CD34 Antibody (MEC 14.7)


Immunocytochemistry/ Immunofluorescence: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 antibody was tested in WEHI-3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and more
Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse small intestine using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed more
Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 (MEC 14.7) antibody was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).
Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of CD34 on human renal cancer xenograft using CD34 antibody (clone MEC 14.7). The antibody detected the endothelial cells in the tumor more
Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a human renal cancer tissue section using CD34 antibody (clone MEC 14.7) at 1:100 dilution. The antibody did not detect human CD34 which more
Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of human renal cancer xenograft using CD34 antibody clone MEC 14.7 at 1:100 dilution. The signal was developed more
Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of human renal cancer xenograft using CD34 antibody (clone MEC 14.7) at 1:100 dilution. The staining was detected more
Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse colon using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using more
Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse heart using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using more

Product Details

Reactivity Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
MEC 14.7
1 mg/ml

Order Details

CD34 Antibody (MEC 14.7) Summary

Murine transformed endothelioma cell line t-end.
Membrane; Single-pass type I membrane protein.
Hematopoietic Stem Cell Marker
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:250
  • ELISA 1:100-1:2000
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100 - 1:250
  • Immunohistochemistry-Frozen 1:100 - 1:250
  • Immunohistochemistry-Paraffin 1:100 - 1:250
  • Immunoprecipitation 1:10-1:500
Application Notes
This CD34 (MEC 14.7) antibody is useful for Immunohistochemistry (on both paraffin-embedded and frozen sections), Flow Cytometry, Immunocytochemistry/Immunofluorescence, Western blot, Immunoprecipitation and ELISA. Antigen retrieval is required for IHC-Paraffin.
Read Publications using
NB600-1071 in the following applications:

Reactivity Notes

This antibody does not detect Human CD34 and is an execellent tool for marking host derived endothelial cells/vasculature in human cancer xenografts on mouse. Rat reactivity reported in scientific literature (PMID: 28352335).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
1 mg/ml
Protein G purified

Alternate Names for CD34 Antibody (MEC 14.7)

  • CD34 antigenhematopoietic progenitor cell antigen CD34
  • CD34 molecule
  • CD34
  • HPCA1


CD34 Antibody MEC 14.7 (NB600-1071) is a useful reagent for identification and characterization of capillary endothelial cells. Furthermore, it can be used for isolation and characterization of hematopoietic progenitor cells. In Western blot, the reported molecular mass of CD34 is ranging from 100 to 120 kDa, apart from the HEV form (Sgp90) which is about 90 kDa. MEC14.7 can recognize a band of about 100 kDa from the H5V line but a band of 75 to 85 kDa from normal tissue. Since different patterns of glycosylation of the molecule are seen with different cell types, this might explain the wide range of CD34 molecular mass (from 75-85 kDa to 120 kD).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD34 Antibody (NB600-1071)(19)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 6 applications: FLOW, Flow, ICC/IF, IHC, IHC-Fr, IHC-P.

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Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NB600-1071 Applications Species
Kolb R, Kluz P, Tan ZW et al. Obesity-associated inflammation promotes angiogenesis and breast cancer via angiopoietin-like 4 Oncogene Dec 5 2018 [PMID: 30518876] (IHC-Fr, Mouse) IHC-Fr Mouse
Sakurai R, Liu J, Wang Y et al. Prevention of Perinatal Nicotine-induced Bone Marrow Mesenchymal Stem Cell Myofibroblast Differentiation by Augmenting the Lipofibroblast Phenotype. Clin. Sci. Oct 11 2018 [PMID: 30309879] (Flow, Rat) Flow Rat
Xu B, Li Y, Deng B et al. Chitosan hydrogel improves mesenchymal stem cell transplant survival and cardiac function following myocardial infarction in rats. Exp Ther Med. Feb 1 2017 [PMID: 28352335] (ICC/IF, Rat) ICC/IF Rat
Wei L, Wang H, Yang F et al. Interleukin-17 potently increases non-small cell lung cancer growth. Mol Med Rep 2016 Feb [PMID: 26708832]
Biktasova AK, Dudimah DF, Uzhachenko RV et al. Multivalent forms of the Notch ligand DLL-1 enhance antitumor T cell immunity in lung cancer and improve efficacy of EGFR targeted therapy. Cancer Res. 2015 Sep 24 [PMID: 26404003] (IHC-P, Mouse) IHC-P Mouse
Borriello L, Montes M, Lepelletier Y et al. Structure-based discovery of a small non-peptidic Neuropilins antagonist exerting in vitro and in vivo anti-tumor activity on breast cancer model. Cancer Lett 2014 Jul 28 [PMID: 24752068] (IHC-Fr, Mouse)

Using the DyLight 488 conjugated version of NB600-1071, catalog number NB600-1071G.
IHC-Fr Mouse
Scheerer N, Dehne N, Stockmann C et al. Myeloid hypoxia-inducible factor-1alpha is essential for skeletal muscle regeneration in mice. J Immunol 2013 Jul 1 [PMID: 23729446] (IHC-P, Mouse) IHC-P Mouse
Savi? R, He X, Fiel I, Schuchman EH. Recombinant Human Acid Sphingomyelinase as an Adjuvant to Sorafenib Treatment of Experimental Liver Cancer. PLoS One 2013 May 28 [PMID: 23724146] (IHC-P, Mouse) IHC-P Mouse
Lee YS, Morinaga H, Kim JJ et al. The Fractalkine/CX3CR1 System Regulates beta Cell Function and Insulin Secretion. Cell 2013 Apr 11 [PMID: 23582329] (IHC-P, Mouse) IHC-P Mouse
Huang YS, Li IH, Chueh SH et al. Mesenchymal stem cells from rat olfactory bulbs can differentiate into cells with cardiomyocyte characteristics. J Tissue Eng Regen Med 2013 Feb 4 [PMID: 23378029]
Show All 19 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD34 Antibody (NB600-1071). (Showing 1 - 1 of 1 FAQs).

  1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
    • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-1071AF350
Alexa Fluor 405 NB600-1071AF405
Alexa Fluor 488 NB600-1071AF488
Alexa Fluor 532 NB600-1071AF532
Alexa Fluor 594 NB600-1071AF594
Alexa Fluor 647 NB600-1071AF647
Alexa Fluor 700 NB600-1071AF700
Alexa Fluor 750 NB600-1071AF750
Allophycocyanin NB600-1071APC
Allophycocyanin/Cy7 NB600-1071APCCY7
Biotin NB600-1071B
DyLight 350 NB600-1071UV
DyLight 405 NB600-1071V
DyLight 488 NB600-1071G
DyLight 550 NB600-1071R
DyLight 594 NB600-1071DL594
DyLight 650 NB600-1071C
DyLight 680 NB600-1071FR
DyLight 755 NB600-1071IR
FITC NB600-1071F
HRP NB600-1071H
Janelia Fluor 549 NB600-1071JF549
Janelia Fluor 646 NB600-1071JF646
PE NB600-1071PE
PE/Atto594 NB600-1071PEATT594
PE/Cy5.5 NB600-1071PECY55
PE/Cy7 NB600-1071PECY7
PerCP NB600-1071PCP

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The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

CD68 (Cluster of differentiation 68, GP110, LAMP4, SCARD1)
CD68 belongs to a growing family of hematopoietic mucin-like molecules known as lysosomal/endosomal-associated membrane glycoproteins (LAMPs). Other LAMP family members included leukosialin, stem cell antigen CD34, and GlyCAM-1. CD68 encodes a 110-kD...  Read full blog post.

CD34 (Cluster of differentiation 34, hematopoietic progenitor cell antigen)
CD34 is a cell-surface glycoprotein type 1 transmembrane protein that belongs to the sialomucin family. CD34 comprises of an intracellular cytoplasmic domain with consensus sites for serine, threonine, tyrosine and active protein kinase C (PKC). Becau...  Read full blog post.

CD34 Serves as an Important Marker in Disease Research
CD34 is a membrane protein that aids cells in cell-cell adhesion. Although little is known about its function, CD34 is an important marker for hematopoietic stem cells (HSCs), muscle satellite cells, and endothelial cells. HSCs can be found in bone ma...  Read full blog post.

CD34 Serves as an Important Marker in Disease Research
CD34 is a cell surface glycoprotein that aids cells in cell-cell adhesion. It is expressed on endothelial cells where it is known to bind L-selectin and may aid in migration of T-cells. Moreover, it is expressed on hematopoietic stem cells (HSC), musc...  Read full blog post.

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Gene Symbol CD34