Vimentin Antibody

Images

 
Western Blot: Vimentin Antibody [NB300-223] - Analysis of tissue and cell lysates. Antibody at 1:5000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 ...read more
Immunohistochemistry: Vimentin Antibody [NB300-223] - Attenuated reactive astrocytosis after stroke in Smad1 cKO mice. Enlarged IHC images of boxed areas at the cortical peri-infarct area with the indicated reactive ...read more
Immunocytochemistry/ Immunofluorescence: Vimentin Antibody [NB300-223] - Human A7 cells. Cells were fixed with 10% formalin and permeabilized with 0.1% TritonX-100. After blocking, cells were incubated with primary ...read more
Immunohistochemistry: Vimentin Antibody [NB300-223] - Increased numbers of differentiating NSPCs in SVZ of G93A-SOD1 brain. Confocal microscopy images of dorsal and ventral regions of SVZ in G93A-SOD1 and Wt-SOD1 brain ...read more
Immunocytochemistry/ Immunofluorescence: Vimentin Antibody [NB300-223] - Comparative analysis of epithelial and mesenchymal markers expressions. Fluorescence immunocytochemistry staining for E-cadherin (green), ...read more
Immunocytochemistry/ Immunofluorescence: Vimentin Antibody [NB300-223] - Analysis of HeLa cell culture. Antibody at 1:10,000 in green, and costained with mouse mAb to actin, dilution 1:500 in red. The blue is DAPI ...read more
Immunohistochemistry-Frozen: Vimentin Antibody [NB300-223] - 10 um Cryosections from PFA fixed murine brain acute slices stained with Vimentin antibody (1:600) and Alexa Fluor 488 anti chicken IgG. Antibody showed ...read more
Immunohistochemistry-Frozen: Vimentin Antibody [NB300-223] - Infarcted mouse heart frozen sections stained with vimentin antibody at 1:200. Secondary antibody at 1:500. Fixation with 4% PFA. IHC-Fr image submitted by a ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, EqSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated

Order Details

Vimentin Antibody Summary

Immunogen
Full length recombinant human Vimentin Antibody expressed in and purified from E. coli. [Swiss-Prot# P08670]
Marker
Mesenchymal Cells Marker
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
VIM
Purity
IgY purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:5000
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:150
Application Notes
Use in IHC-P and IHC-Fr reported in scientific literature (PMID: 23752194 and 25626686).
Theoretical MW
53.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Vimentin Knockout HeLa Cell Lysate
Reviewed Applications
Read 6 Reviews rated 5
using
NB300-223 in the following applications:

Publications
Read Publications using
NB300-223 in the following applications:

  • EM
    1 publication
  • 25 publications
  • IHC
    7 publications
  • 5 publications
  • 2 publications
  • KD
    1 publication
  • WB
    4 publications

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 26616495).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Supplied as concentrated IgY in PBS
Preservative
0.02% Sodium Azide
Purity
IgY purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for Vimentin Antibody

  • epididymis secretory sperm binding protein
  • FLJ36605
  • VIM
  • Vimentin

Background

Vimentin is a type III intermediate filament (IF) protein, encoded by the VIM gene, that plays an essential role in cytoskeletal function and is largely expressed by mesenchymal cell such as endothelial cells, fibroblasts, and immune cells (1). Human vimentin monomer protein is 466 amino acids (aa) in length with a theoretical molecular weight of ~54 kDa (2). Vimentin protein contains a central rod domain composed of four alpha-helices which is flanked by an N-terminal head domain and C-terminal tail domain (1,3). Vimentin monomers dimerize to form more complex structures resulting in the assembly of eight tetramers, referred to as unit-length filaments, which then assemble into full-length intermediate filaments that are 10-12 nm wide (1,3,4).

Activated macrophages have been shown to secrete phosphorylated vimentin which can be stimulated by a variety of pathophysiological factors including oxidized low-density lipoproteins and TNF-alpha or inhibited by IL-10 (1). The vimentin protein is often expressed at the cell surface playing a role in cell-cell interactions, tissue damage and repair, immune response, and pathogen recognition (1). Vimentin functions in many cytoskeletal processes including cell migration, which is highlighted by its upregulation during epithelial-to-mesenchymal transition (EMT) (4,5). Vimentin is a commonly used marker for EMT and is expressed by many tumor types (5). For example, high metastasis of oral squamous cell carcinomas also showed high vimentin positive expression in immunohistochemical staining analysis (5). A number of vimentin targeting compounds are in cancer-related clinical trials, however, given the multifunctional role of vimentin, the effect of inhibition on non-malignant cells needs to be thoroughly examined (5).

References

1. Ramos, I., Stamatakis, K., Oeste, C. L., & Perez-Sala, D. (2020). Vimentin as a Multifaceted Player and Potential Therapeutic Target in Viral Infections. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms21134675

2. Uniprot (P08670)

3. Morrow, C. S., & Moore, D. L. (2020). Vimentin's side gig: Regulating cellular proteostasis in mammalian systems. Cytoskeleton (Hoboken, N.J.). https://doi.org/10.1002/cm.21645

4. van Bodegraven, E. J., & Etienne-Manneville, S. (2020). Intermediate filaments against actomyosin: the david and goliath of cell migration. Current Opinion in Cell Biology. https://doi.org/10.1016/j.ceb.2020.05.006

5. Strouhalova, K., Prechova, M., Gandalovicova, A., Brabek, J., Gregor, M., & Rosel, D. (2020). Vimentin Intermediate Filaments as Potential Target for Cancer Treatment. Cancers. https://doi.org/10.3390/cancers12010184

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Vimentin Antibody (NB300-223)(47)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 7 applications: EM, ICC/IF, IHC, IHC-Fr, IHC-P, KD, WB.


Filter By Application
EM
(1)
ICC/IF
(25)
IHC
(7)
IHC-Fr
(5)
IHC-P
(2)
KD
(1)
WB
(4)
All Applications
Filter By Species
Human
(17)
Mouse
(19)
Rat
(2)
Canine
(1)
All Species
Showing Publications 1 - 10 of 47. Show All 47 Publications.
Publications using NB300-223 Applications Species
Suprewicz Ł, Swoger M, Gupta S, et al. Vimentin binds to SARS-CoV-2 spike protein and antibodies targeting extracellular vimentin block in vitro uptake of SARS-CoV-2 virus-like particles bioRxiv : the preprint server for biology Jan 8 2021 [PMID: 33442680] (ICC/IF, Human) ICC/IF Human
Ke Y, Fan X, Hao R, et al. Human embryonic stem cell-derived extracellular vesicles alleviate retinal degeneration by upregulating Oct4 to promote retinal Müller cell retrodifferentiation via HSP90 Stem cell research & therapy Jan 7 2021 [PMID: 33413616] (ICC/IF) ICC/IF
Li J, Zhang L, Zheng Y et al. BAD inactivation exacerbates rheumatoid arthritis pathology by promoting survival of sublining macrophages eLife Dec 3 2020 [PMID: 33270017] (ICC/IF) ICC/IF
Ding I, Ostrowska-Podhorodecka Z, Lee W et al. Cooperative roles of PAK1 and filamin A in regulation of vimentin assembly and cell extension formation Biochim Biophys Acta Mol Cell Res May 7 2020 [PMID: 32389644]
Zhou X, Wahane S, Friedl Ms et Al. Microglia and macrophages promote corralling, wound compaction and recovery after spinal cord injury via Plexin-B2 Nat. Neurosci. Mar 1 2020 [PMID: 32112058] (IHC-P, Mouse) IHC-P Mouse
Patteson AE, Pogoda K, Byfield FJ et al. Loss of Vimentin Enhances Cell Motility through Small Confining Spaces Small Nov 13 2019 [PMID: 31721440]
Ardalani H, Sengupta S, Harms V et al. 3-D Culture and Endothelial Cells Improve Maturity of Human Pluripotent Stem Cell-Derived Hepatocytes Acta Biomater Jul 27 2019 [PMID: 31362140] (ICC/IF, Human) ICC/IF Human
Patteson A, Pogoda K, Byfield F et al. Loss of vimentin intermediate filaments decreases peri-nuclear stiffness and enhances cell motility through confined spaces. arXiv preprint arXiv Jul 17 2018
Baker MJ, Cooke M, Kreider-Letterman G et al. Evaluation of active Rac1 levels in cancer cells: a case of misleading conclusions from immunofluorescence analysis J. Biol. Chem. Aug 14 2020 [PMID: 32817335] (ICC/IF, Human) ICC/IF Human
Patteson A E, Vahabikashi A et al. Vimentin protects cells against nuclear rupture and DNA damage during migration. J Cell Biol Feb 12 2019 [PMID: 31676718] (ICC/IF, Mouse) ICC/IF Mouse
Show All 47 Publications.

Reviews for Vimentin Antibody (NB300-223) (6) 56

Average Rating: 5
(Based on 6 reviews)
We have 6 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using NB300-223:
Filter by Applications
IHC-Fr
(3)
ICC
(3)
All Applications
Filter by Species
Human
(1)
Mouse
(3)
Rat
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Vimentin NB300-223
Enlarge
5
reviewed by:
Anonymous
IHC-Fr Mouse 01/30/2020
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse heart frozen sections,mouse frozen heart
SpeciesMouse
Lot6814

Comments

CommentsMouse heart after myocardial infarction
Frozen section
4% PFA fixation
Primary antibody concentration: 1:200
Secondary antibody concentration: 1:500
Immunocytochemistry Vimentin NB300-223
Enlarge
5
reviewed by:
Anonymous
ICC Human 04/22/2019
View

Summary

ApplicationImmunocytochemistry
Sample TestedA7 cells
SpeciesHuman
Lot6816-4

Comments

CommentsCells were fixed with 10% formalin and permeabilized with 0.1% TritonX-100. After blocking, cells were incubated with primary antibody (1:600) at 4℃ O/N followed with Alexa Fluor-488 labeled secondary antibody and counterstained with DAPI and Phalloidin for nuclei and F-actin respectively. Results were imaged with Leica TCS SP5 confocal microscope.
Immunohistochemistry-Frozen Vimentin NB300-223
Enlarge
5
reviewed by:
Anonymous
IHC-Fr Mouse 03/11/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Tested Mouse brain
SpeciesMouse
Lot6816-4
Immunohistochemistry-Frozen Vimentin NB300-223
Enlarge
5
reviewed by:
Anonymous
IHC-Fr Rat 06/15/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedRat Kidney Sections (Fresh Frozen)
SpeciesRat
Lot91412
Immunocytochemistry Vimentin NB300-223
Enlarge
5
reviewed by:
Anonymous
ICC Rat 06/15/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedNRK52E
SpeciesRat
Lot91412
Immunocytochemistry Vimentin NB300-223
Enlarge
5
reviewed by:
Anonymous
ICC Mouse 06/15/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedNIH-3T3 mouse embryonic fibroblast cell line
SpeciesMouse
Lot91412

Product General Protocols

View specific protocols for Vimentin Antibody (NB300-223): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Vimentin Antibody (NB300-223). (Showing 1 - 2 of 2 FAQs).

  1. Could you please tell me if this antibody is IgG or IgY? Also, have any of your secondary Cy5 antibodies been tested with it?
    • NB300-223 is a polyclonal antibody, and as such the isotype has not been determined. I recommend using an anti-chicken IgG secondary. We have not tested any Cy5-conjugated secondaries with this primary, but we do guarantee all of our products 100% for the species and applications listed on the data sheets.
  2. We have a customer who is looking for a chicken antibody for co-IF staining of mouse heart tissues. I found NB300-223. May I ask what species was used for the IF staining seen in the image on the data sheet? Do you know if the antibody has been specifically tested with mouse tissues in IF or IHC?
    • The image on our datasheet was obtained from rat neurons. This antibody doesn't look like it has been specifically tested on mouse tissues, but the rat and mouse proteins are 99.5% identical, and the human and mouse proteins are 98% identical so I see no reason why this antibody wouldn't work on mouse tissue.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Vimentin. Showing 1-10 of 13 blog posts - Show all blog posts.


  Read full blog post.

Antibody treatment can generate microglia-like cells from bone marrow
By Jennifer Sokolowski, MD, PhD.Microglia play important roles in the brain in both homeostatic and pathological conditions, acting to clear debris and dying cells. There is evidence to suggest that microglial dys...  Read full blog post.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

The use of actin as a loading control in research on fruiting-body development and vegetative growth in Sordaria macrospora research
Sordaria macrospora is a filamentous fungus that serves as very useful system for scientific research due to a short life cycle and easy manipulation.  Just like any other model organism, it is important to have an effective loading control to va...  Read full blog post.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

Vimentin: Regulating EMT and Cancer
Vimentin, a member of the intermediate filament (IF) family, is a protein responsible for maintaining cellular integrity and reducing damage caused by stress. The vimentin protein is ubiquitously expressed in normal mesenchymal cells, and recent resea...  Read full blog post.

Vimentin in Wound Healing
Vimentin is a fundamental 10 nm type III intermediate filament (IF) protein found in many mesenchymal and epithelia tissues, tissue culture cells, and developing neuronal and astrocytic precursor cells of the central nervous system. It frequently co-p...  Read full blog post.

Showing 1-10 of 13 blog posts - Show all blog posts.

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Recent Reviews

5
5
6
4
0
3
0
2
0
1
0

Anonymous
01/30/2020
Application: IHC-Fr
Species: Mouse

Anonymous
04/22/2019
Application: ICC
Species: Human

Anonymous
03/11/2019
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol VIM
Entrez
Uniprot