Recombinant Human Ubiquitin Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human Ubiquitin Protein, CF Summary

Details of Functionality
Recombinant Human Ubiquitin can be conjugated to substrate proteins via the subsequent actions of a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin concentration of 0.01-0.5 mM.
Source
E. coli-derived human Ubiquitin protein
Accession #
Protein/Peptide Type
Recombinant Proteins
Gene
UBB
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

Theoretical MW
8.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
U-100H in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a solution in deionized water.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Reconstitution Instructions
Reconstitute at 10 mg/mL in an aqueous solution.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Ubiquitin Protein, CF

  • RPS27A
  • UBA52
  • UBB ubiquitin B
  • UBB
  • UBC
  • Ubiquitin

Background

Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin (1). In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1) (2,3). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting (4-7).

Highly purified Ubiquitin processed for the quantitative removal of glycine and buffer salts which can interfere with chemical and in vitro reactions.

  1. Sharp, P.M. & W.-H. Li. (1987) Trends Ecol. Evol. 2:328.
  2. Ciechanover, A. et al. (1980 ) Proc. Natl. Acad. Sci. USA 77:1365.
  3. Hershko, A. et al. (1980) Proc. Natl. Acad. Sci. USA 77:1783.
  4. Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
  5. Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
  6. Wei, W. et al. (2004) Nature 428:194.
  7. Wertz, I.E. et al. (2004) Nature 430:694.

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U-100H
Species: Hu
Applications: Enzyme Activity

Publications for Ubiquitin (U-100H)(54)

We have publications tested in 4 confirmed species: Human, Bacteria - E. Coli, N/A, Saccharomyces cerevisiae.

We have publications tested in 8 applications: Binding Assay, Bioassay, ELISA, EnzAct, Enzyme Assay, Ubiquitination, enzymatic assay, in vitro ubiquitination.


Filter By Application
Binding Assay
(1)
Bioassay
(44)
ELISA
(1)
EnzAct
(1)
Enzyme Assay
(1)
Ubiquitination
(3)
enzymatic assay
(1)
in vitro ubiquitination
(1)
All Applications
Filter By Species
Human
(25)
Bacteria - E. Coli
(2)
N/A
(18)
Saccharomyces cerevisiae
(1)
All Species
Showing Publications 1 - 10 of 54. Show All 54 Publications.
Publications using U-100H Applications Species
S Ito, A Ueno, T Ueda, H Nakagawa, H Taniguchi, N Kayukawa, A Fujihara-I, F Hongo, K Okihara, O Ukimura CNPY2 inhibits MYLIP-mediated AR protein degradation in prostate cancer cells Oncotarget, 2018;9(25):17645-17655. 2018 [PMID: 29707137] (Bioassay) Bioassay
D Valleau, AT Quaile, H Cui, X Xu, E Evdokimova, C Chang, ME Cuff, ML Urbanus, S Houliston, CH Arrowsmith, AW Ensminger, A Savchenko Discovery of Ubiquitin Deamidases in the Pathogenic Arsenal of Legionella pneumophila Cell Rep, 2018;23(2):568-583. 2018 [PMID: 29642013] (Bioassay) Bioassay
LJ Albee, HM LaPorte, X Gao, JM Eby, YH Cheng, AM Nevins, BF Volkman, V Gaponenko, M Majetschak Identification and functional characterization of arginine vasopressin receptor 1A : atypical chemokine receptor 3 heteromers in vascular smooth muscle Open Biol, 2018;8(1):. 2018 [PMID: 29386406] (Bioassay, Human) Bioassay Human
SY Kim, HJ Kim, HJ Kim, DH Kim, CH Kim, JH Han, HK Byeon, K Lee HSPA5 negatively regulates lysosomal activity through ubiquitination of MUL1 in head and neck cancer Autophagy, 2017;0(0):1-89. 2017 [PMID: 29260979] (Bioassay, Human) Bioassay Human
C Yang, W Zang, Z Tang, Y Ji, R Xu, Y Yang, A Luo, B Hu, Z Zhang, Z Liu, X Zheng A20 regulates the DNA damage response and mediates tumor cell resistance to DNA damaging therapy Cancer Res., 2017;0(0):. 2017 [PMID: 29233925] (Bioassay, Human) Bioassay Human
JB Hein, EPT Hertz, DH Garvanska, T Kruse, J Nilsson Distinct kinetics of serine and threonine dephosphorylation are essential for mitosis Nat. Cell Biol., 2017;19(12):1433-1440. 2017 [PMID: 29084198] (Bioassay, Human) Bioassay Human
M Boselli, BH Lee, J Robert, MA Prado, SW Min, C Cheng, MC Silva, C Seong, S Elsasser, KM Hatle, TC Gahman, SP Gygi, SJ Haggarty, L Gan, RW King, D Finley An inhibitor of the proteasomal deubiquitinating enzyme USP14 induces tau elimination in cultured neurons J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28972160] (EnzAct, Human) EnzAct Human
S Wang, J Juan, Z Zhang, Y Du, Y Xu, J Tong, B Cao, MF Moran, Y Zeng, X Mao Inhibition of the deubiquitinase USP5 leads to c-Maf protein degradation and myeloma cell apoptosis Cell Death Dis, 2017;8(9):e3058. 2017 [PMID: 28933784] (Bioassay, Human) Bioassay Human
BA Osuna, CJ Howard, S Kc, A Frost, DE Weinberg In vitro analysis of RQC activities provides insights into the mechanism and function of CAT tailing Elife, 2017;6(0):. 2017 [PMID: 28718767] (Bioassay, Saccharomyces cerevisiae) Bioassay Saccharomyces cerevisiae
Y Kagami, M Ono, K Yoshida Plk1 phosphorylation of CAP-H2 triggers chromosome condensation by condensin II at the early phase of mitosis Sci Rep, 2017;7(1):5583. 2017 [PMID: 28717250] (Bioassay, Human) Bioassay Human
Show All 54 Publications.

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Blogs on Ubiquitin. Showing 1-10 of 343 blog posts - Show all blog posts.

There's an autophagy for that!
By Christina Towers, PhDA critical mechanism that cells use to generate nutrients and fuel metabolism is through a process called autophagy.  This process is complex and involves over 20 different proteins, most of which are highly conserved acro...  Read full blog post.

There's an autophagy for that!
By Christina Towers, PhDA critical mechanism that cells use to generate nutrients and fuel metabolism is through a process called autophagy.  This process is complex and involves over 20 different proteins, most of which are highly conserved acro...  Read full blog post.

There's an autophagy for that!
By Christina Towers, PhDA critical mechanism that cells use to generate nutrients and fuel metabolism is through a process called autophagy.  This process is complex and involves over 20 different proteins, most of which are highly conserved acro...  Read full blog post.

There's an autophagy for that!
By Christina Towers, PhDA critical mechanism that cells use to generate nutrients and fuel metabolism is through a process called autophagy.  This process is complex and involves over 20 different proteins, most of which are highly conserved acro...  Read full blog post.

There's an autophagy for that!
By Christina Towers, PhDA critical mechanism that cells use to generate nutrients and fuel metabolism is through a process called autophagy.  This process is complex and involves over 20 different proteins, most of which are highly conserved acro...  Read full blog post.

There's an autophagy for that!
By Christina Towers, PhDA critical mechanism that cells use to generate nutrients and fuel metabolism is through a process called autophagy.  This process is complex and involves over 20 different proteins, most of which are highly conserved acro...  Read full blog post.

There's an autophagy for that!
By Christina Towers, PhDA critical mechanism that cells use to generate nutrients and fuel metabolism is through a process called autophagy.  This process is complex and involves over 20 different proteins, most of which are highly conserved acro...  Read full blog post.

There's an autophagy for that!
By Christina Towers, PhDA critical mechanism that cells use to generate nutrients and fuel metabolism is through a process called autophagy.  This process is complex and involves over 20 different proteins, most of which are highly conserved acro...  Read full blog post.

There's an autophagy for that!
By Christina Towers, PhDA critical mechanism that cells use to generate nutrients and fuel metabolism is through a process called autophagy.  This process is complex and involves over 20 different proteins, most of which are highly conserved acro...  Read full blog post.

There's an autophagy for that!
By Christina Towers, PhDA critical mechanism that cells use to generate nutrients and fuel metabolism is through a process called autophagy.  This process is complex and involves over 20 different proteins, most of which are highly conserved acro...  Read full blog post.

Showing 1-10 of 343 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol UBB
Entrez
Uniprot