HeLa Whole Cell Lysate

Images

 
Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of HeLa whole cell lysate (NB800-PC1) using p14ARF antibody (NB200-111) with ECL detection method.
Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - Ponceau S staining of HeLa whole cell lysate using NB800-PC1.
Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - Analysis of STIM-1 antibody (NB110-60547) using HeLa whole cell lysate [NB800-PC1].
Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of 30ug of HeLa whole cell lysate (NB800-PC1) using rabbit polyclonal Survivin antibody (NB 500-201) at 1ug/ml concentration. Detection was performed using ...read more
Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of HeLa whole cell lysate (NB800-PC1) using rabbit polyclonal NBS1 antibody (NB100-143) with detection employing ECL method.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
2.5 mg/ml

Order Details

Applications/Dilutions

Application Notes
This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Standard RIPA buffer including protease inhibitors and 0.1% SDS diluted in 1 X lithium dodecyl sulfate (LDS) sample buffer with bromophenol blue and 40-70% glycerol, pH 8.4. Denaturing agents have not been added.
Preservative
No Preservative
Concentration
2.5 mg/ml

Lysate Details for HeLa

Type
Cell
Tissue
Reproductive
Subcellular Fraction
Whole

Background

HeLa whole cell lysate was lysed in modified RIPA buffer (150 mM sodium chloride, 25 mM Tris-HCl, pH 7.6, 1% Triton X-100, 1% sodium deoxycholic acid, 1% sodium dodecyl sulfate, 1 mM phenyl methyl sulfonyl fluoride, 1 ug/ml of aprotinin, 1 ug/ml of leupeptin, 1ug/ml of pepstatin). Protein concentration was determined with Bio-Rad protein assay.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

WB Video Protocol

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HeLa NB800-PC1

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