GAPDH Antibody (1D4)

Images

 
Western Blot: GAPDH Antibody (1D4) [NB300-221] - analysis of GAPDH in human nucleus pulposus cell culture (40ug/lane) using anti-GAPDH antibody. LI-COR Odyssey imaging system was used for detection. Image from verified ...read more
Immunocytochemistry/ Immunofluorescence: GAPDH Antibody (1D4) [NB300-221] - Analysis of of HeLa cells stained with GAPDH antibody, dilution 1:100 (Green). DAPI staining of nuclear DNA (Blue). The GAPDHantibody produces ...read more
Western Blot: GAPDH Antibody (1D4) [NB300-221] - Analysis of cell line lysates probed with mouse mAb to GAPDH (theoretical molecular weight: 36 kDa), NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] ...read more
Western Blot: GAPDH Antibody (1D4) [NB300-221] - analysis of GAPDH in chicken pectoralis major using anti-GAPDH antibody. The primary antibody was used at a dilution of 1:4000, incubated for 1 hour at room temperature. ...read more
Immunocytochemistry/ Immunofluorescence: GAPDH Antibody (1D4) [NB300-221] - SH-SY5Y cells stained with GAPDH antibody NB300-221 (green). Nuclear DNA is stained with Hoechst dye (blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Eq, Fe, Ha, Op, Pm, Rb, Sh, ZeSpecies Glossary
Applications WB, Simple Western, ChIP, ICC/IF, IHC, IHC-Fr, IP, KD, Single Cell Western
Clone
1D4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

GAPDH Antibody (1D4) Summary

Description
This GAPDH antibody (1D4) is primarily used as a loading control in western blots due to the near global expression of GAPDH which can be attributed to its vital role in the glycolytic metabolic process.
Immunogen
This GAPDH antibody was developed against full length purified porcine GAPDH [UniProt# P00355]
Marker
Cytosolic Marker
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
GAPDH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:25
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Knockdown Validated
  • Single Cell Western
Application Notes
Use in IP, ChIP, and IHC-Fr reported in scientific literature (PMID: 24032460, 25802280, and 20538044 respectively).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
GAPDH Overexpression Lysate
Reviewed Applications
Read 17 Reviews rated 4.4
using
NB300-221 in the following applications:

Publications
Read Publications using
NB300-221 in the following applications:

Reactivity Notes

Human, mouse, rat, bovine, porcine, avian, sheep, opossum and primate. Likely to react with other mammals as well. Hamster, zebrafish and feline reactivity reported by customer reviews. Chicken, rabbit, and Chinese hamster reactivity reported in scientific literature (PMID: 24725420, 17545481, and 24928958 respectively).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50% PBS, 50% Glycerol
Preservative
5mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GAPDH Antibody (1D4)

  • 1D4 GAPDH
  • aging-associated gene 9 protein
  • Cytoplasm Marker
  • EC 1.2.1
  • EC 1.2.1.12
  • EC 2.6.99.-
  • G3PD antibody
  • G3PD
  • G3PDH
  • GAPD
  • GAPDH
  • GAPDPeptidyl-cysteine S-nitrosylase GAPDH antibody
  • glyceraldehyde 3-phosphate dehydrogenase
  • glyceraldehyde-3-phosphate dehydrogenase
  • MGC102544
  • MGC102546
  • MGC103190
  • MGC103191
  • MGC105239
  • MGC127711
  • MGC88685
  • OCAS, p38
  • OCT1 coactivator in S phase
  • Peptidyl-cysteine S-nitrosylase GAPDH

Background

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a ubiquitous enzyme involved in glycolysis, converting glyceraldehyde-3-phosphate into 1,3 diphosphoglycerate. This constitutively expressed, homotetramer protein can be found in the nucleus and cytoplasm, and the monomer has a theoretical molecular weight of 36 kDa. Known as a housekeeping gene, GAPDH routinely serves as a control for real-time PCR (RT-PCR) and as a loading control for Western Blots (1-3). In addition to its role in glycolysis, GAPDH has multiple cellular functions including membrane trafficking, transcription activation, apoptosis, DNA repair, and has been implicated in various pathologies such as cancer and neurodegenerative diseases including Alzheimer's and Huntington's (4,5).

References
1) Barber RD, Harmer DW, Coleman RA, Clark BJ. (2005) GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues. Physiol Genomics. 21(3):389-95. PMID: 15769908

2) Jia Y, Takimoto K. (2006) Mitogen-activated protein kinases control cardiac KChIP2 gene expression. Circ Res. 98(3):386-93. PMID: 16385079

3) Godsel LM, Hsieh SN, Amargo EV, Bass AE, Pascoe-McGillicuddy LT, Huen AC, Thorne ME, Gaudry CA, Park JK, Myung K, Goldman RD, Chew TL, Green KJ. (2005) Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. J Cell Biol. 171(6):1045-59. PMID: 16365169

4) Sirover MA1. (1999) New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase. Biochim Biophys Acta. 1432(2): 159-84. PMID: 10407139

5) Tristan C, Shahani N, Sedlak TW, Sawa A. (2011) The diverse functions of GAPDH: views from different subcellular compartments. Cell Signal. 23(2):317-23. PMID: 20727968

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB300-221
Species: Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Eq, Fe, Ha, Op, Pm, Rb, Sh, Ze
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IHC-Fr, IP, KD, Single Cell Western

Publications for GAPDH Antibody (NB300-221)(282)

We have publications tested in 13 confirmed species: Human, Mouse, Rat, Bovine, Canine, Chicken, Chinese Hamster, Human , Opossum, Porcine, Primate, Rabbit, Sheep.

We have publications tested in 7 applications: ChIP, ICC/IF, IHC, IHC-Fr, IP, Single Cell Western, WB.


Filter By Application
ChIP
(1)
ICC/IF
(7)
IHC
(2)
IHC-Fr
(1)
IP
(3)
Single Cell Western
(1)
WB
(246)
All Applications
Filter By Species
Human
(128)
Mouse
(83)
Rat
(36)
Bovine
(2)
Canine
(1)
Chicken
(4)
Chinese Hamster
(1)
Human
(1)
Opossum
(1)
Porcine
(4)
Primate
(1)
Rabbit
(1)
Sheep
(4)
All Species
Showing Publications 1 - 10 of 282. Show All 282 Publications.
Publications using NB300-221 Applications Species
Zhao N. Notch Signaling Guides Vascular Smooth Muscle Cell Function. Thesis. 2014 (WB, Human, Mouse) WB Human, Mouse
Kovalchuke L, Mosharov EV, Levy OA, Greene LA Stress-induced phospho-ubiquitin formation causes parkin degradation Sci Rep Aug 12 2019 [PMID: 31406131] (WB, Rat) WB Rat
Gunasekar P, Satish M, Dabestani P et al. Modulation of Cathepsin L Expression in the Coronary Arteries of Atherosclerotic Swine Journal of Surgical Research Nov 1 2019 [PMID: 31377485] (WB, Porcine) WB Porcine
Crawley JTB, Zalli A, Monkman JH et al. Defective fibrin deposition and thrombus stability in Bambi-/- mice is mediated by elevated anticoagulant function J. Thromb. Haemost. Jul 27 2019 [PMID: 31351019] (WB, Mouse) WB Mouse
Gordon SM, Nishiguchi MA, Mani S, Mainigi MA Interferons drive development of novel interleukin-15-responsive macrophages in the uterus bioRxiv 2019 Jun 06 (WB, Human ) WB Human
Aggarwal M, Saxena R, Asif N et al. p53 mutant-type in human prostate cancer cells determines the sensitivity to phenethyl isothiocyanate induced growth inhibition J. Exp. Clin. Cancer Res. Jul 15 2019 [PMID: 31307507] (IP, WB, Human, Mouse) IP, WB Human, Mouse
Penas C, Maloof ME, Stathias V et al. Time series modeling of cell cycle exit identifies Brd4 dependent regulation of cerebellar neurogenesis Nat Commun Jul 10 2019 [PMID: 31292434] (WB, Mouse) WB Mouse
Epp AL, Ebert SN, Sanchez-Arias JC et al. A novel motif in the proximal C-terminus of Pannexin 1 regulates cell surface localization Sci Rep Jul 5 2019 [PMID: 31278290] (WB, Human) WB Human
Yan S, Zhou J, Chen X et al. Diverse Consequences in Liver Injury in Mice with Different Autophagy Functional Status Treated with Alcohol Am. J. Pathol. Jun 11 2019 [PMID: 31199920] (Mouse) Mouse
Hans CP, Sharma N, Sen S et al. Transcriptomics Analysis Reveals New Insights into the Roles of Notch1 Signaling on Macrophage Polarization Sci Rep May 29 2019 [PMID: 31142802] (WB, Mouse) WB Mouse
Show All 282 Publications.

Reviews for GAPDH Antibody (NB300-221) (17) 4.417

Average Rating: 4.4
(Based on 17 reviews)
We have 17 reviews tested in 6 species: Human, Mouse, Rat, Mouse and Human, Other, cat.

Reviews using NB300-221:
Filter by Applications
WB
(17)
All Applications
Filter by Species
Human
(6)
Mouse
(2)
Rat
(3)
Mouse and Human
(1)
Other
(4)
cat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot GAPDH NB300-221
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reviewed by:
Wan-Ching Cheng
WB cat 09/13/2018
View

Summary

ApplicationWestern Blot
Sample TestedHeart tissue
Speciescat
Lot083017
Western Blot GAPDH NB300-221
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reviewed by:
Anonymous
WB Mouse and Human 06/29/2017
View

Summary

ApplicationWestern Blot
Sample Tested293FT cell lysate,nih3t3 cell lysate
SpeciesMouse and Human
Lot120116
Western Blot GAPDH NB300-221
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reviewed by:
Jim Hsiao
WB Mouse 05/22/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse lung endothelial cells
SpeciesMouse
Lot012517

Comments

CommentsCell lysates of treated mouse lung endothelial cells were analyzed by western blotting.
Western Blot GAPDH NB300-221
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reviewed by:
Anonymous
WB Human 01/14/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman nucleus pulposus cell culture
SpeciesHuman
Lot111711

Blocking

Blocking DetailsLi-Cor WB blocking buffer at 1X for 1h at room temperature.

Primary Anitbody

Dilution Ratio1:500 dilution. Incubate overnight at 4 degree with gently shaking. Buffer: 50% of Li-cor blocking buffer plus 50% of PBS.

Secondary Antibody

Secondary DescriptionGOAT-anti-mouse IRDye 680
Secondary Manufacturer Cat#Li-Cor #926-32220
Secondary Concentration1:10000

Details

Detection NotesLi-Cor Odyssey. Membrane scan mode. postive control is A431 cell lysate. negative control is untreated cells.

Comments

CommentsThis is product works similar to the other GAPDH we had in this experiment. Backgroud is very little.
Western Blot GAPDH NB300-221
Enlarge
reviewed by:
Anonymous
WB Other 01/09/2015
View

Summary

ApplicationWestern Blot
Sample TestedChicken Pectoralis Major
SpeciesOther
Lot111711

Blocking

Blocking Details3% Casein in PBS with 0.1% Tween-20 for 1 hr

Primary Anitbody

Dilution Ratio1/4000 at Room Temperature for 1 hr

Secondary Antibody

Secondary DescriptionGoat anti-Mouse 680 RD - Licor Biosciences
Secondary Manufacturer Cat#926-68180
Secondary Concentration1/25,000

Details

Detection NotesLicor Odyssey
Western Blot GAPDH NB300-221
Enlarge
reviewed by:
Eric England
WB Other 10/08/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole Skeletal Muscle Lysate
SpeciesOther
Lot111711

Blocking

Blocking Details3% Casein in PBS with 0.1% Tween-20 for 1 hr

Primary Anitbody

Dilution Ratio1/2000 at 4C for 18 hr

Secondary Antibody

Secondary DescriptionGoat anti-Mouse 680 RD - Licor Biosciences
Secondary Manufacturer Cat#926-68180
Secondary Concentration1/25,000

Details

Detection NotesLicor Odyssey
Western Blot GAPDH NB300-221
Enlarge
reviewed by:
Liyong Zhang
WB Mouse 07/01/2014
View

Summary

ApplicationWestern Blot
SpeciesMouse
FileView PDF
Western Blot GAPDH NB300-221
Enlarge
reviewed by:
Anonymous
WB Other 12/16/2013
View

Summary

ApplicationWestern Blot
Sample TestedZebrafish embryo lysates
SpeciesOther
CommentsZebrafish embryo lysates at different developmental stages probed with GAPDH antibody 1D4.

Comments

CommentsZebrafish embryo lysates at different developmental stages probed with GAPDH antibody 1D4.
 
reviewed by:
Anonymous
WB Human 12/23/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:2000, Primary Ab Incubation Time: overnight

Secondary Antibody

Secondary DescriptionSecondary Ab: Anti-Mouse HRP

Details

Detection NotesDetection Method: ECL
 
reviewed by:
Anonymous
WB Human 12/21/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman Hepatoblastoma
SpeciesHuman
CommentsThe cultured cells were harvested, washed and lysed. Extracts were stored at -20C prior to analysis

Blocking

Blocking DetailsBlocking Buffer: 5% non-fat milk in PBST, Blocking Time: 1 hour

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: 24 hours, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat anti-Mouse IgG (HRP), Jackson

Details

Detection NotesDetection Method: GE ImageQuant LAS 4000 mini plus ECL

Comments

CommentsThe cultured cells were harvested, washed and lysed. Extracts were stored at -20C prior to analysis
 
reviewed by:
Anonymous
WB Rat 12/06/2011
View

Summary

ApplicationWestern Blot
Sample TestedRat
SpeciesRat

Blocking

Blocking DetailsBlocking Buffer: 5% Milk

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: 5 hours

Secondary Antibody

Secondary DescriptionSecondary Ab: Mouse

Details

Detection NotesDetection Method: ECL
 
reviewed by:
Anonymous
WB Human 12/05/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: Milk

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab Dilution Ratio: 1:5000

Details

Detection NotesDetection Method: ECL
 
reviewed by:
Anonymous
WB Rat 11/23/2011
View

Summary

ApplicationWestern Blot
Sample TestedRat brain
SpeciesRat
CommentsGood!

Blocking

Blocking DetailsBlocking Buffer: 1% milk, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1500, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-mouse, Secondary Ab Dilution Ratio: 1:2000

Details

Detection NotesDetection Method: ECL

Comments

CommentsGood!
 
reviewed by:
Anonymous
WB Rat 11/21/2011
View

Summary

ApplicationWestern Blot
Sample TestedRat
SpeciesRat
Commentsok

Blocking

Blocking DetailsBlocking Buffer: 5% non-fat milk in TBST

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight

Secondary Antibody

Secondary DescriptionSecondary Ab: AP-conjugate antibody

Comments

Commentsok
 
reviewed by:
Anonymous
WB Human 09/30/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman
CommentsVery good!

Blocking

Blocking DetailsBlocking Buffer: 5% milk in 0.1% PBST

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000 diluted in blocking solution, Primary Ab Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab Dilution Ratio: 1:5000 diluted in blocking solution

Details

Detection NotesDetection Method: ECL

Comments

CommentsVery good!
 
reviewed by:
Anonymous
WB Other 03/25/2010
View

Summary

ApplicationWestern Blot
Sample TestedCHO cells, Sample Amount: 20ug
SpeciesOther
Lot113003

Blocking

Blocking DetailsBlocking Buffer: 5% milk TBS/T, Blocking Time: 1 hour, Blocking Temp: 20 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Incubation Dilution Buffer: 5% milk TBS/T, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: goat anti-mouse mAb, conj. HRP
 
reviewed by:
Anonymous
WB Human 06/04/2009
View

Summary

ApplicationWestern Blot
Sample TestedEAhy926 cell lysate, Sample Amount: 15ug
SpeciesHuman
LotP1
CommentsWe've been using this product for many years.

Blocking

Blocking DetailsBlocking Buffer: StartingBlock, Blocking Time: 2 hours, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1/5000, Incubation Dilution Buffer: 1:5 StartingBlock to Water, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-mouse, Secondary Ab Dilution Ratio: 1/50000

Details

Detection NotesDetection Method: ECL, Exposure Time: 2 seconds, Specific Bands: 30-40 kDa

Comments

CommentsWe've been using this product for many years.

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for GAPDH Antibody (NB300-221). (Showing 1 - 6 of 6 FAQs).

  1. We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?
    • GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.
  2. I want to know why GAPDH is used as a loading control antibody in WB techniques.
    • Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.
  3. I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?
    • This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.
  4. I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?
    • Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.
  5. What secondary antibody should I use for visualization of the GAPDH antibody?
    • This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.
  6. Do you offer a smaller size for the GAPDH antibodies?
    • We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for GAPDH Antibody (NB300-221)

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Blogs on GAPDH. Showing 1-10 of 13 blog posts - Show all blog posts.

Considerations for Quantitative Western blotting
By Jamshed Arslan, Pharm. D., PhD. Since its inception in 1979, Western blotting has undergone several developments. The use of radioactive probes was common throughout 1980s, but utilizing secondary antibodies labell...  Read full blog post.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...  Read full blog post.

GAPDH - A "housekeeping" gene with diverse functions in cellular homeostasis
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a well-known housekeeping gene with functions in glycolysis. Many biologists are familiar with the gene and use GAPDH antibodies for a loading control when performing western blots. However, this...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

GAPDH: More than a housekeeping gene
GAPDH is a 146kD tetramer glycolytic pathway metabolic enzyme composed of four 30-40 kDa subunits. It is responsible for reversibly phosphorylating its substrate glyceraldehyde 3-phosphate within the glycolytic pathway.  Apart from its role in gl...  Read full blog post.

GAPDH (Glyceraldehyde 3-Phosphate Dehydrogenase)
GAPDH is a 146 kD tetramer glycolytic pathway metabolic enzyme responsible for reversibly phosphorylating glyceraldehyde 3-phosphate. It may have other possible functions in transcriptional activation. GAPDH is highly expressed due to this housekeepin...  Read full blog post.

Time to Start Actin Like a Reliable 'Housekeeper'!
A growing body of data and studies using actin antibodies supports a view of the actin cytoskeleton of smooth muscle cells as a dynamic structure that plays an integral role in regulating the development of mechanical tension and the material properti...  Read full blog post.

Beta Actin and GAPDH: The Importance of Loading Controls
Western blotting is an essential technique to probe protein expression in complex cell or tissue lysates. To accurately determine protein expression and interpret Western blot results, it is important to use loading controls. A loading control anti...  Read full blog post.

The GAPDH Antibody in Western blot Assays
The loading controls on our antibody database are widely used in gel electrophoresis and Western blotting studies. Products like the GAPDH antibody detect "housekeeping" proteins which are abundantly distributed in cells. This makes them useful for ch...  Read full blog post.

Showing 1-10 of 13 blog posts - Show all blog posts.

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Recent Reviews

4.4
5
7
4
9
3
1
2
0
1
0

Wan-Ching Cheng
09/13/2018
Application: WB
Species: cat

Anonymous
06/29/2017
Application: WB
Species: Mouse and Human

Jim Hsiao
05/22/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol GAPDH
Entrez
Uniprot