NIH 3T3 Whole Cell Lysate Summary
NIH/3T3 whole cell lysate (Positive control)
This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Standard RIPA buffer including protease and phosphatase inhibitors and 0.1% SDS diluted in 1 X lithium dodecyl sulfate (LDS) sample buffer with bromophenol blue and 40-70% glycerol, pH 8.4. BME has not been added.
Lysate Details for Array
NIH3T3 cells are mouse embryonic fibroblast cells that are derived from Swiss mouse embryonic tissue. 3T3 is an abbreviation of 3-day transfer, inoculum 3 x 10^5 cells. NIH3T3 cells are used primarily in the cultivation of keratinocytes.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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