Reactivity | HuSpecies Glossary |
Applications | WB, Flow, IHC, IP, ELISA(Cap), ICC/IF |
Clone | BBIG-E4 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Concentration | LYOPH |
Additional Information | Clone BBIG-E4 was used by HLDA to establish CD designation |
Immunogen | Activated HUVEC human umbilical vein endothelial cells |
Specificity | Detects E‑Selectin/CD62E in ELISAs and Western blots. In immunofluorescence experiments, does not stain COS cells transfected with cDNA for human ICAM-1, PECAM‑1, L-Selectin, P-Selectin, or VCAM-1. |
Source | N/A |
Isotype | IgG1 |
Clonality | Monoclonal |
Host | Mouse |
Gene | SELE |
Purity Statement | Protein A or G purified from hybridoma culture supernatant |
Endotoxin Note | <0.10 EU per 1 μg of the antibody by the LAL method. |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | ELISA Capture: Human E-Selectin/CD62E Antibody (Catalog # BBA16) ELISA Detection: Human E-Selectin/CD62E Biotinylated Antibody (Catalog # BBA8) Standard: Recombinant Human E-Selectin/CD62E (Catalog # ADP1) |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Preservative | No Preservative |
Concentration | LYOPH |
Reconstitution Instructions | Sterile PBS to a final concentration of 0.5 mg/mL. |
Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Michael Nowak |
IHC-P | Human | 11/28/2021 |
Summary
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Secondary Antibodies |
Isotype Controls |
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Gene Symbol | SELE |