NIH 3T3 Whole Cell Lysate

Images

 
Western Blot: NIH 3T3 Whole Cell Lysate [NB800-PC8] - Analysis of GAPDH (NB300-323) using NIH3T3 whole cell lysate [NB800-PC8].
SDS-Page: NIH 3T3 Whole Cell Lysate [NB800-PC8] - Ponceau S staining of NIH 3T3 whole cell lysate using NB800-PC8.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, PAGE
Concentration
2.5 mg/ml

Order Details

NIH 3T3 Whole Cell Lysate Summary

Specificity
NIH/3T3 whole cell lysate (Positive control)

Applications/Dilutions

Application Notes
This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Standard RIPA buffer including protease and phosphatase inhibitors and 0.1% SDS diluted in 1 X lithium dodecyl sulfate (LDS) sample buffer with bromophenol blue and 40-70% glycerol, pH 8.4. BME has not been added.
Preservative
No Preservative
Concentration
2.5 mg/ml

Lysate Details for Array

Type
Cell
Tissue
Bone/Cartilage
Subcellular Fraction
Whole

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NIH 3T3 Lysate (NB800-PC8) (0)

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Other Available Formats

NB800-PC8

Additional NIH 3T3 Products

Array NB800-PC8

Blogs on NIH 3T3

There are no specific blogs for NIH 3T3, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

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