Cyclophilin A Antibody

Images

 
Western Blot: Cyclophilin A Antibody [NBP1-30993] - Raji whole cell extracts and cytoplasma+membrane and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody ...read more
Immunocytochemistry/ Immunofluorescence: Cyclophilin A Antibody [NBP1-30993] - Methanol-fixed HeLa, using Cyclophilin A antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Cyclophilin A Antibody [NBP1-30993] - Paraffin-embedded MDA-MB-468 xenograft, using antibody at 1:500 dilution.
Western Blot: Cyclophilin A Antibody [NBP1-30993] - (30 ug of whole cell lysate) A: H1299 B: HeLa S3 12% SDS PAGE, antibody diluted at 1:5000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Fe, Pm, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Cyclophilin A Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Cyclophilin A. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Feline (100%), Sheep (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPIA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 40% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cyclophilin A Antibody

  • Cyclophilin A
  • Cyclosporin A-binding protein
  • CYPA
  • CYPAMGC12404
  • CYPH
  • EC 5.2.1.8
  • MGC117158
  • MGC23397
  • peptidyl-prolyl cis-trans isomerase A
  • peptidylprolyl isomerase A (cyclophilin A)
  • PPIA
  • PPIase A
  • Rotamase A
  • Rotamase
  • T cell cyclophilin

Background

This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PPIA
Entrez