E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated]

Images

 
Human umbilical cord endothelial cells (HUVECs) were cultured for 6 hours in the presence of 25 ng/mL of rhTNF-alpha (210-TA, filled histogram) or rested (open histogram) and stained with Mouse anti-human ...read more
E-Selectin/CD62E was detected in immersion fixed HUVEC human umbilical vein endothelial cells activated with TNF‑ alpha (Catalog # 210-TA-010) using Mouse Anti-Human E‑Selectin/CD62E Monoclonal Antibody (Catalog # ...read more
E‑Selectin/CD62E was detected in immersion fixed paraffin-embedded sections of human brain (occipital cortex) using 10 µg/mL Mouse Anti-Human E‑Selectin/CD62E Monoclonal Antibody (Catalog # BBA16) overnight at 4 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IP, ELISA(Cap), ICC/IF
Clone
BBIG-E4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated] Summary

Additional Information
Clone BBIG-E4 was used by HLDA to establish CD designation
Immunogen
Activated HUVEC human umbilical vein endothelial cells
Specificity
Detects E‑Selectin/CD62E in ELISAs and Western blots. In immunofluorescence experiments, does not stain COS cells transfected with cDNA for human ICAM-1, PECAM‑1, L-Selectin, P-Selectin, or VCAM-1.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SELE
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-10 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation
  • Western Blot 1 ug/mL
Application Notes
ELISA Capture: Human E-Selectin/CD62E Antibody (Catalog # BBA16)
ELISA Detection: Human E-Selectin/CD62E Biotinylated Antibody (Catalog # BBA8)
Standard: Recombinant Human E-Selectin/CD62E (Catalog # ADP1)
Reviewed Applications
Read 1 Review rated 5
using
BBA16 in the following applications:

Publications
Read Publications using
BBA16 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated]

  • CD62 antigen-like family member E
  • CD62E antigen
  • CD62E
  • ELAM
  • ELAM1
  • ELAM-1
  • ELAM1E-selectin
  • endothelial adhesion molecule 1
  • Endothelial leukocyte adhesion molecule 1
  • ESEL
  • E-Selectin
  • LECAM2
  • leukocyte endothelial cell adhesion molecule 2
  • Leukocyte-endothelial cell adhesion molecule 2
  • SELE
  • selectin E

Background

E-Selectin, also known as Endothelial Leukocyte Adhesion Molecule 1 (ELAM-1), and Leukocyte-Endothelial Cell Adhesion Molecule 2 (LECAM-2), has been designated CD62E. It is a cell-surface glycoprotein expressed by activated endothelium and it mediates the adhesion of blood neutrophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for E-Selectin/CD62E Antibody (BBA16)(54)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 10 applications: BBA16, ELISA - Cell Based, ELISA Development, Flow Cytometry, Functional Assay, ICC, IHC, IHC-Fr, Neutralization, Western Blot.


Filter By Application
BBA16
(1)
ELISA - Cell Based
(1)
ELISA Development
(2)
Flow Cytometry
(1)
Functional Assay
(1)
ICC
(5)
IHC
(1)
IHC-Fr
(1)
Neutralization
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(16)
All Species
Showing Publications 1 - 10 of 54. Show All 54 Publications.
Publications using BBA16 Applications Species
T Pirker, EM Pferschy-W, E Bampali, V Bochkov, R Bauer Glycolipid-enriched fraction of Osmanthus fragrans inhibits LPS-induced expression of inflammatory genes, COX-2, E-selectin, and Interleukin-8 Journal of ethnopharmacology, 2023-03-02;0(0):116328. 2023-03-02 [PMID: 36870464] (BBA16, Human) BBA16 Human
S Mylvaganam, M Riedl, A Vega, RF Collins, K Jaqaman, S Grinstein, SA Freeman Stabilization of Endothelial Receptor Arrays by a Polarized Spectrin Cytoskeleton Facilitates Rolling and Adhesion of Leukocytes Cell Rep, 2020-06-23;31(12):107798. 2020-06-23 [PMID: 32579925] (ICC, Human) ICC Human
J Seigner, M Junker-Sam, A Plaza, G D'Urso, M Masullo, S Piacente, YM Holper-Sch, R de Martin A Symphytum officinale Root Extract Exerts Anti-inflammatory Properties by Affecting Two Distinct Steps of NF-?B Signaling Front Pharmacol, 2019-04-26;10(0):289. 2019-04-26 [PMID: 31105555] (ELISA - Cell Based, Human) ELISA - Cell Based Human
Q Su, Y Sun, Z Ye, H Yang, B Kong, L Li Pinocembrin protects endothelial cells from oxidized LDL-induced injury Cytokine, 2018-06-18;0(0):. 2018-06-18 [PMID: 29914794] (Western Blot, Human) Western Blot Human
R Kedmi, N Veiga, S Ramishetti, M Goldsmith, D Rosenblum, N Dammes, I Hazan-Hale, L Nahary, S Leviatan-B, M Harlev, M Behlke, I Benhar, J Lieberman, D Peer A modular platform for targeted RNAi therapeutics Nat Nanotechnol, 2018-01-29;0(0):. 2018-01-29 [PMID: 29379205]
C Donnelly, B Dykstra, N Mondal, J Huang, BJ Kaskow, R Griffin, R Sackstein, C Baecher-Al Optimizing human Treg immunotherapy by Treg subset selection and E-selectin ligand expression Sci Rep, 2018-01-11;8(1):420. 2018-01-11 [PMID: 29323143] (Western Blot, Human) Western Blot Human
BV Howley, LA Link, S Grelet, M El-Sabban, PH Howe A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer Oncogene, 2017-12-18;0(0):. 2017-12-18 [PMID: 29249802]
H Wan, B Tang, X Liao, Q Zeng, Z Zhang, L Liao Analysis of neuronal phosphoproteome reveals PINK1 regulation of BAD function and cell death Cell Death Differ., 2017-12-12;0(0):. 2017-12-12 [PMID: 29234155]
S Hasan, NN Kulkarni, A Asbjarnars, I Linhartova, R Osicka, P Sebo, GH Gudmundsso Bordetella pertussis adenylate cyclase toxin disrupts functional integrity of bronchial epithelial layers Infect. Immun., 2018-02-20;0(0):. 2018-02-20 [PMID: 29203545]
H Zhang, X Fang, D Huang, Q Luo, M Zheng, K Wang, L Cao, Z Yin Erythropoietin signaling increases neurogenesis and oligodendrogenesis of endogenous neural stem cells following spinal cord injury both in�vivo and in�vitro Mol Med Rep, 2017-10-25;0(0):. 2017-10-25 [PMID: 29115443]
Show All 54 Publications.

Review for E-Selectin/CD62E Antibody (BBA16) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using BBA16:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin E-Selectin/CD62E BBA16
Enlarge
5
reviewed by:
Michael Nowak
IHC-P Human 11/28/2021
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedColon adenocarcinoma tissue
SpeciesHuman
LotBBIG-E4

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Recent Reviews

5
5
1
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1
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Michael Nowak
11/28/2021
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol SELE