PGK1 Antibody

Western Blot: PGK1 Antibody [NBP2-19784] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: PGK1 Antibody [NBP2-19784] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PGK1 protein stained by PGK1 antibody diluted at 1:20. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000.Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: PGK1 Antibody [NBP2-19784] - Analysis of paraffin-embedded hepatoma, using antibody at 1:500 dilution.

Western Blot: PGK1 Antibody [NBP2-19784] - PGK1 antibody detects PGK1 protein by Western blot analysis. 50 ug Rat liver lysate/extract, 10% SDS-PAGE, PGK1 antibody dilution: 1:1000.

Western Blot: PGK1 Antibody [NBP2-19784] - PGK1 antibody detects PGK1 protein in 1% O2-treated samples by Western blot analysis. Upper panel: PGK1 antibody. Lower panel: beta Actin antibody. A: 30 ug HeLa whole cell lysate/extract (untreated). B: 30 ug HeLa whole cell lysate/extract (1% O2 treatment for 24 hours).

Western Blot: PGK1 Antibody [NBP2-19784] - 50 ug mouse brain whole cell lysate, 10% SDS PAGE, antibody at 1:1000.

Western Blot: PGK1 Antibody [NBP2-19784] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody diluted by 1:1000.

Immunocytochemistry/Immunofluorescence: PGK1 Antibody [NBP2-19784] - Analysis of methanol-fixed HeLa, using antibody at 1:50 dilution.

• Reactivity: Hu, Mu, Rt, Ye, Po, Bv, Pm, RM, Sh
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, PLA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PGK1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Porcine (96%), Chimpanzee (100%), Bovine (96%), Rhesus Macaque (100%), Sheep (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PGK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:20-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation
  • Proximity Ligation Assay
  • Western Blot 1:500-1:3000
• Application Notes: PLA-Assay dependent
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (87%), Xenopus laevis (84%), Chicken (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for PGK1 Antibody

• Cell migration-inducing gene 10 protein
• EC 2.7.2.3
• MGC142128
• MGC8947
• MIG10
• PGK1
• PGKA
• PGKAMGC117307
• phosphoglycerate kinase 1
• Primer recognition protein 2
• PRP 2
• PRP2

Background

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PGK1 Antibody (NBP2-19784)(1)

Publication using NBP2-19784

• Khurana N, Laskar S, Bhattacharyya MK, Bhattacharyya S. Hsp90 induces increased genomic instability towards DNA damaging agents by tuning down RAD53 transcription. Mol. Biol. Cell 2016-06-15 [PMID: 27307581] (WB)

Review for PGK1 Antibody (NBP2-19784) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 08/16/2018

Summary

• Application: Western Blot
• Sample Tested: Human fibroblast,human iPSC
• Species: Human
• Lot: 41878

Bioinformatics

• Gene Symbol: PGK1
• Uniprot:
  • P00558()