Anti-CD31/PECAM-1 Antibodies: Novus Biologicals

AF3628

CD31/PECAM-1 Antibody [Unconjugated]

(6 Reviews)

100 μg

AF3628

HAF017). A specific band was detected for CD31/PECAM-1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF017). A specific band was detected for CD31/PECAM-1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for CD31/PECAM-1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Simple Western shows lysates of bEnd.3 mouse endothelioma cell line, loaded at 0.5 mg/ml. A specific band was detected for CD31/PECAM‑1 at approximately 165 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108)." class="big_lightbox">

Mouse splenocytes were stained with Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or control antibody (<a class=

AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108)." class="big_thumb" />

AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108)." class="big_lightbox">

Rat splenocytes were stained with Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or isotype control antibody (<a class=

AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108)." class="big_thumb" />

NL003) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

CD31/PECAM-1 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; <a class=

NL003) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to developing endothelium. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox">

CD31/PECAM-1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to developing endothelium. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing guts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

CD31/PECAM-1 was detected in immersion fixed frozen sections of mouse embryo (14 d.p.c.) using Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (<a class=

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing guts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in vasculature." class="big_lightbox">

CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of rat heart using Goat Anti-Human/Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (<a class=

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in vasculature." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in chorionic villi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of Human Placenta using Goat Anti-Human/Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in chorionic villi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

HUVEC cells were stained with Goat Anti-Human/Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or isotype control antibody (Catalog # <a class=

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

AF3628) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD31/PECAM‑1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 0.5 µg/ml of Goat Anti-Human/Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (<a class=

AF3628) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD31/PECAM‑1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

Representative image of immunostaining corresponding to the glucose transporter protein SLC2A1 (GLUT1) in 30 week-old Ins2AKITA and WT cerebral cortex. Mural cells (ANPEP, green), glucose transporter 1 (GLUT1, red), endothelium (PECAM1, cyan). n = 2, scale bar 50 µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30498224), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>AcSDKP suppresses TGF beta /smad signaling and EndMT through the FGFR1/FRS2 pathway. (a) HMVECs were treated with N-FGFR1 for 48 h, and the FGFR1, TGF beta R1 and TGF beta R2 protein levels were analyzed by western blot. (b) HMVECs were treated with TGF beta 2 in the presence or absence of N-FGFR1 for 15 min with or without AcSDKP preincubation. The p-smad3 and TGF beta R1 protein levels were analyzed by western blot. Densitometric analysis of the p-smad3/smad3 and TGF beta R1/ beta -actin levels (n=3) in each group was performed. (c) HMVECs were incubated with either N-FGFR1 in the presence or absence of TGF beta 2 for 48 h with or without preincubation with AcSDKP for 2 h or with N-FGFR1 in the presence or absence of TGF beta 2 for 48 h with or without 24 h of incubation with FGF2 (50 ng/ml). The CD31, SM22 alpha , FSP1 and alpha -SMA protein levels were analyzed by western blot. (d) HMVECs were transfected with FRS2 siRNA (100 nM) for 48 h with or without AcSDKP preincubation. The VE-cadherin, FSP1, vimentin, SM22 alpha and p-smad3 levels were analyzed by western blot. (e) HMVECs were treated with N-FGFR1 for 48 h or 15 min in the presence or absence of N-TGF beta (1, 2, 3) (1.0 μg/ml). The CD31, VE-cadherin, SM22 alpha , FSP1, TGF beta R1, TGF beta R2 and p-smad3 levels were analyzed by western blot Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28771231), licensed under a CC-BY license. Not internally tested by R&D Systems.

AcSDKP inhibits TGF beta /smad signaling and EndMT and restores the FGFR1 and P-MAP4K4 levels in diabetic hearts. (a) Immunofluorescence microscopy analysis of CD31/FGFR1 and CD31/P-MAP4K4 in the heart tissues from each group of mice. The scale bar is 60 μm in each panel. The CD31 and FGFR1 double-labeled cells and the CD31 and P-MAP4K4 double-labeled cells in each visual field were assessed by fluorescence microscopy and quantified. For each section, images from six different fields of view at × 400 magnification were evaluated. (b and c) Immunofluorescence microscopy analysis of CD31/ alpha -SMA, VE-cadherin /SM22 alpha and CD31/p-smad3 expression levels in the heart tissues from each group of mice. The scale bar is 60 μm in each panel. The CD31 and alpha -SMA double-labeled cells, the VE-cadherin and SM22 alpha double-labeled cells and the CD31 and p-smad3 double-labeled cells in each visual field were analyzed by fluorescence microscopy and quantified. For each section, images from six different fields of view at × 400 magnification were evaluated. Four mice from each group were analyzed. (d) Western blot analysis of the FGFR1, P-MAP4K4, TGF beta 1, TGF beta 2 and TGF beta 3 levels in cardiac tissues. A representative blot from four independent experiments was shown. The densitometric analysis of western blot data was presented (n=4). The diabetic mice are abbreviated as DM in the figure Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28771231), licensed under a CC-BY license. Not internally tested by R&D Systems.

Diminished permeability of liver sinusoids in Plvap-deficient mice.Neither by immunohistochemistry with antibodies against CD31 (A) nor by light microscopy of 1 µm semi-thin sections (B, Richardson's stain) obvious differences are detected with regards to the overall orientation and the density of liver sinusoids between 3-week-old Plvap-/- mice and wild-type littermates. Sinusoids of Plvap-deficient mice show a higher number of macrophages in their lumen (white arrows) and focal areas with accumulations of mononuclear cells in Disse's space (black arrows). Lower panels in B show higher magnifications. C, After perfusion of a wild-type animal with FITC-dextran, a strong FITC-signal (green) throughout the liver is detected. Immunolabeling with CD31 (red) suggests that FITC-dextran molecules have accumulated in the space of Disse. In contrast, in the Plvap-deficient littermate, the signal for FITC-dextran is much weaker and barely detectable. Nuclear DNA is labeled with DAPI (blue). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0115005), licensed under a CC-BY license. Not internally tested by R&D Systems.

Blood-brain barrier permeability measurements in male Ins2AKITA and WT littermate controls. (a) Representative stereomicroscope fluorescence images of brains showing 1 kDa Alexa Fluor 555 cadaverine permeability in Ins2AKITA and WT after 2 h of dye circulation (n = 2). (b) Representative confocal images of coronal sections of 1 kDa Alexa Fluor 555 cadaverine injected mouse brains. ANPEP positive mural cells, green; PECAM1 positive vasculature, white. No Alexa Fluor 555 cadaverine leakage into brain parenchyma was observed either in Ins2AKITA or WT mice (n = 2, scale bar 30 μm). (c) Quantification of 1 kDa Alexa Fluor 555 cadaverine permeability in 26.5–32 week-old Ins2AKITA and WT mice after 2 h circulation (n = > 8, 3 independent experiments). y-axis shows the fold change in relative fluorescence units (RFU) per gram of brain tissue in relation to WT. (d) Quantification of 1 kDa Alexa Fluor 488 cadaverine permeability in 38 week-old Ins2AKITA and WT mice after 2 h circulation (n = 3). y-axis shows the fold change in relative fluorescence units (RFU) per gram of brain tissue in relation to WT. (e) Evans Blue dye permeability in 30 week-old Ins2AKITA and WT littermate control mice after overnight circulation (n = > 2). y-axis shows optical density (OD) at 620 nm per gram of tissue. PdgfbRet/Ret served as positive control for tracer leakage into the brain parenchyma. n.s., not significant, student’s t test. Data is presented as mean ± SEM. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30498224), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>CL and low temperature lead to WAT browning, angiogenesis, and an increased number of myofibroblast-like cells. a, b Histological analysis of adipocyte morphology (H&E), adipocytes (PERI), mitochondria (COX4), uncoupling protein 1 (UCP1), blood vessels (CD31), and myofibroblast-like cells ( alpha SMA) in a 5-day CL-316243-treated scWAT and visWAT compared to vehicle-treated control. b Two-week 4 °C-treated scWAT and visWAT compared to 30 °C control. Double-headed arrows mark adipocyte diameter. Arrows point to respective positive signals. c–l Quantifications of adipocyte size and positive signals per field of COX4, UCP1, CD31, and alpha SMA of CL-316243- and vehicle-, and 30 °C- and 4 °C- treated scWATs and visWATs (>30 adipocytes per field; n = 10 random fields; n = 5 mice per group). PERI, perilipin; COX4, mitochondrial complex 4; UCP1, uncoupling protein 1. Scale bars, 100 μm. NS, not significant. *P < 0.05, **P < 0.01, and ***P < 0.001 by Student’s t-test. Data presented as mean ± s.e.m. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-02158-z), licensed under a CC-BY license. Not internally tested by R&D Systems.

AB-108-C, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

Whole blood granulocytes were stained with Goat Anti-Human/Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or isotype control antibody (Catalog # <a class=

AB-108-C, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

Neutrophil depletion reduces BBB breakdown and increases neovascularization after stroke. f, g Representative confocal images (f) and quantitative analysis of IgG extravascular deposits (g) in the peri-infarct cortex at 14 days in sham-operated mice and mice treated with control antibody or anti-Ly6G antibody (n = 6). One-way ANOVA test was applied with *P < 0.0001 (Sham vs. Isotype), *P = 0.0041 (Isotype vs. Anti-Ly6G). Bar = 15 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32427863), licensed under a CC-BY license. Not internally tested by R&D Systems.

Increased vascular remodeling by delayed inhibition of NET formation.a–d Representative confocal images (a, c) and quantitative analysis of IgG extravascular deposits (b, d) in the peri-infarct cortex at 14 days. Mice were subjected to stroke and treated with either anti-Ly6G antibody, control antibody, DNase 1, or vehicle starting at 7 days (n = 6), unpaired two-tailed Student’s t-test was applied with *P = 0.0392 (b), *P = 0.0384 (d). Bar = 10 μm. e–l Representative confocal images (e, g) of CD31-positive microvessels and in-vivo multiphoton microscopy images of perfused cortical capillaries with intravenously injected FITC-dextran (i, k) in the peri-infarct cortex at 14 days in mice treated with either anti-Ly6G antibody, control antibody, DNase 1, or vehicle. Bar = 40 μm (e, g) and 100 µm (i, k). Quantification of microvascular density (f, h) and perfused capillary length (j, l) for each group (n = 6), unpaired two-tailed Student’s t-test was applied with *P = 0.00378 (f), *P = 0.0364 (h), *P = 0.0026 (j), *P = 0.0006 (l). Data are presented as mean ± SD. Source data underlying graph b, d, f, h, j, and l are provided as a Source Data file. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32427863), licensed under a CC-BY license. Not internally tested by R&D Systems.

Neutrophil depletion reduces BBB breakdown and increases neovascularization after stroke. h, j Representative confocal images (h) of CD31-positive microvessels and in-vivo multiphoton microscopy images of perfused cortical capillaries with intravenously injected FITC-dextran (MW = 2000,000 Da) (j) in the peri-infarct cortex at 14 days in mice treated with control antibody or anti-Ly6G antibody, compared with sham-operated mice. Bar = 50 μm (e) and 100 µm (g) Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32427863), licensed under a CC-BY license. Not internally tested by R&D Systems.

Neutrophils accumulate in the brain during all stages of ischemic stroke. f Representative confocal images of Ly6G-labeled neutrophils (green) and CD31-positive microvessels (white) in the peri-infarct cortex of mice at 3 days. Nuclei were visualized with Hoechst. Neutrophils were observed within brain vessels and migrated into the parenchyma. Bar = 40 μm. Independent experiments are repeated at least three times. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32427863), licensed under a CC-BY license. Not internally tested by R&D Systems.

Microvascular and tumor cell biology at the tumor rim in MDA231Br-GFP brain metastases. Representative histologic sections of rat brains, intrastriatally injected with MDA231Br-GFP tumor cells, from the tumor core, tumor rim, and the contralateral striatum, with corresponding box and whisker plots of marker expression in each region. Data shown as median ± interquartile range. Sections were immunohistochemically stained (brown) for tumor cell marker vimentin (A), endothelial marker CD31 (B), cell adhesion molecule VCAM-1 (C), cell proliferation marker Ki67 (D), and two stemness markers: SOX2 (E) and nestin (F). Scale bar = 100 μm. *, P < 0.05; ***, P < 0.001; n = 8; post hoc Bonferroni multiple comparison test for tumor cell density and microvessel density, and post hoc Dunn test for VCAM-1 expression. Tumor core was delineated from the infiltrative border as indicated by the blue and red lines, respectively. Expression of Ki67, nestin and SOX2 has been normalized to tumor area. *, P < 0.05; ***, P < 0.001; matched Wilcoxon test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35312755), licensed under a CC-BY license. Not internally tested by R&D Systems.

Microvascular and tumor cell biology at the tumor rim in U87MG glioblastoma. Representative histologic sections of rat brains, intrastriatally injected with U87MG tumor cells, from the tumor core, tumor rim, and the contralateral striatum, with corresponding box and whisker plots of marker expression in each region. Data shown as median ± interquartile range. Sections were immunohistochemically stained (brown) for tumor cell marker vimentin (A), endothelial marker CD31 (B), cell adhesion molecule VCAM-1 (C), cell proliferation marker Ki67 (D), and two stemness markers: SOX2 (E) and nestin (F). Scale bar = 100 μm. *, P < 0.05; **, P < 0.01; ***, P < 0.001; n = 5; post hoc Bonferroni multiple comparison test for tumor cell density and microvessel density, and post hoc Dunn test for VCAM-1 expression. Tumor core was delineated from the infiltrative border as indicated by the blue and red lines, respectively. Expression of Ki67, nestin, and SOX2 has been normalized to tumor area. **, P < 0.01; matched Wilcoxon test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35312755), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry (paraffin-embedded) of Native Adult Rat lung tissue stained for Abca3 (red), CD31/PECAM-1 (green), and Dclk1 (cyan). Image from a verified customer review.

IF staining of human lung endothelial cells with cd31/pecam1. Image from a verified customer review.

Immunostaining analysis of angiogenic markers. (a) Immunohistochemistry (IHC) staining and semi‐quantitative analysis of CD31 in regenerated Achilles tendon of control, suture repair (SR), sodium alginate (SA), and bioactive glass (BG)/SA groups on Days 3, 7, 14, and 28 post‐surgery. Scale bar = 100 μm. T, tendon tissue; G, granulation tissue. Black arrows denote positive signals. Results of statistical analysis are presented as means ± SD; (n = 3; *p < 0.05). (b) Immunofluorescence (IF) staining of CD31 and alpha ‐SMA in regenerated Achilles tendon of SR, SA, and BG/SA groups on Days 7, 14, and 28 post‐surgery. Scale bar = 50 μm; Scale bar (zoom) = 20 μm Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36684098), licensed under a CC-BY license. Not internally tested by R&D Systems.

FGF-2 expression in NPC correlates with tumor vasculature.A Transcriptomic expression levels of angiogenic factors, including VEGFA, FGF2, PDGFB, EGF, ANGPT1, EPO in human KIRC tissues, COAD tissues, NPC tissues, STAD tissues, PAAD tissues, LUAD tissues, BRCA tissues, SKCM tissues, and their adjacent healthy tissues. The red line indicates the highest expression of FGF2 in AAD-resistant NPC and the lowest expression of FGF in AAD-sensitive CRC. B Transcriptomic expression levels of FGF2 in NPC tissues, COAD tissues, and their adjacent healthy tissues (sample number: control-NPC/NPC/control-COAD/COAD = 10/31/41/290). Data were extracted from datasets GSE12452 and TCGA. C Human rhinitis tissues and NPC tissues were collected and detected for histology (H&E), FGF-2, and CD31 expression levels. Scale bar in upper panel = 500 μm, scale bar in lower three panels = 50 μm. Quantification of FGF-2+ or CD31+ signals (n = 8 random fields per group). D QPCR quantification of FGF2 and CD31 expression in freshly collected rhinitis tissues and NPC tissues (rhinitis tissue, n = 5 samples; NPC tissue, n = 6 samples). E Correlation of FGF2 and CD31 transcriptomic expression levels of human NPCs and control rhinitis tissues (Rhinitis tissue, n = 5 samples; NPC tissue, n = 6 samples). *P < 0.05; ***P < 0.001. NS not significant. Data presented as mean ± SD. KIRC kidney renal clear cell carcinoma, COAD colon adenocarcinoma, STAD stomach adenocarcinoma, PAAD pancreatic adenocarcinoma, LUAD lung adenocarcinoma, BRCA breast invasive carcinoma, SKCM skin cutaneous melanoma. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35985991), licensed under a CC-BY license. Not internally tested by R&D Systems.

Increase in renal fibrosis in aging kidneys of Atg7flox/flox;Tie2-Cre+ mice. (A) Representative immunoblots and densitometry for the expression of Atg7. (B) Relative protein level of Atg7 (%). (C) Representative images of H&E staining from wild-type and Atg7flox/flox;Tie2-Cre+ mice. Scale bars, 100 μm. (D) Quantification of glomeruli diameters (µm). (E) Representative 3,3′-diaminobenzidine (DAB) staining for CD31. Scale bars, 50 μm. (F) Quantification of capillary lumen diameters (µm). (G) Representative immunoblots and densitometry for the expression of CD31. (H) Relative protein levels of CD31 (%). (I) Masson’s trichrome staining from wild-type and Atg7flox/flox;Tie2-Cre mice, showing the cortex and medulla with increased extracellular matrix deposition in the aging Atg7flox/flox;Tie2-Cre+ mouse. (J) Quantification of tubulointerstitial fibrosis (%). (K) Representative DAB staining for TGF-beta . (L) Quantification of TGF-beta positive areas (%). (M) Representative immunoblots and densitometry for expression of TGF-beta . (N) Relative protein level of TGF-beta (%). (O) Representative DAB staining for alpha -SMA. (P) Quantification of alpha -SMA-positive areas (%). (Q) Representative immunoblots and densitometry for expression of alpha -SMA. (R) Relative protein levels of alpha -SMA (%). Scale bars, 50 μm for cortex and 100 μm for medulla. Values are means ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001, ns: not significant. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/2076-3921/13/8/886), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3628U-100UG
Alexa Fluor 405	AF3628V-100UG
Alexa Fluor 488	FAB3628G-025
Alexa Fluor 488	FAB3628G-100
Alexa Fluor 532	AF3628X-100UG
Alexa Fluor 594	AF3628T-100UG
Alexa Fluor 647	AF3628R-100UG
Alexa Fluor 700	AF3628N-100UG
Alexa Fluor 750	AF3628S-100UG
Alexa Fluor Plus 405	AF3628AFP405-100UG
Alexa Fluor Plus 488	AF3628AFP488-100UG
Alexa Fluor Plus 555	AF3628AFP555-100UG
Alexa Fluor Plus 594	AF3628AFP594-100UG
Alexa Fluor Plus 647	AF3628AFP647-100UG
Alexa Fluor Plus 680	AF3628AFP680-100UG
Allophycocyanin	FAB3628A-100
Allophycocyanin	FAB3628A-025
Biotin	BAF3628
CoraFluor 1	AF3628CL1
DyLight 350	AF3628D
DyLight 405	AF3628E
DyLight 488	AF3628K
DyLight 550	AF3628L
DyLight 594	AF3628M
DyLight 650	AF3628W
DyLight 680	AF3628Y
DyLight 755	AF3628Z
FITC	AF3628F
HRP	AF3628H
Janelia Fluor 525	AF3628JF525
Janelia Fluor 549	AF3628I
Janelia Fluor 646	AF3628J
Janelia Fluor 669	AF3628JF669
PerCP	AF3628C
Phycoerythrin	FAB3628P-100
Phycoerythrin	FAB3628P-025
mFluor Violet 450 SE	AF3628MFV450
mFluor Violet 500 SE	AF3628MFV500
mFluor Violet 610 SE	AF3628MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3628-SP
Carrier Free	FAB3628P-100
Carrier Free	FAB3628P-025
Carrier Free	FAB3628G-100
Carrier Free	FAB3628G-025
Carrier Free	FAB3628A-100
Carrier Free	FAB3628A-025

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NB100-2284

CD31/PECAM-1 Antibody

(5 Reviews)

(151 Publications)

0.1 ml

NB100-2284

Staining of CD31/PECAM-1 in Rat Liver sample. IHC-P image submitted by a verified customer review.

Human lung adenocarcinoma using. Antibody at 1:100 with citrate epitope retrieval at pH 6.0.

Analysis in rat muscle blood vessels using. IHC-P image submitted by a verifed customer review.

Matrix-HA supplemented with strontium was implanted subcutaneously in mice, where immunochemistry analysis and histology occurred of the newly formed tissues. (C) Within each matrix: Matrix-HA, Matrix-8Sr-HA and Matrix-50Sr-HA, were indicated by immunostaining of CD31 for the newly formed tissues. (D) Number of vessels within each tissue was quantified using NDP view software. Unit is square mm. Immunostaining analysis of slides occurred for 2 samples per condition and 3 sections were analyzed per sample and per group of matrix. Citation: Ehret C, Aid-Launais R, Sagardoy T, Siadous R, Bareille R, Rey S, et al. (2017) Strontium-doped hydroxyapatite polysaccharide materials effect on ectopic bone formation. PLoS ONE 12(9): e0184663. //doi.org/10.1371/journal.pone.0184663

Imaging of HUVEC monolayer on glass substrate. Fixation with 4% PFA. Blocking with Goat Serum at 1:100. Incubation 2 hours at room temperature. ICC/IF image submitted by a verified customer review.

Analysis of canine kidney tissue using CD31/PECAM-1 antibody. Antibody was used at a 1:50 concentration in Casein in PBS and left at 4C overnight on paraffin embedded canine bladder tissue. HIER was performed in Tris/EDTA buffer, pH 9 for two hours at 75C. Image from verified customer review.

Analysis in pig SC cells using CD31/PECAM-1 Antibody (red). Blue color showing nucleus labeling. ICC/IF image submitted by a verified customer review.

CD31-PECAM-1-Antibody-Immunohistochemistry-NB100-2284-img0021.jpg

CD31 staining on E11.5 mouse Pharyngeal Mesoderm. Fixed with 4% PFA overnight. Blocked with 1% BSA. Primary antibody at 1:100. Secondary antibody at 1:1000, conjugated to Alexa Fluor 488. IHC-Fr image submitted by a verfied customer review.

Immunohistochemistry evaluation of CD31 expression. (a) Representative images of CD31 staining of vessels in the three groups of implanted matrices (scale bar = 100 µm). (b) Quantification of vessel density inside the implanted matrices at 5 weeks post implantation (n = 6; Average ± SD). NS and **denote Non Significant and p < 0.01, respectively.

Immunohistochemistry: Rabbit Polyclonal CD31/PECAM-1 Antibody [NB100-2284] - Staining of CD31 in human lung tissue. Nuclei in blue and CD31 (vessel) in green. Mouse anti-CD31 (1:100, overnight incubation at +4°C), followed by donkey anti-mouse 488 (1:400, 3h at RT). Image from a verified customer review.

Immunohistochemistry: CD31/PECAM-1 Antibody [NB100-2284] - Antibodies selected for characterization of stromal cells & vasculature in FFPE specimens are species-specific. (A, B) Labeling of a patient colon tumor (A) & Colo205 human cell line xenograft (B) with anti-human mitochondrial antibody (single arrow- tumor cells; double arrows- stroma). (C, D) Labeling of a patient pancreatic tumor (C) & Colo205 human cell line xenograft (D) with anti-human CD34. (E, F) Labeling of a patient lung tumor (E) & Colo205 xenograft (F) with anti-human CD31. (G, H) Labeling of a patient lung tumor (G) & Colo205 xenograft (H) with anti-mouse CD34. (bars = 50 μ). Image collected & cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-11-110), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: CD31/PECAM-1 Antibody [NB100-2284] - At the time of second passage, vessels in successfully growing xenografts of four different tumor types surveyed were of murine origin: A-C Colon; D-F Lung; G-I Pancreatic; J-L Renal Cell Carcinoma. For each tumor type, a representative section of an original patient specimen labeled with anti-huCD31 is shown (A, D, G, J). For each tumor, sections of the first passage xenografted tumor when it was resected are also shown; while no huCD31(+) vessels were identified in the xenografts (B, E, H, K), msCD34(+) vessels were abundant (C, F, I, L). (bars = 50 μ). Image collected & cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-11-110), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: CD31/PECAM-1 Antibody [NB100-2284] - Vascularization of an engrafted patient colon tumor. A patient colon tumor was implanted in a cohort of mice & vessel development was analyzed over 8 weeks. Vessels in the original patient specimen labeled strongly for huCD31 (A) & not for msCD34 (D). Representative sections showing loss of huCD31(+) vessels (B- 4 weeks, C- 7 weeks) & presence of msCD34(+) vessels (E- 4 weeks, F- 7 weeks) are shown. The graph (G) summarizes this process; huCD31(+) vessels were rapidly lost, & by one week, mouse vessels were the predominate vessels present in the colon tumors (no data for msCD34 at 2 weeks; bars = 100 μ). Image collected & cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-11-110), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: CD31/PECAM-1 Antibody [NB100-2284] - KCl pulse decreased membrane, but increased nuclear, detection of NHE1 in b.End3 endothelial cells.(A) Representative immunofluorescence images of bEnd.3 cells at two time-points after KCl or aCSF pulse. (B) Representative immunoblots of NHE1, alpha -tubulin, PECAM, & lamin B in cytosol, membrane, & nuclear fractions of bEnd.3 cells harvested at 5 min after KCl or aCSF pulse. (C = cytosol, M = membrane, N = nuclear) Values represent the mean ratio of NHE1 detection ± SEM (n = 11–12). (C) Representative immunoblots indicating NHE1 & alpha -tubulin as a loading control in whole cell lysate of bEnd.3 cells harvested at 5 min after KCl or aCSF pulse. Values represent the % of aCSF-treated relative expression ± SEM (n = 6). (D) Representative immunoblots of NHE1 & alpha -tubulin as a loading control in whole lysate of microvessels harvested at 90 min after cortical injection of KCl or aCSF. Values represent the % of naive relative expression ± SEM (n = 6). # denotes significantly different vs naïve (p<0.01), as assessed by one-way ANOVA (E) Intracellular pH during aCSF or KCl pulse. All data represent mean ± SEM (n = 45). (F) Extracellular pH during aCSF or KCl pulse. All data represent mean ± SEM (n = 6) in triplicate. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32469979), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody [NB100-2284] - Impact of T‐AuNPs on endothelial cells in vivo. Immunofluorescent staining for endothelial cells using an anti‐CD 31 antibody revealed an intact endothelial cell monolayer in the uninjured control carotid artery (N = 3). As expected post balloon angioplasty, there is no endothelial cell monolayer of the injured left carotid arteries in the injury alone, injury + T‐AuNP, & injury + S‐AuNP treatment conditions. Green indicates autofluorescence of the elastic lamina. Magenta indicates endothelial cells. Images obtained using 25× magnification with exposure time of 400 msec. TUNEL staining for apoptosis revealed no evidence of endothelial cell apoptosis in the uninjured control carotid arteries, with a prominent endothelial cell monolayer (white arrowhead). Mild apoptosis was noted in the media of balloon‐injured left carotid arteries, as expected. Black arrows indicate apoptotic cells. Representative images obtained using 40× magnification. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28242820), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: CD31/PECAM-1 Antibody [NB100-2284] - The growth of a patient mesothelioma xenograft was supported by development of a murine vascular network. A patient mesothelioma was implanted in a cohort of mice & monitored for tumor growth. Once tumors began to actively grow, representative tumors were resected at weekly intervals & analyzed for vessel content. Staining for huCD31 was prominent in the original patient specimen (A), much reduced at 4 weeks (B) & negligible at 9 weeks (C). In contrast, patient specimens were not stained for msCD34 (D), whereas at 4 weeks large numbers of vessels stained for msCD34 (E) & by 9 weeks, msCD34 labeled vessels were predominant (F). The graph (G) summarizes the loss of detectable human vessels & acquisition of murine vessels over a 9 week period. (bars = 100 μ). Image collected & cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-11-110), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody [NB100-2284] - TGF‐ beta 1 released by MSCs contributes to induced myofibroblast differentiation & granulation tissue formationA–C2.5 × 105 of TGF‐ beta 1 siRNA or control siRNA‐transfected AT‐MSCs were intradermally injected around each of CD18−/− murine wound. PBS mock injection served as negative control. Wound tissue was harvested at day 2, 5, & 7 post‐wounding for quantification of human TGF‐ beta 1 mRNA (A) at day 2 by qPCR, total TGF‐ beta 1 (B), & active TGF‐ beta 1 (C) protein at day 5 by ELISA. Data are expressed as mean ± SEM, n = 3 wounds per group, **P < 0.01, by one‐way ANOVA with Tukey's test.D–GExpression of alpha ‐SMA (D & E) & CD31 (F & G) at days 5 & 7 by immunostaining on tissue sections. The dashed lines indicate the border of the wound bed & epidermis or eschar. e, epidermis; es, eschar; wb, wound bed. Scale bars: 100 μm. Quantification data are expressed as mean ± SEM, n = 3 wounds per group, *P < 0.05, ***P < 0.001, by one‐way ANOVA with Tukey's test.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32080965), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Paraffin: Rabbit Polyclonal CD31/PECAM-1 Antibody [NB100-2284] - CD31 positive vessels in a canine splenic hemangiosarcoma.Tissue slides were loaded into automated research stainer, dewaxed and pretreated with EDTA-based epitope retrieval ER2 solution for 20 mins at 100°C. After a peroxide incubation for 5 min, the rabbit antibody against CD31 (NB100-2284) (1:100) was incubated for 60 mins at room temperature followed by Bond Polymer (anti-rabbit HRP) incubation for 8 min at room temperature. Mixed DAB reagent (Polymer Refine Detection Kit) was incubated for 10 mins, and Hematoxylin (Refine Detection Kit) counterstaining for 10 mins. After staining, sample slides were washed in water, dehydrated using ethanol gradient (70%, 90%, 100%), washed three times, and mounted in mounting medium. Image from a verified customer review.

Reactivity:

Hu, Mu, Rt, Po, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Ca

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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AF806

CD31/PECAM-1 Antibody [Unconjugated]

(75 Publications)

100 μg

AF806

HAF016). A specific band was detected for CD31/PECAM-1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (<a class=

HAF016). A specific band was detected for CD31/PECAM-1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for CD31/PECAM-1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

CD31/PECAM-1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using 10 µg/mL Sheep Anti-Human CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # <a class=

NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

HAF016) in Milk-free Antibody Diluent (Catalog # 043-524). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) tested at 10 µg/ml (blue line) and 50 µg/ml (green line) in the HUVEC human umbilical vein endothelial cells." class="big_lightbox">

<b>Left:</b> Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.1 mg/ml. A specific band was detected for CD31/PECAM‑1 at approximately 155 kDa (as indicated) using both 10 µg/ml and 50 µg/ml of Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016) in Milk-free Antibody Diluent (Catalog # 043-524). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) tested at 10 µg/ml (blue line) and 50 µg/ml (green line) in the HUVEC human umbilical vein endothelial cells." class="big_thumb" />

NL010) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

<b>Genetic Strategies Validation. </b>CD31/PECAM‑1 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line but is not detected in CD31/PECAM‑1 knockout (KO) THP‑1 human cell line using Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; <a class=

NL010) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells and sinusoids. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells and sinusoids. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Expression pattern of integrin receptors and type IV collagen in normal pancreatic tissue. A. H&E staining of normal exocrine pancreas with acini structures and a duct in the insert. The area shown is representative for all panels in B. B. Merged immunofluorescence staining of integrin alpha 1, alpha 2, and beta 1 (in red), and type IV collagen (green in upper row), or the endothelial marker CD31 (green in lower row). Type IV collagen is present in the vascular-, ductal-, and acini-BMs. Integrin alpha 1 is only expressed in endothelial cells and integrin alpha 2 only in the ductal epithelium. Integrin beta 1 is found in the ductal-, endothelial-, and at the base of the acini-cells. Arrows indicate examples of colocalization. Magnification x40 in all panels. Cell nuclei are stained by DAPI (in blue). Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-13-154), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF806U-100UG
Alexa Fluor 405	AF806V-100UG
Alexa Fluor 488	FAB806G-100
Alexa Fluor 532	AF806X-100UG
Alexa Fluor 594	AF806T-100UG
Alexa Fluor 647	AF806R-100UG
Alexa Fluor 700	AF806N-100UG
Alexa Fluor 750	AF806S-100UG
Alexa Fluor Plus 405	AF806AFP405-100UG
Alexa Fluor Plus 488	AF806AFP488-100UG
Alexa Fluor Plus 555	AF806AFP555-100UG
Alexa Fluor Plus 594	AF806AFP594-100UG
Alexa Fluor Plus 647	AF806AFP647-100UG
Alexa Fluor Plus 680	AF806AFP680-100UG
Allophycocyanin	AF806A
Biotin	BAF806
CoraFluor 1	AF806CL1
DyLight 350	AF806D
DyLight 405	AF806E
DyLight 488	AF806K
DyLight 550	AF806L
DyLight 594	AF806M
DyLight 650	AF806W
DyLight 680	AF806Y
DyLight 755	AF806Z
FITC	AF806F
HRP	AF806H
Janelia Fluor 525	AF806JF525
Janelia Fluor 549	AF806I
Janelia Fluor 646	AF806J
Janelia Fluor 669	AF806JF669
PE	AF806P
PerCP	AF806C
mFluor Violet 450 SE	AF806MFV450
mFluor Violet 500 SE	AF806MFV500
mFluor Violet 610 SE	AF806MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF806-SP
Carrier Free	FAB806G-100

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NB600-562

CD31/PECAM-1 Antibody (JC/70A) - BSA Free

(4 Reviews)

(54 Publications)

0.1 ml

NB600-562

CD31/PECAM-1 was detected in immersion fixed THP-1 (red, positive) but not THP-1 KO (negative) cells using 8 ug/mL Mouse Anti-Human CD31/PECAM-1 (JC/70A) Monoclonal Antibody (Catalog # NB600-562) for 3 hours at room temperature. Cells were stained with the NorthernLights(TM) 557-conjugated Donkey anti-Mouse IgG Secondary Antibody (red, Catalog # NL007)) and and counterstained with DAPI (blue).

<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Formalin-fixed paraffin-embedded tissue sections of human metastatic tonsil were probed for CD31 mRNA (ACD RNAScope probe, catalog # 487381; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processes for immunohistochemistry using mouse monoclonal (NB600-562) at 1:25 dilution for 1 hour at room temperature followed by incubation with the anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).

Rabbit Heart, CD31 Stained Red. Image from verified customer review.

A cell surface stain was performed on HUVEC cells with ( NB600-562, blue) along with a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1:100 for 20 minutes at RT. (Panel A). A negative control (HeLa cells) was also stained to ensure antibody specificity (Panel B).

CD31-PECAM-1-Antibody-JC-70A-Immunohistochemistry-NB600-562-img0022.jpg

Analysis of CD31/PECAM1 in human spleen using DAB with hematoxylin counterstain.

Analysis of CD31/PECAM1 expression in 1) Jurkat whole cell lysate, 2) human platelet lysate and 3) U937 whole cell lysate.

Comparison of the expression of NMDAR1 on vessels and EndoMT markers in the urinary bladder.Biopsy specimens of frozen sections from the urinary bladders of (A, C, E) normal control subjects and (B, D, F) KC patients. (A, B) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) and NMDAR1 (red); DAPI was used as a nuclear stain (blue); co-expression of CD31 and NMDAR1 was evident in two groups (yellow, denoted by white arrows). (CF) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) and two mesenchymal markers: FSP1 (red, in panels C and D) and -SMA (red, in panels E and F); DAPI was used as a nuclear stain (blue); co-expression of CD31 and FSP1 or -SMA was evident in three groups (yellow, denoted by white arrows) (white bars: 50 m). Microvascular Injury in Ketamine-Induced Bladder Dysfunction. <i>PLoS One</i> (2016)

A surface stain was performed on Jurkat cells with the Dylight 550 conjugate of CD31/PECAM-1 Antibody (JC/70A) (NB600-562R, blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to DyLight 550.

HUVEC cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with CD31/PECAM-1 Antibody (JC/70A) at 2 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Imaging of Feline Endothelial cells with antibody dilution of 1:25. CD31/PECAM-1 (Red), DAPI (Blue). This image was submitted via customer Review.

FFPE human tonsil stained with CD31/PECAM-1 Antibody (JC/70A).

A cell surface stain was performed on Jurkat cells with the Alexa Fluor 488 conjugate of CD31/PECAM-1 Antibody (JC/70A) (NB600-562AF488, blue) along with a matched isotype control. Cells were incubated in an antibody dilution of 10 ug/ml for 20 minutes at RT. Both antibodies were conjugated to Alexa Fluor 488.

A cell surface stain was performed on THP-1 cells with the Alexa Fluor 488 conjugate of CD31/PECAM-1 Antibody (JC/70A) NB600-562AF488 (blue) along with a matched isotype control. Cells were incubated in an antibody dilution of 5 ug/ml for 20 minutes at RT. Both antibodies were conjugated to Alexa Fluor 488.

A surface stain was performed on THP-1 cells with the Alexa Fluor 647 conjugate of CD31/PECAM-1 Antibody (JC/70A) (NB600-562AF647, blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (JC/70A) - BSA Free [NB600-562] - Immunohistochemial comparison of the expression of NMDAR1 on vessels & EndoMT markers in the urinary bladder.Biopsy specimens of frozen sections from the urinary bladders of (A, C, E) normal control subjects & (B, D, F) KC patients. (A, B) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) & NMDAR1 (red); DAPI was used as a nuclear stain (blue); co-expression of CD31 & NMDAR1 was evident in two groups (yellow, denoted by white arrows). (C–F) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) & two mesenchymal markers: FSP1 (red, in panels C & D) & alpha -SMA (red, in panels E & F); DAPI was used as a nuclear stain (blue); co-expression of CD31 & FSP1 or alpha -SMA was evident in three groups (yellow, denoted by white arrows) (white bars: 50 μm). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27529746), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

IF staining of IPAH patient CD31/PECAM-1 lung slice. Image from a verified customer review.

Reactivity:

Hu, Mu, Fe, Rb

Species Glossary

Reactivity: Hu, Mu, Fe, Rb

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-562AF350
Alexa Fluor 405	NB600-562AF405
Alexa Fluor 488	NB600-562AF488
Alexa Fluor 532	NB600-562AF532
Alexa Fluor 594	NB600-562AF594
Alexa Fluor 647	NB600-562AF647
Alexa Fluor 700	NB600-562AF700
Alexa Fluor 750	NB600-562AF750
Alexa Fluor Plus 405	NB600-562AFP405
Alexa Fluor Plus 488	NB600-562AFP488
Alexa Fluor Plus 555	NB600-562AFP555
Alexa Fluor Plus 594	NB600-562AFP594
Alexa Fluor Plus 647	NB600-562AFP647
Alexa Fluor Plus 680	NB600-562AFP680
Allophycocyanin	NB600-562APC
Biotin	NB600-562B
CoraFluor 1	NB600-562CL1
DyLight 350	NB600-562UV
DyLight 405	NB600-562V
DyLight 488	NB600-562G
DyLight 550	NB600-562R
DyLight 594	NB600-562DL594
DyLight 650	NB600-562C
DyLight 680	NB600-562FR
DyLight 755	NB600-562IR
FITC	NB600-562F
HRP	NB600-562H
Janelia Fluor 525	NB600-562JF525
Janelia Fluor 549	NB600-562JF549
Janelia Fluor 646	NB600-562JF646
Janelia Fluor 669	NB600-562JF669
PE	NB600-562PE
PerCP	NB600-562PCP
mFluor Violet 450 SE	NB600-562MFV450
mFluor Violet 500 SE	NB600-562MFV500
mFluor Violet 610 SE	NB600-562MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB600-562SS
Azide and BSA Free	NBP2-80641

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BBA7

CD31/PECAM-1 Antibody (9G11) [Unconjugated]

(74 Publications)

100 μg

BBA7

HAF018). A specific band was detected for CD31/PECAM-1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CD31/PECAM-1 Monoclonal Antibody (Catalog # BBA7) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (<a class=

HAF018). A specific band was detected for CD31/PECAM-1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CD31/PECAM-1 Monoclonal Antibody (Catalog # BBA7) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for CD31/PECAM-1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

CD31/PECAM-1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using 10 µg/mL Mouse Anti-Human CD31/PECAM-1 Monoclonal Antibody (Catalog # BBA7) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; <a class=

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of Artery in Human Liver using Mouse Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # BBA7) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Characterization&functional analysis of expanded AEPCs compared with HUVECs. All the experiments performed using AEPCs&HUVECs at passage number 5. (A) Fluorescent immunocytochemistry of the expression profiles of endothelial cell-specific markers, CD31, vWF,&the binding capacity of isolectin-B4, in AEPCs&HUVECs. Nuclear staining was assessed by 4′,6-diamidino-2-phenylindole (DAPI). Photographs taken using a confocal microscope (OLYMPUS, FV1000 with a CCD camera DP71&an objective lens UPlanFL N 40×/1.30 oil; 400× magnification) as a plane image. Bars represent 100 µm. The graphs show the %age of endothelial marker-positive cells among DAPI-positive cells. The data represent two independent experiments (2 donors),&%ages determined from cultivations performed in triplicate. The bars represent means ± SD (n = 6, **P < 0.01, two-tailed unpaired t-test). Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35110646), licensed under a CC-BY license. Not internally tested by R&D Systems.

IF staining of CD31/PECAM-1 in human lung tissue. Image from a verified customer review.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	BBA7U-100UG
Alexa Fluor 405	BBA7V-100UG
Alexa Fluor 488	BBA7G-100UG
Alexa Fluor 532	BBA7X-100UG
Alexa Fluor 594	BBA7T-100UG
Alexa Fluor 647	BBA7R-100UG
Alexa Fluor 700	FAB3567N-100
Alexa Fluor 700	FAB3567N-025
Alexa Fluor 750	BBA7S-100UG
Alexa Fluor Plus 405	BBA7AFP405-100UG
Alexa Fluor Plus 488	BBA7AFP488-100UG
Alexa Fluor Plus 555	BBA7AFP555-100UG
Alexa Fluor Plus 594	BBA7AFP594-100UG
Alexa Fluor Plus 647	BBA7AFP647-100UG
Alexa Fluor Plus 680	BBA7AFP680-100UG
Allophycocyanin	FAB3567A-025
Allophycocyanin	FAB3567A-100
Biotin	BAM3567
CoraFluor 1	BBA7CL1
DyLight 350	BBA7D
DyLight 405	BBA7E
DyLight 488	BBA7K
DyLight 550	BBA7L
DyLight 594	BBA7M
DyLight 650	BBA7W
DyLight 680	BBA7Y
DyLight 755	BBA7Z
Fluorescein	FAB3567F-025
Fluorescein	FAB3567F-100
HRP	BBA7H
Janelia Fluor 525	BBA7JF525
Janelia Fluor 549	BBA7I
Janelia Fluor 646	BBA7J
Janelia Fluor 669	BBA7JF669
PerCP (Peridinin-chlorophyll Protein Complex)	FAB3567C-100
PerCP (Peridinin-chlorophyll Protein Complex)	FAB3567C-025
Phycoerythrin	FAB3567P-100
Phycoerythrin	FAB3567P-025
mFluor Violet 450 SE	BBA7MFV450
mFluor Violet 500 SE	BBA7MFV500
mFluor Violet 610 SE	BBA7MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB3567P-100
Carrier Free	FAB3567P-025
Carrier Free	FAB3567F-100
Carrier Free	FAB3567F-025
Carrier Free	FAB3567A-100
Carrier Free	FAB3567A-025
Carrier Free	FAB3567N-100
Carrier Free	FAB3567N-025
Carrier Free	FAB3567C-100
Carrier Free	FAB3567C-025

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NB100-64796

CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free

(1 Review)

(61 Publications)

0.25 mg

NB100-64796

Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (TLD-3A12) [NB100-64796] - Analysis of CD31 in 4th day cutaneous wound section of diabetic rat using CD31/PECAM-1 Antibody (TLD-3A12). Image from verified customer review.

CD31-PECAM-1-Antibody-TLD-3A12-Immunohistochemistry-Paraffin-NB100-64796-img0014.jpg

(a) HE staining for wound tissues of different groups of rats (magnification ×40). (b) IHC analysis for the expression of CD31 of wound tissues of different groups of rats (magnification ×200). n = 6, ∗∗P < 0.01.

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-64796AF350
Alexa Fluor 405	NB100-64796AF405
Alexa Fluor 488	NB100-64796AF488
Alexa Fluor 532	NB100-64796AF532
Alexa Fluor 594	NB100-64796AF594
Alexa Fluor 647	NB100-64796AF647
Alexa Fluor 700	NB100-64796AF700
Alexa Fluor 750	NB100-64796AF750
Allophycocyanin	NB100-64796APC
Biotin	NB100-63701
Biotin	NB100-64796B
CoraFluor 1	NB100-64796CL1
DyLight 350	NB100-64796UV
DyLight 405	NB100-64796V
DyLight 488	NB100-64796G
DyLight 550	NB100-64796R
DyLight 594	NB100-64796DL594
DyLight 650	NB100-64796C
DyLight 680	NB100-64796FR
DyLight 755	NB100-64796IR
FITC	NB100-63703-100ug
FITC	NB100-64796F
HRP	NB100-64796H
Janelia Fluor 525	NB100-64796JF525
Janelia Fluor 549	NB100-64796JF549
Janelia Fluor 646	NB100-64796JF646
Janelia Fluor 669	NB100-64796JF669
PE	NB100-64796PE
PerCP	NB100-64796PCP
mFluor Violet 450 SE	NB100-64796MFV450
mFluor Violet 500 SE	NB100-64796MFV500
mFluor Violet 610 SE	NB100-64796MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-64796SS

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NB600-1475

CD31/PECAM-1 Antibody (MEC13.3) - BSA Free

(2 Reviews)

(31 Publications)

0.1 ml

NB600-1475

Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on WEHI-3 Cells with CD31/PECAM-1 Antibody (MEC13.3) (NB600-1475, blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature, followed by rat F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0113, R&D Systems).

Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - C57BL/6 mouse splenocytes stained with purified CD31/PECAM-1 Antibody (MEC13.3), followed by anti-rat IgG FITC

Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - MS1 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with at 5.0 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - was tested in Wehi-3 cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

CD31-PECAM-1-Antibody-MEC13-3-Immunohistochemistry-Paraffin-NB600-1475-img0015.jpg

Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on MS1 cells with CD31/PECAM-1 [MEC13.3] Antibody NB600-1475R (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.

Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - Staining of mouse CD31 in non-small cell lung cancer tumor sections from a human NSCLC tumor-bearing mouse. This image was submitted via customer Review.

Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on WEHI-3 Cells with CD31 (MEC13.3) antibody NB600-1475 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature, followed by rat F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0113, R&D Systems).

Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on MS-1 cells with CD31/PECAM-1 Antibody (MEC13.3) (NB600-1475AF647, blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on MS1 cells with CD31/PECAM-1 [MEC13.3] Antibody NB600-1475DL594 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 594.

Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on MS1 cells with CD31/PECAM-1 [MEC13.3] Antibody NB600-1475G (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.

Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475] - (A) Representative Western blots depicting phosphorylated JAK3 (pJAK3), total JAK3, & corresponding beta -actin levels in the ipsilateral (CXI) versus the contralateral (CXC) cortex at 24 h in sham & mice subjected to permanent middle cerebral artery occlusion (pMCAO). (B,C) Quantified pJAK3 & JAK3 values normalized to beta -actin in the cortex of sham & mice subjected to stroke. Both pJAK3 & JAK3 are significantly increased in the ipsilateral cortex of stroked animals (*P < 0.05 & **P < 0.01 with respect to sham ipsilateral/contralateral & stroke contralateral; sham, n = 5; stroke, n = 13). Data were analyzed using a two-way ANOVA (ipsilateral/contralateral or sham/stroke) with Bonferroni post-test. (D) Diagram showing peri-infarct brain region from which images were taken (n = 3). Scale bar is 10 µm, & magnification is 60×. (E) JAK3 colocalized with microglia/macrophages labeled with Iba-1. (F) JAK3 colocalized with neurons labeled with NeuN. (G) JAK3 colocalized with endothelial cells labeled with CD31. (H) JAK3 is not colocalized with astrocytes labeled with GFAP. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28790974), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

CD31/PECAM-1 (MEC13.3) was detected in immersion fixed MS1 mouse pancreas/Islet of Langerhans endothelial cell line using Rat anti-CD31/PECAM-1 (MEC13.3) Protein-G purified Monoclonal Antibody conjugated to DyLight 550 (Catalog # NB600-1475R) (red) at 2 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-1475AF350
Alexa Fluor 405	NB600-1475AF405
Alexa Fluor 488	NB600-1475AF488
Alexa Fluor 532	NB600-1475AF532
Alexa Fluor 594	NB600-1475AF594
Alexa Fluor 647	NB600-1475AF647
Alexa Fluor 700	NB600-1475AF700
Alexa Fluor 750	NB600-1475AF750
Alexa Fluor Plus 405	NB600-1475AFP405
Alexa Fluor Plus 488	NB600-1475AFP488
Alexa Fluor Plus 555	NB600-1475AFP555
Alexa Fluor Plus 594	NB600-1475AFP594
Alexa Fluor Plus 647	NB600-1475AFP647
Alexa Fluor Plus 680	NB600-1475AFP680
Allophycocyanin	NB600-1475APC
Biotin	NB600-1475B
CoraFluor 1	NB600-1475CL1
DyLight 350	NB600-1475UV
DyLight 405	NB600-1475V
DyLight 488	NB600-1475G
DyLight 550	NB600-1475R
DyLight 594	NB600-1475DL594
DyLight 650	NB600-1475C
DyLight 680	NB600-1475FR
DyLight 755	NB600-1475IR
FITC	NB600-1475F
HRP	NB600-1475H
Janelia Fluor 525	NB600-1475JF525
Janelia Fluor 549	NB600-1475JF549
Janelia Fluor 646	NB600-1475JF646
Janelia Fluor 669	NB600-1475JF669
PE	NB600-1475PE
PerCP	NB600-1475PCP
mFluor Violet 450 SE	NB600-1475MFV450
mFluor Violet 500 SE	NB600-1475MFV500
mFluor Violet 610 SE	NB600-1475MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB600-1475SS
Azide and BSA Free	NBP2-80640

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NB100-1642

CD31/PECAM-1 Antibody (MEC 7.46) - BSA Free

(29 Publications)

0.1 ml

NB100-1642

CD31-PECAM-1-Antibody-MEC-7-46-Immunohistochemistry-NB100-1642-img0011.jpg

Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - MS1 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with at 5.0 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

<b>Genetic Strategies Validation. </b>Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Frozen mouse lung tissues of WT B6 and IL1R--/- (knockout) mice were blocked with 1% BSA in PBS and were double stained with Alex647-conjugated CD31/PECAM-1 antibody (MEC 7.46) and PE-conjugated F4/80 for 2 hours at the room temperature. Image using the Alexa Fluor 647 format of this antibody.

Flow Cytometry: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - A surface stain was performed on MS1 cells with CD31/PECAM-1 [MEC 7.46] Antibody NB100-1642AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

CD31-PECAM-1-Antibody-MEC-7-46-Immunohistochemistry-NB100-1642-img0010.jpg

Flow Cytometry: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - CD31/PECAM-1 Antibody (MEC 7.46) was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).

CD31/PECAM-1 (MEC 7.46) was detected in immersion fixed MS1 mouse pancreas/Islet of Langerhans endothelial cell line using Rat anti-CD31/PECAM-1 (MEC 7.46) Protein-G purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NB100-1642AF647) (light blue) at 2 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry for vessel staining (CD31) and beta -amyloid comparing 13- and 24-month old arcA beta mice. Overviews (scale bars = 200 μm) of the corpus callosum (CC), thalamus (TH), and cerebellum (CB). Insets show the regions with higher magnification (scale bar = 50 μm). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26834622), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry for vessel staining (CD31) and beta -amyloid comparing 13- and 24-month old non-transgenic littermates. Overviews (scale bars = 200 μm) of the corpus callosum (CC), thalamus (TH), and cerebellum (CB). Insets show the regions with higher magnification (scale bar = 50 μm). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26834622), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-1642AF350
Alexa Fluor 405	NB100-1642AF405
Alexa Fluor 488	NB100-1642AF488
Alexa Fluor 532	NB100-1642AF532
Alexa Fluor 594	NB100-1642AF594
Alexa Fluor 647	NB100-1642AF647
Alexa Fluor 700	NB100-1642AF700
Alexa Fluor 750	NB100-1642AF750
Alexa Fluor Plus 405	NB100-1642AFP405
Alexa Fluor Plus 488	NB100-1642AFP488
Alexa Fluor Plus 555	NB100-1642AFP555
Alexa Fluor Plus 594	NB100-1642AFP594
Alexa Fluor Plus 647	NB100-1642AFP647
Alexa Fluor Plus 680	NB100-1642AFP680
Allophycocyanin	NB100-1642APC
Biotin	NB100-1642B
CoraFluor 1	NB100-1642CL1
DyLight 350	NB100-1642UV
DyLight 405	NB100-1642V
DyLight 488	NB100-1642G
DyLight 550	NB100-1642R
DyLight 594	NB100-1642DL594
DyLight 650	NB100-1642C
DyLight 680	NB100-1642FR
DyLight 755	NB100-1642IR
FITC	NB100-1642F
HRP	NB100-1642H
Janelia Fluor 525	NB100-1642JF525
Janelia Fluor 549	NB100-1642JF549
Janelia Fluor 646	NB100-1642JF646
Janelia Fluor 669	NB100-1642JF669
PE	NB100-1642PE
PerCP	NB100-1642PCP
mFluor Violet 450 SE	NB100-1642MFV450
mFluor Violet 500 SE	NB100-1642MFV500
mFluor Violet 610 SE	NB100-1642MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-1642SS
Azide and BSA Free	NBP2-80639

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NBP1-71663

CD31/PECAM-1 Antibody - BSA Free

(2 Reviews)

(17 Publications)

0.1 ml

NBP1-71663

<b>Biological Strategies Validation. </b>CD31-PECAM-1-Antibody-Immunohistochemistry-NBP1-71663-img0012.jpg

CD31-PECAM-1-Antibody-Western-Blot-NBP1-71663-img0013.jpg

Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody [NBP1-71663] - HUVEC cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with CD31/PECAM-1 Antibody at 5 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: CD31/PECAM-1 Antibody [NBP1-71663] - CD31/PECAM-1 Antibody was tested in WeHi cells with Dylight 488 (green) alongside a matched isotype control (black).

Western Blot: CD31/PECAM-1 Antibody [NBP1-71663] - Detection of CD31 in HUVEC cell lysates.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-71663AF350
Alexa Fluor 405	NBP1-71663AF405
Alexa Fluor 488	NBP1-71663AF488
Alexa Fluor 532	NBP1-71663AF532
Alexa Fluor 594	NBP1-71663AF594
Alexa Fluor 647	NBP1-71663AF647
Alexa Fluor 700	NBP1-71663AF700
Alexa Fluor 750	NBP1-71663AF750
Alexa Fluor Plus 405	NBP1-71663AFP405
Alexa Fluor Plus 488	NBP1-71663AFP488
Alexa Fluor Plus 555	NBP1-71663AFP555
Alexa Fluor Plus 594	NBP1-71663AFP594
Alexa Fluor Plus 647	NBP1-71663AFP647
Alexa Fluor Plus 680	NBP1-71663AFP680
Allophycocyanin	NBP1-71663APC
Biotin	NBP1-71663B
CoraFluor 1	NBP1-71663CL1
DyLight 350	NBP1-71663UV
DyLight 405	NBP1-71663V
DyLight 488	NBP1-71663G
DyLight 550	NBP1-71663R
DyLight 594	NBP1-71663DL594
DyLight 650	NBP1-71663C
DyLight 680	NBP1-71663FR
DyLight 755	NBP1-71663IR
FITC	NBP1-71663F
HRP	NBP1-71663H
Janelia Fluor 525	NBP1-71663JF525
Janelia Fluor 549	NBP1-71663JF549
Janelia Fluor 646	NBP1-71663JF646
Janelia Fluor 669	NBP1-71663JF669
PE	NBP1-71663PE
PerCP	NBP1-71663PCP
mFluor Violet 450 SE	NBP1-71663MFV450
mFluor Violet 500 SE	NBP1-71663MFV500
mFluor Violet 610 SE	NBP1-71663MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-71663SS

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NBP2-44342

CD31/PECAM-1 Antibody (C31.3 + C31.7 + C31.10)

(11 Publications)

0.1 mg

NBP2-44342

Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.3 + C31.7 + C31.10) [NBP2-44342] - Colon Carcinoma stained with CD31/PECAM-1 Antibody (C31.3 + C31.7 + C31.10).

Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.3 + C31.7 + C31.10) [NBP2-44342] - Cholangiocellular carcinoma (liver cancer tissue) stained with ATTO550 conjugated CD31 antibody. Image from verified customer review.

Reactivity:

Hu, Rt, Pm-Cm, Rb

Species Glossary

Reactivity: Hu, Rt, Pm-Cm, Rb

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-47785AF350
Alexa Fluor 405	NBP2-47785AF405
Alexa Fluor 488	NBP2-47785AF488
Alexa Fluor 532	NBP2-47785AF532
Alexa Fluor 594	NBP2-47785AF594
Alexa Fluor 647	NBP2-47785AF647
Alexa Fluor 700	NBP2-47785AF700
Alexa Fluor 750	NBP2-47785AF750
Alexa Fluor Plus 405	NBP2-47785AFP405
Alexa Fluor Plus 488	NBP2-47785AFP488
Alexa Fluor Plus 555	NBP2-47785AFP555
Alexa Fluor Plus 594	NBP2-47785AFP594
Alexa Fluor Plus 647	NBP2-47785AFP647
Alexa Fluor Plus 680	NBP2-47785AFP680
Allophycocyanin	NBP2-47785APC
Biotin	NBP2-47785B
CoraFluor 1	NBP2-47785CL1
DyLight 350	NBP2-47785UV
DyLight 405	NBP2-47785V
DyLight 488	NBP2-47785G
DyLight 550	NBP2-47785R
DyLight 594	NBP2-47785DL594
DyLight 650	NBP2-47785C
DyLight 680	NBP2-47785FR
DyLight 755	NBP2-47785IR
FITC	NBP2-47785F
HRP	NBP2-47785H
Janelia Fluor 525	NBP2-47785JF525
Janelia Fluor 549	NBP2-47785JF549
Janelia Fluor 646	NBP2-47785JF646
Janelia Fluor 669	NBP2-47785JF669
PE	NBP2-47785PE

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-47785-0.1mg
IHC-Prediluted	NBP2-48331

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NBP2-15202

CD31/PECAM-1 Antibody (C31.3)

(7 Publications)

0.1 mg

NBP2-15202

Western Blot: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Western Blot Analysis of human Jurkat cell lysate using CD31/PECAM-1 Antibody (C31.3).

Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Immunofluorescence Analysis of PFA-fixed Jurkat cells labeled with CD31/PECAM-1 Antibody (C31.3) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)

Flow Cytometry: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Flow Cytometric analysis of human CD31 on Jurkat cells. Black: cells alone; grey: Isotype Control; Green: AF488-labeled CD31/PECAM-1 Antibody (C31.3).

Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Staining with CD31/PECAM-1 Antibody (C31.3) [Alexa Fluor® 647] (NBP2-33154AF647) using GE Cell DIVE (20x) on Human Rheumatoid Arthritis synovium (10 ug/ml). Image from verified customer review.

PFKFB3 expression is increased in advanced atherosclerotic plaques and correlates with CD68+ macrophages and necrotic core area. (A,B) Increased expression of PFKFB3 (green) in atheromatous plaques compared with fibrous carotid plaques. n = 10 for fibrous and fibroatheromatous plaques, n = 8 for atheromatous plaques; one-way ANOVA, P = 0.0078 for fibrous vs. atheromatous plaques. Scale bar 50 μm. (A,C) CD68+ macrophages (red) increased in atheromatous carotid plaques. n = 10 for fibrous and fibroatheromatous plaques, n = 8 for atheromatous plaques; one-way ANOVA, P = 0.0008 for fibrous vs. atheromatous plaques. Scale bar 50 μm (D) PFKFB3 expression and CD68 expression correlated significantly. n = 28; linear regression analysis, R2 = 0.7456, P < 0.0001. (E) Human coronary atherosclerotic advanced plaques showed increased PFKFB3 expression compared to initial plaques. n = 4 for initial vs. n = 5 for advanced plaques; two-tailed unpaired Mann-Whitney, P = 0.0317. Scale bar 200 μm. (F) PFKFB3 expression positively correlated with necrotic core area. n = 9; Linear regression analysis R2 = 0.4861, P = 0.0368. (G) Immunohistochemistry of PFKFB3+ co-staining with CD68+ macrophages; Scale bar 100 μm, (H) PECAM+ endothelial cells; Scale bar 100 μm, (I) CD3+ T cells; Scale bar 100 μm, and (J) and alpha smooth muscle actin ( alpha SMA)+ vascular smooth muscle cells (K); Scale bar 100 μm. Data are shown as mean +/- standard error of the mean. *P < 0.05, **P ≤ 0.005, ***P < 0.0005. PFKFB3, 6-phophofructo-2-kinase/fructose-2,6-biphosphatase 3; PECAM, platelet endothelial cell adhesion molecule; alpha SMA. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33282864), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

ABX-133A

Alt Catalog# ABX-133A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-33154AF350
Alexa Fluor 405	NBP2-33154AF405
Alexa Fluor 488	NBP2-33154AF488
Alexa Fluor 532	NBP2-33154AF532
Alexa Fluor 594	NBP2-33154AF594
Alexa Fluor 647	NBP2-33154AF647
Alexa Fluor 700	NBP2-33154AF700
Alexa Fluor 750	NBP2-33154AF750
Alexa Fluor Plus 405	NBP2-33154AFP405
Alexa Fluor Plus 488	NBP2-33154AFP488
Alexa Fluor Plus 555	NBP2-33154AFP555
Alexa Fluor Plus 594	NBP2-33154AFP594
Alexa Fluor Plus 647	NBP2-33154AFP647
Alexa Fluor Plus 680	NBP2-33154AFP680
Allophycocyanin	NBP2-33154APC
Biotin	NBP2-33154B
CoraFluor 1	NBP2-33154CL1
DyLight 350	NBP2-33154UV
DyLight 405	NBP2-33154V
DyLight 488	NBP2-33154G
DyLight 550	NBP2-33154R
DyLight 594	NBP2-33154DL594
DyLight 650	NBP2-33154C
DyLight 680	NBP2-33154FR
DyLight 755	NBP2-33154IR
FITC	NBP2-33154F
HRP	NBP2-33154H
Janelia Fluor 525	NBP2-33154JF525
Janelia Fluor 549	NBP2-33154JF549
Janelia Fluor 646	NBP2-33154JF646
Janelia Fluor 669	NBP2-33154JF669
PE	NBP2-33154PE
PerCP	NBP2-33154PCP
mFluor Violet 450 SE	NBP2-33154MFV450
mFluor Violet 500 SE	NBP2-33154MFV500
mFluor Violet 610 SE	NBP2-33154MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-33154
IHC-Prediluted	NBP2-44334

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NB100-65900

CD31/PECAM-1 Antibody (CO.3E1D4) - BSA Free

(6 Publications)

0.1 mg

NB100-65900

Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (CO.3E-1D4) [NB100-65900] - Bovine Lung stained with CD31 (green) and DAPI counterstain. Image provided courtesy of Hala Nijmeh, PhD and the PI, Evgenia V. Gerasimovskaya, PhD. Section of Critical Care Medicine, Department of Pediatrics, University of Colorado Anschutz Medical Center.

Reactivity:

Sh

Species Glossary

Reactivity: Sh

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-65900AF350
Alexa Fluor 405	NB100-65900AF405
Alexa Fluor 488	NB100-65900AF488
Alexa Fluor 532	NB100-65900AF532
Alexa Fluor 594	NB100-65900AF594
Alexa Fluor 647	NB100-65900AF647
Alexa Fluor 700	NB100-65900AF700
Alexa Fluor 750	NB100-65900AF750
Alexa Fluor Plus 405	NB100-65900AFP405
Alexa Fluor Plus 488	NB100-65900AFP488
Alexa Fluor Plus 555	NB100-65900AFP555
Alexa Fluor Plus 594	NB100-65900AFP594
Alexa Fluor Plus 647	NB100-65900AFP647
Alexa Fluor Plus 680	NB100-65900AFP680
Allophycocyanin	NB100-65900APC
Biotin	NB100-65900B
CoraFluor 1	NB100-65900CL1
DyLight 350	NB100-65900UV
DyLight 405	NB100-65900V
DyLight 488	NB100-65900G
DyLight 550	NB100-65900R
DyLight 594	NB100-65900DL594
DyLight 650	NB100-65900C
DyLight 680	NB100-65900FR
DyLight 755	NB100-65900IR
FITC	NB100-65900F-100ul
HRP	NB100-65900H
Janelia Fluor 525	NB100-65900JF525
Janelia Fluor 549	NB100-65900JF549
Janelia Fluor 646	NB100-65900JF646
Janelia Fluor 669	NB100-65900JF669
PE	NB100-65900PE
PerCP	NB100-65900PCP
mFluor Violet 450 SE	NB100-65900MFV450
mFluor Violet 500 SE	NB100-65900MFV500
mFluor Violet 610 SE	NB100-65900MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-65900-0.025mg

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

CD31/PECAM-1 Antibodies

CD31/PECAM-1 Antibody [Unconjugated]

CD31/PECAM-1 Antibody

CD31/PECAM-1 Antibody [Unconjugated]

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CD31/PECAM-1 Antibody (9G11) [Unconjugated]

CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free

CD31/PECAM-1 Antibody (MEC13.3) - BSA Free

CD31/PECAM-1 Antibody (MEC 7.46) - BSA Free

CD31/PECAM-1 Antibody - BSA Free

CD31/PECAM-1 Antibody (C31.3 + C31.7 + C31.10)

CD31/PECAM-1 Antibody (C31.3)

CD31/PECAM-1 Antibody (CO.3E1D4) - BSA Free