Factor VIII Antibody

Western Blot: Factor VIII Antibody [NB100-91761] - WB detection of Factor VIII protein in extracts from human umbilical vein endothelial cells (HUVECs).

Immunocytochemistry/Immunofluorescence: Factor VIII Antibody [NB100-91761] - Factor VIII antibody was tested 1:50 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.

Immunohistochemistry-Paraffin: Factor VIII Antibody [NB100-91761] - IHC staining of Factor VIII in a formalin fixed and paraffin embedded tissue section of mouse bladder. The primary antibody was used at 1:100 dilution and the detection was performed using DAB with hematoxylin counterstaining.

Western Blot: Factor VIII Antibody [NB100-91761] - Western blot analysis of human placenta tissue lysate using Factor VIII antibody at 1:500 dilution.

Western Blot: Factor VIII Antibody [NB100-91761] - WB analysis of HepG2 whole cell lysate (lane 1), Mouse liver tissue lysate (lane 2), and Rat liver tissue lysate (lane 3) using Factor VIII antibody at 1:500 dilution with ECL based detection.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Factor VIII Antibody Summary

• Immunogen: A synthetic peptide made to a C-terminal portion of human Factor VII (between amino acids 2100-2250) [UniProt P00451]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: F8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/ml
  • ELISA
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry 1:50 - 1:100
  • Immunohistochemistry-Paraffin 1:50-1:200
• Application Notes: ELISA was reported in scientific literature.
• Theoretical MW: 267 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Positive Control:

  Factor VIII Lysate (NBL1-10413)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Factor VIII Antibody

• AHF
• Antihemophilic factor
• coagulation factor VIII, procoagulant component
• coagulation factor VIIIc
• DXS1253E
• F8Ccoagulation factor VIII
• factor VIII F8B
• FVIII
• HEMAF8B
• Procoagulant component

Background

Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (prothrombin, and Factors X, IX, V, and VIII), which are involved in a blood coagulation cascade that leads to the formation of insoluble fibrin clots and the promotion of platelet aggregation. Coagulation factor VIII is a 2351 amino acid, non-covalent heterodimer, that circulates as an inactive procofactor. When catalyzed by thrombin, factor VIII is converted to active Factor VIIIa, which associates with factor IXa. This complex catalyzes the conversion of factor X to factor Xa. This results in the amplification phase of coagulation. [PMID: 16513527]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Factor VIII Antibody (NB100-91761)(2)

Publications using NB100-91761

• Zhu F, Liu Z, Wang X et al. Inter-chain disulfide bond improved protein trans-splicing increases plasma coagulation activity in C57BL/6 mice following portal vein FVIII gene delivery by dual vectors Sci China Life Sci 2013 Mar [PMID: 23526393] (ELISA, Human)
• Zhu F, Liu Z, Miao J, Qu H, Chi X. Enhanced plasma factor VIII activity in mice via cysteine mutation using dual vectors Sci China Life Sci 2012 Jun [PMID: 22744182] (ELISA, Human, Mouse)

FAQs for Factor VIII Antibody (NB100-91761). (Showing 1 - 2 of 2 FAQs).

1. I am interested in knowing whether your antibody (NB100-91761) can neutralize FVIII in rats.
   • Unfortunately, our product NB100-91761 has not yet been tested for blocking or neutralizing assays. We therefore do not have any data to support or deny this function.
2. There is a western blot image in the product description testing human factor VIII in HepG2 whole cell lysate. I want to know whether the HepG2 used were untransfected WT cells or transfected by an FVIII-coding plasmid?
   • The Western blot data on our product page for NB100-91761 was generated from HepG2 whole cell lysate, mouse liver tissue lysate and rat liver tissue lysate. The HepG2 whole cell lysate was derived from endogenous cells; these had not been transfected to over-express the protein.

Bioinformatics

• Gene Symbol: F8
• Entrez:
  • 2157(Human)
  • 14069(Mouse)
• Uniprot:
  • P00451(Human)