Recombinant Human P-Selectin/CD62P Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human P-Selectin/CD62P Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. P-Selectin/Fc Chimera, immobilized at 10 µg/mL, will induce greater than 50% adhesion on U937 cells (100 µL/well at 1 x 10⁶ cells/mL).
  Optimal dilutions should be determined be each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived human P-Selectin/CD62P protein
  

  Human P-Selectin (Trp42 - Ala771) Accession # P16109	IEGRDMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: P16109
• N-terminal Sequence: Trp42
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: SELP
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 106 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 146-160 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human P-Selectin/CD62P Fc Chimera Protein, CF

• CD62P antigen
• CD62P
• FLJ45155
• GMP140
• GRMP
• PADGEM
• PADGEMantigen CD62)
• PSEL
• P-Selectin
• selectin P (granule membrane protein 140kDa, antigen CD62)
• SELP

Background

Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin glycoprotein ligand-1).

P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L-Selectin, P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling’ of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

Human P-Selectin cDNA encodes an 830 amino acid (aa) residue type I transmembrane protein with a 41 aa signal peptide, a 730 aa extracellular domain, a transmembrane domain and a short (35 aa) cytoplasmic domain. The extracellular domain has an NH₂-terminal C-type lectin domain and an EGF-like domain followed by a series of complement factor A repeat homology domains. The extracellular domains of human and mouse P-Selectin share approximately 73% sequence homology.

1. Kansas, G.S. (1996) Blood 88:3259.
2. McEver, R.P. and R.D. Cummings (1997) J. Clin. Invest. 100:485.

Publications for P-Selectin/CD62P (137-PS)(41)

Publications using 137-PS

• K Taverner, Y Murad, AB Yasunaga, C Furrer, J Little, ITS Li The effect of type-2 diabetes conditions on neutrophil rolling adhesion Bmc Research Notes, 2022;15(1):355. 2022 [PMID: 36463286] (Bioassay, Human)
• S Wu, J Liu, Y Zhang, J Song, Z Zhang, Y Yang, M Wu, H Tong Structural characterization and antagonistic effect against P-selectin-mediated function of SFF-32, a fucoidan fraction from Sargassum fusiforme Journal of ethnopharmacology, 2022;295(0):115408. 2022 [PMID: 35659565] (Bioassay, Human)
• L Wen, A Marki, Z Wang, M Orecchioni, J Makings, M Billitti, E Wang, SSA Suthahar, K Kim, WB Kiosses, Z Mikulski, K Ley A humanized beta2 integrin knockin mouse reveals localized intra- and extravascular neutrophil integrin activation in�vivo Cell Reports, 2022;39(9):110876. 2022 [PMID: 35649374] (Bioassay, Human)
• SU Khan, Y Xia, D Goodale, G Schoettle, AL Allan Lung-Derived Selectins Enhance Metastatic Behavior of Triple Negative Breast Cancer Cells Biomedicines, 2021;9(11):. 2021 [PMID: 34829810] (Bioassay, Human)
• W Liu, AY Hsu, Y Wang, T Lin, H Sun, JS Pachter, A Groisman, M Imperioli, FW Yungher, L Hu, P Wang, Q Deng, Z Fan Mitofusin-2 regulates leukocyte adhesion and beta2 integrin activation Journal of leukocyte biology, 2021;0(0):. 2021 [PMID: 34494308] (Bioassay, Human)
• AB Yasunaga, ITS Li Quantification of fast molecular adhesion by fluorescence footprinting Science Advances, 2021;7(34):. 2021 [PMID: 34407937] (Bioassay, Human)
• FW Lam, CA Brown, C Valladolid, DC Emebo, TG Palzkill, MA Cruz The vimentin rod domain blocks P-selectin-P-selectin glycoprotein ligand 1 interactions to attenuate leukocyte adhesion to inflamed endothelium PLoS ONE, 2020;15(10):e0240164. 2020 [PMID: 33048962] (Bioassay, N/A)
• U Valentiner, J Knips, R Pries, T Clauditz, A Münscher, G Sauter, B Wollenberg, U Schumacher Selectin Binding Sites Are Involved in Cell Adhesive Properties of Head and Neck Squamous Cell Carcinoma Cancers (Basel), 2019;11(11):. 2019 [PMID: 31661833] (Bioassay, Human)
• A Kelkar, Y Zhu, T Groth, G Stolfa, AB Stablewski, N Singhi, M Nemeth, S Neelamegha Doxycycline-Dependent Self-Inactivation of CRISPR-Cas9 to Temporally Regulate On- and Off-Target Editing Mol. Ther., 2019;0(0):. 2019 [PMID: 31601489] (Flow Cytometry, Human)
• W Fu, C Lei, S Liu, Y Cui, C Wang, K Qian, T Li, Y Shen, X Fan, F Lin, M Ding, M Pan, X Ye, Y Yang, S Hu CAR exosomes derived from effector CAR-T cells have potent antitumour effects and low toxicity Nat Commun, 2019;10(1):4355. 2019 [PMID: 31554797] (ELISA Control, Human)
• H Sun, Z Fan, AR Gingras, MA Lopez-Rami, MH Ginsberg, K Ley Frontline Science: A flexible kink in the transmembrane domain impairs β2 integrin extension and cell arrest from rolling J. Leukoc. Biol., 2019;0(0):. 2019 [PMID: 31475386] (Bioassay, Human)
• EW Martin, R Malgor, VA Resto, DJ Goetz, MM Burdick Dynamic biochemical tissue analysis detects functional selectin ligands on human cancer tissues Sci Rep, 2019;9(1):8511. 2019 [PMID: 31186472] (Bioassay, Human)
• JF Osborn, SJ Hobbs, JL Mooster, TN Khan, AM Kilgore, JC Harbour, JC Nolz Central memory CD8+ T cells become CD69+ tissue-residents during viral skin infection independent of CD62L-mediated lymph node surveillance PLoS Pathog., 2019;15(3):e1007633. 2019 [PMID: 30875408] (Binding Assay, Human)
• K Kitamura, K Sato, M Sawabe, M Yoshida, N Hagiwara P-Selectin Glycoprotein Ligand-1 (PSGL-1) Expressing CD4 T Cells Contribute Plaque Instability in Acute Coronary Syndrome Circ. J., 2018;0(0):. 2018 [PMID: 29962384] (Human)
• CH Lee, HH Zhang, SP Singh, L Koo, J Kabat, H Tsang, TP Singh, JM Farber C/EBP? drives interactions between human MAIT cells and endothelial cells that are important for extravasation Elife, 2018;7(0):. 2018 [PMID: 29469805] (Bioassay, Human)
• FW Lam, Q Da, B Guillory, MA Cruz Recombinant Human Vimentin Binds to P-Selectin and Blocks Neutrophil Capture and Rolling on Platelets and Endothelium J. Immunol., 2018;0(0):. 2018 [PMID: 29335256] (Bioassay, Human)
• Y Seong, NH Lazarus, L Sutherland, A Habtezion, T Abramson, XS He, HB Greenberg, EC Butcher Trafficking receptor signatures define blood plasmablasts responding to tissue-specific immune challenge JCI Insight, 2017;2(6):e90233. 2017 [PMID: 28352656] (Flow Cytometry, Human)
• IT Li, T Ha, YR Chemla Mapping cell surface adhesion by rotation tracking and adhesion footprinting Sci Rep, 2017;7(0):44502. 2017 [PMID: 28290531] (Bioassay, Human)
• EE Edwards, SN Thomas P-Selectin and ICAM-1 synergy in mediating THP-1 monocyte adhesion in hemodynamic flow is length dependent Integr Biol (Camb), 2017;9(4):313-327. 2017 [PMID: 28262902] (Bioassay, Human)
• Bo Li Development of Polymer Microcapsules Functionalized with Fucoidan to Target P-Selectin Overexpressed in Cardiovascular Diseases Adv Healthc Mater, 2016;0(0):. 2016 [PMID: 27943662] (Bioassay)
• Simon N Robinson Non-fucosylated CB CD34(+) cells represent a good target for enforced fucosylation to improve engraftment following cord blood transplantation Cytotherapy, 2016;0(0):. 2016 [PMID: 27919572] (Bioassay, Human)
• Using CRISPR-Cas9 to quantify the contributions of O-glycans, N-glycans and Glycosphingolipids to human leukocyte-endothelium adhesion Sci Rep, 2016;6(0):30392. 2016 [PMID: 27458028] (Bioassay, Human)
• Krishnamurthy V, Sardar M, Ying Y, Song X, Haller C, Dai E, Wang X, Hanjaya-Putra D, Sun L, Morikis V, Simon S, Woods R, Cummings R, Chaikof E Glycopeptide analogues of PSGL-1 inhibit P-selectin in vitro and in vivo. Nat Commun, 2015;6(0):6387. 2015 [PMID: 25824568] (Bioassay, Human)
• Xu T, Liu W, Yang C, Ba X, Wang X, Jiang Y, Zeng X Lipid raft-associated beta-adducin is required for PSGL-1-mediated neutrophil rolling on P-selectin. J Leukoc Biol, 2015;97(2):297-306. 2015 [PMID: 25425738] (Bioassay, Human)
• Francischetti I, Ma D, Andersen J, Ribeiro J Evidence for a lectin specific for sulfated glycans in the salivary gland of the malaria vector, Anopheles gambiae. PLoS ONE, 2014;9(9):e107295. 2014 [PMID: 25207644] (Bioassay, Human)
• Heidemann F, Schildt A, Schmid K, Bruns O, Riecken K, Jung C, Ittrich H, Wicklein D, Reimer R, Fehse B, Heeren J, Luers G, Schumacher U, Heine M Selectins mediate small cell lung cancer systemic metastasis. PLoS ONE, 2014;9(4):e92327. 2014 [PMID: 24699516] (Flow Cytometry, Human)
• Nolz J, Harty J IL-15 regulates memory CD8+ T cell O-glycan synthesis and affects trafficking. J Clin Invest, 2014;124(3):1013-26. 2014 [PMID: 24509081] (Flow Cytometry, Human)
• Domingos-Pereira S, Decrausaz L, Derre L, Bobst M, Romero P, Schiller J, Jichlinski P, Nardelli-Haefliger D Intravaginal TLR agonists increase local vaccine-specific CD8 T cells and human papillomavirus-associated genital-tumor regression in mice. Mucosal Immunol, 2013;6(2):393-404. 2013 [PMID: 22968420] (Bioassay, Mouse)
• Rao NV, Argyle B, Xu X, Reynolds PR, Walenga JM, Prechel M, Prestwich GD, MacArthur RB, Walters BB, Hoidal JR, Kennedy TP Low anticoagulant heparin targets multiple sites of inflammation, suppresses heparin-induced thrombocytopenia, and inhibits interaction of RAGE with its ligands. Am. J. Physiol., Cell Physiol., 2010;299(1):C97-110. 2010 [PMID: 20375277] (ELISA (Capture), Human)
• Ni Z, Walcheck B Cutaneous lymphocyte-associated antigen (CLA) T cells up-regulate P-selectin ligand expression upon their activation. Clin. Immunol., 2009;133(2):257-64. 2009 [PMID: 19665434] (Flow Cytometry, Human)
• Kato N, Yuzawa Y, Kosugi T The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion. J. Am. Soc. Nephrol., 2009;20(7):1565-76. 2009 [PMID: 19443639] (Binding Assay, Mouse)
• Kerr SC, Fieger CB, Snapp KR, Rosen SD Endoglycan, a member of the CD34 family of sialomucins, is a ligand for the vascular selectins. J. Immunol., 2008;181(2):1480-90. 2008 [PMID: 18606703] (Bioassay, Human)
• Nonomura C, Kikuchi J, Kiyokawa N, Ozaki H, Mitsunaga K, Ando H, Kanamori A, Kannagi R, Fujimoto J, Muroi K, Furukawa Y, Nakamura M CD43, but not P-selectin glycoprotein ligand-1, functions as an E-selectin counter-receptor in human pre-B-cell leukemia NALL-1. Cancer Res., 2008;68(3):790-9. 2008 [PMID: 18245480] (Flow Cytometry, Human)
• Simonis D, Fritzsche J, Alban S, Bendas G Kinetic analysis of heparin and glucan sulfates binding to P-selectin and its impact on the general understanding of selectin inhibition. Biochemistry, 2007;46(20):6156-64. 2007 [PMID: 17458940] (Surface Plasmon Resonance, Human)
• Choudhury A, Chung I, Blann AD, Lip GY Elevated platelet microparticle levels in nonvalvular atrial fibrillation: relationship to p-selectin and antithrombotic therapy. Chest, 2007;131(3):809-15. 2007 [PMID: 17356097] (ELISA (Standard), N/A)
• Ni&lt;/LastName&gt;&lt;ForeName&gt;Zh Z&lt;/Initial, Ni Z, Walcheck B Varied levels of reactivity by different E-selectin/Fc constructs with cutaneous lymphocyte-associated antigen (CLA)(+) CD4(+) T cells. Immunol. Lett., 2007;108(2):179-82. 2007 [PMID: 17222460] (Bioassay, Human)
• Monzavi-Karbassi B, Stanley JS, Hennings L, Jousheghany F, Artaud C, Shaaf S, Kieber-Emmons T Chondroitin sulfate glycosaminoglycans as major P-selectin ligands on metastatic breast cancer cell lines. Int. J. Cancer, 2007;120(6):1179-91. 2007 [PMID: 17154173] (Bioassay, Human)
• Bestebroer J, Poppelier MJ, Ulfman LH, Lenting PJ, Denis CV, van Kessel KP, van Strijp JA, de Haas CJ Staphylococcal superantigen-like 5 binds PSGL-1 and inhibits P-selectin-mediated neutrophil rolling. Blood, 2007;109(7):2936-43. 2007 [PMID: 17132726] (ICC, Human)
• Ni Z, Campbell JJ, Niehans G, Walcheck B The monoclonal antibody CHO-131 identifies a subset of cutaneous lymphocyte-associated antigen T cells enriched in P-selectin-binding cells. J. Immunol., 2006;177(7):4742-8. 2006 [PMID: 16982914] (Flow Cytometry, Human)
• Hirahara K, Liu L, Clark RA, Yamanaka K, Fuhlbrigge RC, Kupper TS The majority of human peripheral blood CD4+CD25highFoxp3+ regulatory T cells bear functional skin-homing receptors. J. Immunol., 2006;177(7):4488-94. 2006 [PMID: 16982885] (Bioassay, Human)
• Gao Y, Li N, Fei R, Chen Z, Zheng S, Zeng X P-Selectin-mediated acute inflammation can be blocked by chemically modified heparin, RO-heparin. Mol. Cells, 2005;19(3):350-5. 2005 [PMID: 15995351] (Bioassay, Human)

Bioinformatics

• Gene Symbol: SELP
• Uniprot:
  • P16109()