Factor VIII Antibody

Images

 
Western Blot: Factor VIII Antibody [NB100-91761] - WB detection of Factor VIII protein in extracts from human umbilical vein endothelial cells (HUVECs).
Immunocytochemistry/ Immunofluorescence: Factor VIII Antibody [NB100-91761] - Factor VIII antibody was tested 1:50 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) ...read more
Immunohistochemistry-Paraffin: Factor VIII Antibody [NB100-91761] - IHC staining of Factor VIII in a formalin fixed and paraffin embedded tissue section of mouse bladder. The primary antibody was used at 1:100 dilution ...read more
Western Blot: Factor VIII Antibody [NB100-91761] - Western blot analysis of human placenta tissue lysate using Factor VIII antibody at 1:500 dilution.
Western Blot: Factor VIII Antibody [NB100-91761] - WB analysis of HepG2 whole cell lysate (lane 1), Mouse liver tissue lysate (lane 2), and Rat liver tissue lysate (lane 3) using Factor VIII antibody at 1:500 dilution ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Factor VIII Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of human Factor VII (between amino acids 2100-2250) [UniProt P00451]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
F8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry 1:50 - 1:100
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
ELISA was reported in scientific literature. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
267 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Factor VIII Lysate (NBL1-10413)
Publications
Read Publications using
NB100-91761 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Factor VIII Antibody

  • AHF
  • Antihemophilic factor
  • coagulation factor VIII, procoagulant component
  • coagulation factor VIIIc
  • DXS1253E
  • F8Ccoagulation factor VIII
  • factor VIII F8B
  • FVIII
  • HEMAF8B
  • Procoagulant component

Background

Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (prothrombin, and Factors X, IX, V, and VIII), which are involved in a blood coagulation cascade that leads to the formation of insoluble fibrin clots and the promotion of platelet aggregation. Coagulation factor VIII is a 2351 amino acid, non-covalent heterodimer, that circulates as an inactive procofactor. When catalyzed by thrombin, factor VIII is converted to active Factor VIIIa, which associates with factor IXa. This complex catalyzes the conversion of factor X to factor Xa. This results in the amplification phase of coagulation. [PMID: 16513527]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Factor VIII Antibody (NB100-91761)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: ELISA.


Filter By Application
ELISA
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
All Species

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Factor VIII Antibody (NB100-91761). (Showing 1 - 2 of 2 FAQs).

  1. I am interested in knowing whether your antibody (NB100-91761) can neutralize FVIII in rats.
    • Unfortunately, our product NB100-91761 has not yet been tested for blocking or neutralizing assays. We therefore do not have any data to support or deny this function.
  2. There is a western blot image in the product description testing human factor VIII in HepG2 whole cell lysate. I want to know whether the HepG2 used were untransfected WT cells or transfected by an FVIII-coding plasmid?
    • The Western blot data on our product page for NB100-91761 was generated from HepG2 whole cell lysate, mouse liver tissue lysate and rat liver tissue lysate. The HepG2 whole cell lysate was derived from endogenous cells; these had not been transfected to over-express the protein.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 Labeled NB100-91761AF488
Alexa Fluor 647 Labeled NB100-91761AF647
Biotin Labeled NB100-91761B
DyLight 350 Labeled NB100-91761UV
DyLight 405 Labeled NB100-91761V
DyLight 488 Labeled NB100-91761G
DyLight 550 Labeled NB100-91761R
DyLight 650 Labeled NB100-91761C
DyLight 680 Labeled NB100-91761FR
DyLight 755 Labeled NB100-91761IR
HRP Labeled NB100-91761H

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Blogs on Factor VIII.

Factor VIII - a key factor in the clotting process
Hemostasis, or blood clotting, follows tissue injury and involves the deployment of essential plasma procoagulants (such as prothrombin, and Factors X, IX, V, and VIII) that trigger the blood coagulation cascade. This cascade leads to the formation...  Read full blog post.

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Bioinformatics

Gene Symbol F8
Entrez
Uniprot