14-3-3 zeta Antibody (818515) [Unconjugated]

Images

 
Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line,SK‑Mel‑28 human malignant melanoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line. PVDF membrane was ...read more
Simple Western lane view shows lysates of SH‑SY5Y human neuroblastoma cell line, loaded at 0.5 mg/mL. A specific band was detected for 14‑3‑3 zeta at approximately 34 kDa (as indicated) using 2 µg/mL of Mouse ...read more
14‑3‑3 zeta was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human 14‑3‑3 zeta Monoclonal Antibody (Catalog # MAB2669) at 15 µg/mL overnight at 4 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clone
818515
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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14-3-3 zeta Antibody (818515) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human 14-3-3 zeta
Asp2-Asn245
Accession # P63104
Specificity
Detects human 14-3-3 zeta in direct ELISAs and Western blots. Detects Mouse and Rat 14-3-3 zeta in Western Blots. In direct ELISAs, no
cross-reactivity with recombinant human 14-3-3 beta, theta, eta, gamma, sigma, or epsilon is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
YWHAZ
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
  • Simple Western 2 ug/mL
  • Immunohistochemistry 8-25 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 1 Review rated 5
using
MAB2669 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 14-3-3 zeta Antibody (818515) [Unconjugated]

  • 1433 zeta
  • 14-3-3 zeta
  • KCIP-1
  • KCIP-1MGC126532,14-3-3-zeta
  • MGC111427
  • MGC138156
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, deltapolypeptide
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zetapolypeptide
  • YWHAZ
  • zeta polypeptide

Background

14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in high abundance in all eukaryotic cells. 14-3-3 proteins were the first polypeptides shown to have pSer/Thr binding properties, generally recognizing the consensus sequences RSXpSXP and RXY/FXpSXP (where X is any amino acid). 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. For example, the binding of 14-3-3 to phosphorylated BAD blocks its proapoptotic association with Bcl-XL. There are at least seven distinct 14-3-3 genes in vertebrates, alpha/beta, epsilon, eta, gamma, theta, sigma and zeta ( alpha / beta, epsilon, eta, gamma, tau / theta, sigma, and zeta /δ). 14-3-3 zeta, also known as Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, zeta isoform (gene name YWHAZ) is a 245 amino acid, 27 kDa protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for 14-3-3 zeta Antibody (MAB2669) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB2669:
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Images Ratings Applications Species Date Details
Western Blot 14-3-3 zeta MAB2669
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reviewed by:
Michal Dibus
WB Human 08/02/2017
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Summary

ApplicationWestern Blot
Sample TestedHuman recombinant protein from E. Coli
SpeciesHuman
LotCHGN0115081

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Recent Reviews

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Michal Dibus
08/02/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol YWHAZ
Uniprot
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