E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated]

Images

 
E-Selectin/CD62E was detected in immersion fixed HUVEC human umbilical vein endothelial cells activated with TNF‑ alpha (Catalog # 210-TA-010) using Mouse Anti-Human E‑Selectin/CD62E Monoclonal Antibody (Catalog # ...read more
E‑Selectin/CD62E was detected in immersion fixed paraffin-embedded sections of human brain (occipital cortex) using 10 µg/mL Mouse Anti-Human E‑Selectin/CD62E Monoclonal Antibody (Catalog # BBA16) overnight at 4 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP, ELISA(Cap), ICC/IF
Clone
BBIG-E4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated] Summary

Additional Information
Clone BBIG-E4 was used by HLDA to establish CD designation
Immunogen
Activated HUVEC human umbilical vein endothelial cells
Specificity
Detects E‑Selectin/CD62E in ELISAs and Western blots. In immunofluorescence experiments, does not stain COS cells transfected with cDNA for human ICAM-1, PECAM‑1, L-Selectin, P-Selectin, or VCAM-1.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SELE
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation
  • ELISA Capture (Matched Antibody Pair) 2-10 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • Immunocytochemistry 8-25 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Human E-Selectin/CD62E Antibody (Catalog # BBA16)
ELISA Detection: Human E-Selectin/CD62E Biotinylated Antibody (Catalog # BBA8)
Standard: Recombinant Human E-Selectin/CD62E (Catalog # ADP1)
Publications
Read Publications using
BBA16 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated]

  • CD62 antigen-like family member E
  • CD62E antigen
  • CD62E
  • ELAM
  • ELAM1
  • ELAM-1
  • ELAM1E-selectin
  • endothelial adhesion molecule 1
  • Endothelial leukocyte adhesion molecule 1
  • ESEL
  • E-Selectin
  • LECAM2
  • leukocyte endothelial cell adhesion molecule 2
  • Leukocyte-endothelial cell adhesion molecule 2
  • SELE
  • selectin E

Background

E-Selectin, also known as Endothelial Leukocyte Adhesion Molecule 1 (ELAM-1), and Leukocyte-Endothelial Cell Adhesion Molecule 2 (LECAM-2), has been designated CD62E. It is a cell-surface glycoprotein expressed by activated endothelium and it mediates the adhesion of blood neutrophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for E-Selectin/CD62E Antibody (BBA16)(38)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 8 applications: ELISA Development, Flow Cytometry, Functional Assay, ICC, IHC, IHC Frozen, Neutralization, Western Blot.


Filter By Application
ELISA Development
(2)
Flow Cytometry
(1)
Functional Assay
(1)
ICC
(4)
IHC
(1)
IHC Frozen
(1)
Neutralization
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(13)
All Species
Showing Publications 1 - 10 of 38. Show All 38 Publications.
Publications using BBA16 Applications Species
Q Su, Y Sun, Z Ye, H Yang, B Kong, L Li Pinocembrin protects endothelial cells from oxidized LDL-induced injury Cytokine, 2018;0(0):. 2018 [PMID: 29914794] (Western Blot, Human) Western Blot Human
R Kedmi, N Veiga, S Ramishetti, M Goldsmith, D Rosenblum, N Dammes, I Hazan-Hale, L Nahary, S Leviatan-B, M Harlev, M Behlke, I Benhar, J Lieberman, D Peer A modular platform for targeted RNAi therapeutics Nat Nanotechnol, 2018;0(0):. 2018 [PMID: 29379205]
C Donnelly, B Dykstra, N Mondal, J Huang, BJ Kaskow, R Griffin, R Sackstein, C Baecher-Al Optimizing human Treg immunotherapy by Treg subset selection and E-selectin ligand expression Sci Rep, 2018;8(1):420. 2018 [PMID: 29323143] (Western Blot, Human) Western Blot Human
BV Howley, LA Link, S Grelet, M El-Sabban, PH Howe A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer Oncogene, 2017;0(0):. 2017 [PMID: 29249802]
H Wan, B Tang, X Liao, Q Zeng, Z Zhang, L Liao Analysis of neuronal phosphoproteome reveals PINK1 regulation of BAD function and cell death Cell Death Differ., 2017;0(0):. 2017 [PMID: 29234155]
S Hasan, NN Kulkarni, A Asbjarnars, I Linhartova, R Osicka, P Sebo, GH Gudmundsso Bordetella pertussis adenylate cyclase toxin disrupts functional integrity of bronchial epithelial layers Infect. Immun., 2017;0(0):. 2017 [PMID: 29203545]
H Zhang, X Fang, D Huang, Q Luo, M Zheng, K Wang, L Cao, Z Yin Erythropoietin signaling increases neurogenesis and oligodendrogenesis of endogenous neural stem cells following spinal cord injury both in�vivo and in�vitro Mol Med Rep, 2017;0(0):. 2017 [PMID: 29115443]
K Yan, Y Shen Aliskiren has chondroprotective efficacy in a rat model of osteoarthritis through suppression of the local renin-angiotensin system Mol Med Rep, 2017;0(0):. 2017 [PMID: 28765966]
A Tang, C Li, Z Chen, T Li Anti-CD20 monoclonal antibody combined with adenovirus vector-mediated IL-10 regulates spleen CD4+/CD8+ T�cells and T-bet/GATA-3 expression in NOD mice Mol Med Rep, 2017;0(0):. 2017 [PMID: 28765956]
S Ganesan, TL Thanh, N Kadri, BJ Chambers, S Meinke, P Brodin, E Vivier, DM Wetzel, AJ Koleske, P Höglund The Abl-1 kinase is dispensable for NK cell inhibitory signaling and is not involved in murine NK cell education Scand. J. Immunol., 2017;0(0):. 2017 [PMID: 28605050]
Show All 38 Publications.

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Bioinformatics

Gene Symbol SELE