CD45 Antibody [Unconjugated]

CD45 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line (positive staining) and Neuro‑2A mouse neuroblastoma cell line (negative staining) using Goat Anti-Mouse CD45 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF114) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

CD45 was detected in immersion fixed paraffin-embedded sections of mouse liver using Goat Anti-Mouse CD45 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF114) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Mu
• Applications: WB, Flow, IHC, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CD45 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD45
  Gln24-Lys425
  Accession # NP_035340
• Specificity: Detects mouse CD45 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PTPRC
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD45 Antibody [Unconjugated]

• B220
• CD_antigen: CD45
• CD45 antigen
• CD45
• CD45R
• EC 3.1.3.48
• EC:3.1.3.48
• GP180
• LCA
• L-CA
• Leukocyte common antigen
• LY5
• protein tyrosine phosphatase, receptor type, c polypeptide
• PTPRC
• receptor-type tyrosine-protein phosphatase C
• T200 Glycoprotein
• T200 leukocyte common antigen
• T200

Background

Mouse CD45 (also known as Ly5 and leukocyte common antigen) is a 180‑220 kDa variably glycosylated member of the class 1 subtype of the protein tyrosine phosphatase family. It is synthesized as a 1291 amino acid (aa) precursor that contains a 23 aa signal sequence, a 541 aa extracellular domain (ECD), a 22 aa transmembrane segment, and a 705 aa cytoplasmic region. The ECD is coded for by exons 4‑16 of the CD45 gene. Alternate splicing of exon 4 (or A) (aa 30‑74), exon 5 (or B) (aa 75‑123) and exon 6 (or C) (aa 124‑169) define different lymphocyte populations and functional stages. Naïve T cells express exon 5 (CD45 RB), while activated T cells express neither exon 4, 5 or 6 (CD 45 RO). B cells express CD45 RABC, while resting NK cells express CD45 RA. Mouse CD45 ECD shares 60% and 44% aa sequence identity with rat and human full‑length CD45 ECD, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD45 Antibody (AF114)(30)

Publications using AF114

• A Xia, A Thai, Z Cao, X Chen, J Chen, B Bacacao, LA Bekale, V Schiel, PL Bollyky, PLS Maria Chronic suppurative otitis media causes macrophage-associated sensorineural hearing loss Journal of Neuroinflammation, 2022;19(1):224. 2022 [PMID: 36096817] (IHC, Mouse)
• K Xu, S Chen, L Xie, Y Qiu, XZ Liu, X Bai, Y Jin, XH Wang, Y Sun The protective effects of systemic dexamethasone on sensory epithelial damage and hearing loss in targeted Cx26-null mice Cell Death & Disease, 2022;13(6):545. 2022 [PMID: 35688810] (IHC, Mouse)
• G Huang, Z Cheng, A Hildebrand, C Wang, M Cimini, R Roy, AM Lucchese, C Benedict, V Mallaredy, A Magadum, D Joladarash, C Thej, C Gonzalez, M Trungcao, VNS Garikipati, JW Elrod, WJ Koch, R Kishore Diabetes impairs cardioprotective function of endothelial progenitor cell-derived extracellular vesicles via H3K9Ac inhibition Theranostics, 2022;12(9):4415-4430. 2022 [PMID: 35673580] (IHC, Mouse)
• A Glasow, I Patties, ND Priest, REJ Mitchel, G Hildebrand, K Manda Dose and Dose Rate-Dependent Effects of Low-Dose Irradiation on Inflammatory Parameters in ApoE-Deficient and Wild Type Mice Cells, 2021;10(11):. 2021 [PMID: 34831473] (ICC, Mouse)
• J Wang, A Rattner, J Nathans A transcriptome atlas of the mouse iris at single-cell resolution defines cell types and the genomic response to pupil dilation Elife, 2021;10(0):. 2021 [PMID: 34783308] (IHC, Mouse)
• SS Qin, BJ Han, A Williams, KM Jackson, R Jewell, AC Chacon, EM Lord, DC Linehan, M Kim, A Reuben, SA Gerber, PA Prieto Intertumoral Genetic Heterogeneity Generates Distinct Tumor Microenvironments in a Novel Murine Synchronous Melanoma Model Cancers, 2021;13(10):. 2021 [PMID: 34064795] (IHC, Mouse)
• J Filtjens, A Roger, L Quatrini, E Wieduwild, J Gouilly, G Hoeffel, R Rossignol, C Daher, G Debroas, S Henri, CM Jones, B Malissen, LK Mackay, A Moqrich, FR Carbone, S Ugolini Nociceptive sensory neurons promote CD8 T cell responses to HSV-1 infection Nature Communications, 2021;12(1):2936. 2021 [PMID: 34006861] (IHC, Mouse)
• RM Lasagni Vi, M Barbariga, P Fonteyne, F Bignami, P Rama, G Ferrari Modulating Ocular Surface Pain Through Neurokinin-1 Receptor Blockade Investigative Ophthalmology & Visual Science, 2021;62(3):26. 2021 [PMID: 33729475] (IHC, Mouse)
• AE Engler, AB Ysasi, RMF Pihl, C Villacorta, HM Heston, HMK Richardson, BR Thapa, NR Moniz, AC Belkina, SA Mazzilli, JR Rock Airway-Associated Macrophages in Homeostasis and Repair Cell Reports, 2020;33(13):108553. 2020 [PMID: 33378665] (IHC, Transgenic Mouse)
• MT Massengill, NF Ash, BM Young, CJ Ildefonso, AS Lewin Sectoral activation of glia in an inducible mouse model of autosomal dominant retinitis pigmentosa Sci Rep, 2020;10(1):16967. 2020 [PMID: 33046772]
• V Rai, MB Wood, H Feng, NM Schabla, S Tu, J Zuo The immune response after noise damage in the cochlea is characterized by a heterogeneous mix of adaptive and innate immune cells Scientific Reports, 2020;10(1):15167. 2020 [PMID: 32938973] (IHC, Mouse)
• SJ Jeong, MJ Cho, NY Ko, S Kim, IH Jung, JK Min, SH Lee, JG Park, GT Oh Deficiency of peroxiredoxin 2 exacerbates angiotensin II-induced abdominal aortic aneurysm Exp. Mol. Med., 2020;0(0):. 2020 [PMID: 32929220] (Western Blot, Mouse)
• A Shahapal, EB Cho, HJ Yong, I Jeong, H Kwak, JK Lee, W Kim, B Kim, HC Park, WS Lee, H Kim, JI Hwang, JY Seong FAM19A5 Expression During Embryogenesis and in the Adult Traumatic Brain of FAM19A5-LacZ Knock-in Mice Front Neurosci, 2019;13(0):917. 2019 [PMID: 31543758] (IHC, Mouse)
• S Schwager, S Renner, T Hemmerle, S Karaman, ST Proulx, R Fetz, AM Golding-Oc, P Probst, C Halin, D Neri, M Detmar Antibody-mediated delivery of VEGF-C potently reduces chronic skin inflammation JCI Insight, 2018;3(23):. 2018 [PMID: 30518687] (IHC, Mouse)
• C Maranto, V Udhane, DT Hoang, L Gu, V Alexeev, KM Malas, K Cardenas, JR Brody, U Rodeck, C Bergom, KA Iczkowski, K Jacobsohn, WA See, SM Schmitt, MT Nevalainen STAT5A/B BLOCKADE SENSITIZES PROSTATE CANCER TO RADIATION THROUGH INHIBITION OF RAD51 AND DNA REPAIR Clin. Cancer Res., 2018;0(0):. 2018 [PMID: 29483142] (IHC-Fr, Mouse)
• LJ Yockey, KA Jurado, N Arora, A Millet, T Rakib, KM Milano, AK Hastings, E Fikrig, Y Kong, TL Horvath, S Weatherbee, HJ Kliman, CB Coyne, A Iwasaki Type I interferons instigate fetal demise after Zika virus infection Sci Immunol, 2018;3(19):. 2018 [PMID: 29305462] (IHC, Mouse)
• J Liu, S Kumar, E Dolzhenko, GF Alvarado, J Guo, C Lu, Y Chen, M Li, MC Dessing, RK Parvez, PE Cippà, AM Krautzberg, G Saribekyan, AD Smith, AP McMahon Molecular characterization of the transition from acute to chronic kidney injury following ischemia/reperfusion JCI Insight, 2017;2(18):. 2017 [PMID: 28931758] (IHC-P, Mouse)
• MX Hernandez, S Jiang, TA Cole, SH Chu, MI Fonseca, MJ Fang, LA Hohsfield, MD Torres, KN Green, RA Wetsel, A Mortazavi, AJ Tenner Prevention of C5aR1 signaling delays microglial inflammatory polarization, favors clearance pathways and suppresses cognitive loss Mol Neurodegener, 2017;12(1):66. 2017 [PMID: 28923083] (IHC, Mouse)
• C Carmona-Fo, M Deforet, L Akkari, CB Thompson, JA Joyce, JB Xavier Metabolic origins of spatial organization in the tumor microenvironment Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):. 2017 [PMID: 28246332] (ICC, Human)
• Z Zuo, F Qi, J Yang, X Wang, Y Wu, Y Wen, Q Yuan, J Zou, K Guo, ZB Yao Immunization with Bacillus Calmette-Gu�rin (BCG) alleviates neuroinflammation and cognitive deficits in APP/PS1 mice via the recruitment of inflammation-resolving monocytes to the brain Neurobiol. Dis, 2017;101(0):27-39. 2017 [PMID: 28189498] (IHC, Mouse)
• Jong-Gil Park CREBH-FGF21 axis improves hepatic steatosis by suppressing adipose tissue lipolysis Sci Rep, 2016;6(0):27938. 2016 [PMID: 27301791] (IHC-P, Mouse)
• Mathias D, Mitchel R, Barclay M, Wyatt H, Bugden M, Priest N, Whitman S, Scholz M, Hildebrandt G, Kamprad M, Glasow A Low-dose irradiation affects expression of inflammatory markers in the heart of ApoE -/- mice. PLoS ONE, 2015;10(3):e0119661. 2015 [PMID: 25799423] (IHC, Mouse)
• Liakhovitskaia A, Rybtsov S, Smith T, Batsivari A, Rybtsova N, Rode C, de Bruijn M, Buchholz F, Gordon-Keylock S, Zhao S, Medvinsky A Runx1 is required for progression of CD41+ embryonic precursors into HSCs but not prior to this. Development, 2014;141(17):3319-23. 2014 [PMID: 25139854] (IHC, Mouse)
• Downstream Toll-like receptor signaling mediates adaptor-specific cytokine expression following focal cerebral ischemia. J Neuroinflammation, 2012;9(0):174. 2012 [PMID: 22799573] (IHC-Fr, Mouse)
• Gobbetti T, Cenac N, Motta JP, Rolland C, Martin L, Andrade-Gordon P, Steinhoff M, Barocelli E, Vergnolle N Serine protease inhibition reduces post-ischemic granulocyte recruitment in mouse intestine. Am. J. Pathol., 2012;180(1):141-52. 2012 [PMID: 22067907] (IHC-P, Mouse)
• Arora D, Stopp S, Bohmer SA, Schons J, Godfrey R, Masson K, Razumovskaya E, Ronnstrand L, Tanzer S, Bauer R, Bohmer FD, Muller JP Protein-tyrosine phosphatase DEP-1 controls receptor tyrosine kinase FLT3 signaling. J. Biol. Chem., 2011;286(13):10918-29. 2011 [PMID: 21262971] (Western Blot, Mouse)
• Kraman M, Bambrough P, Arnold J, Roberts E, Magiera L, Jones J, Gopinathan A, Tuveson D, Fearon D Suppression of antitumor immunity by stromal cells expressing fibroblast activation protein-alpha. Science, 0;330(6005):827-30. 0 [PMID: 21051638] (IHC, Mouse)
• Graham BB, Mentink-Kane MM, El-Haddad H Schistosomiasis-induced experimental pulmonary hypertension: role of interleukin-13 signaling. Am. J. Pathol., 2010;177(3):1549-61. 2010 [PMID: 20671265] (IHC-P, Mouse)
• Chou S, Lodish HF Fetal liver hepatic progenitors are supportive stromal cells for hematopoietic stem cells. Proc. Natl. Acad. Sci. U.S.A., 2010;107(17):7799-804. 2010 [PMID: 20385801] (ICC, Mouse)
• Tigges U, Hyer EG, Scharf J, Stallcup WB FGF2-dependent neovascularization of subcutaneous Matrigel plugs is initiated by bone marrow-derived pericytes and macrophages. Development, 2008;135(3):523-32. 2008 [PMID: 18171688] (ICC, Mouse)

Review for CD45 Antibody (AF114) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Anonymous - IP Other 01/05/2015

Summary

• Application: Immunoprecipitation
• Sample Tested: See PMID 22345665
• Species: Other

FAQs for CD45 Antibody (AF114). (Showing 1 - 4 of 4 FAQs).

1. We would like to order a CD45 antibody to stain immune cells that were isolated from a ligated sciatic nerve of mice with double immunofluorescence (using PFA-fixed tissue on slides). Which of the following rat monoclonal CD45 antibodies would you recommend: 30-F11, IBL-3/16 or 5C16?
   • I would recommend clone 30-F11. As clone IBL-3/16 has not yet been validated for IHC-P application, we would not be able to guarantee its workability on PFA-fixed tissue. Although both other clones i.e. 30-F11 and 5C16, would be good for your samples, CD45 30-F11 # NB100-77417 is a well known clone that offers more flexibility around protocol because of availability of its conjugated forms. It would be advantageous to use a conjugated primary as you are planning for double-immunostaining procedure.
2. For use in Western Blot with CD45 antibodies, what molecular weight of the band should I expect to see?
   • The theoretical molecular weight for most of our CD45 antibodies is 147 kDa based off the first isoform. Any variation on 147 is due to the immunogen being from a different species and the protein being a slightly different size. CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated.
3. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
   • If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product, allowing the tested application/species to fall under our 100% guarantee. Please check out our Innovator's Reward Program if you decide to test a primary antibody with a species or application that is not currently listed. Please note that the Innovator's Reward Program only applies to our primary antibodies.
4. Is CD45 a good target for use in Neurodegeneration studies?
   • Yes, here are the approved research areas we have listed for our CD45 products: Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Glia Markers, Hematopoietic Stem Cell Markers, Immunology, Innate Immunity, Mast Cell Markers, Mesenchymal Stem Cell Markers, Microglia Markers, Myeloid Cell Markers, Myeloid-derived Suppressor, Neurodegeneration, Neuroscience, Signal Transduction, Stem Cell Markers, Growth and Development.

Bioinformatics

• Gene Symbol: PTPRC
• Uniprot:
  • NP_035340()