Vimentin Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Vimentin Antibody [NB300-223] - View of mixed neuron/glial cultures stained with NB300-223 (green) and rabbit antibody to GFAP antibody NB300-141(red). Vimentin is expressed ...read more
Western Blot: Vimentin Antibody [NB300-223] - Western blot of crude extract of human embryonic kidney Hek293 cells stained with NB300-223, showing a single strong clean band at approx. 50kDa.

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated

Order Details

Vimentin Antibody Summary

Immunogen
Recombinant human Vimentin purified from E. coli. [Swiss-Prot# P08670]
Marker
Mesenchymal Cells Marker
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
VIM
Purity
IgY purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:5000
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:150
Application Notes
This Vimentin antibody is useful for Immunohistochemistry paraffin embedded sections, Western blot and Immunocytochemistry/Immunofluorescence. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 25626686) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB300-223 in the following applications:

Publications
Read Publications using
NB300-223 in the following applications:

Reactivity Notes

Human, mouse, and rat. Canine reactivity reported in scientific literature (PMID: 26616495)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Antibody is supplied as an aliquot of concentrated IgY prep.
Preservative
5mM Sodium Azide
Purity
IgY purified

Alternate Names for Vimentin Antibody

  • FLJ36605
  • VIM
  • Vimentin

Background

Vimentin (VIM) is a widely expressed/highly conserved member of type III intermediate filaments (IF) family proteins found in various non-epithelial cells, especially in mesenchymal cells. It exists as homopolymer assembled from elementary dimers and interacts with HCV core protein, LGSN, SYNM, PLEC, SLC6A4, STK33, LARP6 and RAB8B, and its interaction with LARP6 plays a key role in the stabilization of type I collagen mRNAs, CO1A1/CO1A2. VIM's dynamic structural changes and spatial re-organization in response to extracellular stimuli facilitate coordination of various signal transduction pathways. Filament disassembly during cell division is promoted by VIM phosphorylation at Ser-55 as well as by nestin. VIM is phosphorylated by STK33, CDK5 and PKN1; and PKN1 mediated phosphorylation inhibits filaments formation, whereas, CDK5 executes its phosphorylation at Ser-56 during neutrophil secretion in cytoplasm. VIM expression is predominant during embryonic development, while in adults, its expression is limited to connective tissue mesenchymal cells, CNS and muscles. VIM has emerged as canonical marker of EMT (epithelial to mesenchymal transition- a process that renders the epithelial cells to dramatically alter their shape and acquire increased motility) which is characterized by the expression of VIM IFs in epithelial cells that normally express only keratin IFs and enhanced VIM expression has been reported in various cancers where it plays as a driving factor for metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Vimentin Antibody (NB300-223)(18)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(7)
IHC
(5)
IHC-Fr
(4)
IHC-P
(1)
WB
(3)
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Filter By Species
Human
(6)
Mouse
(10)
Rat
(1)
Canine
(1)
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Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NB300-223 Applications Species
Behrens J, Solga R, Ziemann A et al. Coronin 1C-free primary mouse fibroblasts exhibit robust rearrangements in the orientation of actin filaments, microtubules and intermediate filaments. Eur. J. Cell Biol. May 4 2016 [PMID: 27178841] (ICC/IF, Mouse) ICC/IF Mouse
Nakamura R, Koshiba-Takeuchi K, Tsuchiya M et al. Expression analysis of Baf60c during heart regeneration in axolotls and neonatal mice. Dev. Growth Differ. May 1 2016 [PMID: 27125315] (ICC/IF, Mouse) ICC/IF Mouse
Smith LR, Hammers DW, Sweeney HL, Barton ER. Increased collagen cross-linking is a signature of dystrophin-deficient muscle. Muscle Nerve. 2015 Nov 30 [PMID: 26616495] (IHC-Fr, Human, Mouse, Canine)

Details:
Vimentin antibody used for immunofluorescence on cryosections of mouse, canine and human quadriceps muscle at a dilution of 1:800 (Figure 3).
IHC-Fr Human, Mouse, Canine
Ge X, Pettan-Brewer C, Morton J et al. Mitochondrial catalase suppresses naturally occurring lung cancer in old mice. Pathobiol Aging Age Relat Dis 2015 Sep 24 [PMID: 26400209] (IHC, Mouse) IHC Mouse
Wong JK, Chen L, Huang Y et al. Attenuation of Cerebral Ischemic Injury in Smad1 Deficient Mice. PLoS ONE 2015 Aug 31 [PMID: 26317208] (IHC-Fr, Mouse) IHC-Fr Mouse
Morissette Martin P, Maux A, Laterreur V et al. Enhancing repair of full-thickness excisional wounds in a murine model: Impact of tissue-engineered biological dressings featuring human differentiated adipocytes. Acta Biomater. 2015 Apr 29 [PMID: 25934321] (IHC-Fr, Mouse) IHC-Fr Mouse
Pocsai K, Kalman M. Glial and perivascular structures in the subfornical organ: distinguishing the shell and core. J Histochem Cytochem 2015 May [PMID: 25673286]
Marcuzzo S, Bonanno S, Kapetis D et al. Up-regulation of neural and cell cycle-related microRNAs in brain of amyotrophic lateral sclerosis mice at late disease stage Mol Brain. 2015 Jan 28 [PMID: 25626686] (IHC-Fr, Mouse) IHC-Fr Mouse
Phamduong E, Rathore MK, Crews NR et al. Acute and Chronic Mu Opioids Differentially Regulate Thrombospondins 1 and 2 Isoforms in Astrocytes. ACS Chem Neurosci 2013 Dec 5 [PMID: 24304333] (ICC/IF, Rat) ICC/IF Rat
Murray ME, Mendez MG, Janmey PA et al. Substrate Stiffness Regulates Solubility of Cellular Vimentin. Mol Biol Cell. 2013 Oct 30 [PMID: 24173714] (ICC/IF, WB, Human) ICC/IF, WB Human
Show All 18 Publications.

Review for Vimentin Antibody (NB300-223) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: ICC.

Reviews using NB300-223:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Vimentin NB300-223
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reviewed by:
Anonymous
ICC Mouse 06/15/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedNIH-3T3 mouse embryonic fibroblast cell line
SpeciesMouse
Lot91412

Product General Protocols

View specific protocols for Vimentin Antibody (NB300-223): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Vimentin Antibody (NB300-223). (Showing 1 - 2 of 2 FAQs).

  1. Could you please tell me if this antibody is IgG or IgY? Also, have any of your secondary Cy5 antibodies been tested with it?
    • NB300-223 is a polyclonal antibody, and as such the isotype has not been determined. I recommend using an anti-chicken IgG secondary. We have not tested any Cy5-conjugated secondaries with this primary, but we do guarantee all of our products 100% for the species and applications listed on the data sheets.
  2. We have a customer who is looking for a chicken antibody for co-IF staining of mouse heart tissues. I found NB300-223. May I ask what species was used for the IF staining seen in the image on the data sheet? Do you know if the antibody has been specifically tested with mouse tissues in IF or IHC?
    • The image on our datasheet was obtained from rat neurons. This antibody doesn't look like it has been specifically tested on mouse tissues, but the rat and mouse proteins are 99.5% identical, and the human and mouse proteins are 98% identical so I see no reason why this antibody wouldn't work on mouse tissue.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Vimentin Antibody (NB300-223)

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Blogs on Vimentin.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

The use of actin as a loading control in research on fruiting-body development and vegetative growth in Sordaria macrospora research
Sordaria macrospora is a filamentous fungus that serves as very useful system for scientific research due to a short life cycle and easy manipulation.  Just like any other model organism, it is important to have an effective loading control to va...  Read full blog post.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

Vimentin: Regulating EMT and Cancer
Vimentin, a member of the intermediate filament (IF) family, is a protein responsible for maintaining cellular integrity and reducing damage caused by stress. The vimentin protein is ubiquitously expressed in normal mesenchymal cells, and recent resea...  Read full blog post.

Vimentin in Wound Healing
Vimentin is a fundamental 10 nm type III intermediate filament (IF) protein found in many mesenchymal and epithelia tissues, tissue culture cells, and developing neuronal and astrocytic precursor cells of the central nervous system. It frequently co-p...  Read full blog post.

Vimentin as a Marker for Epithelial-to-Mesenchymal Transition
Epithelial-to-mesenchymal transition (EMT) is a critical event in the induction of cell motility and increased survival both under physiological situations like wound healing or development, as well as in malignant cells undergoing invasion and metast...  Read full blog post.

Vimentin Antibodies in Rheumatoid Arthritis & Cataracts Research
Vimentin is a 57kDa type III intermediate filament (IF) protein that is the major cytoskeletal component of mesenchymal cells and the first to be expressed during cell differentiation. It plays a significant role in supporting and anchoring the positi...  Read full blog post.

ZMPSTE24 Mutations, Lamin A Processing & Laminopathies
ZMPSTE24 (FACE-1, CAAX prenyl protease 1 homolog) is a membrane associated zinc metalloprotease of the peptidase M48A family. It's catalytic activity can be defined as the peptide bond hydrolyzed in the sequence -C-|-A-A-X in which C is an S-isoprenyl...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
06/15/2017
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol VIM
Entrez
Uniprot