EIF3B Antibody

Images

 
Western Blot: EIF3B Antibody [NB100-93302] - Whole cell lysate (15 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF3B antibody used for WB at 0.1 ug/ml. ...read more
Immunohistochemistry-Paraffin: EIF3B Antibody [NB100-93302] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit antiEIF3S9/eIF3B used at a dilution of 1:200 (1ug/ml). Detection: DAB
Independent Antibodies: Western Blot: EIF3B Antibody [NB100-93302] - Detection of Human EIF3S9/eIF3B on HeLa whole cell lysate using NB100-93302. EIF3S9/eIF3B was also immunoprecipitated by rabbit ...read more
Independent Antibodies: Immunoprecipitation: EIF3B Antibody [NB100-93302] - Detection of human eIF3B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

EIF3B Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human eukaryotic translation initiation factor 3, subunit B (eukaryotic translation initiation factor 3, subunit 9) using the numbering given in entry NP_003742.2 (G
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF3B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:250
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Publications
Read Publications using
NB100-93302 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EIF3B Antibody

  • eIF3 p110
  • eIF3 p116
  • eIF3b
  • eIF-3-eta
  • EIF3-P110
  • EIF3-P116
  • EIF3S9EIF3-ETA
  • Eukaryotic translation initiation factor 3 subunit 9
  • eukaryotic translation initiation factor 3 subunit B
  • eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD)
  • eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa
  • eukaryotic translation initiation factor 3, subunit B
  • MGC104664
  • MGC131875
  • Prt1 homolog
  • PRT1hPrt1

Background

Eukaryotic initiation factor 3 subunit B (eIF3B) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5'-end of cellular mRNAs near the cap structure (m7GpppN). eIF3B is a conserved subunit and part of the functional core of eIF3. It bears a non-canonical RNA recognition motif (RRM) and interacts directly with the eIF3S1 subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EIF3B Antibody (NB100-93302)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol EIF3B
Entrez
Uniprot