA-PBS fixed frozen sections from murine brain
SpeciesMouse
LotGRG0215121
Immunohistochemistry ICAM-1/CD54 AF796
Enlarge
5
reviewed by:
Verified Customer
IHC Mouse 03/27/2017
View

Summary

ApplicationImmunohistochemistry
Sample Tested4T1 mouse breast cancer cell line,4T1 tumor section
SpeciesMouse
LotGRG0215121

Comments

CommentsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). 4T1 tissues were cut 5 um. After deparaffin steps, the section was pre-treated using heat mediated antigen retrieval with Citrate buffer @ pH 6.0 (C9999, Thermo-Scientific) solution for 20 min at 100 C. Protein blocked was processed with superblock (#37515, Thermo Scientific) for 30 min. Afterwards, the section was then incubated with ICAM-1 pAb with 100X dilution of recommended concentration (100ug/500uL in PBS) for 1h at room temperature. A donkey anti-goat biotinylated secondary antibody (IgG H&L #ab7125, 1/200) was used to detect the primary and visualized using an HRP system (30 min incubation). DAB (#SK-4105, Vector) was used as the chromogen. The section was then counterstained with methyl green (#H-3402, Vector) and mounted with Cytoseal 60.
  5
reviewed by:
Verified Customer
IF Mouse 01/09/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 20592225
SpeciesMouse
  4
reviewed by:
Verified Customer
WB Other 01/09/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22496445
SpeciesOther
  4
reviewed by:
Verified Customer
IF Mouse 01/09/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22661091
SpeciesMouse
  4
reviewed by:
Verified Customer
IF Mouse 01/09/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23232501
SpeciesMouse

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Recent Reviews

4.5
5
3
4
3
3
0
2
0
1
0

Chao Xiong
03/14/2018
Application: IHC-Fr
Species: Mouse

Verified Customer
03/27/2017
Application: IHC
Species: Mouse

 
Verified Customer
01/09/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol ICAM1
Uniprot