Western Blot: Cyclophilin A Antibody [NBP1-30993] - (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE diluted at 1:3000
Immunocytochemistry/ Immunofluorescence: Cyclophilin A Antibody [NBP1-30993] - Methanol-fixed HeLa, using Cyclophilin A antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Cyclophilin A Antibody [NBP1-30993] - MDA-MB-468 xenograft, using Cyclophilin A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Orthogonal Strategies: Western Blot: Cyclophilin A Antibody [NBP1-30993] - Raji whole cell extracts and cytoplasma+membrane and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was ...read more
Western Blot: Cyclophilin A Antibody [NBP1-30993] - Analysis. Mouse tissue extracts (50 ug) was separated by 15 % SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody at a dilution of 1:20000.
Knockdown Validated: Cyclophilin A Antibody [NBP1-30993] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cyclophilin A ...read more
18 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBP1-30993 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.1M Tris-Glycine (pH7), 20% Glycerol
Alternate Names for Cyclophilin A Antibody
Cyclosporin A-binding protein
peptidyl-prolyl cis-trans isomerase A
peptidylprolyl isomerase A (cyclophilin A)
T cell cyclophilin
This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
Publications for Cyclophilin A Antibody (NBP1-30993)(2)
We have publications tested in 1 confirmed species: Rat.
We have publications tested in 1 application: IF/IHC.
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