This Beta Actin antibody is useful for Western Blotting, Immunoprecipitation, and Immunohistochemistry -paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Immunocytochemistry testing in HeLa and NIH3T3 cells did not produce any positive staining. Immunocytochemistry/Immunofluorescence, Blocking/Neutralizing and Enzyme Immunoassay were reported in scientific literature.
Human, Rat, Monkey, Mouse and Avian. Rabbit reactivity reported in the scientific literature (PMID: 23813946). Fish reactivity reported in scientific literature (PMID: 25842206). Expected to cross-react with a wide range of species due to sequence homology.
Packaging, Storage & Formulations
Store at 4C in the dark.
Immunogen affinity purified
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for beta-Actin Antibody [HRP]
actin, cytoplasmic 1
beta cytoskeletal actin
PS1TP5-binding protein 1
Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for beta-Actin Antibody (NB600-503H). (Showing 1 - 6 of 6 FAQs).
Why is beta actin used as a loading control in blotting ?
Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
Are the beta-Actin antibodies validated in Simple Western?
Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
Do Beta-Actin antibodies come in lyophilized format?
ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
What the theoretical molecular weight for Beta-Actin antibodies?
The TMW of beta Actin antibodies is approximately 40 - 42 kDa.
Has Beta Actin Antibody [HRP] (NB600-503H) been tested on brain tissue?
No, we have not tested this antibody specifically in brain. However, beta-actin is expressed in all tissues, so you should not worry about its workability in brain. We do have 48 references for this product, and you may go through these publications to see how other customers have used this product.
What is the immunogen sequence of this Beta-Actin antibody?
A sequence within the N-terminal region of Human beta Actin. The exact sequence is proprietary.
Beta-Actin's Role in Neuronal Plasticity Beta-Actin is a highly conserved protein involved in cell growth, cytoskeletal and extracellular support structures and cell migration. Because beta-Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading contro... Read full blog post.
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