beta-Actin Antibody [Alexa Fluor® 647]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Av, ChHa, Fi, Pm, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Alexa Fluor 647

beta-Actin Antibody [Alexa Fluor® 647] Summary

Immunogen
A synthetic peptide made to an N-terminal region of human Beta Actin. [UniProt P60709].
Localization
Cytoplasmic
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACTB
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This Beta Actin antibody is useful for Western Blotting, Immunoprecipitation, and Immunohistochemistry -paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Immunocytochemistry testing in HeLa and NIH3T3 cells did not produce any positive staining. Immunocytochemistry/Immunofluorescence, Blocking/Neutralizing and Enzyme Immunoassay were reported in scientific literature.
Reviewed Applications
Read 1 Review rated 4
using
NB600-503AF647 in the following applications:

Reactivity Notes

Human, Rat, Monkey, Mouse and Avian. Rabbit reactivity reported in the scientific literature (PMID: 23813946). Fish reactivity reported in scientific literature (PMID: 25842206). Expected to cross-react with a wide range of species due to sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for beta-Actin Antibody [Alexa Fluor® 647]

  • ACTB
  • actin, beta
  • actin, cytoplasmic 1
  • Beta Actin
  • beta cytoskeletal actin
  • betaActin
  • beta-Actin
  • BRWS1
  • PS1TP5-binding protein 1
  • PS1TP5BP1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for beta-Actin Antibody (NB600-503AF647) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IF.

Reviews using NB600-503AF647:
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Images Ratings Applications Species Date Details
Immunofluorescence beta-Actin NB600-503AF647
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reviewed by:
Anonymous
IF Mouse 05/11/2016
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Summary

ApplicationImmunofluorescence
Sample TestedMouse Primary Melanocytes
SpeciesMouse
LotA10-041416-AF647

Blocking

Blocking Details2% BSA in PBS with 0.1% Triton X-100

Primary Anitbody

Dilution Ratio2% BSA in PBS with 0.1% Triton X-100 and 0.1% Azide

Details

Detection NotesEVOS FL Auto Cy5 cube. Wells with no antibody had no staining/fluorescence.
Fixation DetailsPlated cells on chamber slide, Fixed with 4% Paraformaldehyde 15 min @ room temp, permeabilized with 0.5% Triton-X100 in PBS.
Wash DescriptionWash 3x with Buffer between each step (Buffer: PBS + 0.1% Triton-X )

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta-Actin Antibody (NB600-503AF647). (Showing 1 - 5 of 5 FAQ).

  1. Why is beta actin used as a loading control in blotting ?
    • Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
  2. Are the beta-Actin antibodies validated in Simple Western?
    • Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
  3. Do Beta-Actin antibodies come in lyophilized format?
    • ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
  4. What the theoretical molecular weight for Beta-Actin antibodies?
    • The TMW of beta Actin antibodies is approximately 40 - 42 kDa.
  5. What is the immunogen sequence of this Beta-Actin antibody?
    • A sequence within the N-terminal region of Human beta Actin. The exact sequence is proprietary.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 700 Labeled NB600-503AF700

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Discover related pathways, diseases and genes to beta-Actin Antibody (NB600-503AF647). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for beta-Actin Antibody (NB600-503AF647)

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Research Areas for beta-Actin Antibody (NB600-503AF647)

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Blogs on beta-Actin.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...  Read full blog post.

Beta-Actin's Role in Neuronal Plasticity
Beta-Actin is a highly conserved protein involved in cell growth, cytoskeletal and extracellular support structures and cell migration. Because beta-Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading contro...  Read full blog post.

ACTB - an abundant cytoskeletal component with applications for gene expression analysis
Actin is the widely studied and ubiquitous cytoskeletal protein capable of forming dynamic microfilament structures. These filaments are essential for diverse cellular functions including cell shape, migration, cytokinesis, and intracellular traffi...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
05/11/2016
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol ACTB
Entrez