Western Blot: PGK1 Antibody [NBP2-19784] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody diluted at 1:3000. The ...read more
Immunocytochemistry/ Immunofluorescence: PGK1 Antibody [NBP2-19784] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PGK1 protein stained by PGK1 antibody diluted at 1:20. Red: alpha Tubulin, a ...read more
Immunohistochemistry-Paraffin: PGK1 Antibody [NBP2-19784] - Analysis of paraffin-embedded hepatoma, using antibody at 1:500 dilution.
Western Blot: PGK1 Antibody [NBP2-19784] - PGK1 antibody detects PGK1 protein by Western blot analysis. 50 ug Rat liver lysate/extract, 10% SDS-PAGE, PGK1 antibody dilution: 1:1000.
Biological Strategies: Western Blot: PGK1 Antibody [NBP2-19784] - PGK1 antibody detects PGK1 protein in 1% O2-treated samples by Western blot analysis. Upper panel: PGK1 antibody. Lower panel: beta Actin ...read more
Western Blot: PGK1 Antibody [NBP2-19784] - 50 ug mouse brain whole cell lysate, 10% SDS PAGE, antibody at 1:1000.
Western Blot: PGK1 Antibody [NBP2-19784] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody diluted by 1:1000.
Immunocytochemistry/ Immunofluorescence: PGK1 Antibody [NBP2-19784] - Analysis of methanol-fixed HeLa, using antibody at 1:50 dilution.
Hu, Mu, Rt, Ye, Po, Bv, Pm, RM, ShSpecies Glossary
45 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NBP2-19784 in the following applications:
Expected cross reactivity based on sequence homology: Chimpanzee (100%), Zebrafish (87%), Xenopus laevis (84%), Chicken (88%).
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS (pH 7), 20% Glycerol
0.025% Proclin 300
Immunogen affinity purified
Alternate Names for PGK1 Antibody
Cell migration-inducing gene 10 protein
phosphoglycerate kinase 1
Primer recognition protein 2
The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.