beta-Actin Antibody


Western Blot: beta-Actin Antibody [NB600-503] - Western blot analysis of Jurkat, COS, and CHO cell lysate using beta actin antibody at 1:100.
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody [NB600-503] - IF Confocal analysis of HeLa cells using Beta Actin antibody (NB600-503, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as more
Immunohistochemistry-Paraffin: beta-Actin Antibody [NB600-503] - Analysis of Beta Actin in mouse epidermis using DAB with hematoxylin counterstain.
Flow Cytometry: beta-Actin Antibody [NB600-503] - analysis of HeLa cells using mouse Monoclonal beta-Actin antibody (Orange) and Isotype control Antibody (Blue).
Western Blot: beta-Actin Antibody [NB600-503] - Rabbit polyclonal at 1/5000.
Simple Western: beta-Actin Antibody [NB600-503] - Simple Western lane view shows a specific band for Beta Actin in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa more

Product Details

Reactivity Hu, Mu, Rt, Po, Av, ChHa, Fi, Pm, RbSpecies Glossary
Applications WB, Simple Western, B/N, EIA, ICC/IF, IHC, IHC-P, IP, PLA
1.0 mg/ml

beta-Actin Antibody Summary

A synthetic peptide made to an N-terminal region of human Beta Actin. [UniProt P60709].
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-citrate/phosphate, pH 7-8
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified


  • Western Blot 1:100-1:2000
  • Simple Western 1:12.5
  • Blocking/Neutralizing
  • Enzyme Immunoassay
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
  • Proximity Ligation Assay
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Immunocytochemistry/Immunofluorescence, Blocking/Neutralizing and Enzyme Immunoassay were reported in scientific literature. Customer review using ICC. Use in Proximity Ligation Assay reported in scientific literature (PMID 24740405)In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 3 Reviews rated 5
NB600-503 in the following applications:

Read Publications using
NB600-503 in the following applications:

  • B/N
    1 publication
  • PLA
    1 publication
  • WB
    27 publications

Reactivity Notes

Human, Rat, Monkey, Mouse and Avian. Rabbit reactivity reported in the scientific literature (PMID: 23813946). Fish reactivity reported in scientific literature (PMID: 25842206). Expected to cross-react with a wide range of species due to sequence homology.

Alternate Names for beta-Actin Antibody

  • ACTB
  • actin, beta
  • actin, cytoplasmic 1
  • Beta Actin
  • beta cytoskeletal actin
  • betaActin
  • Beta-actin
  • BRWS1
  • PS1TP5-binding protein 1
  • PS1TP5BP1


Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta actin and the static and dynamic aspects of the cytoskeleton.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
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Applications: WB, IHC-P
Species: Hu
Applications: WB, Simple Western
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
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Applications: WB, ICC/IF, IHC, IHC-P
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Applications: WB, ICC/IF, IHC, IHC-P
Species: Multi
Applications: WB, ICC
Species: Hu
Applications: WB
Species: Hu
Applications: PAGE
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, S-ELISA
Species: Hu
Applications: WB
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for beta-Actin Antibody (NB600-503)(71)

We have publications tested in 11 confirmed species: Human, Mouse, Rat, Avian , Chinese Hamster, Fish, Human , Mouse , Porcine, Primate, Rabbit.

We have publications tested in 3 applications: B/N, PLA, WB.

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Chinese Hamster
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Showing Publications 1 - 10 of 71. Show All 71 Publications.
Publications using NB600-503 Applications Species
Lee KH, Nam H, Jeong da E et al. Sensitive Tumorigenic Potential Evaluation of Adult Human Multipotent Neural Cells Immortalized by hTERT Gene Transduction PLoS ONE 2016 Jul 09 [PMID:27391353] (WB, Human) WB Human
Douglas JN, Gardner LA, Salapa HE. Antibodies to the RNA Binding Protein Heterogeneous Nuclear Ribonucleoprotein A1 Colocalize to Stress Granules Resulting in Altered RNA and Protein Levels in a Model of Neurodegeneration in Multiple Sclerosis. J Clin Cell Immunol. Mar 22 2016 12:00AM [PMID:27375925] (WB, Human) WB Human
Savas U, Wei S, Hsu MH et al. 20-Hydroxyeicosatetraenoic Acid (HETE) Dependent Hypertension in Human Cytochrome P450 (CYP) 4A11 Transgenic Mice: Normalization of Blood Pressure by Sodium Restriction, Hydrochlorothiazide, or Blockade of the Type 1 Angiotensin II Receptor. J. Biol. Chem. Jun 13 2016 12:00AM [PMID:27298316] (Mouse) Mouse
Lee H, Lee S, Bae H, Kang HS, Kim SJ. Genome-wide identification of target genes for miR-204 and miR-211 identifies their proliferation stimulatory role in breast cancer cells. 2016 Apr 28 [PMID:27121770] (WB, Human) WB Human
Kramer K, Wu J, Crowe DL. Tumor suppressor control of the cancer stem cell niche. Oncogene. 2015 Dec 21 [PMID:26686086] (WB, Mouse) WB Mouse
Rahman MS, Thomas P. Molecular characterization and hypoxia-induced upregulation of neuronal nitric oxide synthase in Atlantic croaker: Reversal by antioxidant and estrogen treatments Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. 2015 Apr 02 [PMID:25842206] (WB, Fish) WB Fish
Golden D, Cantley LG. Casein kinase 2 prevents mesenchymal transformation by maintaining Foxc2 in the cytoplasm. Oncogene. 2014 Dec 08 [PMID:25486430] (WB, Human, Mouse) WB Human, Mouse
Hsu TM, Konanur VR, Taing L et al. Effects of sucrose and high fructose corn syrup consumption on spatial memory function and hippocampal neuroinflammation in adolescent rats. Hippocampus. 2014 Sep 20 [PMID:25242636] (WB) WB
Wang YW, Kuo CF et al. 2,4,5-trimethoxybenzaldehyde, a bitter principle in plants, suppresses adipogenesis through the regulation of ERK1.. J Agric Food Chem. 2014 Oct 8 [PMID:25222709]
Holden VI, Lenio S, Kuick R et al. Bacterial siderophores that evade or overwhelm Lipocalin 2 induce HIF-1a and pro-inflammatory cytokine secretion in cultured respiratory epithelial cells Infect. Immun. 2014 Jun 30 [PMID:24980968] (WB, Human) WB Human
Show All 71 Publications.

Reviews for beta-Actin Antibody (NB600-503) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.
We have 3 reviews tested in 2 application: ICC, WB.

Reviews using NB600-503:
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Images Ratings Applications Species Date Details
Immunocytochemistry beta-Actin NB600-503
reviewed by:
ICC Human 06/11/2014


Special ApplicationsIF Confocal analysis of HeLa cells using Beta Actin antibody (NB600-503, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green. DAPI was used to stain the cell nuclei (blue).
FileView PDF
Western Blot beta-Actin NB600-503
reviewed by:
WB Human 06/11/2014


ApplicationWestern Blot
Special ApplicationsWestern blot analysis of extracts from Jurkat, COS and CHO cells using Actin antibody (NB600-503, 1:200).
FileView PDF
Western Blot beta-Actin NB600-503
reviewed by:
WB Mouse 11/19/2010


ApplicationWestern Blot
Sample TestedNIH 3T3 cell lysate, Sample Amount: 5 ul


Blocking DetailsBlocking Buffer: 5% Milk in PBST, Blocking Time: 1 hour, Blocking Temp: 20 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Incubation Dilution Buffer: 5% Milk in PBST, Incubation Time: overnight, Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-mouse IgG HRP labeled, Secondary Ab Dilution Ratio: 1:5000
Secondary Manufacturer Cat#NA9310


Detection NotesDetection Method: ECL, Exposure Time: 30 seconds, Specific Bands: around 40 kDa

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta-Actin Antibody (NB600-503). (Showing 1 - 5 of 5 FAQs).

  1. Why is beta actin used as a loading control in blotting ?
    • Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
  2. Are the beta-Actin antibodies validated in Simple Western?
    • Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
  3. Do Beta-Actin antibodies come in lyophilized format?
    • ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
  4. What the theoretical molecular weight for Beta-Actin antibodies?
    • The TMW of beta Actin antibodies is approximately 40 - 42 kDa.
  5. What is the immunogen sequence of this Beta-Actin antibody?
    • A sequence within the N-terminal region of Human beta Actin. The exact sequence is proprietary.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB600-503AF405
Alexa Fluor (R) 488 Labeled NB600-503AF488
Alexa Fluor (R) 647 Labeled NB600-503AF647
Alexa Fluor (R) 700 Labeled NB600-503AF700
Biotin Labeled NB600-503B
DyLight 405 Labeled NB600-503V
DyLight 488 Labeled NB600-503G
DyLight 550 Labeled NB600-503R
DyLight 650 Labeled NB600-503C
FITC Labeled NB600-503F
HRP Labeled NB600-503H

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Blogs on beta-Actin.

Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...  Read full blog post.

Beta-Actin's Role in Neuronal Plasticity
Beta-Actin is a highly conserved protein involved in cell growth, cytoskeletal and extracellular support structures and cell migration. Because beta-Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading contro...  Read full blog post.

ACTB - an abundant cytoskeletal component with applications for gene expression analysis
Actin is the widely studied and ubiquitous cytoskeletal protein capable of forming dynamic microfilament structures. These filaments are essential for diverse cellular functions including cell shape, migration, cytokinesis, and intracellular traffi...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol ACTB

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