Recombinant Human P-Selectin/CD62P Fc Chimera Protein, CF Summary
Details of Functionality |
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. P-Selectin/Fc Chimera, immobilized at 10 µg/mL, will induce greater than 50% adhesion on U937 cells (100 µL/well at 1 x 10 6 cells/mL). Optimal dilutions should be determined be each laboratory for each application. |
Source |
Mouse myeloma cell line, NS0-derived human P-Selectin/CD62P protein Human P-Selectin (Trp42 - Ala771) Accession # P16109 | IEGRDMD | Human IgG 1 (Pro100 - Lys330) | N-terminus | | C-terminus | |
Accession # |
|
N-terminal Sequence |
Trp42 |
Structure / Form |
Disulfide-linked homodimer |
Protein/Peptide Type |
Recombinant Proteins |
Gene |
SELP |
Purity |
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
Endotoxin Note |
<0.01 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
106 kDa (monomer). Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE |
146-160 kDa, reducing conditions |
Publications |
Read Publications using 137-PS in the following applications:
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Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution. |
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS. |
Purity |
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
Reconstitution Instructions |
Reconstitute at 100 μg/mL in sterile PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human P-Selectin/CD62P Fc Chimera Protein, CF
Background
Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin glycoprotein ligand-1). P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L-Selectin, P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling’ of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. Human P-Selectin cDNA encodes an 830 amino acid (aa) residue type I transmembrane protein with a 41 aa signal peptide, a 730 aa extracellular domain, a transmembrane domain and a short (35 aa) cytoplasmic domain. The extracellular domain has an NH 2 -terminal C-type lectin domain and an EGF-like domain followed by a series of complement factor A repeat homology domains. The extracellular domains of human and mouse P-Selectin share approximately 73% sequence homology.
- Kansas, G.S. (1996) Blood 88:3259.
- McEver, R.P. and R.D. Cummings (1997) J. Clin. Invest. 100:485.
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