E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated]

Human umbilical cord endothelial cells (HUVECs) were cultured for 6 hours in the presence of 25 ng/mL of rhTNF-alpha (210-TA, filled histogram) or rested (open histogram) and stained with Mouse anti-human E-selectin/CD62E (BBA16) followed by Goat anti-Mouse APC-conjugated secondary antibody (F0101B). Gates were set based on isotype control (MAB002, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.

E-Selectin/CD62E was detected in immersion fixed HUVEC human umbilical vein endothelial cells activated with TNF‑ alpha (Catalog # 210-TA-010) using Mouse Anti-Human E‑Selectin/CD62E Monoclonal Antibody (Catalog # BBA16) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

E‑Selectin/CD62E was detected in immersion fixed paraffin-embedded sections of human brain (occipital cortex) using 10 µg/mL Mouse Anti-Human E‑Selectin/CD62E Monoclonal Antibody (Catalog # BBA16) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, Flow, IHC, IP, ELISA(Cap), ICC/IF
• Clone: BBIG-E4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated] Summary

• Additional Information: Clone BBIG-E4 was used by HLDA to establish CD designation
• Immunogen: Activated HUVEC human umbilical vein endothelial cells
• Specificity: Detects E‑Selectin/CD62E in ELISAs and Western blots. In immunofluorescence experiments, does not stain COS cells transfected with cDNA for human ICAM-1, PECAM‑1, L-Selectin, P-Selectin, or VCAM-1.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SELE
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-10 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation
  • Western Blot 1 ug/mL
• Application Notes: ELISA Capture: Human E-Selectin/CD62E Antibody (Catalog # BBA16)
  ELISA Detection: Human E-Selectin/CD62E Biotinylated Antibody (Catalog # BBA8)
  Standard: Recombinant Human E-Selectin/CD62E (Catalog # ADP1)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated]

• CD62 antigen-like family member E
• CD62E antigen
• CD62E
• ELAM
• ELAM1
• ELAM-1
• ELAM1E-selectin
• endothelial adhesion molecule 1
• Endothelial leukocyte adhesion molecule 1
• ESEL
• E-Selectin
• LECAM2
• leukocyte endothelial cell adhesion molecule 2
• Leukocyte-endothelial cell adhesion molecule 2
• SELE
• selectin E

Background

E-Selectin, also known as Endothelial Leukocyte Adhesion Molecule 1 (ELAM-1), and Leukocyte-Endothelial Cell Adhesion Molecule 2 (LECAM-2), has been designated CD62E. It is a cell-surface glycoprotein expressed by activated endothelium and it mediates the adhesion of blood neutrophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for E-Selectin/CD62E Antibody (BBA16)(54)

Publications using BBA16

• T Pirker, EM Pferschy-W, E Bampali, V Bochkov, R Bauer Glycolipid-enriched fraction of Osmanthus fragrans inhibits LPS-induced expression of inflammatory genes, COX-2, E-selectin, and Interleukin-8 Journal of ethnopharmacology, 2023-03-02;0(0):116328. 2023-03-02 [PMID: 36870464] (BBA16, Human)
• S Mylvaganam, M Riedl, A Vega, RF Collins, K Jaqaman, S Grinstein, SA Freeman Stabilization of Endothelial Receptor Arrays by a Polarized Spectrin Cytoskeleton Facilitates Rolling and Adhesion of Leukocytes Cell Rep, 2020-06-23;31(12):107798. 2020-06-23 [PMID: 32579925] (ICC, Human)
• J Seigner, M Junker-Sam, A Plaza, G D'Urso, M Masullo, S Piacente, YM Holper-Sch, R de Martin A Symphytum officinale Root Extract Exerts Anti-inflammatory Properties by Affecting Two Distinct Steps of NF-?B Signaling Front Pharmacol, 2019-04-26;10(0):289. 2019-04-26 [PMID: 31105555] (ELISA - Cell Based, Human)
• Q Su, Y Sun, Z Ye, H Yang, B Kong, L Li Pinocembrin protects endothelial cells from oxidized LDL-induced injury Cytokine, 2018-06-18;0(0):. 2018-06-18 [PMID: 29914794] (Western Blot, Human)
• R Kedmi, N Veiga, S Ramishetti, M Goldsmith, D Rosenblum, N Dammes, I Hazan-Hale, L Nahary, S Leviatan-B, M Harlev, M Behlke, I Benhar, J Lieberman, D Peer A modular platform for targeted RNAi therapeutics Nat Nanotechnol, 2018-01-29;0(0):. 2018-01-29 [PMID: 29379205]
• C Donnelly, B Dykstra, N Mondal, J Huang, BJ Kaskow, R Griffin, R Sackstein, C Baecher-Al Optimizing human Treg immunotherapy by Treg subset selection and E-selectin ligand expression Sci Rep, 2018-01-11;8(1):420. 2018-01-11 [PMID: 29323143] (Western Blot, Human)
• BV Howley, LA Link, S Grelet, M El-Sabban, PH Howe A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer Oncogene, 2017-12-18;0(0):. 2017-12-18 [PMID: 29249802]
• H Wan, B Tang, X Liao, Q Zeng, Z Zhang, L Liao Analysis of neuronal phosphoproteome reveals PINK1 regulation of BAD function and cell death Cell Death Differ., 2017-12-12;0(0):. 2017-12-12 [PMID: 29234155]
• S Hasan, NN Kulkarni, A Asbjarnars, I Linhartova, R Osicka, P Sebo, GH Gudmundsso Bordetella pertussis adenylate cyclase toxin disrupts functional integrity of bronchial epithelial layers Infect. Immun., 2018-02-20;0(0):. 2018-02-20 [PMID: 29203545]
• H Zhang, X Fang, D Huang, Q Luo, M Zheng, K Wang, L Cao, Z Yin Erythropoietin signaling increases neurogenesis and oligodendrogenesis of endogenous neural stem cells following spinal cord injury both in�vivo and in�vitro Mol Med Rep, 2017-10-25;0(0):. 2017-10-25 [PMID: 29115443]
• L Fülle, N Steiner, M Funke, F Gondorf, F Pfeiffer, J Siegl, FV Opitz, SK Ha beta el, AB Erazo, O Schanz, HJ Stunden, M Blank, C Gröber, K Händler, M Beyer, H Weighardt, E Latz, JL Schultze, G Mayer, I Förster RNA Aptamers Recognizing Murine CCL17 Inhibit T Cell Chemotaxis and Reduce Contact Hypersensitivity In�Vivo Mol. Ther., 2017-10-13;0(0):. 2017-10-13 [PMID: 29103909]
• K Yan, Y Shen Aliskiren has chondroprotective efficacy in a rat model of osteoarthritis through suppression of the local renin-angiotensin system Mol Med Rep, 2017-07-28;0(0):. 2017-07-28 [PMID: 28765966]
• A Tang, C Li, Z Chen, T Li Anti-CD20 monoclonal antibody combined with adenovirus vector-mediated IL-10 regulates spleen CD4+/CD8+ T�cells and T-bet/GATA-3 expression in NOD mice Mol Med Rep, 2017-07-28;0(0):. 2017-07-28 [PMID: 28765956]
• S Ganesan, TL Thanh, N Kadri, BJ Chambers, S Meinke, P Brodin, E Vivier, DM Wetzel, AJ Koleske, P Höglund The Abl-1 kinase is dispensable for NK cell inhibitory signaling and is not involved in murine NK cell education Scand. J. Immunol., 2017-09-01;0(0):. 2017-09-01 [PMID: 28605050]
• X Lin, J Lv, J Lv, C Di, Y Zhang, T Zhou, J Liu, Z Xia Heme oxygenase-1 directly binds STAT3 to control the generation of pathogenic Th17 cells during neutrophilic airway inflammation Allergy, 2017-07-03;0(0):. 2017-07-03 [PMID: 28580587]
• JC Zhang, W Yao, C Dong, C Yang, Q Ren, M Ma, K Hashimoto Blockade of interleukin-6 receptor in the periphery promotes rapid and sustained antidepressant actions: a possible role of gut-microbiota-brain axis Transl Psychiatry, 2017-05-30;7(5):e1138. 2017-05-30 [PMID: 28556833]
• W Stallaert, ET van der We, AM Schonegge, B Plouffe, M Hogue, V Lukashova, A Inoue, S Ishida, J Aoki, C Le Gouill, M Bouvier Purinergic receptor transactivation by the ?2-adrenergic receptor increases intracellular Ca(2+) in non-excitable cells Mol. Pharmacol, 2017-03-09;0(0):. 2017-03-09 [PMID: 28280061]
• F Marttila-I, K Elima, K Auvinen, TZ Veres, P Rantakari, C Weston, M Miyasaka, D Adams, S Jalkanen, M Salmi Amine oxidase activity regulates the development of pulmonary fibrosis FASEB J, 2017-03-01;0(0):. 2017-03-01 [PMID: 28251930]
• J Ratanavara, K Soontornvi, S Shuangshot, S Shuangshot, S Damrongsak Localized delivery of curcumin from injectable gelatin/Thai silk fibroin microspheres for anti-inflammatory treatment of osteoarthritis in a rat model Inflammopharmacology, 2017-03-01;0(0):. 2017-03-01 [PMID: 28251487]
• YF Ding, ZH Wu, YJ Wei, L Shu, YR Peng Hepatic inflammation-fibrosis-cancer axis in the rat hepatocellular carcinoma induced by diethylnitrosamine J. Cancer Res. Clin. Oncol, 2017-02-25;0(0):. 2017-02-25 [PMID: 28238064]
• JP Vernot, X Bonilla, V Rodriguez-, NP Vanegas Phenotypic and Functional Alterations of Hematopoietic Stem and Progenitor Cells in an In Vitro Leukemia-Induced Microenvironment Int J Mol Sci, 2017-02-14;18(2):. 2017-02-14 [PMID: 28216566]
• K Yamaguchi, H Iwamoto, Y Horimasu, S Ohshimo, K Fujitaka, H Hamada, W Mazur, N Kohno, N Hattori AGER gene polymorphisms and soluble receptor for advanced glycation end product in patients with idiopathic pulmonary fibrosis Respirology, 2017-02-14;0(0):. 2017-02-14 [PMID: 28198072]
• Bernhard Wernly Soluble ST2 Predicts 1-Year Outcome in Patients Undergoing Transcatheter Aortic Valve Implantation (TAVI) Eur. J. Clin. Invest, 2017-02-01;0(0):. 2017-02-01 [PMID: 28036122]
• Xuejun Cheng Rat astrocytes are more supportive for mouse OPC self-renewal than mouse astrocytes in culture Dev Neurobiol, 2017-03-23;0(0):. 2017-03-23 [PMID: 28033654]
• Gong-Jhe Wu Protection of Dexmedetomidine Against Ischemia/Reperfusion-Induced Apoptotic Insults to Neuronal Cells Occurs via an Intrinsic Mitochondria-Dependent Pathway J. Cell. Biochem, 2017-05-16;0(0):. 2017-05-16 [PMID: 27987330]
• Lin Li IL-17RB(+) granulocytes are associated with airflow obstruction in asthma Ann. Allergy Asthma Immunol, 2016-12-01;117(6):674-679. 2016-12-01 [PMID: 27979026]
• Tra M T Bui Presence of vascular endothelial growth factor during the first half of IVM improves the meiotic and developmental competence of porcine oocytes from small follicles Reprod. Fertil. Dev, 2017-09-01;0(0):. 2017-09-01 [PMID: 27938625]
• Minu Karthika Ganesan 3D co-culture model to analyze the crosstalk between endothelial and smooth muscle cells Tissue Eng Part C Methods, 2017-01-01;0(0):. 2017-01-01 [PMID: 27923320] (ICC, Human)
• Dimitrios Agas INF-? encoding plasmid administration triggers bone loss and disrupts bone marrow microenvironment J. Endocrinol, 2016-12-01;0(0):. 2016-12-01 [PMID: 27908965]
• Trevor R F Smith Characterization of guinea pig T cell responses elicited after EP-assisted delivery of DNA vaccines to the skin Vaccine, 2016-11-25;35(1):61-70. 2016-11-25 [PMID: 27894716]
• Zhonghao Liu Inhibition of Adenylyl Cyclase in the Spinal Cord Alleviates Painful Diabetic Neuropathy in Zucker Diabetic Fatty Rats Can J Diabetes, 2016-11-23;0(0):. 2016-11-23 [PMID: 27889175]
• Jun Tao BMP4/Id2 signaling pathway is a novel therapeutic target for late outgrowth endothelial progenitor cell-mediated endothelial injury repair Int. J. Cardiol., 2016-11-09;228(0):796-804. 2016-11-09 [PMID: 27888757]
• Georgi Guruli Role of Epigenetic Modification and Immunomodulation in a Murine Prostate Cancer Model Prostate, 2016-11-08;0(0):. 2016-11-08 [PMID: 27862100]
• Simon X Liang Proliferation, differentiation and migration of SCA1(-)/CD31(-) cardiac side population cells in vitro and in vivo Int. J. Cardiol., 2016-11-09;0(0):. 2016-11-09 [PMID: 27847151]
• Xiaoye Yan Neutrophil gelatinase-associated lipocalin predicts myocardial dysfunction and mortality in severe sepsis and septic shock Int. J. Cardiol., 2016-11-03;0(0):. 2016-11-03 [PMID: 27836304]
• Bruno Rodrigues Impact of exercise training associated to pyridostigmine treatment on autonomic function and inflammatory profile after myocardial infarction in rats Int. J. Cardiol., 2016-10-26;0(0):. 2016-10-26 [PMID: 27823896]
• Cell surface-anchored syndecan-1 ameliorates intestinal inflammation and neutrophil transmigration in ulcerative colitis J Cell Mol Med, 2016-08-25;0(0):. 2016-08-25 [PMID: 27558380]
• C13orf31 (FAMIN) is a central regulator of immunometabolic function Nat Immunol, 2016-08-01;0(0):. 2016-08-01 [PMID: 27478939]
• Endothelin-1 Overexpression Exaggerates Diabetes-Induced Endothelial Dysfunction by Altering Oxidative Stress Am J Hypertens, 2016-11-01;0(0):. 2016-11-01 [PMID: 27465439]
• Plasma membrane TLR activation increases bacterial uptake but abrogates endosomal Lactobacillus acidophilus induction of Interferon-B Immunology, 2016-08-24;0(0):. 2016-08-24 [PMID: 27441725]
• Christoph Wilhelm, Oliver J Harrison, Vanessa Schmitt, Martin Pelletier, Sean P Spencer, Joseph F Urban, Michelle Ploch, Thirumalai R Ramalinga, Richard M Siegel, Yasmine Belkaid Critical role of fatty acid metabolism in ILC2-mediated barrier protection during malnutrition and helminth infection. The Journal of Experimental Medicine, 2016-07-18;0(0):1540-9538. 2016-07-18 [PMID: 27432938]
• ME Legrier, I BiŠche, J Gaston, A Beurdeley, V Yvonnet, O D‚as, A Thuleau, S Chƒteau-Jo, JL Servely, S Vacher, M Lassalle, S Depil, GC Tucker, JJ Fontaine, MF Poupon, S Roman-Roma, JG Judde, D Decaudin, S Cairo, E Marangoni Activation of IFN/STAT1 signalling predicts response to chemotherapy in oestrogen receptor-negative breast cancer Br. J. Cancer, 2015-12-22;114(2):177-87. 2015-12-22 [PMID: 26695443]
• Wang F, Hou H, Wu S, Tang Q, Liu W, Huang M, Yin B, Huang J, Mao L, Lu Y, Sun Z TIGIT expression levels on human NK cells correlate with functional heterogeneity among healthy individuals. Eur J Immunol, 2015-09-07;45(10):2886-97. 2015-09-07 [PMID: 26171588]
• Kang S, Hasan N, Mann A, Zheng W, Zhao L, Morris L, Zhu W, Zhao Y, Suh K, Dooley W, Volk D, Gorenstein D, Cristofanilli M, Rui H, Tanaka T Blocking the adhesion cascade at the premetastatic niche for prevention of breast cancer metastasis. Mol Ther, 2015-03-27;23(6):1044-54. 2015-03-27 [PMID: 25815697]
• Pezeshkian , Bahareh, Donnelly , Christop, Tamburo , Kelley, Geddes , Timothy, Madlambayan , Gerard J Leukemia Mediated Endothelial Cell Activation Modulates Leukemia Cell Susceptibility to Chemotherapy through a Positive Feedback Loop Mechanism. PLoS ONE, 2013-04-01;8(4):e60823. 2013-04-01 [PMID: 23560111] (Functional Assay, Human)
• Schellerer VS, Croner RS, Weinlander K, Hohenberger W, Sturzl M, Naschberger E Endothelial cells of human colorectal cancer and healthy colon reveal phenotypic differences in culture. Lab. Invest., 2007-09-17;87(11):1159-70. 2007-09-17 [PMID: 17876298] (ICC, Human)
• Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium. J. Exp. Med., 2006-11-20;203(12):2763-77. 2006-11-20 [PMID: 17116732] (ICC, Human)
• Prakobphol A, Genbacev O, Gormley M, Kapidzic M, Fisher SJ A role for the L-selectin adhesion system in mediating cytotrophoblast emigration from the placenta. Dev. Biol., 2006-06-15;298(1):107-17. 2006-06-15 [PMID: 16930583] (IHC-Fr, Human)
• Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damas JK Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes. Blood, 2006-08-01;108(3):928-35. 2006-08-01 [PMID: 16861346] (ELISA Development, Human)
• Amin MA, Haas CS, Zhu K, Mansfield PJ, Kim MJ, Lackowski NP, Koch AE Migration inhibitory factor up-regulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 via Src, PI3 kinase, and NFkappaB. Blood, 2005-11-29;107(6):2252-61. 2005-11-29 [PMID: 16317091] (Neutralization, Human)
• Armitage JD, Lindsey NJ, Homer-Vanniasinkam S The role of endothelial cell reactive antibodies in peripheral arterial disease. Eur J Vasc Endovasc Surg, 2005-10-14;31(2):170-5. 2005-10-14 [PMID: 16226905] (ELISA Development, Human)
• Frattini J, Kudo FA Differential effects of orbital and laminar shear stress on endothelial cells. J. Vasc. Surg., 2005-05-01;41(5):869-80. 2005-05-01 [PMID: 15886673] (ICC, Human)
• Helantera I, Loginov R, Koskinen P, Tornroth T, Gronhagen-Riska C, Lautenschlager I Persistent cytomegalovirus infection is associated with increased expression of TGF-beta1, PDGF-AA and ICAM-1 and arterial intimal thickening in kidney allografts. Nephrol. Dial. Transplant., 2005-02-16;20(4):790-6. 2005-02-16 [PMID: 15716293] (IHC, Human)
• Remy-Zolghadri M, Laganiere J, Oligny JF, Germain L, Auger FA Endothelium properties of a tissue-engineered blood vessel for small-diameter vascular reconstruction. J. Vasc. Surg., 2004-03-01;39(3):613-20. 2004-03-01 [PMID: 14981456] (Flow Cytometry, Human)

Review for E-Selectin/CD62E Antibody (BBA16) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Michael Nowak IHC-P Human 11/28/2021

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Colon adenocarcinoma tissue
• Species: Human
• Lot: BBIG-E4

Bioinformatics

• Gene Symbol: SELE