Recombinant Human P-Selectin/CD62P Fc Chimera Protein, CF

Images

 
There are currently no images for P-Selectin/CD62P (137-PS).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human P-Selectin/CD62P Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. P-Selectin/Fc Chimera, immobilized at 10 µg/mL, will induce greater than 50% adhesion on U937 cells (100 µL/well at 1 x 106 cells/mL).
Optimal dilutions should be determined be each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human P-Selectin/CD62P protein
Human P-Selectin
(Trp42 - Ala771)
Accession # P16109
IEGRDMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Trp42
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
SELP
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
106 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
146-160 kDa, reducing conditions
Publications
Read Publications using
137-PS in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human P-Selectin/CD62P Fc Chimera Protein, CF

  • CD62P antigen
  • CD62P
  • FLJ45155
  • GMP140
  • GRMP
  • PADGEM
  • PADGEMantigen CD62)
  • PSEL
  • P-Selectin
  • selectin P (granule membrane protein 140kDa, antigen CD62)
  • SELP

Background

Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin glycoprotein ligand-1).

P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L-Selectin, P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling’ of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

Human P-Selectin cDNA encodes an 830 amino acid (aa) residue type I transmembrane protein with a 41 aa signal peptide, a 730 aa extracellular domain, a transmembrane domain and a short (35 aa) cytoplasmic domain. The extracellular domain has an NH2-terminal C-type lectin domain and an EGF-like domain followed by a series of complement factor A repeat homology domains. The extracellular domains of human and mouse P-Selectin share approximately 73% sequence homology.

  1. Kansas, G.S. (1996) Blood 88:3259.
  2. McEver, R.P. and R.D. Cummings (1997) J. Clin. Invest. 100:485.

Customers Who Viewed This Item Also Viewed...

BBA16
Species: Hu
Applications: WB, IHC, IP, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
796-IC
Species: Mu
NB600-586
Species: Hu
Applications: ICC/IF, IHC, IHC-P
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NBP1-84550
Species: Hu
Applications: WB, IHC, IHC-P
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP2-42225
Species: Hu, Ca
Applications: WB, DB, EM, ELISA, Flow, ICC/IF, IP, Flow-IC
NBP2-37384
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P
AF1730
Species: Hu
Applications: WB, Flow, AdBlk, CyTOF-ready, ICC
NB100-78039
Species: Hu, Mu
Applications: WB, ELISA, Flow, IHC, IHC-Fr, IHC-P, CyTOF-ready, Flow-CS
NB110-89474
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Single Cell Western
NBP2-14934
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF3628
Species: Mu, Rt
Applications: WB, Flow, IHC, CyTOF-ready, ICC
137-PS
Species: Hu
Applications: Bioactivity

Publications for P-Selectin/CD62P (137-PS)(27)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 7 applications: Binding Assay, Bioassay, ELISA Standard, ELISA capture, Flow, ICC, Surface Plasmon Resonance.


Filter By Application
Binding Assay
(1)
Bioassay
(16)
ELISA Standard
(1)
ELISA capture
(1)
Flow
(6)
ICC
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(23)
Mouse
(2)
N/A
(1)
All Species
Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using 137-PS Applications Species
CH Lee, HH Zhang, SP Singh, L Koo, J Kabat, H Tsang, TP Singh, JM Farber C/EBP? drives interactions between human MAIT cells and endothelial cells that are important for extravasation Elife, 2018;7(0):. 2018 [PMID: 29469805] (Bioassay, Human) Bioassay Human
FW Lam, Q Da, B Guillory, MA Cruz Recombinant Human Vimentin Binds to P-Selectin and Blocks Neutrophil Capture and Rolling on Platelets and Endothelium J. Immunol., 2018;0(0):. 2018 [PMID: 29335256] (Bioassay, Human) Bioassay Human
Y Seong, NH Lazarus, L Sutherland, A Habtezion, T Abramson, XS He, HB Greenberg, EC Butcher Trafficking receptor signatures define blood plasmablasts responding to tissue-specific immune challenge JCI Insight, 2017;2(6):e90233. 2017 [PMID: 28352656] (Flow, Human) Flow Human
IT Li, T Ha, YR Chemla Mapping cell surface adhesion by rotation tracking and adhesion footprinting Sci Rep, 2017;7(0):44502. 2017 [PMID: 28290531] (Bioassay, Human) Bioassay Human
EE Edwards, SN Thomas P-Selectin and ICAM-1 synergy in mediating THP-1 monocyte adhesion in hemodynamic flow is length dependent Integr Biol (Camb), 2017;9(4):313-327. 2017 [PMID: 28262902] (Bioassay, Human) Bioassay Human
Bo Li Development of Polymer Microcapsules Functionalized with Fucoidan to Target P-Selectin Overexpressed in Cardiovascular Diseases Adv Healthc Mater, 2016;0(0):. 2016 [PMID: 27943662] (Bioassay) Bioassay
Simon N Robinson Non-fucosylated CB CD34(+) cells represent a good target for enforced fucosylation to improve engraftment following cord blood transplantation Cytotherapy, 2016;0(0):. 2016 [PMID: 27919572] (Bioassay, Human) Bioassay Human
Using CRISPR-Cas9 to quantify the contributions of O-glycans, N-glycans and Glycosphingolipids to human leukocyte-endothelium adhesion Sci Rep, 2016;6(0):30392. 2016 [PMID: 27458028] (Bioassay, Human) Bioassay Human
Krishnamurthy V, Sardar M, Ying Y, Song X, Haller C, Dai E, Wang X, Hanjaya-Putra D, Sun L, Morikis V, Simon S, Woods R, Cummings R, Chaikof E Glycopeptide analogues of PSGL-1 inhibit P-selectin in vitro and in vivo. Nat Commun, 2015;6(0):6387. 2015 [PMID: 25824568] (Bioassay, Human) Bioassay Human
Xu T, Liu W, Yang C, Ba X, Wang X, Jiang Y, Zeng X Lipid raft-associated beta-adducin is required for PSGL-1-mediated neutrophil rolling on P-selectin. J Leukoc Biol, 2015;97(2):297-306. 2015 [PMID: 25425738] (Bioassay, Human) Bioassay Human
Show All 27 Publications.

Reviews for P-Selectin/CD62P (137-PS) (0)

There are no reviews for P-Selectin/CD62P (137-PS). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for P-Selectin/CD62P (137-PS) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for P-Selectin/CD62P (137-PS)

Discover related pathways, diseases and genes to P-Selectin/CD62P (137-PS). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for P-Selectin/CD62P (137-PS)

Discover more about diseases related to P-Selectin/CD62P (137-PS).
 

Pathways for P-Selectin/CD62P (137-PS)

View related products by pathway.

PTMs for P-Selectin/CD62P (137-PS)

Learn more about PTMs related to P-Selectin/CD62P (137-PS).

Blogs on P-Selectin/CD62P

There are no specific blogs for P-Selectin/CD62P, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human P-Selectin/CD62P Fc Chimera Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol SELP
Entrez
Uniprot