PGK1 Antibody

Images

 
Western Blot: PGK1 Antibody [NBP2-19784] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody diluted by 1:1000.
Immunocytochemistry/ Immunofluorescence: PGK1 Antibody [NBP2-19784] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PGK1 protein stained by PGK1 antibody diluted at 1:20. Red: alpha Tubulin, a ...read more
Immunohistochemistry-Paraffin: PGK1 Antibody [NBP2-19784] - Analysis of paraffin-embedded hepatoma, using antibody at 1:500 dilution.
Western Blot: PGK1 Antibody [NBP2-19784] - PGK1 antibody detects PGK1 protein by Western blot analysis. 50 ug Rat liver lysate/extract, 10% SDS-PAGE, PGK1 antibody dilution: 1:1000.
Biological Strategies: Western Blot: PGK1 Antibody [NBP2-19784] - PGK1 antibody detects PGK1 protein in 1% O2-treated samples by Western blot analysis. Upper panel: PGK1 antibody. Lower panel: beta Actin ...read more
Western Blot: PGK1 Antibody [NBP2-19784] - 50 ug mouse brain whole cell lysate, 10% SDS PAGE, antibody at 1:1000.
Immunocytochemistry/ Immunofluorescence: PGK1 Antibody [NBP2-19784] - Analysis of methanol-fixed HeLa, using antibody at 1:50 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ye, Po, Bv, Pm, RM, ShSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

     

Order Details

PGK1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Porcine (96%), Bovine (96%), Rhesus Macaque (100%), Primate (100%), Sheep (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PGK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:20-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-19784 in the following applications:

Publications
Read Publication using NBP2-19784.

Reactivity Notes

Expected cross reactivity based on sequence homology: Chimpanzee (100%), Zebrafish (87%), Xenopus laevis (84%), Chicken (88%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for PGK1 Antibody

  • Cell migration-inducing gene 10 protein
  • EC 2.7.2.3
  • MGC142128
  • MGC8947
  • MIG10
  • PGK1
  • PGKA
  • PGKAMGC117307
  • phosphoglycerate kinase 1
  • Primer recognition protein 2
  • PRP 2
  • PRP2

Background

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PGK1 Antibody (NBP2-19784)(1)

We have publications tested in 1 application: WB.


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Review for PGK1 Antibody (NBP2-19784) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-19784:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot PGK1 NBP2-19784
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reviewed by:
Min-Joon Han
WB Human 08/16/2018
View

Summary

ApplicationWestern Blot
Sample TestedHuman fibroblast,human iPSC
SpeciesHuman
Lot41878

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PGK1 Antibody (NBP2-19784) (0)

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Recent Reviews

5
5
1
4
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2
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1
0

Min-Joon Han
08/16/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol PGK1
Uniprot