Ubiquitin Antibody (Ubi-1)


Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - Western blot of Ubiquitin expression on (Lane 1) poly-ubiquitin (lys63 linked), (Lane 2) pure ubiquitin and crude homogenates of adult rat, (Lane 3) cortex, (Lane ...read more
Immunohistochemistry-Paraffin: Ubiquitin Antibody (Ubi-1) [NB300-130] - IHC-P staining of cerebral cortex tissue from an Alzheimer patient brain using Ubiquitin antibody (clone Ubi-1) at 1:1000 dilution. Neurofibrillary ...read more
Immunohistochemistry-Paraffin: Ubiquitin Antibody (Ubi-1) [NB300-130] - Staining of Ubiquitin in hippocampal tissue from an Alzheimer patient.
Immunohistochemistry-Paraffin: Ubiquitin Antibody (Ubi-1) [NB300-130] - IHC staining of Ubiquitin in human rectal cancer using DAB with hematoxylin counterstain.

Product Details

Reactivity Hu, Mu, Rt, Bv, Ce, Ch, Dr, PlSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

Ubiquitin Antibody (Ubi-1) Summary

Purified Ubiquitin conjugated to KLH with glutaraldehyde.
Recognizes polyubiquitin chains much more strongly than monoubiquitinated molecules or free ubiquitin. Specifically recognizes ubiquitinated cytoplasmic inclusion bodies.
IgG1 Kappa
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
10mM Sodium Azide


  • Western Blot 1:1000-1:5000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:500-1:1000
  • Immunohistochemistry-Frozen 1:500-1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
Application Notes
This Ubiquitin Antibody (Ubi-1) is useful for Western blot, ELISA, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on both paraffin-embedded and frozen sections. In Western blot a band can be seen at 8.5 kDa. This antibody is excellent for the detection of ubiquitinated inclusions seen in human neurodegenerative diseases such as the neurofibrillary tangles of Alzheimer's disease. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
8.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
NB300-130 in the following applications:

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NB300-130 in the following applications:

Reactivity Notes

Human, bovine, mouse, rat, chicken, Drosophila, C. elegans, and Arabidopsis thaliana (plant).

Alternate Names for Ubiquitin Antibody (Ubi-1)

  • RPS27A
  • UBA52
  • UBB ubiquitin B
  • UBB
  • UBC


Ubiquitin is a highly conserved protein of about 8.5 kDa molecular weight, which has a normal role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C-terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, GP, Ha, Le, Ma, Pm, Rb, Sh, Sq, Ze, Dr(-)
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
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Species: Hu
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Species: Hu
Applications: WB, IP
Species: Hu, Mu, Po
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Species: Hu, Mu
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Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, GP, Ha, Le, Ma, Pm, Rb, Sh, Sq, Ze, Dr(-)
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Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Pm
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Ubiquitin Antibody (NB300-130)(12)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Drosophila, Plant.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-Fr, WB.

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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB300-130 Applications Species
Pruneda JN, Durkin CH, Geurink PP et al. The Molecular Basis for Ubiquitin and Ubiquitin-like Specificities in Bacterial Effector Proteases Mol. Cell 2016 Jul 21 [PMID:27425412] (WB) WB
Schwab AJ, Ebert AD. Neurite Aggregation and Calcium Dysfunction in iPSC-Derived Sensory Neurons with Parkinson's Disease-Related LRRK2 G2019S Mutation. Stem Cell Reports. 2015 Dec 08 [PMID:26651604] (ICC/IF, Human) ICC/IF Human
Begum G, Yan HQ, Li L et al. Docosahexaenoic Acid Reduces ER Stress and Abnormal Protein Accumulation and Improves Neuronal Function Following Traumatic Brain Injury. J. Neurosci. 3/5/2014 [PMID:24599472] (IHC-Fr, Rat) IHC-Fr Rat
Ejlerskov P, Rasmussen I, Nielsen TT et al. Tubulin Polymerization Promoting Protein (TPPP/p25alpha) promotes unconventional secretion of alpha-synuclein through exophagy by impairing autophagosome-lysosome fusion. J Biol Chem 2013 Apr 29 [PMID:23629650] (ICC/IF, Rat) ICC/IF Rat
Maroui MA, Kheddache-Atmane S, El Asmi F et al. Requirement of PML SUMO Interacting Motif for RNF4- or Arsenic Trioxide-Induced Degradation of Nuclear PML Isoforms PLoS One 2012 [PMID:23028697] (WB, Human) WB Human
Rosenfeldt MT, Nixon C, Liu E et al. Analysis of macroautophagy by immunohistochemistry Autophagy 2012 Jun 1 [PMID:22562096] (IHC, Mouse) IHC Mouse
Stuttmann J, Lechner E, Guerois R et al. COP9 Signalosome- 26S Proteasome-dependent Regulation of SCFTIR1 Accumulation in Arabidopsis. J Biol Chem;284(12):7920-7930. 2009 [PMID:19147500] (WB, Plant) WB Plant
Dayal S, Sparks A, Jacob J et al. Suppression of the Deubiquitinating Enzyme USP5 Causes the Accumulation of Unanchored Polyubiquitin the Activation of p53. J Biol Chem;284(8):5030-41. 2009 Feb 20. [PMID:19098288] (WB, Human) WB Human
Watson MR, Lagow RD, Xu K et al. A Drosophila Model for Amyotrophic Lateral Sclerosis Reveals Motor Neuron Damage by Human SOD1. J Biol Chem;283(36):24972-24981. 2008 [PMID:18596033] (IHC, Drosophila) IHC Drosophila
Chang L, Kamata H, Solinas G et al. The E3 ubiquitin ligase itch couples JNK activation to TNFalpha-induced cell death by inducing c-FLIP(L) turnover. Cell. 2006 Feb 10 [PMID:16469705] (WB) WB
Show All 12 Publications.

Reviews for Ubiquitin Antibody (NB300-130) (1) 31

Average Rating: 3
(Based on 1 reviews)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IF.

Reviews using NB300-130:
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Images Ratings Applications Species Date Details
reviewed by:
IF Mouse 05/27/2009


Sample Testedprimary mouse neuronal culture


Blocking DetailsBlocking Buffer: Fetal Bovie Serun, Blocking Time: 30 minutes, Blocking Temp: 22 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Incubation Dilution Buffer: FBS, Primary Ab Dilution Ratio: 1/1000, Incubation Time: 24hrs, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: Alexa 488

Product General Protocols

View specific protocols for Ubiquitin Antibody (NB300-130): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ubiquitin Antibody (NB300-130). (Showing 1 - 1 of 1 FAQs).

  1. Regarding the WB image on datasheet of NCX1 antibody (6H2) (NB300-127), can you confirm if lane 2 is a rabbit heart sample? In addition, can you provide the dilution fold and loading mount used to obtain this image? Lastly, would you happen to know if t
    • A protein stabilizer has not been added to the antibody NB300-130. However, note that this antibody is supplied as ascites fluid, not as a purified antibody. If you are not familiar with this format, the following descriptions will be useful: Ascites fluid is a complex solution containing multiple proteins and background antibodies as well as the antibody of interest. Ascites contains the antibody produced by the hybridoma cells as well as many other proteins, including background antibodies produced by the mice and albumin. Antibody concentrations in ascites are typically high, but an accurate concentration of the target antibody cannot be readily determined. Therefore, unfortunately you cannot determine the concentration of the ubiquitin antibody within NB300-130 as sold. This is why this product's pack sizes are volumes rather than masses, and why we state on our datasheet: 'This product is unpurified. The exact concentration of antibody is not quantifiable.' All I can suggest is that you carry out antigen affinity chromatography of the antibody, and then determine its concentration.

Secondary Antibodies


Isotype Controls

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Blogs on Ubiquitin.

PINK1: All work and no fun
The protein PINK1 is a mitochondrial-located serine/threonine kinase (PTK) that maintains organelle function and integrity. It not only protects organelles from cellular stress, but it also uses the selective auto-phagocytosis process for cleaning and...  Read full blog post.

Ubiquitin-Mediated Degradation of Cellular Proteins: The Kiss of Death
Ubiquitin is an abundant and essential cellular 9-kd protein that is conserved across evolution from yeast to humans. Ubiquitin is used by cells as a covalent modifier of other proteins both to activate their function and to target them for degradatio...  Read full blog post.

Using Ubiquitin Antibodies in Various Disease Research
Ubiquitin is a small, highly conserved protein which plays an important role in protein breakdown, covalently bonding to proteins to mark them for proteolytic degradation in a process called ubiquitination. Ubiquitin also binds to inclusion bodies (ac...  Read full blog post.

The Heat is On: Heat Shock Proteins and the Link to Cancer
Novus Biologicals offers an extensive antibody catalog targeting heat shock proteins (HSPs). A large protein group covering a number of families, the HSPs are functionally related by their dramatic upregulation in response to stress. Stress triggers m...  Read full blog post.

The Latest Research on IBR-type E3 Ubiquitin Ligases
E3 ubiquitin ligases are standards in most antibody catalogs. These proteins are essential to the process of ubiquitination, which is expressed in protein pathways throughout the body and is often linked to disease states. It is widely used as a bioma...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol RPS27A

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