CD34 Antibody (MEC 14.7)

Images

 
Immunocytochemistry/ Immunofluorescence: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 antibody was tested in WEHI-3 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse small intestine using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 ...read more
Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 (MEC 14.7) antibody was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).
Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - IHC-P analysis of CD34 on human renal cancer xenograft using CD34 antibody (clone MEC 14.7). The antibody detected the endothelial cells in the ...read more
Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - IHC analysis of a human renal cancer tissue section using CD34 antibody (clone MEC 14.7) at 1:100 dilution. The antibody did not detect human CD34 ...read more
Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human renal cancer xenograft using CD34 antibody clone MEC 14.7 at 1:100 ...read more
Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human renal cancer xenograft using CD34 antibody (clone MEC 14.7) at 1:100 ...read more
Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse colon using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. ...read more
Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse heart using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
MEC 14.7
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1 mg/ml

CD34 Antibody (MEC 14.7) Summary

Immunogen
murine transformed endothelioma cell line t-end.
Localization
Membrane; Single-pass type I membrane protein.
Marker
Hematopoietic Stem Cell Marker
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CD34
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:250
  • ELISA 1:100-1:2000
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100 - 1:250
  • Immunohistochemistry-Frozen 1:100 - 1:250
  • Immunohistochemistry-Paraffin 1:100 - 1:250
  • Immunoprecipitation 1:10-1:500
Application Notes
This CD34 (MEC 14.7) antibody is useful for Immunohistochemistry (on both paraffin-embedded and frozen sections), Flow Cytometry, Immunocytochemistry/Immunofluorescence, Western blot, Immunoprecipitation and ELISA. Antigen retrieval is required for IHC-Paraffin.
Publications
Read Publications using
NB600-1071 in the following applications:

Reactivity Notes

Mouse. Rat reactivity reported in scientific literature (PMID: 28352335). This antibody does not detect Human CD34 and is an execellent tool for marking host derived endothelial cells/vasculature in human cancer xenografts on mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for CD34 Antibody (MEC 14.7)

  • CD34 antigenhematopoietic progenitor cell antigen CD34
  • CD34 molecule
  • CD34
  • HPCA1

Background

CD34 Antibody MEC 14.7 (NB600-1071) is a useful reagent for identification and characterization of capillary endothelial cells. Furthermore, it can be used for isolation and characterization of hematopoietic progenitor cells. In Western blot, the reported molecular mass of CD34 is ranging from 100 to 120 kDa, apart from the HEV form (Sgp90) which is about 90 kDa. MEC14.7 can recognize a band of about 100 kDa from the H5V line but a band of 75 to 85 kDa from normal tissue. Since different patterns of glycosylation of the molecule are seen with different cell types, this might explain the wide range of CD34 molecular mass (from 75-85 kDa to 120 kD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD34 Antibody (NB600-1071)(17)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 5 applications: FLOW, ICC/IF, IHC, IHC-Fr, IHC-P.


Filter By Application
FLOW
(3)
ICC/IF
(3)
IHC
(1)
IHC-Fr
(1)
IHC-P
(6)
All Applications
Filter By Species
Mouse
(8)
Rat
(1)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NB600-1071 Applications Species
Xu B, Li Y, Deng B et al. Chitosan hydrogel improves mesenchymal stem cell transplant survival and cardiac function following myocardial infarction in rats. Exp Ther Med. Feb 1 2017 [PMID: 28352335] (ICC/IF, Rat) ICC/IF Rat
Wei L, Wang H, Yang F et al. Interleukin-17 potently increases non-small cell lung cancer growth. Mol Med Rep 2016 Feb [PMID: 26708832]
Biktasova AK, Dudimah DF, Uzhachenko RV et al. Multivalent forms of the Notch ligand DLL-1 enhance antitumor T cell immunity in lung cancer and improve efficacy of EGFR targeted therapy. Cancer Res. 2015 Sep 24 [PMID: 26404003] (IHC-P, Mouse) IHC-P Mouse
Borriello L, Montes M, Lepelletier Y et al. Structure-based discovery of a small non-peptidic Neuropilins antagonist exerting in vitro and in vivo anti-tumor activity on breast cancer model. Cancer Lett 2014 Jul 28 [PMID: 24752068] (IHC-Fr, Mouse)

Details:
Using the DyLight 488 conjugated version of NB600-1071, catalog number NB600-1071G.
IHC-Fr Mouse
Scheerer N, Dehne N, Stockmann C et al. Myeloid hypoxia-inducible factor-1alpha is essential for skeletal muscle regeneration in mice. J Immunol 2013 Jul 1 [PMID: 23729446] (IHC-P, Mouse) IHC-P Mouse
Savi? R, He X, Fiel I, Schuchman EH. Recombinant Human Acid Sphingomyelinase as an Adjuvant to Sorafenib Treatment of Experimental Liver Cancer. PLoS One 2013 May 28 [PMID: 23724146] (IHC-P, Mouse) IHC-P Mouse
Lee YS, Morinaga H, Kim JJ et al. The Fractalkine/CX3CR1 System Regulates beta Cell Function and Insulin Secretion. Cell 2013 Apr 11 [PMID: 23582329] (IHC-P, Mouse) IHC-P Mouse
Huang YS, Li IH, Chueh SH et al. Mesenchymal stem cells from rat olfactory bulbs can differentiate into cells with cardiomyocyte characteristics. J Tissue Eng Regen Med 2013 Feb 4 [PMID: 23378029]
Branco-Price C, Zhang N, Schnelle M et al. Endothelial Cell HIF-1[alpha] and HIF-2[alpha] Differentially Regulate Metastatic Success. Cancer Cell 1721(1):52-65. 2012 Jan. [PMID: 22264788]
Fiorina P, Pietramaggiori G, Scherer SS et al. The mobilization and effect of endogenous bone marrow progenitor cells in diabetic wound healing. Cell Transplant;19(11):1369-81. 2010 [PMID: 20977829]
Show All 17 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD34 Antibody (NB600-1071). (Showing 1 - 1 of 1 FAQs).

  1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
    • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB600-1071AF405
Alexa Fluor 488 Labeled NB600-1071AF488
Alexa Fluor 647 Labeled NB600-1071AF647
Alexa Fluor 700 Labeled NB600-1071AF700
Allophycocyanin Labeled NB600-1071APC
Biotin Labeled NB600-1071B
DyLight 350 Labeled NB600-1071UV
DyLight 405 Labeled NB600-1071V
DyLight 488 Labeled NB600-1071G
DyLight 550 Labeled NB600-1071R
DyLight 650 Labeled NB600-1071C
DyLight 680 Labeled NB600-1071FR
DyLight 755 Labeled NB600-1071IR
FITC Labeled NB600-1071F
HRP Labeled NB600-1071H
PE Labeled NB600-1071PE
PerCP Labeled NB600-1071PCP

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Blogs on CD34.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

CD68 (Cluster of differentiation 68, GP110, LAMP4, SCARD1)
CD68 belongs to a growing family of hematopoietic mucin-like molecules known as lysosomal/endosomal-associated membrane glycoproteins (LAMPs). Other LAMP family members included leukosialin, stem cell antigen CD34, and GlyCAM-1. CD68 encodes a 110-kD...  Read full blog post.

CD34 (Cluster of differentiation 34, hematopoietic progenitor cell antigen)
CD34 is a cell-surface glycoprotein type 1 transmembrane protein that belongs to the sialomucin family. CD34 comprises of an intracellular cytoplasmic domain with consensus sites for serine, threonine, tyrosine and active protein kinase C (PKC). Becau...  Read full blog post.

CD34 Serves as an Important Marker in Disease Research
CD34 is a membrane protein that aids cells in cell-cell adhesion. Although little is known about its function, CD34 is an important marker for hematopoietic stem cells (HSCs), muscle satellite cells, and endothelial cells. HSCs can be found in bone ma...  Read full blog post.

CD34 Serves as an Important Marker in Disease Research
CD34 is a cell surface glycoprotein that aids cells in cell-cell adhesion. It is expressed on endothelial cells where it is known to bind L-selectin and may aid in migration of T-cells. Moreover, it is expressed on hematopoietic stem cells (HSC), musc...  Read full blog post.

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Bioinformatics

Gene Symbol CD34
Entrez