Western Blot: Vimentin Antibody [NB300-223] - Western blot of crude extract of human embryonic kidney Hek293 cells stained with NB300-223, showing a single strong clean band at approx. 50kDa.
Immunocytochemistry/ Immunofluorescence: Vimentin Antibody [NB300-223] - View of mixed neuron/glial cultures stained with NB300-223 (green) and rabbit antibody to GFAP antibody NB300-141(red). Vimentin is expressed ...read more
Hu, Mu, Rt, CaSpecies Glossary
WB, ICC/IF, IHC, IHC-Fr, IHC-P
Please see the vial label for concentration. If unlisted please contact technical services.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
5mM Sodium Azide
Ammonium sulfate precipitation
Western Blot 1:10000-1:20000
This Vimentin antibody is useful for Immunohistochemistry paraffin embedded sections, Western blot and Immunocytochemistry/Immunofluorescence. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 25626686) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Human, mouse, and rat. Canine reactivity reported in scientific literature (PMID: 26616495)
Alternate Names for Vimentin Antibody
Vimentin (VIM) is a widely expressed/highly conserved member of type III intermediate filaments (IF) family proteins found in various non-epithelial cells, especially in mesenchymal cells. It exists as homopolymer assembled from elementary dimers and interacts with HCV core protein, LGSN, SYNM, PLEC, SLC6A4, STK33, LARP6 and RAB8B, and its interaction with LARP6 plays a key role in the stabilization of type I collagen mRNAs, CO1A1/CO1A2. VIM's dynamic structural changes and spatial re-organization in response to extracellular stimuli facilitate coordination of various signal transduction pathways. Filament disassembly during cell division is promoted by VIM phosphorylation at Ser-55 as well as by nestin. VIM is phosphorylated by STK33, CDK5 and PKN1; and PKN1 mediated phosphorylation inhibits filaments formation, whereas, CDK5 executes its phosphorylation at Ser-56 during neutrophil secretion in cytoplasm. VIM expression is predominant during embryonic development, while in adults, its expression is limited to connective tissue mesenchymal cells, CNS and muscles. VIM has emerged as canonical marker of EMT (epithelial to mesenchymal transition- a process that renders the epithelial cells to dramatically alter their shape and acquire increased motility) which is characterized by the expression of VIM IFs in epithelial cells that normally express only keratin IFs and enhanced VIM expression has been reported in various cancers where it plays as a driving factor for metastasis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Vimentin Antibody (NB300-223). (Showing 1 - 2 of 2 FAQs).
Could you please tell me if this antibody is IgG or IgY? Also, have any of your secondary Cy5 antibodies been tested with it?
NB300-223 is a polyclonal antibody, and as such the isotype has not been determined. I recommend using an anti-chicken IgG secondary. We have not tested any Cy5-conjugated secondaries with this primary, but we do guarantee all of our products 100% for the species and applications listed on the data sheets.
We have a customer who is looking for a chicken antibody for co-IF staining of mouse heart tissues. I found NB300-223. May I ask what species was used for the IF staining seen in the image on the data sheet? Do you know if the antibody has been specifically tested with mouse tissues in IF or IHC?
The image on our datasheet was obtained from rat neurons. This antibody doesn't look like it has been specifically tested on mouse tissues, but the rat and mouse proteins are 99.5% identical, and the human and mouse proteins are 98% identical so I see no reason why this antibody wouldn't work on mouse tissue.
Vimentin: Regulating EMT and Cancer Vimentin, a member of the intermediate filament (IF) family, is a protein responsible for maintaining cellular integrity and reducing damage caused by stress. The vimentin protein is ubiquitously expressed in normal mesenchymal cells, and recent resea... Read full blog post.
Vimentin in Wound Healing Vimentin is a fundamental 10 nm type III intermediate filament (IF) protein found in many mesenchymal and epithelia tissues, tissue culture cells, and developing neuronal and astrocytic precursor cells of the central nervous system. It frequently co-p... Read full blog post.