GAPDH Antibody (1D4)

Analysis of GAPDH in human nucleus pulposus cell culture (40ug/lane) using anti-GAPDH antibody. LI-COR Odyssey imaging system was used for detection. Western blot image submitted by a verified customer review.

Analysis of of HeLa cells stained with GAPDH antibody, dilution 1:100 (Green). DAPI staining of nuclear DNA (Blue). The GAPDH antibody produces strong cytoplasmic staining of healthy cells.

Analysis of cell line lysates probed with mouse mAb to GAPDH (theoretical molecular weight: 36 kDa), NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.

Human eye cell lysate. Antibody used at 1:500. WB image submitted by a verified customer review.

Analysis of GAPDH in chicken pectoralis major using anti-GAPDH antibody. The primary antibody was used at a dilution of 1:4000, incubated for 1 hour at room temperature. Western blot image submitted by a verified customer review.

Application of the approach to other cell lines. The remainder of the protocol from Figure 2 was then applied using 100 ug/mL digitonin as the optimal cytosolic extract in each case. An aliquot of each extract was then analyzed by 4-12% SDS PAGE followed by Western blotting and probing with antibodies to GAPDH, BiP and Histone H3 (Used as an alternative nuclear marker due to weakness of the Lamin A signal in HeLa cells). Image collected and cropped by CiteAb from the following publication (//bmcresnotes.biomedcentral.com/articles/10.1186/1756-0500-2-243) licensed under a CC-BY license.

Endothelial responses provoked by Hb-lipid interactions are attenuated by sulfide donors. Confluence HAoECs were exposed to the obtained Hb samples for 8 hours and VCAM-1 expression was determined by Western blotting. GAPDH were used as a loading control. Representative experiment, n = 3. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/omcl/2018/3812568/), licensed under a CC-BY license.

Regulation of cell migration-associated proteins by NCKU-21 in A549 and CL1-5 cells. Protein expressions of matrix metalloproteinase-2 (MMP-2) and MMP-9 were analyzed in A549 cell treated with NCKU-21 for 24 hr. * P < 0.05 and ** P < 0.01, compared to the control group (without NCKU-21 treatment). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0185021), licensed under a CC-BY license.

FerrylHb induces HO-1 and ferritin in EC. Confluent HUVECs grown on 6-well plates were exposed to heme, Hb, metHb, and ferrylHb (100 umol/L heme or as indicated in complete medium containing 15% of FBS). After 4 hours of incubation total RNA was isolated and HO-1 mRNA level was measured by quantitative RT-PCR. For protein expression, HUVECs were solubilized after 8 hours of treatment. HO-1 and ferritin H and L expression was detected by Western blot. Immunoblots were reprobed with GAPDH and are representative of three independent experiments. Results are shown as mean +/- S.D. (n = 3) from one representative experiment of three. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/omcl/2013/676425/), licensed under a CC-BY license.

SH-SY5Y cells stained with GAPDH antibody NB300-221 (green). Nuclear DNA is stained with Hoechst dye (blue).

Lane view shows a specific band for GAPDH using 0.05 mg/mL Jurkat and MCF-7 cell lysates and antibody at 1:25. Electropherogram image of corresponding Simple Western lanes view at WES molecular weight of 40 kDa. Image reported by internal validation.

• Reactivity: Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Dr, Eq, Fe, Ha, Ma-Op, Pm, Rb, Sh, Ze
• Applications: WB, Simple Western, ICC/IF, IHC, IP, ChIP, KD, Single-Cell Western
• Clone: 1D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

GAPDH Antibody (1D4) Summary

• Description: This GAPDH antibody (1D4) is primarily used as a loading control in western blots due to the near global expression of GAPDH which can be attributed to its vital role in the glycolytic metabolic process.
• Immunogen: This GAPDH antibody was developed against full length purified porcine GAPDH [UniProt# P00355]
• Marker: Cytosolic Marker
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: GAPDH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Knockdown Validated
  • Simple Western 1:25
  • Single Cell Western
  • Western Blot 1:1000
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  GAPDH Overexpression Lysate

Reactivity Notes

Likely to react with other mammals as well. Hamster, zebrafish and feline reactivity reported by customer reviews. Chicken, rabbit, and Chinese hamster reactivity reported in scientific literature (PMID: 24725420, 17545481, and 24928958 respectively). Drosophila reactivity reported in scientific literature (PMID: 32878003)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% glycerol
• Preservative: 5mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GAPDH Antibody (1D4)

• 1D4 GAPDH
• aging-associated gene 9 protein
• Cytoplasm Marker
• EC 1.2.1
• EC 1.2.1.12
• EC 2.6.99.-
• G3PD antibody
• G3PD
• G3PDH
• GAPD
• GAPDH
• GAPDPeptidyl-cysteine S-nitrosylase GAPDH antibody
• glyceraldehyde 3-phosphate dehydrogenase
• glyceraldehyde-3-phosphate dehydrogenase
• MGC102544
• MGC102546
• MGC103190
• MGC103191
• MGC105239
• MGC127711
• MGC88685
• OCAS, p38
• OCT1 coactivator in S phase
• Peptidyl-cysteine S-nitrosylase GAPDH

Background

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a ubiquitous enzyme involved in glycolysis, converting glyceraldehyde-3-phosphate into 1,3 diphosphoglycerate. This constitutively expressed, homotetramer protein can be found in the nucleus and cytoplasm, and the monomer has a theoretical molecular weight of 36 kDa. Known as a housekeeping gene, GAPDH routinely serves as a control for real-time PCR (RT-PCR) and as a loading control for Western Blots (1-3). In addition to its role in glycolysis, GAPDH has multiple cellular functions including membrane trafficking, transcription activation, apoptosis, DNA repair, and has been implicated in various pathologies such as cancer and neurodegenerative diseases including Alzheimer's and Huntington's (4,5).

References

1) Barber RD, Harmer DW, Coleman RA, Clark BJ. (2005) GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues. Physiol Genomics. 21(3):389-95. PMID: 15769908

2) Jia Y, Takimoto K. (2006) Mitogen-activated protein kinases control cardiac KChIP2 gene expression. Circ Res. 98(3):386-93. PMID: 16385079

3) Godsel LM, Hsieh SN, Amargo EV, Bass AE, Pascoe-McGillicuddy LT, Huen AC, Thorne ME, Gaudry CA, Park JK, Myung K, Goldman RD, Chew TL, Green KJ. (2005) Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. J Cell Biol. 171(6):1045-59. PMID: 16365169

4) Sirover MA1. (1999) New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase. Biochim Biophys Acta. 1432(2): 159-84. PMID: 10407139

5) Tristan C, Shahani N, Sedlak TW, Sawa A. (2011) The diverse functions of GAPDH: views from different subcellular compartments. Cell Signal. 23(2):317-23. PMID: 20727968

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GAPDH Antibody (NB300-221)(400)

Publications using NB300-221

• Huang CL, Chen DY, Tzang CC et al. Celastrol attenuates human parvovirus B19 NS1?induced NLRP3 inflammasome activation in macrophages Molecular Medicine Reports 2023-08-31 [PMID: 37654202] (WB)
• Cheng W, Lawson C, Liu H et al. Exploration of Mediators Associated with Myocardial Remodelling in Feline Hypertrophic Cardiomyopathy Animals 2023-06-26 [PMID: 37443910] (WB, Feline)
• Zhong L. microRNAs mediating E-selectin-dependent metastatic abilities of colon cancer cells and the signaling mechanisms behind their regulation. Thesis 1905-07-10 (WB, Human)
• Arumugam TV, Alli-Shaik A, Liehn EA et al. Multiomics analyses reveal dynamic bioenergetic pathways and functional remodeling of the heart during intermittent fasting Elife 2023-09-28 [PMID: 37769126]
• Kong MJ, Han SJ, Seu SY et al. Shortening of primary cilia length is associated with urine concentration in the kidneys Kidney Research and Clinical Practice 2023-05-31 [PMID: 37313611] (WB)
• Natsagdorj A, Izumi K, Hiratsuka K et al. The CCL2-CCR2 Axis Contributes to Migration of Cabazitaxel-resistant Prostate Cancer Cells Anticancer research 2023-06-01 [PMID: 37247914]
• Abe T, Sato T, Murotomi K Sudachitin and Nobiletin Stimulate Lipolysis via Activation of the cAMP/PKA/HSL Pathway in 3T3-L1 Adipocytes Foods (Basel, Switzerland) 2023-05-10 [PMID: 37238764] (WB, Mouse)
• Wen YC, Tram VTN, Chen WH et al. CHRM4/AKT/MYCN upregulates interferon alpha-17 in the tumor microenvironment to promote neuroendocrine differentiation of prostate cancer Cell death & disease 2023-05-04 [PMID: 37142586] (ChIP, Human)
• Jankauskas SS, Mone P, Avvisato R et al. miR-181c targets Parkin and SMAD7 in human cardiac fibroblasts: Validation of differential microRNA expression in patients with diabetes and heart failure with preserved ejection fraction Mechanisms of ageing and development 2023-04-26 [PMID: 37116731]
• Park YR, Kong MJ, Noh MR, Park KM Rac1 inhibition protects the kidney against kidney ischemia/reperfusion through the inhibition of macrophage migration The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2023-05-01 [PMID: 37078299] (WB, Mouse)
• Azman MS, Alard EL, Dodel M et al. An ERK1/2-driven RNA-binding switch in nucleolin drives ribosome biogenesis and pancreatic tumorigenesis downstream of RAS oncogene The EMBO journal 2023-04-11 [PMID: 37039106] (WB, Mouse)
• Wang H, Poe A, Martinez Yus M et al. Lysyl oxidase-like 2 processing by factor Xa modulates its activity and substrate preference Communications biology 2023-04-07 [PMID: 37029269] (WB)
• Yeung CC, Olesen AT, Wilson R et al. Proteome profiles of intramuscular connective tissue: influence of aging and physical training Journal of applied physiology (Bethesda, Md. : 1985) 2023-05-01 [PMID: 36995911]
• Bonham C, Mandati V, Singh R et al. Coupling substrate-trapping with proximity-labeling to identify protein tyrosine phosphatase PTP1B signaling networks Journal of Biological Chemistry 2023-03-01 [PMID: 36871762]
• Jankauskas SS, Kansakar U, Sardu C et al. COVID-19 Causes Ferroptosis and Oxidative Stress in Human Endothelial Cells Antioxidants (Basel, Switzerland) 2023-01-31 [PMID: 36829885] (WB, Human)
• Aggarwal M. 2,2-Diphenethyl Isothiocyanate Enhances Topoisomerase Inhibitor-Induced Cell Death and Suppresses Multi-Drug Resistance 1 in Breast Cancer Cells Cancers (Basel) 2023-02-01 [PMID: 36765888]
• Kim EJ, Yoon JU, Kim CH et al. Lidocaine inhibits osteogenic differentiation of human dental pulp stem cells in vitro The Journal of international medical research 2023-02-01 [PMID: 36748349] (WB, Human)
• Yamamoto Y, Kurokawa M, Ogawa T et al. Involvement of gap junctions in the increased contraction of bovine oviducts at pre-ovulation observed in vitro Reproduction (Cambridge, England) 2023-03-01 [PMID: 36598933]
• Kalkan H, Pagano E, Paris D et al. Targeting gut dysbiosis against inflammation and impaired autophagy in Duchenne muscular dystrophy EMBO Molecular Medicine 2023-03-08 [PMID: 36594243] (WB, B/N)
• Dufour D, Dumontet T, Sahut-Barnola I et al. Loss of SUMO-specific protease 2 causes isolated glucocorticoid deficiency by blocking adrenal cortex zonal transdifferentiation Research Square 2022-03-18 [PMID: 36543805] (WB, Mouse)
• Abe T Timing of Medium-Chain Triglyceride Consumption Modulates Effects in Mice with Obesity Induced by a High-Fat High-Sucrose Diet Nutrients 2022-12-01 [PMID: 36501131] (WB, Mouse)
• Abe T Timing of Medium-Chain Triglyceride Consumption Modulates Effects in Mice with Obesity Induced by a High-Fat High-Sucrose Diet Nutrients 2022-12-01 [PMID: 36501131] (WB, Mouse)
• Masala N, Pofahl M, Haubrich AN et al. Targeting aberrant dendritic integration to treat cognitive comorbidities of epilepsy Brain 2023-06-01 [PMID: 36448426] (B/N, WB)
• Majumdar U, Choudhury TZ, Manivannan S et al. Single-cell RNA-sequencing analysis of aortic valve interstitial cells demonstrates the regulation of integrin signaling by nitric oxide Frontiers in Cardiovascular Medicine 2022-10-25 [PMID: 36386365] (WB)
• Wang YC, Tsai MC, Chen YS et al. NPRL2 down-regulation facilitates the growth of hepatocellular carcinoma via the mTOR pathway and autophagy suppression Hepatology communications 2022-12-01 [PMID: 36321403] (WB, Human)
• Griem-Krey N, Gauger SJ, Gowing EK et al. The CaMKIIa hub ligand Ph-HTBA promotes neuroprotection after focal ischemic stroke by a distinct molecular interaction Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2022-10-20 [PMID: 36274464] (WB, Mouse)
• Gerwinn T, Salemi S, Schori LJ et al. Improved contractile potential in detrusor microtissues from pediatric patients with end stage lower urinary tract dysfunction Frontiers in Cell and Developmental Biology 2022-10-04 [PMID: 36268506] (ICC/IF)
• Reuter SP, Soonpaa MH, Field D et al. Cardiac Troponin I-interacting Kinase Impacts Cardiomyocyte S-phase Activity But Not Cardiomyocyte Proliferation Circulation 2022-11-16 [PMID: 36382596]
• Kansakar U, Gambardella J, Varzideh F et al. miR-142 Targets TIM-1 in Human Endothelial Cells: Potential Implications for Stroke, COVID-19, Zika, Ebola, Dengue, and Other Viral Infections International Journal of Molecular Sciences 2022-09-06 [PMID: 36142146] (WB)
• Carew NT, Schmidt HM, Yuan S et al. Loss of cardiomyocyte CYB5R3 impairs redox equilibrium and causes sudden cardiac death Journal of Clinical Investigation 2022-09-15 [PMID: 36106636]
• Bousgouni V, Inge O, Robertson D et al. ARHGEF9 regulates melanoma morphogenesis in environments with diverse geometry and elasticity by promoting filopodial-driven adhesion iScience 2022-08-19 [PMID: 36039362] (WB, Human)
  
  Details:
  Dilution used 1:2000
• Ross BD, Jang Y, Welton A et al. A lymphatic-absorbed multi-targeted kinase inhibitor for myelofibrosis therapy Nature communications 2022-08-17 [PMID: 35977945] (WB, Mouse)
• Wavelet-Vermuse C, Odnokoz O, Xue Y et al. CDC20-Mediated hnRNPU Ubiquitination Regulates Chromatin Condensation and Anti-Cancer Drug Response Cancers 2022-07-31 [PMID: 35954396] (WB, Human)
  
  Details:
  Dilution used 1:1000
• Kubota S, Yamamoto Y, Kimura K A Chinese Medicine, Tokishakuyakusan, Increases Bovine Oviductal Tonus via G Protein-Coupled Estrogen Receptor 1 Biological & pharmaceutical bulletin 2022-07-31 [PMID: 35908895] (WB, Bovine)
• Ojeda-Juarez D, Lawrence J, Soldau K et al. Prions induce an early Arc response and a subsequent reduction in mGluR5 in the hippocampus Neurobiology of disease 2022-07-26 [PMID: 35905927] (WB, Mouse)
  
  Details:
  Dilutions: 1:1000
• Jiao H, Wachsmuth L, Wolf S et al. ADAR1 averts fatal type I interferon induction by ZBP1 Nature 2022-07-01 [PMID: 35859176] (WB, Mouse)
• Sugino Y, Sato T, Yamamoto Y, Kimura K. Evaluation of bovine uterine gland functions in 2D and 3D culture system Journal of Reproduction and Development 2022-08-01 [PMID: 35644574] (B/N, WB)
• Kondylis V, Schneider F, Schorn F et al. p62 Promotes Survival and Hepatocarcinogenesis in Mice with Liver-Specific NEMO Ablation Cancers 2022-05-15 [PMID: 35626041] (WB, Mouse)
• Mercado-Ay�n E, Warren N, Halawani S et al. Cerebellar Pathology in an Inducible Mouse Model of Friedreich Ataxia Frontiers in Neuroscience 2022-03-24 [PMID: 35401081] (WB, IHC)
• Huda N, Khambu B, Liu G et al. Senescence Connects Autophagy Deficiency to Inflammation and Tumor Progression in the Liver Cellular and molecular gastroenterology and hepatology 2022-04-08 [PMID: 35398596] (WB, Mouse)
• Stephenson SEM, Costain G, Blok LER et al. Germline variants in tumor suppressor FBXW7 lead to impaired ubiquitination and a neurodevelopmental syndrome American journal of human genetics 2022-04-07 [PMID: 35395208]
• Wen YC, Chen WY, Tram VTN et al. Pyruvate kinase L/R links metabolism dysfunction to neuroendocrine differentiation of prostate cancer by ZBTB10 deficiency Cell death & disease 2022-03-19 [PMID: 35306527] (Chemotaxis, Human)
• Fan CS, Chen CC, Chen LL et al. Extracellular HSP90 alpha Induces MyD88-IRAK Complex-Associated IKK alpha/beta NF-kappa B/IRF3 and JAK2/TYK2-STAT-3 Signaling in Macrophages for Tumor-Promoting M2-Polarization Cells 2022-01-11 [PMID: 35053345] (WB, Human)
• Faucher-Giguere L, Roy A, Deschamps-Francoeur G et al. High-grade ovarian cancer associated H/ACA snoRNAs promote cancer cell proliferation and survival NAR cancer 2022-03-01 [PMID: 35047824] (WB, Human)
• Bennett JS, Gordon DM, Majumdar U et al. Use of machine learning to classify high-risk variants of uncertain significance in lamin A/C cardiac disease Heart rhythm 2021-12-24 [PMID: 34958940]
• Weber RE, Schulze KM, Colburn TD et al. Capillary hemodynamics and contracting skeletal muscle oxygen pressures in male rats with heart failure: Impact of soluble guanylyl cyclase activator Nitric oxide : biology and chemistry 2021-12-04 [PMID: 34871799] (WB, Rat)
• Gerwinn T, Salemi S, Krattiger L Et al. Spheroids of Bladder Smooth Muscle Cells for Bladder Tissue Engineering BioMed research international 2021-11-11 [PMID: 34805410] (WB, Rat)
• Truong Quang BA, Peters R, Cassani DAD Et al. Extent of myosin penetration within the actin cortex regulates cell surface mechanics Nature communications 2021-11-11 [PMID: 34764258] (WB, Human)
• Heath J, Cheyou ES, Findlay S Et al. POGZ promotes homology-directed DNA repair in an HP1-dependent manner EMBO reports 2021-11-10 [PMID: 34758190]
• Zhao W, Li X, Nian W et al. Ribosome Proteins Represented by RPL27A Mark the Development and Metastasis of Triple-Negative Breast Cancer in Mouse and Human Frontiers in Cell and Developmental Biology 2021-08-23 [PMID: 34497807] (WB, B/N)
• Muzorewa TT, Buerk DG, Jaron D, Barbee KA Coordinated regulation of endothelial calcium signaling and shear stress-induced nitric oxide production by PKC beta and PKC eta. Cellular signalling 2021-08-30 [PMID: 34474112] (WB, Bovine)
• Javeed N, Her TK, Brown MR Et al. Pro-inflammatory beta cell small extracellular vesicles induce beta cell failure through activation of the CXCL10/CXCR3 axis in diabetes Cell reports 2021-08-24 [PMID: 34433033] (WB, Mouse)
• Lin HW, Lee JY, Chou NL Et al. Phosphorylation of PUF-A/PUM3 on Y259 modulates PUF-A stability and cell proliferation PloS one 2021-08-18 [PMID: 34407138]
• Wang H, Chen J, Jandu S Et al. Probing tissue transglutaminase mediated vascular smooth muscle cell aging using a novel transamidation-deficient Tgm2-C277S mouse model Cell death discovery 2021-07-29 [PMID: 34326316] (WB)
• Jo H, Park H, Choi Y et al. Portal Modulation Effects of Terlipressin on Liver Regeneration and Survival in a Porcine Model Subjected to 90% Hepatectomy Am J Transl Res 2021-07-26 [PMID: 34306332]
• Pan CH, Lin YP, Wang JY et al. Preventive Effect and Mechanism of Crossostephium chinense Extract on Balloon Angioplasty-Induced Neointimal Hyperplasia Evidence-based complementary and alternative medicine : eCAM 2021-06-30 [PMID: 34306155] (WB)
• Behere A, Thornqvist Po, Winberg S Et Al. Visualization of early oligomeric alpha-synuclein pathology and its impact on the dopaminergic system in the (Thy-1)-h[A30P]alpha-syn transgenic mouse model Journal of neuroscience research 2021-07-22 [PMID: 34292621] (WB)
• Zaganjor E, Yoon H, Spinelli JB et al. SIRT4 is an early regulator of branched-chain amino acid catabolism that promotes adipogenesis Cell reports 2021-07-13 [PMID: 34260923] (WB)
• Wang H, Poe A, Pak L Et Al. An in situ activity assay for lysyl oxidases Communications biology 2021-07-05 [PMID: 34226627]
• Bachmann S, Bell M, Klimek J, Zempel H. Differential Effects of the Six Human TAU Isoforms: Somatic Retention of 2N-TAU and Increased Microtubule Number Induced by 4R-TAU Frontiers in Neuroscience 2021-05-25 [PMID: 34113229] (ICC/IF)
• Hsieh Y, Lee K, Lei H et al. (Pro)renin Receptor Knockdown Attenuates Liver Fibrosis through Inactivation of ERK/TGF-beta1/SMAD3 Pathway Cellular and Molecular Gastroenterology and Hepatology 2021-06-01 [PMID: 34087453]
• Millan C, Prause L, Vallmajo-Martin Q et al. Extracellular Vesicles from 3D Engineered Microtissues Harbor Disease-Related Cargo Absent in EVs from 2D Cultures Advanced healthcare materials 2021-04-22 [PMID: 33890421]
• Guldiken N, Argemi J, Gurbuz B et al. Serum transferrin as a biomarker of hepatocyte nuclear factor 4 alpha activity and hepatocyte function in liver diseases BMC medicine 2021-02-17 [PMID: 33593348]
• Ryder BD, Matlahov I, Bali S et al. Regulatory inter-domain interactions influence Hsp70 recruitment to the DnaJB8 chaperone Nature communications 2021-02-11 [PMID: 33574241] (WB, Human)
• Majumdar U, Manivannan S, Basu M et al. Nitric oxide prevents aortic valve calcification by S-nitrosylation of USP9X to activate NOTCH signaling Science advances 2021-02-01 [PMID: 33547080] (WB, Human)
• Liu T, Xing Y, Fan X et al. Fasting and overfeeding affect the expression of the immunity- or inflammation-related genes in the liver of poultry via endogenous retrovirus (ERV) Poult Sci 2021-02-01 [PMID: 33518151]
• Makino T, Izumi K, Hiratsuka K, et al. Anti-proliferative and anti-migratory properties of coffee diterpenes kahweol acetate and cafestol in human renal cancer cells Scientific reports 2021-01-12 [PMID: 33436830] (Human)
• Fusco P, Mattiuzzo E, Frasson C et al. Verteporfin induces apoptosis and reduces the stem cell-like properties in Neuroblastoma tumour-initiating cells through inhibition of the YAP/TAZ pathway European journal of pharmacology 2020-12-18 [PMID: 33347823] (WB, Human)
• Sanchez-Felipe, L, Vercruysse, T Et al. A single-dose live-attenuated YF17D-vectored SARS-CoV-2 vaccine candidate. Nature 2020-12-01 [PMID: 33260195] (WB, Mouse)
• Schlesinger S, Seo S, Pryce B et al. Loss of Smad4 in the scleraxis cell lineage results in postnatal joint contracture Dev Biol 2020-11-25 [PMID: 33248111]
• Dermit M, Dodel M, Lee FCY et al. Subcellular mRNA Localization Regulates Ribosome Biogenesis in Migrating Cells Dev Cell 2020-11-09 [PMID: 33171110] (WB, Human)
  
  Details:
  Used as a control in Western blot along with HEK293T LARP6-knockout cells.
• Pan C, Chen S, Wang J et al. Sclareol ameliorated ERCC1-mediated cisplatin resistance in A549 human lung adenocarcinoma cells and a murine xenograft tumor model by suppressing AKT-GSK3 beta-AP1/Snail and JNK-AP1 pathways Chemico-Biological Interactions 2020-10-01 [PMID: 33164868] (WB)
• Yan S, Khambu B, Chen X et al. Hepatic autophagy deficiency remodels gut microbiota for an adaptive protection via FGF15-FGFR4 signaling Cell Mol Gastroenterol Hepatol 2020-10-22 [PMID: 33127558] (WB, Mouse)
• Bahmad HF, Chalhoub RM, Harati H et al. Tideglusib attenuates growth of neuroblastoma cancer stem/progenitor cells in vitro and in vivo by specifically targeting GSK-3 beta Pharmacol Rep 2020-10-08 [PMID: 33030673]
• Macke A, Lopez W, Carlson DJ, Carlson KA Nora Virus VP4b and ORF1 Circulate in Hemolymph of Infected D. melanogaster with Coordinate Expression of Vago and Vir-1 Vaccines 2020-08-31 [PMID: 32878003] (WB, Drosophila)
• Chunchi Y, Zhao M, Li S Et Al. Increase of E3 ubiquitin-ligase NEDD4 expression leads to degradation of its target proteins PTEN/IGF1R during the formation of goose fatty liver J. Anim. Sci. 2020-08-25 [PMID: 32841331]
• Zhao S, Yuan C, Tuo X et al. MCLR induces dysregulation of calcium homeostasis and endoplasmic reticulum stress resulting in apoptosis in Sertoli cells Chemosphere 2020-08-15 [PMID: 32828052]
• Gordon SM, Nishiguchi MA, Mani S, Mainigi MA Interferons drive development of novel interleukin-15-responsive macrophages in the uterus J Immunol 2020-07-22 [PMID: 32690654]
• Potor L, Eva SK, Hegedus H et al. The Fungal Iron Chelator Desferricoprogen Inhibits Atherosclerotic Plaque Formation Int J Mol Sci 2020-07-03 [PMID: 32635347] (WB, Human)
• Wang Y, Ji N, Gong X et al. Thioredoxin-1 attenuates atherosclerosis development through inhibiting NLRP3 inflammasome Endocrine 2020-06-30 [PMID: 32607763] (WB)
• Xiong L, Zhao K, Cao Y et al. Linking skeletal muscle aging with osteoporosis by lamin A/C deficiency PLoS Biol. 2020-06-01 [PMID: 32479501] (WB, Mouse)
• Chen KC, Chen CR, Chen CY et al. Bicalutamide Elicits Renal Damage by Causing Mitochondrial Dysfunction via ROS Damage and Upregulation of HIF-1 Int J Mol Sci 2020-05-11 [PMID: 32403414] (WB, Rat)
• Schwarzer R, Jiao H, Wachsmuth L et al. FADD and Caspase-8 Regulate Gut Homeostasis and Inflammation by Controlling MLKL- and GSDMD-Mediated Death of Intestinal Epithelial Cells Immunity 2020-04-22 [PMID: 32362323]
• Jiao H, Wachsmuth L, Kumari S et Al. Z-nucleic-acid sensing triggers ZBP1-dependent necroptosis and inflammation Nature 2020-03-25 [PMID: 32296175] (WB, Mouse)
• Tu PS, Lin EC, Chen HW et al. The extracellular signal-regulated kinase 1/2 modulates the intracellular localization of DNA methyltransferase 3A to regulate erythrocytic differentiation Am J Transl Res 2020-03-15 [PMID: 32269731] (WB, Mouse, Human)
• Laurien L, Nagata M, SchUnke H et al. Autophosphorylation at serine 166 regulates RIP kinase 1-mediated cell death and inflammation Nat Commun 2020-04-08 [PMID: 32269263] (IP, Mouse)
• Jannuzzi A, Arslan S, Yilmaz A et Al. Higher proteotoxic stress rather than mitochondrial damage is involved in higher neurotoxicity of bortezomib compared to carfilzomib Redox Biology 2020-01-01 [PMID: 32244176] (WB, PCR, Human)
• Zavaczki E, Gall T, Zarjou A et Al. Ferryl Hemoglobin Inhibits Osteoclastic Differentiation of Macrophages in Hemorrhaged Atherosclerotic Plaques Oxid Med Cell Longev 2020-02-27 [PMID: 32184915] (WB, Mouse)
• Morelli Ae, Sumpter Tl, Rojas-Canales Dm et Al. Neurokinin-1 Receptor Signaling Is Required for Efficient Ca2+ Flux in T-Cell-Receptor-Activated T Cells Cell Rep 2020-03-10 [PMID: 32160549] (WB, Mouse)
• Cipollari, E, Szapary, H J Et al. Correlates and Predictors of Cerebrospinal Fluid Cholesterol Efflux Capacity from Neural Cells, a Family of Biomarkers for Cholesterol Epidemiology in Alzheimer's Disease. J Alzheimers Dis 2020-02-18 [PMID: 32065798] (WB, Rat)
• Posta N, Csosz E, Oros M et al. Hemoglobin oxidation generates globin-derived peptides in atherosclerotic lesions and intraventricular hemorrhage of the brain, provoking endothelial dysfunction Lab. Invest. 2020-02-13 [PMID: 32054994] (WB, Human)
• Davis DL, Mahawar U, Pope VS, Allegood J Dynamics of sphingolipids and the serine-palmitoyltransferase complex in oligodendrocytes during myelination. J Lipid Res 2020-02-12 [PMID: 32041816]
• Grolla AA, Miggiano R, Di Marino D et al. A nicotinamide phosphoribosyltransferase-GAPDH interaction sustains the stress-induced NMN/NAD+ salvage pathway in the nucleus J. Biol. Chem. 2020-01-27 [PMID: 31988240] (WB, ICC/IF, Mouse, Human)
• Kadomoto S, Izumi K, Hiratsuka K et al. Tumor-Associated Macrophages Induce Migration of Renal Cell Carcinoma Cells via Activation of the CCL20-CCR6 Axis Cancers (Basel) 2019-12-30 [PMID: 31905918]
• Zerfas B, Trader D Synthesis and Application of an Activity Based Peptide Peptoid Hybrid Probe for the Immunoproteasome Current Protocols in Chemical Biology 2019-12-01 [PMID: 31860787] (WB, Human)
• Msheik H, El Hayek S, Bari MF, et al. Transcriptomic profiling of trophoblast fusion using BeWo and JEG-3 cell lines Mol. Hum. Reprod. 2019-12-01 [PMID: 31778538] (WB, Human)
• Apaoblaza A, Gerrard SD, Matarneh SK et al. Muscle from grass- and grain-fed cattle differs energetically Meat Sci. 2019-11-06 [PMID: 31734468] (WB, Bovine)
• Zhang H, Gong X, Ni S et al. C1q/TNF-related protein-9 attenuates atherosclerosis through AMPK-NLRP3 inflammasome singling pathway Int. Immunopharmacol. 2019-11-11 [PMID: 31727560] (WB, Mouse)
• Choi H, Madhu V, Shapiro IM, Risbud MV Nucleus pulposus primary cilia alter their length in response to changes in extracellular osmolarity but do not control TonEBP-mediated osmoregulation Sci Rep 2019-10-29 [PMID: 31664118] (WB, Human)
• Wang Y, Cao J, Liu W et al. Protein tyrosine phosphatase receptor type R (PTPRR) antagonizes the Wnt signaling pathway in ovarian cancer by dephosphorylating and inactivating beta-catenin J. Biol. Chem. 2019-10-25 [PMID: 31653698] (WB, IP, Human)
• Fusco P, Parisatto B, Rampazzo E et al. Patient-derived organoids (PDOs) as a novel in vitro model for neuroblastoma tumours BMC Cancer 2019-10-21 [PMID: 31638925] (WB, Human)
• Matthaeus C, Lian X et al. eNOS-NO-induced small blood vessel relaxation requires EHD2-dependent caveolae stabilization. PLoS One 2019-11-10 [PMID: 31600286] (WB, Mouse)
• Deora V, Lee JD, Albornoz EA et al. The microglial NLRP3 inflammasome is activated by amyotrophic lateral sclerosis proteins Glia 2019-10-09 [PMID: 31596526]
• Kovalchuke L, Mosharov EV, Levy OA, Greene LA Stress-induced phospho-ubiquitin formation causes parkin degradation Sci Rep 2019-08-12 [PMID: 31406131] (WB, Rat)
• Gunasekar P, Satish M, Dabestani P et al. Modulation of Cathepsin L Expression in the Coronary Arteries of Atherosclerotic Swine J Surg Res 2019-08-05 [PMID: 31377485] (WB, Porcine)
• Crawley JTB, Zalli A, Monkman JH et al. Defective fibrin deposition and thrombus stability in Bambi-/- mice is mediated by elevated anticoagulant function J. Thromb. Haemost. 2019-07-27 [PMID: 31351019] (WB, Mouse)
• Aggarwal M, Saxena R, Asif N et al. p53 mutant-type in human prostate cancer cells determines the sensitivity to phenethyl isothiocyanate induced growth inhibition J. Exp. Clin. Cancer Res. 2019-07-15 [PMID: 31307507] (IP, WB, Human, Mouse)
• Penas C, Maloof ME, Stathias V et al. Time series modeling of cell cycle exit identifies Brd4 dependent regulation of cerebellar neurogenesis Nat Commun 2019-07-10 [PMID: 31292434] (WB, Mouse)
• Epp AL, Ebert SN, Sanchez-Arias JC et al. A novel motif in the proximal C-terminus of Pannexin 1 regulates cell surface localization Sci Rep 2019-07-05 [PMID: 31278290] (WB, Human)
• Yan S, Zhou J, Chen X et al. Diverse Consequences in Liver Injury in Mice with Different Autophagy Functional Status Treated with Alcohol Am. J. Pathol. 2019-06-11 [PMID: 31199920] (Mouse)
• Hans CP, Sharma N, Sen S et al. Transcriptomics Analysis Reveals New Insights into the Roles of Notch1 Signaling on Macrophage Polarization Sci Rep 2019-05-29 [PMID: 31142802] (WB, Mouse)
• Kuo T, Damle M, Gonzalez BJ et al. Mesenchymal Stromal Cells Are Required for Regeneration and Homeostatic Maintenance of Skeletal Muscle Cell Rep 2019-05-14 [PMID: 31091443] (Single Cell Western, Mouse)
• Polykratis A, Martens A, Eren RO et al. A20 prevents inflammasome-dependent arthritis by inhibiting macrophage necroptosis through its ZnF7 ubiquitin-binding domain Nat. Cell Biol. 2019-05-13 [PMID: 31086261] (WB, Mouse)
• Sikura KE, Potor L, Szerafin T, Oros M et al. Hydrogen sulfide abrogates heart valve calcification: Implications for calcific aortic valve disease Br. J. Pharmacol. 2019-04-24 [PMID: 31017307] (WB, Human)
• Zhou, C;Zeldin, Y;Baratz, ME;Kathju, S;Satish, L; Investigating the effects of Pirfenidone on TGF-Beta 1 stimulated non-SMAD signaling pathways in Dupuytren's disease -derived fibroblasts BMC Musculoskelet Disord 2019-03-30 [PMID: 30927912] (WB, Human)
• Ren W, Hou J, Yang C et al. Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery J. Exp. Clin. Cancer Res. 2019-02-08 [PMID: 30736829] (WB, Human)
• Wang CN, Lin YC, Chang BC et al. Targeting the phosphorylation site of MARCKS alleviates symptoms of steroid-resistant like asthma in a murine model Br. J. Pharmacol. 2019-02-01 [PMID: 30706455] (WB, Mouse)
• Iwamoto H, Izumi K, Natsagdorj A et al. Coffee diterpenes kahweol acetate and cafestol synergistically inhibit the proliferation and migration of prostate cancer cells Prostate 2018-12-19 [PMID: 30569541] (WB, Human)
• Natsagdorj A, Izumi K, Hiratsuka K et al. CCL2 induces resistance to the antiproliferative effect of cabazitaxel in prostate cancer cells. Cancer Sci. 2019-01-01 [PMID: 30426599] (WB, Human)
• Qi M, Zhou Y, Liu J et al. AngII induces HepG2 cells to activate epithelial-mesenchymal transition. Exp Ther Med 2018-10-01 [PMID: 30233697] (WB, Human)
• Huang F, Ben Aissa M, Levesque G, Carreau M. FANCC localizes with UNC5A at neurite outgrowth and promotes neuritogenesis. BMC Res Notes 2018-09-12 [PMID: 30213274] (WB, Mouse)
• Tu W, Cheng C, Chen C et al. Annotation of differential protein expression in the hypothalami of layer-type Taiwan country chickens in response to acute heat stress Journal of Thermal Biology 2018-10-01 [PMID: 30196895] (WB, Chicken)
• Rudman M. Bromodomain and Extraterminal Domain-Containing Protein Inhibition Decreases Inflammation after Spinal Cord Injury Exp Neurol 2018-08-23 [PMID: 30134146]
• Tu WL, Cheng CY, Chen CJ et al. Proteomic analysis of the hypothalamus of broiler-type Taiwan country chickens in response to acute heat stress Anim. Sci. J. 2018-08-20 [PMID: 30125421] (WB, Chicken)
• Iannotti FA, Pagano E, Moriello AS et al. Effects of non-euphoric plant cannabinoids on muscle quality and performance of dystrophic mdx mice Br. J. Pharmacol. 2018-08-03 [PMID: 30074247] (WB, Mouse)
• Zhang H, Yan S, khambu B et al. Dynamic MTORC1-TFEB feedback signaling regulates hepatic autophagy, steatosis and liver injury in long-term nutrient oversupply. Autophagy. 2018-07-25 [PMID: 30044707] (WB, Mouse)
• Ter-Avetisyan G, Dumoulin A, Herrel A et al. Loss of Axon Bifurcation in Mesencephalic Trigeminal Neurons Impairs the Maximal Biting Force in Npr2-Deficient Mice Front Cell Neurosci. [PMID: 29962937] (WB, Mouse)
• Chen YH, Chen CJ, Wang SJ et al. Downregulation of tight junction protein zonula occludens-2 and urothelium damage in a cyclophosphamide-induced mouse model of cystitis. Taiwan J Obstet Gynecol 2018-06-01 [PMID: 29880173]
• Zhang Y, Chang JF, Sun J et al. Histone H3K27 methylation is required for NHEJ and genome stability by modulating the dynamics of FANCD2 on chromatin J. Cell. Sci. 2018-05-14 [PMID: 29760279] (WB, Human)
• Davis S, Cirone A M et al. Phagocytosis-mediated M1 activation by chitin but not by chitosan. Am J Physiol Cell Physiol 2018-01-07 [PMID: 29719169] (WB, Mouse)
• Choi H, Chaiyamongkol W, Doolittle AC et al. COX-2 expression mediated by calcium-TonEBP signaling axis under hyperosmotic conditions serves osmoprotective function in nucleus pulposus cells. J Biol Chem 2018-06-08 [PMID: 29700115] (Mouse)
• Chen L, Zhu G, Johns EM, Yang X. TRIM11 activates the proteasome and promotes overall protein degradation by regulating USP14. Nat Commun. 2018-03-26 [PMID: 29581427] (WB, Human)
• Maolake A, Izumi K, Natsagdorj A et al. Tumor necrosis factor-alpha induces prostate cancer cell migration in lymphatic metastasis through CCR7 upregulation Cancer Sci. 2018-05-01 [PMID: 29575464] (WB, Human)
• Potor L, Jeney V, Nagy P et al. Hydrogen Sulfide Abrogates Hemoglobin-Lipid Interaction In Atherosclerotic Lesion. Oxidative Medicine and Cellular Longevity 1905-07-09 [PMID: 29560080] (WB, Human)
• Troster P, Haseleu J, Petersen J, Drees O. The Absence of Sensory Axon Bifurcation Affects Nociception and Termination Fields of Afferents in the Spinal Cord. Front Mol Neurosci. 2018-02-08 [PMID: 29472841] (WB, Mouse)
• Fan CS, Chen WS, Chen LL, Chen CC. Osteopontin-integrin engagement induces HIF-1a-TCF12-mediated endothelial-mesenchymal transition to exacerbate colorectal cancer. Oncotarget. 2018-01-12 [PMID: 29435158] (WB, Human)
• Langhorst H, Juttner R, Groneberg D et al. The IgCAM CLMP is required for intestinal and ureteral smooth muscle contraction by regulating Connexin43 and 45 expression in mice Dis Model Mech 2018-01-19 [PMID: 29361518] (Mouse)
• Urata S, Izumi K, Hiratsuka K et al. C-C motif ligand 5 promotes migration of prostate cancer cells in the prostate cancer bone metastasis microenvironment. Cancer Sci. 2018-03-01 [PMID: 29288523] (WB, Human)
• Fustaino V, Presutti D, Colombo T et al. Characterization of epithelial-mesenchymal transition intermediate/hybrid phenotypes associated to resistance to EGFR inhibitors in non-small cell lung cancer cell lines. Oncotarget 2017-11-28 [PMID: 29262566] (WB, Human)
• Lin H, Magrane J, Clark EM et al. Early VGLUT1-specific parallel fiber synaptic deficits and dysregulated cerebellar circuit in the KIKO mouse model of Friedreich ataxia. Dis Model Mech 2017-12-19 [PMID: 29259026] (WB, Mouse)
• Craighead D H, Wang H et al. Acute lysyl oxidase inhibition alters microvascular function in normotensive but not hypertensive men and women. Am J Physiol Heart Circ Physiol 2018-01-03 [PMID: 29167120] (WB, Human)
• Nguyen TP, Yong HE, Chollangi T et al. Altered downstream target gene expression of the placental Vitamin D receptor in human idiopathic fetal growth restriction Cell Cycle. 2017-11-22 [PMID: 29161966] (WB, Human)
• Liu C, Lee WC, Huang BM et al. 16-Hydroxycleroda-3, 13-dien-15, 16-olide inhibits the proliferation and induces mitochondrial-dependent apoptosis through Akt, mTOR, and MEK-ERK pathways in human renal carcinoma cells. Phytomedicine. 2017-10-03 [PMID: 29157834] (Human)
• Lin H, Magrane J, Rattelle A et al. Early cerebellar deficits in mitochondrial biogenesis and respiratory chain complexes in the KIKO mouse model of Friedreich ataxia Dis Model Mech. 2017-11-01 [PMID: 29125827] (WB, Mouse)
• Zeng Yen-Jing, Lin Yu-Hsiang, Wang You-Cheng et al. Laser acupuncture-induced analgesic effect and molecular alteRations in an incision pain model: a comparison with electroacupuncture-induced effects. Lasers in Medical Science 2018-01-01 [PMID: 29103083] (WB, Rat)
• Rostami J, Holmqvist S, Lindstrom V et al. Human astrocytes transfer aggregated alpha-synuclein via tunneling nanotubes J. Neurosci. 2017-10-31 [PMID: 29089438] (WB, Human)
• Han J, Plummer J, Liu L et al. The impact of obesity on brain iron levels and a-synuclein expression is regionally dependent. Nutr Neurosci. 2017-10-16 [PMID: 29034829] (WB, Mouse)
• Barone V, Lang M et al. An Effective Feedback Loop between Cell-Cell Contact Duration and Morphogen Signaling Determines Cell Fate. Dev Cell 2017-10-23 [PMID: 29033362] (WB, Zebrafish)
• Lin X, Wang L, Wang F Chondromodulin-I suppresses tumorigenesis of human osteosarcoma cells Mol Med Rep 2017-01-01 [PMID: 28983591] (WB, Human)
• Sriwattanapong K, Slocum SL, Chawanthayatham S et al. Editor's Highlight: Pregnancy Alters Aflatoxin B1 Metabolism and Increases DNA Damage in Mouse Liver. Toxicol. Sci. 2017-11-01 [PMID: 28973694] (WB, Mouse)
  
  Details:
  Novus GAPDH antibody clone 1D4 was used as a loading control for western blot to look at metabolic enzyme levels in pregnant vs nonpregnant mice
• Liao HF, Pan CH, Chou PY et al. Toxicological effects of NCKU-21, a phenanthrene derivative, on cell growth and migration of A549 and CL1-5 human lung adenocarcinoma cells PLoS ONE 2017-09-25 [PMID: 28945763] (WB, Human)
• Bove A, Gradeci D, Fujita Y et al. Local cellular neighbourhood controls proliferation in cell competition Mol. Biol. Cell 2017-09-20 [PMID: 28931601] (WB)
• Gillen AE, Brechbuhl HM, Yamamoto TM et al. Alternative Polyadenylation of PRELID1 Regulates Mitochondrial ROS Signaling and Cancer Outcomes Mol. Cancer Res. 2017-09-14 [PMID: 28912168] (WB, Human)
• Martin IV, Bohner A, Boor P et al. Complement C5a receptors C5L2 and C5aR in renal fibrosis Am. J. Physiol. Renal Physiol. 2017-09-13 [PMID: 28903945] (WB, Mouse)
• Billon P, Bryant EE, Joseph SA et al. CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons. Mol. Cell 2017-09-21 [PMID: 28890334] (WB, Human)
• Xu Q, Jitkaew S, Choksi S et al. The cytoplasmic nuclear receptor RARg controls RIP1 initiated cell death when cIAP activity is inhibited Nat Commun 2017-09-04 [PMID: 28871172] (WB, Mouse)
• Bachmann S, Bell M, Klimek J, Zempel H Subcellular localization of TAU isoforms and their influence on microtubule dynamics bioRxiv 2020-06-17
• Lee A, Rayner SL, Gwee SSL et al. Pathogenic mutation in the ALS/FTD gene, CCNF, causes elevated Lys48-linked ubiquitylation and defective autophagy. Cell Mol Life Sci 2018-01-01 [PMID: 28852778] (Human)
• Sun Chongxiu, Simon Scott I, Foster Greg A et al. 11,12-Epoxyecosatrienoic acids mitigate endothelial dysfunction associated with estrogen loss and aging: Role of membrane depolarization. Journal of Molecular and Cellular Cardiology 2016-01-01 [PMID: 27079253] (WB, Human)
• Krapp C, Hotter D, Gawanbacht A et al. Guanylate Binding Protein (GBP) 5 Is an Interferon-Inducible Inhibitor of HIV-1 Infectivity. Cell Host Microbe. 2016-03-15 [PMID: 26996307] (WB, Human)
• Kumper S, Mardakheh FK, McCarthy A et al. Rho-associated kinase (ROCK) function is essential for cell cycle progression, senescence and tumorigenesis. Elife. 2016-01-14 [PMID: 26765561] (WB, Mouse)
  
  Details:
  GAPDH antibody used as a loading control for WB on mouse embryo fibroblasts (MEFs) from embryos with different genotypes (Figure 1F).
• Chen YC, Statt S, Wu R et al. High mobility group box 1-induced epithelial mesenchymal transition in human airway epithelial cells. Sci Rep. 2016-01-07 [PMID: 26739898] (WB, Human)
• Slocum SL, Skoko JJ, Wakabayashi N et al. Keap1/Nrf2 pathway activation leads to a repressed hepatic gluconeogenic and lipogenic program in mice on a high-fat diet. Arch. Biochem. Biophys. 2015-12-14 [PMID: 26701603] (WB, Mouse)
• Brai E, Marathe S, Astori S et al. Notch1 Regulates Hippocampal Plasticity Through Interaction with the Reelin Pathway, Glutamatergic Transmission and CREB Signaling. Front Cell Neurosci. 2015-12-04 [PMID: 26635527] (WB, Mouse)
• Gokhin DS, Ochala J, Domenighetti AA, Fowler VM. Tropomodulin1 directly controls thin filament length in both wild-type and tropomodulin4-deficient skeletal muscle. Development. 2015-11-19 [PMID: 26586224] (WB, Mouse)
• Presutti D, Santini S, Cardinali B et al. MET Gene Amplification and MET Receptor Activation Are Not Sufficient to Predict Efficacy of Combined MET and EGFR Inhibitors in EGFR TKI-Resistant NSCLC Cells. PLoS ONE. 2015-11-20 [PMID: 26580964] (WB, Human)
• Becs G, Zarjou A, Agarwal A et al. Pharmacological induction of ferritin prevents osteoblastic transformation of smooth muscle cells. J Cell Mol Med 2016-02-01 [PMID: 26499096]
• Shang Y, Dong X, Han G et al. The Roles of Tight Junctions and Claudin-1 in the Microbubble-Mediated Ultrasound-Induced Enhancement of Drug Concentrations in Rat Prostate. J. Membr. Biol. 2015-08-20 [PMID: 26289600] (WB, Rat)
• Ikhapoh IA, Pelham CJ, Agrawal DK. Atherogenic Cytokines Regulate VEGF-A-Induced Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells into Endothelial Cells. Stem Cells Int 2015-01-01 [PMID: 26106428] (WB)
• Zakrzewicz A, Atanasova S, Padberg W. Monocytic Tissue Transglutaminase in a Rat Model for Reversible Acute Rejection and Chronic Renal Allograft Injury. Mediators of Inflammation. 2015-01-01 [PMID: 26063971] (WB, Rat)
• Boosani CS, Dhar K, Agrawal DK. Down-regulation of hsa-miR-1264 contributes to DNMT1-mediated silencing of SOCS3. Mol. Biol. Rep. 2015-06-06 [PMID: 26047583] (WB, Human)
  
  Details:
  GAPDH antibody (clone 1D4) used for WB as a loading control on HCASMCs (human coronary artery smooth muscle) cells. Cells were transfected with hsa-miR-1264 mimic or its inhibitor and treated with or without TNF-alpha and IGF-1 (Fig. 3).
• Serbo JV, Kuo S, Lewis S et al. Patterning of Fibroblast and Matrix Anisotropy within 3D Confinement is Driven by the Cytoskeleton Adv Healthc Mater. 2015-06-01 [PMID: 26033825]
• Chelluboina B, Warhekar A, Dillard M et al. Post-transcriptional inactivation of matrix metalloproteinase-12 after focal cerebral ischemia attenuates brain damage. Sci Rep. 2015-05-09 [PMID: 25955565] (WB, Rat)
• Baskar K, Sur S, Selvaraj V, Agrawal DK Functional constituents of a local serotonergic system, intrinsic to the human coronary artery smooth muscle cells. Mol Biol Rep 2015-08-01 [PMID: 25861735] (Human)
• Aboelenain M, Kawahara M, Balboula AZ et al. Status of autophagy, lysosome activity and apoptosis during corpus luteum regression in cattle. J Reprod Dev 2015-06-20 [PMID: 25819401] (WB, Bovine)
• Liu YN, Yin J, Barrett B et al. Loss of Androgen Regulated miR-1 Activates SRC and Promotes Prostate Cancer Bone Metastasis Mol. Cell. Biol. 2015-03-23 [PMID: 25802280] (Chemotaxis, WB, Mouse)
• De Boussac H, Maqdasy S, Trousson A et al. Enolase is regulated by Liver X Receptors Steroids. 2015-02-21 [PMID: 25708389] (WB, Mouse)
• Kutz JL, Greaney JL, Santhanam L, Alexander LM. Evidence for a functional vasodilatory role for hydrogen sulfide in the human cutaneous microvasculature J. Physiol. (Lond.). 2015-01-30 [PMID: 25639684] (WB, Human, Rat)
• Li J, Yuan W, Jiang S et al. Prolyl-4-hydroxylase Domain Protein 2 Controls NF-kB/p65 Transactivation and Enhances the Catabolic Effects of Inflammatory Cytokines on cells of the Nucleus Pulposus J. Biol. Chem. 2015-01-29 [PMID: 25635047] (WB, Human, Rat)
• Izuagie IA, Pelham CJ, Agrawal DK. Synergistic effect of angiotensin II on vascular endothelial growth factor-A-mediated differentiation of bone marrow-derived mesenchymal stem cells into endothelial cells. Stem Cell Res Ther 2015-01-06 [PMID: 25563650]
• Ye W, Zhou J, Markova DZ et al. Xylosyltransferase-1 Expression Is Refractory to Inhibition by the Inflammatory Cytokines Tumor Necrosis Factor alpha and IL-1b in Nucleus Pulposus Cells: Novel Regulation by AP-1, Sp1, and Sp3. Am. J. Pathol. 2014-12-02 [PMID: 25476526] (WB, Human)
  
  Details:
  GAPDH antibody used for WB on lysates of human nucleus pulposus (NP) cells transduced with LV-shC or LV-shSp1 or LV-shSp3 (Figure 6)
• Liu Jt, Bain Lj. Arsenic inhibits hedgehog signaling during P19 cell differentiation. Toxicol. Appl. Pharmacol. 2014-12-15 [PMID: 25448440] (WB, Mouse)
  
  Details:
  GAPDH antibody used for WB at 1:1000 dilution on lysates of mouse embryonal carcinoma P19 cell line which was induced to differentiate for 5 days while being exposed to 0, 0.25, or 0.5 uM arsenic/ sodium arsenite (Fig 3A and 3C; Fig 6).
• Chang Sh, Chang Wl, Lu Cc, Tarn Wy. Alanine repeats influence protein localization in splicing speckles and paraspeckles. Nucleic Acids Res. 2014-11-20 [PMID: 25414336]
• Zhao N, Koenig Sn, Trask Aj et al. mir145 Regulates TGFBR2 Expression and Matrix Synthesis in Vascular Smooth Muscle Cells. Circ. Res. 2014-10-16 [PMID: 25323858] (WB, Mouse)
• Peterson Jm, Mart R, Bond Ce. Effect of obesity and exercise on the expression of the novel myokines, Myonectin and Fibronectin type III domain containing 5. PeerJ. 2014-10-07 [PMID: 25289190] (WB, Rat)
• Domingue JC, Ao M, Sarathy J et al. HEK-293 cells expressing the cystic fibrosis transmembrane conductance regulator (CFTR): a model for studying regulation of Cl- transport. Physiol Rep. 2014-09-01 [PMID: 25263207] (WB, Human)
  
  Details:
  MRP4 antibody used for WB at 1:750 dilution on cytosolic and total lysate fractions of HEK?CFTR cells as well as T84 cells (positive control). The antibody detected a band of 170 kDa size and the data is shown in Figure 5A - lower panel.
• Chen X, Sadowska GB, Zhang et al. Neutralizing anti-interleukin-1b antibodies modulate fetal blood-brain barrier function after ischemia. Neurobiol. Dis. 2014-09-24 [PMID: 25258170] (WB, Sheep)
• Wang X, Wang H, Yang H et al. Tumor Necrosis Factor-a- and Interleukin-1b-Dependent Matrix Metalloproteinase-3 Expression in Nucleus Pulposus Cells Requires Cooperative Signaling via Syndecan 4 and Mitogen-Activated Protein Kinase-Nuclear Factor kB Axis: Implications in Inflammatory Disc Disease. Am. J. Pathol. 2014-07-22 [PMID: 25063530] (WB, Rat)
  
  Details:
  Glyceraldehyde-3-phosphate dehydrogenase / GAPDH antibody used for WB in TNF-alpha and IL-1 beta treated Rat's Nucleus pulposus/NP cells (Figure 1E, Figure 6A/E); TNF-alpha treated human NP cells transduced with either Sdc4-shRNA (LV-shSDC4) or Ctr-shRNA (LV-shC) (Figure 2C and E); human NP cells transduced with LV co-expressing YFP and either shp65 or shIkk beta (Figure 7C/E); TNF alpha/beta treated rat NP cells (Figure 8D).
• Palsamy P, Bidasee KR, Shinohara T. Selenite cataracts: Activation of endoplasmic reticulum stress and loss of Nrf2/Keap1-dependent stress protection. Biochim. Biophys. Acta 2014-07-02 [PMID: 24997453] (WB, Human)
  
  Details:
  GAPDH antibody used for WB on lysates of human lens epithelial cells (HLECs) treated or not with 10 uM sodium selenite for various time periods (Fig 6, Fig 7, Fig 8, Fig 11).
• Roovers D Developing a reactivatable model for for HIV-1 Env expression mimicking latent infection Thesis 2022-01-01
• Alidousty C, Rauen T, Hanssen L et al. Calcineurin-mediated YB-1 dephosphorylation regulates CCL5 expression during monocyte differentiation. J. Biol. Chem. 2014-06-19 [PMID: 24947514] (WB, Rat, Mouse, Human)
  
  Details:
  Used in WB of GAPDH to ensure equal protein loading of whole cell lysates or cytoplasmatic extracts from Human primary monocytes, THP-1 cells, HEK293 cells, rat mesangial cells (rMCs) and kidney of C57BL/6 mice.
• Holmes O, Paturi S, Selkoe DJ, Wolfe MS. Pen-2 Is Essential for gamma-Secretase Complex Stability and Trafficking but Partially Dispensable for Endoproteolysis Biochemistry. 2014-06-30 [PMID: 24941111] (WB, Mouse)
  
  Details:
  GAPDH antibody used as loading control in WB on lysates of Pen-2 knockout mouse embryonic fibroblasts/MEFs. Figure 6a - WB on stable cell lines of select Pen-2 mutants - monoclonal cell lines expressing wt, N8A, P27A, N33A, or I53A F-Pen-2; Figure 8 - WB on Pen-2 KO MEFs rescued with mutants of Pen-2 and treated with 8h - 10 uM MG132 ( a peptide aldehyde that effectively block the proteolytic activity of the 26S proteasome complex); Figure 10a - WB on Pen-2 KO MEFs treated with 10 uM MG132 or vehicle.
• McCord MC, Kullmann PH, He K et al. Syntaxin-binding domain of Kv2.1 is essential for the expression of apoptotic K+ currents. J. Physiol. (Lond.). 2014-06-13 [PMID: 24928958] (WB, Chinese Hamster)
  
  Details:
  Figure 3 C - Chinese hamster ovary (CHO) cells expressing syntaxin and Kv2.1 in the absence or presence of peptide C1a441–522
• Chelluboina B, Klopfenstein JD, Pinson DM et al. Stem Cell Treatment After Cerebral Ischemia Regulates the Gene Expression of Apoptotic Molecules Neurochem. Res. 2014-05-31 [PMID: 24879430] (WB, Rat)
  
  Details:
  GAPDH antibody used as a loading control for WB on the brain tissues of male Sprague–Dawley rats - sham controls and untreated or hUCBSCs/human umbilical cord blood-derived mesenchymal stem cells - treated ischemic brains (experimental MCAO/middle cerebral artery occlusion model intravenously injected or not with hUCBSCs). WB image in Fig. 4
• Palsamy P, Bidasee KR, Ayaki M et al. Methylglyoxal induces endoplasmic reticulum stress and DNA demethylation in the Keap1 promoter of human lens epithelial cells and age-related cataracts. Free Radic. Biol. Med. 2014-04-11 [PMID: 24746615] (WB, Human)
• Wang SH, Cheng CY, Chen CJ et al. Changes in protein expression in testes of L2 strain Taiwan country chickens in response to acute heat stress. Theriogenology 2014-03-18 [PMID: 24725420] (WB, Chicken)
• Steppan J, Sikka G, Jandu S et al. Exercise, vascular stiffness, and tissue transglutaminase. J Am Heart Assoc 2014-04-16 [PMID: 24721796] (WB, Rat, Mouse)
• Crapser Jd Investigating microglial regulation of the extracellular matrix in health and neurodegenerative disease Award 2021-01-01 (WB)
• England E M, Shi H et al. Chronic activation of AMP-activated protein kinase increases monocarboxylate transporter 2 and 4 expression in skeletal muscle. J Anim Sci 2017-01-08 [PMID: 28805903] (WB, Porcine)
• Vitte J, Gao F, Coppola G et al. Timing of Smarcb1 and Nf2 inactivation determines schwannoma versus rhabdoid tumor development Nat Commun 2017-08-21 [PMID: 28824165] (WB, Mouse)
• Zak TJ, Koshman YE, Samarel AM, Robia SL. Regulation of Focal Adhesion Kinase through a Direct Interaction with an Endogenous Inhibitor. Biochemistry 2017-08-23 [PMID: 28782937]
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  Details:
  This GAPDH antibody was used as a control in a Western Blot experiment to show an increase in neuronal cyclin E1 expression induced by chemical ischemia.
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  Details:
  Using the PE conjugated version of NBP1-43352, catalog number NBP1-43352PE.
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  Details:
  WB: ATF6 (IMG-273) & GAPDH (IMG-5019A-1). Nuclear fractions of mouse uterus, Fig 6. Note: ATF6 (IMG-273) was seen at ~36 kDa, Fig 6.
• Tian X, Vroom C, Ghofrani H et al. Phosphodiesterase 10A upregulation contributes to pulmonary vascular remodeling PLoS One 2011-04-11 [PMID: 21494592] (WB, Rat)
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  Details:
  GAPDH-Loading Control (IMG-5019A-1). WB & IHC (frozen): P7 wild type and RGE -/- chicken retina, Fig 1B & Fig 1C.
• Swayne LA, Sorbara CD, Bennett SA. Pannexin 2 is expressed by postnatal hippocampal neural progenitors and modulates neuronal commitment. J Biol Chem. 2010-08-06 [PMID: 20529862] (WB)
  
  Details:
  WB: Neural stem and progenitor (NPC) cells isolated from postnatal day 3 C57BL/6 mouse hippocampus, Fig 1A. WB: Neuronal cells isolated from 14 day old C57BL/6 mouse neurospheres, Fig 2C & 3C.
• Hirai A, Ohtsuka N, Ikeda T et al. Role of mouse hepatitis virus (MHV) receptor murine CEACAM1 in the resistance of mice to MHV infection: studies of mice with chimeric mCEACAM1a and mCEACAM1b. J Virol. 2010-07-01 [PMID: 20410265] (WB, Mouse)
  
  Details:
  WB: The liver, spleen, and ileum of MHV-A59 infected mice, Fig 3A.
• Cote MC, Lavoie JR, Houle F et al. Regulation of vascular endothelial growth factor-induced endothelial cell migration by LIM kinase 1-mediated phosphorylation of annexin 1. J Biol Chem. 2010-03-12 [PMID: 20061392] (WB, Human)
• Chui JJ, Li MW, Di Girolamo N et al. Iris pigment epithelial cells express a functional lipopolysaccharide receptor complex. Invest Ophthalmol Vis Sci. 2010-05-01 [PMID: 20019359] (WB, Human)
  
  Details:
  GAPDH (IMG-5019A-1): WB, human ocular pigment epithelial and normal aqueous cells, Fig 3.
• a DW, Steinmetz AG, Kocinsky HS. NHE3 function and phosphorylation are regulated by a calyculin A-sensitive phosphatase. Am J Physiol Renal Physiol;298(3):F745-53. 2010-03-01 [PMID: 20015946] (WB, Primate, Opossum)
• Holden P, Horton WA. Crude subcellular fractionation of cultured mammalian cell lines. BMC Res Notes 2009-12-10 [PMID: 20003239] (WB, Human)
• Zhu ML, Kyprianou N. Role of androgens and the androgen receptor in epithelial-mesenchymal transition and invasion of prostate cancer cells. FASEB J. 2009-11-12 [PMID: 19901020] (WB, Human)
• Costford SR, Bajpeyi S, Pasarica M et al. Skeletal muscle NAMPT is induced by exercise in humans. Am J Physiol Endocrinol Metab 2010-01-01 [PMID: 19887595] (WB, Human)
• Kang YS, Zhao X, Lovaas J et al. Clathrin-independent internalization of normal cellular prion protein in neuroblastoma cells is associated with the Arf6 pathway. J Cell Sci;122(Pt 22):4062-9. 2009-11-15 [PMID: 19843586] (WB, Mouse)
• Zhao J, Zhang Y, Ithychanda SS et al. Migfilin interacts with Src and contributes to cell-matrix adhesion-mediated survival signaling. J Biol Chem;284(49):34308-20. 2009-12-04 [PMID: 19833732] (ICC/IF, Human)
• Kolosionek E, Savai R, Ghofrani HA et al. Expression and activity of phosphodiesterase isoforms during epithelial mesenchymal transition: the role of phosphodiesterase 4. Mol Biol Cell;20(22):4751-65. 2009-11-01 [PMID: 19759179] (WB, Human)
• Fox JT, Shin WK, Caudill MA et al. A UV-responsive internal ribosome entry site enhances serine hydroxymethyltransferase 1 expression for DNA damage repair. J Biol Chem;284(45):31097-108. 2009-11-06 [PMID: 19734144] (WB, Human)
• Fox JT, Stover PJ. Mechanism of the internal ribosome entry site-mediated translation of serine hydroxymethyltransferase 1. J Biol Chem;284(45):31085-96. 2009-11-06 [PMID: 19734143] (WB, Human)
• Jung YW, Kim YT, Kim SW et al. Correlation of human leukocyte antigen-G (HLA-G) expression and disease progression in epithelial ovarian cancer. Reprod Sci;16(11):1103-11. 2009-11-01 [PMID: 19692629] (WB, Human)
• Catalgol B, Ziaja I, Breusing N et al. The Proteasome Is an Integral Part of Solar Ultraviolet A Radiation-induced Gene Expression. J Biol Chem;284(44):30076-30086. 2009-01-01 [PMID: 19690165] (WB, Human)
• Kim JH, Bugaj LJ, Oh YJ et al. Arginase inhibition restores NOS coupling and reverses endothelial dysfunction and vascular stiffness in old rats. J Appl Physiol;107(4):1249-1257. 2009-01-01 [PMID: 19661445] (WB, Rat)
• Matsui Y, Hadaschik BA, Fazli L et al. Intravesical combination treatment with antisense oligonucleotides targeting heat shock protein-27 HTI-286 as a novel strategy for high-grade bladder cancer. Mol Cancer Ther;8(8):2402-2411. 2009-01-01 [PMID: 19625496] (WB, Mouse)
• Guerreiro JR, Lameu C, Oliveira EF et al. Argininosuccinate Synthetase Is a Functional Target for a Snake Venom Anti-hypertensive Peptide: ROLE IN ARGININE NITRIC OXIDE PRODUCTION. J Biol Chem;284(30):20022-20033. 2009-01-01 [PMID: 19491403] (WB, Human)
• Sudarshan S, Sourbier C, Kong H-S et al. Fumarate Hydratase Deficiency in Renal Cancer Induces Glycolytic Addiction Hypoxia-Inducible Transcription Factor 1{alpha} Stabilization by Glucose-Dependent Generation of Reactive Oxygen Species. Mol Cell Biol;29(15):4080-4090. 2009-01-01 [PMID: 19470762] (WB, Human)
• Ding L, Paszkowski-Rogacz M, Nitzsche A et al. A genome-scale RNAi screen for Oct4 modulators defines a role of the Paf1 complex for embryonic stem cell identity. Cell Stem Cell 2009-05-08 [PMID: 19345177] (WB, Mouse)
• Kang Q, Wang T, Zhang H et al. A Golgi-associated protein 4.1B variant is required for assimilation of proteins in the membrane. J Cell Sci. 2009-04-15 [PMID: 19299464] (IP, Human)
  
  Details:
  IP (human bronchial epithelial cells), Fig. 7A.
• Hynynen R, Suchanek M, Spl J et al. OSBP-related protein 2 is a sterol receptor on lipid droplets that regulates the metabolism of neutral lipids. J Lipid Res;50(7):1305-1315. 2009-01-01 [PMID: 19224871] (ICC/IF, Human)
• Zhao J et al. Interleukin-13-induced MUC5AC is regulated by 15-lipoxygenase 1 pathway in human bronchial epithelial cells. Am J Respir Crit Care Med;179(9):782-90. 2009-05-01 [PMID: 19218191] (WB, Human)
• Dong C, Kwas C, Wu L. Transcriptional restriction of human immunodeficiency virus type 1 gene expression in undifferentiated primary monocytes. J Virol. 2009-04-01 [PMID: 19211771] (WB, Human)
  
  Details:
  WB (human monocytes), Figs. 5A,C; 7A.
• Devalaraja-Narashimha K, Padanilam BJ. PARP-1 inhibits glycolysis in ischemic kidneys. J Am Soc Nephrol;20(1):95-103. 2009-01-01 [PMID: 19056868] (WB, Mouse)
• Woodruff TM et al. The complement factor C5a contributes to pathology in a rat model of amyotrophic lateral sclerosis. J Immunol;181(12):8727-34. 2008-12-15 [PMID: 19050293] (WB, Rat)
• MacFarlane AJ et al. Mthfd1 is an essential gene in mice and alters biomarkers of impaired one-carbon metabolism. J Biol Chem;284(3):1533-9. 2009-01-16 [PMID: 19033438] (WB, Mouse)
• Klein M, Schermuly RT, Ellinghaus P et al. Combined tyrosine and serine/threonine kinase inhibition by sorafenib prevents progression of experimental pulmonary hypertension and myocardial remodeling. Circulation;118(20):2081-90. 2008-11-11 [PMID: 18955668] (WB, Rat)
• Ezhilarasan Ravesanker, Jadhav Unmesh, Mohanam Indra et al. The hemopexin domain of MMP-9 inhibits angiogenesis and retards the growth of intracranial glioblastoma xenograft in nude mice. International Journal of Cancer 2009-01-01 [PMID: 18942717] (WB, Human)
• Singh A, Boldin-Adamsky S, Thimmulappa RK et al. RNAi-mediated silencing of nuclear factor erythroid-2-related factor 2 gene expression in non-small cell lung cancer inhibits tumor growth and increases efficacy of chemotherapy. Cancer Res. 2008-10-01 [PMID: 18829555] (WB, Human)
  
  Details:
  WB (human A549 and H460 cells), Fig. 1.
• Schmitt R, Marlier A, Cantley LG. Zag expression during aging suppresses proliferation after kidney injury. J Am Soc Nephrol;19(12):2375-83. 2008-12-01 [PMID: 18815245] (WB, Mouse)
• Monteghirfo S et al. Antileukemia effects of xanthohumol in Bcr/Abl-transformed cells involve nuclear factor-kappaB and p53 modulation. Mol Cancer Ther;7(9):2692-702. 2008-09-01 [PMID: 18790751] (WB, Human)
• Vene R et al. Glycogen synthase kinase 3beta regulates cell death induced by synthetic triterpenoids. Cancer Res;68(17):6987-96. 2008-09-01 [PMID: 18757413] (WB, Human)
• Wee S, Wiederschain D, Maira S-M et al. PTEN-deficient cancers depend on PIK3CB. Proc Natl Acad Sci U S A;105(35):13057-62. 2008-09-02 [PMID: 18755892] (WB, Human)
• Smith HW, Marra P, Marshall CJ. uPAR promotes formation of the p130Cas-Crk complex to activate Rac through DOCK180. J Cell Biol;182(4):777-90. 2008-08-25 [PMID: 18725541] (WB, Human)
• Alkam T, Nitta A, Mizoguchi H et al. The extensive nitration of neurofilament light chain in the hippocampus is associated with the cognitive impairment induced by amyloid beta in mice. J Pharmacol Exp Ther. 2008-10-01 [PMID: 18621989] (WB, Mouse)
  
  Details:
  WB (mouse hippocampal lysates), Fig. 7A.
• Macartney-Coxson DP, Hood KA, Shi HJ et al. Metastatic susceptibility locus, an 8p hot-spot for tumour progression disrupted in colorectal liver metastases: 13 candidate genes examined at the DNA, mRNA and protein level. BMC Cancer. 2008-07-01 [PMID: 18590575] (WB, Human)
  
  Details:
  GAPDH (IMG-5019A). WB: Human colon normal, colon tumour, and liver metastasis, Fig 5.
• Turnquist HR et al. IL-1beta-driven ST2L expression promotes maturation resistance in rapamycin-conditioned dendritic cells. J Immunol;181(1):62-72. 2008-07-01 [PMID: 18566370] (WB, Mouse)
• Chetty C, Lakka SS, Bhoopathi P et al. Tissue inhibitor of metalloproteinase 3 suppresses tumor angiogenesis in matrix metalloproteinase 2-down-regulated lung cancer. Cancer Res;68(12):4736-45. 2008-06-15 [PMID: 18559520] (WB, Human)
• Bass-Zubek AE et al. Plakophilin 2: a critical scaffold for PKC alpha that regulates intercellular junction assembly. J Cell Biol;181(4):605-13. 2008-05-19 [PMID: 18474624] (WB, Human)
• Diaz Encarnacion MM et al. Signaling pathways modulated by fish oil in salt-sensitive hypertension. Am J Physiol Renal Physiol;294(6):F1323-35. 2008-06-01 [PMID: 18385269] (WB, Rat)
• Zhang QS et al. Tempol protects against oxidative damage and delays epithelial tumor onset in Fanconi anemia mice. Cancer Res;68(5):1601-8. 2008-03-01 [PMID: 18316625] (WB, Mouse)
• Chen, K, Y Tu et al. PINCH-1 regulates the ERK-Bim pathway and contributes to apoptosis resistance in cancer cells. J Biol Chem. 283(5):2508-17. 2008-02-01 [PMID: 18063582] (WB, Human)
• Tsai WJ, McCormick KM, Brazeau DA et al. Estrogen effects on skeletal muscle insulin-like growth factor 1 and myostatin in ovariectomized rats. Exp Biol Med (Maywood). 2007-11-01 [PMID: 17959844] (WB, Rat)
  
  Details:
  WB (used as a gel loading control for rat muscle tissue lysates): Figs 6C, 7C, 8C.
• Limes, S W, P J Rozance et al. Increased insulin sensitivity and maintenance of glucose utilization rates in fetal sheep with placental insufficiency and intrauterine growth restriction. Am J Physiol Endocrinol Metab;293(6):E1716-25. 2007-12-01 [PMID: 17895285] (WB, Sheep)
• Banerjee PP, Pandey R, Zheng R et al. Cdc42-interacting protein-4 functionally links actin and microtubule networks at the cytolytic NK cell immunological synapse. J Exp Med. 2007-10-01 [PMID: 17785506] (WB)
  
  Details:
  WB (Fig 8A): Protein standardization for siRNA studies.
• Zhao N. Notch Signaling Guides Vascular Smooth Muscle Cell Function. Thesis. 2014-01-01 (WB, Human, Mouse)
• Cui, W, E Cuartas et al. CD200 and its receptor, CD200R, modulate bone mass via the differentiation of osteoclasts. Proc Natl Acad Sci U S A;104(36):14436-41. 2007-09-04 [PMID: 17726108] (WB, Mouse)
• Woeller CF, Fox JT, Perry C, Stover PJ. A ferritin-responsive internal ribosome entry site regulates folate metabolism. J Biol Chem;282(41):29927-35. 2007-10-12 [PMID: 17702748] (WB, Human)
• Porchia, LM et al. 5-(2-phenanthrenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]-phenyl} acetamide (OSU-03012), a celecoxib derivative, directly targets p21-activated kinase. Mol Pharmacol. 72(5):1124-31. Epub 2007 Aug 2. 2007-11-01 [PMID: 17673571] (WB, Human)
• Zhou M, Xia L, Wang J. Metformin transport by a newly cloned proton-stimulated organic cation transporter (plasma membrane monoamine transporter) expressed in human intestine. Drug Metab Dispos. 2007-10-01 [PMID: 17600084] (WB, Human)
  
  Details:
  Products cited for WB (human small intestine, Fig 6B): 1. GAPDH (IMG-5019A-1, -2) 2. INSTA-BLOT containing human small intenstine tissue lysate (IMB-103, IMB-123).
• Mao W, Fukuoka S, Iwai C et al. Cardiomyocyte apoptosis in autoimmune cardiomyopathy: mediated via endoplasmic reticulum stress and exaggerated by norepinephrine. Am J Physiol Heart Circ Physiol. 2007-09-01 [PMID: 17545481] (WB, Rabbit)
  
  Details:
  Antibodies cited: 1. ATF6 (IMG-273): WB (Fig 7), analysis of ATF6 in nuclear fraction of rabbit left ventricle myocardium tissue lysates, Authors describe cleaved forms of p90ATF6 in the nucleus: p60ATF6 and p36ATF6. 2. GAPDH (IMG-5019A): WB (Fig 4), GAPD
• Blight KJ. Allelic variation in the hepatitis C virus NS4B protein dramatically influences RNA replication. J Virol. 2007-06-01 [PMID: 17360748] (WB, Human)
  
  Details:
  Antibodies cited: 1. GAPDH (IMG-5019A-1, IMG-5019A-2) [WB, Fig7, Fig8 (Huh-7.5 human hepatoma cell line)].
• Redman PT, He K, Hartnett KA et al. Apoptotic surge of potassium currents is mediated by p38 phosphorylation of Kv2.1. Proc Natl Acad Sci USA 2007-02-27 [PMID: 17360683] (WB, Rat)
  
  Details:
  This GAPDH antibody was used as a loading control in a Western Blot experiment to show injury from oxidation increases S800 phosphorylation in cortical neurons.
• Block, K et al. NAD(P)H oxidases regulate HIF-2alpha protein expression. J Biol Chem;282(11):8019-26. 2007-03-16 [PMID: 17200123] (WB, Human)
• Xia L, Engel K, Zhou M et al. Membrane localization and pH-dependent transport of a newly cloned organic cation transporter (PMAT) in kidney cells. Am J Physiol Renal Physiol. 2007-02-01 [PMID: 17018840]
• Wang G, Wu WW, Pisitkun T et al. Automated quantification tool for high-throughput proteomics using stable isotope labeling and LC-MSn. Anal Chem 2006-08-15 [PMID: 16906720]
• Fatma, Sumbul et al. Research Articles: Pluronic L81 enhances triacylglycerol accumulation in the cytosol ingibits chylomicron secretion. 47: 2422-2432, 2006. [PMID: 16894241] (WB, Human)
• Ju, J-S et al. Potentiation of insulin-stimulated glucose transport by the AMP-activated protein kinase. Am J Physiol Cell Physiol;292(1):C564-72. 2007-01-01 [PMID: 16870829] (WB, Mouse)
• Kanadia, R N et al. Reversal of RNA missplicing and myotonia after muscleblind overexpression in a mouse poly(CUG) model for myotonic dystrophy. Proc Natl Acad Sci U S A;103(31):11748-53. 2006-08-01 [PMID: 16864772] (WB, Mouse)
• Iwatani M, Ikegami K, Kremenska Y et al. Dimethyl sulfoxide has an impact on epigenetic profile in mouse embryoid body. Stem Cells. 2006-11-01 [PMID: 16840553]
• Shibata, Yoshimi et al. Heat-killed BCG induces biphasic cyclooxygenase 2+ splenic macrophage formation--role of IL-10 and bone marrow precursors. J Leukoc Biol;80(3):590-8. 2006-09-01 [PMID: 16822852] (WB, Mouse)
• Le Boeuf F, Houle F, Sussman M et al. Phosphorylation of focal adhesion kinase (FAK) on Ser732 is induced by rho-dependent kinase and is essential for proline-rich tyrosine kinase-2-mediated phosphorylation of FAK on Tyr407 in response to vascular endothelial growth factor. Mol Biol Cell;17(8):3508-20. 2006-08-01 [PMID: 16760434] (WB, Human)
• Barry, JS et al. Developmental changes in ovine myocardial glucose transporters and insulin signaling following hyperthermia-induced intrauterine fetal growth restriction. Exp Biol Med (Maywood);231(5):566-75. 2006-05-01 [PMID: 16636305] (WB, Sheep)
• Azfer, A et al. Activation of endoplasmic reticulum stress response during the development of ischemic heart disease. Am J Physiol Heart Circ Physiol;291(3):H1411-20. 2006-09-01 [PMID: 16617122] (WB, Mouse)
• Arthur DB et al. Inhibition of apoptosis by P2Y2 receptor activation: novel pathways for neuronal survival. J Neurosci;26(14):3798-804. 2006-04-05 [PMID: 16597733] (WB, Mouse)
• Pisitkun, T et al. High-throughput identification of IMCD proteins using LC-MS/MS. Physiol Genomics;25(2):263-76. 2006-04-13 [PMID: 16449382] (WB, Rat)
• Jia, Y Takimoto, K Jia Y, Takimoto K. Mitogen-activated protein kinases control cardiac KChIP2 gene expression. Circ Res;98(3):386-93. 2006-02-17 [PMID: 16385079] (WB, Rat)
• Godsel, LM et al. Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. J Cell Biol;171(6):1045-59. 2005-12-19 [PMID: 16365169] (WB, Human)
• Dusek, R L et al. The differentiation-dependent desmosomal cadherin desmoglein 1 is a novel caspase-3 target that regulates apoptosis in keratinocytes. J Biol Chem;281(6):3614-24. 2006-02-10 [PMID: 16286477] (WB, Human)
• Liu X, Kvetnansky R, Serova L et al. Increased susceptibility to transcriptional changes with novel stressor in adrenal medulla of rats exposed to prolonged cold stress. Brain Res Mol Brain Res. 2005-11-18 [PMID: 16169632]
• Pendelton, LC et al. Regulation of endothelial argininosuccinate synthase expression and NO production by an upstream open reading frame. J Biol Chem;280(25):24252-60. 2005-06-24 [PMID: 15851478] (WB, Bovine)
• Sarkar, S et al. Myocardial cell death and regeneration during progression of cardiac hypertrophy to heart failure. J Biol Chem;279(50):52630-42. 2004-12-10 [PMID: 15385543] (WB, Rat, Human)
• Gonzales-Cabrera, P et al. Differential regulation of the cell cycle by alpha1-adrenergic receptor subtypes. Endocrinology;145(11):5157-67. 2004-11-01 [PMID: 15297446] (WB, Human, Rat)
• Moshynska, O et al. Prognostic significance of a short sequence insertion in the MCL-1 promoter in chronic lymphocytic leukemia. J Natl Cancer Inst;96(9):673-82. 2004-05-05 [PMID: 15126604] (WB, Human)
• Akassoglou, K et al. Brain-specific deletion of neuropathy target esterase/swisscheese results in neurodegeneration. Proc Natl Acad Sci U S A;101(14):5075-80. 2004-04-06 [PMID: 15051870] (WB, IF/IHC, ICC/IF, Mouse)
• Ellis RC, Earnhardt JN, Hayes RL et al. Cathepsin B mRNA and protein expression following contusion spinal cord injury in rats. J Neurochem. 2004-02-01 [PMID: 14720218] (WB)
  
  Details:
  WB: GAPDH used as a protein loading control (rat spinal cord tissue), Fig. 3a,b,c.
• Fortun J, Dunn WA Jr, Joy S et al. Emerging role for autophagy in the removal of aggresomes in Schwann cells. J Neurosci. 2003-11-19 [PMID: 14627652] (WB, Mouse)
  
  Details:
  WB: GAPDH used as a protein loading control (Mouse Schwann cell lysates), Fig. 3F.
• Kapadia SB, Brideau-Andersen A, Chisari FV et al. Interference of hepatitis C virus RNA replication by short interfering RNAs. Proc Natl Acad Sci U S A. 2003-02-01 [PMID: 12566571] (WB, ICC/IF, Human)

Reviews for GAPDH Antibody (NB300-221) (19)

Average Rating: 4.4

(Based on 19 reviews)

We have 19 reviews tested in 6 species: Human, Mouse, Rat, Human and Mouse, Other, cat.

reviewed by: Verified Customer WB Human 06/14/2021

Summary

• Application: Western Blot
• Sample Tested: eye tissue
• Species: Human
• Lot: 082219

Comments

• Comments: Use at 1:500

reviewed by: Verified Customer WB Human 01/31/2019

Summary

• Application: Western Blot
• Sample Tested: Human nucleus pulposus cell culture
• Species: Human
• Lot: 111713

reviewed by: Wan-Ching Cheng WB cat 09/13/2018

Summary

• Application: Western Blot
• Sample Tested: Heart tissue
• Species: cat
• Lot: 083017

reviewed by: Verified Customer WB Human and Mouse 06/29/2017

Summary

• Application: Western Blot
• Sample Tested: 293FT cell lysate,nih3t3 cell lysate
• Species: Human and Mouse
• Lot: 120116

reviewed by: James Xiao WB Mouse 05/22/2017

Summary

• Application: Western Blot
• Sample Tested: Mouse lung endothelial cells
• Species: Mouse
• Lot: 012517

Comments

• Comments: Cell lysates of treated mouse lung endothelial cells were analyzed by western blotting.

reviewed by: Verified Customer WB Human 01/14/2015

Summary

• Application: Western Blot
• Sample Tested: Human nucleus pulposus cell culture
• Species: Human
• Lot: 111711

reviewed by: Verified Customer WB Other 01/09/2015

Summary

• Application: Western Blot
• Sample Tested: Chicken Pectoralis Major
• Species: Other
• Lot: 111711

reviewed by: Eric England WB Other 10/08/2014

Summary

• Application: Western Blot
• Sample Tested: Whole Skeletal Muscle Lysate
• Species: Other
• Lot: 111711

reviewed by: Liyong Zhang WB Mouse 07/01/2014

Summary

• Application: Western Blot
• Species: Mouse

reviewed by: Verified Customer WB Other 12/16/2013

Summary

• Application: Western Blot
• Sample Tested: Zebrafish embryo lysates
• Species: Other

reviewed by: Verified Customer WB Human 12/23/2011

Summary

• Application: Western Blot
• Sample Tested: Human
• Species: Human

reviewed by: Verified Customer WB Human 12/21/2011

Summary

• Application: Western Blot
• Sample Tested: Human Hepatoblastoma
• Species: Human

reviewed by: Verified Customer WB Rat 12/06/2011

Summary

• Application: Western Blot
• Sample Tested: Rat
• Species: Rat

reviewed by: Verified Customer WB Human 12/05/2011

Summary

• Application: Western Blot
• Sample Tested: Human
• Species: Human

reviewed by: Verified Customer WB Rat 11/23/2011

Summary

• Application: Western Blot
• Sample Tested: Rat brain
• Species: Rat

reviewed by: Verified Customer WB Rat 11/21/2011

Summary

• Application: Western Blot
• Sample Tested: Rat
• Species: Rat

reviewed by: Verified Customer WB Human 09/30/2011

Summary

• Application: Western Blot
• Sample Tested: Human
• Species: Human

reviewed by: Verified Customer WB Other 03/25/2010

Summary

• Application: Western Blot
• Sample Tested: CHO cells, Sample Amount: 20ug
• Species: Other
• Lot: 113003

reviewed by: Verified Customer WB Human 06/04/2009

Summary

• Application: Western Blot
• Sample Tested: EAhy926 cell lysate, Sample Amount: 15ug
• Species: Human
• Lot: P1

FAQs for GAPDH Antibody (NB300-221). (Showing 1 - 7 of 7 FAQs).

1. We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?
   • GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.
2. I want to know why GAPDH is used as a loading control antibody in WB techniques.
   • Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.
3. I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?
   • This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.
4. I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?
   • Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.
5. What secondary antibody should I use for visualization of the GAPDH antibody?
   • This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.
6. Do you offer a smaller size for the GAPDH antibodies?
   • We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.
7. I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.
   • For homogenized tissue, beta-actin and GAPDH are both fine.

Bioinformatics

• Gene Symbol: GAPDH
• Entrez:
  • 2597(Human)
  • 14433(Mouse)
  • 24383(Rat)
• Uniprot:
  • P00355(Porcine)