Western Blot: Factor VIII Antibody [NB100-91761] - Detection of Factor VIII protein in extracts from human umbilical vein endothelial cells (HUVECs).
Immunocytochemistry/ Immunofluorescence: Factor VIII Antibody [NB100-91761] - Factor VIII antibody was tested 1:50 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) ...read more
Immunohistochemistry-Paraffin: Factor VIII Antibody [NB100-91761] - Staining of Factor VIII in a formalin fixed and paraffin embedded tissue section of mouse bladder. The primary antibody was used at 1:100 dilution and ...read more
Western Blot: Factor VIII Antibody [NB100-91761] - Analysis of human placenta tissue lysate using Factor VIII antibody at 1:500.
Western Blot: Factor VIII Antibody [NB100-91761] - Analysis of HepG2 whole cell lysate (lane 1), Mouse liver tissue lysate (lane 2), and Rat liver tissue lysate (lane 3) using Factor VIII antibody at 1:500 with ECL ...read more
Use in ELISA was reported in scientific literature.
267 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Immunogen affinity purified
Alternate Names for Factor VIII Antibody
coagulation factor VIII, procoagulant component
coagulation factor VIIIc
F8Ccoagulation factor VIII
factor VIII F8B
Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (prothrombin, and Factors X, IX, V, and VIII), which are involved in a blood coagulation cascade that leads to the formation of insoluble fibrin clots and the promotion of platelet aggregation. Coagulation factor VIII is a 2351 amino acid, non-covalent heterodimer, that circulates as an inactive procofactor. When catalyzed by thrombin, factor VIII is converted to active Factor VIIIa, which associates with factor IXa. This complex catalyzes the conversion of factor X to factor Xa. This results in the amplification phase of coagulation. [PMID: 16513527]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Factor VIII Antibody (NB100-91761). (Showing 1 - 2 of 2 FAQs).
I am interested in knowing whether your antibody (NB100-91761) can neutralize FVIII in rats.
Unfortunately, our product NB100-91761 has not yet been tested for blocking or neutralizing assays. We therefore do not have any data to support or deny this function.
There is a western blot image in the product description testing human factor VIII in HepG2 whole cell lysate. I want to know whether the HepG2 used were untransfected WT cells or transfected by an FVIII-coding plasmid?
The Western blot data on our product page for NB100-91761 was generated from HepG2 whole cell lysate, mouse liver tissue lysate and rat liver tissue lysate. The HepG2 whole cell lysate was derived from endogenous cells; these had not been transfected to over-express the protein.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.