CD45 Antibody [Unconjugated]


CD45 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line (positive staining) and Neuro‑2A mouse neuroblastoma cell line (negative staining) using Goat Anti-Mouse CD45 Antigen more
CD45 was detected in immersion fixed paraffin-embedded sections of mouse liver using Goat Anti-Mouse CD45 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF114) at 3 µg/mL for 1 hour at room temperature more

Product Details

Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, ICC/IF

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Catalog# & Formulation Size Price

CD45 Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant mouse CD45
Accession # NP_035340
Detects mouse CD45 in direct ELISAs and Western blots.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 4
AF114 in the following applications:

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AF114 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD45 Antibody [Unconjugated]

  • B220
  • CD_antigen: CD45
  • CD45 antigen
  • CD45
  • CD45R
  • EC
  • EC:
  • GP180
  • LCA
  • L-CA
  • Leukocyte common antigen
  • LY5
  • protein tyrosine phosphatase, receptor type, c polypeptide
  • receptor-type tyrosine-protein phosphatase C
  • T200 Glycoprotein
  • T200 leukocyte common antigen
  • T200


Mouse CD45 (also known as Ly5 and leukocyte common antigen) is a 180‑220 kDa variably glycosylated member of the class 1 subtype of the protein tyrosine phosphatase family. It is synthesized as a 1291 amino acid (aa) precursor that contains a 23 aa signal sequence, a 541 aa extracellular domain (ECD), a 22 aa transmembrane segment, and a 705 aa cytoplasmic region. The ECD is coded for by exons 4‑16 of the CD45 gene. Alternate splicing of exon 4 (or A) (aa 30‑74), exon 5 (or B) (aa 75‑123) and exon 6 (or C) (aa 124‑169) define different lymphocyte populations and functional stages. Naïve T cells express exon 5 (CD45 RB), while activated T cells express neither exon 4, 5 or 6 (CD 45 RO). B cells express CD45 RABC, while resting NK cells express CD45 RA. Mouse CD45 ECD shares 60% and 44% aa sequence identity with rat and human full‑length CD45 ECD, respectively.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD45 Antibody (AF114)(30)

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 5 applications: ICC, IHC, IHC-Fr, IHC-P, Western Blot.

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Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using AF114 Applications Species
A Xia, A Thai, Z Cao, X Chen, J Chen, B Bacacao, LA Bekale, V Schiel, PL Bollyky, PLS Maria Chronic suppurative otitis media causes macrophage-associated sensorineural hearing loss Journal of Neuroinflammation, 2022;19(1):224. 2022 [PMID: 36096817] (IHC, Mouse) IHC Mouse
K Xu, S Chen, L Xie, Y Qiu, XZ Liu, X Bai, Y Jin, XH Wang, Y Sun The protective effects of systemic dexamethasone on sensory epithelial damage and hearing loss in targeted Cx26-null mice Cell Death & Disease, 2022;13(6):545. 2022 [PMID: 35688810] (IHC, Mouse) IHC Mouse
G Huang, Z Cheng, A Hildebrand, C Wang, M Cimini, R Roy, AM Lucchese, C Benedict, V Mallaredy, A Magadum, D Joladarash, C Thej, C Gonzalez, M Trungcao, VNS Garikipati, JW Elrod, WJ Koch, R Kishore Diabetes impairs cardioprotective function of endothelial progenitor cell-derived extracellular vesicles via H3K9Ac inhibition Theranostics, 2022;12(9):4415-4430. 2022 [PMID: 35673580] (IHC, Mouse) IHC Mouse
A Glasow, I Patties, ND Priest, REJ Mitchel, G Hildebrand, K Manda Dose and Dose Rate-Dependent Effects of Low-Dose Irradiation on Inflammatory Parameters in ApoE-Deficient and Wild Type Mice Cells, 2021;10(11):. 2021 [PMID: 34831473] (ICC, Mouse) ICC Mouse
J Wang, A Rattner, J Nathans A transcriptome atlas of the mouse iris at single-cell resolution defines cell types and the genomic response to pupil dilation Elife, 2021;10(0):. 2021 [PMID: 34783308] (IHC, Mouse) IHC Mouse
SS Qin, BJ Han, A Williams, KM Jackson, R Jewell, AC Chacon, EM Lord, DC Linehan, M Kim, A Reuben, SA Gerber, PA Prieto Intertumoral Genetic Heterogeneity Generates Distinct Tumor Microenvironments in a Novel Murine Synchronous Melanoma Model Cancers, 2021;13(10):. 2021 [PMID: 34064795] (IHC, Mouse) IHC Mouse
J Filtjens, A Roger, L Quatrini, E Wieduwild, J Gouilly, G Hoeffel, R Rossignol, C Daher, G Debroas, S Henri, CM Jones, B Malissen, LK Mackay, A Moqrich, FR Carbone, S Ugolini Nociceptive sensory neurons promote CD8 T cell responses to HSV-1 infection Nature Communications, 2021;12(1):2936. 2021 [PMID: 34006861] (IHC, Mouse) IHC Mouse
RM Lasagni Vi, M Barbariga, P Fonteyne, F Bignami, P Rama, G Ferrari Modulating Ocular Surface Pain Through Neurokinin-1 Receptor Blockade Investigative Ophthalmology & Visual Science, 2021;62(3):26. 2021 [PMID: 33729475] (IHC, Mouse) IHC Mouse
AE Engler, AB Ysasi, RMF Pihl, C Villacorta, HM Heston, HMK Richardson, BR Thapa, NR Moniz, AC Belkina, SA Mazzilli, JR Rock Airway-Associated Macrophages in Homeostasis and Repair Cell Reports, 2020;33(13):108553. 2020 [PMID: 33378665] (IHC, Transgenic Mouse) IHC Transgenic Mouse
MT Massengill, NF Ash, BM Young, CJ Ildefonso, AS Lewin Sectoral activation of glia in an inducible mouse model of autosomal dominant retinitis pigmentosa Sci Rep, 2020;10(1):16967. 2020 [PMID: 33046772]
Show All 30 Publications.

Review for CD45 Antibody (AF114) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using AF114:
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Images Ratings Applications Species Date Details
reviewed by:
Anonymous -
IP Other 01/05/2015


Sample TestedSee PMID 22345665

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CD45 Antibody (AF114). (Showing 1 - 4 of 4 FAQs).

  1. We would like to order a CD45 antibody to stain immune cells that were isolated from a ligated sciatic nerve of mice with double immunofluorescence (using PFA-fixed tissue on slides). Which of the following rat monoclonal CD45 antibodies would you recommend: 30-F11, IBL-3/16 or 5C16?
    • I would recommend clone 30-F11. As clone IBL-3/16 has not yet been validated for IHC-P application, we would not be able to guarantee its workability on PFA-fixed tissue. Although both other clones i.e. 30-F11 and 5C16, would be good for your samples, CD45 30-F11 # NB100-77417 is a well known clone that offers more flexibility around protocol because of availability of its conjugated forms. It would be advantageous to use a conjugated primary as you are planning for double-immunostaining procedure.
  2. For use in Western Blot with CD45 antibodies, what molecular weight of the band should I expect to see?
    • The theoretical molecular weight for most of our CD45 antibodies is 147 kDa based off the first isoform. Any variation on 147 is due to the immunogen being from a different species and the protein being a slightly different size. CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated.
  3. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
    • If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product, allowing the tested application/species to fall under our 100% guarantee. Please check out our Innovator's Reward Program if you decide to test a primary antibody with a species or application that is not currently listed. Please note that the Innovator's Reward Program only applies to our primary antibodies.
  4. Is CD45 a good target for use in Neurodegeneration studies?
    • Yes, here are the approved research areas we have listed for our CD45 products: Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Glia Markers, Hematopoietic Stem Cell Markers, Immunology, Innate Immunity, Mast Cell Markers, Mesenchymal Stem Cell Markers, Microglia Markers, Myeloid Cell Markers, Myeloid-derived Suppressor, Neurodegeneration, Neuroscience, Signal Transduction, Stem Cell Markers, Growth and Development.

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You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

CD45 - Much more than just a housekeeping protein
CD45, also known as T200 or the Leukocyte Common Antigen (LCA), is encoded by the Protein Tyrosine Phosphatase Receptor type C (PTPRC) gene. The protein is expressed exclusively on cells of the haematopoietic system, and is one of the most abundant le...  Read full blog post.

CD45 Isoforms: Hematopoietic Differentiation, Cancer and Alzheimer's
CD45, also known as protein tyrosine phosphatase, receptor type, C (PTPRC), was originally known as common leukocyte antigen and is a signal transducer involved in many physiological processes such as growth and differentiation, cancer transformation,...  Read full blog post.

Vimentin Antibodies in Rheumatoid Arthritis & Cataracts Research
Vimentin is a 57kDa type III intermediate filament (IF) protein that is the major cytoskeletal component of mesenchymal cells and the first to be expressed during cell differentiation. It plays a significant role in supporting and anchoring the positi...  Read full blog post.

Antibodies In The Differential Diagnosis Of Undifferentiated Tumors
Immunohistochemical testing using antibody panels has a valuable role in cancer diagnosis. Some tumors, especially more malignant ones, tend to lose the appearance of the tissue of origin. However, knowing the tissue of origin assists the physician in...  Read full blog post.

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Recent Reviews


Anonymous -
Application: IP
Species: Other


Gene Symbol PTPRC